Lag0014417 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014417
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDirigent protein
Locationchr12: 555839 .. 556387 (+)
RNA-Seq ExpressionLag0014417
SyntenyLag0014417
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGAAGAATAGAATCATATTCTGCTCTGCAATCTGCTTGGCCCTCCTAGCTGTTGTTCTCTTAGCCTTGCTTTCGCCGGTATCCCACAGAAAGCAGACCAATCAAGGCAGAAAACCGCCATGGGCGGACCTGTCCCTCTACATTCAACAGCCACATTCTAAAGCAAATGCTAGATCTAACAACATGCAGCCTGTAACGAGACCAGATTCTGGGGTTTTTGTCTTCCGACGAACGCTCACAAAGGGACCTGAGAACACTTCCCAGATCGTCGGAAATGCTCAAGGTTTCATTATTCCTAACGAACAGTTTGCGCATTCGTCGTTCAATATCATCTATCTCAGTTTTGACACACCAGAATATTCAGGCAGCCTGGGTGTCCATGCCAAACATATTGGGCATGAGAATGGAGAAGAAATGGCAGTGGTTGGGGGGACAGGTTCTTTTGCTTTTGCACAAGGGGTAGCTATTTTTCTACAGACAGAGAGGCAGGCATCTGTTACAGATACATCTTATCATTTAAAGCTTCAACTTCAATTCCCCAAGTGA

mRNA sequence

ATGATGAAGAATAGAATCATATTCTGCTCTGCAATCTGCTTGGCCCTCCTAGCTGTTGTTCTCTTAGCCTTGCTTTCGCCGGTATCCCACAGAAAGCAGACCAATCAAGGCAGAAAACCGCCATGGGCGGACCTGTCCCTCTACATTCAACAGCCACATTCTAAAGCAAATGCTAGATCTAACAACATGCAGCCTGTAACGAGACCAGATTCTGGGGTTTTTGTCTTCCGACGAACGCTCACAAAGGGACCTGAGAACACTTCCCAGATCGTCGGAAATGCTCAAGGTTTCATTATTCCTAACGAACAGTTTGCGCATTCGTCGTTCAATATCATCTATCTCAGTTTTGACACACCAGAATATTCAGGCAGCCTGGGTGTCCATGCCAAACATATTGGGCATGAGAATGGAGAAGAAATGGCAGTGGTTGGGGGGACAGGTTCTTTTGCTTTTGCACAAGGGGTAGCTATTTTTCTACAGACAGAGAGGCAGGCATCTGTTACAGATACATCTTATCATTTAAAGCTTCAACTTCAATTCCCCAAGTGA

Coding sequence (CDS)

ATGATGAAGAATAGAATCATATTCTGCTCTGCAATCTGCTTGGCCCTCCTAGCTGTTGTTCTCTTAGCCTTGCTTTCGCCGGTATCCCACAGAAAGCAGACCAATCAAGGCAGAAAACCGCCATGGGCGGACCTGTCCCTCTACATTCAACAGCCACATTCTAAAGCAAATGCTAGATCTAACAACATGCAGCCTGTAACGAGACCAGATTCTGGGGTTTTTGTCTTCCGACGAACGCTCACAAAGGGACCTGAGAACACTTCCCAGATCGTCGGAAATGCTCAAGGTTTCATTATTCCTAACGAACAGTTTGCGCATTCGTCGTTCAATATCATCTATCTCAGTTTTGACACACCAGAATATTCAGGCAGCCTGGGTGTCCATGCCAAACATATTGGGCATGAGAATGGAGAAGAAATGGCAGTGGTTGGGGGGACAGGTTCTTTTGCTTTTGCACAAGGGGTAGCTATTTTTCTACAGACAGAGAGGCAGGCATCTGTTACAGATACATCTTATCATTTAAAGCTTCAACTTCAATTCCCCAAGTGA

Protein sequence

MMKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARSNNMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEYSGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQFPK
Homology
BLAST of Lag0014417 vs. NCBI nr
Match: XP_038888051.1 (dirigent protein 8 [Benincasa hispida])

HSP 1 Score: 327.4 bits (838), Expect = 7.8e-86
Identity = 161/182 (88.46%), Postives = 169/182 (92.86%), Query Frame = 0

Query: 1   MMKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARS 60
           MMKNRIIFC+AICLA LAV+LLALLSPVSHRKQ    RKPPWADLSLYIQ+PHSK NARS
Sbjct: 25  MMKNRIIFCAAICLAFLAVILLALLSPVSHRKQAKHDRKPPWADLSLYIQRPHSKENARS 84

Query: 61  NNMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPE 120
           N M+PVT PDSG+FVFRRTLTKGPENTSQIVGNAQGFIIPNEQFA SSFNIIYLSFDTPE
Sbjct: 85  NKMKPVTEPDSGIFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFARSSFNIIYLSFDTPE 144

Query: 121 YSGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQF 180
           YSGSLGVHAK IGHEN EEM VVGGTGSFAFAQG+AIFLQTE+Q S+TDTSYHLKLQLQF
Sbjct: 145 YSGSLGVHAKRIGHENREEMTVVGGTGSFAFAQGIAIFLQTEKQTSITDTSYHLKLQLQF 204

Query: 181 PK 183
           PK
Sbjct: 205 PK 206

BLAST of Lag0014417 vs. NCBI nr
Match: XP_023511732.1 (uncharacterized protein LOC111776503 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.1 bits (819), Expect = 1.2e-83
Identity = 161/181 (88.95%), Postives = 167/181 (92.27%), Query Frame = 0

Query: 2   MKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARSN 61
           MKNRIIFC+AICLA+LAV+LLALLSPVSHRKQ    RKPPWADLSLYIQQPHSKAN+RSN
Sbjct: 1   MKNRIIFCAAICLAILAVILLALLSPVSHRKQAKHDRKPPWADLSLYIQQPHSKANSRSN 60

Query: 62  NMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEY 121
           NMQ V  PDSG+FVFRR LTKG ENTSQIVGNAQGFIIPNEQFA SSFNIIYLSFDT EY
Sbjct: 61  NMQLVPSPDSGIFVFRRMLTKGLENTSQIVGNAQGFIIPNEQFARSSFNIIYLSFDTLEY 120

Query: 122 SGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQFP 181
           SGSL VHAKHIGHEN EEM VVGGTGSFAFAQG+AIFLQTERQASVTDTSYHLKLQLQFP
Sbjct: 121 SGSLSVHAKHIGHENREEMTVVGGTGSFAFAQGIAIFLQTERQASVTDTSYHLKLQLQFP 180

Query: 182 K 183
           K
Sbjct: 181 K 181

BLAST of Lag0014417 vs. NCBI nr
Match: XP_022952599.1 (uncharacterized protein LOC111455239 [Cucurbita moschata])

HSP 1 Score: 320.1 bits (819), Expect = 1.2e-83
Identity = 160/181 (88.40%), Postives = 166/181 (91.71%), Query Frame = 0

Query: 2   MKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARSN 61
           MKNRIIFC+AICLA+LAV+LLALLSPVSHRKQ     KPPWADLSLYIQQPHSKAN+RSN
Sbjct: 1   MKNRIIFCAAICLAILAVILLALLSPVSHRKQAKHDSKPPWADLSLYIQQPHSKANSRSN 60

Query: 62  NMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEY 121
           NMQ V  PDSG+FVFRR LTKG ENTSQIVGNAQGFIIPNEQF  SSFNIIYLSFDTPEY
Sbjct: 61  NMQLVPSPDSGIFVFRRMLTKGLENTSQIVGNAQGFIIPNEQFGRSSFNIIYLSFDTPEY 120

Query: 122 SGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQFP 181
           SGSL VHAKHIGHEN EEM VVGGTGSFAFAQG+AIFLQTERQASVTDTSYHLKLQLQFP
Sbjct: 121 SGSLSVHAKHIGHENREEMTVVGGTGSFAFAQGIAIFLQTERQASVTDTSYHLKLQLQFP 180

Query: 182 K 183
           K
Sbjct: 181 K 181

BLAST of Lag0014417 vs. NCBI nr
Match: KAG6572477.1 (Dirigent protein 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012071.1 Dirigent protein 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 319.3 bits (817), Expect = 2.1e-83
Identity = 160/181 (88.40%), Postives = 167/181 (92.27%), Query Frame = 0

Query: 2   MKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARSN 61
           MKNRIIFC+AICLA+LAV+LLALLSPVSHRKQ    RKPPWADLSLYIQQP+SKAN+RSN
Sbjct: 1   MKNRIIFCAAICLAILAVILLALLSPVSHRKQAKHDRKPPWADLSLYIQQPNSKANSRSN 60

Query: 62  NMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEY 121
           NMQ V  PDSG+FVFRR LTKG ENTSQIVGNAQGFIIPNEQFA SSFNIIYLSFDTPEY
Sbjct: 61  NMQLVPSPDSGIFVFRRMLTKGLENTSQIVGNAQGFIIPNEQFARSSFNIIYLSFDTPEY 120

