Lag0014997 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0014997
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cysteine protease XCP1-like
Location	chr12: 6607352 .. 6607811 (+)
RNA-Seq Expression	Lag0014997
Synteny	Lag0014997

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGATTTACCCATTTGATCCATCTGAGCCCGCTGAAGTTGAAGAGGTATGTATGTTTCTTTCGCAGCTCTGTCTGTCTGTAACAAATAGTTTTCCAATCTGATCGAAGATAATATAATTGTTGAATCGTATATAAGATTAACTTGAATTAAGAAGACCCATGAGAAATTTCGGTGGAAAAATTGGTGTTCTGTATCTTGTAGTTGGCTAAAGCAGAGTCTTCGGAGCACTGGGAGGTGTTCAAGTCGTGGATGTCGAAGCACAAAAAGAGTTACAAGGGTGGGAAAGAGATGTTGTATAGGTTTGAGATATTCACTGATACTTTGAAGTACATTGAGGAGAAGAACAAGGAGCTAAAAAGCTATCAACTGGGGTTGAATCAATTCGCAGACTTGAAATTTGATGAGCTTCCTACTGGATTCAAGAGTGGGGGACACAGGATGAGTGGGACACCCTAG

mRNA sequence

ATGCAGATTTACCCATTTGATCCATCTGAGCCCGCTGAAGTTGAAGAGTTGGCTAAAGCAGAGTCTTCGGAGCACTGGGAGGTGTTCAAGTCGTGGATGTCGAAGCACAAAAAGAGTTACAAGGGTGGGAAAGAGATGTTGTATAGGTTTGAGATATTCACTGATACTTTGAAGTACATTGAGGAGAAGAACAAGGAGCTAAAAAGCTATCAACTGGGGTTGAATCAATTCGCAGACTTGAAATTTGATGAGCTTCCTACTGGATTCAAGAGTGGGGGACACAGGATGAGTGGGACACCCTAG

Coding sequence (CDS)

ATGCAGATTTACCCATTTGATCCATCTGAGCCCGCTGAAGTTGAAGAGTTGGCTAAAGCAGAGTCTTCGGAGCACTGGGAGGTGTTCAAGTCGTGGATGTCGAAGCACAAAAAGAGTTACAAGGGTGGGAAAGAGATGTTGTATAGGTTTGAGATATTCACTGATACTTTGAAGTACATTGAGGAGAAGAACAAGGAGCTAAAAAGCTATCAACTGGGGTTGAATCAATTCGCAGACTTGAAATTTGATGAGCTTCCTACTGGATTCAAGAGTGGGGGACACAGGATGAGTGGGACACCCTAG

Protein sequence

MQIYPFDPSEPAEVEELAKAESSEHWEVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDELPTGFKSGGHRMSGTP

Homology

[Close]

BLAST of Lag0014997 vs. NCBI nr
Match: XP_018829301.2 (cysteine protease XCP1-like [Juglans regia])

HSP 1 Score: 81.6 bits (200), Expect = 4.1e-12
Identity = 41/76 (53.95%), Postives = 54/76 (71.05%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDELP 86
           E+F+SWMSKH K YK  +E L RFE+F D LK+I+++NKE+ SY LGLN+FADL  +E  
Sbjct: 81  ELFESWMSKHGKIYKSMEEKLQRFEVFKDNLKHIDQRNKEITSYWLGLNEFADLSHEEFK 140

Query: 87  T---GFKSGGHRMSGT 100
           T   GFK+   R  G+
Sbjct: 141 TQYLGFKTEFPRSRGS 156

[Close]

BLAST of Lag0014997 vs. NCBI nr
Match: XP_038887495.1 (pro-cathepsin H-like [Benincasa hispida])

HSP 1 Score: 81.6 bits (200), Expect = 4.1e-12
Identity = 41/78 (52.56%), Postives = 48/78 (61.54%), Query Frame = 0


Query: 14  VEELAKAESSEHWEVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLG 73
           VE +  A  SE WE FKSWMS H K Y   +EMLYRF +F  TLK IE+ NK       G
Sbjct: 47  VESIYDAVLSEDWESFKSWMSMHNKKYGSEEEMLYRFGLFKKTLKRIEKLNKNYTGCTFG 106

Query: 74  LNQFADLKFDELPTGFKS 92
            N F+DL FDE+P G+ S
Sbjct: 107 FNHFSDLTFDEVPKGYTS 124

[Close]

BLAST of Lag0014997 vs. NCBI nr
Match: KAF5449130.1 (hypothetical protein F2P56_029607 [Juglans regia])

HSP 1 Score: 81.6 bits (200), Expect = 4.1e-12
Identity = 41/76 (53.95%), Postives = 54/76 (71.05%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDELP 86
           E+F+SWMSKH K YK  +E L RFE+F D LK+I+++NKE+ SY LGLN+FADL  +E  
Sbjct: 46  ELFESWMSKHGKIYKSMEEKLQRFEVFKDNLKHIDQRNKEITSYWLGLNEFADLSHEEFK 105

Query: 87  T---GFKSGGHRMSGT 100
           T   GFK+   R  G+
Sbjct: 106 TQYLGFKTEFPRSRGS 121

[Close]

BLAST of Lag0014997 vs. NCBI nr
Match: XP_041012569.1 (cysteine protease XCP1-like [Juglans microcarpa x Juglans regia])

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-11
Identity = 36/63 (57.14%), Postives = 48/63 (76.19%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDELP 86
           E+F+SWMSKH K YK  +E L RFE+F D LK+I+++NKE+ SY LGLN+FADL  +E  
Sbjct: 81  ELFESWMSKHGKIYKSMEEKLQRFEVFKDNLKHIDQRNKEITSYWLGLNEFADLSHEEFK 140

Query: 87  TGF 90
           T +
Sbjct: 141 TQY 143

[Close]

BLAST of Lag0014997 vs. NCBI nr
Match: KAG7978501.1 (hypothetical protein I3843_05G085500 [Carya illinoinensis])

HSP 1 Score: 79.3 bits (194), Expect = 2.0e-11
Identity = 36/63 (57.14%), Postives = 48/63 (76.19%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDELP 86
           E+F+SWMSKH K YK  +E L RFE+F D LK+I+++NKE+ SY LGLN+FADL  +E  
Sbjct: 46  ELFESWMSKHGKIYKSMEEKLQRFEVFKDNLKHIDQRNKEITSYWLGLNEFADLSHEEFK 105

Query: 87  TGF 90
           T +
Sbjct: 106 TQY 108

[Close]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot
Match: P05994 (Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-13
Identity = 35/58 (60.34%), Postives = 43/58 (74.14%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDE 85
           ++F SWM KH K+YK   E LYRFEIF D LKYI+E+NK +  Y LGLN+F+DL  DE
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGYWLGLNEFSDLSNDE 103

[Close]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot
Match: P00784 (Papain OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.9e-12
Identity = 35/58 (60.34%), Postives = 42/58 (72.41%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDE 85
           ++F+SWM KH K YK   E +YRFEIF D LKYI+E NK+  SY LGLN FAD+  DE
Sbjct: 46  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKKNNSYWLGLNVFADMSNDE 103

[Close]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot
Match: O65493 (Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.6e-12
Identity = 30/58 (51.72%), Postives = 46/58 (79.31%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDE 85
           E+F+SWMS+H K+YK  +E ++RFE+F + L +I+++N E+ SY LGLN+FADL  +E
Sbjct: 49  ELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEE 106

[Close]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot
Match: P10056 (Caricain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 9.5e-12
Identity = 34/58 (58.62%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDE 85
           ++F SWM  H K Y+   E LYRFEIF D L YI+E NK+  SY LGLN+FADL  DE
Sbjct: 46  QLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKKNNSYWLGLNEFADLSNDE 103

[Close]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot
Match: P14080 (Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 69.3 bits (168), Expect = 2.8e-11
Identity = 34/58 (58.62%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 27  EVFKSWMSKHKKSYKGGKEMLYRFEIFTDTLKYIEEKNKELKSYQLGLNQFADLKFDE 85
           ++F SWM KH K Y+   E +YRFEIF D L YI+E NK+  SY LGLN FADL  DE
Sbjct: 46  QLFDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKKNNSYWLGLNGFADLSNDE 103

The following BLAST results are available for this feature:

BLAST of Lag0014997 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_018829301.2	4.1e-12	53.95	cysteine protease XCP1-like [Juglans regia]	[more]
XP_038887495.1	4.1e-12	52.56	pro-cathepsin H-like [Benincasa hispida]	[more]
KAF5449130.1	4.1e-12	53.95	hypothetical protein F2P56_029607 [Juglans regia]	[more]
XP_041012569.1	2.0e-11	57.14	cysteine protease XCP1-like [Juglans microcarpa x Juglans regia]	[more]
KAG7978501.1	2.0e-11	57.14	hypothetical protein I3843_05G085500 [Carya illinoinensis]	[more]

BLAST of Lag0014997 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05994	1.7e-13	60.34	Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3	[more]
P00784	1.9e-12	60.34	Papain OS=Carica papaya OX=3649 PE=1 SV=1	[more]
O65493	5.6e-12	51.72	Cysteine protease XCP1 OS=Arabidopsis thaliana OX=3702 GN=XCP1 PE=1 SV=1	[more]
P10056	9.5e-12	58.62	Caricain OS=Carica papaya OX=3649 PE=1 SV=2	[more]
P14080	2.8e-11	58.62	Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 29..85 e-value: 1.5E-20 score: 84.3
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 29..84 e-value: 1.0E-16 score: 61.1
None	No IPR available	GENE3D	1.10.287.2250		coord: 9..90 e-value: 1.2E-21 score: 78.4
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 27..89
None	No IPR available	PANTHER	PTHR12411:SF719	SUBFAMILY NOT NAMED	coord: 27..89
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 23..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0014997.1	Lag0014997.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity