Lag0014785 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0014785 vs. NCBI nr
Match: KAG6572211.1 (putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011849.1 putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 294.7 bits (753), Expect = 4.7e-76
Identity = 138/154 (89.61%), Postives = 148/154 (96.10%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRLSSPSSLVPSSDSLENSS 60
           MGLSSLPAPSEGV+NA+L+N ALSIS+FKCIVRLILHIVGIR SSPSSLVPS+DS ENSS
Sbjct: 1   MGLSSLPAPSEGVLNAILINAALSISIFKCIVRLILHIVGIRFSSPSSLVPSTDSFENSS 60

Query: 61  ELGDSNFGSSWSYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHL 120
           ELGDSNFGSSWSY+EMFRNRY +IRFD+VQGSE  EHDCSVCLTQFEPESEINHLSCGHL
Sbjct: 61  ELGDSNFGSSWSYLEMFRNRYPSIRFDKVQGSERLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHSECLEKWLDYWNVTCPLCRTPLMSEEEKSCFW 155
           FH+ECLEKWLDYWN+TCPLCRTPLMSEEEKSCFW
Sbjct: 121 FHTECLEKWLDYWNITCPLCRTPLMSEEEKSCFW 154

BLAST of Lag0014785 vs. NCBI nr
Match: XP_022969282.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022969284.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima])

HSP 1 Score: 294.3 bits (752), Expect = 6.2e-76
Identity = 138/154 (89.61%), Postives = 147/154 (95.45%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRLSSPSSLVPSSDSLENSS 60
           MGLSSLPAPSEGV+NA+L+N ALSIS+FKCIVRLILHIVGIR SSPSSLVPS+DS ENSS
Sbjct: 1   MGLSSLPAPSEGVLNAILINAALSISIFKCIVRLILHIVGIRFSSPSSLVPSTDSFENSS 60

Query: 61  ELGDSNFGSSWSYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHL 120
           ELGDSNFGSSWSY+EMFRNRY  IRFD+VQGSE  EHDCSVCLTQFEPESEINHLSCGHL
Sbjct: 61  ELGDSNFGSSWSYLEMFRNRYPNIRFDKVQGSERLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHSECLEKWLDYWNVTCPLCRTPLMSEEEKSCFW 155
           FH+ECLEKWLDYWN+TCPLCRTPLMSEEEKSCFW
Sbjct: 121 FHTECLEKWLDYWNITCPLCRTPLMSEEEKSCFW 154

BLAST of Lag0014785 vs. NCBI nr
Match: XP_023554631.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo])

HSP 1 Score: 293.5 bits (750), Expect = 1.1e-75
Identity = 137/154 (88.96%), Postives = 148/154 (96.10%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRLSSPSSLVPSSDSLENSS 60
           MGLSSLPAPSEGV+NA+L+N ALSIS+FKCIVRLILHI+GIR SSPSSLVPS+DS ENSS
Sbjct: 1   MGLSSLPAPSEGVLNAILINAALSISIFKCIVRLILHILGIRFSSPSSLVPSTDSFENSS 60

Query: 61  ELGDSNFGSSWSYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHL 120
           ELGDSNFGSSWSY+EMFRNRY +IRFD+VQGSE  EHDCSVCLTQFEPESEINHLSCGHL
Sbjct: 61  ELGDSNFGSSWSYLEMFRNRYPSIRFDKVQGSERLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHSECLEKWLDYWNVTCPLCRTPLMSEEEKSCFW 155
           FH+ECLEKWLDYWN+TCPLCRTPLMSEEEKSCFW
Sbjct: 121 FHTECLEKWLDYWNITCPLCRTPLMSEEEKSCFW 154

BLAST of Lag0014785 vs. NCBI nr
Match: XP_022952716.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022952717.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022952718.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata])

HSP 1 Score: 292.4 bits (747), Expect = 2.4e-75
Identity = 136/154 (88.31%), Postives = 148/154 (96.10%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRLSSPSSLVPSSDSLENSS 60
           MGLSSLPAPSEGV+NA+L+N ALSIS+F+CIVRLILHI+GIR SSPSSLVPS+DS ENSS
Sbjct: 1   MGLSSLPAPSEGVLNAILINAALSISIFRCIVRLILHILGIRFSSPSSLVPSTDSFENSS 60

Query: 61  ELGDSNFGSSWSYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHL 120
           ELGDSNFGSSWSY+EMFRNRY +IRFD+VQGSE  EHDCSVCLTQFEPESEINHLSCGHL
Sbjct: 61  ELGDSNFGSSWSYLEMFRNRYPSIRFDKVQGSERLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHSECLEKWLDYWNVTCPLCRTPLMSEEEKSCFW 155
           FH+ECLEKWLDYWN+TCPLCRTPLMSEEEKSCFW
Sbjct: 121 FHTECLEKWLDYWNITCPLCRTPLMSEEEKSCFW 154

BLAST of Lag0014785 vs. NCBI nr
Match: XP_038887530.1 (probable E3 ubiquitin-protein ligase XERICO isoform X1 [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 9.9e-74
Identity = 135/154 (87.66%), Postives = 147/154 (95.45%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRLSSPSSLVPSSDSLENSS 60
           MGLSSLPAPSEGV+N +LVNTALSISMFKCIVRLILH++GIRLS PSSLV S+DS E+SS
Sbjct: 66  MGLSSLPAPSEGVLNVILVNTALSISMFKCIVRLILHMIGIRLSWPSSLVSSTDSFESSS 125

Query: 61  ELGDSNFGSSWSYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHL 120
           ELGDSNFGSSWSY+EMFRNRY +IRFD++QGSE REHDCSVCLTQFEPESEIN LSCGHL
Sbjct: 126 ELGDSNFGSSWSYLEMFRNRYLSIRFDKIQGSERREHDCSVCLTQFEPESEINILSCGHL 185

Query: 121 FHSECLEKWLDYWNVTCPLCRTPLMSEEEKSCFW 155
           FH+ECLEKWLDYWN+TCPLCRTPLMSEEEKSCFW
Sbjct: 186 FHTECLEKWLDYWNITCPLCRTPLMSEEEKSCFW 219

BLAST of Lag0014785 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 7.0e-38
Identity = 85/150 (56.67%), Postives = 110/150 (73.33%), Query Frame = 0

Query: 1   MGLSSLPAPSEGVINALLVNTALSISMFKCIVRLILHIVGIRL----SSPSSLVPSSDSL 60
           MGLSSLP PSEG++  +LVNTALSIS+ K IVR  L IVGI L    SSPSS+  SS++ 
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSEN- 60

Query: 61  ENSSELGDSNFGSSWSYIEMFRNRYSTIRFDEV-QGSEHREHDCSVCLTQFEPESEINHL 120
            ++SE  D       SY+E FRNR  T+RF+ + +  +  +++CSVCL++F+ +SEIN L
Sbjct: 61  SSTSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKL 120

Query: 121 SCGHLFHSECLEKWLDYWNVTCPLCRTPLM 146
            CGHLFH  CLEKW+DYWN+TCPLCRTPL+
Sbjct: 121 KCGHLFHKTCLEKWIDYWNITCPLCRTPLV 149

BLAST of Lag0014785 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-12
Identity = 34/80 (42.50%), Postives = 50/80 (62.50%), Query Frame = 0

Query: 76  MFRNRYSTIRFDEVQGS-EHREHDCSVCLTQFEPESEINHL-SCGHLFHSECLEKWLDYW 135
           + R     I+F+E+  S E    +C+VCL +FE E EI  L +C H+FH  CL++W+D+ 
Sbjct: 70  LIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHD 129

Query: 136 NVTCPLCRTPLMSEEEKSCF 154
             TCPLCRTP + +E +  F
Sbjct: 130 QKTCPLCRTPFVPDEMQEEF 149

BLAST of Lag0014785 vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.6e-10
Identity = 25/51 (49.02%), Postives = 36/51 (70.59%), Query Frame = 0

Query: 98  DCSVCLTQFEPESEINHLSCGHLFHSECLEKWLDYWNVTCPLCRTPLMSEE 149
           DC VCL++ +   E+  L C H+FH +CLE WL  +N TCPLCR+ L+S++
Sbjct: 85  DCVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDD 135

BLAST of Lag0014785 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-10
Identity = 27/74 (36.49%), Postives = 44/74 (59.46%), Query Frame = 0

Query: 72  SYIEMFRNRYSTIRFDEVQGSEHREHDCSVCLTQFEPESEINHLSCGHLFHSECLEKWLD 131
           S + +  ++ +  R    + S++   DC VCL++ +   E+  L C H+FH +CLE WL 
Sbjct: 47  SNLSVLADQLNLNRLFSYRYSDNAASDCIVCLSKLKTGEEVRKLDCRHVFHKQCLEGWLQ 106

Query: 132 YWNVTCPLCRTPLM 146
           + N  CPLCR+PL+
Sbjct: 107 HLNFNCPLCRSPLL 120

BLAST of Lag0014785 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-09
Identity = 24/48 (50.00%), Postives = 34/48 (70.83%), Query Frame = 0

Query: 94  HREHDCSVCLTQFEPESEINHLSCGHLFHSECLEKWLDYWNVTCPLCR 142
           H + +C +CL+ +E E+E+  L CGH FH  C++KWL Y N TCPLC+
Sbjct: 320 HEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWL-YINATCPLCK 366