Lag0014098 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014098
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionFAD-binding Berberine family protein
Locationchr1: 55248776 .. 55250434 (-)
RNA-Seq ExpressionLag0014098
SyntenyLag0014098
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGTGTTTGCAAATCCTAAGTACAAATCTTTTGTGGGTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGTGTTGATTCAGCTTCACTTGAGGAGAGTTTCCTTCAATGTCTCAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATTTGAGGTATTTGGAGCCTTCAGTGCCAAAGCCTCTGTTTATCTTCACCCCATTGTTTGATTCCCATGTCCAAGCAGCTGTTATTTGCTCCAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTACGTGTCTGAAACTGAGACACCTTTCATAATTCTTGACCTTGCCAAGCTCCGATCTGTCAAAGTTGACATCGAGGGTAATAGTGCTTGGGTTCAGGCCGGGGCAACGATCGGCGAAGCTTATTACAGAATTGCTGAGAAAAGTAAGGTCCATGGCTTCCCTGCTGGCCTTTGCACAAGCCTGGGCGTTGGAGGTCATATAACAGGTGGTGCTTACGGTTCTATGATGCGAAAATACGGCCTCGGCGCTGACAATGTCGTTGATGCTCGAATTGTTGATGTAAATGGGAGAATTCTTGACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGAGGTAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCTTCAACTGTCACTGTTTTTACAGTTGCAAAGACATTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATTAGAGTCATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAAAAAACTGTGAGCACAGCTTACAATGCCCTCTTCTTGGGGGATGCAAACAGACTCCTCAAAGTTATGGGAGAGAGCTTTCCTGAATTGGGTTTGACAAGGAAAGATTGCATAGAAACAAGTTGGATCAAATCAGTTCTTTACATTGCTGGCTACCCAAGTGGAACTCCGCCAGAAGTGCTTCTCCAAGGGAAATCCACTTTCAAAAACTACTTCAAAGCCAAATCAGACTTTGTGAAAGACCCCATTCCAGAAACAGGCCTTGAAGGGCTGTGGAAAAGGCTGTTTGAAGAAGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGAAAAATTTCAGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAGATTCAGTACCTATCTACATGGCAAAAAGGAGATGGAAATCAAGACAAACACCTAGAGTGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAGCATATGTGAATTACAGGGATCTTGATTTAGGAATCAACAAGAAGGGAAACACGAGCTACATCAAGGCGATCGGTTGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTAAGAGTTAAGACTAAAATTGATCCTGATAACTTCTTCAGACATGAACAGAGCATTCCACCTATTCCGACTTCTGAAAGTTTAAGAAGCTTTAGCTCTTCCAAAAGGGGATTTGATTAG

mRNA sequence

ATGATGGTGTTTGCAAATCCTAAGTACAAATCTTTTGTGGGTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGTGTTGATTCAGCTTCACTTGAGGAGAGTTTCCTTCAATGTCTCAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATTTGAGGTATTTGGAGCCTTCAGTGCCAAAGCCTCTGTTTATCTTCACCCCATTGTTTGATTCCCATGTCCAAGCAGCTGTTATTTGCTCCAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTACGTGTCTGAAACTGAGACACCTTTCATAATTCTTGACCTTGCCAAGCTCCGATCTGTCAAAGTTGACATCGAGGGTAATAGTGCTTGGGTTCAGGCCGGGGCAACGATCGGCGAAGCTTATTACAGAATTGCTGAGAAAAGTAAGGTCCATGGCTTCCCTGCTGGCCTTTGCACAAGCCTGGGCGTTGGAGGTCATATAACAGGTGGTGCTTACGGTTCTATGATGCGAAAATACGGCCTCGGCGCTGACAATGTCGTTGATGCTCGAATTGTTGATGTAAATGGGAGAATTCTTGACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGAGGTAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCTTCAACTGTCACTGTTTTTACAGTTGCAAAGACATTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATTAGAGTCATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAAAAAACTGTGAGCACAGCTTACAATGCCCTCTTCTTGGGGGATGCAAACAGACTCCTCAAAGTTATGGGAGAGAGCTTTCCTGAATTGGGTTTGACAAGGAAAGATTGCATAGAAACAAGTTGGATCAAATCAGTTCTTTACATTGCTGGCTACCCAAGTGGAACTCCGCCAGAAGTGCTTCTCCAAGGGAAATCCACTTTCAAAAACTACTTCAAAGCCAAATCAGACTTTGTGAAAGACCCCATTCCAGAAACAGGCCTTGAAGGGCTGTGGAAAAGGCTGTTTGAAGAAGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGAAAAATTTCAGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAGATTCAGTACCTATCTACATGGCAAAAAGGAGATGGAAATCAAGACAAACACCTAGAGTGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAGCATATGTGAATTACAGGGATCTTGATTTAGGAATCAACAAGAAGGGAAACACGAGCTACATCAAGGCGATCGGTTGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTAAGAGTTAAGACTAAAATTGATCCTGATAACTTCTTCAGACATGAACAGAGCATTCCACCTATTCCGACTTCTGAAAGTTTAAGAAGCTTTAGCTCTTCCAAAAGGGGATTTGATTAG

