Lag0014578 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014578
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLipase
Locationchr12: 2258467 .. 2264235 (+)
RNA-Seq ExpressionLag0014578
SyntenyLag0014578
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGCCATTGACGACTCTGATATTTCTTCTCTACTTCTTCACTCTTCACTCTGCCGATGATTCCTTCGATCGTTCCGTCGAGTACTCGATTCTCCCTCGCGTTTTGTCCGACGAGAAGACCTTGTGCTCGCAACTCGTTCAACCTGCTGGCTATCCTTGCGCGGAGCATAAGGTACTCTGATTCTCTCTTCTCGCTGCTGATTTCTTGGTTGATTAAGTAGTGCGAATGGAAACGGACTTGGAATTGTTTGTGAAATGAGACTGTATTAGTGTTCATCGCTCTGTTCCGAGTGGAGAAACTGGAATGGATTTAGTCAACTGATTGGCTGTAATGCTTCTTGCTTGAGAGTTCGTTTGAGAGTTCTTTCTAGTTTCTCGTTTATTTTCTTTCCTGAGTTCAGAATGATGGAATGTTTTTCTTTGTATCCTTGGATTCGTTGTCCGTCCTTGATTGATTTGTTTCTCTATCGAGTTTCTGGATAAACTGATGAATTTGTACTGTTCTTTGTGTTGATGAATGAAAGTATAGGCATAGAGAATATATGCGAAGGAGAATGCTCGAATTTTCTCATCTGTATGATCTCTAATGCCAGAGAGCGCAGAGCTTTTATTTTATCAATTTCTTATTAGTCATTAGCTATTCGGACCGCAACGCATTTCTACCGTATAATTTTATTTAAAAAATGATATATTCTGTTATCATTCTCCTGAATTTTGAGGAAGGCTATCCATTACTGCAGAGTGGGCTGATGAATTGTGTTTATAGTGGTATACTTGACTACGGTAGTGCAGTGCTGACGGACTTGATTTTCTTTTAAATTTCTTAGGTTCAAACGAAGGATGGCTTTTTGTTAGGTCTTCAACGTGTATCTTCATCCGATGGAGATTTGAAAAGGCAACAAGGTCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGTGAGTCTTCTTAAAGTTTTGCAATATTGTAACTGTATTTCTAAAATCATTTCACTCTTTTATGGTATGTACCTATCAGCAATAGCTAGAAAATCGACAAGACTGCCTGGGTTTTGGGATCTTTATTTTATTTATTTCTCACAGCTATCTCAAACTTCTGTTTGAAAGAAAAGACTGTAGACAGCTGATATTTATTTTGCTATCCAGACTTAACTGTATCTCCTTTCATTGTAATTTTTAACTGTACTCTGTTAGACCCCCAATCATGAAGGTTTATTCCCAGATGGGTAAAGAGGGGAATTTAGTTCCAAATGTTGATAAGGAGGTTTAGTTTTAGTTGCTCATTTGTATTTGAAAGTTTGAATAGGGAGTTAGAAGGACTTAGTCGATAATGTGTTTCTCCTTCTTTTATGTTGCTTTGTGTTCTTTTTTTATTGGGCATTATGTTGTCTTGTGTCAAGTCAGTTTGTAAAGGTGTGGAGAGAAAGTTGACCCTCTTGAATTATCCTTTTGCTTAGAATTTCTTGCGTCTAGGAGATTAAGAGCTGGGTATTTTGAAACATGGATTGTAAACTTTGAATAATTTTGCAGACTGGGATCATGCTCACAGATAAGGGACATGGTTCCTACCACTAACTTTCCAAACCTTTCATTGTCTGAATTGCTGCATGTGCAGGCAGGCGATGCATGGTTTTTGAATTCTGCAGCACAATCATTAGGCTTTATTCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACGCGGTGGAGTCACGGGCATATCTCCTTATCAGAGGATGAGAAGGTGTTTGTAGATTACTTTATTATAAAATGCCTTGGGTAGGTTGTTACTTAAATGCAAAGAGAGAACATATTAATAGAAAGGCATTGTGTTGCATGTATCTAGAAAGTTTAATAATGATACTGATACAAGCATAGCATCTTGCTGCTCGTCCATGCTCAATGCTTTGAGGTGGAGATTATCATGCAGTCAATTATAGAGGTTCAAACAAATTAGAGTGAGGTTTTCTGGTGTTCGGCAGTAACATCACAGGTTATATTAAAAGGCACATTTGTGTTCATGTGCTAAAGTCTATTTTTTCTCAAATCTCTTCACTATATCGTCTGGTGGATTGCTGGTTCAAAAGCATCTTAGGACAATCTTGGAAGTAATGTTAACATAAATTGAATATGTTTAGTTTCTGTTTTTGACTCTCGTATATACTTACTGTAGGAATTTTGGAATTGGAGTTGGGAAGAATTGGCTCTGTATGATTTTGCAGAAATGATTGACTACATAAATTCATTGACAAACAGGAAAGTTTATGTTGTTGGGCATTCGCAGGTTTCATATCTTTTCTTTTACAAGATTGTTTATTTGAAATTTTGCTTGTTGCTCTTGTGTCATCTTCTGGCTTTTTTCACAGGGAACAATCATGTCTTTAGCTGCTCTTACCCAACCAGACATAGCAAAGAAAGTTGAAGCAGCTGCCCTTCTAAGTCCCATATCATACTTGGACCATATCACTGCACCATTAGTACGTCTAATGGTTGACATTCATCTTGATACGGTAATCCTGTTATTTTGTGATTAGCATTGTTAAATTTTATATTACTAATAGTTATAAGCTTATTCCATATATTTTTCTCAGCAGGTAATTCTTGCAACAGGATTTCATGAGCTTAACTTTAAAAGGTTGCCATGCTATGTCCAATTTTGGTTCAATTTTTAGCTTCAGCGTAGTTCATAATACTAATGTCTATGTTTCTTTATGCTACTTCAGTGATTGGGGAACAGAACTTTTAGATGATCTTTGTGACCGGCTTGTTAATTGCATCAATATTCTAAGTTCAATAACAGGTTCTCCCATTGCACATCGCTTCATGTTTCTTTTCTCTGATCTTAATTCCCTTTTCTTTACCTTCGAAGCCATTGCATAGGAAATTGTTACTAAAATGCTTTCTGCTAATGATTCTTTCTACCTAATTAAATGATTAATTTTTTTAAAAAAACTGTTTTTTTTTTAAAAACTGTTTTTTGCAATTCTTGAATTTGGAATCCAATATTGCTAATTTCTTTTCATTACTCTAATGTATACGACGTGGATCCTTATTGGATCATAATTTTATGTGTTTAGTGGGGTACATCTTATCTTAAAACTGAAAGGCTTAAAATGGAAAATGTTACTAATATTTACCAAGTGCTCCATATTTCATATGATAATGCTGCCAGAAAGCTTCTATCAGTGCAATTCATGTAATAGGCTACCAACTTACTAAGCTCCATTTGTTTTTTTTAAGAAGCCAAGCTCCATTTGTTATTCTGATGCGTATCTGCATTCTCATTCATTTATTTGAGCCTTAATTTTATATGCTATACTGTATTTCATTTTCAGGATTTAAGTGCCCTAGGTCGGGAAAATTTCTATTTTACTTTGTAAAATGACATGATCCTATAATGATAAATGCCAGGATTTATAAGGTCATGTTCTTCTTGTACTCGGAAAAGGATTGGTTATAACTAGAAAAGAAAGTCAATAATGAGTTGTTGATTGGAATGACAGTATGGAATATTGATTTATTCTTTATAGGGAAGCATCATGTTTGAATGTTCAATCTATGCAAATGCAGACACTTAAATTTTCTAACGACTAACGAGGCCTTATAATGCTGCAGGGGAGAATTGTTGCCTTAATGGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGAGTACGCTCATGCAGTATCTTCTCTCAGCTTCATGTTCTAGTGTACTGTGACTTTGATGAATCTATAAATTTTTTACCTGCAGTAATGTGTTGGGAAGTTAATTTTAGTGTTCCCTTGTGAATTCTCTTATCCAAAAAAAAAAAAAAGGTGTTCCCTTGTGAAGTCTAATATAAATCCTTGAACATTGAATCGTGAAACTTGCATCATACATTCCAAAACTCTTTCAAGAAACCTCCTAGAAAAGAACTCGCAAATTTGATCTTCTTCCTCACATTACTTCTTCAAGAAACCTCCAAGAGATATCCAAAAACCCATCAAAGCACAACCAAAAAGGATACTCAGCTTCCCACAAAGACCTTTTTTTTATCATTAACAGTTTCTTCCAGATACCAAATTTGCCAACTCTCTAGTCTCTACAATATTTTATTTCATGGTCTTACTCTTCTCAAACGCTTCTTTCATGACAAACAACGAATAGAGGTAGTATGAAAAATTTTGCATACTATATTACTAGAAATTTCCTTACAAAGAGACTTTCATATGCTCTTTAACTTATTTCGTTCAAGCCCTTTCTTGAGTGACTTCCACTGAACCCCTCACTTCCCACCAACTTTATTTCTAATTAAAGCCACCACTCTATTTCCAAAGCAACTTTTAGCAATAACCGAACTCGCTTGCCCATTCCACTTCCATGTCCATAAAATTCTTTTAACTAACTTCCCACCTAATGTCTCTTCAGTTGCCATTAATAAATTCCAGAACCACCATCTCCTTGAAAAAACCCCGAGGATCCTCCCATCCTTGATTGAATTTCTGGGACCTTCGCTTTCGACCCTTTCCCTTTTCCTTTCAATCAAAGCTTCAATTACTACTTGATATTTCCCTTCTTGTTTTCTTTCACATATGCTAGGTACAAACATCATAACTTATCATTCTCAAGCTGTTTATCTTTGCGGTTCGACTTTATAATATTGTACTTCATGTCAGAACATTAGTGATGGTTTAGGATCAGTTTTTCTGTTGTTAAATCTGTTGATGTTGTTGACAAAGTTATTACATATGCATGAGGATCCCCTTAGTTGTTAGACAATTAGCTCATGAAAATGTAAATACAGAAAGTGAAAGAAGGATACTTATGCCATATTATTAGATAACATGGGGTTCCATCTCAAATTCAATTGACAATGAGTGGGGTGACCATCTTATAAATTGTGAAGTCTCCTATTCTCTTTTCAATGTGAGATCCTCAACATGCCTCCTCAAGATGGTGCCTCTCATGGGTTCACCATTCTTGGATCAAATTCCAATTTTTTTTTCTTGGACCGGATACCCGTTTGAGTTTAATGGTCTCTGATACCATATTAGATAACATGTGGTTCTATCTCAAAACCAATTGGCAATGAGTGGGATGACCCTTGTATCTTATAAATTATGAGGTTTTCACTTCTCTTTTCAATGTGAGATCCTCAACACATATAACAGAACAAACAACTCCTTTGACAAGAGAAAAACTGATCCTACCCATCACTAAGGTCCTGACTTCAATACGTGACAACAATTTACTACACTGTAATTGAATTACTGGTTTTAAGAAGTTATATATTTTTCAAGTCAATAACTTGGTTGGGCAGATATCTTTTTCTTTCCTCCTGGCTGGAACAGGAGACAGTTTGAGACAGCTGATTTAAAATTTTAAATGCCCTTATCTTCTTTTTCCAGTGATCCGCAGGGGCACATTTTCCAGGTATGACTATGGAATGTTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGCATTCGATCTCAGCCGAATTCCTAAGTCGTTGCCGCTATGGATGGTGTACGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGCAGCACACGATCCAGGAGTTGAAGTCGGTACCGGAGTTGGTTTACCTTGAAAATTATGGTCATGTCGACTTCATTTTGAGCGTGAAAGCGAAGGAAGATGTGTATGATCCTATGCTCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

mRNA sequence

ATGGCGAAGCCATTGACGACTCTGATATTTCTTCTCTACTTCTTCACTCTTCACTCTGCCGATGATTCCTTCGATCGTTCCGTCGAGTACTCGATTCTCCCTCGCGTTTTGTCCGACGAGAAGACCTTGTGCTCGCAACTCGTTCAACCTGCTGGCTATCCTTGCGCGGAGCATAAGGTTCAAACGAAGGATGGCTTTTTGTTAGGTCTTCAACGTGTATCTTCATCCGATGGAGATTTGAAAAGGCAACAAGGTCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGCAGGCGATGCATGGTTTTTGAATTCTGCAGCACAATCATTAGGCTTTATTCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACGCGGTGGAGTCACGGGCATATCTCCTTATCAGAGGATGAGAAGGAATTTTGGAATTGGAGTTGGGAAGAATTGGCTCTGTATGATTTTGCAGAAATGATTGACTACATAAATTCATTGACAAACAGGAAAGTTTATGTTGTTGGGCATTCGCAGGGAACAATCATGTCTTTAGCTGCTCTTACCCAACCAGACATAGCAAAGAAAGTTGAAGCAGCTGCCCTTCTAAGTCCCATATCATACTTGGACCATATCACTGCACCATTAGTACGTCTAATGGTTGACATTCATCTTGATACGGTAATTCTTGCAACAGGATTTCATGAGCTTAACTTTAAAAGTGATTGGGGAACAGAACTTTTAGATGATCTTTGTGACCGGCTTGTTAATTGCATCAATATTCTAAGTTCAATAACAGGGGAGAATTGTTGCCTTAATGGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGATGATCCGCAGGGGCACATTTTCCAGGTATGACTATGGAATGTTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGCATTCGATCTCAGCCGAATTCCTAAGTCGTTGCCGCTATGGATGGTGTACGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGCAGCACACGATCCAGGAGTTGAAGTCGGTACCGGAGTTGGTTTACCTTGAAAATTATGGTCATGTCGACTTCATTTTGAGCGTGAAAGCGAAGGAAGATGTGTATGATCCTATGCTCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Coding sequence (CDS)

