Lag0014517 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014517
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionpathogenesis-related protein 1-like
Locationchr12: 1582302 .. 1582790 (+)
RNA-Seq ExpressionLag0014517
SyntenyLag0014517
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTTCTCAAGCTCTCCCTCTCCCTCTTCTGTGTCTTGAGCTTCACCACACTTCCCTCTGCCCTCGCTCAAGACTCACCTCAGGACTTCGTCAATGCCCACAACGCAGCCCGCGCCCAAGTCGGCGTCGGCCCCGTGCAATGGGACGAAAGGGTGGCAAGCTACGCTCGACAGTATGCCAACCAACGCATCAACGACTGTCGATTGATGCACTCCGGTGGCCCGTATGGGGAGAATATCGCATGGGGTAGCCCCGACTTGTCGGGCACCGCGGCGGTTCGGATGTGGGTCGACGAGAAGCAATTCTACGATCATGGCACCAACACATGTGCTGCTGGCAAGGTGTGCGGCCATTACACACAGGTGGTGTGGAGAAACTCGGTGAGAATTGGATGTGCAAAAGTGAGATGCACCAACGGCCGTGGTACTTTCATCACTTGCAACTATGATCCCAGAGGCAATTTCATAGGGCAGAGGCCATATTGA

mRNA sequence

ATGGATCTTCTCAAGCTCTCCCTCTCCCTCTTCTGTGTCTTGAGCTTCACCACACTTCCCTCTGCCCTCGCTCAAGACTCACCTCAGGACTTCGTCAATGCCCACAACGCAGCCCGCGCCCAAGTCGGCGTCGGCCCCGTGCAATGGGACGAAAGGGTGGCAAGCTACGCTCGACAGTATGCCAACCAACGCATCAACGACTGTCGATTGATGCACTCCGGTGGCCCGTATGGGGAGAATATCGCATGGGGTAGCCCCGACTTGTCGGGCACCGCGGCGGTTCGGATGTGGGTCGACGAGAAGCAATTCTACGATCATGGCACCAACACATGTGCTGCTGGCAAGGTGTGCGGCCATTACACACAGGTGGTGTGGAGAAACTCGGTGAGAATTGGATGTGCAAAAGTGAGATGCACCAACGGCCGTGGTACTTTCATCACTTGCAACTATGATCCCAGAGGCAATTTCATAGGGCAGAGGCCATATTGA

Coding sequence (CDS)

ATGGATCTTCTCAAGCTCTCCCTCTCCCTCTTCTGTGTCTTGAGCTTCACCACACTTCCCTCTGCCCTCGCTCAAGACTCACCTCAGGACTTCGTCAATGCCCACAACGCAGCCCGCGCCCAAGTCGGCGTCGGCCCCGTGCAATGGGACGAAAGGGTGGCAAGCTACGCTCGACAGTATGCCAACCAACGCATCAACGACTGTCGATTGATGCACTCCGGTGGCCCGTATGGGGAGAATATCGCATGGGGTAGCCCCGACTTGTCGGGCACCGCGGCGGTTCGGATGTGGGTCGACGAGAAGCAATTCTACGATCATGGCACCAACACATGTGCTGCTGGCAAGGTGTGCGGCCATTACACACAGGTGGTGTGGAGAAACTCGGTGAGAATTGGATGTGCAAAAGTGAGATGCACCAACGGCCGTGGTACTTTCATCACTTGCAACTATGATCCCAGAGGCAATTTCATAGGGCAGAGGCCATATTGA

Protein sequence

MDLLKLSLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQYANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGHYTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY
Homology
BLAST of Lag0014517 vs. NCBI nr
Match: XP_038887491.1 (pathogenesis-related protein 1-like [Benincasa hispida])

HSP 1 Score: 313.2 bits (801), Expect = 1.4e-81
Identity = 143/162 (88.27%), Postives = 155/162 (95.68%), Query Frame = 0

