Lag0014713 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0014713
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA helicase
Location	chr12: 3861073 .. 3863886 (+)
RNA-Seq Expression	Lag0014713
Synteny	Lag0014713

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAAGATAAGGAGTTCTTCCCCATCTTACTTGGTGAGTTACTTCCCCTATATACATTTTCTCCCGTATGCATTTATAATTAGAAGCTTATGAAAAAAAAATAGAGAAAAAATAAGACCCAAGTCAAATTCCTTTAAGAAATGAAGTGGTGGTAGTGGTCTGCACTCATCAACAATCGGTGGTATCTTGGAGAATTATAATTAGTCTTTCAAATAATAATGATAAGTATCAAATTGTCAGTTTCTGTTTTGGTGTTGGTGTTCTTGCCTGAGGGGTATAGTGAGAGTTTGGGGAGGGGTAGTATTTGATGTAGCTGGTAGTCAAGCTTCTTTTCTGGTCAAAGCTTTTGTAATTATGATTGAGGGAAGTAAAGAAACTGAGACTGATTTTTTAGCTAAAGAAGAAAATCCTTTTTTTGTTTGTTTGTTTGTTTTAGTTGCTTAGTTATTTGTGTCTGTATGAGGTTTTTTTTTTAAAATGTGTCTGTATGAGGTTTATTCCTCTAGTAGGCGCTTTATTGTTTTTTTTTTTGTTTGTTTTGCTCTCTTTTCTCTCCCTTTTGGAGATTGTATCCTTGAATTTTTTTCTTCCTTTCTAATCTATCAATTAAAGTTTGTTTCTCGTTAAAAACCATGTTTGTTGGTTGACTATATTCCTCCCCAATTTGTAAAATCCTGTTTATCTTTTTTCTGTGAAATATTTGAAGTAAAATCCTGCATTTTTTGTAGGAGCCAATACACGGATACTTTCATGCTGTTTACTCTGATCACTGCTCTTGCAGTTTCAGGTACTCTGTGATTCCAAAGCCTAAGAAATGCGTGGATGATATCGATATGTTCATCAAGGAAAACCACTTTGAAGAATGTGGGATTGTTTACTGCCTTTCAAGAATGGACTGTGAAAAGGTTGCTGAAAGTTTACAGGTTGGTTTTCTCAGTCTTCTTGTATACCTGGAATATCTACAACCGGATGGTTGGTTGGTAGCAATTACAATGTGCAGAAGGGGACTTTTTAGGAAGGCAATCGCTGTTTGGAACTTTGTTAAGGATAAAAATCCAAAAGTCAACACGAATACTTTGATTTAAAAAAAAACCAAGAGTCGAATATCTAGAACCAAAGCTCCTGTCTCAGCAATTCCAAACTTTATTTTTCCTAATGGAAACAAAAATTTTCATTGATAAAATTAAAATTTAGAAATTACAAAGATAAATACAAGCATTCACAAAAGAGAAACATCCTACAATGAAGGCGAACTAATATAGAATAGTTACAAAATGAATTTGATTTAAGAAGCCCAAGGGAAAGTGTTAATACACCGAATCTCAAACATGTTCCTTCAAACTCTCTTCCTGAAAACATTTTCCTAGTTCTTTTCAGCCAAATAAACCATAAAACTTCAATGAAACCTTCTCTAGCATCTCATGAATACTTGACTTCTACACAGGCTAAATTGAAACCTTGTCAAAGACACCTGTACACTCTGGTAGTAAAAGGACATAGCCACAAAAATGATCTAGATTCTCAGCTTCGCTTCTACAAAGAGCATTGTTATGTGCTGTTGAAGTTGACATTGGTGATTAAATTGTATGTCCTAATTCAATAGATTAACTTTCAGGAATGTGGACATAAAGCATCCTTTTATCATGGTAGCATGGATCCTGCCCAACGCTCCTTCATTCAGAAGCAGTGGCTTTTGGAATGGGTATAGCTGATTATTGCTTATCAATTGATTGATATATTAAGAAATATGCACTTACTTAGATATTTCAGGTATCAACAAGCCAGATGTTCGTTTTGTCATTCACCATTCTCTTCCAAAGTCAATTGAAGGCTACCATCAGGTAATTAATTGAATCTTGCTTTATTGGGCTGGTATTGTCTACCATCAGGTAATATTGCTTTATTGGGCTGGTATTAACCAAAGCTCCTAGCTCCCCAATTCCAAACTTTTGATTTGTCTGTTAGCTTTCCAAGTTAGGTGTTTATTGTAATTGCCTTTTATTGACAAAATTAGGTAAAAAGCATAGCATAAAAAGTTGAGTCTGCATGGTGCTGGAAAACATCTCTTGAAAAGTGAAGCTTCCCGCATATTGCATCATCTTGTTATTGAGGATGTTCTTGTGGAAGAAGTGAGGAAAAATGATATTTATGGATCTGTCTCATCCTTACTGAAGGTACTAAACTAATTGTTTCATTGTATATACTAAATGGAATAAAAAGAATAGTTTCTAAGATTATTATCTTCTTCCTCCTTTTTATTTTTTCTTATTTTTTGACAATTTTGTATAATATTTTCTTGCTGCTTTTGCTTTTCTAGTTGTCTATATTAAAATGTTCACGATGTTTCAATTTGTCATCAGGTGAACGAATCCAAGGCTCACAGTCTCTTTAATGGAGGGCAGCGAGTTACATTAAGGTTAAATATTTTGTTCCTCCTAATAGTTATTTGTTGGCGTGCAATTTCTATGACAACATACAAGTCATAAGTCAATATTCATATCTCTGTGGTATAAGTATTGAAATTGACGTGTCATCAACCTATCATACCTTTTTTTTTTTCTTTTTTCTTTTTTTTAAAGAAACATTTCCTTAAAAGGATTCCTTTTAAGGAGAGTATGACAAGGTGTCCTGATCCACTTTACAAACCACAAGAGATCAGGAAAAGTTTCTCAATTGGTTTTTCACTTGTAAAAGAAAAAAAGTTAGTTCTTAGTGAAAGTACTTATGACCTACGTTTCACTGGATTTGGGAAAACAATTGAAGTTTGTACATTAGAAAAGGCAAAAGAACAATATACAGATGCTGACTAG