ATGGCGAAGCCATTGACGACTCTGATATTTCTTCTCTACTTCTTCACTCTTCACTCTGCCGATGATTCCTTCGATCGTTCCGTCGAGTACTCGATTCTCCCTCGCGTTTTGTCCGACGAGAAGACCTTGTGCTCGCAACTCGTTCAACCTGCTGGCTATCCTTGCGCGGAGCATAAGGTTCAAACGAAGGATGGCTTTTTGTTAGGTCTTCAACGTGTATCTTCATCCGATGGAGATTTGAAAAGGCAACAAGGTCCTCCAATTTTGCTCCTCCATGGACTGTTCATGGCAGGCGATGCATGGTTTTTGAATTCTGCAGCACAATCATTAGGCTTTATTCTTGCAGATAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACGCGGTGGAGTCACGGGCATATCTCCTTATCAGAGGATGAGAAGGAATTTTGGAATTGGAGTTGGGAAGAATTGGCTCTGTATGATTTTGCAGAAATGATTGACTACATAAATTCATTGACAAACAGGAAAGTTTATGTTGTTGGGCATTCGCAGGGAACAATCATGTCTTTAGCTGCTCTTACCCAACCAGACATAGCAAAGAAAGTTGAAGCAGCTGCCCTTCTAAGTCCCATATCATACTTGGACCATATCACTGCACCATTAGTACGTCTAATGGTTGACATTCATCTTGATACGGTAATTCTTGCAACAGGATTTCATGAGCTTAACTTTAAAAGTGATTGGGGAACAGAACTTTTAGATGATCTTTGTGACCGGCTTGTTAATTGCATCAATATTCTAAGTTCAATAACAGGGGAGAATTGTTGCCTTAATGGCTCACGTTTTGACTTATTTTTCGAATATGAACCACATCCATCATCAGCAAAAAACCTGCACCATCTTTTCCAGATGATCCGCAGGGGCACATTTTCCAGGTATGACTATGGAATGTTAAAGAATCTGAGAGTCTACGGTCAAAGGAAGCCTCCTGCATTCGATCTCAGCCGAATTCCTAAGTCGTTGCCGCTATGGATGGTGTACGGTGGCAACGACGAGTTGTCAGATTGGACAGATCTGCAGCACACGATCCAGGAGTTGAAGTCGGTACCGGAGTTGGTTTACCTTGAAAATTATGGTCATGTCGACTTCATTTTGAGCGTGAAAGCGAAGGAAGATGTGTATGATCCTATGCTCAAATTCTTTAAATCCTTGGGAAAGTCAAGTAGTTTGTAA

Protein sequence

MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKSSSL
Homology
BLAST of Lag0014578 vs. NCBI nr
Match: XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 760.0 bits (1961), Expect = 1.1e-215
Identity = 361/406 (88.92%), Postives = 385/406 (94.83%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKSSSL 407
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406

BLAST of Lag0014578 vs. NCBI nr
Match: XP_022952781.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 758.1 bits (1956), Expect = 4.0e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. NCBI nr
Match: XP_022952784.1 (triacylglycerol lipase 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 758.1 bits (1956), Expect = 4.0e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. NCBI nr
Match: XP_023553816.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 1.5e-214
Identity = 357/403 (88.59%), Postives = 382/403 (94.79%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQP GYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+L++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH S+SEDEKEFW+WSWE+LALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. NCBI nr
Match: XP_023553815.1 (triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 1.5e-214
Identity = 357/403 (88.59%), Postives = 382/403 (94.79%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQP GYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRALSDQKSLCSQLVQPVGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DG+L++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSRDGELEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH S+SEDEKEFW+WSWE+LALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSVSEDEKEFWDWSWEDLALYDLAGMIDYINSLTKRKIYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.8e-141
Identity = 228/368 (61.96%), Postives = 293/368 (79.62%), Query Frame = 0

Query: 38  SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
           S   +LC+ L+ PA Y C EH +QTKDG++L LQRV+S    L  Q GPP+LL HGLFMA
Sbjct: 27  SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86

Query: 98  GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
           GD WFLNS  +SLGFILAD+GFDVW+GNVRGTR+S+GH++LS+ +KEFW+WSW++LA+YD
Sbjct: 87  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146

Query: 158 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 217
            AEMI Y+ S++N K+++VGHSQGTIMS AALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206

Query: 218 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSR 277
           V  MV +HLD +++A G H++NF+SD   +L+D LC+  ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266

Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSL 337
            + + +YEPHPSS KN+ HLFQMIR+GTF++YDYG  KNLR YG  KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326

Query: 338 PLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFF 397
           P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKEDVY  M++FF
Sbjct: 327 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 386

Query: 398 KSLGKSSS 406
           ++  KSSS
Sbjct: 387 RAKVKSSS 392

BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 4.8e-78
Identity = 144/367 (39.24%), Postives = 224/367 (61.04%), Query Frame = 0

Query: 43  LCSQLVQPAGYPCAEHKVQTKDGFLLGLQRV-----SSSDGDLKRQQGPPILLLHGLFMA 102
           +C+  V   GY C EH V T+DG++L +QR+      +  GD  ++Q  P+L+ HG+ + 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110

Query: 103 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 162
           G +W LN A Q+L  ILAD GFDVW+GN RGTR+S  H  L+  ++ FWNW+W+EL  YD
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170

Query: 163 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 222
              M D+I+ LT +K++ +GHS GT++  A+ ++  +  +V +AA+LSP++YL H+T  +
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230

Query: 223 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRL-VNCINILSSITGENCCLNGS 282
             +     L       G+ E N KS    + +  +C +  ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290

Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGML-KNLRVYGQRKPPAFDLSRIPK 342
             DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP 
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350

