Lag0014653 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0014653 vs. NCBI nr
Match: KAG7022562.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 721/803 (89.79%), Postives = 747/803 (93.03%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 565  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 624

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 625  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 684

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ------NNMNNMQ 412
             GQLPASHMQSIQQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ      NNMNNMQ
Sbjct: 685  QGQLPASHMQSIQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMQ 744

Query: 413  HSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSS 472
            H+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQRANNSS
Sbjct: 745  HNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRANNSS 804

Query: 473  LPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LKQHM 532
            L SQNGVNALQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LKQ M
Sbjct: 805  LASQNGVNALQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLKQQM 864

Query: 533  L------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGM 592
            L      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIE+NDLKMRQG+AAK GM
Sbjct: 865  LQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEMNDLKMRQGVAAKPGM 924

Query: 593  FQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTP 652
            FQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + GTP
Sbjct: 925  FQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRIGTP 984

Query: 653  LQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLAIGT 712
            LQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLAIGT
Sbjct: 985  LQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLAIGT 1044

Query: 713  PGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIG 772
            PGISASPLLAEFSG DGAYA ALPTVSGKS+VTEQPLERLIKAVKSMSPKAL+ASVSGIG
Sbjct: 1045 PGISASPLLAEFSGTDGAYAIALPTVSGKSSVTEQPLERLIKAVKSMSPKALNASVSGIG 1104

Query: 773  SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPL 832
            SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSAMPL
Sbjct: 1105 SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSAMPL 1164

Query: 833  NVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVV 892
            NVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDTVVV
Sbjct: 1165 NVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDTVVV 1224

Query: 893  ISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP 952
            ISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP
Sbjct: 1225 ISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP 1284

Query: 953  NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEY 1012
            NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAVVSEY
Sbjct: 1285 NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAVVSEY 1344

Query: 1013 AQQSGGGSFCSKYGAWENCLSGA 1023
            AQQSGGGSFCSKYG WENCLS A
Sbjct: 1345 AQQSGGGSFCSKYGTWENCLSAA 1365

BLAST of Lag0014653 vs. NCBI nr
Match: KAG7022562.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 101.3 bits (251), Expect = 5.1e-17
Identity = 161/504 (31.94%), Postives = 234/504 (46.43%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIYTAAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYTAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQA 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSSNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKYQQ 277

Query: 399 SVAPVQ--QNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSLSSLQQVAAG 458
              P Q  Q+++   Q + ++     + QQ++   MQP  S+L+  Q +S+    Q    
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMAPNQLPSSQQSV---MQPSLSNLQQNQQSSIQQPTQSMLQ 337

Query: 459 SLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQS--- 518
              Q      Q++   S+  Q    + Q ++S  Q    I Q  +        L+Q+   
Sbjct: 338 QPPQPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQQSNSSNMQQNSLIQNSVG 397

Query: 519 --QQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLS-TQMPTHQMSHLNQIE 578
             QQ L QQ +    Q     +Q  P   QQQ Q  Q   QQ S + M   Q+    Q  
Sbjct: 398 DMQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQLMNQQSSLSNMQQPQLG--PQSN 457

Query: 579 INDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSF--PISPPIFQATSPQVTQNSS 638
           ++ L+ +Q    + G   ++ + Q   +  QQ K       P +P    +T  Q  Q  S
Sbjct: 458 VSGLQQQQMHGTQSGN-SNMQSNQHGVHMMQQNKVQMQQQPPQNPSNLLSTQGQQGQLQS 510

Query: 639 PQVDQQSLLSSIPKTGTPLQSASS 646
                Q L+S IP     +Q   S
Sbjct: 518 ----SQQLMSQIPLQSAQVQQQVS 510

HSP 2 Score: 1288.9 bits (3334), Expect = 0.0e+00
Identity = 718/806 (89.08%), Postives = 745/806 (92.43%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 564  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 623

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 624  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 683

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 684  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 743

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 744  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 803

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 804  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 863

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 864  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 923

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 924  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 983

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 984  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 1043

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 1044 IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 1103

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 1104 GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1163

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1164 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1223

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1224 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1283

Query: 953  NYPNCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVV 1012
            NYPNCSPILLDKFP+EVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAVV
Sbjct: 1284 NYPNCSPILLDKFPIEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAVV 1343

Query: 1013 SEYAQQSGGGSFCSKYGAWENCLSGA 1023
            SEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1344 SEYAQQSGGGSFCSKYGAWENCLSAA 1367

BLAST of Lag0014653 vs. NCBI nr
Match: XP_022988664.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-16
Identity = 161/512 (31.45%), Postives = 233/512 (45.51%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIY AAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYAAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQA 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSTNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKFQQ 277

Query: 399 SVAPVQ--QNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSLSSLQQVAAG 458
              P Q  Q+++   Q + +      + QQ++   MQP  S+L+  Q +S+    Q    
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMPPNQLQSSQQSV---MQPSLSNLQQNQQSSIQQPTQSMLQ 337

Query: 459 SLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQS--- 518
              Q      Q++   S+  Q    + Q ++S  Q    I Q  +        L+Q+   
Sbjct: 338 QPPQPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQQSNSSNMQQNSLIQNSVG 397

Query: 519 --QQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEI 578
             QQ L QQ +    Q     +Q  P   QQQ Q  QQ   Q S+     Q     Q  +
Sbjct: 398 DMQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQQLMNQQSSLSNLQQPQLGPQSNV 457

Query: 579 NDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSF--PISPPIFQATSPQVTQNSSP 638
           + L+ +Q    + G   ++ + Q   +  QQ K       P +P    +T  Q  Q  S 
Sbjct: 458 SGLQQQQMHGTQSGN-SNMQSNQHGVHMMQQNKVQMQQQPPQNPSNLLSTQGQQGQLQS- 517

Query: 639 QVDQQSLLSSIPKTGTPLQSASSPFVVPSPST 655
               Q L+S IP     +Q   S    P  +T
Sbjct: 518 ---SQQLMSQIPLQSAQVQQQVSLQQQPQSNT 520

HSP 2 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 720/801 (89.89%), Postives = 747/801 (93.26%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QAN GDWQEEIYQKIKAMKELYFFELKEMYQKILPK++QFDSLPQQPK
Sbjct: 569  STSLDSTAQTG-QANAGDWQEEIYQKIKAMKELYFFELKEMYQKILPKLNQFDSLPQQPK 628

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
             EQLNKLKAFK ILERLLAFLQV KSNIV+GFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 629  LEQLNKLKAFKSILERLLAFLQVPKSNIVLGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 688

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSLST 412
            PGQLPASHM SIQQSQ+QMT LQSPENQIN QLHSANMQ SVAPVQQNNMNNMQH+SLS+
Sbjct: 689  PGQLPASHMHSIQQSQSQMTSLQSPENQINSQLHSANMQCSVAPVQQNNMNNMQHNSLSS 748

Query: 413  FSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNG 472
            FSGSA QQNMT PMQPGS LESGQGNSLSSLQQVAAGSLQQNPAN+SQRAN+SSL SQNG
Sbjct: 749  FSGSAAQQNMTSPMQPGSGLESGQGNSLSSLQQVAAGSLQQNPANTSQRANSSSLSSQNG 808

Query: 473  VNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQM-QLKQHML----- 532
            VN LQ NIS LQSNPN+LQHQHLKQQDPQQL+QS Q LKQQ+QQR + QLKQ ML     
Sbjct: 809  VNPLQQNISALQSNPNMLQHQHLKQQDPQQLMQSPQQLKQQLQQRHIHQLKQQMLQQHQQ 868

Query: 533  -QQQPQLHQQQSQLHQQGKQQLST--QMPTHQMSHLNQI-EINDLKMRQGLAAKQGMFQH 592
             QQQPQ HQQQSQLHQQGKQQL T  QM  HQMSHLN I EINDLKMRQGLA K GMFQH
Sbjct: 869  QQQQPQSHQQQSQLHQQGKQQLPTQMQMQAHQMSHLNPINEINDLKMRQGLAVKPGMFQH 928

Query: 593  LPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTPLQS 652
            LPATQRSAY+H Q+KPGTSFPISPPIFQA SPQV QNSSPQVDQQSLLSSI KTGTPLQS
Sbjct: 929  LPATQRSAYSHPQLKPGTSFPISPPIFQAASPQVAQNSSPQVDQQSLLSSITKTGTPLQS 988

Query: 653  ASSPFVVPSPST-PAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLAIGTPG 712
            ASSPFVVPSPST P APSP+PGDSEKP+S V+S T+AGNTGQQTSV GTQAQSLAIGTPG
Sbjct: 989  ASSPFVVPSPSTSPVAPSPIPGDSEKPMSAVSSLTNAGNTGQQTSVSGTQAQSLAIGTPG 1048

Query: 713  ISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV 772
            ISASPLLAEFSG DG+Y NALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV
Sbjct: 1049 ISASPLLAEFSGTDGSYVNALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV 1108

Query: 773  VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNV 832
            VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTKKM+R+TSAMPLNV
Sbjct: 1109 VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGLNGTKKMRRYTSAMPLNV 1168

Query: 833  VSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVIS 892
            VSSAGSVNDV KPLTGAETSDLESTATSSVKRPRVEA+H+LLEEIREINQRLIDTVVVIS
Sbjct: 1169 VSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRVEANHVLLEEIREINQRLIDTVVVIS 1228

Query: 893  DEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYPNC 952
            DEVVDPSAL AAADGSEGTIVKCSFSAVALSP+LKSQY SAQMSPIQPLRLLVPTNYPNC
Sbjct: 1229 DEVVDPSALVAAADGSEGTIVKCSFSAVALSPNLKSQYASAQMSPIQPLRLLVPTNYPNC 1288

Query: 953  SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEYAQ 1012
            SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLG+IARTWD+CARAVVSEYAQ
Sbjct: 1289 SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGEIARTWDVCARAVVSEYAQ 1348

Query: 1013 QSGGGSFCSKYGAWENCLSGA 1023
            QSGGGSFCSKYGAWENCLS A
Sbjct: 1349 QSGGGSFCSKYGAWENCLSAA 1368

BLAST of Lag0014653 vs. NCBI nr
Match: XP_022154411.1 (mediator of RNA polymerase II transcription subunit 15a [Momordica charantia])

HSP 1 Score: 85.1 bits (209), Expect = 3.8e-12
Identity = 201/653 (30.78%), Postives = 277/653 (42.42%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTT-- 218
           METL R LPVSG++GLSELRKIAVRFEEKIYTAAT+QSDYLRKISLKMLTMETKSQ++  
Sbjct: 38  METLKRHLPVSGNEGLSELRKIAVRFEEKIYTAATSQSDYLRKISLKMLTMETKSQSSMG 97

Query: 219 PMVPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQK 278
             +PSNS+VP NKP+D+AS    +Q             I Q    +         +  Q+
Sbjct: 98  NPLPSNSMVPSNKPLDTASQSMPSQA------------INQSTVPLSS----NQPQSRQQ 157

Query: 279 ILPKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL--QVSKSNIVIGFKDKIGHYEKQ 338
           +L +  Q +++  Q  S   + +     +    +  +  Q S    V G    +G+   Q
Sbjct: 158 LLSQNIQ-NTIAPQSSSSLPSAVPPVTGLAPSSMPTMVGQSSSMQSVSGVSQNVGNTVGQ 217

Query: 339 IVS---FLHSNRP---RNPVSTLQPGQLPASHMQSIQ-QSQAQMTPLQSPENQINPQLHS 398
            V    F +S RP   R  VS  Q  Q  +      Q Q Q Q+   +  +  ++ QL  
Sbjct: 218 GVPSNVFPNSQRPIQGRQVVSQQQQQQSQSQQQYLYQHQLQQQLIKQKYQQGSMSHQLMQ 277

Query: 399 ANM-QASVAPVQQNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSL----- 458
            +M Q     +Q N + + Q S L T S   P       MQP  S+L+  Q + +     
Sbjct: 278 PHMSQQQQNLIQPNQLQSSQQSVLQTSSVMQPS-----VMQPSLSNLQQSQQSPIQQPTQ 337

Query: 459 SSLQQVAAGSLQQNPANSSQRA--------NNSSLPSQNGVNAL--QPNISPLQSNPNIL 518
           S LQQ +   L+Q   +    A          + LPSQ     +  QPN S +Q N  I 
Sbjct: 338 SMLQQPSQPVLRQQQQSQPHAAMHQQPTMPQQTMLPSQQQQQLISQQPNSSNMQQNSLIG 397

Query: 519 QHQHLKQQDPQQLLQSQQHLKQ----QMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQ 578
           Q   L     Q   QS+ H +Q     MQ    Q +Q ++ QQ  L    S L QQ +QQ
Sbjct: 398 QQSSLGDMQQQLPQQSRSHGQQSNLSNMQSPPSQQQQQLMTQQNNL----SNLQQQQQQQ 457

Query: 579 LSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSFPISP 638
           L    P   +S L Q           +   Q     L   Q      QQ  P TS  +S 
Sbjct: 458 LG---PQSNVSGLQQQIHGTQSGNSNMQTNQHSVHML--HQNKVQMQQQPPPNTSNLLS- 517

Query: 639 PIFQATSPQVTQNSSPQVD-QQSLLSSIPKTGT------PLQSASSPFVVPSPSTP---A 698
                     TQ    Q+   Q L+S IP   T      PLQ    P  V S        
Sbjct: 518 ----------TQGQQGQLQPSQQLMSQIPLQSTQVQQQVPLQQQQQPPNVMSHELQQRLQ 577

Query: 699 APSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSL--AIGTPGISASPLLAE---- 753
                PG   +P + +       ++  Q ++P T + SL     T   +A     E    
Sbjct: 578 VSGQAPGSLLQPQNVMDQQKQVYHS--QRALPETSSTSLDSTAQTGQANAGDWQEEIYQK 637

HSP 2 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 718/807 (88.97%), Postives = 745/807 (92.32%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 564  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 623

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 624  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 683

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 684  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 743

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 744  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 803

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 804  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 863

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 864  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 923

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 924  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 983

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 984  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 1043

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 1044 IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 1103

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 1104 GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1163

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1164 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1223

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1224 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1283

Query: 953  NYPNCSPILLDKFPVEV-SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAV 1012
            NYPNCSPILLDKFP+EV SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAV
Sbjct: 1284 NYPNCSPILLDKFPIEVSSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAV 1343

Query: 1013 VSEYAQQSGGGSFCSKYGAWENCLSGA 1023
            VSEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1344 VSEYAQQSGGGSFCSKYGAWENCLSAA 1368

BLAST of Lag0014653 vs. NCBI nr
Match: XP_022988663.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 100.1 bits (248), Expect = 1.1e-16
Identity = 161/512 (31.45%), Postives = 233/512 (45.51%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIY AAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYAAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQA 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSTNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKFQQ 277

Query: 399 SVAPVQ--QNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSLSSLQQVAAG 458
              P Q  Q+++   Q + +      + QQ++   MQP  S+L+  Q +S+    Q    
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMPPNQLQSSQQSV---MQPSLSNLQQNQQSSIQQPTQSMLQ 337

Query: 459 SLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQS--- 518
              Q      Q++   S+  Q    + Q ++S  Q    I Q  +        L+Q+   
Sbjct: 338 QPPQPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQQSNSSNMQQNSLIQNSVG 397

Query: 519 --QQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEI 578
             QQ L QQ +    Q     +Q  P   QQQ Q  QQ   Q S+     Q     Q  +
Sbjct: 398 DMQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQQLMNQQSSLSNLQQPQLGPQSNV 457

Query: 579 NDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSF--PISPPIFQATSPQVTQNSSP 638
           + L+ +Q    + G   ++ + Q   +  QQ K       P +P    +T  Q  Q  S 
Sbjct: 458 SGLQQQQMHGTQSGN-SNMQSNQHGVHMMQQNKVQMQQQPPQNPSNLLSTQGQQGQLQS- 517

Query: 639 QVDQQSLLSSIPKTGTPLQSASSPFVVPSPST 655
               Q L+S IP     +Q   S    P  +T
Sbjct: 518 ---SQQLMSQIPLQSAQVQQQVSLQQQPQSNT 520

HSP 2 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 718/807 (88.97%), Postives = 745/807 (92.32%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 459  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 518

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 519  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 578

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 579  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 638

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 639  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 698

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 699  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 758

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 759  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 818

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 819  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 878

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 879  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 938

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 939  IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 998

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 999  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1058

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1059 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1118

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1119 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1178

Query: 953  NYPNCSPILLDKFPVEV-SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAV 1012
            NYPNCSPILLDKFP+EV SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAV
Sbjct: 1179 NYPNCSPILLDKFPIEVSSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAV 1238

Query: 1013 VSEYAQQSGGGSFCSKYGAWENCLSGA 1023
            VSEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1239 VSEYAQQSGGGSFCSKYGAWENCLSAA 1263

BLAST of Lag0014653 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 7.0e-211
Identity = 464/799 (58.07%), Postives = 575/799 (71.96%), Query Frame = 0

Query: 231  MDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQ 290
            M S+SLDSTAQT + ANGGDWQEE+YQKIK+MKE Y  +L E+YQ++  K+ Q DS+PQQ
Sbjct: 574  MPSSSLDSTAQT-ESANGGDWQEEVYQKIKSMKETYLPDLNEIYQRVAAKLQQ-DSMPQQ 633

Query: 291  PKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVST 350
             +S+QL KL+ FK +LER++ FL VSKSNI+   KDK+ +YEKQI+ FL+ +RPR PV  
Sbjct: 634  QRSDQLEKLRQFKTMLERMIQFLSVSKSNIMPALKDKVAYYEKQIIGFLNMHRPRKPV-- 693

Query: 351  LQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSL 410
             Q GQLP S MQ +QQ Q+Q    QS +NQ NPQ+ S +MQ +    QQ++M NMQ + L
Sbjct: 694  -QQGQLPQSQMQPMQQPQSQTVQDQSHDNQTNPQMQSMSMQGAGPRAQQSSMTNMQSNVL 753

Query: 411  STFSG-SAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPS 470
            S+  G SAPQQN+   + P SSLESGQGN+L++ QQVA GS+QQ   N+SQ  NNSS  +
Sbjct: 754  SSRPGVSAPQQNIPSSI-PASSLESGQGNTLNNGQQVAMGSMQQ---NTSQLVNNSSASA 813

Query: 471  QNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQLKQHMLQQQ 530
            Q+G++ LQ N++  Q + ++LQHQHLKQQ  QQ+   QQ  ++QMQQ+Q+Q +Q   QQQ
Sbjct: 814  QSGLSTLQSNVNQPQLSSSLLQHQHLKQQQDQQMQLKQQFQQRQMQQQQLQARQQQQQQQ 873

Query: 531  PQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMF-QHLPATQR 590
             Q  QQ +QL Q                     ++NDL  RQG+   +GMF QH    QR
Sbjct: 874  LQARQQAAQLQQMN-------------------DMNDLTSRQGMNVSRGMFQQHSMQGQR 933

Query: 591  SAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTPLQSASSPFV 650
            + Y  QQ+KPG     SP + Q  SPQ++Q+ SPQVDQ+   +++ K GTPLQ A+SPFV
Sbjct: 934  ANYPLQQLKPGA--VSSPQLLQGASPQMSQHLSPQVDQK---NTVNKMGTPLQPANSPFV 993

Query: 651  VPSP-STPAAPSPMPGDSEKPISTVTSHTHAGNTGQQ--TSVPGTQAQSLAIGTPGISAS 710
            VPSP STP APSPM  DSEKP S+  S    GN  +Q  T + G   QSLAIGTPGISAS
Sbjct: 994  VPSPSSTPLAPSPMQVDSEKPGSSSLS---MGNIARQQATGMQGV-VQSLAIGTPGISAS 1053

Query: 711  PLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSVVSMI 770
            PLL EF+  DG   N+    SGK + TE P+ERLI+AVKS+SP+ALS++VS IGSVVSM+
Sbjct: 1054 PLLQEFTSPDGNILNSSTITSGKPSATELPIERLIRAVKSISPQALSSAVSDIGSVVSMV 1113

Query: 771  DRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNVVSSA 830
            DRIAGSAPGNGSRA+VGEDLVAMTKCRLQARNF++ +G   TKKMKRHT+AMPL+V S  
Sbjct: 1114 DRIAGSAPGNGSRASVGEDLVAMTKCRLQARNFMTQEGMMATKKMKRHTTAMPLSVASLG 1173

Query: 831  GSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVISD--E 890
            GSV D  K   G+ETSDLESTATS  K+ R E  H LLEEI+EINQRLIDTVV ISD  +
Sbjct: 1174 GSVGDNYKQFAGSETSDLESTATSDGKKARTETEHALLEEIKEINQRLIDTVVEISDDED 1233

Query: 891  VVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYPNCSP 950
              DPS +A ++ G EGT V+ SF AV+LSP+LK+  +S QMSPIQPLRLLVP +YPN SP
Sbjct: 1234 AADPSEVAISSIGCEGTTVRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSP 1293

Query: 951  ILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEYAQQS 1010
             LLDK PVE SKE EDLS KA +RF+I LR+LSQPMSL DIA+TWD CARAV+ EYAQQ 
Sbjct: 1294 SLLDKLPVETSKENEDLSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQF 1335

Query: 1011 GGGSFCSKYGAWENCLSGA 1023
            GGG+F SKYG WE  ++ +
Sbjct: 1354 GGGTFSSKYGTWEKYVAAS 1335

HSP 2 Score: 63.2 bits (152), Expect = 2.0e-08
Identity = 125/430 (29.07%), Postives = 182/430 (42.33%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL + LP SG +G++ELR+IA RFEEKI++ A NQ+DYLRKIS+KMLTMETKSQ    
Sbjct: 39  METLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQTDYLRKISMKMLTMETKSQNA-- 98

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
             S++ +P     +  S+DS      Q   G       Q  + +        + M     
Sbjct: 99  AGSSAAIPAAN--NGTSIDSIPTNQGQLLPGSLSTNQSQAPQPLLS------QTMQNNTA 158

Query: 279 PKVHQFDSLP-QQPKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVS 338
             +    +LP   P    +        + +        +  N+    +D  G +      
Sbjct: 159 SGMTGSTALPSSMPPVSSITNNNTTSVVNQ------NANMQNVAGMLQDSSGQHGLSSNM 218

Query: 339 FLHSNRP---RNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASV 398
           F    R    R    + Q  Q P  + Q +QQ   +           N  L S   Q   
Sbjct: 219 FSGPQRQMLGRPHAMSSQQQQQPYLYQQQLQQQLLKQNFQSGNVPNPNSLLPSHIQQQQQ 278

Query: 399 APVQQNNMNNMQHSSLSTFS------GSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAA 458
             +Q N +++ Q   + T +       SAP Q +    Q    L S Q  + S L+Q  +
Sbjct: 279 NVLQPNQLHSSQQPGVPTSATQPSTVNSAPLQGLHTNQQSSPQL-SSQQTTQSMLRQHQS 338

Query: 459 GSLQQNPAN---SSQRANNSSLPSQNGVNALQPNISPLQSNP-NILQHQHLKQQDPQQLL 518
             L+Q+P +   S      SSLP Q        +ISPLQ  P  +++ Q       QQ  
Sbjct: 339 SMLRQHPQSQQASGIHQQQSSLPQQ--------SISPLQQQPTQLMRQQAANSSGIQQKQ 398

Query: 519 QSQQH----LKQQMQQR-------QMQLKQHMLQQQP-QLHQQQSQLHQQGKQQLSTQMP 563
              QH    ++QQ QQR        M ++Q   QQQP Q  QQQ +     +QQL +Q  
Sbjct: 399 MMGQHVVGDMQQQHQQRLLNQQNNVMNIQQQQSQQQPLQQPQQQQKQQPPAQQQLMSQQN 443

BLAST of Lag0014653 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.6e-74
Identity = 278/803 (34.62%), Postives = 395/803 (49.19%), Query Frame = 0

Query: 234  ASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPKS 293
            AS DST +T    N G+WQEE YQKIK +KE+    L  M+Q++  K+ + +SLP QP  
Sbjct: 294  ASQDSTGKT-VNVNAGNWQEETYQKIKKLKEMCLPVLSLMHQRVAEKLRETESLPPQPMQ 353

Query: 294  EQ-LNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 353
             Q + KLKA K  +E L+ FL V +S++    +DK   YE  I+ F  S       +  Q
Sbjct: 354  AQWIEKLKAGKLSMEHLMFFLNVHRSSVSEKHRDKFSQYEYHILKFTKSQTMVLRPTQQQ 413

Query: 354  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSLST 413
             GQ P S  Q+  Q+       QSP+  ++  L+    ++ + P  QN     + SSL  
Sbjct: 414  QGQFPPS--QTAMQT-------QSPQVHVSQSLYKEQRRSRLMPSSQN-----EASSLLQ 473

Query: 414  FSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNG 473
                   ++  I M       +  GN       V   S++QNP                 
Sbjct: 474  IRPKLDPRDENIIM-------ASSGN-------VMLPSVKQNP----------------- 533

Query: 474  VNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQLKQHMLQQQPQL 533
              A+  NIS +QS                  LQ Q    ++   RQMQ      QQQP  
Sbjct: 534  -RAVNTNISSVQS------------------LQKQ----KRFHHRQMQ------QQQP-- 593

Query: 534  HQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYA 593
                    QQG  Q       HQM      E+ND++MR+ +  K  + +   ++ +    
Sbjct: 594  --------QQGNHQ-------HQM---QTNEMNDVRMRERVNIKARLLEQQVSSSQRQVP 653

Query: 594  HQQMKPGTSFPISPPIFQATSPQVTQNSSPQ-VDQQSLLSSIPKTGTPLQSASSPFVVPS 653
             Q+                +S Q+  +SSPQ VDQ  L ++I KTGTPL S+ S FV P 
Sbjct: 654  KQES-------------NVSSSQIQNHSSPQLVDQHILPATINKTGTPLNSSGSAFVAP- 713

Query: 654  PSTPAAPSPMPGDSEKPIST---VTSHTHAGNTGQQTSVPGTQAQSLAIGTPGISASPLL 713
                 APSP+PGDSE PIS    V+      +T   +S  GTQ             +PLL
Sbjct: 714  -----APSPVPGDSEMPISVESPVSGVDEINSTLDSSSKLGTQ------------ETPLL 773

Query: 714  AEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSVVSMIDRI 773
                            V     +TE+P++RLIKA ++ SPK+L+ SVS I SV+SM+D I
Sbjct: 774  ---------------FVPPPEPITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMI 833

Query: 774  AGSAPGN-GSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNVVSSAGS 833
             GS P + GSRA +GEDL   T      RNF +H+  N +K+MKR  + +P ++ S    
Sbjct: 834  GGSFPSSGGSRAGLGEDLSERT------RNFTTHEETNLSKRMKRSINIVPPDMSSQI-- 893

Query: 834  VNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVISDEVVDP 893
              D  + L+  E S++ ST +S +K   +   + LL+EI+E N RL++TVV I DE    
Sbjct: 894  --DSYEQLSSLE-SEVVSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDE---- 932

Query: 894  SALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQM----------SPIQPLRLLVPTN 953
                     S GTIV C+++ VALS + K  Y S ++          + IQPLRLL P +
Sbjct: 954  --------DSLGTIVTCTYAPVALSATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMD 932

Query: 954  YPNCSPILLDKFPVEVS-KEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVV 1013
            YP  SPI+L++   + S  +YEDLS + +SRFS+S++  S+P     IA+TW+ CARA +
Sbjct: 1014 YPYSSPIVLEEISFDTSVHKYEDLSARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATM 932

Query: 1014 SEYAQQSGGGSFCSKYGAWENCL 1020
             EYA++ GGG+F SKYGAWE  L
Sbjct: 1074 VEYAERHGGGTFSSKYGAWETVL 932

BLAST of Lag0014653 vs. ExPASy Swiss-Prot
Match: F4I169 (Probable mediator of RNA polymerase II transcription subunit 15b OS=Arabidopsis thaliana OX=3702 GN=MED15B PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.1e-30
Identity = 73/111 (65.77%), Postives = 87/111 (78.38%), Query Frame = 0

Query: 693 QSLAIGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALS 752
           QSL IGTPGISASPLL E +  DG   N L +  GKS+ TE P+ERLI+A+KS+SP+ALS
Sbjct: 150 QSLEIGTPGISASPLLPELTSPDGNIINPLTSTCGKSSATELPIERLIRAMKSISPQALS 209

Query: 753 ASVSGIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG 804
           ++V  I SVVSM+DRIAGS PG GSRA+ G DLVAMTKC LQ RNF++ DG
Sbjct: 210 SAVCDIRSVVSMVDRIAGSVPGKGSRASFGVDLVAMTKCHLQERNFMTQDG 260

HSP 2 Score: 64.3 bits (155), Expect = 9.1e-09
Identity = 68/202 (33.66%), Postives = 101/202 (50.00%), Query Frame = 0

Query: 269 ELKEMYQKILPKVHQFDSLP-QQPKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDK 328
           +L E+YQ++  K+ Q DSL  Q+ +S+Q  KLK  K +LE +L FL +SKSNI    KD 
Sbjct: 15  DLNEIYQRVAAKLQQEDSLSHQKQRSDQFEKLKRGKTVLEGMLRFLSLSKSNIKPDLKDS 74

Query: 329 IGHYEKQIVSFLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHS 388
           + + +  I++FL+    R    T+Q  QL  S +Q +QQ  +Q    QS ++Q   Q+ S
Sbjct: 75  MDYRKNNIMNFLNMQSLR---KTVQKLQLTKSEIQPMQQPLSQTVQDQSHDDQTTLQMQS 134

Query: 389 ANMQASVAPVQQNNMNNMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVA 448
            +MQ + + VQQ     +Q   + T   SA       P+ P   L S  GN ++ L    
Sbjct: 135 MSMQGAGSRVQQIRQGVLQSLEIGTPGISAS------PLLP--ELTSPDGNIINPLTSTC 194

Query: 449 A-GSLQQNPANSSQRANNSSLP 469
              S  + P     RA  S  P
Sbjct: 195 GKSSATELPIERLIRAMKSISP 205

BLAST of Lag0014653 vs. ExPASy TrEMBL
Match: A0A6J1JM75 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485927 PE=4 SV=1)

HSP 1 Score: 1288.9 bits (3334), Expect = 0.0e+00
Identity = 718/806 (89.08%), Postives = 745/806 (92.43%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 564  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 623

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 624  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 683

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 684  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 743

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 744  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 803

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 804  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 863

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 864  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 923

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 924  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 983

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 984  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 1043

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 1044 IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 1103

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 1104 GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1163

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1164 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1223

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1224 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1283

Query: 953  NYPNCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVV 1012
            NYPNCSPILLDKFP+EVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAVV
Sbjct: 1284 NYPNCSPILLDKFPIEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAVV 1343

Query: 1013 SEYAQQSGGGSFCSKYGAWENCLSGA 1023
            SEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1344 SEYAQQSGGGSFCSKYGAWENCLSAA 1367

BLAST of Lag0014653 vs. ExPASy TrEMBL
Match: A0A6J1JM75 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485927 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.5e-17
Identity = 161/512 (31.45%), Postives = 233/512 (45.51%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIY AAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYAAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQA 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSTNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKFQQ 277

Query: 399 SVAPVQ--QNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSLSSLQQVAAG 458
              P Q  Q+++   Q + +      + QQ++   MQP  S+L+  Q +S+    Q    
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMPPNQLQSSQQSV---MQPSLSNLQQNQQSSIQQPTQSMLQ 337

Query: 459 SLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQS--- 518
              Q      Q++   S+  Q    + Q ++S  Q    I Q  +        L+Q+   
Sbjct: 338 QPPQPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQQSNSSNMQQNSLIQNSVG 397

Query: 519 --QQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEI 578
             QQ L QQ +    Q     +Q  P   QQQ Q  QQ   Q S+     Q     Q  +
Sbjct: 398 DMQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQQLMNQQSSLSNLQQPQLGPQSNV 457

Query: 579 NDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSF--PISPPIFQATSPQVTQNSSP 638
           + L+ +Q    + G   ++ + Q   +  QQ K       P +P    +T  Q  Q  S 
Sbjct: 458 SGLQQQQMHGTQSGN-SNMQSNQHGVHMMQQNKVQMQQQPPQNPSNLLSTQGQQGQLQS- 517

Query: 639 QVDQQSLLSSIPKTGTPLQSASSPFVVPSPST 655
               Q L+S IP     +Q   S    P  +T
Sbjct: 518 ---SQQLMSQIPLQSAQVQQQVSLQQQPQSNT 520

HSP 2 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 720/801 (89.89%), Postives = 747/801 (93.26%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QAN GDWQEEIYQKIKAMKELYFFELKEMYQKILPK++QFDSLPQQPK
Sbjct: 569  STSLDSTAQTG-QANAGDWQEEIYQKIKAMKELYFFELKEMYQKILPKLNQFDSLPQQPK 628

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
             EQLNKLKAFK ILERLLAFLQV KSNIV+GFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 629  LEQLNKLKAFKSILERLLAFLQVPKSNIVLGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 688

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSLST 412
            PGQLPASHM SIQQSQ+QMT LQSPENQIN QLHSANMQ SVAPVQQNNMNNMQH+SLS+
Sbjct: 689  PGQLPASHMHSIQQSQSQMTSLQSPENQINSQLHSANMQCSVAPVQQNNMNNMQHNSLSS 748

Query: 413  FSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNG 472
            FSGSA QQNMT PMQPGS LESGQGNSLSSLQQVAAGSLQQNPAN+SQRAN+SSL SQNG
Sbjct: 749  FSGSAAQQNMTSPMQPGSGLESGQGNSLSSLQQVAAGSLQQNPANTSQRANSSSLSSQNG 808

Query: 473  VNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQM-QLKQHML----- 532
            VN LQ NIS LQSNPN+LQHQHLKQQDPQQL+QS Q LKQQ+QQR + QLKQ ML     
Sbjct: 809  VNPLQQNISALQSNPNMLQHQHLKQQDPQQLMQSPQQLKQQLQQRHIHQLKQQMLQQHQQ 868

Query: 533  -QQQPQLHQQQSQLHQQGKQQLST--QMPTHQMSHLNQI-EINDLKMRQGLAAKQGMFQH 592
             QQQPQ HQQQSQLHQQGKQQL T  QM  HQMSHLN I EINDLKMRQGLA K GMFQH
Sbjct: 869  QQQQPQSHQQQSQLHQQGKQQLPTQMQMQAHQMSHLNPINEINDLKMRQGLAVKPGMFQH 928

Query: 593  LPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTPLQS 652
            LPATQRSAY+H Q+KPGTSFPISPPIFQA SPQV QNSSPQVDQQSLLSSI KTGTPLQS
Sbjct: 929  LPATQRSAYSHPQLKPGTSFPISPPIFQAASPQVAQNSSPQVDQQSLLSSITKTGTPLQS 988

Query: 653  ASSPFVVPSPST-PAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLAIGTPG 712
            ASSPFVVPSPST P APSP+PGDSEKP+S V+S T+AGNTGQQTSV GTQAQSLAIGTPG
Sbjct: 989  ASSPFVVPSPSTSPVAPSPIPGDSEKPMSAVSSLTNAGNTGQQTSVSGTQAQSLAIGTPG 1048

Query: 713  ISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV 772
            ISASPLLAEFSG DG+Y NALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV
Sbjct: 1049 ISASPLLAEFSGTDGSYVNALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSV 1108

Query: 773  VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNV 832
            VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTKKM+R+TSAMPLNV
Sbjct: 1109 VSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGLNGTKKMRRYTSAMPLNV 1168

Query: 833  VSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVIS 892
            VSSAGSVNDV KPLTGAETSDLESTATSSVKRPRVEA+H+LLEEIREINQRLIDTVVVIS
Sbjct: 1169 VSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRVEANHVLLEEIREINQRLIDTVVVIS 1228

Query: 893  DEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYPNC 952
            DEVVDPSAL AAADGSEGTIVKCSFSAVALSP+LKSQY SAQMSPIQPLRLLVPTNYPNC
Sbjct: 1229 DEVVDPSALVAAADGSEGTIVKCSFSAVALSPNLKSQYASAQMSPIQPLRLLVPTNYPNC 1288

Query: 953  SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEYAQ 1012
            SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLG+IARTWD+CARAVVSEYAQ
Sbjct: 1289 SPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGEIARTWDVCARAVVSEYAQ 1348

Query: 1013 QSGGGSFCSKYGAWENCLSGA 1023
            QSGGGSFCSKYGAWENCLS A
Sbjct: 1349 QSGGGSFCSKYGAWENCLSAA 1368

BLAST of Lag0014653 vs. ExPASy TrEMBL
Match: A0A6J1DNN0 (mediator of RNA polymerase II transcription subunit 15a OS=Momordica charantia OX=3673 GN=LOC111021687 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.8e-12
Identity = 201/653 (30.78%), Postives = 277/653 (42.42%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTT-- 218
           METL R LPVSG++GLSELRKIAVRFEEKIYTAAT+QSDYLRKISLKMLTMETKSQ++  
Sbjct: 38  METLKRHLPVSGNEGLSELRKIAVRFEEKIYTAATSQSDYLRKISLKMLTMETKSQSSMG 97

Query: 219 PMVPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQK 278
             +PSNS+VP NKP+D+AS    +Q             I Q    +         +  Q+
Sbjct: 98  NPLPSNSMVPSNKPLDTASQSMPSQA------------INQSTVPLSS----NQPQSRQQ 157

Query: 279 ILPKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL--QVSKSNIVIGFKDKIGHYEKQ 338
           +L +  Q +++  Q  S   + +     +    +  +  Q S    V G    +G+   Q
Sbjct: 158 LLSQNIQ-NTIAPQSSSSLPSAVPPVTGLAPSSMPTMVGQSSSMQSVSGVSQNVGNTVGQ 217

Query: 339 IVS---FLHSNRP---RNPVSTLQPGQLPASHMQSIQ-QSQAQMTPLQSPENQINPQLHS 398
            V    F +S RP   R  VS  Q  Q  +      Q Q Q Q+   +  +  ++ QL  
Sbjct: 218 GVPSNVFPNSQRPIQGRQVVSQQQQQQSQSQQQYLYQHQLQQQLIKQKYQQGSMSHQLMQ 277

Query: 399 ANM-QASVAPVQQNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSL----- 458
            +M Q     +Q N + + Q S L T S   P       MQP  S+L+  Q + +     
Sbjct: 278 PHMSQQQQNLIQPNQLQSSQQSVLQTSSVMQPS-----VMQPSLSNLQQSQQSPIQQPTQ 337

Query: 459 SSLQQVAAGSLQQNPANSSQRA--------NNSSLPSQNGVNAL--QPNISPLQSNPNIL 518
           S LQQ +   L+Q   +    A          + LPSQ     +  QPN S +Q N  I 
Sbjct: 338 SMLQQPSQPVLRQQQQSQPHAAMHQQPTMPQQTMLPSQQQQQLISQQPNSSNMQQNSLIG 397

Query: 519 QHQHLKQQDPQQLLQSQQHLKQ----QMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQ 578
           Q   L     Q   QS+ H +Q     MQ    Q +Q ++ QQ  L    S L QQ +QQ
Sbjct: 398 QQSSLGDMQQQLPQQSRSHGQQSNLSNMQSPPSQQQQQLMTQQNNL----SNLQQQQQQQ 457

Query: 579 LSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSFPISP 638
           L    P   +S L Q           +   Q     L   Q      QQ  P TS  +S 
Sbjct: 458 LG---PQSNVSGLQQQIHGTQSGNSNMQTNQHSVHML--HQNKVQMQQQPPPNTSNLLS- 517

Query: 639 PIFQATSPQVTQNSSPQVD-QQSLLSSIPKTGT------PLQSASSPFVVPSPSTP---A 698
                     TQ    Q+   Q L+S IP   T      PLQ    P  V S        
Sbjct: 518 ----------TQGQQGQLQPSQQLMSQIPLQSTQVQQQVPLQQQQQPPNVMSHELQQRLQ 577

Query: 699 APSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSL--AIGTPGISASPLLAE---- 753
                PG   +P + +       ++  Q ++P T + SL     T   +A     E    
Sbjct: 578 VSGQAPGSLLQPQNVMDQQKQVYHS--QRALPETSSTSLDSTAQTGQANAGDWQEEIYQK 637

HSP 2 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 718/807 (88.97%), Postives = 745/807 (92.32%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 564  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 623

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 624  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 683

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 684  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 743

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 744  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 803

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 804  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 863

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 864  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 923

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 924  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 983

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 984  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 1043

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 1044 IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 1103

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 1104 GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1163

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1164 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1223

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1224 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1283

Query: 953  NYPNCSPILLDKFPVEV-SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAV 1012
            NYPNCSPILLDKFP+EV SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAV
Sbjct: 1284 NYPNCSPILLDKFPIEVSSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAV 1343

Query: 1013 VSEYAQQSGGGSFCSKYGAWENCLSGA 1023
            VSEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1344 VSEYAQQSGGGSFCSKYGAWENCLSAA 1368

BLAST of Lag0014653 vs. ExPASy TrEMBL
Match: A0A6J1JMY4 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485927 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.5e-17
Identity = 161/512 (31.45%), Postives = 233/512 (45.51%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIY AAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYAAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQA 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSTNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKFQQ 277

Query: 399 SVAPVQ--QNNMNNMQHSSLSTFSGSAPQQNMTIPMQPG-SSLESGQGNSLSSLQQVAAG 458
              P Q  Q+++   Q + +      + QQ++   MQP  S+L+  Q +S+    Q    
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMPPNQLQSSQQSV---MQPSLSNLQQNQQSSIQQPTQSMLQ 337

Query: 459 SLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQS--- 518
              Q      Q++   S+  Q    + Q ++S  Q    I Q  +        L+Q+   
Sbjct: 338 QPPQPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQQSNSSNMQQNSLIQNSVG 397

Query: 519 --QQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEI 578
             QQ L QQ +    Q     +Q  P   QQQ Q  QQ   Q S+     Q     Q  +
Sbjct: 398 DMQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQQLMNQQSSLSNLQQPQLGPQSNV 457

Query: 579 NDLKMRQGLAAKQGMFQHLPATQRSAYAHQQMKPGTSF--PISPPIFQATSPQVTQNSSP 638
           + L+ +Q    + G   ++ + Q   +  QQ K       P +P    +T  Q  Q  S 
Sbjct: 458 SGLQQQQMHGTQSGN-SNMQSNQHGVHMMQQNKVQMQQQPPQNPSNLLSTQGQQGQLQS- 517

Query: 639 QVDQQSLLSSIPKTGTPLQSASSPFVVPSPST 655
               Q L+S IP     +Q   S    P  +T
Sbjct: 518 ---SQQLMSQIPLQSAQVQQQVSLQQQPQSNT 520

HSP 2 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 718/807 (88.97%), Postives = 745/807 (92.32%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 459  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 518

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKIGHYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 519  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKIGHYEKQIVSFLNSNRPRNPVSTLQ 578

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ---------NNMN 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVAPVQQ         NNMN
Sbjct: 579  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVAPVQQNNMNNMNNMNNMN 638

Query: 413  NMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRAN 472
            NMQH+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPANSSQR N
Sbjct: 639  NMQHNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANSSQRVN 698

Query: 473  NSSLPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LK 532
            NSSL SQNGVN LQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LK
Sbjct: 699  NSSLASQNGVNVLQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLK 758

Query: 533  QHML------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAK 592
            Q ML      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIEINDLKMRQG+AAK
Sbjct: 759  QQMLQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEINDLKMRQGVAAK 818

Query: 593  QGMFQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKT 652
             GMFQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + 
Sbjct: 819  PGMFQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRI 878

Query: 653  GTPLQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLA 712
            GTPLQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLA
Sbjct: 879  GTPLQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLA 938

Query: 713  IGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVS 772
            IGTPGISASPLLAEFSG DGAYA ALPTVSGKS+ TEQPLERLIKAVKSMSPKAL+ASVS
Sbjct: 939  IGTPGISASPLLAEFSGTDGAYAIALPTVSGKSSDTEQPLERLIKAVKSMSPKALNASVS 998

Query: 773  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSA 832
            GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSA
Sbjct: 999  GIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSA 1058

Query: 833  MPLNVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDT 892
            MPLNVVSSAGSVNDV KPLTGAETSDLESTATSSVKRPR+EASH+LLEEIREINQRLIDT
Sbjct: 1059 MPLNVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRPRIEASHVLLEEIREINQRLIDT 1118

Query: 893  VVVISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 952
            VVVISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT
Sbjct: 1119 VVVISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPT 1178

Query: 953  NYPNCSPILLDKFPVEV-SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAV 1012
            NYPNCSPILLDKFP+EV SKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAV
Sbjct: 1179 NYPNCSPILLDKFPIEVSSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAV 1238

Query: 1013 VSEYAQQSGGGSFCSKYGAWENCLSGA 1023
            VSEYAQQSGGGSFCSKYGAWENCLS A
Sbjct: 1239 VSEYAQQSGGGSFCSKYGAWENCLSAA 1263

BLAST of Lag0014653 vs. ExPASy TrEMBL
Match: A0A6J1JHV7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111485927 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 6.3e-13
Identity = 177/578 (30.62%), Postives = 256/578 (44.29%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL R LPVSGH+GLSELRKIAVRFEEKIY AAT+QSDYLRKISLKMLTMETKSQTT +
Sbjct: 38  METLKRHLPVSGHEGLSELRKIAVRFEEKIYAAATSQSDYLRKISLKMLTMETKSQTTAL 97

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
            PSNS+VP NKP     LDST+Q+         Q ++  +  +M         +  Q++L
Sbjct: 98  -PSNSMVPTNKP-----LDSTSQS--------MQSQVLNQGPSMSGPMSSNQPQPRQQLL 157

Query: 279 PKVHQFDSLPQQPKSEQLNKLKAFKQILERLLAFL---QVSKSNIVIGFKDKIGHYEKQI 338
            +  Q +++  Q  S   + +     +    +A +     S  N+    +  +G+   Q 
Sbjct: 158 SQNIQ-NNIASQSSSSLPSSVPPVAGLASAPMANIVGQNPSMQNVSGVPQSSVGNAMGQG 217

Query: 339 VS---FLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQ- 398
           VS   F +S RP      +Q  Q+ +   Q  QQSQ+Q   L   +  +  Q+     Q 
Sbjct: 218 VSTNVFTNSQRP------IQGRQVVSQQQQQQQQSQSQQQQLFFQQQHLQQQIMKQKFQQ 277

Query: 399 --------ASVAPVQQNNM---NNMQHSSLS----TFSGSAPQQNMTIPMQPGSSLESGQ 458
                    S  P QQ N+   N +Q S  S    + S     Q  +I     S L+   
Sbjct: 278 GSMPHQLMQSHIPQQQTNLMPPNQLQSSQQSVMQPSLSNLQQNQQSSIQQPTQSMLQQPP 337

Query: 459 GNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNGVNALQPNISPLQSNPNILQHQ--H 518
              L   QQ    S+       SQ+A+ SS   Q  +N  Q N S +Q N +++Q+    
Sbjct: 338 QPVLRQQQQSQQHSVLHQQPTMSQQASLSSQQQQQLINQ-QSNSSNMQQN-SLIQNSVGD 397

Query: 519 LKQQDPQQ-LLQSQQHLKQQMQQRQMQLKQHMLQQQPQLHQQQSQLHQQGKQQLSTQMPT 578
           ++QQ PQQ     QQ     MQ    Q +Q   QQQPQ +    +L Q  + Q   Q P 
Sbjct: 398 MQQQLPQQSRSHGQQSNLSNMQTPPSQQQQQQQQQQPQSNTLSHELQQ--RLQAGGQAPG 457

Query: 579 HQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYAH------------QQMKPGTS 638
             +   N ++             +     L +T ++  A+            + MK    
Sbjct: 458 PLLQSQNVMDQQKQLYHPQRVLPETSSTSLDSTAQTGQANGGDWQEEIYQKIKSMKELYL 517

Query: 639 FPISPPIFQATSPQVTQ-NSSPQVDQQSLLSSIPKTGTPLQSASSPFVVPSPSTPAAPSP 696
           F +   ++Q   P+V Q +S PQ  +   L+ +      L+   +   VP  +       
Sbjct: 518 FELK-EMYQKILPKVHQFDSLPQQPKSEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKD 577

HSP 2 Score: 1283.1 bits (3319), Expect = 0.0e+00
Identity = 716/803 (89.17%), Postives = 744/803 (92.65%), Query Frame = 0

Query: 233  SASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPK 292
            S SLDSTAQTG QANGGDWQEEIYQKIK+MKELY FELKEMYQKILPKVHQFDSLPQQPK
Sbjct: 563  STSLDSTAQTG-QANGGDWQEEIYQKIKSMKELYLFELKEMYQKILPKVHQFDSLPQQPK 622

Query: 293  SEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 352
            SEQLNKL+AF+ ILERL+AFLQV K+NIVIGFKDKI HYEKQIVSFL+SNRPRNPVSTLQ
Sbjct: 623  SEQLNKLRAFRVILERLIAFLQVPKNNIVIGFKDKISHYEKQIVSFLNSNRPRNPVSTLQ 682

Query: 353  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQ------NNMNNMQ 412
             GQLPASHMQS+QQSQ+QMTPLQSPENQINPQLHSANMQ SVA VQQ      NNMNNMQ
Sbjct: 683  QGQLPASHMQSMQQSQSQMTPLQSPENQINPQLHSANMQGSVALVQQNNMNNMNNMNNMQ 742

Query: 413  HSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSS 472
            H+SL TFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQV A SLQQNPAN SQRANNSS
Sbjct: 743  HNSLPTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVGAVSLQQNPANGSQRANNSS 802

Query: 473  LPSQNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQ-LKQHM 532
            L SQNGVNALQPNIS LQSN NILQHQH+KQQDPQQLLQSQQ LKQQMQQR MQ LKQ M
Sbjct: 803  LASQNGVNALQPNISSLQSNTNILQHQHMKQQDPQQLLQSQQQLKQQMQQRHMQHLKQQM 862

Query: 533  L------QQQPQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGM 592
            L      QQQPQLHQQQSQL QQGKQQL TQM  HQMSHLNQIE+NDLKMRQG+AAK GM
Sbjct: 863  LQHQQQQQQQPQLHQQQSQLQQQGKQQLPTQMQAHQMSHLNQIEMNDLKMRQGVAAKPGM 922

Query: 593  FQHLPATQRSAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTP 652
            FQH P TQRSAY H QMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQ++ SS+ + GTP
Sbjct: 923  FQH-PGTQRSAYTHPQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQNMFSSMNRIGTP 982

Query: 653  LQSASSPFVVPSPSTPAAPSPMPGDSEKPISTVTSHTHAGNTGQQTSVPGTQAQSLAIGT 712
            LQSASSPFVVPSPSTP APSPMPGDSEKP S V+S  +AGNTGQQ +V G QA SLAIGT
Sbjct: 983  LQSASSPFVVPSPSTPLAPSPMPGDSEKPTSAVSSLPNAGNTGQQMNVSGAQAPSLAIGT 1042

Query: 713  PGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIG 772
            PGISASPLLAEFSG DGAYA ALPTVSGKS+VTEQPLERLIKAVKSMSP+AL+ASVSGIG
Sbjct: 1043 PGISASPLLAEFSGTDGAYAIALPTVSGKSSVTEQPLERLIKAVKSMSPRALNASVSGIG 1102

Query: 773  SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPL 832
            SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG NGTK+M+RHTSAMPL
Sbjct: 1103 SVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGSNGTKRMRRHTSAMPL 1162

Query: 833  NVVSSAGSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVV 892
            NVVSSAGSVNDV KPLTGAETSDLESTATSSVKR R+EASH+LLEEIREINQRLIDTVVV
Sbjct: 1163 NVVSSAGSVNDVFKPLTGAETSDLESTATSSVKRSRIEASHVLLEEIREINQRLIDTVVV 1222

Query: 893  ISDEVVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP 952
            ISDEVVDPSALAAAADGS+GTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP
Sbjct: 1223 ISDEVVDPSALAAAADGSDGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYP 1282

Query: 953  NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEY 1012
            NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWD+CARAVVSEY
Sbjct: 1283 NCSPILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDVCARAVVSEY 1342

Query: 1013 AQQSGGGSFCSKYGAWENCLSGA 1023
            AQQSGGGSFCSKYGAWENCLS A
Sbjct: 1343 AQQSGGGSFCSKYGAWENCLSAA 1363

BLAST of Lag0014653 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 735.7 bits (1898), Expect = 5.0e-212
Identity = 464/799 (58.07%), Postives = 575/799 (71.96%), Query Frame = 0

Query: 231  MDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQ 290
            M S+SLDSTAQT + ANGGDWQEE+YQKIK+MKE Y  +L E+YQ++  K+ Q DS+PQQ
Sbjct: 574  MPSSSLDSTAQT-ESANGGDWQEEVYQKIKSMKETYLPDLNEIYQRVAAKLQQ-DSMPQQ 633

Query: 291  PKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVST 350
             +S+QL KL+ FK +LER++ FL VSKSNI+   KDK+ +YEKQI+ FL+ +RPR PV  
Sbjct: 634  QRSDQLEKLRQFKTMLERMIQFLSVSKSNIMPALKDKVAYYEKQIIGFLNMHRPRKPV-- 693

Query: 351  LQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSL 410
             Q GQLP S MQ +QQ Q+Q    QS +NQ NPQ+ S +MQ +    QQ++M NMQ + L
Sbjct: 694  -QQGQLPQSQMQPMQQPQSQTVQDQSHDNQTNPQMQSMSMQGAGPRAQQSSMTNMQSNVL 753

Query: 411  STFSG-SAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPS 470
            S+  G SAPQQN+   + P SSLESGQGN+L++ QQVA GS+QQ   N+SQ  NNSS  +
Sbjct: 754  SSRPGVSAPQQNIPSSI-PASSLESGQGNTLNNGQQVAMGSMQQ---NTSQLVNNSSASA 813

Query: 471  QNGVNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQLKQHMLQQQ 530
            Q+G++ LQ N++  Q + ++LQHQHLKQQ  QQ+   QQ  ++QMQQ+Q+Q +Q   QQQ
Sbjct: 814  QSGLSTLQSNVNQPQLSSSLLQHQHLKQQQDQQMQLKQQFQQRQMQQQQLQARQQQQQQQ 873

Query: 531  PQLHQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMF-QHLPATQR 590
             Q  QQ +QL Q                     ++NDL  RQG+   +GMF QH    QR
Sbjct: 874  LQARQQAAQLQQMN-------------------DMNDLTSRQGMNVSRGMFQQHSMQGQR 933

Query: 591  SAYAHQQMKPGTSFPISPPIFQATSPQVTQNSSPQVDQQSLLSSIPKTGTPLQSASSPFV 650
            + Y  QQ+KPG     SP + Q  SPQ++Q+ SPQVDQ+   +++ K GTPLQ A+SPFV
Sbjct: 934  ANYPLQQLKPGA--VSSPQLLQGASPQMSQHLSPQVDQK---NTVNKMGTPLQPANSPFV 993

Query: 651  VPSP-STPAAPSPMPGDSEKPISTVTSHTHAGNTGQQ--TSVPGTQAQSLAIGTPGISAS 710
            VPSP STP APSPM  DSEKP S+  S    GN  +Q  T + G   QSLAIGTPGISAS
Sbjct: 994  VPSPSSTPLAPSPMQVDSEKPGSSSLS---MGNIARQQATGMQGV-VQSLAIGTPGISAS 1053

Query: 711  PLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSVVSMI 770
            PLL EF+  DG   N+    SGK + TE P+ERLI+AVKS+SP+ALS++VS IGSVVSM+
Sbjct: 1054 PLLQEFTSPDGNILNSSTITSGKPSATELPIERLIRAVKSISPQALSSAVSDIGSVVSMV 1113

Query: 771  DRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNVVSSA 830
            DRIAGSAPGNGSRA+VGEDLVAMTKCRLQARNF++ +G   TKKMKRHT+AMPL+V S  
Sbjct: 1114 DRIAGSAPGNGSRASVGEDLVAMTKCRLQARNFMTQEGMMATKKMKRHTTAMPLSVASLG 1173

Query: 831  GSVNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVISD--E 890
            GSV D  K   G+ETSDLESTATS  K+ R E  H LLEEI+EINQRLIDTVV ISD  +
Sbjct: 1174 GSVGDNYKQFAGSETSDLESTATSDGKKARTETEHALLEEIKEINQRLIDTVVEISDDED 1233

Query: 891  VVDPSALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYPNCSP 950
              DPS +A ++ G EGT V+ SF AV+LSP+LK+  +S QMSPIQPLRLLVP +YPN SP
Sbjct: 1234 AADPSEVAISSIGCEGTTVRFSFIAVSLSPALKAHLSSTQMSPIQPLRLLVPCSYPNGSP 1293

Query: 951  ILLDKFPVEVSKEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEYAQQS 1010
             LLDK PVE SKE EDLS KA +RF+I LR+LSQPMSL DIA+TWD CARAV+ EYAQQ 
Sbjct: 1294 SLLDKLPVETSKENEDLSSKAMARFNILLRSLSQPMSLKDIAKTWDACARAVICEYAQQF 1335

Query: 1011 GGGSFCSKYGAWENCLSGA 1023
            GGG+F SKYG WE  ++ +
Sbjct: 1354 GGGTFSSKYGTWEKYVAAS 1335

HSP 2 Score: 63.2 bits (152), Expect = 1.4e-09
Identity = 125/430 (29.07%), Postives = 182/430 (42.33%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQTTPM 218
           METL + LP SG +G++ELR+IA RFEEKI++ A NQ+DYLRKIS+KMLTMETKSQ    
Sbjct: 39  METLKKHLPFSGPEGINELRRIAARFEEKIFSGALNQTDYLRKISMKMLTMETKSQNA-- 98

Query: 219 VPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKIL 278
             S++ +P     +  S+DS      Q   G       Q  + +        + M     
Sbjct: 99  AGSSAAIPAAN--NGTSIDSIPTNQGQLLPGSLSTNQSQAPQPLLS------QTMQNNTA 158

Query: 279 PKVHQFDSLP-QQPKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVS 338
             +    +LP   P    +        + +        +  N+    +D  G +      
Sbjct: 159 SGMTGSTALPSSMPPVSSITNNNTTSVVNQ------NANMQNVAGMLQDSSGQHGLSSNM 218

Query: 339 FLHSNRP---RNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASV 398
           F    R    R    + Q  Q P  + Q +QQ   +           N  L S   Q   
Sbjct: 219 FSGPQRQMLGRPHAMSSQQQQQPYLYQQQLQQQLLKQNFQSGNVPNPNSLLPSHIQQQQQ 278

Query: 399 APVQQNNMNNMQHSSLSTFS------GSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAA 458
             +Q N +++ Q   + T +       SAP Q +    Q    L S Q  + S L+Q  +
Sbjct: 279 NVLQPNQLHSSQQPGVPTSATQPSTVNSAPLQGLHTNQQSSPQL-SSQQTTQSMLRQHQS 338

Query: 459 GSLQQNPAN---SSQRANNSSLPSQNGVNALQPNISPLQSNP-NILQHQHLKQQDPQQLL 518
             L+Q+P +   S      SSLP Q        +ISPLQ  P  +++ Q       QQ  
Sbjct: 339 SMLRQHPQSQQASGIHQQQSSLPQQ--------SISPLQQQPTQLMRQQAANSSGIQQKQ 398

Query: 519 QSQQH----LKQQMQQR-------QMQLKQHMLQQQP-QLHQQQSQLHQQGKQQLSTQMP 563
              QH    ++QQ QQR        M ++Q   QQQP Q  QQQ +     +QQL +Q  
Sbjct: 399 MMGQHVVGDMQQQHQQRLLNQQNNVMNIQQQQSQQQPLQQPQQQQKQQPPAQQQLMSQQN 443

BLAST of Lag0014653 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 289.7 bits (740), Expect = 9.5e-78
Identity = 278/793 (35.06%), Postives = 395/793 (49.81%), Query Frame = 0

Query: 234  ASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPKS 293
            AS DST +T    N G+WQEE YQKIK +KE+    L  M+Q++  K+ + +SLP QP  
Sbjct: 214  ASQDSTGKT-VNVNAGNWQEETYQKIKKLKEMCLPVLSLMHQRVAEKLRETESLPPQPMQ 273

Query: 294  EQ-LNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 353
             Q + KLKA K  +E L+ FL V +S++    +DK   YE  I+ F  S       +  Q
Sbjct: 274  AQWIEKLKAGKLSMEHLMFFLNVHRSSVSEKHRDKFSQYEYHILKFTKSQTMVLRPTQQQ 333

Query: 354  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSLST 413
             GQ P S  Q+  Q+       QSP+  ++  L+    ++ + P  QN     + SSL  
Sbjct: 334  QGQFPPS--QTAMQT-------QSPQVHVSQSLYKEQRRSRLMPSSQN-----EASSLLQ 393

Query: 414  FSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNG 473
                   ++  I M       +  GN       V   S++QNP                 
Sbjct: 394  IRPKLDPRDENIIM-------ASSGN-------VMLPSVKQNP----------------- 453

Query: 474  VNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQLKQHMLQQQPQL 533
              A+  NIS +QS                  LQ Q    ++   RQMQ      QQQP  
Sbjct: 454  -RAVNTNISSVQS------------------LQKQ----KRFHHRQMQ------QQQP-- 513

Query: 534  HQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYA 593
                    QQG  Q       HQM      E+ND++MR+ +  K  + +   ++ +    
Sbjct: 514  --------QQGNHQ-------HQM---QTNEMNDVRMRERVNIKARLLEQQVSSSQRQVP 573

Query: 594  HQQMKPGTSFPISPPIFQATSPQVTQNSSPQ-VDQQSLLSSIPKTGTPLQSASSPFVVPS 653
             Q+                +S Q+  +SSPQ VDQ  L ++I KTGTPL S+ S FV P 
Sbjct: 574  KQES-------------NVSSSQIQNHSSPQLVDQHILPATINKTGTPLNSSGSAFVAP- 633

Query: 654  PSTPAAPSPMPGDSEKPIST---VTSHTHAGNTGQQTSVPGTQAQSLAIGTPGISASPLL 713
                 APSP+PGDSE PIS    V+      +T   +S  GTQ             +PLL
Sbjct: 634  -----APSPVPGDSEMPISVESPVSGVDEINSTLDSSSKLGTQ------------ETPLL 693

Query: 714  AEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSVVSMIDRI 773
                            V     +TE+P++RLIKA ++ SPK+L+ SVS I SV+SM+D I
Sbjct: 694  ---------------FVPPPEPITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMI 753

Query: 774  AGSAPGN-GSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNVVSSAGS 833
             GS P + GSRA +GEDL   T      RNF +H+  N +K+MKR  + +P ++ S    
Sbjct: 754  GGSFPSSGGSRAGLGEDLSERT------RNFTTHEETNLSKRMKRSINIVPPDMSSQI-- 813

Query: 834  VNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVISDEVVDP 893
              D  + L+  E S++ ST +S +K   +   + LL+EI+E N RL++TVV I DE    
Sbjct: 814  --DSYEQLSSLE-SEVVSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDE---- 842

Query: 894  SALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQMSPIQPLRLLVPTNYPNCSPILLD 953
                     S GTIV C+++ VALS + K  Y S +++ IQPLRLL P +YP  SPI+L+
Sbjct: 874  --------DSLGTIVTCTYAPVALSATFKDHYKSGKIAQIQPLRLLFPMDYPYSSPIVLE 842

Query: 954  KFPVEVS-KEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVVSEYAQQSGGG 1013
            +   + S  +YEDLS + +SRFS+S++  S+P     IA+TW+ CARA + EYA++ GGG
Sbjct: 934  EISFDTSVHKYEDLSARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGG 842

Query: 1014 SFCSKYGAWENCL 1020
            +F SKYGAWE  L
Sbjct: 994  TFSSKYGAWETVL 842

BLAST of Lag0014653 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 281.6 bits (719), Expect = 2.6e-75
Identity = 278/803 (34.62%), Postives = 395/803 (49.19%), Query Frame = 0

Query: 234  ASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYFFELKEMYQKILPKVHQFDSLPQQPKS 293
            AS DST +T    N G+WQEE YQKIK +KE+    L  M+Q++  K+ + +SLP QP  
Sbjct: 294  ASQDSTGKT-VNVNAGNWQEETYQKIKKLKEMCLPVLSLMHQRVAEKLRETESLPPQPMQ 353

Query: 294  EQ-LNKLKAFKQILERLLAFLQVSKSNIVIGFKDKIGHYEKQIVSFLHSNRPRNPVSTLQ 353
             Q + KLKA K  +E L+ FL V +S++    +DK   YE  I+ F  S       +  Q
Sbjct: 354  AQWIEKLKAGKLSMEHLMFFLNVHRSSVSEKHRDKFSQYEYHILKFTKSQTMVLRPTQQQ 413

Query: 354  PGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHSANMQASVAPVQQNNMNNMQHSSLST 413
             GQ P S  Q+  Q+       QSP+  ++  L+    ++ + P  QN     + SSL  
Sbjct: 414  QGQFPPS--QTAMQT-------QSPQVHVSQSLYKEQRRSRLMPSSQN-----EASSLLQ 473

Query: 414  FSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVAAGSLQQNPANSSQRANNSSLPSQNG 473
                   ++  I M       +  GN       V   S++QNP                 
Sbjct: 474  IRPKLDPRDENIIM-------ASSGN-------VMLPSVKQNP----------------- 533

Query: 474  VNALQPNISPLQSNPNILQHQHLKQQDPQQLLQSQQHLKQQMQQRQMQLKQHMLQQQPQL 533
              A+  NIS +QS                  LQ Q    ++   RQMQ      QQQP  
Sbjct: 534  -RAVNTNISSVQS------------------LQKQ----KRFHHRQMQ------QQQP-- 593

Query: 534  HQQQSQLHQQGKQQLSTQMPTHQMSHLNQIEINDLKMRQGLAAKQGMFQHLPATQRSAYA 593
                    QQG  Q       HQM      E+ND++MR+ +  K  + +   ++ +    
Sbjct: 594  --------QQGNHQ-------HQM---QTNEMNDVRMRERVNIKARLLEQQVSSSQRQVP 653

Query: 594  HQQMKPGTSFPISPPIFQATSPQVTQNSSPQ-VDQQSLLSSIPKTGTPLQSASSPFVVPS 653
             Q+                +S Q+  +SSPQ VDQ  L ++I KTGTPL S+ S FV P 
Sbjct: 654  KQES-------------NVSSSQIQNHSSPQLVDQHILPATINKTGTPLNSSGSAFVAP- 713

Query: 654  PSTPAAPSPMPGDSEKPIST---VTSHTHAGNTGQQTSVPGTQAQSLAIGTPGISASPLL 713
                 APSP+PGDSE PIS    V+      +T   +S  GTQ             +PLL
Sbjct: 714  -----APSPVPGDSEMPISVESPVSGVDEINSTLDSSSKLGTQ------------ETPLL 773

Query: 714  AEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALSASVSGIGSVVSMIDRI 773
                            V     +TE+P++RLIKA ++ SPK+L+ SVS I SV+SM+D I
Sbjct: 774  ---------------FVPPPEPITERPIDRLIKAFQAASPKSLAESVSEISSVISMVDMI 833

Query: 774  AGSAPGN-GSRAAVGEDLVAMTKCRLQARNFVSHDGPNGTKKMKRHTSAMPLNVVSSAGS 833
             GS P + GSRA +GEDL   T      RNF +H+  N +K+MKR  + +P ++ S    
Sbjct: 834  GGSFPSSGGSRAGLGEDLSERT------RNFTTHEETNLSKRMKRSINIVPPDMSSQI-- 893

Query: 834  VNDVLKPLTGAETSDLESTATSSVKRPRVEASHILLEEIREINQRLIDTVVVISDEVVDP 893
              D  + L+  E S++ ST +S +K   +   + LL+EI+E N RL++TVV I DE    
Sbjct: 894  --DSYEQLSSLE-SEVVSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDE---- 932

Query: 894  SALAAAADGSEGTIVKCSFSAVALSPSLKSQYTSAQM----------SPIQPLRLLVPTN 953
                     S GTIV C+++ VALS + K  Y S ++          + IQPLRLL P +
Sbjct: 954  --------DSLGTIVTCTYAPVALSATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMD 932

Query: 954  YPNCSPILLDKFPVEVS-KEYEDLSIKAKSRFSISLRNLSQPMSLGDIARTWDICARAVV 1013
            YP  SPI+L++   + S  +YEDLS + +SRFS+S++  S+P     IA+TW+ CARA +
Sbjct: 1014 YPYSSPIVLEEISFDTSVHKYEDLSARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATM 932

Query: 1014 SEYAQQSGGGSFCSKYGAWENCL 1020
             EYA++ GGG+F SKYGAWE  L
Sbjct: 1074 VEYAERHGGGTFSSKYGAWETVL 932

BLAST of Lag0014653 vs. TAIR 10
Match: AT1G15770.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 137.1 bits (344), Expect = 7.8e-32
Identity = 73/111 (65.77%), Postives = 87/111 (78.38%), Query Frame = 0

Query: 693 QSLAIGTPGISASPLLAEFSGADGAYANALPTVSGKSNVTEQPLERLIKAVKSMSPKALS 752
           QSL IGTPGISASPLL E +  DG   N L +  GKS+ TE P+ERLI+A+KS+SP+ALS
Sbjct: 150 QSLEIGTPGISASPLLPELTSPDGNIINPLTSTCGKSSATELPIERLIRAMKSISPQALS 209

Query: 753 ASVSGIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARNFVSHDG 804
           ++V  I SVVSM+DRIAGS PG GSRA+ G DLVAMTKC LQ RNF++ DG
Sbjct: 210 SAVCDIRSVVSMVDRIAGSVPGKGSRASFGVDLVAMTKCHLQERNFMTQDG 260

HSP 2 Score: 64.3 bits (155), Expect = 6.5e-10
Identity = 68/202 (33.66%), Postives = 101/202 (50.00%), Query Frame = 0

Query: 269 ELKEMYQKILPKVHQFDSLP-QQPKSEQLNKLKAFKQILERLLAFLQVSKSNIVIGFKDK 328
           +L E+YQ++  K+ Q DSL  Q+ +S+Q  KLK  K +LE +L FL +SKSNI    KD 
Sbjct: 15  DLNEIYQRVAAKLQQEDSLSHQKQRSDQFEKLKRGKTVLEGMLRFLSLSKSNIKPDLKDS 74

Query: 329 IGHYEKQIVSFLHSNRPRNPVSTLQPGQLPASHMQSIQQSQAQMTPLQSPENQINPQLHS 388
           + + +  I++FL+    R    T+Q  QL  S +Q +QQ  +Q    QS ++Q   Q+ S
Sbjct: 75  MDYRKNNIMNFLNMQSLR---KTVQKLQLTKSEIQPMQQPLSQTVQDQSHDDQTTLQMQS 134

Query: 389 ANMQASVAPVQQNNMNNMQHSSLSTFSGSAPQQNMTIPMQPGSSLESGQGNSLSSLQQVA 448
            +MQ + + VQQ     +Q   + T   SA       P+ P   L S  GN ++ L    
Sbjct: 135 MSMQGAGSRVQQIRQGVLQSLEIGTPGISAS------PLLP--ELTSPDGNIINPLTSTC 194

Query: 449 A-GSLQQNPANSSQRANNSSLP 469
              S  + P     RA  S  P
Sbjct: 195 GKSSATELPIERLIRAMKSISP 205

BLAST of Lag0014653 vs. TAIR 10
Match: AT1G15790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 170 Blast hits to 94 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 170; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 72.0 bits (175), Expect = 3.1e-12
Identity = 52/132 (39.39%), Postives = 72/132 (54.55%), Query Frame = 0

Query: 159 METLSRVLPVSGHDGLSELRKIAVRFEEKIYTAATNQSDYLRKISLKMLTMETKSQ---- 218
           MET  + LP    +G +ELRKIAVRFEEK++  A+NQ++YLR+I +KML METKSQ    
Sbjct: 24  METQLKQLPFIRPEGTNELRKIAVRFEEKLFNNASNQTEYLRQICMKMLNMETKSQNAAG 83

Query: 219 -------TTPMVPSNSLVPGNKPMDSASLDSTAQTGQQANGGDWQEEIYQKIKAMKELYF 278
                  T P+VP  S VP N+P                N GDW+    Q+ +  ++   
Sbjct: 84  SSSADDNTPPLVPEPS-VPNNEP--------------AVNTGDWRT---QQPQDSRQKNI 137

Query: 279 FELKEMYQKILP 280
             L +  +KI+P
Sbjct: 144 NALLDTLKKIVP 137