Lag0014884 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014884
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr12: 5564995 .. 5565408 (+)
RNA-Seq ExpressionLag0014884
SyntenyLag0014884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTAGAACTTTTGGATGGCGCCACAATTATCAACTTTGTCGAAGATGAAGAAGCTTTTGGTACGACAATTAGAGAGCGTTTCAATCACCTCGACACCAATAGAGATGGTGTTCTCTCTTATGGAGAGATGTTGAGGGAGTTGCAAAGTCTTAGAGTGTTCGAGACTCATTTCGGGATCGATGTGAAACCCGACCCGGACGAGCTCTCTTCGGTCTACGGCTCGTTGTTCTTGCAATTTGATCGGGACTGCAATGGAAAAATTGACCTAGATGAGTTCATGGCGGAAACTAAGAGGATGATGTTGGCCATGGCGAATGGGATTGGATTTTTGCCTATTCAAATGGTGTTGGAAGAAAACAGCTTCTTGAAGAAAGCTGTGGAGAGAGAATCCACTAGAGTGGCTGCTTAA

mRNA sequence

ATGAGCGTAGAACTTTTGGATGGCGCCACAATTATCAACTTTGTCGAAGATGAAGAAGCTTTTGGTACGACAATTAGAGAGCGTTTCAATCACCTCGACACCAATAGAGATGGTGTTCTCTCTTATGGAGAGATGTTGAGGGAGTTGCAAAGTCTTAGAGTGTTCGAGACTCATTTCGGGATCGATGTGAAACCCGACCCGGACGAGCTCTCTTCGGTCTACGGCTCGTTGTTCTTGCAATTTGATCGGGACTGCAATGGAAAAATTGACCTAGATGAGTTCATGGCGGAAACTAAGAGGATGATGTTGGCCATGGCGAATGGGATTGGATTTTTGCCTATTCAAATGGTGTTGGAAGAAAACAGCTTCTTGAAGAAAGCTGTGGAGAGAGAATCCACTAGAGTGGCTGCTTAA

Coding sequence (CDS)

ATGAGCGTAGAACTTTTGGATGGCGCCACAATTATCAACTTTGTCGAAGATGAAGAAGCTTTTGGTACGACAATTAGAGAGCGTTTCAATCACCTCGACACCAATAGAGATGGTGTTCTCTCTTATGGAGAGATGTTGAGGGAGTTGCAAAGTCTTAGAGTGTTCGAGACTCATTTCGGGATCGATGTGAAACCCGACCCGGACGAGCTCTCTTCGGTCTACGGCTCGTTGTTCTTGCAATTTGATCGGGACTGCAATGGAAAAATTGACCTAGATGAGTTCATGGCGGAAACTAAGAGGATGATGTTGGCCATGGCGAATGGGATTGGATTTTTGCCTATTCAAATGGTGTTGGAAGAAAACAGCTTCTTGAAGAAAGCTGTGGAGAGAGAATCCACTAGAGTGGCTGCTTAA

Protein sequence

MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFGIDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEENSFLKKAVERESTRVAA
Homology
BLAST of Lag0014884 vs. NCBI nr
Match: XP_022136066.1 (uncharacterized protein LOC111007849 [Momordica charantia])

HSP 1 Score: 243.0 bits (619), Expect = 1.5e-60
Identity = 116/137 (84.67%), Postives = 130/137 (94.89%), Query Frame = 0

Query: 1   MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFG 60
           MSVELLDGATI++FVEDEE FG T++ERF+HLD++RDGVLSYGEMLRELQSLRV ETHFG
Sbjct: 1   MSVELLDGATIVSFVEDEEVFGATVKERFSHLDSDRDGVLSYGEMLRELQSLRVLETHFG 60

Query: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEE 120
           IDVKPDPDE+S+VYGSLFL FDRDCNGK+DL EFMAETK+MMLAMANGIG LPIQMVLEE
Sbjct: 61  IDVKPDPDEVSAVYGSLFLLFDRDCNGKVDLGEFMAETKKMMLAMANGIGLLPIQMVLEE 120

Query: 121 NSFLKKAVERESTRVAA 138
           N FLKKAVERE+T++AA
Sbjct: 121 NGFLKKAVERETTKLAA 137

BLAST of Lag0014884 vs. NCBI nr
Match: XP_038888629.1 (uncharacterized protein LOC120078427 [Benincasa hispida])

HSP 1 Score: 238.0 bits (606), Expect = 4.7e-59
Identity = 114/137 (83.21%), Postives = 127/137 (92.70%), Query Frame = 0

Query: 1   MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFG 60
           MSVE+LD ATI+NFVEDEEAF  +IRERF+HLD +RDGVL YGEML+ELQSLRV ETHFG
Sbjct: 1   MSVEILDSATIVNFVEDEEAFSDSIRERFSHLDLDRDGVLCYGEMLKELQSLRVLETHFG 60