Query: 343 SLPLWMVYGGNDELSDWTDLQHTIQELK----SVPELVYLENYGHVDFILSVKAKEDVYD 399
            LPL+  YGG D L+D  D++  + + K        + ++++Y H DFI+ V AK+ VY+
Sbjct: 351 ELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYN 410

BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.5e-50
Identity = 118/362 (32.60%), Postives = 198/362 (54.70%), Query Frame = 0

Query: 45  SQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKR-QQGPPILLLHGLFMAGDAWFL 104
           SQ++   GYPC E++V T+DG++LG+ R+     + +   + P + L HGL  +   W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 105 NSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMID 164
           N    SL F+LAD G+DVW+GN RG  WS  ++  S D  EFW +S++E+A YD    I+
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 165 YINSLTNR-KVYVVGHSQGTIMSLAAL-TQPDIAKKVEAAALLSPISYLDHITAPLVRL- 224
           +I   T + K++ VGHSQGT +   A  T P +AKK++    L+P++ + +  +PL ++ 
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKIS 214

Query: 225 MVDIHLDTVILA-------TGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCL 284
            +   L  ++         T F +         E+LD LC    N + I      +N  L
Sbjct: 215 FIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCS---NTLFIFCGFDKKN--L 274

Query: 285 NGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGM-LKNLRVYGQRKPPAFDLSR 344
           N SRFD++  + P  +S ++  H  Q++R G F  +++G   +N+  Y Q+ PP +D+S 
Sbjct: 275 NVSRFDVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSA 334

Query: 345 IPKSLPLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDP 395
           +   + +W   GGND L+D  D+   + +L ++     +  Y H+DFI ++ A ++VY+ 
Sbjct: 335 MTVPVAVW--NGGNDILADPQDVAMLLPKLSNLLFHKEILAYNHLDFIWAMDAPQEVYNE 389

BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match: Q9CPP7 (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.7e-49
Identity = 127/368 (34.51%), Postives = 201/368 (54.62%), Query Frame = 0

Query: 45  SQLVQPAGYPCAEHKVQTKDGFLLGLQRV----SSSDGDLKRQQGPPILLLHGLFMAGDA 104
           SQ++   GYP  E++V T+DG++LG+ R+     +S+   KR   P   L HGL  +   
Sbjct: 35  SQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKR---PVAYLQHGLIASATN 94

Query: 105 WFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAE 164
           W  N    SL FILAD G+DVW+GN RG  WS  ++  S D  EFW +S++E+A YD   
Sbjct: 95  WITNLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPA 154

Query: 165 MIDYINSLTNR-KVYVVGHSQGTIMSLAAL-TQPDIAKKVEAAALLSPISYLDHITAPL- 224
            ID+I   T + K++ VGHSQGT +   A  T P +AKK++    L+P++ + +  +P  
Sbjct: 155 TIDFIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFK 214

Query: 225 -VRLMVDIHLDTVILATGFHELNFKSDW-GT-----ELLDDLCDRLVNCINILSSITGEN 284
            + L+    L  +     F   N+   + GT     ELLD LC    N + I      +N
Sbjct: 215 KISLIPKFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCS---NALFIFCGFDKKN 274

Query: 285 CCLNGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGM-LKNLRVYGQRKPPAFD 344
             LN SRFD++  + P  +S ++L H  Q+ + G    Y++G  L+N+  Y Q+ PP +D
Sbjct: 275 --LNVSRFDVYLGHNPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYD 334

Query: 345 LSRIPKSLPLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLEN---YGHVDFILSVKAK 395
           +S +   + +W   GG+D L+D  D+   + +L   P L+Y +    Y H+DFI ++ A 
Sbjct: 335 VSAMTVPIAVW--NGGHDILADPQDVAMLLPKL---PNLLYHKEILPYNHLDFIWAMDAP 389

BLAST of Lag0014578 vs. ExPASy Swiss-Prot
Match: O61866 (Lipase lipl-5 OS=Caenorhabditis elegans OX=6239 GN=lipl-5 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.4e-48
Identity = 118/366 (32.24%), Postives = 189/366 (51.64%), Query Frame = 0

Query: 46  QLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQG--PPILLLHGLFMAGDAWFL 105
           Q+++  GYP   + V T DG++L + R+     ++    G  P + + HGL  A   W +
Sbjct: 31  QIIERWGYPAMIYTVATDDGYILEMHRIPFGKTNVTWPNGKRPVVFMQHGLLCASSDWVV 90

Query: 106 NSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMID 165
           N   QS GF+ AD GFDVW+GN+RG  +S  H  L      FW+WSW+E+A YD   MI+
Sbjct: 91  NLPDQSAGFLFADAGFDVWLGNMRGNTYSMKHKDLKPSHSAFWDWSWDEMATYDLNAMIN 150

Query: 166 YINSLTNR-KVYVVGHSQGTIMSLAALTQPD--IAKKVEAAALLSPISYLDHITAPL--V 225
           ++  +T +  VY +GHSQGT+   + L++ D   AKK++    L+PI  + HI   L   
Sbjct: 151 HVLEVTGQDSVYYMGHSQGTLTMFSHLSKDDGSFAKKIKKFFALAPIGSVKHIKGFLSFF 210