Coding sequence (CDS)

ATGATGGTGTTTGCAAATCCTAAGTACAAATCTTTTGTGGGTTCTCTGTTTCTTGTCTTTCTGCTTTCATCTTCTTCTTGTGTTGATTCAGCTTCACTTGAGGAGAGTTTCCTTCAATGTCTCAATGAGAATTCTCAATTTTCTGTCCCTTACTCATCTTTTTGTGCTCCAAACAATGCTACATTTAATGCTCTTTTGCAATCAACTGCTCAGAATTTGAGGTATTTGGAGCCTTCAGTGCCAAAGCCTCTGTTTATCTTCACCCCATTGTTTGATTCCCATGTCCAAGCAGCTGTTATTTGCTCCAAACAGCTCAAGATTCATCTCAGAGTTCGTAGTGGAGGCCATGACTATGAGGGCCTCTCTTACGTGTCTGAAACTGAGACACCTTTCATAATTCTTGACCTTGCCAAGCTCCGATCTGTCAAAGTTGACATCGAGGGTAATAGTGCTTGGGTTCAGGCCGGGGCAACGATCGGCGAAGCTTATTACAGAATTGCTGAGAAAAGTAAGGTCCATGGCTTCCCTGCTGGCCTTTGCACAAGCCTGGGCGTTGGAGGTCATATAACAGGTGGTGCTTACGGTTCTATGATGCGAAAATACGGCCTCGGCGCTGACAATGTCGTTGATGCTCGAATTGTTGATGTAAATGGGAGAATTCTTGACAGAGAAGCCATGGGAGAGGACCTGTTTTGGGCTATTAGAGGAGGAGGAGGAGGTAGCTTTGGAATCATTCTGTGGTGGAAGCTAAATTTGGTTCCTGTTCCTTCAACTGTCACTGTTTTTACAGTTGCAAAGACATTAGAACAAGGTGCTACAAAAATCCTGTACAAATGGCAACAGGTTGCTGATAAGCTAGATGAAGATTTGTTCATTAGAGTCATTATAAATGTGGCTGCAAATGCTGATAAAAAGGGTCAAAAAACTGTGAGCACAGCTTACAATGCCCTCTTCTTGGGGGATGCAAACAGACTCCTCAAAGTTATGGGAGAGAGCTTTCCTGAATTGGGTTTGACAAGGAAAGATTGCATAGAAACAAGTTGGATCAAATCAGTTCTTTACATTGCTGGCTACCCAAGTGGAACTCCGCCAGAAGTGCTTCTCCAAGGGAAATCCACTTTCAAAAACTACTTCAAAGCCAAATCAGACTTTGTGAAAGACCCCATTCCAGAAACAGGCCTTGAAGGGCTGTGGAAAAGGCTGTTTGAAGAAGAAAGTCCATTGATGATATGGAATCCTTATGGTGGAATGATGGGAAAAATTTCAGAGACTGAGATTCCATTTCCTCATAGAAGAGGAGTCCTTTTCAAGATTCAGTACCTATCTACATGGCAAAAAGGAGATGGAAATCAAGACAAACACCTAGAGTGGATCAAGAAGCTTTACAATTACATGACACCTTATGTTTCTCAGCTTCCAAGAGAAGCATATGTGAATTACAGGGATCTTGATTTAGGAATCAACAAGAAGGGAAACACGAGCTACATCAAGGCGATCGGTTGGGGAACTCGGTATTTCAAAGAAAACTTTGGGAGATTGCTAAGAGTTAAGACTAAAATTGATCCTGATAACTTCTTCAGACATGAACAGAGCATTCCACCTATTCCGACTTCTGAAAGTTTAAGAAGCTTTAGCTCTTCCAAAAGGGGATTTGATTAG

Protein sequence

MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNATFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSESLRSFSSSKRGFD
Homology
BLAST of Lag0014098 vs. NCBI nr
Match: XP_038882764.1 (berberine bridge enzyme-like 15 [Benincasa hispida])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 530/552 (96.01%), Postives = 541/552 (98.01%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMVF NPKYKSFV SLFLVFLLSSSS VDSASLEESFLQCLNENSQFSVPYSSFCAPNNA
Sbjct: 1   MMVFTNPKYKSFVASLFLVFLLSSSSWVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQ+AVICSKQLKIHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLKIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSYVSE ETPFIILDLAKLRSVKVDI+GNSAWVQAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYVSEIETPFIILDLAKLRSVKVDIDGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
           TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           NADKKGQKTVSTAYNALFLGDA+RLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NADKKGQKTVSTAYNALFLGDASRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFE+ESPLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQKG+ NQDKHL+WIKKLYNYMTPYVSQLPRE YVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPRETYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTSES
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 552

BLAST of Lag0014098 vs. NCBI nr
Match: KAA0052471.1 (flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa] >TYK13351.1 flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1058.5 bits (2736), Expect = 1.9e-305
Identity = 516/552 (93.48%), Postives = 533/552 (96.56%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMV  NPKYKSFV  LFLVFLLSSSS VDS+SLEESFLQCLNENSQFSVPYSSFCAPNN+
Sbjct: 1   MMVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNS 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQ+AVICSKQL+IHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSY SE ETPFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
           TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           N    GQKTVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NG---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFE+ESPLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQKG+ NQDKHL+WIKKLYNYMTPYVSQLPREAYVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQS+PPIPTSE+
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEA 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 549

BLAST of Lag0014098 vs. NCBI nr
Match: XP_008439530.1 (PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.3e-305
Identity = 517/552 (93.66%), Postives = 532/552 (96.38%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMV  NPKYKSFV  LFLVFLLSSSS VDS SLEESFLQCLNENSQFSVPYSSFCAPNN+
Sbjct: 1   MMVSTNPKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNS 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQ+AVICSKQL+IHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSY SE ETPFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
           TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           N    GQKTVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NG---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFE+ESPLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQKG+ NQDKHL+WIKKLYNYMTPYVSQLPREAYVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTSE+
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEA 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 549

BLAST of Lag0014098 vs. NCBI nr
Match: XP_004134554.1 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8647767.1 hypothetical protein Csa_003323 [Cucumis sativus])

HSP 1 Score: 1047.7 bits (2708), Expect = 3.4e-302
Identity = 512/552 (92.75%), Postives = 528/552 (95.65%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMV  NPKYKSFV  LFLVFLLSSSS VDS+SLEESFLQCLNENSQFSVPYSSFCAPNNA
Sbjct: 1   MMVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNA 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPL DSHVQ+AVICSKQL+IHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLVDSHVQSAVICSKQLRIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSY SE ETPFIILDLAKLRS+KVDIE NSAW QAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYASEMETPFIILDLAKLRSIKVDIESNSAWAQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
            SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 PSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLD+DLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDDDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           N    GQKTVSTAYNALFLGDA+RLLKVMGESFPEL LTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NG---GQKTVSTAYNALFLGDASRLLKVMGESFPELSLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVK+PIPETGLEGLWKRLFE+E PLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKNPIPETGLEGLWKRLFEDEGPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQK + NQDKHL+WIKKLYNYMTPYVSQLPREAYVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKVEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTSE+
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEA 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 549

BLAST of Lag0014098 vs. NCBI nr
Match: XP_022146593.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.7e-301
Identity = 515/551 (93.47%), Postives = 526/551 (95.46%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61
           MVF  PKYKS VGSLFLVFLLSSS  VDSASLEESFLQCLNENSQFSVP+SSFCAPNNAT
Sbjct: 1   MVF--PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNAT 60

Query: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 121
           FNALLQSTAQNLRYLE SVPKPLFIFTP +DSHVQAAVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 61  FNALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 120

Query: 122 SYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCT 181
           SY SETET F+ILDLAKLRS+KVDIEGNSAWVQAGATIGE YYRIAEKSKVHGFPAGLCT
Sbjct: 121 SYASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180

Query: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGS 241
           SLGVGGHITGGAYG MMRKYGLGADNVVDARIVD NGRILDREAMGED+FWAIRGGGGGS
Sbjct: 181 SLGVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGS 240

Query: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300

Query: 302 ADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361
           ADKK  KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIE SWIKSVLYIAG+PSG
Sbjct: 301 ADKK--KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSG 360

Query: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKI 421
           TPPEVLLQGKSTFKNYFKAKSDFVK+PI ETGLEGLWKRLFEEESPL IWNPYGGMMGKI
Sbjct: 361 TPPEVLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKI 420

Query: 422 SETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYR 481
           SETEIPFPHRRG+LFKIQYL+ WQKGD NQ+KHLEWIKKLYNYMTPYVSQLPREAYVNYR
Sbjct: 421 SETEIPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYR 480

Query: 482 DLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSESL 541
           DLDLGINKKGNTSYI AIGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTS S 
Sbjct: 481 DLDLGINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASF 540

Query: 542 RSFSSSKRGFD 553
           RSF SS R FD
Sbjct: 541 RSFGSSSREFD 547

BLAST of Lag0014098 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 2.8e-214
Identity = 357/535 (66.73%), Postives = 442/535 (82.62%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPY-SSFCAPN-N 61
           M FA  K  +   +LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ N
Sbjct: 1   MAFAISKRNA---TLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQN 60

Query: 62  AT-FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDY 121
           AT F   L+STAQNLRYL PS PKP+FIF PL+++HVQAAV+C+K+L++HLR+RSGGHDY
Sbjct: 61  ATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDY 120