mRNA sequence

ATGGGAGAAGATAAGGAGTTCTTCCCCATCTTACTTGTGAGAGTTTGGGGAGGGGTAGTATTTGATGTAGCTGGTAGTCAAGCTTCTTTTCTGGTCAAAGCTTTTGAGCCAATACACGGATACTTTCATGCTGTTTACTCTGATCACTGCTCTTGCAGTTTCAGGTACTCTGTGATTCCAAAGCCTAAGAAATGCGTGGATGATATCGATATGTTCATCAAGGAAAACCACTTTGAAGAATGTGGGATTGTTTACTGCCTTTCAAGAATGGACTGTGAAAAGGTTGCTGAAAGTTTACAGGAATGTGGACATAAAGCATCCTTTTATCATGGTAGCATGGATCCTGCCCAACGCTCCTTCATTCAGAAGCAGTGGCTTTTGGAATGGGTATCAACAAGCCAGATGTTCGTTTTGTCATTCACCATTCTCTTCCAAAGTCAATTGAAGGCTACCATCAGTGAAGCTTCCCGCATATTGCATCATCTTGTTATTGAGGATGTTCTTGTGGAAGAAGTGAGGAAAAATGATATTTATGGATCTGTCTCATCCTTACTGAAGGTGAACGAATCCAAGGCTCACAGTCTCTTTAATGGAGGGCAGCGAGTTACATTAAGGATTCCTTTTAAGGAGAGTATGACAAGGTGTCCTGATCCACTTTACAAACCACAAGAGATCAGGAAAAGTTTCTCAATTGGTTTTTCACTTGTAAAAGAAAAAAAGTTAGTTCTTAGTGAAAGTACTTATGACCTACGTTTCACTGGATTTGGGAAAACAATTGAAGTTTGTACATTAGAAAAGGCAAAAGAACAATATACAGATGCTGACTAG

Coding sequence (CDS)

ATGGGAGAAGATAAGGAGTTCTTCCCCATCTTACTTGTGAGAGTTTGGGGAGGGGTAGTATTTGATGTAGCTGGTAGTCAAGCTTCTTTTCTGGTCAAAGCTTTTGAGCCAATACACGGATACTTTCATGCTGTTTACTCTGATCACTGCTCTTGCAGTTTCAGGTACTCTGTGATTCCAAAGCCTAAGAAATGCGTGGATGATATCGATATGTTCATCAAGGAAAACCACTTTGAAGAATGTGGGATTGTTTACTGCCTTTCAAGAATGGACTGTGAAAAGGTTGCTGAAAGTTTACAGGAATGTGGACATAAAGCATCCTTTTATCATGGTAGCATGGATCCTGCCCAACGCTCCTTCATTCAGAAGCAGTGGCTTTTGGAATGGGTATCAACAAGCCAGATGTTCGTTTTGTCATTCACCATTCTCTTCCAAAGTCAATTGAAGGCTACCATCAGTGAAGCTTCCCGCATATTGCATCATCTTGTTATTGAGGATGTTCTTGTGGAAGAAGTGAGGAAAAATGATATTTATGGATCTGTCTCATCCTTACTGAAGGTGAACGAATCCAAGGCTCACAGTCTCTTTAATGGAGGGCAGCGAGTTACATTAAGGATTCCTTTTAAGGAGAGTATGACAAGGTGTCCTGATCCACTTTACAAACCACAAGAGATCAGGAAAAGTTTCTCAATTGGTTTTTCACTTGTAAAAGAAAAAAAGTTAGTTCTTAGTGAAAGTACTTATGACCTACGTTTCACTGGATTTGGGAAAACAATTGAAGTTTGTACATTAGAAAAGGCAAAAGAACAATATACAGATGCTGACTAG

Protein sequence

MGEDKEFFPILLVRVWGGVVFDVAGSQASFLVKAFEPIHGYFHAVYSDHCSCSFRYSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDPAQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHHLVIEDVLVEEVRKNDIYGSVSSLLKVNESKAHSLFNGGQRVTLRIPFKESMTRCPDPLYKPQEIRKSFSIGFSLVKEKKLVLSESTYDLRFTGFGKTIEVCTLEKAKEQYTDAD

Homology

[Close]

BLAST of Lag0014713 vs. NCBI nr
Match: GFY88536.1 (DNA helicase [Actinidia rufa])

HSP 1 Score: 173.3 bits (438), Expect = 2.8e-39
Identity = 109/278 (39.21%), Postives = 137/278 (49.28%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCV+DID FIK+NHF+ECGI+YCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 511 YSVIPKTKKCVEDIDGFIKKNHFDECGIIYCLSRMDCEKVAEKLQECGHKAAFYHGSMDP 570

Query: 116 AQRSFIQKQWLLEWVS-------------------------------------------- 175
           AQR+FIQKQW  + ++                                            
Sbjct: 571 AQRAFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQ 630

Query: 176 ----------------------------------TSQMFV--LSFTILFQSQLK------ 215
                                              +Q+FV   ++TIL    +K      
Sbjct: 631 HSSCVLYYSYSDYIRVQHMITQGAVEQSPMPSGYKNQLFVNLWNWTILQVEVVKLTGQQF 690

[Close]

BLAST of Lag0014713 vs. NCBI nr
Match: XP_008456059.1 (PREDICTED: ATP-dependent DNA helicase Q-like 4A isoform X2 [Cucumis melo])

HSP 1 Score: 168.7 bits (426), Expect = 7.0e-38
Identity = 117/308 (37.99%), Postives = 132/308 (42.86%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 654 YSVIPKTKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 713

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 714 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQIRVKHMISQG 773

Query: 176 ---------------------------------------------LLEWV---------- 208
                                                        LL+ V          
Sbjct: 774 ATEQSPLVSGYNRTNLGSSGRILETNTENLLRMVSYCENDVDCRRLLQLVHFGEKFDPGN 833

[Close]

BLAST of Lag0014713 vs. NCBI nr
Match: KAG6576984.1 (ATP-dependent DNA helicase Q-like 4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 168.3 bits (425), Expect = 9.2e-38
Identity = 117/304 (38.49%), Postives = 130/304 (42.76%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSV+PK KKCVDDID FIK+NHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 600 YSVMPKTKKCVDDIDKFIKDNHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 659

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 660 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 719

Query: 176 ------------------------------------------------------------ 208
                                                                       
Sbjct: 720 RSSCVLYYSYSDYIRVKHMISQGATEQSPLVSGYNRTNLGSTGRILETNTENLLRMVSYC 779

[Close]

BLAST of Lag0014713 vs. NCBI nr
Match: XP_022149253.1 (LOW QUALITY PROTEIN: ATP-dependent DNA helicase Q-like 4A [Momordica charantia])

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-35
Identity = 119/331 (35.95%), Postives = 132/331 (39.88%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMD 
Sbjct: 655 YSVIPKAKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAEKLQECGHKAAFYHGSMDS 714

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
            QR+FIQKQW                                                  
Sbjct: 715 TQRAFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 774

Query: 176 ----------------------------------------------------LLEWVSTS 208
                                                               LL  VS  
Sbjct: 775 RSSCVLYYSYSDYIRVKHMISQGAAEQSPQVSGYNRTNVGSSGRILETNTENLLRMVSYC 834

[Close]

BLAST of Lag0014713 vs. NCBI nr
Match: XP_011651280.1 (ATP-dependent DNA helicase Q-like 4A isoform X1 [Cucumis sativus])

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-35
Identity = 119/331 (35.95%), Postives = 131/331 (39.58%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 654 YSVIPKTKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 713

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 714 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 773

Query: 176 ------------------------------------------------------------ 208
                                                                       
Sbjct: 774 RSSCVLYYSYSDYIRVKHMISQGATEQSPLVSGYNRTNLGSSGRILETNTENLLRMVSYC 833

[Close]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot
Match: Q8L840 (ATP-dependent DNA helicase Q-like 4A OS=Arabidopsis thaliana OX=3702 GN=RECQL4A PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 60/106 (56.60%), Postives = 78/106 (73.58%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSV+PK KKC++DID FIKENHF+ECGI+YCLSRMDCEKV+E LQE GHKA+FYHGSM+P
Sbjct: 650 YSVVPKTKKCLEDIDKFIKENHFDECGIIYCLSRMDCEKVSERLQEFGHKAAFYHGSMEP 709

Query: 116 AQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            QR+FIQ QW     S  ++ ++  T+ F   +     +   ++HH
Sbjct: 710 EQRAFIQTQW-----SKDEINIICATVAFGMGINK--PDVRFVIHH 748

HSP 2 Score: 72.8 bits (177), Expect = 6.9e-12
Identity = 34/54 (62.96%), Postives = 45/54 (83.33%), Query Frame = 0


Query: 154 EASRILHHLVIEDVLVEEVRKNDIYGSVSSLLKVNESKAHSLFNGGQRVTLRIP 208
           E SRILH+LV ED+LVE+VRK+D+YGSVSSLL+VN +KA  LF+G Q + ++ P
Sbjct: 925 EVSRILHYLVTEDILVEDVRKSDMYGSVSSLLQVNNAKATILFSGSQTIVMKFP 978

[Close]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot
Match: Q9FT70 (ATP-dependent DNA helicase Q-like 4B OS=Arabidopsis thaliana OX=3702 GN=RECQL4B PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.2e-28
Identity = 56/106 (52.83%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSV+PK  KC++DID FI+ENHF+ECGI+YCLSRMDCEKV E+L+  GHKA+FYHGSMDP
Sbjct: 666 YSVVPKTNKCLEDIDKFIRENHFDECGIIYCLSRMDCEKVTEALRVFGHKAAFYHGSMDP 725

Query: 116 AQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            +R+F+QKQW     S  ++ ++  T+ F   +     +   ++HH
Sbjct: 726 GKRAFVQKQW-----SKDEINIICATVAFGMGINK--PDVRFVIHH 764

HSP 2 Score: 79.0 bits (193), Expect = 9.6e-14
Identity = 39/57 (68.42%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 151 TISEASRILHHLVIEDVLVEEVRKNDIYGSVSSLLKVNESKAHSLFNGGQRVTLRIP 208
           T SEASRILH+LV ED+L E V+K+++YGSVSSLLKVN SKA SL +GGQ +T+R P
Sbjct: 938 TKSEASRILHYLVTEDILAEGVKKSELYGSVSSLLKVNRSKAASLLSGGQSITMRFP 994

[Close]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot
Match: Q9TXJ8 (Putative ATP-dependent DNA helicase Q1 OS=Caenorhabditis elegans OX=6239 GN=K02F3.12 PE=3 SV=3)

HSP 1 Score: 69.7 bits (169), Expect = 5.8e-11
Identity = 32/77 (41.56%), Postives = 47/77 (61.04%), Query Frame = 0


Query: 53  SFRYSVIPKP---KKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFY 112
           + +Y V+ KP    +C ++I   IK +   + GI+YCLSR DCEKVA++L+  G KA  Y
Sbjct: 303 NLKYKVVQKPGSEDECTEEIAKTIKRDFAGQTGIIYCLSRNDCEKVAKALKSHGIKAKHY 362

Query: 113 HGSMDPAQRSFIQKQWL 127
           H  M+P  RS   + W+
Sbjct: 363 HAYMEPVDRSGAHQGWI 379

[Close]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot
Match: A8WK63 (Putative ATP-dependent DNA helicase Q1 OS=Caenorhabditis briggsae OX=6238 GN=CBG24191 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.9e-10
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 53  SFRYSVIPKP---KKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFY 112
           +  Y V+ KP    +CV+ I   IK     + GI+YCLSR DCEK+A+SL+  G +A  Y
Sbjct: 280 NLNYKVLTKPGSEDECVEKIVRTIKRKFSGKTGIIYCLSRNDCEKLAKSLKANGIRAKHY 339

Query: 113 HGSMDPAQRSFIQKQWL 127
           H  M+P  RS   ++W+
Sbjct: 340 HAYMEPVDRSAAHQKWV 356

[Close]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot
Match: Q9DEY9 (Bloom syndrome protein homolog OS=Xenopus laevis OX=8355 GN=blm PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.4e-10
Identity = 28/75 (37.33%), Postives = 49/75 (65.33%), Query Frame = 0


Query: 53  SFRYSVIP-KPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHG 112
           + +Y V+P KPK+   D   +IK++H  + GI+YCLSR +C+ +A++LQ+ G  A  YH 
Sbjct: 813 NLKYEVLPKKPKRVALDCVEWIKKHHPNDSGIIYCLSRHECDTMADTLQKEGLAALAYHA 872

Query: 113 SMDPAQRSFIQKQWL 127
            +  + R ++Q +W+
Sbjct: 873 GLADSNRDYVQHKWI 887

[Close]

BLAST of Lag0014713 vs. ExPASy TrEMBL
Match: A0A7J0EQR9 (DNA helicase OS=Actinidia rufa OX=165716 GN=Acr_06g0004760 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 1.4e-39
Identity = 109/278 (39.21%), Postives = 137/278 (49.28%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCV+DID FIK+NHF+ECGI+YCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 511 YSVIPKTKKCVEDIDGFIKKNHFDECGIIYCLSRMDCEKVAEKLQECGHKAAFYHGSMDP 570

Query: 116 AQRSFIQKQWLLEWVS-------------------------------------------- 175
           AQR+FIQKQW  + ++                                            
Sbjct: 571 AQRAFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGQ 630

Query: 176 ----------------------------------TSQMFV--LSFTILFQSQLK------ 215
                                              +Q+FV   ++TIL    +K      
Sbjct: 631 HSSCVLYYSYSDYIRVQHMITQGAVEQSPMPSGYKNQLFVNLWNWTILQVEVVKLTGQQF 690

[Close]

BLAST of Lag0014713 vs. ExPASy TrEMBL
Match: A0A1S3C1X6 (DNA helicase OS=Cucumis melo OX=3656 GN=LOC103496104 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 3.4e-38
Identity = 117/308 (37.99%), Postives = 132/308 (42.86%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 654 YSVIPKTKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 713

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 714 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQIRVKHMISQG 773

Query: 176 ---------------------------------------------LLEWV---------- 208
                                                        LL+ V          
Sbjct: 774 ATEQSPLVSGYNRTNLGSSGRILETNTENLLRMVSYCENDVDCRRLLQLVHFGEKFDPGN 833

[Close]

BLAST of Lag0014713 vs. ExPASy TrEMBL
Match: A0A0A0L762 (DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_3G221760 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 119/331 (35.95%), Postives = 131/331 (39.58%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 634 YSVIPKTKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 693

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 694 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 753

Query: 176 ------------------------------------------------------------ 208
                                                                       
Sbjct: 754 RSSCVLYYSYSDYIRVKHMISQGATEQSPLVSGYNRTNLGSSGRILETNTENLLRMVSYC 813

[Close]

BLAST of Lag0014713 vs. ExPASy TrEMBL
Match: A0A6J1D6B6 (DNA helicase OS=Momordica charantia OX=3673 GN=LOC111017717 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 119/331 (35.95%), Postives = 132/331 (39.88%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMD 
Sbjct: 655 YSVIPKAKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAEKLQECGHKAAFYHGSMDS 714

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
            QR+FIQKQW                                                  
Sbjct: 715 TQRAFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 774

Query: 176 ----------------------------------------------------LLEWVSTS 208
                                                               LL  VS  
Sbjct: 775 RSSCVLYYSYSDYIRVKHMISQGAAEQSPQVSGYNRTNVGSSGRILETNTENLLRMVSYC 834

[Close]

BLAST of Lag0014713 vs. ExPASy TrEMBL
Match: A0A1S3C2C0 (DNA helicase OS=Cucumis melo OX=3656 GN=LOC103496104 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.6e-35
Identity = 117/331 (35.35%), Postives = 132/331 (39.88%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSVIPK KKCVDDID FIKENHF+ECGIVYCLSRMDCEKVAE LQECGHKA+FYHGSMDP
Sbjct: 587 YSVIPKTKKCVDDIDKFIKENHFDECGIVYCLSRMDCEKVAERLQECGHKAAFYHGSMDP 646

Query: 116 AQRSFIQKQW-------------------------------------------------- 175
           AQRSFIQKQW                                                  
Sbjct: 647 AQRSFIQKQWSKDEINIICATVAFGMGINKPDVRFVIHHSLPKSIEGYHQECGRAGRDGL 706

Query: 176 ------------------------------------------------------------ 208
                                                                       
Sbjct: 707 RSSCVLYYSYSDYIRVKHMISQGATEQSPLVSGYNRTNLGSSGRILETNTENLLRMVSYC 766

[Close]

BLAST of Lag0014713 vs. TAIR 10
Match: AT1G10930.1 (DNA helicase (RECQl4A) )

HSP 1 Score: 131.0 bits (328), Expect = 1.5e-30
Identity = 60/106 (56.60%), Postives = 78/106 (73.58%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSV+PK KKC++DID FIKENHF+ECGI+YCLSRMDCEKV+E LQE GHKA+FYHGSM+P
Sbjct: 650 YSVVPKTKKCLEDIDKFIKENHFDECGIIYCLSRMDCEKVSERLQEFGHKAAFYHGSMEP 709

Query: 116 AQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            QR+FIQ QW     S  ++ ++  T+ F   +     +   ++HH
Sbjct: 710 EQRAFIQTQW-----SKDEINIICATVAFGMGINK--PDVRFVIHH 748

HSP 2 Score: 72.8 bits (177), Expect = 4.9e-13
Identity = 34/54 (62.96%), Postives = 45/54 (83.33%), Query Frame = 0


Query: 154 EASRILHHLVIEDVLVEEVRKNDIYGSVSSLLKVNESKAHSLFNGGQRVTLRIP 208
           E SRILH+LV ED+LVE+VRK+D+YGSVSSLL+VN +KA  LF+G Q + ++ P
Sbjct: 925 EVSRILHYLVTEDILVEDVRKSDMYGSVSSLLQVNNAKATILFSGSQTIVMKFP 978

[Close]

BLAST of Lag0014713 vs. TAIR 10
Match: AT1G60930.1 (RECQ helicase L4B )

HSP 1 Score: 126.3 bits (316), Expect = 3.7e-29
Identity = 56/106 (52.83%), Postives = 77/106 (72.64%), Query Frame = 0


Query: 56  YSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQECGHKASFYHGSMDP 115
           YSV+PK  KC++DID FI+ENHF+ECGI+YCLSRMDCEKV E+L+  GHKA+FYHGSMDP
Sbjct: 666 YSVVPKTNKCLEDIDKFIRENHFDECGIIYCLSRMDCEKVTEALRVFGHKAAFYHGSMDP 725

Query: 116 AQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            +R+F+QKQW     S  ++ ++  T+ F   +     +   ++HH
Sbjct: 726 GKRAFVQKQW-----SKDEINIICATVAFGMGINK--PDVRFVIHH 764

HSP 2 Score: 79.0 bits (193), Expect = 6.8e-15
Identity = 39/57 (68.42%), Postives = 48/57 (84.21%), Query Frame = 0


Query: 151 TISEASRILHHLVIEDVLVEEVRKNDIYGSVSSLLKVNESKAHSLFNGGQRVTLRIP 208
           T SEASRILH+LV ED+L E V+K+++YGSVSSLLKVN SKA SL +GGQ +T+R P
Sbjct: 938 TKSEASRILHYLVTEDILAEGVKKSELYGSVSSLLKVNRSKAASLLSGGQSITMRFP 994

[Close]

BLAST of Lag0014713 vs. TAIR 10
Match: AT1G31360.1 (RECQ helicase L2 )

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-09
Identity = 38/110 (34.55%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 56  YSVIPKP---KKCVDDIDMFIKENHF-EECGIVYCLSRMDCEKVAESLQECGHKASFYHG 115
           YSV  K    K  VD+I  FI+E++   E GIVYC SR +CE++A  L+E G  A +YH 
Sbjct: 286 YSVREKSAVGKLVVDEIAEFIRESYSNNESGIVYCFSRKECEQIAGDLRERGISADYYHA 345

Query: 116 SMDPAQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            MD   R  +  +W     S +++ V+  T+ F   +     +   ++HH
Sbjct: 346 DMDANMREKVHMRW-----SKNKLQVIVGTVAFGMGINK--PDVRFVIHH 388

[Close]

BLAST of Lag0014713 vs. TAIR 10
Match: AT1G31360.2 (RECQ helicase L2 )

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-09
Identity = 38/110 (34.55%), Postives = 58/110 (52.73%), Query Frame = 0


Query: 56  YSVIPKP---KKCVDDIDMFIKENHF-EECGIVYCLSRMDCEKVAESLQECGHKASFYHG 115
           YSV  K    K  VD+I  FI+E++   E GIVYC SR +CE++A  L+E G  A +YH 
Sbjct: 161 YSVREKSAVGKLVVDEIAEFIRESYSNNESGIVYCFSRKECEQIAGDLRERGISADYYHA 220

Query: 116 SMDPAQRSFIQKQWLLEWVSTSQMFVLSFTILFQSQLKATISEASRILHH 162
            MD   R  +  +W     S +++ V+  T+ F   +     +   ++HH
Sbjct: 221 DMDANMREKVHMRW-----SKNKLQVIVGTVAFGMGINK--PDVRFVIHH 263

[Close]

BLAST of Lag0014713 vs. TAIR 10
Match: AT3G05740.1 (RECQ helicase l1 )

HSP 1 Score: 56.2 bits (134), Expect = 4.7e-08
Identity = 29/93 (31.18%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 53  SFRYSVIPKPKKCVDDIDMFIKENHFEECGIVYCLSRMDCEKVAESLQE-CGHKASFYHG 112
           + +Y VI K K+ +  +   +++   ++ GIVYCLS+ +C  VA+ L E C  K  +YH 
Sbjct: 401 NLKYEVIVKTKEPLKQLQELLRDRFKDQSGIVYCLSKSECVDVAKFLNEKCKVKTVYYHA 460

Query: 113 SMDPAQRSFIQKQWLLEWVSTSQMFVLSFTILF 145
            +   QR  +Q++W      T ++ ++  TI F
Sbjct: 461 GVPAKQRVDVQRKW-----QTGEVRIVCATIAF 488

The following BLAST results are available for this feature:

BLAST of Lag0014713 vs. NCBI nr


Match Name	E-value	Identity	Description
GFY88536.1	2.8e-39	39.21	DNA helicase [Actinidia rufa]	[more]
XP_008456059.1	7.0e-38	37.99	PREDICTED: ATP-dependent DNA helicase Q-like 4A isoform X2 [Cucumis melo]	[more]
KAG6576984.1	9.2e-38	38.49	ATP-dependent DNA helicase Q-like 4A, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_022149253.1	1.9e-35	35.95	LOW QUALITY PROTEIN: ATP-dependent DNA helicase Q-like 4A [Momordica charantia]	[more]
XP_011651280.1	1.9e-35	35.95	ATP-dependent DNA helicase Q-like 4A isoform X1 [Cucumis sativus]	[more]

BLAST of Lag0014713 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L840	2.1e-29	56.60	ATP-dependent DNA helicase Q-like 4A OS=Arabidopsis thaliana OX=3702 GN=RECQL4A ...	[more]
Q9FT70	5.2e-28	52.83	ATP-dependent DNA helicase Q-like 4B OS=Arabidopsis thaliana OX=3702 GN=RECQL4B ...	[more]
Q9TXJ8	5.8e-11	41.56	Putative ATP-dependent DNA helicase Q1 OS=Caenorhabditis elegans OX=6239 GN=K02F...	[more]
A8WK63	2.9e-10	41.56	Putative ATP-dependent DNA helicase Q1 OS=Caenorhabditis briggsae OX=6238 GN=CBG...	[more]
Q9DEY9	8.4e-10	37.33	Bloom syndrome protein homolog OS=Xenopus laevis OX=8355 GN=blm PE=2 SV=1	[more]

BLAST of Lag0014713 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7J0EQR9	1.4e-39	39.21	DNA helicase OS=Actinidia rufa OX=165716 GN=Acr_06g0004760 PE=3 SV=1	[more]
A0A1S3C1X6	3.4e-38	37.99	DNA helicase OS=Cucumis melo OX=3656 GN=LOC103496104 PE=3 SV=1	[more]
A0A0A0L762	9.3e-36	35.95	DNA helicase OS=Cucumis sativus OX=3659 GN=Csa_3G221760 PE=3 SV=1	[more]
A0A6J1D6B6	9.3e-36	35.95	DNA helicase OS=Momordica charantia OX=3673 GN=LOC111017717 PE=3 SV=1	[more]
A0A1S3C2C0	1.6e-35	35.35	DNA helicase OS=Cucumis melo OX=3656 GN=LOC103496104 PE=3 SV=1	[more]

BLAST of Lag0014713 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10930.1	1.5e-30	56.60	DNA helicase (RECQl4A)	[more]
AT1G60930.1	3.7e-29	52.83	RECQ helicase L4B	[more]
AT1G31360.1	2.5e-09	34.55	RECQ helicase L2	[more]
AT1G31360.2	2.5e-09	34.55	RECQ helicase L2	[more]
AT3G05740.1	4.7e-08	31.18	RECQ helicase l1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 53..146 e-value: 1.6E-14 score: 55.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 55..123
None	No IPR available	PANTHER	PTHR13710:SF128	ATP-DEPENDENT DNA HELICASE Q-LIKE 4A	coord: 153..215
None	No IPR available	PANTHER	PTHR13710	DNA HELICASE RECQ FAMILY MEMBER	coord: 153..215 coord: 54..125
None	No IPR available	PANTHER	PTHR13710:SF128	ATP-DEPENDENT DNA HELICASE Q-LIKE 4A	coord: 54..125
IPR001763	Rhodanese-like domain	PROSITE	PS50206	RHODANESE_3	coord: 83..125 score: 9.072996

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0014713.1	Lag0014713.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity