Homology
BLAST of Lag0014578 vs. NCBI nr
Match:
XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])
HSP 1 Score: 760.0 bits (1961), Expect = 1.1e-215
Identity = 361/406 (88.92%), Postives = 385/406 (94.83%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKSSSL 407
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406
BLAST of Lag0014578 vs. NCBI nr
Match:
XP_022952781.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita moschata])
HSP 1 Score: 758.1 bits (1956), Expect = 4.0e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. NCBI nr
Match:
XP_022952784.1 (triacylglycerol lipase 1 isoform X3 [Cucurbita moschata])
HSP 1 Score: 758.1 bits (1956), Expect = 4.0e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. NCBI nr
Match:
XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 756.1 bits (1951), Expect = 1.5e-214
Identity = 357/403 (88.59%), Postives = 382/403 (94.79%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQP GYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DG+L++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH S+SEDEKEFW+WSWE+LALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. NCBI nr
Match:
XP_023553815.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 756.1 bits (1951), Expect = 1.5e-214
Identity = 357/403 (88.59%), Postives = 382/403 (94.79%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQP GYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DG+L++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH S+SEDEKEFW+WSWE+LALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match:
Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)
HSP 1 Score: 503.8 bits (1296), Expect = 1.8e-141
Identity = 228/368 (61.96%), Postives = 293/368 (79.62%), Query Frame = 0
Query: 38 SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
S +LC+ L+ PA Y C EH +QTKDG++L LQRV+S L Q GPP+LL HGLFMA
Sbjct: 27 SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86
Query: 98 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
GD WFLNS +SLGFILAD+GFDVW+GNVRGTR+S+GH++LS+ +KEFW+WSW++LA+YD
Sbjct: 87 GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146
Query: 158 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 217
AEMI Y+ S++N K+++VGHSQGTIMS AALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206
Query: 218 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSR 277
V MV +HLD +++A G H++NF+SD +L+D LC+ ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266
Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSL 337
+ + +YEPHPSS KN+ HLFQMIR+GTF++YDYG KNLR YG KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326
Query: 338 PLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFF 397
P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L AKEDVY M++FF
Sbjct: 327 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 386
Query: 398 KSLGKSSS 406
++ KSSS
Sbjct: 387 RAKVKSSS 392
BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match:
Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)
HSP 1 Score: 293.1 bits (749), Expect = 4.8e-78
Identity = 144/367 (39.24%), Postives = 224/367 (61.04%), Query Frame = 0
Query: 43 LCSQLVQPAGYPCAEHKVQTKDGFLLGLQRV-----SSSDGDLKRQQGPPILLLHGLFMA 102
+C+ V GY C EH V T+DG++L +QR+ + GD ++Q P+L+ HG+ +
Sbjct: 51 ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110
Query: 103 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 162
G +W LN A Q+L ILAD GFDVW+GN RGTR+S H L+ ++ FWNW+W+EL YD
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170
Query: 163 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 222
M D+I+ LT +K++ +GHS GT++ A+ ++ + +V +AA+LSP++YL H+T +
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230
Query: 223 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRL-VNCINILSSITGENCCLNGS 282
+ L G+ E N KS + + +C + ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290
Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGML-KNLRVYGQRKPPAFDLSRIPK 342
DLF EP +S KN+ HL Q +R +Y+YG +N++ YGQ PPA+++S IP
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350
Query: 343 SLPLWMVYGGNDELSDWTDLQHTIQELK----SVPELVYLENYGHVDFILSVKAKEDVYD 399
LPL+ YGG D L+D D++ + + K + ++++Y H DFI+ V AK+ VY+
Sbjct: 351 ELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYN 410
BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match:
P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)
HSP 1 Score: 201.1 bits (510), Expect = 2.5e-50
Identity = 118/362 (32.60%), Postives = 198/362 (54.70%), Query Frame = 0
Query: 45 SQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKR-QQGPPILLLHGLFMAGDAWFL 104
SQ++ GYPC E++V T+DG++LG+ R+ + + + P + L HGL + W
Sbjct: 35 SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94
Query: 105 NSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMID 164
N SL F+LAD G+DVW+GN RG WS ++ S D EFW +S++E+A YD I+
Sbjct: 95 NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154
Query: 165 YINSLTNR-KVYVVGHSQGTIMSLAAL-TQPDIAKKVEAAALLSPISYLDHITAPLVRL- 224
+I T + K++ VGHSQGT + A T P +AKK++ L+P++ + + +PL ++
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKIS 214
Query: 225 MVDIHLDTVILA-------TGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCL 284
+ L ++ T F + E+LD LC N + I +N L
Sbjct: 215 FIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCS---NTLFIFCGFDKKN--L 274
Query: 285 NGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGM-LKNLRVYGQRKPPAFDLSR 344
N SRFD++ + P +S ++ H Q++R G F +++G +N+ Y Q+ PP +D+S
Sbjct: 275 NVSRFDVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSA 334
Query: 345 IPKSLPLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDP 395
+ + +W GGND L+D D+ + +L ++ + Y H+DFI ++ A ++VY+
Sbjct: 335 MTVPVAVW--NGGNDILADPQDVAMLLPKLSNLLFHKEILAYNHLDFIWAMDAPQEVYNE 389
BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match:
Q9CPP7 (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)
HSP 1 Score: 197.6 bits (501), Expect = 2.7e-49
Identity = 127/368 (34.51%), Postives = 201/368 (54.62%), Query Frame = 0
Query: 45 SQLVQPAGYPCAEHKVQTKDGFLLGLQRV----SSSDGDLKRQQGPPILLLHGLFMAGDA 104
SQ++ GYP E++V T+DG++LG+ R+ +S+ KR P L HGL +
Sbjct: 35 SQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKR---PVAYLQHGLIASATN 94
Query: 105 WFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAE 164
W N SL FILAD G+DVW+GN RG WS ++ S D EFW +S++E+A YD
Sbjct: 95 WITNLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPA 154
Query: 165 MIDYINSLTNR-KVYVVGHSQGTIMSLAAL-TQPDIAKKVEAAALLSPISYLDHITAPL- 224
ID+I T + K++ VGHSQGT + A T P +AKK++ L+P++ + + +P
Sbjct: 155 TIDFIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFK 214
Query: 225 -VRLMVDIHLDTVILATGFHELNFKSDW-GT-----ELLDDLCDRLVNCINILSSITGEN 284
+ L+ L + F N+ + GT ELLD LC N + I +N
Sbjct: 215 KISLIPKFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCS---NALFIFCGFDKKN 274
Query: 285 CCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGM-LKNLRVYGQRKPPAFD 344
LN SRFD++ + P +S ++L H Q+ + G Y++G L+N+ Y Q+ PP +D
Sbjct: 275 --LNVSRFDVYLGHNPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYD 334
Query: 345 LSRIPKSLPLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLEN---YGHVDFILSVKAK 395
+S + + +W GG+D L+D D+ + +L P L+Y + Y H+DFI ++ A
Sbjct: 335 VSAMTVPIAVW--NGGHDILADPQDVAMLLPKL---PNLLYHKEILPYNHLDFIWAMDAP 389
BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match:
O61866 (Lipase lipl-5 OS=Caenorhabditis elegans OX=6239 GN=lipl-5 PE=2 SV=1)
HSP 1 Score: 195.3 bits (495), Expect = 1.4e-48
Identity = 118/366 (32.24%), Postives = 189/366 (51.64%), Query Frame = 0
Query: 46 QLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQG--PPILLLHGLFMAGDAWFL 105
Q+++ GYP + V T DG++L + R+ ++ G P + + HGL A W +
Sbjct: 31 QIIERWGYPAMIYTVATDDGYILEMHRIPFGKTNVTWPNGKRPVVFMQHGLLCASSDWVV 90