Query: 1   MDLLKLSLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQY 60
           MDLLKLSLSLFCVLSFT LPS+LAQDSPQD++NAHNAARAQVGVGPVQWDERVAS+ARQY
Sbjct: 1   MDLLKLSLSLFCVLSFTMLPSSLAQDSPQDYLNAHNAARAQVGVGPVQWDERVASFARQY 60

Query: 61  ANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGHY 120
           ANQRINDC+L+HSGGPYGENIAWG PDLSGTAAV+MWV+EKQFY++GTNTCAAGKVCGHY
Sbjct: 61  ANQRINDCKLIHSGGPYGENIAWGWPDLSGTAAVQMWVNEKQFYNYGTNTCAAGKVCGHY 120

Query: 121 TQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           TQVVWR SVRIGCAKV+CTN  GTFI CNYDPRGN IGQ+PY
Sbjct: 121 TQVVWRKSVRIGCAKVKCTNNGGTFIICNYDPRGNIIGQKPY 162

BLAST of Lag0014517 vs. NCBI nr
Match: XP_008455064.1 (PREDICTED: pathogenesis-related protein PRB1-2-like [Cucumis melo] >KAA0031384.1 pathogenesis-related protein PRB1-2-like [Cucumis melo var. makuwa] >TYK06836.1 pathogenesis-related protein PRB1-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 307.4 bits (786), Expect = 7.4e-80
Identity = 142/164 (86.59%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 1   MDLLK--LSLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYAR 60
           MD+LK  LSLSLFCVL+F  LPS+LAQDSP+DFVNAHNAARAQVGVGPV WDERVAS+AR
Sbjct: 1   MDVLKLSLSLSLFCVLTFIMLPSSLAQDSPRDFVNAHNAARAQVGVGPVHWDERVASFAR 60

Query: 61  QYANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCG 120
           QYANQRINDCRL+HSGGPYGENIAWG PDLSGTAAV+MWV+EKQFY++G+NTCAAGKVCG
Sbjct: 61  QYANQRINDCRLVHSGGPYGENIAWGMPDLSGTAAVQMWVNEKQFYNYGSNTCAAGKVCG 120

Query: 121 HYTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           HYTQVVWRNSVRIGCAKVRCTN RGTFI CNY+PRGN IGQ+PY
Sbjct: 121 HYTQVVWRNSVRIGCAKVRCTNNRGTFIICNYEPRGNIIGQKPY 164

BLAST of Lag0014517 vs. NCBI nr
Match: XP_023553946.1 (pathogenesis-related protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 144/163 (88.34%), Postives = 149/163 (91.41%), Query Frame = 0

Query: 1   MDLLKLSLSLFCV-LSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQ 60
           MDL KLSLSLF V LSF    S+LAQDSPQDFVNAHNAARAQVGVGPVQWDERVAS+ARQ
Sbjct: 1   MDLFKLSLSLFYVGLSFAMFRSSLAQDSPQDFVNAHNAARAQVGVGPVQWDERVASFARQ 60

Query: 61  YANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGH 120
           YANQRINDCRL+HSGGPYGENIAWGSPDLSGT AVRMWVDEKQFY+   NTCAAGKVCGH
Sbjct: 61  YANQRINDCRLVHSGGPYGENIAWGSPDLSGTNAVRMWVDEKQFYNPSANTCAAGKVCGH 120

Query: 121 YTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           YTQVVWRNSVRIGCAKVRCTN RGTFI CNYDPRGN IGQRP+
Sbjct: 121 YTQVVWRNSVRIGCAKVRCTNNRGTFIICNYDPRGNIIGQRPF 163

BLAST of Lag0014517 vs. NCBI nr
Match: XP_022972506.1 (pathogenesis-related protein PR-1 type-like [Cucurbita maxima])

HSP 1 Score: 302.4 bits (773), Expect = 2.4e-78
Identity = 142/163 (87.12%), Postives = 150/163 (92.02%), Query Frame = 0

Query: 1   MDLLKLSLSLF-CVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQ 60
           MDL KLSLSLF  VLSF    S+LAQDSPQDFVNAHNAARAQVGVGP++WDERVAS+ARQ
Sbjct: 1   MDLFKLSLSLFYVVLSFAMFHSSLAQDSPQDFVNAHNAARAQVGVGPIRWDERVASFARQ 60

Query: 61  YANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGH 120
           YANQRINDCRL+HSGGPYGENIAWGSPDLSGT AVRMWVDEKQFY+  TNTCAAGKVCGH
Sbjct: 61  YANQRINDCRLVHSGGPYGENIAWGSPDLSGTNAVRMWVDEKQFYNPSTNTCAAGKVCGH 120

Query: 121 YTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           YTQVVWRNSVRIGCAKVRCTN RGTFITCNYDP GN +GQRP+
Sbjct: 121 YTQVVWRNSVRIGCAKVRCTNNRGTFITCNYDPPGNVVGQRPF 163

BLAST of Lag0014517 vs. NCBI nr
Match: XP_022952868.1 (pathogenesis-related protein 1-like [Cucurbita moschata])

HSP 1 Score: 301.6 bits (771), Expect = 4.1e-78
Identity = 142/163 (87.12%), Postives = 149/163 (91.41%), Query Frame = 0

Query: 1   MDLLKLSLSLF-CVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQ 60
           MDL KLSLS+F  VLSF    S+LAQDSPQDFVNAHNAARAQVGVGPVQWDERVAS+ARQ
Sbjct: 1   MDLFKLSLSIFYVVLSFAMFRSSLAQDSPQDFVNAHNAARAQVGVGPVQWDERVASFARQ 60

Query: 61  YANQRINDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGH 120
           YANQRINDCRL+HSGGPYGENIAWGSPDLSGT AVRMWVDEKQFY+   NTCAAG+VCGH
Sbjct: 61  YANQRINDCRLVHSGGPYGENIAWGSPDLSGTNAVRMWVDEKQFYNPSANTCAAGRVCGH 120

Query: 121 YTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           YTQVVWRNSVRIGCAKVRCTN RGTFI CNYDPRGN IGQRP+
Sbjct: 121 YTQVVWRNSVRIGCAKVRCTNNRGTFIICNYDPRGNIIGQRPF 163

BLAST of Lag0014517 vs. ExPASy Swiss-Prot
Match: P35793 (Pathogenesis-related protein PRB1-3 OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.0e-55
Identity = 100/164 (60.98%), Postives = 121/164 (73.78%), Query Frame = 0

Query: 1   MDLLKLSLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQY 60
           M   KL + L   +S   +  + AQ+SPQD+V+ HNAARA VGVG V W  ++ ++A+ Y
Sbjct: 1   MQTPKLVILLALAMSAAMVNLSQAQNSPQDYVSPHNAARAAVGVGAVSWSTKLQAFAQNY 60

Query: 61  ANQRINDCRLMHSGGPYGENIAWGS--PDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCG 120
           ANQRINDC+L HSGGPYGENI WGS   D   + AV  WV EK+ YD+G+NTCAAGKVCG
Sbjct: 61  ANQRINDCKLQHSGGPYGENIFWGSAGADWKASDAVNSWVSEKKDYDYGSNTCAAGKVCG 120

Query: 121 HYTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           HYTQVVWR S  IGCA+V C N RG FITCNY+PRGN +GQ+PY
Sbjct: 121 HYTQVVWRASTSIGCARVVCNNNRGVFITCNYEPRGNIVGQKPY 164

BLAST of Lag0014517 vs. ExPASy Swiss-Prot
Match: P33154 (Pathogenesis-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g14610 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-55
Identity = 99/155 (63.87%), Postives = 118/155 (76.13%), Query Frame = 0

Query: 8   LSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQYANQRIND 67
           L +F  L    +  + AQDSPQD++  HN AR  VGVGP+QWDERVA+YAR YA Q   +
Sbjct: 10  LIVFVALVGALVLPSKAQDSPQDYLRVHNQARGAVGVGPMQWDERVAAYARSYAEQLRGN 69

Query: 68  CRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGHYTQVVWRN 127
           CRL+HSGGPYGEN+AWGS DLSG +AV MWV EK  Y++  NTC    VCGHYTQVVWR 
Sbjct: 70  CRLIHSGGPYGENLAWGSGDLSGVSAVNMWVSEKANYNYAANTC--NGVCGHYTQVVWRK 129

Query: 128 SVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           SVR+GCAKVRC NG GT I+CNYDPRGN++ ++PY
Sbjct: 130 SVRLGCAKVRCNNG-GTIISCNYDPRGNYVNEKPY 161

BLAST of Lag0014517 vs. ExPASy Swiss-Prot
Match: Q05968 (Pathogenesis-related protein 1 OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.7e-55
Identity = 99/164 (60.37%), Postives = 122/164 (74.39%), Query Frame = 0

Query: 1   MDLLKLSLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQY 60
           M   KL++ L   ++   +  + AQ+SPQD+V+ HNAAR+ VGVG V W  ++ ++A+ Y
Sbjct: 1   MQTPKLAILLALAMAAAMVNLSQAQNSPQDYVSPHNAARSAVGVGAVSWSTKLQAFAQNY 60

Query: 61  ANQRINDCRLMHSGGPYGENIAWGS--PDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCG 120
           ANQRINDC+L HSGGPYGENI WGS   D   + AV  WV EK+ YD+G+NTCAAGKVCG
Sbjct: 61  ANQRINDCKLQHSGGPYGENIFWGSAGADWKASDAVNSWVSEKKDYDYGSNTCAAGKVCG 120

Query: 121 HYTQVVWRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           HYTQVVWR S  IGCA+V C N RG FITCNY+PRGN IGQ+PY
Sbjct: 121 HYTQVVWRASTSIGCARVVCNNNRGVFITCNYEPRGNIIGQKPY 164

BLAST of Lag0014517 vs. ExPASy Swiss-Prot
Match: P11670 (Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum OX=4097 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.0e-55
Identity = 100/158 (63.29%), Postives = 124/158 (78.48%), Query Frame = 0

Query: 6   LSLSLFCVLSFTTL-PSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQYANQR 65
           L+  + C ++F  L  S+ AQ+SPQD++N HNAAR QVGVGP+ WD R+A+YA+ YANQR
Sbjct: 4   LTTIVACFITFAILIHSSKAQNSPQDYLNPHNAARRQVGVGPMTWDNRLAAYAQNYANQR 63

Query: 66  INDCRLMHSGGPYGENIAWGSPDLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGHYTQVV 125
           I DC ++HS GPYGEN+A   P L+   AV+MWVDEK+FYD+ +N+C  G VCGHYTQVV
Sbjct: 64  IGDCGMIHSHGPYGENLAAAFPQLNAAGAVKMWVDEKRFYDYNSNSCVGG-VCGHYTQVV 123

Query: 126 WRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           WRNSVR+GCA+VR  NG   FITCNYDP GNFIGQRP+
Sbjct: 124 WRNSVRLGCARVRSNNG-WFFITCNYDPPGNFIGQRPF 159

BLAST of Lag0014517 vs. ExPASy Swiss-Prot
Match: Q41359 (Pathogenesis-related protein PR-1 type OS=Sambucus nigra OX=4202 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 6.6e-55
Identity = 102/158 (64.56%), Postives = 124/158 (78.48%), Query Frame = 0

Query: 7   SLSLFCVLSFTTLPSALAQDSPQDFVNAHNAARAQVGVGPVQWDERVASYARQYANQRIN 66
           S++L CV++   +  ++AQ+SPQD+V+AHNAAR+ V VGPV WDE VA++ARQYA  R  
Sbjct: 11  SVALVCVVALVMVQYSVAQNSPQDYVDAHNAARSAVNVGPVTWDESVAAFARQYAQSRAG 70

Query: 67  DCRLMHSGGP-YGENIAWGSP-DLSGTAAVRMWVDEKQFYDHGTNTCAAGKVCGHYTQVV 126
           DCRL+HSG P YGEN+A+GS  +L+G  AV MWV E+  Y+  TNTCA GKVCGHYTQVV
Sbjct: 71  DCRLVHSGDPRYGENLAFGSGFELTGRNAVDMWVAERNDYNPNTNTCAPGKVCGHYTQVV 130

Query: 127 WRNSVRIGCAKVRCTNGRGTFITCNYDPRGNFIGQRPY 163
           WRNSVRIGCA+VRC NG   FITCNY P GN+ GQRPY
Sbjct: 131 WRNSVRIGCARVRCNNG-AWFITCNYSPPGNYAGQRPY 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887491.11.4e-8188.27pathogenesis-related protein 1-like [Benincasa hispida][more]
XP_008455064.17.4e-8086.59PREDICTED: pathogenesis-related protein PRB1-2-like [Cucumis melo] >KAA0031384.1... [more]
XP_023553946.11.4e-7888.34pathogenesis-related protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_022972506.12.4e-7887.12pathogenesis-related protein PR-1 type-like [Cucurbita maxima][more]
XP_022952868.14.1e-7887.12pathogenesis-related protein 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P357931.0e-5560.98Pathogenesis-related protein PRB1-3 OS=Hordeum vulgare OX=4513 PE=2 SV=1[more]
P331541.3e-5563.87Pathogenesis-related protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g14610 PE=1... [more]
Q059681.7e-5560.37Pathogenesis-related protein 1 OS=Hordeum vulgare OX=4513 PE=2 SV=1[more]
P116703.0e-5563.29Basic form of pathogenesis-related protein 1 OS=Nicotiana tabacum OX=4097 PE=3 S... [more]
Q413596.6e-5564.56Pathogenesis-related protein PR-1 type OS=Sambucus nigra OX=4202 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001283Cysteine-rich secretory protein-relatedPRINTSPR00837V5TPXLIKEcoord: 93..106
score: 42.33
coord: 145..158
score: 53.08
coord: 47..65
score: 36.4
coord: 117..133
score: 66.19
IPR001283Cysteine-rich secretory protein-relatedPANTHERPTHR10334CYSTEINE-RICH SECRETORY PROTEIN-RELATEDcoord: 20..162
IPR014044CAP domainSMARTSM00198SCP_3coord: 26..158
e-value: 5.4E-66
score: 235.2
IPR014044CAP domainPFAMPF00188CAPcoord: 32..150
e-value: 1.6E-23
score: 83.9
IPR035940CAP superfamilyGENE3D3.40.33.10CAPcoord: 26..162
e-value: 8.9E-47
score: 160.8
IPR035940CAP superfamilySUPERFAMILY55797PR-1-likecoord: 12..162
NoneNo IPR availablePANTHERPTHR10334:SF488PATHOGENESIS-RELATED PROTEIN 1coord: 20..162
NoneNo IPR availableCDDcd05381CAP_PR-1coord: 28..162
e-value: 1.8152E-94
score: 267.963
IPR018244Allergen V5/Tpx-1-related, conserved sitePROSITEPS01010CRISP_2coord: 145..156
IPR018244Allergen V5/Tpx-1-related, conserved sitePROSITEPS01009CRISP_1coord: 118..128

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014517.1Lag0014517.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005615 extracellular space
cellular_component GO:0005576 extracellular region