Query: 226 RLMVDIHLDTVILATGFHELNFKSDWGTEL-LDDLCDRLV----NCINILSSITG-ENCC 285
                +  D      G  E    ++W  +L   D+C  L      C N+L  I G E+  
Sbjct: 211 ANYFSLEFDGWFDIFGAGEF-LPNNWAMKLAAKDICGGLKVEADLCDNVLFLIAGPESDQ 270

Query: 286 LNGSRFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSR 345
            N +R  ++  ++P  +S +N+ H  QM+  G    YD+G   N + YGQ  PP +D + 
Sbjct: 271 WNQTRVPVYATHDPAGTSTQNIVHWMQMVHHGGVPAYDWGTKTNKKKYGQANPPEYDFTA 330

Query: 346 IPKSLPLWMVYGGNDELSDWTDL-QHTIQELKS--VPELVYLENYGHVDFILSVKAKEDV 396
           I K   +++ +   D L+D  D+  + +  L    V +  +L +Y H+DF   ++A +D+
Sbjct: 331 I-KGTKIYLYWSDADWLADTPDVPDYLLTRLNPAIVAQNNHLPDYNHLDFTWGLRAPDDI 390

BLAST of Lag0014578 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.1e-216
Identity = 361/406 (88.92%), Postives = 385/406 (94.83%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKSSSL 407
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406

BLAST of Lag0014578 vs. ExPASy TrEMBL
Match: A0A6J1GL56 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. ExPASy TrEMBL
Match: A0A6J1GMQ3 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 360/403 (89.33%), Postives = 383/403 (95.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSADDS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+LKNLRVYGQRKPPAFDLSRIPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KSLGKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKS 403

BLAST of Lag0014578 vs. ExPASy TrEMBL
Match: A0A6J1I8F7 (triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 4.8e-214
Identity = 356/403 (88.34%), Postives = 379/403 (94.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSA+DS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYIN LT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+ KNLRVYGQRKPPAFDLS IPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KS GKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSFGKS 403

BLAST of Lag0014578 vs. ExPASy TrEMBL
Match: A0A6J1I5U4 (Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 4.8e-214
Identity = 356/403 (88.34%), Postives = 379/403 (94.04%), Query Frame = 0

Query: 1   MAKPLTTLIFLLYFFTLHSADDSFDRSVEYSILPRVLSDEKTLCSQLVQPAGYPCAEHKV 60
           MAKPL  ++FLL FF LHSA+DS  RS+EYSILPR LSD+K+LCSQLVQPAGYPCAEH++
Sbjct: 1   MAKPLMAMVFLLCFFALHSANDSSYRSLEYSILPRALSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMAGDAWFLNSAAQSLGFILADNGFD 120
           QTKDGFLLGLQRVSS DGDL++Q+GPPILLLHGLFM GDAWFLNSA +SLGFILADNGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSATESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYDFAEMIDYINSLTNRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSEDEKEFW+WSWEELALYD A MIDYIN LT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINPLTKRKIYVVGHSQ 180

Query: 181 GTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDIHLDTVILATGFHELNF 240
           GTIMS AALTQPDIAKKVEAAALLSPISYL+HITAPLVRLMVD HLDT+ILA GFHELNF
Sbjct: 181 GTIMSFAALTQPDIAKKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300
           KSDWGT LLDDLCDRLVNCIN+LSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM
Sbjct: 241 KSDWGTALLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSLPLWMVYGGNDELSDWTDLQHTIQ 360
           IR+GTFSRYDYG+ KNLRVYGQRKPPAFDLS IPKSLPLWM YGG+DELSDWTDLQHTI+
Sbjct: 301 IRKGTFSRYDYGLFKNLRVYGQRKPPAFDLSHIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFFKSLGKS 404
           ELKSVPELVYLENYGHVDFILS+KAKEDVYDPM+KF KS GKS
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSFGKS 403

BLAST of Lag0014578 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-142
Identity = 228/368 (61.96%), Postives = 293/368 (79.62%), Query Frame = 0

Query: 38  SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
           S   +LC+ L+ PA Y C EH +QTKDG++L LQRV+S    L  Q GPP+LL HGLFMA
Sbjct: 27  SPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRL--QSGPPVLLQHGLFMA 86

Query: 98  GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
           GD WFLNS  +SLGFILAD+GFDVW+GNVRGTR+S+GH++LS+ +KEFW+WSW++LA+YD
Sbjct: 87  GDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWDWSWQDLAMYD 146

Query: 158 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 217
            AEMI Y+ S++N K+++VGHSQGTIMS AALTQP +A+ VEAAALL PISYLDH+TAPL
Sbjct: 147 LAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPISYLDHVTAPL 206

Query: 218 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSR 277
           V  MV +HLD +++A G H++NF+SD   +L+D LC+  ++C + L+SITG NCC N S+
Sbjct: 207 VERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSITGTNCCFNASK 266

Query: 278 FDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGMLKNLRVYGQRKPPAFDLSRIPKSL 337
            + + +YEPHPSS KN+ HLFQMIR+GTF++YDYG  KNLR YG  KPP F LS IP SL
Sbjct: 267 IEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPEFILSHIPASL 326

Query: 338 PLWMVYGGNDELSDWTDLQHTIQELKSVPELVYLENYGHVDFILSVKAKEDVYDPMLKFF 397
           P+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKEDVY  M++FF
Sbjct: 327 PMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKEDVYKHMIQFF 386

Query: 398 KSLGKSSS 406
           ++  KSSS
Sbjct: 387 RAKVKSSS 392

BLAST of Lag0014578 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 293.1 bits (749), Expect = 3.4e-79
Identity = 144/367 (39.24%), Postives = 224/367 (61.04%), Query Frame = 0

Query: 43  LCSQLVQPAGYPCAEHKVQTKDGFLLGLQRV-----SSSDGDLKRQQGPPILLLHGLFMA 102
           +C+  V   GY C EH V T+DG++L +QR+      +  GD  ++Q  P+L+ HG+ + 
Sbjct: 51  ICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQ--PVLIQHGILVD 110

Query: 103 GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 162
           G +W LN A Q+L  ILAD GFDVW+GN RGTR+S  H  L+  ++ FWNW+W+EL  YD
Sbjct: 111 GMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYD 170

Query: 163 FAEMIDYINSLTNRKVYVVGHSQGTIMSLAALTQPDIAKKVEAAALLSPISYLDHITAPL 222
              M D+I+ LT +K++ +GHS GT++  A+ ++  +  +V +AA+LSP++YL H+T  +
Sbjct: 171 LPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVI 230

Query: 223 VRLMVDIHLDTVILATGFHELNFKSDWGTELLDDLCDRL-VNCINILSSITGENCCLNGS 282
             +     L       G+ E N KS    + +  +C +  ++C +++S ITG+NCCLN S
Sbjct: 231 GDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNAS 290

Query: 283 RFDLFFEYEPHPSSAKNLHHLFQMIRRGTFSRYDYGML-KNLRVYGQRKPPAFDLSRIPK 342
             DLF   EP  +S KN+ HL Q +R     +Y+YG   +N++ YGQ  PPA+++S IP 
Sbjct: 291 TIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPH 350

Query: 343 SLPLWMVYGGNDELSDWTDLQHTIQELK----SVPELVYLENYGHVDFILSVKAKEDVYD 399
            LPL+  YGG D L+D  D++  + + K        + ++++Y H DFI+ V AK+ VY+
Sbjct: 351 ELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYN 410

BLAST of Lag0014578 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-16
Identity = 95/395 (24.05%), Postives = 153/395 (38.73%), Query Frame = 0

Query: 37  LSDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFM 96
           ++ +   C  ++   GYP    +V T DG++L L+R+       +R     + L HG+  
Sbjct: 289 MNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIP------RRDARKAVFLQHGVLD 348

Query: 97  AGDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALY 156
           +   W  N    S  F   D G+DV++GN RG   S  H++ +   KEFW +S  E    
Sbjct: 349 SSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTE 408

Query: 157 DFAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK 216
           D   MI+ I+ +                      K+  + HS G    L  +    I +K
Sbjct: 409 DIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEK 468

Query: 217 ---VEAAALLSPISYLD--------------HITAPLVRLMVDIHLDTVI-------LAT 276
              +    LLSP  + +               I+  L R++   ++ T         LA 
Sbjct: 469 PHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLAR 528

Query: 277 GFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPS-SAK 336
            FH  N+ +      L  L   L++ +     + G++    G      +     P+ S +
Sbjct: 529 DFH--NYPA------LGGLVQTLMSYV-----VGGDSSNWVGVLGLPHYNMNDMPAVSFR 588

Query: 337 NLHHLFQMIRRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDEL 383
              HL Q+   G F  YDYG    N+ VYG  +P   DL    K   +P+ +V G ND++
Sbjct: 589 VAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKV 648

BLAST of Lag0014578 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 83.6 bits (205), Expect = 4.1e-16
Identity = 95/395 (24.05%), Postives = 153/395 (38.73%), Query Frame = 0

Query: 37  LSDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFM 96
           ++ +   C  ++   GYP    +V T DG++L L+R+       +R     + L HG+  
Sbjct: 220 MNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIP------RRDARKAVFLQHGVLD 279

Query: 97  AGDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALY 156
           +   W  N    S  F   D G+DV++GN RG   S  H++ +   KEFW +S  E    
Sbjct: 280 SSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINEHGTE 339

Query: 157 DFAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK 216
           D   MI+ I+ +                      K+  + HS G    L  +    I +K
Sbjct: 340 DIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRKIKEK 399

