Lag0014770 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014770
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Locationchr12: 4483231 .. 4484517 (-)
RNA-Seq ExpressionLag0014770
SyntenyLag0014770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATTAATCAGAAACCTCGCCTCAAAGATGGTGCTCTTAGACGTTTTAAGTTGGTTGTTCTTGTGGCATTATTGGTGTTATTGCTGTTTATGATCCTTATTGAAACGAATTCATTTCGGAATTTTGCTGCTTGGTCTTCTGATGATATTAGTGAGGATCCATATCTTGATTCTAAAAATGTACTGAGGCTGCCTAAACAAACTGAGTTGTCGATTCGTTTGGAAAAGCGAAATTGGTTGCCACCCCGAAACTTAGATCTATATCCAAAGCTGGCCAAGGATCATATAGCTATTGTACTTTACGTGCACAACCGACCGCAGTATCTTCGAGTGGTAGTTGATAGCCTATCGAAAGTTATAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGGTTGTCCAGAGCATCAGGTTTTGCCAGGTGAAACAGATTTTTGCTCCCTACTCTCCCCATCTGTTTCCCAACAGCTTTCCCGGAGTCTCGCCTGCAGACTGTAAGGGCAAGGATGATCCTAAAAGAATACATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGGCACCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGCTGTCGAGTCATATTTTGTTTATTGAGGAGGATCATTTTATATTGCCAAATGCATATCGAAATCTGCAAACACTTGTGACATTGAAGCCTATAAAGTGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAAGGTAATGATTTTCTTATTGCGGAGAGGATGGGAAATATCGGTTATGCTTTCAATCGGACTATTTGGAGGAAAATCCATAAAAAAGCAAAGCAGTTCTGTTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACTGTTTATCCCTCCTTTGGTAGCCCTGTTTATTCACTACGAGGGCCTTGGGCGAGTGCCATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGAGGGACATGGAAGCTTGTATTGATAATGGTGTATTAAACATTGACATTAAAGATATCGACAAAGTTGCCAACATTAATCCCAAGTGGCAAGTTGATGTTTTCAGAGATCAGCCTGGTTATGGGGCTTGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCGGTTGTGTCTAAACTTTGCTCTCATGTATCATTCCACGCCGAACGTTTCTCGTTCCTGA

mRNA sequence

ATGGCCATTAATCAGAAACCTCGCCTCAAAGATGGTGCTCTTAGACGTTTTAAGTTGGTTGTTCTTGTGGCATTATTGGTGTTATTGCTGTTTATGATCCTTATTGAAACGAATTCATTTCGGAATTTTGCTGCTTGGTCTTCTGATGATATTAGTGAGGATCCATATCTTGATTCTAAAAATGTACTGAGGCTGCCTAAACAAACTGAGTTGTCGATTCGTTTGGAAAAGCGAAATTGGTTGCCACCCCGAAACTTAGATCTATATCCAAAGCTGGCCAAGGATCATATAGCTATTGTACTTTACGTGCACAACCGACCGCAGTATCTTCGAGTGGTAGTTGATAGCCTATCGAAAGTTATAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGGTTGTCCAGAGCATCAGGTTTTGCCAGGTGAAACAGATTTTTGCTCCCTACTCTCCCCATCTGTTTCCCAACAGCTTTCCCGGAGTCTCGCCTGCAGACTGTAAGGGCAAGGATGATCCTAAAAGAATACATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGGCACCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGCTGTCGAGTCATATTTTGTTTATTGAGGAGGATCATTTTATATTGCCAAATGCATATCGAAATCTGCAAACACTTGTGACATTGAAGCCTATAAAGTGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAAGGTAATGATTTTCTTATTGCGGAGAGGATGGGAAATATCGGTTATGCTTTCAATCGGACTATTTGGAGGAAAATCCATAAAAAAGCAAAGCAGTTCTGTTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACTGTTTATCCCTCCTTTGGTAGCCCTGTTTATTCACTACGAGGGCCTTGGGCGAGTGCCATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGAGGGACATGGAAGCTTGTATTGATAATGGTGTATTAAACATTGACATTAAAGATATCGACAAAGTTGCCAACATTAATCCCAAGTGGCAAGTTGATGTTTTCAGAGATCAGCCTGGTTATGGGGCTTGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCGGTTGTGTCTAAACTTTGCTCTCATGTATCATTCCACGCCGAACGTTTCTCGTTCCTGA

Coding sequence (CDS)

ATGGCCATTAATCAGAAACCTCGCCTCAAAGATGGTGCTCTTAGACGTTTTAAGTTGGTTGTTCTTGTGGCATTATTGGTGTTATTGCTGTTTATGATCCTTATTGAAACGAATTCATTTCGGAATTTTGCTGCTTGGTCTTCTGATGATATTAGTGAGGATCCATATCTTGATTCTAAAAATGTACTGAGGCTGCCTAAACAAACTGAGTTGTCGATTCGTTTGGAAAAGCGAAATTGGTTGCCACCCCGAAACTTAGATCTATATCCAAAGCTGGCCAAGGATCATATAGCTATTGTACTTTACGTGCACAACCGACCGCAGTATCTTCGAGTGGTAGTTGATAGCCTATCGAAAGTTATAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGGTTGTCCAGAGCATCAGGTTTTGCCAGGTGAAACAGATTTTTGCTCCCTACTCTCCCCATCTGTTTCCCAACAGCTTTCCCGGAGTCTCGCCTGCAGACTGTAAGGGCAAGGATGATCCTAAAAGAATACATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGGCACCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGAAGGAGACGCGGGGGCTGTCGAGTCATATTTTGTTTATTGAGGAGGATCATTTTATATTGCCAAATGCATATCGAAATCTGCAAACACTTGTGACATTGAAGCCTATAAAGTGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAAGGTAATGATTTTCTTATTGCGGAGAGGATGGGAAATATCGGTTATGCTTTCAATCGGACTATTTGGAGGAAAATCCATAAAAAAGCAAAGCAGTTCTGTTTTTTCGATGAATACAATTGGGATATAACGATGTGGGCAACTGTTTATCCCTCCTTTGGTAGCCCTGTTTATTCACTACGAGGGCCTTGGGCGAGTGCCATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGAGGGACATGGAAGCTTGTATTGATAATGGTGTATTAAACATTGACATTAAAGATATCGACAAAGTTGCCAACATTAATCCCAAGTGGCAAGTTGATGTTTTCAGAGATCAGCCTGGTTATGGGGCTTGGTTCAAGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCGGTTGTGTCTAAACTTTGCTCTCATGTATCATTCCACGCCGAACGTTTCTCGTTCCTGA

Protein sequence

MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYLDSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKGKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDMEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFALMYHSTPNVSRS
Homology
BLAST of Lag0014770 vs. NCBI nr
Match: XP_038888419.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Benincasa hispida])

HSP 1 Score: 813.9 bits (2101), Expect = 6.5e-232
Identity = 380/429 (88.58%), Postives = 400/429 (93.24%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPR KD ALRRFKLVV VALL LLLFMIL+ETNSFRNFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRFKDAALRRFKLVVFVALLGLLLFMILMETNSFRNFAAWSSDDIDEDPYLGFD 60

Query: 61  --DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS +VLRLPKQ+ELSIRLEKRNWLPP+NLDLYPKLAKDHI IVLYVHNRPQYLRVVVD
Sbjct: 61  RFDSNDVLRLPKQSELSIRLEKRNWLPPQNLDLYPKLAKDHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SLSKVIGISETLLIVSHDGYFEEMDK+V+SIRFCQVKQIFAPYSPHLFPNSFPGVSP+DC
Sbjct: 121 SLSKVIGISETLLIVSHDGYFEEMDKLVRSIRFCQVKQIFAPYSPHLFPNSFPGVSPSDC 180

Query: 181 KGKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEED 240
           KGKDDPK IHCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKETRG+S HILFIEED
Sbjct: 181 KGKDDPKTIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETRGISGHILFIEED 240

Query: 241 HFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRT 300
           HFILPNAYRNLQ LVTL P KCP CYSVNLAPSDVKSRGEGN+FL+AERMGNIGYAFNRT
Sbjct: 241 HFILPNAYRNLQLLVTLNPKKCPHCYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRD 360
           IWRKIHK+AK+FCFFD+YNWDITMWATVYPSFG PVYSLRGPWASA+HFGKCGLHQGQ D
Sbjct: 301 IWRKIHKQAKEFCFFDDYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGQGD 360

Query: 361 MEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 420
            + CIDNG LNID++DIDKVANIN +WQVDVFR QPGYGA F+GWGGWGDERDR+LCLNF
Sbjct: 361 RDVCIDNGALNIDVRDIDKVANINSEWQVDVFRHQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 ALMYHSTPN 425
           A MYHST N
Sbjct: 421 AHMYHSTLN 429

BLAST of Lag0014770 vs. NCBI nr
Match: XP_008462069.1 (PREDICTED: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo] >KAA0059203.1 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa] >TYK19316.1 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 800.8 bits (2067), Expect = 5.7e-228
Identity = 375/429 (87.41%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPRLKD AL+RFKLVV V LL LLLFMIL ETNS  NFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  --DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS   LRLPKQ+ELSIRLEKRN LPPRNLDLYPKLAK+HI IVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SL+KVIGISETLL+VSHDGYFEEMDK+VQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKET+G+S HILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRT 300
           HFILPNAYRNLQ LVTLKP KCPECYSVNLAPSDVKSRGEG++FL+AERMGNIGYAFNRT
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRD 360
           +WRKIHK+AK+FCFFDEYNWDITMWATVYPSFG PVYSLRG WASA+HFGKCGLHQGQ D
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 MEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 420
            + CIDNGVLNID+KDIDKVANIN +W+VDVFRDQPGYGA F+GWGGWGDERDR+LCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 ALMYHSTPN 425
           A MYHS PN
Sbjct: 421 AHMYHSVPN 429