Query: 122 SGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQFP 181
           SGSL VHAKHIGHEN EEM VVGGTGSFAFAQG+AIFLQTERQASVTDT YHLKLQLQFP
Sbjct: 121 SGSLSVHAKHIGHENREEMTVVGGTGSFAFAQGIAIFLQTERQASVTDTCYHLKLQLQFP 180

Query: 182 K 183
           K
Sbjct: 181 K 181

BLAST of Lag0014417 vs. NCBI nr
Match: XP_022969428.1 (dirigent protein 8 [Cucurbita maxima])

HSP 1 Score: 319.3 bits (817), Expect = 2.1e-83
Identity = 160/181 (88.40%), Postives = 167/181 (92.27%), Query Frame = 0

Query: 2   MKNRIIFCSAICLALLAVVLLALLSPVSHRKQTNQGRKPPWADLSLYIQQPHSKANARSN 61
           MKNRIIFC+AICLA+LAV+LLALLSPVSHRKQ     KPPWADLSLYIQQPHSKAN+RS+
Sbjct: 1   MKNRIIFCAAICLAILAVILLALLSPVSHRKQAKHDGKPPWADLSLYIQQPHSKANSRSS 60

Query: 62  NMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEY 121
           NMQ V  PDSG+FVFRR LTKG ENTSQIVGNAQGFIIPNEQFA SSFNIIYLSFDTPEY
Sbjct: 61  NMQLVPSPDSGIFVFRRMLTKGLENTSQIVGNAQGFIIPNEQFARSSFNIIYLSFDTPEY 120

Query: 122 SGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFLQTERQASVTDTSYHLKLQLQFP 181
           SGSL VHAKHIGHEN EEM VVGGTGSFAFAQG+AIFLQTERQASVTDTSYHLKLQLQFP
Sbjct: 121 SGSLSVHAKHIGHENREEMTVVGGTGSFAFAQGIAIFLQTERQASVTDTSYHLKLQLQFP 180

Query: 182 K 183
           K
Sbjct: 181 K 181

BLAST of Lag0014417 vs. ExPASy Swiss-Prot
Match: A0A1V1FH01 (Pterocarpan synthase 1 OS=Glycyrrhiza echinata OX=46348 GN=PTS1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 9.8e-07
Identity = 37/101 (36.63%), Postives = 53/101 (52.48%), Query Frame = 0

Query: 58  ARSNNMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFD 117
           A  N     + P   V      LT GPE+ S++VG AQG      Q       ++ ++F 
Sbjct: 63  AEPNGKAKNSLPFGTVVAMDDPLTVGPESDSKLVGKAQGIYTSISQEEMGLMMVMTMAFS 122

Query: 118 TPEYSGS-LGVHAKH-IGHENGEEMAVVGGTGSFAFAQGVA 157
             E++GS L + A++ I  E   EMA+VGGTG+F FA+G A
Sbjct: 123 DGEFNGSTLSILARNMIMSEPVREMAIVGGTGAFRFARGYA 163

BLAST of Lag0014417 vs. ExPASy Swiss-Prot
Match: I1JNN8 (Pterocarpan synthase 1 OS=Glycine max OX=3847 GN=PTS1 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.1e-05
Identity = 37/112 (33.04%), Postives = 56/112 (50.00%), Query Frame = 0

Query: 47  LYIQQPHSKANARSNNMQPVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAH 106
           ++I +P+ KA            P   V      LT GPE  S++VG AQG      Q   
Sbjct: 58  VFIAEPNGKAKD--------ALPFGTVVAMDDPLTVGPEQDSKLVGKAQGIYTSISQEEM 117

Query: 107 SSFNIIYLSFDTPEYSGS-LGVHAKH-IGHENGEEMAVVGGTGSFAFAQGVA 157
               ++ ++F   +++GS + V  ++ I  E   EMA+VGGTG+F FA+G A
Sbjct: 118 GLMMVMTMAFTDGDFNGSTISVLGRNMIMSEPVREMAIVGGTGAFRFARGYA 161

BLAST of Lag0014417 vs. ExPASy Swiss-Prot
Match: Q9SUQ8 (Dirigent protein 6 OS=Arabidopsis thaliana OX=3702 GN=DIR6 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.9e-05
Identity = 48/158 (30.38%), Postives = 75/158 (47.47%), Query Frame = 0

Query: 15  ALLAVVLLALL---SPVSHRKQTNQGRKPPWADLSLY----IQQPHSKANARSNNMQPVT 74
           AL +  LL LL   + +S RK  +Q  K P    S Y    +    + ANA S  +  V+
Sbjct: 12  ALFSFFLLVLLFSDTVLSFRKTIDQ--KKPCKHFSFYFHDILYDGDNVANATSAAI--VS 71

Query: 75  RPDSGVF------VFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEY 134
            P  G F      +F   +T      S+ V  AQGF   + +   +S+    L F++ E+
Sbjct: 72  PPGLGNFKFGKFVIFDGPITMDKNYLSKPVARAQGFYFYDMKMDFNSWFSYTLVFNSTEH 131

Query: 135 SGSLGVHAKHIGHENGEEMAVVGGTGSFAFAQGVAIFL 160
            G+L +    +  E   +++VVGGTG F  A+G+A F+
Sbjct: 132 KGTLNIMGADLMMEPTRDLSVVGGTGDFFMARGIATFV 165

BLAST of Lag0014417 vs. ExPASy Swiss-Prot
Match: Q9SKQ2 (Dirigent protein 4 OS=Arabidopsis thaliana OX=3702 GN=DIR4 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 5.4e-05
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 0

Query: 69  PDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEYSG-SLGV 128
           P   +F     LT GP+  S+ +GNA+G  + + +   +    +   F + +++G S+ V
Sbjct: 73  PFGSLFALDDPLTVGPDPKSEKIGNARGMYVSSGKHVPTLTMYVDFGFTSGKFNGSSIAV 132

Query: 129 HAKHIGHENGEEMAVVGGTGSFAFAQGVA 157
            +++   E   E+AVVGG G F  A+GVA
Sbjct: 133 FSRNTITEKEREVAVVGGRGRFRMARGVA 161

BLAST of Lag0014417 vs. ExPASy Swiss-Prot
Match: F4J4N3 (Dirigent protein 17 OS=Arabidopsis thaliana OX=3702 GN=DIR17 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-04
Identity = 30/93 (32.26%), Postives = 46/93 (49.46%), Query Frame = 0

Query: 65  PVTRPDSGVFVFRRTLTKGPENTSQIVGNAQGFIIPNEQFAHSSFNIIYLSFDTPEYSGS 124
           P  +P+  + +    LT GPE TS+ VG AQG     +Q            F   E+SGS
Sbjct: 155 PGHKPEKLIPLVDDILTVGPEITSEEVGRAQGIFASADQNNFGLLMAFNFVFTKGEFSGS 214

Query: 125 -LGVHAKHIGHENGEEMAVVGGTGSFAFAQGVA 157
            + ++ ++       EM ++GGTG+F F +G A
Sbjct: 215 TVSMYGRNPIFSKVREMPIIGGTGAFRFGRGYA 247

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888051.17.8e-8688.46dirigent protein 8 [Benincasa hispida][more]
XP_023511732.11.2e-8388.95uncharacterized protein LOC111776503 [Cucurbita pepo subsp. pepo][more]
XP_022952599.11.2e-8388.40uncharacterized protein LOC111455239 [Cucurbita moschata][more]
KAG6572477.12.1e-8388.40Dirigent protein 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012071.... [more]
XP_022969428.12.1e-8388.40dirigent protein 8 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1V1FH019.8e-0736.63Pterocarpan synthase 1 OS=Glycyrrhiza echinata OX=46348 GN=PTS1 PE=1 SV=1[more]
I1JNN81.1e-0533.04Pterocarpan synthase 1 OS=Glycine max OX=3847 GN=PTS1 PE=2 SV=1[more]
Q9SUQ81.9e-0530.38Dirigent protein 6 OS=Arabidopsis thaliana OX=3702 GN=DIR6 PE=1 SV=1[more]
Q9SKQ25.4e-0531.46Dirigent protein 4 OS=Arabidopsis thaliana OX=3702 GN=DIR4 PE=2 SV=1[more]
F4J4N31.2e-0432.26Dirigent protein 17 OS=Arabidopsis thaliana OX=3702 GN=DIR17 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004265Dirigent proteinPFAMPF03018Dirigentcoord: 47..170
e-value: 4.0E-19
score: 68.9
IPR044859Allene oxide cyclase/Dirigent proteinGENE3D2.40.480.10coord: 49..182
e-value: 6.0E-8
score: 34.4
NoneNo IPR availablePANTHERPTHR21495NUCLEOPORIN-RELATEDcoord: 4..181
NoneNo IPR availablePANTHERPTHR21495:SF171DIRIGENT PROTEINcoord: 4..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014417.1Lag0014417.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0048046 apoplast