Query: 217 ---VEAAALLSPISYLD--------------HITAPLVRLMVDIHLDTVI-------LAT 276
              +    LLSP  + +               I+  L R++   ++ T         LA 
Sbjct: 400 PHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLNKLAR 459

Query: 277 GFHELNFKSDWGTELLDDLCDRLVNCINILSSITGENCCLNGSRFDLFFEYEPHPS-SAK 336
            FH  N+ +      L  L   L++ +     + G++    G      +     P+ S +
Sbjct: 460 DFH--NYPA------LGGLVQTLMSYV-----VGGDSSNWVGVLGLPHYNMNDMPAVSFR 519

Query: 337 NLHHLFQMIRRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDEL 383
              HL Q+   G F  YDYG    N+ VYG  +P   DL    K   +P+ +V G ND++
Sbjct: 520 VAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRNDKV 579

BLAST of Lag0014578 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.5e-13
Identity = 95/386 (24.61%), Postives = 158/386 (40.93%), Query Frame = 0

Query: 38  SDEKTLCSQLVQPAGYPCAEHKVQTKDGFLLGLQRVSSSDGDLKRQQGPPILLLHGLFMA 97
           +D +T C  ++   GYP    +V T DG+ L L+R+       +R     + L HG+  +
Sbjct: 289 TDTRT-CQDVITELGYPYEAIRVVTSDGYGLLLERIP------RRDARKAVYLQHGVMDS 348

Query: 98  GDAWFLNSAAQSLGFILADNGFDVWIGNVRGTRWSHGHISLSEDEKEFWNWSWEELALYD 157
              W  N    S  F   D G+DV++GN RG   S  H+  +   K+FW +S  E A  D
Sbjct: 349 SMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVKKNISSKDFWRYSINEHATED 408

Query: 158 FAEMIDYINSLTNR-------------------KVYVVGHSQGTIMSLAALTQPDIAKK- 217
              MI+ I+ +                      K+ VV HS G    L  +    I +K 
Sbjct: 409 IPAMIEKIHEIKTSELKLYQPTMEEVVNEDQPYKLCVVSHSLGGAAVLMYVITRKIEEKP 468

Query: 218 --VEAAALLSPISYLDHITAPLVRLMVD---IHLDTVI--LATGFHELNFKSDWGTELLD 277
             +    LLSP  +  H  + +   +++   + L  V+  +   F+     + +   LL+
Sbjct: 469 HRLSRLILLSPAGF--HYDSNMCFTLMEYTFLFLGPVLSRIVPAFY---IPTKFFRMLLN 528

Query: 278 DLCDRLVN------CINILSS--ITGENCCLNGSRFDLFFEYEPHPS-SAKNLHHLFQMI 337
            L     N       +  L S  + G++    G      +     P  S +   HL Q+ 
Sbjct: 529 KLARDFHNYPAVGGLVQTLMSYVVGGDSSNWVGVMGLPHYNMNDMPGISFRVAQHLAQIK 588

Query: 338 RRGTFSRYDYGMLK-NLRVYGQRKPPAFDLSRIPK--SLPLWMVYGGNDELSDWTDLQHT 383
             G F  +DYG    N+ VYG  +P   DL        +P+ +V G  D++   + ++  
Sbjct: 589 HSGKFKMFDYGSSSANMDVYGSPEP--LDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKH 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022952782.11.1e-21588.92triacylglycerol lipase 1 isoform X2 [Cucurbita moschata][more]
XP_022952781.14.0e-21589.33triacylglycerol lipase 1 isoform X1 [Cucurbita moschata][more]
XP_022952784.14.0e-21589.33triacylglycerol lipase 1 isoform X3 [Cucurbita moschata][more]
XP_023553816.11.5e-21488.59triacylglycerol lipase 1 isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023553815.11.5e-21488.59triacylglycerol lipase 1 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q71DJ51.8e-14161.96Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Q67ZU14.8e-7839.24Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
P046342.5e-5032.60Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Q9CPP72.7e-4934.51Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1[more]
O618661.4e-4832.24Lipase lipl-5 OS=Caenorhabditis elegans OX=6239 GN=lipl-5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GLC05.1e-21688.92Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1GL561.9e-21589.33Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1GMQ31.9e-21589.33Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
A0A6J1I8F74.8e-21488.34triacylglycerol lipase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470951 ... [more]
A0A6J1I5U44.8e-21488.34Lipase OS=Cucurbita maxima OX=3661 GN=LOC111470951 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G15230.11.3e-14261.96lipase 1 [more]
AT5G14180.13.4e-7939.24Myzus persicae-induced lipase 1 [more]
AT1G73920.14.1e-1624.05alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.24.1e-1624.05alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.12.5e-1324.61alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 35..399
e-value: 1.3E-114
score: 385.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 44..398
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 3..402
e-value: 4.0E-125
score: 415.8
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 45..104
e-value: 5.3E-18
score: 64.4
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 27..400
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 27..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014578.1Lag0014578.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:2000033 regulation of seed dormancy process
biological_process GO:0010029 regulation of seed germination
biological_process GO:0009739 response to gibberellin
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004806 triglyceride lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds