Lag0014649 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0014649
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-alkenal reductase (NADP(+)-dependent)-like
Location	chr12: 3052765 .. 3053095 (+)
RNA-Seq Expression	Lag0014649
Synteny	Lag0014649

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCGACGCAGTGCTCAAAAACATGAGGCCAAAAGGGCGCATAGCAGCGTGTGGGATGATATCGCAGTACAATCTGGAGCAGCATGAAGGCGTATACAACTTGGTGAATGTAGTACAAAAGCGGATTCGAATGGAAGGCTTCGAGGCCCCCCGATTTCTTCCATCTCTATCCCAAGTTTCTGGCCGAGGTTCTGCCGTTCATCAGAAATGGGGAGATTGTTTATGTCGAGGACGTGGCAGAGGGCCTTGATGCTGCCCCAGCTGCCCTCGTTGGCCTCTTCTCTGGACGAAACCTTGGCAAACAACTTGTTGCTGTTGCTCAACCTTGA

mRNA sequence

ATGCTCGACGCAGTGCTCAAAAACATGAGGCCAAAAGGGCGCATAGCAGCGTGTGGGATGATATCGCAGTACAATCTGGAGCAGCATGAAGGCGTATACAACTTGGTGAATGTAGTACAAAAGCGGATTCGAATGGAAGGCTTCGAGGCCCCCCGATTTCTTCCATCTCTATCCCAAGTTTCTGGCCGAGAGGGCCTTGATGCTGCCCCAGCTGCCCTCGTTGGCCTCTTCTCTGGACGAAACCTTGGCAAACAACTTGTTGCTGTTGCTCAACCTTGA

Coding sequence (CDS)

ATGCTCGACGCAGTGCTCAAAAACATGAGGCCAAAAGGGCGCATAGCAGCGTGTGGGATGATATCGCAGTACAATCTGGAGCAGCATGAAGGCGTATACAACTTGGTGAATGTAGTACAAAAGCGGATTCGAATGGAAGGCTTCGAGGCCCCCCGATTTCTTCCATCTCTATCCCAAGTTTCTGGCCGAGAGGGCCTTGATGCTGCCCCAGCTGCCCTCGTTGGCCTCTTCTCTGGACGAAACCTTGGCAAACAACTTGTTGCTGTTGCTCAACCTTGA

Protein sequence

MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRFLPSLSQVSGREGLDAAPAALVGLFSGRNLGKQLVAVAQP

Homology

[Close]

BLAST of Lag0014649 vs. NCBI nr
Match: XP_022154379.1 (2-alkenal reductase (NADP(+)-dependent)-like isoform X2 [Momordica charantia] >XP_022154380.1 2-alkenal reductase (NADP(+)-dependent)-like isoform X2 [Momordica charantia] >XP_022154381.1 2-alkenal reductase (NADP(+)-dependent)-like isoform X2 [Momordica charantia] >XP_022154382.1 2-alkenal reductase (NADP(+)-dependent)-like isoform X2 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-22
Identity = 66/107 (61.68%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRF------- 60
           MLDAVL+NMR +GRIAACGM+SQYNLEQ EGV+NL+ VV +RIR+EGFEAP F       
Sbjct: 238 MLDAVLQNMRQRGRIAACGMMSQYNLEQKEGVHNLLYVVTQRIRIEGFEAPDFFHQYPKF 297

Query: 61  ----LPSLSQ------VSGREGLDAAPAALVGLFSGRNLGKQLVAVA 91
               LP + +          EGLDAAPAALVGLF+G+N GKQLVA+A
Sbjct: 298 LDAVLPYIREGKIAYVEDVAEGLDAAPAALVGLFTGQNTGKQLVALA 344

[Close]

BLAST of Lag0014649 vs. NCBI nr
Match: XP_022154378.1 (2-alkenal reductase (NADP(+)-dependent)-like isoform X1 [Momordica charantia])

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-22
Identity = 66/107 (61.68%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRF------- 60
           MLDAVL+NMR +GRIAACGM+SQYNLEQ EGV+NL+ VV +RIR+EGFEAP F       
Sbjct: 249 MLDAVLQNMRQRGRIAACGMMSQYNLEQKEGVHNLLYVVTQRIRIEGFEAPDFFHQYPKF 308

Query: 61  ----LPSLSQ------VSGREGLDAAPAALVGLFSGRNLGKQLVAVA 91
               LP + +          EGLDAAPAALVGLF+G+N GKQLVA+A
Sbjct: 309 LDAVLPYIREGKIAYVEDVAEGLDAAPAALVGLFTGQNTGKQLVALA 355

[Close]

BLAST of Lag0014649 vs. NCBI nr
Match: OVA10647.1 (Alcohol dehydrogenase superfamily [Macleaya cordata])

HSP 1 Score: 115.9 bits (289), Expect = 1.8e-22
Identity = 64/108 (59.26%), Postives = 77/108 (71.30%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEA-------PRF 60
           MLDAVL NMRP GRIA CGMISQYNLEQHEGV+NL  ++ KRIRM+GF A       P+F
Sbjct: 630 MLDAVLSNMRPHGRIAVCGMISQYNLEQHEGVHNLFLIITKRIRMQGFIAGDQYHLYPKF 689

Query: 61  LPSLSQ--VSGR--------EGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
           +  + Q    G+        EGL+ APAALVGLF+GRN+GKQ+V VA+
Sbjct: 690 IEMMKQYITEGKIVYVEDIVEGLENAPAALVGLFTGRNVGKQVVKVAR 737

[Close]

BLAST of Lag0014649 vs. NCBI nr
Match: XP_031258512.1 (2-alkenal reductase (NADP(+)-dependent)-like [Pistacia vera])

HSP 1 Score: 112.1 bits (279), Expect = 2.6e-21
Identity = 62/108 (57.41%), Postives = 75/108 (69.44%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRFLPSLSQV 60
           MLDAVL NMR +GRIAACGMISQYNL Q EGV+NL+N+V KRIRMEG  A  + P  ++ 
Sbjct: 241 MLDAVLLNMRLRGRIAACGMISQYNLHQPEGVHNLMNIVGKRIRMEGLMAFDYFPQYAKF 300

Query: 61  SG-----------------REGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
                               EG+++APAALVGLFSGRN+GKQ+VAVA+
Sbjct: 301 LDVVLPFVREKKVVYVKDIAEGIESAPAALVGLFSGRNVGKQVVAVAK 348

[Close]

BLAST of Lag0014649 vs. NCBI nr
Match: XP_031258502.1 (2-alkenal reductase (NADP(+)-dependent)-like [Pistacia vera])

HSP 1 Score: 111.7 bits (278), Expect = 3.4e-21
Identity = 62/108 (57.41%), Postives = 74/108 (68.52%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRFLPSLSQV 60
           MLDAVL NMR +GRIAACGMISQYNL Q EGV+NL+N+V KRIRMEG  A  + P   + 
Sbjct: 241 MLDAVLLNMRLRGRIAACGMISQYNLHQPEGVHNLMNIVGKRIRMEGLMAFDYFPQYEKF 300

Query: 61  SG-----------------REGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
                               EG+++APAALVGLFSGRN+GKQ+VAVA+
Sbjct: 301 LDAVLPFVREKKVVYVEDIAEGIESAPAALVGLFSGRNVGKQVVAVAK 348

[Close]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot
Match: Q39173 (NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 4.6e-21
Identity = 56/108 (51.85%), Postives = 72/108 (66.67%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGF-------EAPRF 60
           MLDAVL NM P GRIA CGMISQYNLE  EGV+NL N++ KRIR++GF       + P+F
Sbjct: 235 MLDAVLLNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVADFYDKYPKF 294

Query: 61  ----LPSLSQ------VSGREGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
               LP + +          +GL+ AP ALVGLF G+N+GKQ+V +A+
Sbjct: 295 LELVLPRIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIAR 342

[Close]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot
Match: Q9SLN8 (2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.8e-20
Identity = 58/108 (53.70%), Postives = 72/108 (66.67%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEA-------PRF 60
           MLDAVL NM+  GRIA CGMISQYNLEQ EGV+NL  ++ KRIRMEGF         P++
Sbjct: 235 MLDAVLVNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKY 294

Query: 61  LPS-LSQVSG---------REGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
           L   + Q+             GL++AP ALVGLFSGRN+GKQ+V V++
Sbjct: 295 LEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSR 342

[Close]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot
Match: Q39172 (NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=3702 GN=AER PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 5.1e-20
Identity = 55/108 (50.93%), Postives = 68/108 (62.96%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRFLPSLSQV 60
           MLDAVL NM   GRIA CGMISQYNLE  EGV+NL N++ KRIR++GF    F    S+ 
Sbjct: 237 MLDAVLVNMNMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVSDFYDKYSKF 296

Query: 61  ---------SGR--------EGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
                     G+        +GL+ AP ALVGLF G+N+GKQ+V VA+
Sbjct: 297 LEFVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVAR 344

[Close]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot
Match: Q6WAU0 ((+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.7e-18
Identity = 50/108 (46.30%), Postives = 74/108 (68.52%), Query Frame = 0


Query: 1   MLDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEA-------PRF 60
           ML+AV+ NMR  GRIA CGM+SQY+L+Q EGV+NL+ ++ K+IRM+GF         P+F
Sbjct: 234 MLEAVINNMRVHGRIAVCGMVSQYSLKQPEGVHNLLKLIPKQIRMQGFVVVDYYHLYPKF 293

Query: 61  ----LPSLSQ------VSGREGLDAAPAALVGLFSGRNLGKQLVAVAQ 92
               LP + +          EGL++AP+AL+G++ GRN+G Q+VAV++
Sbjct: 294 LEMVLPRIKEGKVTYVEDISEGLESAPSALLGVYVGRNVGNQVVAVSR 341

[Close]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot
Match: Q9C0Y6 (Zinc-type alcohol dehydrogenase-like protein PB24D3.08c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAPB24D3.08c PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.8e-09
Identity = 38/107 (35.51%), Postives = 55/107 (51.40%), Query Frame = 0


Query: 2   LDAVLKNMRPKGRIAACGMISQYNLEQHEGVYNLVNVVQKRIRMEGFEAPRFLPSLSQ-- 61
           +DAVL+NM  +GRI  CG ISQYN      V NL  V+ K + ++GF     LP   +  
Sbjct: 241 MDAVLENMNLQGRIIFCGAISQYNNPNPYRVKNLGMVLVKSLTIQGFIVANILPQYQEQY 300

Query: 62  --------VSGR--------EGLDAAPAALVGLFSGRNLGKQLVAVA 91
                     G+        +GL++AP A +G+  G+N GK +V +A
Sbjct: 301 FEEMPKLIAEGKIKYKCDVYDGLESAPEAFIGMLQGKNSGKTIVKIA 347

The following BLAST results are available for this feature:

BLAST of Lag0014649 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154379.1	1.1e-22	61.68	2-alkenal reductase (NADP(+)-dependent)-like isoform X2 [Momordica charantia] >X...	[more]
XP_022154378.1	1.1e-22	61.68	2-alkenal reductase (NADP(+)-dependent)-like isoform X1 [Momordica charantia]	[more]
OVA10647.1	1.8e-22	59.26	Alcohol dehydrogenase superfamily [Macleaya cordata]	[more]
XP_031258512.1	2.6e-21	57.41	2-alkenal reductase (NADP(+)-dependent)-like [Pistacia vera]	[more]
XP_031258502.1	3.4e-21	57.41	2-alkenal reductase (NADP(+)-dependent)-like [Pistacia vera]	[more]

BLAST of Lag0014649 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39173	4.6e-21	51.85	NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SLN8	1.8e-20	53.70	2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1...	[more]
Q39172	5.1e-20	50.93	NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=37...	[more]
Q6WAU0	3.7e-18	46.30	(+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1	[more]
Q9C0Y6	1.8e-09	35.51	Zinc-type alcohol dehydrogenase-like protein PB24D3.08c OS=Schizosaccharomyces p...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.180.10		coord: 61..87 e-value: 7.7E-19 score: 69.8
None	No IPR available	GENE3D	3.40.50.720		coord: 1..60 e-value: 7.7E-19 score: 69.8
None	No IPR available	PANTHER	PTHR43205:SF53	DEHYDROGENASE, PUTATIVE-RELATED	coord: 1..53 coord: 64..91
IPR045010	Medium-chain dehydrogenase/reductase	PANTHER	PTHR43205	PROSTAGLANDIN REDUCTASE	coord: 1..53 coord: 64..91
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 1..48

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0014649.1	Lag0014649.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006979	response to oxidative stress
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)+] activity
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor