Lag0014921 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014921
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionXylem cysteine proteinase 1
Locationchr12: 5903816 .. 5904148 (+)
RNA-Seq ExpressionLag0014921
SyntenyLag0014921
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAATTGTTCAAGTCATGGATGTTGAAGAACGATAGGAGTTACGAGAGCGAGAAAGAGATGTTGTATAGGTATCAGATATTCTGTAAGAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTGTCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACAAGAACTCGGATGAGGTCCCGCGCCTGCGTCACCTCCATTTGGTACATCCCCGCGGAGGACGAGGACGAGGACGAGGCCTAGCTAATTGCAGCATTCATTCCAATTCTAATCCTTTTAATTTTATTATATTTGAACTATAG

mRNA sequence

ATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAATTGTTCAAGTCATGGATGTTGAAGAACGATAGGAGTTACGAGAGCGAGAAAGAGATGTTGTATAGGTATCAGATATTCTGTAAGAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTGTCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACAAGAACTCGGATGAGGTCCCGCGCCTGCGTCACCTCCATTTGGTACATCCCCGCGGAGGACGAGGACGAGGACGAGGCCTAGCTAATTGCAGCATTCATTCCAATTCTAATCCTTTTAATTTTATTATATTTGAACTATAG

Coding sequence (CDS)

ATGGAGGAAGCAGAAGGTTCGGAGAATTGGGAATTGTTCAAGTCATGGATGTTGAAGAACGATAGGAGTTACGAGAGCGAGAAAGAGATGTTGTATAGGTATCAGATATTCTGTAAGAGGGTGAAGATGGTTGATGAGTCGAACAAGAAGTGTCGTGGCCGTCCGACATTTGGGTTGACTTGCTTTGCAGACAAGAACTCGGATGAGGTCCCGCGCCTGCGTCACCTCCATTTGGTACATCCCCGCGGAGGACGAGGACGAGGACGAGGCCTAGCTAATTGCAGCATTCATTCCAATTCTAATCCTTTTAATTTTATTATATTTGAACTATAG

Protein sequence

MEEAEGSENWELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDEVPRLRHLHLVHPRGGRGRGRGLANCSIHSNSNPFNFIIFEL
Homology
BLAST of Lag0014921 vs. NCBI nr
Match: XP_038887495.1 (pro-cathepsin H-like [Benincasa hispida])

HSP 1 Score: 66.2 bits (160), Expect = 2.0e-07
Identity = 32/66 (48.48%), Postives = 45/66 (68.18%), Query Frame = 0

Query: 7   SENWELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKN 66
           SE+WE FKSWM  +++ Y SE+EMLYR+ +F K +K +++ NK   G  TFG   F+D  
Sbjct: 56  SEDWESFKSWMSMHNKKYGSEEEMLYRFGLFKKTLKRIEKLNKNYTG-CTFGFNHFSDLT 115

Query: 67  SDEVPR 73
            DEVP+
Sbjct: 116 FDEVPK 120

BLAST of Lag0014921 vs. NCBI nr
Match: KAE8650303.1 (hypothetical protein Csa_010836 [Cucumis sativus])

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-05
Identity = 30/73 (41.10%), Postives = 48/73 (65.75%), Query Frame = 0

Query: 1   MEEAEGSENWELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLT 60
           +E+AE S++W+ F SWM ++ + YES++E LYR+ IF   +K + + NK+  G  TFGL 
Sbjct: 153 LEDAERSKDWKKFASWMSEHKKKYESDEEKLYRFGIFRGELKHIKKLNKEDNG-CTFGLN 212

Query: 61  CFADKNSDEVPRL 74
            ++D  + E  RL
Sbjct: 213 QYSDLTNSEFNRL 224

BLAST of Lag0014921 vs. NCBI nr
Match: KAE8057439.1 (hypothetical protein FH972_014132 [Carpinus fangiana])

HSP 1 Score: 58.9 bits (141), Expect = 3.1e-05
Identity = 32/71 (45.07%), Postives = 46/71 (64.79%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDEV 70
           ELF+SWM K+ +SYES +E L+R+ IF   +K +DE+NKK       GL  FAD + +E 
Sbjct: 46  ELFESWMSKHSKSYESVEEKLHRFDIFKDNLKHIDETNKKV-SNYWLGLNEFADLSHEEF 105

Query: 71  PRLRHLHLVHP 82
            ++ HL L+ P
Sbjct: 106 KQM-HLGLIKP 114

BLAST of Lag0014921 vs. NCBI nr
Match: 3TNX_A (Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem resolution [Carica papaya] >3TNX_C Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem resolution [Carica papaya] >3USV_A Structure of the precursor of a thermostable variant of papain at 3.8 A resolution from a crystal soaked at pH 4 [Carica papaya] >3USV_C Structure of the precursor of a thermostable variant of papain at 3.8 A resolution from a crystal soaked at pH 4 [Carica papaya] >4QRX_A Crystal structure of pro-papain mutant at pH 4.0 [Carica papaya] >4QRX_C Crystal structure of pro-papain mutant at pH 4.0 [Carica papaya])

HSP 1 Score: 55.5 bits (132), Expect = 3.5e-04
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF+SWMLK+++ Y++  E +YR++IF   +K +DE+NKK       GL  FAD ++DE
Sbjct: 64  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKK-NNSYWLGLNVFADMSNDE 121

BLAST of Lag0014921 vs. NCBI nr
Match: 4QRG_A (Crystal structure of I86L mutant of papain [Carica papaya] >4QRG_B Crystal structure of I86L mutant of papain [Carica papaya])

HSP 1 Score: 55.5 bits (132), Expect = 3.5e-04
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF+SWMLK+++ Y++  E +YR++IF   +K +DE+NKK       GL  FAD ++DE
Sbjct: 64  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKK-NNSYWLGLNVFADMSNDE 121

BLAST of Lag0014921 vs. ExPASy Swiss-Prot
Match: P00784 (Papain OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 4.5e-07
Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF+SWMLK+++ Y++  E +YR++IF   +K +DE+NKK       GL  FAD ++DE
Sbjct: 46  QLFESWMLKHNKIYKNIDEKIYRFEIFKDNLKYIDETNKK-NNSYWLGLNVFADMSNDE 103

BLAST of Lag0014921 vs. ExPASy Swiss-Prot
Match: P14080 (Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-06
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF SWMLK+++ YES  E +YR++IF   +  +DE+NKK       GL  FAD ++DE
Sbjct: 46  QLFDSWMLKHNKIYESIDEKIYRFEIFRDNLMYIDETNKK-NNSYWLGLNGFADLSNDE 103

BLAST of Lag0014921 vs. ExPASy Swiss-Prot
Match: P05994 (Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-06
Identity = 26/59 (44.07%), Postives = 40/59 (67.80%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF SWMLK++++Y++  E LYR++IF   +K +DE NK   G    GL  F+D ++DE
Sbjct: 46  QLFNSWMLKHNKNYKNVDEKLYRFEIFKDNLKYIDERNKMINGY-WLGLNEFSDLSNDE 103

BLAST of Lag0014921 vs. ExPASy Swiss-Prot
Match: P10056 (Caricain OS=Carica papaya OX=3649 PE=1 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 3.8e-06
Identity = 26/59 (44.07%), Postives = 38/59 (64.41%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDE 70
           +LF SWML +++ YE+  E LYR++IF   +  +DE+NKK       GL  FAD ++DE
Sbjct: 46  QLFNSWMLNHNKFYENVDEKLYRFEIFKDNLNYIDETNKK-NNSYWLGLNEFADLSNDE 103

BLAST of Lag0014921 vs. ExPASy Swiss-Prot
Match: Q9LM66 (Cysteine protease XCP2 OS=Arabidopsis thaliana OX=3702 GN=XCP2 PE=1 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 4.7e-04
Identity = 21/63 (33.33%), Postives = 40/63 (63.49%), Query Frame = 0

Query: 11  ELFKSWMLKNDRSYESEKEMLYRYQIFCKRVKMVDESNKKCRGRPTFGLTCFADKNSDEV 70
           ELF++W+   +++YE+ +E   R+++F   +K +DE+NKK +     GL  FAD + +E 
Sbjct: 49  ELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSY-WLGLNEFADLSHEEF 108

Query: 71  PRL 74
            ++
Sbjct: 109 KKM 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887495.12.0e-0748.48pro-cathepsin H-like [Benincasa hispida][more]
KAE8650303.11.8e-0541.10hypothetical protein Csa_010836 [Cucumis sativus][more]
KAE8057439.13.1e-0545.07hypothetical protein FH972_014132 [Carpinus fangiana][more]
3TNX_A3.5e-0444.07Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem ... [more]
4QRG_A3.5e-0444.07Crystal structure of I86L mutant of papain [Carica papaya] >4QRG_B Crystal struc... [more]
Match NameE-valueIdentityDescription
P007844.5e-0744.07Papain OS=Carica papaya OX=3649 PE=1 SV=1[more]
P140801.3e-0645.76Chymopapain OS=Carica papaya OX=3649 PE=1 SV=2[more]
P059941.3e-0644.07Papaya proteinase 4 OS=Carica papaya OX=3649 PE=1 SV=3[more]
P100563.8e-0644.07Caricain OS=Carica papaya OX=3649 PE=1 SV=2[more]
Q9LM664.7e-0433.33Cysteine protease XCP2 OS=Arabidopsis thaliana OX=3702 GN=XCP2 PE=1 SV=2[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 13..70
e-value: 2.0E-10
score: 50.7
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 13..69
e-value: 1.6E-12
score: 47.7
NoneNo IPR availableGENE3D1.10.287.2250coord: 1..76
e-value: 1.3E-13
score: 52.7
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 8..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014921.1Lag0014921.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0110165 cellular anatomical entity
molecular_function GO:0008233 peptidase activity
molecular_function GO:0097655 serpin family protein binding