Query: 106 NSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMID 165
N QS GF+ AD GFDVW+GN+RG +S H L FW+WSW+E+A YD MI+
Sbjct: 91 NLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNAMIN 150
Query: 166 YINSLTNR-KVYVVGHSQGTIMSLAALTQPD--IAKKVEAAALLSPISYLDHITAPL--V 225
++ +T + VY +GHSQGT+ + L++ D AKK++ L+PI + HI L
Sbjct: 151 HVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFLSFF 210
Query: 226 RLMVDIHLDTVILATGFHELNFKSDWGTEL-LDDLCDRLV----NCINILSSITG-ENCC 285
+ D G E ++W +L D+C L C N+L I G E+
Sbjct: 211 ANYFSLEFDGWFDIFGAGEF-LPNNWAMKLAAKDICGGLKVEADLCDNVLFLIAGPESDQ 270
Query: 286 LNGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSR 345
N +R ++ ++P +S +N+ H QM+ G YD+G N + YGQ PP +D +
Sbjct: 271 WNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQANPPEYDFTA 330
Query: 346 IPKSLPLWMVYGGNDELSDWTDL-QHTIQELKS--VPELVYLENYGHVDFILSVKAKEDV 396
I K +++ + D L+D D+ + + L V + +L +Y H+DF ++A +D+
Sbjct: 331 I-KGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTWGLRAPDDI 390
BLAST of Lag0014578 vs. ExPASy TrEMBL
Match:
A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)
HSP 1 Score: 760.0 bits (1961), Expect = 5.1e-216
Identity = 361/406 (88.92%), Postives = 385/406 (94.83%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKSSSL 407
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406
BLAST of Lag0014578 vs. ExPASy TrEMBL
Match:
A0A6J1GL56 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)
HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. ExPASy TrEMBL
Match:
A0A6J1GMQ3 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)
HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSADDS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403
BLAST of Lag0014578 vs. ExPASy TrEMBL
Match:
A0A6J1I8F7 (triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=4 SV=1)
HSP 1 Score: 753.4 bits (1944), Expect = 4.8e-214
Identity = 356/403 (88.34%), Postives = 379/403 (94.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSA+DS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYIN LT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+ KNLRVYGQRKPPAFDLS IPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KS GKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSFGKS 403
BLAST of Lag0014578 vs. ExPASy TrEMBL
Match:
A0A6J1I5U4 (Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1)
HSP 1 Score: 753.4 bits (1944), Expect = 4.8e-214
Identity = 356/403 (88.34%), Postives = 379/403 (94.04%), Query Frame = 0
Query: 1 MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
MAKPL ++FLL FF LHSA+DS RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1 MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60
Query: 61 QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61 QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120
Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYIN LT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180
Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240
Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
IR+GTFSRYDYG+ KNLRVYGQRKPPAFDLS IPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360
Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KS GKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSFGKS 403
BLAST of Lag0014578 vs. TAIR 10
Match:
AT2G15230.1 (lipase 1 )
HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-142
Identity = 228/368 (61.96%), Postives = 293/368 (79.62%), Query Frame = 0
Query: 38 SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
S +LC+ L+ PA Y C EH +QTKDG++L LQRV+S L Q GPP+LL HGLFMA
Sbjct: 27 SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86
Query: 98 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
GD WFLNS +SLGFILAD+GFDVW+GNVRGTR+S+GH++LS+ +KEFW+WSW++LA+YD
Sbjct: 87 GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146
Query: 158 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 217
AEMI Y+ S++N K+++VGHSQGTIMS AALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206
Query: 218 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSR 277
V MV +HLD +++A G H++NF+SD +L+D LC+ ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266
Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSL 337
+ + +YEPHPSS KN+ HLFQMIR+GTF++YDYG KNLR YG KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326
Query: 338 PLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFF 397
P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L AKEDVY M++FF
Sbjct: 327 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 386
Query: 398 KSLGKSSS 406
++ KSSS
Sbjct: 387 RAKVKSSS 392
BLAST of Lag0014578 vs. TAIR 10
Match:
AT5G14180.1 (Myzus persicae-induced lipase 1 )
HSP 1 Score: 293.1 bits (749), Expect = 3.4e-79
Identity = 144/367 (39.24%), Postives = 224/367 (61.04%), Query Frame = 0
Query: 43 LCSQLVQPAGYPCAEHKVQTKDGFLLGLQRV-----SSSDGDLKRQQGPPILLLHGLFMA 102
+C+ V GY C EH V T+DG++L +QR+ + GD ++Q P+L+ HG+ +
Sbjct: 51 ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110
Query: 103 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 162
G +W LN A Q+L ILAD GFDVW+GN RGTR+S H L+ ++ FWNW+W+EL YD
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170
Query: 163 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 222
M D+I+ LT +K++ +GHS GT++ A+ ++ + +V +AA+LSP++YL H+T +
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230
Query: 223 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRL-VNCINILSSITGENCCLNGS 282
+ L G+ E N KS + + +C + ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290
Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGML-KNLRVYGQRKPPAFDLSRIPK 342
DLF EP +S KN+ HL Q +R +Y+YG +N++ YGQ PPA+++S IP
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350
Query: 343 SLPLWMVYGGNDELSDWTDLQHTIQELK----SVPELVYLENYGHVDFILSVKAKEDVYD 399
LPL+ YGG D L+D D++ + + K + ++++Y H DFI+ V AK+ VY+
Sbjct: 351 ELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYN 410
BLAST of Lag0014578 vs. TAIR 10
Match:
AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 83.6 bits (205), Expect = 4.1e-16
Identity = 95/395 (24.05%), Postives = 153/395 (38.73%), Query Frame = 0
Query: 37 LSDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFM 96
++ + C ++ GYP +V T DG++L L+R+ +R + L HG+
Sbjct: 289 MNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIP------RRDARKAVFLQHGVLD 348
Query: 97 AGDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALY 156
+ W N S F D G+DV++GN RG S H++ + KEFW +S E
Sbjct: 349 SSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTE 408
Query: 157 DFAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK 216
D MI+ I+ + K+ + HS G L + I +K
Sbjct: 409 DIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEK 468
Query: 217 ---VEAAALLSPISYLD--------------HITAPLVRLMVDIHLDTVI-------LAT 276
+ LLSP + + I+ L R++ ++ T LA
Sbjct: 469 PHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLAR 528
Query: 277 GFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPS-SAK 336
FH N+ + L L L++ + + G++ G + P+ S +
Sbjct: 529 DFH--NYPA------LGGLVQTLMSYV-----VGGDSSNWVGVLGLPHYNMNDMPAVSFR 588
Query: 337 NLHHLFQMIRRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDEL 383
HL Q+ G F YDYG N+ VYG +P DL K +P+ +V G ND++
Sbjct: 589 VAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKV 648
BLAST of Lag0014578 vs. TAIR 10
Match:
AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 83.6 bits (205), Expect = 4.1e-16
Identity = 95/395 (24.05%), Postives = 153/395 (38.73%), Query Frame = 0
Query: 37 LSDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFM 96
++ + C ++ GYP +V T DG++L L+R+ +R + L HG+
Sbjct: 220 MNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIP------RRDARKAVFLQHGVLD 279
Query: 97 AGDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALY 156
+ W N S F D G+DV++GN RG S H++ + KEFW +S E
Sbjct: 280 SSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTE 339
Query: 157 DFAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK 216
D MI+ I+ + K+ + HS G L + I +K
Sbjct: 340 DIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEK 399
Query: 217 ---VEAAALLSPISYLD--------------HITAPLVRLMVDIHLDTVI-------LAT 276
+ LLSP + + I+ L R++ ++ T LA
Sbjct: 400 PHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLAR 459
Query: 277 GFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPS-SAK 336
FH N+ + L L L++ + + G++ G + P+ S +
Sbjct: 460 DFH--NYPA------LGGLVQTLMSYV-----VGGDSSNWVGVLGLPHYNMNDMPAVSFR 519
Query: 337 NLHHLFQMIRRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDEL 383
HL Q+ G F YDYG N+ VYG +P DL K +P+ +V G ND++
Sbjct: 520 VAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKV 579
BLAST of Lag0014578 vs. TAIR 10
Match:
AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )
HSP 1 Score: 74.3 bits (181), Expect = 2.5e-13
Identity = 95/386 (24.61%), Postives = 158/386 (40.93%), Query Frame = 0
Query: 38 SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
+D +T C ++ GYP +V T DG+ L L+R+ +R + L HG+ +
Sbjct: 289 TDTRT-CQDVITELGYPYEAIRVVTSDGYGLLLERIP------RRDARKAVYLQHGVMDS 348
Query: 98 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
W N S F D G+DV++GN RG S H+ + K+FW +S E A D
Sbjct: 349 SMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATED 408
Query: 158 FAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK- 217
MI+ I+ + K+ VV HS G L + I +K
Sbjct: 409 IPAMIEKIHEIKTSELKLYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKP 468
Query: 218 --VEAAALLSPISYLDHITAPLVRLMVD---IHLDTVI--LATGFHELNFKSDWGTELLD 277
+ LLSP + H + + +++ + L V+ + F+ + + LL+
Sbjct: 469 HRLSRLILLSPAGF--HYDSNMCFTLMEYTFLFLGPVLSRIVPAFY---IPTKFFRMLLN 528
Query: 278 DLCDRLVN------CINILSS--ITGENCCLNGSRFDLFFEYEPHPS-SAKNLHHLFQMI 337
L N + L S + G++ G + P S + HL Q+
Sbjct: 529 KLARDFHNYPAVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIK 588
Query: 338 RRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDELSDWTDLQHT 383
G F +DYG N+ VYG +P DL +P+ +V G D++ + ++
Sbjct: 589 HSGKFKMFDYGSSSANMDVYGSPEP--LDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKH 648
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022952782.1 | 1.1e-215 | 88.92 | triacylglycerol lipase 1 isoform X2 [Cucurbita moschata] | [more] |
XP_022952781.1 | 4.0e-215 | 89.33 | triacylglycerol lipase 1 isoform X1 [Cucurbita moschata] | [more] |
XP_022952784.1 | 4.0e-215 | 89.33 | triacylglycerol lipase 1 isoform X3 [Cucurbita moschata] | [more] |
XP_023553816.1 | 1.5e-214 | 88.59 | triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
XP_023553815.1 | 1.5e-214 | 88.59 | triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q71DJ5 | 1.8e-141 | 61.96 | Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1 | [more] |
Q67ZU1 | 4.8e-78 | 39.24 | Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1 | [more] |
P04634 | 2.5e-50 | 32.60 | Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1 | [more] |
Q9CPP7 | 2.7e-49 | 34.51 | Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1 | [more] |
O61866 | 1.4e-48 | 32.24 | Lipase lipl-5 OS=Caenorhabditis elegans OX=6239 GN=lipl-5 PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GLC0 | 5.1e-216 | 88.92 | Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1 | [more] |
A0A6J1GL56 | 1.9e-215 | 89.33 | Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1 | [more] |
A0A6J1GMQ3 | 1.9e-215 | 89.33 | Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1 | [more] |
A0A6J1I8F7 | 4.8e-214 | 88.34 | triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 ... | [more] |
A0A6J1I5U4 | 4.8e-214 | 88.34 | Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1 | [more] |