Lag0014896 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014896
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein E6-like
Locationchr12: 5642877 .. 5643629 (-)
RNA-Seq ExpressionLag0014896
SyntenyLag0014896
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATGGCAGCTTCCACCACTTTCAAGCATTTCTTCTTCTTCTTCCTCCTCCTCATTCTCTCCTCCGTCCAAATCGAAGCCCGAGTCAACAAATTCTTCAGCAAATTCATCCACACCGATCAGACTGTTGTTCTTCCGGTTCCCCCTTCCCTGGCGCCGGTCTCTGCTCCGCCGGAGATATCTCCTTCTCTGGCGCCGACGCCGGCTCCCGCTCCGTTTTTCGACGAATCGCAGAATGCTTACGGTCTGTACGGACGCGATTCCGACGACGGCGAGAGTAGCCGGACGATCACCGACGTGGAGGAGGAGATTCTCGCGGAGGACGGCGAGGAGAGCTACAAATCTGGCTATCCGAAGACGAATTTACACAGCGACAGTTTCGGAAGCCCTAGTAGATACGAGCAGTACCAGAGCAATTACGGGAACAATTACGAGAACAATGGATATAGAAATTCCGAGTACGAGAACAATCAGTACAGAAATTCGGAGTACGAGAGCAATTTTGAGAACAATGGCGGGAGAAATTACCAGTACCAGAGCAATTTCGAGGACAACGGCTACAGAAGGAGCCGATTCGAACCGACGGAGCGGCAAGGGATGAGCGACACTAGATTCATGGAGAACGGGAGGTACTATCACGACATTAACTCGAGGAATGGAGGAGAAGGCGAATCGTACGGAAGCAAGAAGAATCCCTACGAATTCGATTCGATGGAGGAGTATGAGAAGAGCGAGGGATTTCTTCCTTGA

mRNA sequence

ATGGCTATGGCAGCTTCCACCACTTTCAAGCATTTCTTCTTCTTCTTCCTCCTCCTCATTCTCTCCTCCGTCCAAATCGAAGCCCGAGTCAACAAATTCTTCAGCAAATTCATCCACACCGATCAGACTGTTGTTCTTCCGGTTCCCCCTTCCCTGGCGCCGGTCTCTGCTCCGCCGGAGATATCTCCTTCTCTGGCGCCGACGCCGGCTCCCGCTCCGTTTTTCGACGAATCGCAGAATGCTTACGGTCTGTACGGACGCGATTCCGACGACGGCGAGAGTAGCCGGACGATCACCGACGTGGAGGAGGAGATTCTCGCGGAGGACGGCGAGGAGAGCTACAAATCTGGCTATCCGAAGACGAATTTACACAGCGACAGTTTCGGAAGCCCTAGTAGATACGAGCAGTACCAGAGCAATTACGGGAACAATTACGAGAACAATGGATATAGAAATTCCGAGTACGAGAACAATCAGTACAGAAATTCGGAGTACGAGAGCAATTTTGAGAACAATGGCGGGAGAAATTACCAGTACCAGAGCAATTTCGAGGACAACGGCTACAGAAGGAGCCGATTCGAACCGACGGAGCGGCAAGGGATGAGCGACACTAGATTCATGGAGAACGGGAGGTACTATCACGACATTAACTCGAGGAATGGAGGAGAAGGCGAATCGTACGGAAGCAAGAAGAATCCCTACGAATTCGATTCGATGGAGGAGTATGAGAAGAGCGAGGGATTTCTTCCTTGA

Coding sequence (CDS)

ATGGCTATGGCAGCTTCCACCACTTTCAAGCATTTCTTCTTCTTCTTCCTCCTCCTCATTCTCTCCTCCGTCCAAATCGAAGCCCGAGTCAACAAATTCTTCAGCAAATTCATCCACACCGATCAGACTGTTGTTCTTCCGGTTCCCCCTTCCCTGGCGCCGGTCTCTGCTCCGCCGGAGATATCTCCTTCTCTGGCGCCGACGCCGGCTCCCGCTCCGTTTTTCGACGAATCGCAGAATGCTTACGGTCTGTACGGACGCGATTCCGACGACGGCGAGAGTAGCCGGACGATCACCGACGTGGAGGAGGAGATTCTCGCGGAGGACGGCGAGGAGAGCTACAAATCTGGCTATCCGAAGACGAATTTACACAGCGACAGTTTCGGAAGCCCTAGTAGATACGAGCAGTACCAGAGCAATTACGGGAACAATTACGAGAACAATGGATATAGAAATTCCGAGTACGAGAACAATCAGTACAGAAATTCGGAGTACGAGAGCAATTTTGAGAACAATGGCGGGAGAAATTACCAGTACCAGAGCAATTTCGAGGACAACGGCTACAGAAGGAGCCGATTCGAACCGACGGAGCGGCAAGGGATGAGCGACACTAGATTCATGGAGAACGGGAGGTACTATCACGACATTAACTCGAGGAATGGAGGAGAAGGCGAATCGTACGGAAGCAAGAAGAATCCCTACGAATTCGATTCGATGGAGGAGTATGAGAAGAGCGAGGGATTTCTTCCTTGA

Protein sequence

MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPEISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEESYKSGYPKTNLHSDSFGSPSRYEQYQSNYGNNYENNGYRNSEYENNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDINSRNGGEGESYGSKKNPYEFDSMEEYEKSEGFLP
Homology
BLAST of Lag0014896 vs. NCBI nr
Match: XP_022969172.1 (probable ATP-dependent RNA helicase ddx42 isoform X2 [Cucurbita maxima])

HSP 1 Score: 327.0 bits (837), Expect = 1.4e-85
Identity = 199/281 (70.82%), Postives = 220/281 (78.29%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F FLL  LSSVQIEARVNKFFSKFIH D+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIFLL--LSSVQIEARVNKFFSKFIHADRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEE--SYKSGY 120
           ISPSLAPTPAPAPFFDESQNAYGLYG D+DD ESSRTITDVEEEILAEDGE+  ++KSGY
Sbjct: 61  ISPSLAPTPAPAPFFDESQNAYGLYGSDADDSESSRTITDVEEEILAEDGEDKKTHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------YGNNYE--------NNGYR 180
            +TNLH+D+F SP RYE            +Y+SN       Y NN E        NN YR
Sbjct: 121 -QTNLHTDNFESPKRYESRNDGNSGYRNSEYESNNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 NSEYE--NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMEN 240
           NSEYE  NN+YRN+EY+S+FEN+G RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMEN
Sbjct: 181 NSEYENNNNEYRNTEYKSDFENSGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMEN 240

Query: 241 GRYYHDINSRNGGEGESYGSKKNPYEFDSMEEYEKSEGFLP 251
           GRYYH+INS  G E +SYGSKK P EFDSMEEYEKSEGFLP
Sbjct: 241 GRYYHEINSGIGEENKSYGSKKYPNEFDSMEEYEKSEGFLP 278

BLAST of Lag0014896 vs. NCBI nr
Match: XP_022969171.1 (protein E6-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 306.2 bits (783), Expect = 2.6e-79
Identity = 199/335 (59.40%), Postives = 220/335 (65.67%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F FLL  LSSVQIEARVNKFFSKFIH D+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIFLL--LSSVQIEARVNKFFSKFIHADRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEE--SYKSGY 120
           ISPSLAPTPAPAPFFDESQNAYGLYG D+DD ESSRTITDVEEEILAEDGE+  ++KSGY
Sbjct: 61  ISPSLAPTPAPAPFFDESQNAYGLYGSDADDSESSRTITDVEEEILAEDGEDKKTHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------YGNNYE--------NNGYR 180
            +TNLH+D+F SP RYE            +Y+SN       Y NN E        NN YR
Sbjct: 121 -QTNLHTDNFESPKRYESRNDGNSGYRNSEYESNNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 NSEYE------------------------------------------------------- 240
           NSEYE                                                       
Sbjct: 181 NSEYENDNEHRTSEDENNNEHRNSEYENNNEHKNSEYENNNEHRNSEYENNNEYRNSEHE 240

Query: 241 -NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHD 251
            NN+YRN+EY+S+FEN+G RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMENGRYYH+
Sbjct: 241 NNNEYRNTEYKSDFENSGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMENGRYYHE 300

BLAST of Lag0014896 vs. NCBI nr
Match: XP_038886989.1 (protein E6-like [Benincasa hispida])

HSP 1 Score: 305.1 bits (780), Expect = 5.7e-79
Identity = 182/256 (71.09%), Postives = 206/256 (80.47%), Query Frame = 0

Query: 4   AASTTFKHF-FFFFLLLILSSVQIEARVNKFFSKFIHTDQTVV-LPVPPSLAPVSAPPEI 63
           AASTTF    FFFF +L+LSSVQIEARVNKFFSKFI+TD+ VV   +PP  APVSAPPEI
Sbjct: 3   AASTTFNLLSFFFFFILLLSSVQIEARVNKFFSKFINTDREVVPTKLPP--APVSAPPEI 62

Query: 64  SPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDG----EESYKSG 123
           SPSLAPTPAPAPFFDESQNAYGLYGRD+D  E++RTITDVEEEILA DG    E+++K+ 
Sbjct: 63  SPSLAPTPAPAPFFDESQNAYGLYGRDADADENTRTITDVEEEILAGDGEDEDEDNHKAA 122

Query: 124 YPKTNLHSDSFGSPSRYEQYQSNYGNNYE-NNGYRNSEYEN-NQYRNSEYESNFENNGGR 183
           YP TN  + ++G+            NNYE NNG+RNSEYEN N+YRNSEYES FENN  R
Sbjct: 123 YPMTNSQTGNYGN------------NNYENNNGFRNSEYENHNEYRNSEYESAFENNNAR 182

Query: 184 NYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDINSRNGGEGESYGSKKNP-Y 243
           NYQYQSNFED+GYRR R EPT +QGMSDTRFMENGRY+HDINS+NG E  SYG+ K P Y
Sbjct: 183 NYQYQSNFEDDGYRRRRHEPTRQQGMSDTRFMENGRYFHDINSKNGEENGSYGNNKYPKY 242

Query: 244 EFDSMEEYEKSEGFLP 251
           EFDSMEEYE+SEG LP
Sbjct: 243 EFDSMEEYERSEGLLP 244

BLAST of Lag0014896 vs. NCBI nr
Match: XP_023554545.1 (GATA zinc finger domain-containing protein 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 303.1 bits (775), Expect = 2.2e-78
Identity = 200/336 (59.52%), Postives = 217/336 (64.58%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F F   ILSSVQIEARVNKFFSKFIHTD+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIF--FILSSVQIEARVNKFFSKFIHTDRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDG--EESYKSGY 120
           ISPSLAPT APAPFFDESQNAYGLYG D+DD E SRTITDVEEEILAEDG  EES+KSGY
Sbjct: 61  ISPSLAPTLAPAPFFDESQNAYGLYGSDADDSEGSRTITDVEEEILAEDGEDEESHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------------------------- 180
            +TNLHSD+F SP R E            +Y+SN                          
Sbjct: 121 -QTNLHSDNFESPKRSESNNDGNSGYRNSEYESNNVHRNSEFENNNEHRNSEYENNNEYR 180

Query: 181 ------------------------------------YGNNYE--------NNGYRNSEYE 240
                                               Y NN E        NN YRNSEYE
Sbjct: 181 NSEHENNNEHRTSEDENNNEHRNSEYENNNEHKNSEYENNNEHRNSEYENNNEYRNSEYE 240

Query: 241 --NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYH 251
             NN+YRN+EY+S+FENNG RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMENGRYYH
Sbjct: 241 NNNNEYRNTEYKSDFENNGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMENGRYYH 300

BLAST of Lag0014896 vs. NCBI nr
Match: KAG6572262.1 (hypothetical protein SDJN03_28990, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 300.8 bits (769), Expect = 1.1e-77
Identity = 198/336 (58.93%), Postives = 217/336 (64.58%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F F  LILSSVQIEARVNKFFSKFIHTD+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIF--LILSSVQIEARVNKFFSKFIHTDRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDG--EESYKSGY 120
           ISPSLAPT APAPFFDESQNAYGLYG D+DD E SRTITDVEEEIL EDG  EES+KSGY
Sbjct: 61  ISPSLAPTLAPAPFFDESQNAYGLYGSDADDSEGSRTITDVEEEILTEDGEDEESHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------------------------------------- 180
            +TNLH+D+F SP R E                                           
Sbjct: 121 -QTNLHTDNFESPKRSESNNHGNSGYRNSEYENDNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 ------------------------QYQSN-------YGNNYE--------NNGYRNSEYE 240
                                   +Y++N       Y NN E        NN YRNSEYE
Sbjct: 181 NSEYENNNEHGTSEDENNNEHRTSEYENNNEHKKSEYENNNEHRNSEYENNNEYRNSEYE 240

Query: 241 --NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYH 251
             NN+YRN+EY+S+FENNG RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMENGRYYH
Sbjct: 241 NNNNEYRNTEYKSDFENNGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMENGRYYH 300

BLAST of Lag0014896 vs. ExPASy TrEMBL
Match: A0A6J1HVL6 (probable ATP-dependent RNA helicase ddx42 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111468248 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 6.8e-86
Identity = 199/281 (70.82%), Postives = 220/281 (78.29%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F FLL  LSSVQIEARVNKFFSKFIH D+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIFLL--LSSVQIEARVNKFFSKFIHADRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEE--SYKSGY 120
           ISPSLAPTPAPAPFFDESQNAYGLYG D+DD ESSRTITDVEEEILAEDGE+  ++KSGY
Sbjct: 61  ISPSLAPTPAPAPFFDESQNAYGLYGSDADDSESSRTITDVEEEILAEDGEDKKTHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------YGNNYE--------NNGYR 180
            +TNLH+D+F SP RYE            +Y+SN       Y NN E        NN YR
Sbjct: 121 -QTNLHTDNFESPKRYESRNDGNSGYRNSEYESNNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 NSEYE--NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMEN 240
           NSEYE  NN+YRN+EY+S+FEN+G RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMEN
Sbjct: 181 NSEYENNNNEYRNTEYKSDFENSGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMEN 240

Query: 241 GRYYHDINSRNGGEGESYGSKKNPYEFDSMEEYEKSEGFLP 251
           GRYYH+INS  G E +SYGSKK P EFDSMEEYEKSEGFLP
Sbjct: 241 GRYYHEINSGIGEENKSYGSKKYPNEFDSMEEYEKSEGFLP 278

BLAST of Lag0014896 vs. ExPASy TrEMBL
Match: A0A6J1HX03 (protein E6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468248 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.2e-79
Identity = 199/335 (59.40%), Postives = 220/335 (65.67%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F FLL  LSSVQIEARVNKFFSKFIH D+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIFLL--LSSVQIEARVNKFFSKFIHADRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEE--SYKSGY 120
           ISPSLAPTPAPAPFFDESQNAYGLYG D+DD ESSRTITDVEEEILAEDGE+  ++KSGY
Sbjct: 61  ISPSLAPTPAPAPFFDESQNAYGLYGSDADDSESSRTITDVEEEILAEDGEDKKTHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------YGNNYE--------NNGYR 180
            +TNLH+D+F SP RYE            +Y+SN       Y NN E        NN YR
Sbjct: 121 -QTNLHTDNFESPKRYESRNDGNSGYRNSEYESNNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 NSEYE------------------------------------------------------- 240
           NSEYE                                                       
Sbjct: 181 NSEYENDNEHRTSEDENNNEHRNSEYENNNEHKNSEYENNNEHRNSEYENNNEYRNSEHE 240

Query: 241 -NNQYRNSEYESNFENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHD 251
            NN+YRN+EY+S+FEN+G RNYQYQSN E +GYR+ R+EPTE+QGMSDTRFMENGRYYH+
Sbjct: 241 NNNEYRNTEYKSDFENSGVRNYQYQSNVEGDGYRKRRYEPTEQQGMSDTRFMENGRYYHE 300

BLAST of Lag0014896 vs. ExPASy TrEMBL
Match: A0A6J1GLC8 (GATA zinc finger domain-containing protein 14-like OS=Cucurbita moschata OX=3662 GN=LOC111455418 PE=4 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.7e-74
Identity = 200/380 (52.63%), Postives = 219/380 (57.63%), Query Frame = 0

Query: 1   MAMAASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPE 60
           MAMAAS TFKH  F F  LILSSVQIEARVNKFFSKFIHTD+ VVLPV  S APVS PPE
Sbjct: 1   MAMAASITFKHLPFIF--LILSSVQIEARVNKFFSKFIHTDRDVVLPVAFSPAPVSVPPE 60

Query: 61  ISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDG--EESYKSGY 120
           ISPSLAPTPAPAPFFDESQNAYGLYG D+D+ E SRTITDVEEEILAEDG  EES+KSGY
Sbjct: 61  ISPSLAPTPAPAPFFDESQNAYGLYGSDADNSEGSRTITDVEEEILAEDGEDEESHKSGY 120

Query: 121 PKTNLHSDSFGSPSRYE------------QYQSN-------------------------- 180
            +TNLH+D+F SP R E            +Y+SN                          
Sbjct: 121 -QTNLHTDNFESPKRSESNNDGNSGYRNSEYESNNDHRNSEYENNNEHRNSEYENNNEYR 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 NSEYENNNEHRTSEDENNNEHRNSEYENNNEHRTSEDENNNEHRNSEYENNNEHRTSEDE 240

Query: 241 --------------------YGNNYE--------NNGYRNSEYE--NNQYRNSEYESNFE 251
                               Y NN E        NN YRNSEYE  NN+YRN+EY+S+FE
Sbjct: 241 NNNEHRNSEYENNNEHKNSEYENNNEHRNSEYENNNEYRNSEYENNNNEYRNTEYKSDFE 300

BLAST of Lag0014896 vs. ExPASy TrEMBL
Match: A0A6J1C3G6 (protein E6-like OS=Momordica charantia OX=3673 GN=LOC111007909 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-73
Identity = 174/255 (68.24%), Postives = 192/255 (75.29%), Query Frame = 0

Query: 6   STTFKHF-FFFFLLLILSSVQIEARVNKFFSKFIHTDQ------TVVLPVPPSLAPVSAP 65
           ++  KH  FFF LLL+LSSVQIEARVNKFFSKFIHTD+      T  LPV PS AP+SAP
Sbjct: 2   ASALKHLPFFFLLLLLLSSVQIEARVNKFFSKFIHTDRNDASPLTPALPVAPSPAPLSAP 61

Query: 66  PEISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAED-GEESYKSG 125
           PEISP LAPT    PFF ESQNAYGLYGR SDD E++ +ITDVEEEILAED G+ESYKSG
Sbjct: 62  PEISPILAPT----PFFYESQNAYGLYGRASDDTENNPSITDVEEEILAEDGGDESYKSG 121

Query: 126 YPKTNLHSDSFGSPSRYEQYQSNYGNNYENNGYRNSEYENNQYRNSEYESNFENNGGRNY 185
           YPKT+ H   F S  R EQYQS+YG    NNGY NSEY              ENNGGRNY
Sbjct: 122 YPKTSFHGTDFESSRRDEQYQSSYG----NNGYGNSEY--------------ENNGGRNY 181

Query: 186 QYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDINSRNGGEGESYGSKKNP---- 245
           QY+SNFED G+RRSR+EP ERQGMSDTRF+ENG+YY+D NSR G  GESYGSKKNP    
Sbjct: 182 QYESNFEDGGFRRSRYEPRERQGMSDTRFVENGKYYYDANSRAGEGGESYGSKKNPIPNQ 234

Query: 246 YEFDSMEEYEKSEGF 249
           +EFDSMEEYEKSE F
Sbjct: 242 FEFDSMEEYEKSEEF 234

BLAST of Lag0014896 vs. ExPASy TrEMBL
Match: A0A5D3DDN1 (Protein E6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00230 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.3e-73
Identity = 180/258 (69.77%), Postives = 196/258 (75.97%), Query Frame = 0

Query: 2   AMAASTT--FKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPP 61
           A +ASTT  F H  FFFLLL LSSVQ EARVNKFFSKFIHTD   V+P   S AP+S PP
Sbjct: 3   AASASTTFNFNHLSFFFLLL-LSSVQTEARVNKFFSKFIHTDHEEVVPNTLSPAPLSVPP 62

Query: 62  EISPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDG---EESYKS 121
           E SPSLAPTPAPAPFFDESQNAYGLYG D D  E+ RTITDVEEEIL  +G   E + KS
Sbjct: 63  ETSPSLAPTPAPAPFFDESQNAYGLYGSDPDTDENPRTITDVEEEILGGEGDQDETNRKS 122

Query: 122 GYPKTNLHSDSFGSPSRYEQYQSNYGNNYE-NNGYRNSEYEN-NQYRNSEYESNFENNGG 181
            +P  N       +    EQYQ+    NYE NNG+RNSEYEN N+YRNSEYE+N  NN G
Sbjct: 123 EFPMNNF----VQTRDDEEQYQN---KNYEYNNGFRNSEYENHNEYRNSEYENN--NNEG 182

Query: 182 RNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDINSRNGGEGESYGSKKN-- 241
           RNYQYQSNFED GYRRSRFEPTE+QGMSDTRFMENGRY+HDINS+N  E  SYGSKK   
Sbjct: 183 RNYQYQSNFEDGGYRRSRFEPTEQQGMSDTRFMENGRYFHDINSKNDEENGSYGSKKKYP 242

Query: 242 PYEFDSMEEYEKSEGFLP 251
            YEFDSMEEYE+SEG LP
Sbjct: 243 KYEFDSMEEYERSEGLLP 250

BLAST of Lag0014896 vs. TAIR 10
Match: AT2G33850.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G28400.1); Has 3053 Blast hits to 2119 proteins in 133 species: Archae - 6; Bacteria - 52; Metazoa - 135; Fungi - 96; Plants - 73; Viruses - 2; Other Eukaryotes - 2689 (source: NCBI BLink). )

HSP 1 Score: 58.9 bits (141), Expect = 6.6e-09
Identity = 76/268 (28.36%), Postives = 113/268 (42.16%), Query Frame = 0

Query: 5   ASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIHTDQTVVLPVPPSLAPVSAPPEISPS 64
           A +T    FFF L L+L S QI AR +  F KF   D       P +L P+    E +  
Sbjct: 2   AFSTSSCLFFFLLTLVLFSTQISARNSYSFGKFQREDPKE--QNPNNLVPI----ETNEK 61

Query: 65  LAPTPAPAPFFDESQNAYGLYGRDSDDG----------------ESSRTITDVEEEILAE 124
             P      F  +S+N YGLYG ++ D                 + S +   + E    +
Sbjct: 62  KEPDDQNPAFIPQSENGYGLYGHETTDNNNEELNNNKYEDNVNYDDSFSTPSLSETAQTQ 121

Query: 125 DGEESYKSGYPKTNLHSDSFGSPSRYEQYQSNYGNNYENNGYRNSEYENNQYRNSEYESN 184
           +  ++YK  YPKT    D+    S YE   + YG +  +N   N  Y+    +++ Y  N
Sbjct: 122 ESYKNYKESYPKTTEIYDNNKDTSYYEN-SNAYGTDKRDNDI-NDPYKGYSNKDTSYYEN 181

Query: 185 FENNGGRNYQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDI------------ 242
               G    + +  +   GY  +     ERQGMSDTR+M NG+YY+D+            
Sbjct: 182 PNTYGTEKREKEPAY--RGYNNN----VERQGMSDTRYMANGKYYYDLDDDRNHGRFYQN 241

BLAST of Lag0014896 vs. TAIR 10
Match: AT1G03820.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; Has 1345 Blast hits to 1122 proteins in 102 species: Archae - 2; Bacteria - 28; Metazoa - 28; Fungi - 30; Plants - 109; Viruses - 0; Other Eukaryotes - 1148 (source: NCBI BLink). )

HSP 1 Score: 55.5 bits (132), Expect = 7.3e-08
Identity = 74/254 (29.13%), Postives = 98/254 (38.58%), Query Frame = 0

Query: 5   ASTTFKHFFFFFLLLILSSVQIEARVNK-FFSKFIHTDQTVVLPVPPSLAPVSAPPEISP 64
           AS   K  F F  +       +EAR  K FFSKF H D+               P     
Sbjct: 2   ASFALKPIFCFIAVFCFIVHNVEAREGKLFFSKFTHIDR---------------PNNKDV 61

Query: 65  SLAPTPAPAPFFDESQNAYGLYGRDS-------DDGESSRTITDVEEEILAEDGEESYKS 124
           +L+P PAP       +   G +G  S       +   SS T TD E E L    +E   +
Sbjct: 62  ALSPAPAPGLAQANGRLGNGSFGPGSGMIPQTKESWPSSSTTTDEEFEKLMATFDEEKNT 121