Query: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEE 120
           ID KPDPDELSSVYGSLF QFDRDCNGK+DL EFMAETK+MMLAMANGIGF P+QM+LEE
Sbjct: 61  IDTKPDPDELSSVYGSLFSQFDRDCNGKVDLGEFMAETKKMMLAMANGIGFSPVQMLLEE 120

Query: 121 NSFLKKAVERESTRVAA 138
           NSFLKKAV+REST++AA
Sbjct: 121 NSFLKKAVDRESTKLAA 137

BLAST of Lag0014884 vs. NCBI nr
Match: XP_023529818.1 (uncharacterized protein LOC111792532 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.0 bits (606), Expect = 4.7e-59
Identity = 114/137 (83.21%), Postives = 126/137 (91.97%), Query Frame = 0

Query: 1   MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFG 60
           MSVE+LDGATI+NFVED+E FG  +RERF+HLD + DGVLSY EMLRELQSLRVFETHFG
Sbjct: 1   MSVEILDGATIVNFVEDDEVFGAIVRERFSHLDIDGDGVLSYEEMLRELQSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEE 120
           IDVKPDPDELSSVYGSLFLQFDRDCNGK+D+ EFM ETK+MM+AMANGIG LP+QMVLEE
Sbjct: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKVDVGEFMEETKKMMVAMANGIGLLPVQMVLEE 120

Query: 121 NSFLKKAVERESTRVAA 138
           NS LKKAVEREST+V A
Sbjct: 121 NSLLKKAVERESTKVGA 137

BLAST of Lag0014884 vs. NCBI nr
Match: KAG6589130.1 (hypothetical protein SDJN03_17695, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.9 bits (603), Expect = 1.0e-58
Identity = 113/137 (82.48%), Postives = 126/137 (91.97%), Query Frame = 0

Query: 1   MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFG 60
           MSVE+LDGATI+NFVED+E FG  +RERF+HLD + DGVLSY EMLRELQSLRVFETHFG
Sbjct: 1   MSVEILDGATIVNFVEDDEVFGAIVRERFSHLDIDGDGVLSYEEMLRELQSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEE 120
           IDVKPDPDELSSVYGSLFLQFDRDCNGK+D+ EFM ETK+MM+AMANGIGF P+QMVLEE
Sbjct: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKVDVGEFMEETKKMMVAMANGIGFSPVQMVLEE 120

Query: 121 NSFLKKAVERESTRVAA 138
           NS LK+AVEREST+V A
Sbjct: 121 NSLLKRAVERESTKVGA 137

BLAST of Lag0014884 vs. NCBI nr
Match: XP_022930660.1 (uncharacterized protein LOC111437061 [Cucurbita moschata])

HSP 1 Score: 236.1 bits (601), Expect = 1.8e-58
Identity = 113/137 (82.48%), Postives = 126/137 (91.97%), Query Frame = 0

Query: 1   MSVELLDGATIINFVEDEEAFGTTIRERFNHLDTNRDGVLSYGEMLRELQSLRVFETHFG 60
           MSVE+LD ATI+NFVED+E FG  +RERF+HLD + DGVLSY EMLRELQSLRVFETHFG
Sbjct: 1   MSVEILDSATIVNFVEDDEVFGAIVRERFSHLDIDGDGVLSYEEMLRELQSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYGSLFLQFDRDCNGKIDLDEFMAETKRMMLAMANGIGFLPIQMVLEE 120
           IDVKPDPDELSSVYGSLFLQFDRDC+GK+D+ EFM ETK+MM+AMANGIGF P+QMVLEE
Sbjct: 61  IDVKPDPDELSSVYGSLFLQFDRDCDGKVDVGEFMEETKKMMVAMANGIGFSPVQMVLEE 120

Query: 121 NSFLKKAVERESTRVAA 138
           NSFLKKAVEREST+V A
Sbjct: 121 NSFLKKAVERESTKVGA 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136066.11.5e-6084.67uncharacterized protein LOC111007849 [Momordica charantia][more]
XP_038888629.14.7e-5983.21uncharacterized protein LOC120078427 [Benincasa hispida][more]
XP_023529818.14.7e-5983.21uncharacterized protein LOC111792532 [Cucurbita pepo subsp. pepo][more]
KAG6589130.11.0e-5882.48hypothetical protein SDJN03_17695, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022930660.11.8e-5882.48uncharacterized protein LOC111437061 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 73..101
e-value: 2.7
score: 14.2
coord: 24..52
e-value: 0.18
score: 20.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 24..96
e-value: 5.7E-12
score: 45.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 69..104
score: 9.854874
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 9.101698
NoneNo IPR availableGENE3D1.10.238.10coord: 17..104
e-value: 5.4E-12
score: 47.7
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 1..133
NoneNo IPR availablePANTHERPTHR34574:SF10CALCIUM-BINDING EF-HAND FAMILY PROTEIN-LIKEcoord: 1..133
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 82..94
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 25..101

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014884.1Lag0014884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding