Lag0014700 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0014700
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	kinetochore protein NUF2 homolog
Location	chr12: 3689544 .. 3693190 (-)
RNA-Seq Expression	Lag0014700
Synteny	Lag0014700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAAGTATGAATTTCCCAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGTAATTCCCTTGCTTATGAAATTTCCTTTAACAAGTATTTGAACTGTCTTTTGAAAGGAAGGAGAAAGAGAAAAGTTTATTGATGTGTTACTATGTCAGTCAGCATTCACTTCTTTCAACGTTTTGATATCTGTGAGGTTTATAGTGTGGATTTGATGTCAAACAACTTTACTTGGTCAACTTTTTATTTCTTGCTTCTAAGAAAATTTAATATTGCATAGAATGGTAGGATAATGGTTTTATCTCTCCGTTTCTTGATAATAGGAATTGCTGTGACCCATAATTCACGTTTTGTTTCCTAGAGAATTCAAATTATGGAAGTTTTTTCTTCTCTTTGGAAGAGTAAAATACCAAAGTAGGTCAAAGCCTGCGTGGTTGGTTTTGCACGGATGTGTCTTTATAGGAGTCTATTTCCTGACCTTTTGGTAATTATGAGCTTGGTTTGGTCCTCTTGGACTGAAGTCCATTTCTTTAGCTAGTGTTAGAACTCTTTTTTGTGGGCTTATTGTTGTAGGCCCTTGTACATTCTTTCATTTTTTCAATGAAAGCTCGGTTTCTTACTTAAAAAAAATATCAATGTGTTCATGAAGTTCCCATACTCCTTTTCCATTCTAGATCTTTGGAGTGTCATACTGTGAACTTGTTTATTTTTCTATGCTTAGAATCCTTTTTAAGATAGGTTATATTTCAATTTTGTTCGTTTTTTGCACAGGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTTGAGAACCCTGATCTGCATATGGACTCAGTTCGGATAATGAAGTTGCACAACAAAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAGTACGAACCTTATCTTCTTCTCTATGGTTGTCACTTTCTCTTTACGAGAAAAAAAGAACACTAGCGTAACAAACTATTAGTTTGAAGGTCTTTATGGGTTTATCAACTTAGGGTGTAGTATCTCAACCAAGGAATTTCTACTCAATTCTCAGCTTATTATTGGCATTCTAGTCAGTTGTCAATAATTGTTAGTAAACATCTCAATTAAATGAGTTTATTATTTAACTACCAGTTACACTTATGTTTGCCCCATTTATATTAGTTTAGCTCATCACATTATATTGAATGAAAGTGACAGCTTCTTGCTTATTGTTAATTTGATTATAGGCTTAATTAGATGTTTTTGTAGTTCAATGGCATTGTGCAGAAGTCAACATATTCTGATAAATTTTCTAGCTTGTCATGGTAGAGGTTTATTTTCTTTATATGATTGGATGAATGAGATGAAGTAACGCCAATTTATCACTTCTATTTATTATATTTTATTGTTGTCATGCTTTCTTATTTGTAATACACCCCACGGGCTGGGACACAGCTCCATGCTTTCGTGTTGGTAATTACATCCAAAAGCTGAATTTTCTTATTGAATTGGCCGGGACTTCCCTACAGGGTGTTTTGATAAGGATATGTATTATTTTATTGTCTTTTAAATAGGTTTCATTATTATAATCTCTAAAATGTTGCATGATATATATCTATCGAAATATGTTTTTTCATACAAGAATAGATAGATTTATATTGACTCAGTTATGACTCTTGTATTTCCATTTGAAGAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGGTATTTAGATTTCATCCCTTTTTCATTTTTATTTAATATTATTTTATTTTAAATTTGAAAAAGGGTGGACATATATATTTCTTTTTCCATTTGGCTGAACTTGTGTGTTACAGTTGAATGCAGAAATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGGTTAGATTATTAACAACTTTTTCTTTTTTCTATTTCTCTTTTTGGGATAGGGAACTGATACTTGTATATTCCTTGTTGAGAGAAGTTGCTCGAGTCAAACCATTTCTTCACAAACATTTTCTTTGAGAATAATATCATTGACATACACTATTTAACATAGCAGTTTTTTTTTTTTTTTTGGTTAGAAATGTTTGATGATAAGGTGTGATCAGATTGACACTAATCATAGCCATCTTGCTTTAACACATTTATAAAGTTTCAAACCAAGCCCGAGGTGATTGTTTTAGGCTATAAAGAGGTTTCTTGAGTTTACATTCCTTTCCTCTAGTATAATGATCCTCAAATCTAAGAGATGTATCCATATACATTTCTTCTATTGGCCATCATTAAAATGCATCTTTTACATTGAGTAGAAATTGTAGTCTATCTAGATATGTTACAAGAGAGAGAAGTACCCAAATAATATTTAACTCGGCTGTTGGAGAAAACGTTTCCTAATAGTTCATCGCTTAAGCTTGGATGAATCCTTTAGCCACAATACAAAAGGGTCCAATAGTAGAGTTGAAAAAATCATGAGCTTTATTAGGGACTTATTTGACACAAATCTAAAAGTATAGAGATTAAATTTGTAATTTAAATTTAAATTTAAAATGTTTAAGAGGTCACTCCAACATAATGTCGTAAGGTAAGAGGATAATAATTGTTAGAATCTGGAACAATCACCAAGGCTTTATTGTTTGTGTAACATTCGGGTGGTGCCAAATTCCTTGCAACGGAGTTCGCTCGTAGGTTGGAACTACACTGGAAACAATGCAACACGAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAGAAAGATTGCAAAAACAATATGAGGAATACAAGCTTCAAAAGATGGGGAGAGAAGAAAAGCGGAAAGAAGAGAAGCAGAGAGAGCATAAATGCAAAAGTATGGAGCATAGGAATTTTGAAGGAGGAGCAAAGTCAGGAAGAAGAAGTCGACTAGAAAAGGTAAGAAGTTGAAGCAATTGATGGAAGATCGACGTGCAATCTACCTCAACTAAAAACCAAGCATGAAAGGGTTGGAAAAGTATCTGAAACGACGAAGGTAAAATTCTTGAAGCAACATCATCGACCATCATCGAGGGAGGGACGATTCAGTAACCGCAAGTTTCAAATGACAAGCAGAGGAAGACAACGGTACAGTCGATGTCAAGGCGGTCGACCCTGACGATGGAAGAGCAAAAGTCCAACGAGTACATCAGAGGAACAAGAATGTTGGCGGGCAAAGAAGTGGCAAAACGAACAACTTCGACAATGGAAATTTCACAAGAAAGAGAAAGAAAAAGCTAAGAGGGAAAGGAGGAAAGAAATTTTGA

mRNA sequence

ATGTCAAAGTATGAATTTCCCAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTTGAGAACCCTGATCTGCATATGGACTCAGTTCGGATAATGAAGTTGCACAACAAAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGTTGAATGCAGAAATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGGTTGGAACTACACTGGAAACAATGCAACACGAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAGAAAGATTGCAAAAACAATATGAGGAATACAAGCTTCAAAAGATGGGGAGAGAAGAAAAGCGGAAAGAAGAGAAGCAGAGAGAGCATAAATGCAAAAAGGAAGACAACGGTACAGTCGATGTCAAGGCGGTCGACCCTGACGATGGAAGAGCAAAAGTCCAACGAGTACATCAGAGGAACAAGAATGTTGGCGGGCAAAGAAGTGGCAAAACGAACAACTTCGACAATGGAAATTTCACAAGAAAGAGAAAGAAAAAGCTAAGAGGGAAAGGAGGAAAGAAATTTTGA

Coding sequence (CDS)

ATGTCAAAGTATGAATTTCCCAGGCTTCCACGGGCAGATATTGCATTGCTACTAGCACAGTGTCAAATTGCTGCTGTAACAGAGCGGGACCTTCTTCACCCGTCACCAGATTTGGTTTCAGATCTTTACACACGTTTAATGATCTACCTTGACTTGCTCCATGAGGAAGACCAAGGACAAGTTGAGTTTGCTGCCTTGGATCAGCTTGAGAACCCTGATCTGCATATGGACTCAGTTCGGATAATGAAGTTGCACAACAAAATAAAGCATGTCATTGCCTCTCTTGACTGTCCAAAAAAGTTTACATTGAAAGATTTGATAAAACCTGAAGGAGATAGGACCGAAATTTTCCTTAGTGCAATGTTGAACTTTTGCATTCACAAAGATGCCAAAATCACCCTCCATACTCCAGTTATGGATGAGCTGAGTACTCTTGGTGACCAGCAAAGGGAATGGGAGGTTAAGATTTCTCAGTTGAATGCAGAAATCGCAGAGTACAATGAAGCAAGAGAAAGGGAAATACCTTTTGTTCAAGAGATTGATGCTAAAGTGAAAGAACTACAACAGACTATTGGAGGACTTAACAATCAGCAGATGTCATTGCGTGCTTCTATCCGAAAGTTGAAAGACAAAGCTGGAGAAATGGATGAGAAGGTTGGAACTACACTGGAAACAATGCAACACGAGATCAAGGAAATGCAACAAGGGATCAAGAAAGTGCAACAAATCTTAGAAAGATTGCAAAAACAATATGAGGAATACAAGCTTCAAAAGATGGGGAGAGAAGAAAAGCGGAAAGAAGAGAAGCAGAGAGAGCATAAATGCAAAAAGGAAGACAACGGTACAGTCGATGTCAAGGCGGTCGACCCTGACGATGGAAGAGCAAAAGTCCAACGAGTACATCAGAGGAACAAGAATGTTGGCGGGCAAAGAAGTGGCAAAACGAACAACTTCGACAATGGAAATTTCACAAGAAAGAGAAAGAAAAAGCTAAGAGGGAAAGGAGGAAAGAAATTTTGA

Protein sequence

MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQVEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSAMLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEIDAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKVQQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDVKAVDPDDGRAKVQRVHQRNKNVGGQRSGKTNNFDNGNFTRKRKKKLRGKGGKKF

Homology

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BLAST of Lag0014700 vs. NCBI nr
Match: XP_038877491.1 (kinetochore protein NUF2 homolog [Benincasa hispida] >XP_038877492.1 kinetochore protein NUF2 homolog [Benincasa hispida])

HSP 1 Score: 402.5 bits (1033), Expect = 3.6e-108
Identity = 221/285 (77.54%), Postives = 237/285 (83.16%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKY+FPRLPRADIALLLA  QIAAVTERDLLHPS DLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYDFPRLPRADIALLLADYQIAAVTERDLLHPSLDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAALDQLENPDLHMDSV  MKL NKIKHVIASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  VEFAALDQLENPDLHMDSVSTMKLLNKIKHVIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVMDEL+T GDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMDELNTFGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK     C +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEKLAMQAC-QEKTGIVEV 281

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BLAST of Lag0014700 vs. NCBI nr
Match: KAG6588803.1 (Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-107
Identity = 216/285 (75.79%), Postives = 241/285 (84.56%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 58  MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 117

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 118 VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 177

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 178 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 237

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 238 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 297

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 298 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 338

[Close]

BLAST of Lag0014700 vs. NCBI nr
Match: XP_022927739.1 (probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927740.1 probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927741.1 probable kinetochore protein NUF2 [Cucurbita moschata] >KAG7022576.1 NUF2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-107
Identity = 216/285 (75.79%), Postives = 241/285 (84.56%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. NCBI nr
Match: XP_023529466.1 (probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529467.1 probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529468.1 probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-107
Identity = 216/285 (75.79%), Postives = 241/285 (84.56%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. NCBI nr
Match: XP_022989268.1 (probable kinetochore protein NUF2 [Cucurbita maxima] >XP_022989269.1 probable kinetochore protein NUF2 [Cucurbita maxima])

HSP 1 Score: 399.1 bits (1024), Expect = 3.9e-107
Identity = 215/285 (75.44%), Postives = 240/285 (84.21%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot
Match: Q8RXJ0 (Kinetochore protein NUF2 homolog OS=Arabidopsis thaliana OX=3702 GN=NUF2 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.9e-60
Identity = 124/267 (46.44%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MS YE+PRL R+DI   L   QIA+VTE DL  P+ D VS+LYTR++IYLD L EE++GQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           V+F AL+QLENPD H  S++ MKL+ K+K ++  LDCP   + KDL++PE  RTE F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LN+ ++KD+K+ L  P  +EL  L +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           +A +++L + I  LNNQQMSLRA+ +K+++K+ +MD ++      +   ++E      ++
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLVETVQENANLRSQI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKE 268
            Q  ++LQ   EE KL  +G  +K ++
Sbjct: 241 VQSPDKLQGALEEKKL-VLGETKKAEQ 266

[Close]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot
Match: Q10173 (Kinetochore protein nuf2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nuf2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 61/249 (24.50%), Postives = 120/249 (48.19%), Query Frame = 0


Query: 3   KYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-IYLDLLHEEDQGQV 62
           K+ FP L RA+I   +    I   T ++L  P+   V  LY   + +++ L  +  + +V
Sbjct: 4   KHTFPSLKRAEILECIDGLGI-PFTAKELDQPTSKAVIPLYEEFLDLFMGLTRQNLEEKV 63

Query: 63  EFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSAM 122
             +  D +EN ++  +S+R    +  +   + ++ C   FT++DL+KP+ +R ++ LSA+
Sbjct: 64  N-SLQDSVENFEIIHESLRFTVFYQILSQFMQNI-CFHDFTIQDLLKPDRNRLQLILSAV 123

Query: 123 LNFCIHK-------DAKITLHTPVMDELSTLGDQQREWEVKI-----SQLNAE-IAEYNE 182
           +NF   +       D  I     +++  + L  Q+++ E K+      +L +E I + NE
Sbjct: 124 INFAKLREERLQQFDDDIQKRESLLETYTLLDAQRKDLEEKVLLSQDRKLESEAIIKQNE 183

Query: 183 AREREIPFVQEIDAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQH 238
            R  E+ F   I+ K     +    +  +   +R    +LK +   +D  + +TLE    
Sbjct: 184 ERNEEM-FQSLIEDK-----RLCSQVRTEYDRIRMEASELKIRYHNVDSLMASTLE---- 239

[Close]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot
Match: P33895 (Kinetochore protein NUF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=NUF2 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.1e-06
Identity = 58/280 (20.71%), Postives = 127/280 (45.36%), Query Frame = 0


Query: 6   FPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-----IYLDLLHEEDQGQ 65
           FP L   ++ + L  C  A  T+ ++  P+ D +  LY +++     I ++ L      +
Sbjct: 8   FPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQE 67

Query: 66  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 125
                L Q EN ++++D++ ++ L NKI          + F + DL KPE  RT+  LSA
Sbjct: 68  TGDGHL-QEENENIYLDTLNVLVL-NKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSA 127

Query: 126 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 185
           ++N+   ++ ++      + ++ +L  Q R      + +  ++ +Y +     IP  QE+
Sbjct: 128 VVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQEL 187

Query: 186 D-------------AKVKELQQTIGGLNNQQMSLRASIRK----LKDKAGEMDEKVGTTL 245
                          K+ ++Q+T+    N     + SI K    L  +  E++      +
Sbjct: 188 QKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDKLI 247

Query: 246 ETMQHEIKEMQQGIKKVQQILERLQKQYEEYKLQKMGREE 264
           +    +++E+ +GIK++  +L + +K  ++   Q+   ++
Sbjct: 248 KISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQD 285

[Close]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot
Match: Q6CJ06 (Probable kinetochore protein NUF2 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=NUF2 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 1.7e-04
Identity = 61/263 (23.19%), Postives = 106/263 (40.30%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-----IYLDLLHE 60
           MSK  FP L  +++   L  C  +   E ++  PS   V  LY +++     +  D L  
Sbjct: 1   MSKDVFPLLELSELVTCLQSCDFSLAVEENISKPSSQYVITLYKQIIDTFMGVSPDTLLS 60

Query: 61  EDQGQVEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTE 120
            +       + D  +NP  + ++V+++ L NKI            F + DL KP+  RT 
Sbjct: 61  NEALFDNSGSNDIQQNP-AYTETVKVLAL-NKICFKFFQDIGVSDFNMMDLYKPDSLRTR 120

Query: 121 IFLSAMLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIP 180
             LSA++N+   ++ ++      M +           E  ++QL ++  +YN        
Sbjct: 121 RLLSAVVNYARFREERMFDCDKFMSKT----------EFLLNQLRSKFDDYN-------- 180

Query: 181 FVQEIDAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQ 240
                      LQQ I    N                 E++ + G T ET+Q + K + Q
Sbjct: 181 ----------YLQQQINKHRN-----------------EIELREGETFETLQQQNKHLDQ 216

Query: 241 GIKKVQQILERLQKQYEEYKLQK 259
            I +++ + E L   Y  YK +K
Sbjct: 241 QISRLKGLQETLNIDYNAYKSRK 216

[Close]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot
Match: Q757M3 (Probable kinetochore protein NUF2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=NUF2 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.2e-04
Identity = 66/288 (22.92%), Postives = 130/288 (45.14%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLM-----IYLDLLHE 60
           MS   FP L   ++   L +C  +  T +++  PS   V  LY +++     +  D L E
Sbjct: 1   MSSDNFPLLDIPELVTCLQECDFSLATIQNVERPSSQFVITLYKQIIDTFMGVSPDSLLE 60

Query: 61  EDQGQVEFAALDQLENPD---LHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGD 120
           + +  VE     + E  D   ++ D+++I+ L NKI +          F + DL KP+  
Sbjct: 61  DSRNFVENGDTSEQEVYDASLVYHDTLKILAL-NKICYKFFLDIGVSDFNVMDLYKPDPH 120

Query: 121 RTEIFLSAMLNFC-IHKDAKITL----HTPVMDELSTLGDQQREWEVKI---SQLNAEIA 180
           RT  FLSA+ N+    ++A + L    H   +  L       +E  +K    + L++ + 
Sbjct: 121 RTRRFLSAVANYARFREEAMLELDGDQHPDGVRYLEQTDKLLKELRLKFDANNLLHSRVQ 180

Query: 181 EYNEAREREIPFVQEIDAKVKELQQTIGGLNNQQMSLRASIRKLK-DKAGEMDEKVGTTL 240
           +Y E ++ +   ++ +++  K ++  +  LN  Q +L     + K +K   + E     L
Sbjct: 181 KYKEFQKSD--DLEALESDNKNMESELRHLNQVQETLTVDYERYKAEKQRHLSE-----L 240

Query: 241 ETMQHEIKEMQ------------------QGIKKVQQILERLQKQYEE 254
           ET+ +E+ E++                  +G++++  +LE+ Q Q E+
Sbjct: 241 ETLGYELIELESERDKLRKYSQTDAEVLRKGVRELSLLLEQQQVQLEK 280

[Close]

BLAST of Lag0014700 vs. ExPASy TrEMBL
Match: A0A6J1EIG6 (probable kinetochore protein NUF2 OS=Cucurbita moschata OX=3662 GN=LOC111434552 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 6.5e-108
Identity = 216/285 (75.79%), Postives = 241/285 (84.56%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. ExPASy TrEMBL
Match: A0A6J1JNW1 (probable kinetochore protein NUF2 OS=Cucurbita maxima OX=3661 GN=LOC111486390 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.9e-107
Identity = 215/285 (75.44%), Postives = 240/285 (84.21%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLAQCQIAAVTERDLLHP+PDLVSDLYTRLMIYLD LHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLAQCQIAAVTERDLLHPAPDLVSDLYTRLMIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAAL+Q ENPDLHMDSV+ MKL+N++KHVIASLDCPKKFTLKDLIKP+ DRTEIFLSA
Sbjct: 61  VEFAALEQFENPDLHMDSVQTMKLYNRVKHVIASLDCPKKFTLKDLIKPDPDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH PVM+EL+TL DQQREWEVKISQLNAEIAEYNEARERE+PFVQEI
Sbjct: 121 MLNFCIHKDAKINLHAPVMNELNTLDDQQREWEVKISQLNAEIAEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQMSLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQMSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEK-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. ExPASy TrEMBL
Match: A0A1S4E5E4 (probable kinetochore protein NUF2 OS=Cucumis melo OX=3656 GN=LOC103503319 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.4e-102
Identity = 210/285 (73.68%), Postives = 231/285 (81.05%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADIALLLA  QIAAVTERDLL PSPDLVSDLYT LMIYLDLLHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIALLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           +EFAALDQLENPDLHM SV  MKLHNKIKH IASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPEADRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LNF IHKDAK+  H PVM+EL T  DQQREWEVKISQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKISQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQ+SLRA+IRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRAAIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK     C +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEKLAMQAC-QEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. ExPASy TrEMBL
Match: A0A0A0KNL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G488740 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 1.2e-101
Identity = 209/285 (73.33%), Postives = 229/285 (80.35%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKYEFPRLPRADI LLLA  QIAAVTERDLL PSPDLVSDLYT LMIYLDLLHEEDQGQ
Sbjct: 1   MSKYEFPRLPRADIVLLLADNQIAAVTERDLLQPSPDLVSDLYTHLMIYLDLLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           +EFAALDQLENPDLHM SV  MKLHNKIKH IASLDCPKKFTLKDLIKPE DRTEIFLSA
Sbjct: 61  IEFAALDQLENPDLHMVSVPTMKLHNKIKHFIASLDCPKKFTLKDLIKPETDRTEIFLSA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LNF IHKDAK+  H PVM+EL T  DQQREWEVK SQLNAEI EYNEARERE+PFVQEI
Sbjct: 121 ILNFGIHKDAKMNFHAPVMNELDTFADQQREWEVKTSQLNAEITEYNEAREREMPFVQEI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL QTIGGLNNQQ+SLRASIRKLK+KAGEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQTIGGLNNQQVSLRASIRKLKEKAGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K+ REE +  EK     C +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKLAREEAKNAEKLAMQAC-QEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. ExPASy TrEMBL
Match: A0A6J1DWL1 (probable kinetochore protein NUF2 OS=Momordica charantia OX=3673 GN=LOC111024175 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 5.0e-100
Identity = 207/285 (72.63%), Postives = 232/285 (81.40%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MSKY   RLP ADIALLLAQ QIAAVTE DLLHPSPDLVSDLYTRL+IYLD LHEEDQGQ
Sbjct: 1   MSKYGLLRLPPADIALLLAQFQIAAVTEGDLLHPSPDLVSDLYTRLLIYLDFLHEEDQGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           VEFAALDQLENPDLHMDSVRIMKL+ K+KHVIASLDC KKFTLKDLIKP+ DRTEIFLS 
Sbjct: 61  VEFAALDQLENPDLHMDSVRIMKLNLKLKHVIASLDCSKKFTLKDLIKPDTDRTEIFLST 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           MLNFCIHKDAKI LH P+++E++TLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQ+I
Sbjct: 121 MLNFCIHKDAKIVLHAPIINEVNTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQDI 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           DAKVKEL Q IGGLNNQQMSLRASIRKLK+K GEMDEK+      +   ++E      K+
Sbjct: 181 DAKVKELHQIIGGLNNQQMSLRASIRKLKEKVGEMDEKISNAEFLLVQSVQENANLRSKI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKEEKQREHKCKKEDNGTVDV 286
            Q  ++LQ+  EE   +K  REE +  E+    +  +E  G V+V
Sbjct: 241 VQSPDKLQRALEE---KKSAREETKNAER-LAMQAFQEKTGIVEV 281

[Close]

BLAST of Lag0014700 vs. TAIR 10
Match: AT1G61000.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plasma membrane; EXPRESSED IN: cultured cell; CONTAINS InterPro DOMAIN/s: Kinetochore protein Nuf2 (InterPro:IPR005549); Has 50972 Blast hits to 29793 proteins in 2070 species: Archae - 902; Bacteria - 7404; Metazoa - 23628; Fungi - 4156; Plants - 2312; Viruses - 158; Other Eukaryotes - 12412 (source: NCBI BLink). )

HSP 1 Score: 233.8 bits (595), Expect = 2.0e-61
Identity = 124/267 (46.44%), Postives = 188/267 (70.41%), Query Frame = 0


Query: 1   MSKYEFPRLPRADIALLLAQCQIAAVTERDLLHPSPDLVSDLYTRLMIYLDLLHEEDQGQ 60
           MS YE+PRL R+DI   L   QIA+VTE DL  P+ D VS+LYTR++IYLD L EE++GQ
Sbjct: 1   MSAYEYPRLSRSDIITALKDAQIASVTETDLKTPTSDFVSELYTRILIYLDALDEEEKGQ 60

Query: 61  VEFAALDQLENPDLHMDSVRIMKLHNKIKHVIASLDCPKKFTLKDLIKPEGDRTEIFLSA 120
           V+F AL+QLENPD H  S++ MKL+ K+K ++  LDCP   + KDL++PE  RTE F+SA
Sbjct: 61  VDFEALEQLENPDHHATSMQAMKLYCKVKDMLEMLDCPLPISFKDLLRPESSRTEFFISA 120

Query: 121 MLNFCIHKDAKITLHTPVMDELSTLGDQQREWEVKISQLNAEIAEYNEAREREIPFVQEI 180
           +LN+ ++KD+K+ L  P  +EL  L +Q+++ E K++QLNAEI E++EA ER++PFVQE+
Sbjct: 121 LLNYGLYKDSKMDLIRPKAEELGLLDEQRKQCEAKVAQLNAEIGEFDEAVERDLPFVQEL 180

Query: 181 DAKVKELQQTIGGLNNQQMSLRASIRKLKDKAGEMDEKVGTTLETMQHEIKEMQQGIKKV 240
           +A +++L + I  LNNQQMSLRA+ +K+++K+ +MD ++      +   ++E      ++
Sbjct: 181 EANIEQLNKKILELNNQQMSLRATFQKMREKSTQMDNEISKAEFDLVETVQENANLRSQI 240

Query: 241 QQILERLQKQYEEYKLQKMGREEKRKE 268
            Q  ++LQ   EE KL  +G  +K ++
Sbjct: 241 VQSPDKLQGALEEKKL-VLGETKKAEQ 266

The following BLAST results are available for this feature:

BLAST of Lag0014700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877491.1	3.6e-108	77.54	kinetochore protein NUF2 homolog [Benincasa hispida] >XP_038877492.1 kinetochore...	[more]
KAG6588803.1	1.4e-107	75.79	Kinetochore protein NUF2-like protein, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022927739.1	1.4e-107	75.79	probable kinetochore protein NUF2 [Cucurbita moschata] >XP_022927740.1 probable ...	[more]
XP_023529466.1	1.4e-107	75.79	probable kinetochore protein NUF2 [Cucurbita pepo subsp. pepo] >XP_023529467.1 p...	[more]
XP_022989268.1	3.9e-107	75.44	probable kinetochore protein NUF2 [Cucurbita maxima] >XP_022989269.1 probable ki...	[more]

BLAST of Lag0014700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXJ0	2.9e-60	46.44	Kinetochore protein NUF2 homolog OS=Arabidopsis thaliana OX=3702 GN=NUF2 PE=1 SV...	[more]
Q10173	1.4e-06	24.50	Kinetochore protein nuf2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
P33895	4.1e-06	20.71	Kinetochore protein NUF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c...	[more]
Q6CJ06	1.7e-04	23.19	Probable kinetochore protein NUF2 OS=Kluyveromyces lactis (strain ATCC 8585 / CB...	[more]
Q757M3	2.2e-04	22.92	Probable kinetochore protein NUF2 OS=Ashbya gossypii (strain ATCC 10895 / CBS 10...	[more]

BLAST of Lag0014700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EIG6	6.5e-108	75.79	probable kinetochore protein NUF2 OS=Cucurbita moschata OX=3662 GN=LOC111434552 ...	[more]
A0A6J1JNW1	1.9e-107	75.44	probable kinetochore protein NUF2 OS=Cucurbita maxima OX=3661 GN=LOC111486390 PE...	[more]
A0A1S4E5E4	1.4e-102	73.68	probable kinetochore protein NUF2 OS=Cucumis melo OX=3656 GN=LOC103503319 PE=3 S...	[more]
A0A0A0KNL7	1.2e-101	73.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G488740 PE=3 SV=1	[more]
A0A6J1DWL1	5.0e-100	72.63	probable kinetochore protein NUF2 OS=Momordica charantia OX=3673 GN=LOC111024175...	[more]

BLAST of Lag0014700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61000.1	2.0e-61	46.44	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: mitosis; LOCATED IN: plas...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 177..197
None	No IPR available	COILS	Coil	Coil	coord: 149..169
None	No IPR available	COILS	Coil	Coil	coord: 223..279
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 260..299
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 303..320
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 260..339
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 325..339
None	No IPR available	PANTHER	PTHR21650	MEMBRALIN/KINETOCHORE PROTEIN NUF2	coord: 1..273
None	No IPR available	PANTHER	PTHR21650:SF2	KINETOCHORE PROTEIN NUF2	coord: 1..273
IPR038275	Nuf2, N-terminal domain superfamily	GENE3D	1.10.418.60	Ncd80 complex, Nuf2 subunit	coord: 1..164 e-value: 3.1E-36 score: 126.8
IPR005549	Kinetochore protein Nuf2, N-terminal	PFAM	PF03800	Nuf2	coord: 3..141 e-value: 6.0E-28 score: 97.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0014700.1	Lag0014700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051315	attachment of mitotic spindle microtubules to kinetochore
biological_process	GO:0051301	cell division
biological_process	GO:0051383	kinetochore organization
biological_process	GO:0045132	meiotic chromosome segregation
biological_process	GO:0007052	mitotic spindle organization
cellular_component	GO:0031262	Ndc80 complex
cellular_component	GO:0000776	kinetochore
molecular_function	GO:0044877	protein-containing complex binding