Query: 125 GYPKTNLHSDSFGSPSRYEQYQSNYGNNYENNGYRNSEYENNQYRNSEYESNFENNGGRN 184
             P+     +         + +  Y NN  NNGY              Y +N  N+ GR 
Sbjct: 122 KLPEAFEEEEESEDSEDLNEPKDKYNNNNNNNGY-------------TYTTNNYNDNGRG 181

Query: 185 YQYQSNFEDNGYRRSRFEPTERQGMSDTRFMENGRYYHDINSRNGGEGESYG-----SKK 244
           Y  +                E+QGMSDTR MENG+Y++D   RN     S G        
Sbjct: 182 YGNEE---------------EKQGMSDTRVMENGKYFYDTRGRNSENTPSRGYENARGND 212

Query: 245 NPYEFDSMEEYEKS 246
           +  EF++MEEY KS
Sbjct: 242 HTNEFETMEEYYKS 212

BLAST of Lag0014896 vs. TAIR 10
Match: AT1G28400.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G33850.1); Has 45374 Blast hits to 18870 proteins in 668 species: Archae - 72; Bacteria - 1460; Metazoa - 1191; Fungi - 1038; Plants - 174; Viruses - 64; Other Eukaryotes - 41375 (source: NCBI BLink). )

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-07
Identity = 83/286 (29.02%), Postives = 114/286 (39.86%), Query Frame = 0

Query: 5   ASTTFKHFFFFFLLLILSSVQIEARVNKFFSKFIH---TDQTVVLPVPPSLAPVSAPPEI 64
           A +T     FFF  L+L S QI AR + FF KF      DQ     +P   +  +   E 
Sbjct: 2   AFSTRGSLLFFFTTLVLLSTQIHARDSYFFGKFHRESPKDQNPNSFIPLETSEKTTVEES 61

Query: 65  SPSLAPTPAPAPFFDESQNAYGLYGRDSDDGESSRTITDVEEEILAEDGEESYKSGYPKT 124
             +         F  ES N YGLYG ++    ++            ++ EE   +     
Sbjct: 62  VLNKKEQEQDPTFVPESGNGYGLYGHETTYNNNN------------DNKEEFNNNNKNDE 121

Query: 125 NLHSDSFGSPS------RYEQYQSNY---GNNYENNGYRNSEYENN----------QYRN 184
            ++S +F +PS       +  Y+ NY     NY   GY N E+ NN          ++ N
Sbjct: 122 KVNSKTFSTPSLSETEESFNNYEENYPKKTENYGTKGYNNEEFNNNNNKYDANFKEEFNN 181

Query: 185 SEYESNF------ENNGGRNYQYQ-----------SNFEDN----------GYRRSRFEP 234
           ++Y+ N+       NN   NY Y+            N EDN          G    R  P
Sbjct: 182 NKYDENYAKEEFNNNNNNNNYNYKYDENVKEESFPENNEDNKKNVYNSNAYGTELERETP 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022969172.11.4e-8570.82probable ATP-dependent RNA helicase ddx42 isoform X2 [Cucurbita maxima][more]
XP_022969171.12.6e-7959.40protein E6-like isoform X1 [Cucurbita maxima][more]
XP_038886989.15.7e-7971.09protein E6-like [Benincasa hispida][more]
XP_023554545.12.2e-7859.52GATA zinc finger domain-containing protein 14-like [Cucurbita pepo subsp. pepo][more]
KAG6572262.11.1e-7758.93hypothetical protein SDJN03_28990, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1HVL66.8e-8670.82probable ATP-dependent RNA helicase ddx42 isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1HX031.2e-7959.40protein E6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468248 PE=4 SV=1[more]
A0A6J1GLC82.7e-7452.63GATA zinc finger domain-containing protein 14-like OS=Cucurbita moschata OX=3662... [more]
A0A6J1C3G61.0e-7368.24protein E6-like OS=Momordica charantia OX=3673 GN=LOC111007909 PE=4 SV=1[more]
A0A5D3DDN12.3e-7369.77Protein E6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G0023... [more]
Match NameE-valueIdentityDescription
AT2G33850.16.6e-0928.36unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G03820.17.3e-0829.13unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT1G28400.11.6e-0729.02unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..154
NoneNo IPR availablePANTHERPTHR35274:SF5BNAC05G02100D PROTEINcoord: 3..245
IPR040290Protein E6-likePANTHERPTHR35274E6-LIKE PROTEINcoord: 3..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014896.1Lag0014896.1mRNA