BLAST of Lag0014770 vs. NCBI nr
Match: XP_004144546.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis sativus] >KGN43409.1 hypothetical protein Csa_020290 [Cucumis sativus])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 374/434 (86.18%), Postives = 398/434 (91.71%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPR KD AL+RFKLVV V LL LLLFMIL ETN   NFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSL 120
           DS   LRLPKQ++LSIRLEKRN LPPRNLDLYPKLA +HIAIVLYVHNRPQYLRVVVDSL
Sbjct: 61  DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVDSL 120

Query: 121 SKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG 180
           +KVIGISETLLIVSHDGYFEEMDK+VQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG
Sbjct: 121 AKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG 180

Query: 181 KDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHF 240
           KDDPK +HCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKET+G+S HILFIEEDHF
Sbjct: 181 KDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEEDHF 240

Query: 241 ILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIW 300
           ILPNAYRNLQ LVTLKP KCPECYSVNLAPSDVKSRGEG++FLIAERMGNIGYAFNRT+W
Sbjct: 241 ILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNRTVW 300

Query: 301 RKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDME 360
           RKI+K+AK+FCFFDEYNWDITMWATVYPSFG PVYSLRGPWASA+HFGKCGLHQG  D +
Sbjct: 301 RKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHGDND 360

Query: 361 ACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFAL 420
            CIDNGVLNID+KDIDKVANIN +W+VD+FRDQPGYGA F+GWGGWGDERDR+LCLNFA 
Sbjct: 361 ICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLNFAR 420

Query: 421 MYHST---PNVSRS 429
           MYHS    PN+S S
Sbjct: 421 MYHSMSNGPNISHS 434

BLAST of Lag0014770 vs. NCBI nr
Match: XP_022136104.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Momordica charantia])

HSP 1 Score: 773.5 bits (1996), Expect = 9.7e-220
Identity = 360/432 (83.33%), Postives = 388/432 (89.81%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYLD-- 60
           MAINQKPRLKD AL+RFKLVVLVALLV +LF+IL+ETNSF+NF  W SD    DPYL   
Sbjct: 1   MAINQKPRLKDAALKRFKLVVLVALLVFVLFLILVETNSFQNFGPWFSD----DPYLGYD 60

Query: 61  --SKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDS 120
                VLRLPKQ+ELSIRLEKRNWLPPRNLDLYPKLAKDHI IVLYVHNRPQYLRVV++S
Sbjct: 61  HFDPKVLRLPKQSELSIRLEKRNWLPPRNLDLYPKLAKDHIPIVLYVHNRPQYLRVVIES 120

Query: 121 LSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCK 180
           LSKV+GISETLLIVSHDGYFEEMDK+VQSIRFCQ+KQIFAPYSPHLFPNSFPGVSP DCK
Sbjct: 121 LSKVVGISETLLIVSHDGYFEEMDKLVQSIRFCQMKQIFAPYSPHLFPNSFPGVSPGDCK 180

Query: 181 GKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDH 240
           GKDD K IHCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL ETRG S H+LFIEEDH
Sbjct: 181 GKDDDKIIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLNETRGRSDHVLFIEEDH 240

Query: 241 FILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTI 300
           +ILPNAYRNLQ LV LKP KCPECYSVNLAP DVKSRGE  +FL AERMGNIGYAFNRT 
Sbjct: 241 YILPNAYRNLQILVELKPKKCPECYSVNLAPCDVKSRGEDGNFLFAERMGNIGYAFNRTT 300

Query: 301 WRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDM 360
           WRKIHKKA +FCFFDEYNWDITMWATVYPSFG+PVYSLRGPWASA+HFGKCGLHQG  +M
Sbjct: 301 WRKIHKKATEFCFFDEYNWDITMWATVYPSFGNPVYSLRGPWASAVHFGKCGLHQGHGEM 360

Query: 361 EACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFA 420
           + C DNGVLNID++D+DKVANIN +W+VD F++QPGYGA F+GWGGWGDERDR+LCLNFA
Sbjct: 361 DICYDNGVLNIDVRDVDKVANINSEWEVDFFKNQPGYGAGFRGWGGWGDERDRQLCLNFA 420

Query: 421 LMYHSTPNVSRS 429
            MY ST NVSRS
Sbjct: 421 RMYSSTSNVSRS 428

BLAST of Lag0014770 vs. NCBI nr
Match: KAG7011860.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-217
Identity = 361/434 (83.18%), Postives = 390/434 (89.86%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPY---- 60
           MAINQKPRLKD ALRRFKLV+LV LL  LLFMIL+ETN F+NFA WSSD+I EDPY    
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  -LDSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS + L+LPKQTELSIRLEKRN LP RNLDLYP LAKDHI I+LYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SLSKV GISETLLIVSHDGYFEEMDK+VQ+IRFC VKQIFAPYSPHLFP+SFPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKDDPK-RIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEE 240
            GK  P    +CRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL+ETRGLS HILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LV+LKP KCPECYSVNLAPSDVKSRGEGN+FL+AERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQR 360
           ++W+KIHKKAK+FCFFDEYNWDITMWATV+PSFGSPVYSLRGPW SAIHFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DMEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLN 420
           + + CIDNG+LNID+KDIDKV NIN +W V+VFR QPGYGA F+GWGGWGDERDR+LCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FALMYHSTPNVSRS 429
           FA MY STPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Lag0014770 vs. ExPASy Swiss-Prot
Match: Q9FT88 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabidopsis thaliana OX=3702 GN=GNT2 PE=1 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 7.8e-164
Identity = 265/418 (63.40%), Postives = 330/418 (78.95%), Query Frame = 0

Query: 5   QKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYLDSK---N 64
           +K RL+D  L R  L +L A+ + ++ M++    +  N ++   D    D  L++K   +
Sbjct: 6   KKQRLRDTGLCR--LGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKLNAS 65

Query: 65  VLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVI 124
           +L + +   +S+RL +RN   PRN DL+P LAKD + IVLYVHNR QY RV V+SLSKV 
Sbjct: 66  LLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSKVK 125

Query: 125 GISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKGKDDP 184
           GISETLLIVSHDGYFEEM+++V+SI+FCQVKQIF+PYSPH++  SFPGV+  DCK K D 
Sbjct: 126 GISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKGDE 185

Query: 185 KRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPN 244
            + HC GNPDQYGNHR+PKIVSLKHHWWWMMNTVWDGL+ET+G   HILFIEEDHF+ PN
Sbjct: 186 AKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFLFPN 245

Query: 245 AYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIWRKIH 304
           AYRN+QTL  LKP KCP+C++ NLAPSDVKSRGEG + L+AERMGN+GY+FNR++W  IH
Sbjct: 246 AYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWENIH 305

Query: 305 KKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDMEACID 364
           +KA++FCFFD+YNWDITMWATV+PSFGSPVY+LRGP  SA+HFGKCGLHQG+ D   CID
Sbjct: 306 QKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGDCID 365

Query: 365 NGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFALMY 420
           NGV+NI++K+ DKV NI   W V V++ Q GY A F+GWGGWGD+RDR LCL+FA MY
Sbjct: 366 NGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFATMY 421

BLAST of Lag0014770 vs. ExPASy Swiss-Prot
Match: Q10469 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT2 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-48
Identity = 119/353 (33.71%), Postives = 184/353 (52.12%), Query Frame = 0

Query: 84  RNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVIGISETLLIVSHDGYFEEMDKVV 143
           RN+D     A   + +V+ VHNRP+YLR+++DSL K  GI   L+I SHD +  E+++++
Sbjct: 105 RNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLI 164

Query: 144 QSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKGKDDPK----RIHC--RGNPDQYGNHR 203
             + FC V Q+F P+S  L+PN FPG  P DC  +D PK    ++ C     PD +G++R
Sbjct: 165 AGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCP-RDLPKNAALKLGCINAEYPDSFGHYR 224

Query: 204 APKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPNAYRNLQTLVTLKPIKC 263
             K    KHHWWW ++ VW+ +K  R  +  ILF+EEDH++ P+ Y   + +  LK  +C
Sbjct: 225 EAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 284

Query: 264 PEC-------YSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIWRKIHKKAKQFCFF 323
           PEC       YS + +   +  + +   +   E   N+G A  R  ++K+ +    FC +
Sbjct: 285 PECDVLSLGTYSASRSFYGMADKVDVKTWKSTEH--NMGLALTRNAYQKLIECTDTFCTY 344

Query: 324 DEYNWDITM-WATV--YPSFGSPVYSLRGPWASAI-HFGKCGLHQGQ----RDMEACIDN 383
           D+YNWD T+ + TV   P F    + +  P    I H G CG+H  +        A I++
Sbjct: 345 DDYNWDWTLQYLTVSCLPKF----WKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIES 404

Query: 384 GVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 416
            + N       +   I+ K+ V V    P      +  GGWGD RD  LC ++
Sbjct: 405 LLNNNKQYMFPETLTISEKFTV-VAISPP------RKNGGWGDIRDHELCKSY 443

BLAST of Lag0014770 vs. ExPASy Swiss-Prot
Match: Q09326 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.0e-46
Identity = 114/357 (31.93%), Postives = 180/357 (50.42%), Query Frame = 0

Query: 84  RNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVIGISETLLIVSHDGYFEEMDKVV 143
           RN+D     +   + +V+ VHNRP+YLR+++DSL K  GI E L+I SHD +  E++ ++
Sbjct: 100 RNVDKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSLI 159

Query: 144 QSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCK---GKDDPKRIHC--RGNPDQYGNHRA 203
            S+ FC V Q+F P+S  L+P+ FPG  P DC     K+   ++ C     PD +G++R 
Sbjct: 160 SSVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYRE 219

Query: 204 PKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPNAYRNLQTLVTLKPIKCP 263
            K    KHHWWW ++ VW+ +K  +  +  ILF+EEDH++ P+ Y   + +  LK  +CP
Sbjct: 220 AKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECP 279

Query: 264 ECYSVNLAPSDVKSRGEGNDFLIAERMG---------NIGYAFNRTIWRKIHKKAKQFCF 323
            C  ++L          G    IA+++          N+G A  R  ++K+ +    FC 
Sbjct: 280 GCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCT 339

Query: 324 FDEYNWDITMWATVYPSFG--SPVYSLRGPWASAI-HFGKCGLHQGQRDMEACIDNGVLN 383
           +D+YNWD   W   Y +      V+ +  P A  I H G CG+H      + C      +
Sbjct: 340 YDDYNWD---WTLQYLTLACLPKVWKVLVPQAPRIFHAGDCGMHH----KKTCRP----S 399

Query: 384 IDIKDIDKVANINPKWQVDVFRDQPGYGAWF--------KGWGGWGDERDRRLCLNF 416
                I+ + N N ++   +F +    G  F        +  GGWGD RD  LC ++
Sbjct: 400 TQSAQIESLLNNNKQY---LFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSY 438

BLAST of Lag0014770 vs. ExPASy Swiss-Prot
Match: O19071 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus scrofa OX=9823 GN=MGAT2 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.3e-46
Identity = 111/352 (31.53%), Postives = 178/352 (50.57%), Query Frame = 0

Query: 84  RNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVIGISETLLIVSHDGYFEEMDKVV 143
           RN+D         + +V+ VHNR +YL++++DSL K  GI   L+I SHD +  E+++++
Sbjct: 105 RNVDKVSSWVPRELVLVVQVHNRAEYLKLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLI 164

Query: 144 QSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCK---GKDDPKRIHC--RGNPDQYGNHRA 203
             + FC V Q+F P+S  L+PN FPG  P DC     K+   ++ C     PD +G++R 
Sbjct: 165 AGVDFCPVLQVFFPFSIQLYPNEFPGTDPRDCPRDLEKNAALKMGCINAEYPDSFGHYRE 224

Query: 204 PKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPNAYRNLQTLVTLKPIKCP 263
            K    KHHWWW ++ VW+ +K  R  +  ILF+EEDH++ P+ Y   + +  LK  +CP
Sbjct: 225 AKFSQTKHHWWWKLHFVWERVKVLRDYAGLILFLEEDHYVAPDFYHVFKKMWNLKQQECP 284

Query: 264 EC-------YSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIWRKIHKKAKQFCFFD 323
           EC       Y+   +  DV  + +   +   E   N+G A  R  ++K+ +    FC +D
Sbjct: 285 ECDVLSLGTYTTVRSFRDVADKVDVKTWKSTEH--NMGLALTRDAYQKLIECTDTFCTYD 344

Query: 324 EYNWDITM-WATV--YPSFGSPVYSLRGPWASAI-HFGKCGLHQGQRDMEACIDNGVLNI 383
           +YNWD T+ + TV   P F    + +  P    I H G CG+H      + C      + 
Sbjct: 345 DYNWDWTLQYLTVSCLPKF----WKVLVPQVPRIFHAGDCGMHH----KKTCRP----ST 404

Query: 384 DIKDIDKVANINPKWQ----VDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 416
               I+ + N N ++     + +        +  +  GGWGD RD  LC ++
Sbjct: 405 QSAQIESLLNSNKQYMFPETLTISEKLTAALSPPRKNGGWGDIRDHELCKSY 442

BLAST of Lag0014770 vs. ExPASy Swiss-Prot
Match: Q921V5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.1e-46
Identity = 110/344 (31.98%), Postives = 174/344 (50.58%), Query Frame = 0

Query: 97  IAIVLYVHNRPQYLRVVVDSLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFA 156
           + +V+ VHNRP+YLR+++DSL K  GI E L+I SHD +  E++ ++  + FC V Q+F 
Sbjct: 113 LVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSLISRVDFCPVLQVFF 172

Query: 157 PYSPHLFPNSFPGVSPADCK---GKDDPKRIHC--RGNPDQYGNHRAPKIVSLKHHWWWM 216
           P+S  L+PN FPG  P DC     K+   ++ C     PD +G++R  K    KHHWWW 
Sbjct: 173 PFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWK 232

Query: 217 MNTVWDGLKETRGLSSHILFIEEDHFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVK 276
           ++ VW+ +K  +  +  ILF+EEDH++ P+ Y   + +  LK  +CP C  ++L      
Sbjct: 233 LHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPGCDVLSLGTYTTI 292

Query: 277 SRGEGNDFLIAERMG---------NIGYAFNRTIWRKIHKKAKQFCFFDEYNWDITMWAT 336
               G    IA+++          N+G A  R  ++K+ +    FC +D+YNWD   W  
Sbjct: 293 RSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFCTYDDYNWD---WTL 352

Query: 337 VYPSFG--SPVYSLRGPWASAI-HFGKCGLHQGQRDMEACIDNGVLNIDIKDIDKVANIN 396
            Y +      ++ +  P A  I H G CG+H      + C      +     I+ + N N
Sbjct: 353 QYLTLACLPKIWKVLVPQAPRIFHAGDCGMHH----KKTCRP----STQSAQIESLLNSN 412

Query: 397 PKWQVDVFRDQPGYGAWF--------KGWGGWGDERDRRLCLNF 416
            ++   +F +    G  F        +  GGWGD RD  LC ++
Sbjct: 413 KQY---LFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSY 438

BLAST of Lag0014770 vs. ExPASy TrEMBL
Match: A0A5A7UTF0 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00360 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.7e-228
Identity = 375/429 (87.41%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPRLKD AL+RFKLVV V LL LLLFMIL ETNS  NFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  --DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS   LRLPKQ+ELSIRLEKRN LPPRNLDLYPKLAK+HI IVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SL+KVIGISETLL+VSHDGYFEEMDK+VQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKET+G+S HILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRT 300
           HFILPNAYRNLQ LVTLKP KCPECYSVNLAPSDVKSRGEG++FL+AERMGNIGYAFNRT
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRD 360
           +WRKIHK+AK+FCFFDEYNWDITMWATVYPSFG PVYSLRG WASA+HFGKCGLHQGQ D
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 MEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 420
            + CIDNGVLNID+KDIDKVANIN +W+VDVFRDQPGYGA F+GWGGWGDERDR+LCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 ALMYHSTPN 425
           A MYHS PN
Sbjct: 421 AHMYHSVPN 429

BLAST of Lag0014770 vs. ExPASy TrEMBL
Match: A0A1S3CG23 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo OX=3656 GN=LOC103500510 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.7e-228
Identity = 375/429 (87.41%), Postives = 397/429 (92.54%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPRLKD AL+RFKLVV V LL LLLFMIL ETNS  NFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  --DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS   LRLPKQ+ELSIRLEKRN LPPRNLDLYPKLAK+HI IVLYVHNRPQYLRVVVD
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SL+KVIGISETLL+VSHDGYFEEMDK+VQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 KGKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEED 240
           KGKDDPK +HCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKET+G+S HILFIEED
Sbjct: 181 KGKDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEED 240

Query: 241 HFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRT 300
           HFILPNAYRNLQ LVTLKP KCPECYSVNLAPSDVKSRGEG++FL+AERMGNIGYAFNRT
Sbjct: 241 HFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNRT 300

Query: 301 IWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRD 360
           +WRKIHK+AK+FCFFDEYNWDITMWATVYPSFG PVYSLRG WASA+HFGKCGLHQGQ D
Sbjct: 301 VWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQGD 360

Query: 361 MEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNF 420
            + CIDNGVLNID+KDIDKVANIN +W+VDVFRDQPGYGA F+GWGGWGDERDR+LCLNF
Sbjct: 361 RDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLNF 420

Query: 421 ALMYHSTPN 425
           A MYHS PN
Sbjct: 421 AHMYHSVPN 429

BLAST of Lag0014770 vs. ExPASy TrEMBL
Match: A0A0A0K343 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G031700 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.7e-226
Identity = 374/434 (86.18%), Postives = 398/434 (91.71%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYL--- 60
           MAINQKPR KD AL+RFKLVV V LL LLLFMIL ETN   NFAAWSSDDI EDPYL   
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  DSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSL 120
           DS   LRLPKQ++LSIRLEKRN LPPRNLDLYPKLA +HIAIVLYVHNRPQYLRVVVDSL
Sbjct: 61  DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVDSL 120

Query: 121 SKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG 180
           +KVIGISETLLIVSHDGYFEEMDK+VQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG
Sbjct: 121 AKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKG 180

Query: 181 KDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHF 240
           KDDPK +HCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLKET+G+S HILFIEEDHF
Sbjct: 181 KDDPKAVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEEDHF 240

Query: 241 ILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIW 300
           ILPNAYRNLQ LVTLKP KCPECYSVNLAPSDVKSRGEG++FLIAERMGNIGYAFNRT+W
Sbjct: 241 ILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNRTVW 300

Query: 301 RKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDME 360
           RKI+K+AK+FCFFDEYNWDITMWATVYPSFG PVYSLRGPWASA+HFGKCGLHQG  D +
Sbjct: 301 RKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHGDND 360

Query: 361 ACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFAL 420
            CIDNGVLNID+KDIDKVANIN +W+VD+FRDQPGYGA F+GWGGWGDERDR+LCLNFA 
Sbjct: 361 ICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLNFAR 420

Query: 421 MYHST---PNVSRS 429
           MYHS    PN+S S
Sbjct: 421 MYHSMSNGPNISHS 434

BLAST of Lag0014770 vs. ExPASy TrEMBL
Match: A0A6J1C3C1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Momordica charantia OX=3673 GN=LOC111007879 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.7e-220
Identity = 360/432 (83.33%), Postives = 388/432 (89.81%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYLD-- 60
           MAINQKPRLKD AL+RFKLVVLVALLV +LF+IL+ETNSF+NF  W SD    DPYL   
Sbjct: 1   MAINQKPRLKDAALKRFKLVVLVALLVFVLFLILVETNSFQNFGPWFSD----DPYLGYD 60

Query: 61  --SKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDS 120
                VLRLPKQ+ELSIRLEKRNWLPPRNLDLYPKLAKDHI IVLYVHNRPQYLRVV++S
Sbjct: 61  HFDPKVLRLPKQSELSIRLEKRNWLPPRNLDLYPKLAKDHIPIVLYVHNRPQYLRVVIES 120

Query: 121 LSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCK 180
           LSKV+GISETLLIVSHDGYFEEMDK+VQSIRFCQ+KQIFAPYSPHLFPNSFPGVSP DCK
Sbjct: 121 LSKVVGISETLLIVSHDGYFEEMDKLVQSIRFCQMKQIFAPYSPHLFPNSFPGVSPGDCK 180

Query: 181 GKDDPKRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDH 240
           GKDD K IHCRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL ETRG S H+LFIEEDH
Sbjct: 181 GKDDDKIIHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLNETRGRSDHVLFIEEDH 240

Query: 241 FILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTI 300
           +ILPNAYRNLQ LV LKP KCPECYSVNLAP DVKSRGE  +FL AERMGNIGYAFNRT 
Sbjct: 241 YILPNAYRNLQILVELKPKKCPECYSVNLAPCDVKSRGEDGNFLFAERMGNIGYAFNRTT 300

Query: 301 WRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDM 360
           WRKIHKKA +FCFFDEYNWDITMWATVYPSFG+PVYSLRGPWASA+HFGKCGLHQG  +M
Sbjct: 301 WRKIHKKATEFCFFDEYNWDITMWATVYPSFGNPVYSLRGPWASAVHFGKCGLHQGHGEM 360

Query: 361 EACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFA 420
           + C DNGVLNID++D+DKVANIN +W+VD F++QPGYGA F+GWGGWGDERDR+LCLNFA
Sbjct: 361 DICYDNGVLNIDVRDVDKVANINSEWEVDFFKNQPGYGAGFRGWGGWGDERDRQLCLNFA 420

Query: 421 LMYHSTPNVSRS 429
            MY ST NVSRS
Sbjct: 421 RMYSSTSNVSRS 428

BLAST of Lag0014770 vs. ExPASy TrEMBL
Match: A0A6J1GLB7 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455025 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.2e-217
Identity = 361/434 (83.18%), Postives = 389/434 (89.63%), Query Frame = 0

Query: 1   MAINQKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPY---- 60
           MAINQKPRLKD ALRRFKLV+LV LL  LLFMIL+ETN F+NFA WSSD+I EDPY    
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  -LDSKNVLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVD 120
             DS + L+LPKQTELSIRLEKRN LP RNLDLYP LAKDHI IVLYVHNRPQYLRVV+D
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVIGISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180
           SLSKV GISETLLIVSHDGYFEEMDK+VQ+IRFC VKQIFAPYSPHLFP+SFPGVSP DC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 KGKDDPK-RIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEE 240
            GK  P    +CRGNPDQYGNHR PKIVSLKHHWWWMMNTVWDGL+ETRGLS HILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LV+LKP KCPECYSVNLAPSDVKSRGEGN+FL+AERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 TIWRKIHKKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQR 360
           ++W+KIHKKAK+FCFFDEYNWDITMWATV+PSFGS VYSLRGPW SAIHFGKCGLHQGQ 
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 DMEACIDNGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLN 420
           + + CIDNG+LNID+KDIDKV NIN +W V+VFR QPGYGA F+GWGGWGDERDR+LCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FALMYHSTPNVSRS 429
           FA MY STPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Lag0014770 vs. TAIR 10
Match: AT2G05320.1 (beta-1,2-N-acetylglucosaminyltransferase II )

HSP 1 Score: 578.2 bits (1489), Expect = 5.6e-165
Identity = 265/418 (63.40%), Postives = 330/418 (78.95%), Query Frame = 0

Query: 5   QKPRLKDGALRRFKLVVLVALLVLLLFMILIETNSFRNFAAWSSDDISEDPYLDSK---N 64
           +K RL+D  L R  L +L A+ + ++ M++    +  N ++   D    D  L++K   +
Sbjct: 6   KKQRLRDTGLCR--LGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKLNAS 65

Query: 65  VLRLPKQTELSIRLEKRNWLPPRNLDLYPKLAKDHIAIVLYVHNRPQYLRVVVDSLSKVI 124
           +L + +   +S+RL +RN   PRN DL+P LAKD + IVLYVHNR QY RV V+SLSKV 
Sbjct: 66  LLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSKVK 125

Query: 125 GISETLLIVSHDGYFEEMDKVVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADCKGKDDP 184
           GISETLLIVSHDGYFEEM+++V+SI+FCQVKQIF+PYSPH++  SFPGV+  DCK K D 
Sbjct: 126 GISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKGDE 185

Query: 185 KRIHCRGNPDQYGNHRAPKIVSLKHHWWWMMNTVWDGLKETRGLSSHILFIEEDHFILPN 244
            + HC GNPDQYGNHR+PKIVSLKHHWWWMMNTVWDGL+ET+G   HILFIEEDHF+ PN
Sbjct: 186 AKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFLFPN 245

Query: 245 AYRNLQTLVTLKPIKCPECYSVNLAPSDVKSRGEGNDFLIAERMGNIGYAFNRTIWRKIH 304
           AYRN+QTL  LKP KCP+C++ NLAPSDVKSRGEG + L+AERMGN+GY+FNR++W  IH
Sbjct: 246 AYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWENIH 305

Query: 305 KKAKQFCFFDEYNWDITMWATVYPSFGSPVYSLRGPWASAIHFGKCGLHQGQRDMEACID 364
           +KA++FCFFD+YNWDITMWATV+PSFGSPVY+LRGP  SA+HFGKCGLHQG+ D   CID
Sbjct: 306 QKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGDCID 365

Query: 365 NGVLNIDIKDIDKVANINPKWQVDVFRDQPGYGAWFKGWGGWGDERDRRLCLNFALMY 420
           NGV+NI++K+ DKV NI   W V V++ Q GY A F+GWGGWGD+RDR LCL+FA MY
Sbjct: 366 NGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFATMY 421

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888419.16.5e-23288.58alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Benincas... [more]
XP_008462069.15.7e-22887.41PREDICTED: alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferas... [more]
XP_004144546.11.2e-22586.18alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucumis ... [more]
XP_022136104.19.7e-22083.33alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Momordic... [more]
KAG7011860.11.5e-21783.18Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ... [more]
Match NameE-valueIdentityDescription
Q9FT887.8e-16463.40Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabid... [more]
Q104691.1e-4833.71Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo s... [more]
Q093261.0e-4631.93Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus... [more]
O190712.3e-4631.53Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus sc... [more]
Q921V55.1e-4631.98Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus mu... [more]
Match NameE-valueIdentityDescription
A0A5A7UTF02.7e-22887.41Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A1S3CG232.7e-22887.41Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A0A0K3435.7e-22686.18Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A6J1C3C14.7e-22083.33Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Momord... [more]
A0A6J1GLB72.2e-21783.18Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb... [more]
Match NameE-valueIdentityDescription
AT2G05320.15.6e-16563.40beta-1,2-N-acetylglucosaminyltransferase II [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007754N-acetylglucosaminyltransferase IIPFAMPF05060MGAT2coord: 84..412
e-value: 5.2E-100
score: 334.9
IPR007754N-acetylglucosaminyltransferase IIPANTHERPTHR12871BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE IIcoord: 7..419
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 96..355
e-value: 4.4E-28
score: 100.3
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 95..415
NoneNo IPR availablePANTHERPTHR12871:SF0ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASEcoord: 7..419

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014770.1Lag0014770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009312 oligosaccharide biosynthetic process
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0005795 Golgi stack
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008455 alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
molecular_function GO:0046872 metal ion binding