Query: 122 EGLSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAG 181
           EGLS+V+E ETPF+I+DL+KLR V VD++ NSAW  AGATIGE YYRI EKS+ HGFPAG
Sbjct: 121 EGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAG 180

Query: 182 LCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGG 241
           LC+SLG+GGH+ GGAYGSMMRK+GLGADNV+DARIVD NG+ILDR AMGED+FWAIRGGG
Sbjct: 181 LCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGG 240

Query: 242 GGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINV 301
           GGSFG+IL WK+ LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ 
Sbjct: 241 GGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISP 300

Query: 302 AANADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGY 361
           A+   K G +T+S +Y A FLGD+NRLL+VM +SFPELGLT+KDC E SWIKSV+YIAG+
Sbjct: 301 ASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGF 360

Query: 362 PSGTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMM 421
           P+   PE LL GKS FKN+FKAKSDFVK+PIP  GLEGLW+R  EE+SPL IWNPYGGMM
Sbjct: 361 PNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMM 420

Query: 422 GKISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYV 481
            +ISE+EIPFPHR G LFKIQ+LSTWQ G  ++++H++WI+++Y+YM  YVS+ PR+AYV
Sbjct: 421 SRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYV 480

Query: 482 NYRDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIP 534
           NYRDLDLG N +G T    A  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 481 NYRDLDLGTN-EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Lag0014098 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 1.2e-209
Identity = 351/523 (67.11%), Postives = 422/523 (80.69%), Query Frame = 0

Query: 17  FLVFLLSSSSC---VDSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQSTA 76
           FLV LL  S     +  +++++ F+ CL +NS  S P  SSF     N ++F   L+++A
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 77  QNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSETETP 136
           QNLRYL PS PKP FIF PL+++HVQAAV+C+K+LK+HLR+RSGGHDYEGLSYVSE ET 
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 137 FIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGGHIT 196
           F+I+DL+KLR + VDIE NSAWV AGA+IGE YYRI EKSK+HGFPAGLCTSLG+GGHI 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 197 GGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILWWKL 256
           GGAYGSMMRK+GLGADNV+DARIVD +G+IL+R AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 257 NLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 316
            LVPVP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K  ++T+
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 317 STAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 376
           ST+Y   FLGDANRLL+VM  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E LL G
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDG 371

Query: 377 KSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISETEIPFPH 436
           KS FKNYFKAKSD+V++PIP  GLEGLW++L EE+SPL IWNPYGGMM KI ETE PFPH
Sbjct: 372 KSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPH 431

Query: 437 RRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKK 496
           R G LFKIQ+L+ WQ G  ++ KH+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+N K
Sbjct: 432 RSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGK 491

Query: 497 GNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIP 534
           G+     A  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 492 GS----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Lag0014098 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.2e-202
Identity = 343/527 (65.09%), Postives = 423/527 (80.27%), Query Frame = 0

Query: 16  LFLVFLLSSSSCVDS-ASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQSTAQ 75
           L L F L + +   S ASL++ F+ C+  N+    P   +F AP  N + F+ +L+STAQ
Sbjct: 13  LVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQ 72

Query: 76  NLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSETETPF 135
           NLR+L+ S+PKP FIF+P+ +SHVQA++ICSK+L++HLRVRSGGHDYEGLSYVS+ + PF
Sbjct: 73  NLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPF 132

Query: 136 IILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGGHITG 195
           I++DL+K+R V ++I+ NSAWVQ+GAT+GE YYRIAEKSKVHGFPAGLC+SLG+GGHITG
Sbjct: 133 ILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITG 192

Query: 196 GAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILWWKLN 255
           GAYGSMMRKYGLGADNV+DA+IVD NG++LDR AMGED FWAIRGG GGSFGIIL WK+ 
Sbjct: 193 GAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIK 252

Query: 256 LVPVPSTVTVFTVAKTLEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 315
           LVPVP TVTVFTV KTL+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      G KTV
Sbjct: 253 LVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG---GNKTV 312

Query: 316 STAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 375
           +T+YNALFLG    L+ VM +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  VLLQG
Sbjct: 313 TTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQG 372

Query: 376 KSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISETEIPFP 435
           KS F K  FKAKSDFVK PIPE+GL+G++K+L +E+ PLMIWNPYGGMM KI E++IPFP
Sbjct: 373 KSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFP 432

Query: 436 HRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINK 495
           HR+GVLFK+QY+++W   D    +H+ WI+ LY+YMTPYVS  PREAYVNYRDLDLG N 
Sbjct: 433 HRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNT 492

Query: 496 KGNTSYIK-AIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPI 536
           K   + IK A  WG  YFK NF RL+ +K K+DP+NFFRHEQSIPP+
Sbjct: 493 KDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lag0014098 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.7e-187
Identity = 320/534 (59.93%), Postives = 404/534 (75.66%), Query Frame = 0

Query: 11  SFVGSLFLVFLLSSSSCVDSASLEESFLQCL--NENSQFSVPYSSFCAPNNAT-FNALLQ 70
           S +    L F   + +   S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 71  STAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSET 130
           STAQN RYL  ++PKP FIF P+ +SHVQA+VICSK+L+IH RVRSGGHDYEG+SYVS+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 131 ETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGG 190
           E PF+++DL+KLR + VDI+  SAWV+AGAT+GE YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 191 HITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILW 250
           HITGGAYGS+MRKYGL ADNV+DA+IVD NG++LDR +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 251 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKG 310
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N   K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 311 QKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 370
            KTV+ AY   FLG+   L++VM + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 371 EVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISE 430
           E+LLQ KS   K YFK KSDF K PIP  GLEG++K+L EE++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 431 TEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDL 490
           +EIPFPHR G  F IQY  +W   +   ++  +WI++LY YMTPYVS  PR+AYVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 491 DLGINKKGNTS-YIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIP 537
           DLG NK  + S +I+A  WG  YFK+NF RL+R+K+K+DPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Lag0014098 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.6e-185
Identity = 313/541 (57.86%), Postives = 398/541 (73.57%), Query Frame = 0

Query: 6   NPKYKSFVGSLFLVFLLSSSSCV----DSASLEESFLQCLNENSQFSVPYSSFC---APN 65
           N K    +  + +  L  S  C+     SAS+++ F+ C+  N+  S P        A N
Sbjct: 3   NSKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKN 62

Query: 66  NATFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDY 125
            + FN +L+STAQNL++L  S+PKP FIF P+  S VQA++ICSK+L IH RVRSGGHD+
Sbjct: 63  VSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDF 122

Query: 126 EGLSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAG 185
           E LSYVS  E PFI+LDL+KL+ + VDIE NSAWVQ GAT+GE YYRIAEKSK+HGFPAG
Sbjct: 123 EALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAG 182

Query: 186 LCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGG 245
           LCTS+G+GG++TGG YG++MRKYGL  DNV+D ++VD NG++LDR AMGEDLFWAIRGGG
Sbjct: 183 LCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGG 242

Query: 246 GGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGA-TKILYKWQQVADKLDEDLFIRVIIN 305
           G SFGI+L WK+ LVPVP TVTVFTV KTLEQ A  K + KWQQ++ K+ E++ IRV++ 
Sbjct: 243 GASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLR 302

Query: 306 VAANADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAG 365
            A N    G KTV+  Y   FLG+   LLKVM ++FPELGLT+KDC E SWI++ L+  G
Sbjct: 303 AAGN---DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG 362

Query: 366 YPSGTPPEVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGG 425
           +P+G+P E+LLQ KS   K+YFKA SDFVK+PIP  GL+G++KRL E  +  + W PYGG
Sbjct: 363 FPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGG 422

Query: 426 MMGKISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREA 485
           MM KI E+ IPFPHR G LFKI Y + W + D    + + WIK++YNYM PYVS  PR+A
Sbjct: 423 MMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQA 482

Query: 486 YVNYRDLDLGINK-KGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPI 537
           YVNYRDLD G NK     ++I+A  WG +YFK NF RL+++KTK+DP+NFFRHEQSIPP+
Sbjct: 483 YVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 540

BLAST of Lag0014098 vs. ExPASy TrEMBL
Match: A0A5A7UG10 (Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009760 PE=3 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 9.4e-306
Identity = 516/552 (93.48%), Postives = 533/552 (96.56%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMV  NPKYKSFV  LFLVFLLSSSS VDS+SLEESFLQCLNENSQFSVPYSSFCAPNN+
Sbjct: 1   MMVSTNPKYKSFVAPLFLVFLLSSSSWVDSSSLEESFLQCLNENSQFSVPYSSFCAPNNS 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQ+AVICSKQL+IHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSY SE ETPFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
           TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           N    GQKTVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NG---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFE+ESPLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQKG+ NQDKHL+WIKKLYNYMTPYVSQLPREAYVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQS+PPIPTSE+
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSVPPIPTSEA 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 549

BLAST of Lag0014098 vs. ExPASy TrEMBL
Match: A0A1S3AYY2 (flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC103484298 PE=3 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.6e-305
Identity = 517/552 (93.66%), Postives = 532/552 (96.38%), Query Frame = 0

Query: 1   MMVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNA 60
           MMV  NPKYKSFV  LFLVFLLSSSS VDS SLEESFLQCLNENSQFSVPYSSFCAPNN+
Sbjct: 1   MMVSTNPKYKSFVAPLFLVFLLSSSSWVDSFSLEESFLQCLNENSQFSVPYSSFCAPNNS 60

Query: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEG 120
           TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQ+AVICSKQL+IHLRVRSGGHDYEG
Sbjct: 61  TFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQSAVICSKQLRIHLRVRSGGHDYEG 120

Query: 121 LSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLC 180
           LSY SE ETPFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRIAEKSKVHGFPAGLC
Sbjct: 121 LSYASEMETPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLC 180

Query: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGG 240
           TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRIL+REAMGEDLFWAIRGGGGG
Sbjct: 181 TSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILNREAMGEDLFWAIRGGGGG 240

Query: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300
           SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA
Sbjct: 241 SFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAA 300

Query: 301 NADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPS 360
           N    GQKTVSTAYNALFLGDA+RLLKVMGE+FPELGLTRKDCIETSWIKSVLYIAGYPS
Sbjct: 301 NG---GQKTVSTAYNALFLGDASRLLKVMGENFPELGLTRKDCIETSWIKSVLYIAGYPS 360

Query: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGK 420
           GTPPEVLLQGKSTFKNYFKAKSDFVK PIPETGLEGLWKRLFE+ESPLMIWNPYGGMMGK
Sbjct: 361 GTPPEVLLQGKSTFKNYFKAKSDFVKTPIPETGLEGLWKRLFEDESPLMIWNPYGGMMGK 420

Query: 421 ISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNY 480
           ISETEIPFPHRRGVLFKIQYL+TWQKG+ NQDKHL+WIKKLYNYMTPYVSQLPREAYVNY
Sbjct: 421 ISETEIPFPHRRGVLFKIQYLTTWQKGEENQDKHLQWIKKLYNYMTPYVSQLPREAYVNY 480

Query: 481 RDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 540
           RDLDLGINK  NTSYI++IGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTSE+
Sbjct: 481 RDLDLGINKNSNTSYIESIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSEA 540

Query: 541 LRSFSSSKRGFD 553
           LRSF SSKRGFD
Sbjct: 541 LRSFGSSKRGFD 549

BLAST of Lag0014098 vs. ExPASy TrEMBL
Match: A0A6J1CYZ1 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.3e-302
Identity = 515/551 (93.47%), Postives = 526/551 (95.46%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61
           MVF  PKYKS VGSLFLVFLLSSS  VDSASLEESFLQCLNENSQFSVP+SSFCAPNNAT
Sbjct: 1   MVF--PKYKSVVGSLFLVFLLSSSCWVDSASLEESFLQCLNENSQFSVPFSSFCAPNNAT 60

Query: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 121
           FNALLQSTAQNLRYLE SVPKPLFIFTP +DSHVQAAVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 61  FNALLQSTAQNLRYLETSVPKPLFIFTPAYDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 120

Query: 122 SYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCT 181
           SY SETET F+ILDLAKLRS+KVDIEGNSAWVQAGATIGE YYRIAEKSKVHGFPAGLCT
Sbjct: 121 SYASETETSFMILDLAKLRSIKVDIEGNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 180

Query: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGS 241
           SLGVGGHITGGAYG MMRKYGLGADNVVDARIVD NGRILDREAMGED+FWAIRGGGGGS
Sbjct: 181 SLGVGGHITGGAYGPMMRKYGLGADNVVDARIVDANGRILDREAMGEDVFWAIRGGGGGS 240

Query: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301
           FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 241 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 300

Query: 302 ADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361
           ADKK  KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIE SWIKSVLYIAG+PSG
Sbjct: 301 ADKK--KTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIEMSWIKSVLYIAGFPSG 360

Query: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKI 421
           TPPEVLLQGKSTFKNYFKAKSDFVK+PI ETGLEGLWKRLFEEESPL IWNPYGGMMGKI
Sbjct: 361 TPPEVLLQGKSTFKNYFKAKSDFVKEPISETGLEGLWKRLFEEESPLTIWNPYGGMMGKI 420

Query: 422 SETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYR 481
           SETEIPFPHRRG+LFKIQYL+ WQKGD NQ+KHLEWIKKLYNYMTPYVSQLPREAYVNYR
Sbjct: 421 SETEIPFPHRRGILFKIQYLTLWQKGDSNQEKHLEWIKKLYNYMTPYVSQLPREAYVNYR 480

Query: 482 DLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSESL 541
           DLDLGINKKGNTSYI AIGWGTRYFKENFGRLLRVKTK+DPDNFFRHEQSIPPIPTS S 
Sbjct: 481 DLDLGINKKGNTSYIDAIGWGTRYFKENFGRLLRVKTKVDPDNFFRHEQSIPPIPTSASF 540

Query: 542 RSFSSSKRGFD 553
           RSF SS R FD
Sbjct: 541 RSFGSSSREFD 547

BLAST of Lag0014098 vs. ExPASy TrEMBL
Match: A0A6J1IQ34 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3 SV=1)

HSP 1 Score: 1031.2 bits (2665), Expect = 1.6e-297
Identity = 502/552 (90.94%), Postives = 530/552 (96.01%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61
           ++F NPKYKSFV SLFLVFLLSSS+ V SASL+ESFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQESFLQCLNENSQFSVPYSTFCAPNNAT 62

Query: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 121
           FN LLQS+AQNLRYLE SVPKPLFIFTPL DS+VQAAVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 63  FNTLLQSSAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122

Query: 122 SYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCT 181
           SYVS+ E+PFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRIAEKSKVHGFPAGLCT
Sbjct: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRIAEKSKVHGFPAGLCT 182

Query: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGS 241
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRILDREAMGEDLFWAIRGGGGGS
Sbjct: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242

Query: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302

Query: 302 ADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361
            DKKGQKTVSTAYNA FLG+ANRLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 303 TDKKGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362

Query: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKI 421
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+EEESPLMIWNPYGGMM KI
Sbjct: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422

Query: 422 SETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYR 481
           SET+IPFPHRRGVL+KIQYL+TWQK +GN+D+HL+WIKKLYNYM PYVSQLPREAYVNYR
Sbjct: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482

Query: 482 DLDLGINKK-GNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 541
           DLDLGIN+K  NTSYI+AIGWGTRYF++NFGRLLRVKTK+DPDNFFRHEQSIPPIPTSES
Sbjct: 483 DLDLGINEKNSNTSYIEAIGWGTRYFRQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542

Query: 542 LRSFSSSKRGFD 553
           LR F SS+RGFD
Sbjct: 543 LR-FGSSERGFD 553

BLAST of Lag0014098 vs. ExPASy TrEMBL
Match: A0A6J1ECW5 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE=3 SV=1)

HSP 1 Score: 1029.6 bits (2661), Expect = 4.7e-297
Identity = 501/552 (90.76%), Postives = 529/552 (95.83%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPYSSFCAPNNAT 61
           ++F NPKYKSFV SLFLVFLLSSS+ V SASL++SFLQCLNENSQFSVPYS+FCAPNNAT
Sbjct: 3   VLFINPKYKSFVSSLFLVFLLSSSTWVASASLQDSFLQCLNENSQFSVPYSTFCAPNNAT 62

Query: 62  FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGL 121
           FN LLQSTAQNLRYLE SVPKPLFIFTPL DS+VQAAVICSKQLKIHLRVRSGGHDYEGL
Sbjct: 63  FNTLLQSTAQNLRYLEASVPKPLFIFTPLLDSNVQAAVICSKQLKIHLRVRSGGHDYEGL 122

Query: 122 SYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCT 181
           SYVS+ E+PFIILDLAKLRSVKVDI+ NSAWVQAGATIGE YYRI EKSKVHGFPAGLCT
Sbjct: 123 SYVSQIESPFIILDLAKLRSVKVDIDSNSAWVQAGATIGEVYYRITEKSKVHGFPAGLCT 182

Query: 182 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGS 241
           SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVD NGRILDREAMGEDLFWAIRGGGGGS
Sbjct: 183 SLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDANGRILDREAMGEDLFWAIRGGGGGS 242

Query: 242 FGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAAN 301
           FGIILWWKLNLVPVPS VTVFTVAKTLEQ ATKILYKWQQVADKLDEDLFIRVIINVAAN
Sbjct: 243 FGIILWWKLNLVPVPSIVTVFTVAKTLEQDATKILYKWQQVADKLDEDLFIRVIINVAAN 302

Query: 302 ADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 361
            DK+GQKTVSTAYNA FLG+ANRLLK+MGESFPELGLTRKDCIETSWIKSVLYIAGYPSG
Sbjct: 303 TDKQGQKTVSTAYNAQFLGNANRLLKIMGESFPELGLTRKDCIETSWIKSVLYIAGYPSG 362

Query: 362 TPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKI 421
           TPPEVLLQGKSTFKN+FKAKSDFV+DPIPETGLEGLWKRL+EEESPLMIWNPYGGMM KI
Sbjct: 363 TPPEVLLQGKSTFKNFFKAKSDFVRDPIPETGLEGLWKRLYEEESPLMIWNPYGGMMAKI 422

Query: 422 SETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYR 481
           SET+IPFPHRRGVL+KIQYL+TWQK +GN+D+HL+WIKKLYNYM PYVSQLPREAYVNYR
Sbjct: 423 SETQIPFPHRRGVLYKIQYLTTWQKEEGNEDRHLQWIKKLYNYMAPYVSQLPREAYVNYR 482

Query: 482 DLDLGINKK-GNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIPTSES 541
           DLDLGIN+K  NTSYI+AIGWGTRYFK+NFGRLLRVKTK+DPDNFFRHEQSIPPIPTSES
Sbjct: 483 DLDLGINEKNSNTSYIEAIGWGTRYFKQNFGRLLRVKTKVDPDNFFRHEQSIPPIPTSES 542

Query: 542 LRSFSSSKRGFD 553
           LR F SS+RGFD
Sbjct: 543 LR-FGSSERGFD 553

BLAST of Lag0014098 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 746.1 bits (1925), Expect = 2.0e-215
Identity = 357/535 (66.73%), Postives = 442/535 (82.62%), Query Frame = 0

Query: 2   MVFANPKYKSFVGSLFLVFLLSSSSCVDSASLEESFLQCLNENSQFSVPY-SSFCAPN-N 61
           M FA  K  +   +LFLV LL  S  + S++L++ F++CL +NS  S P  +SF +P+ N
Sbjct: 1   MAFAISKRNA---TLFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQN 60

Query: 62  AT-FNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDY 121
           AT F   L+STAQNLRYL PS PKP+FIF PL+++HVQAAV+C+K+L++HLR+RSGGHDY
Sbjct: 61  ATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDY 120

Query: 122 EGLSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAG 181
           EGLS+V+E ETPF+I+DL+KLR V VD++ NSAW  AGATIGE YYRI EKS+ HGFPAG
Sbjct: 121 EGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAG 180

Query: 182 LCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGG 241
           LC+SLG+GGH+ GGAYGSMMRK+GLGADNV+DARIVD NG+ILDR AMGED+FWAIRGGG
Sbjct: 181 LCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGG 240

Query: 242 GGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINV 301
           GGSFG+IL WK+ LVPVP+TVTVFTV KTLEQ  TK+LYKW+Q+ADKLD+DLFIRVII+ 
Sbjct: 241 GGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISP 300

Query: 302 AANADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGY 361
           A+   K G +T+S +Y A FLGD+NRLL+VM +SFPELGLT+KDC E SWIKSV+YIAG+
Sbjct: 301 ASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGF 360

Query: 362 PSGTPPEVLLQGKSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMM 421
           P+   PE LL GKS FKN+FKAKSDFVK+PIP  GLEGLW+R  EE+SPL IWNPYGGMM
Sbjct: 361 PNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMM 420

Query: 422 GKISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYV 481
            +ISE+EIPFPHR G LFKIQ+LSTWQ G  ++++H++WI+++Y+YM  YVS+ PR+AYV
Sbjct: 421 SRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYV 480

Query: 482 NYRDLDLGINKKGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIP 534
           NYRDLDLG N +G T    A  WG +Y+K NF RL+++K + DPDNFFRHEQS+P
Sbjct: 481 NYRDLDLGTN-EGETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Lag0014098 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 730.7 bits (1885), Expect = 8.7e-211
Identity = 351/523 (67.11%), Postives = 422/523 (80.69%), Query Frame = 0

Query: 17  FLVFLLSSSSC---VDSASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQSTA 76
           FLV LL  S     +  +++++ F+ CL +NS  S P  SSF     N ++F   L+++A
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 77  QNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSETETP 136
           QNLRYL PS PKP FIF PL+++HVQAAV+C+K+LK+HLR+RSGGHDYEGLSYVSE ET 
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 137 FIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGGHIT 196
           F+I+DL+KLR + VDIE NSAWV AGA+IGE YYRI EKSK+HGFPAGLCTSLG+GGHI 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 197 GGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILWWKL 256
           GGAYGSMMRK+GLGADNV+DARIVD +G+IL+R AMGED+FWAIRGGGGGSFG+IL WK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 257 NLVPVPSTVTVFTVAKTLEQGATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 316
            LVPVP  VTVFTV +TLEQ  TK+LYKWQQVADKLDEDLFIRVII   +   K  ++T+
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 317 STAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 376
           ST+Y   FLGDANRLL+VM  SFP+LGLT+KDC+ETSWIKSV+YIAG+PS  P E LL G
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDG 371

Query: 377 KSTFKNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISETEIPFPH 436
           KS FKNYFKAKSD+V++PIP  GLEGLW++L EE+SPL IWNPYGGMM KI ETE PFPH
Sbjct: 372 KSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPH 431

Query: 437 RRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINKK 496
           R G LFKIQ+L+ WQ G  ++ KH+ W++++Y+YM  YVS+ PR AYVNYRDLDLG+N K
Sbjct: 432 RSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGK 491

Query: 497 GNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIP 534
           G+     A  WG RYFK NF RL+ +K K DP+NFFRHEQSIP
Sbjct: 492 GS----DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Lag0014098 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.0e-203
Identity = 343/527 (65.09%), Postives = 423/527 (80.27%), Query Frame = 0

Query: 16  LFLVFLLSSSSCVDS-ASLEESFLQCLNENSQFSVPY-SSFCAP--NNATFNALLQSTAQ 75
           L L F L + +   S ASL++ F+ C+  N+    P   +F AP  N + F+ +L+STAQ
Sbjct: 13  LVLYFSLYTITPTSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQ 72

Query: 76  NLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSETETPF 135
           NLR+L+ S+PKP FIF+P+ +SHVQA++ICSK+L++HLRVRSGGHDYEGLSYVS+ + PF
Sbjct: 73  NLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPF 132

Query: 136 IILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGGHITG 195
           I++DL+K+R V ++I+ NSAWVQ+GAT+GE YYRIAEKSKVHGFPAGLC+SLG+GGHITG
Sbjct: 133 ILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITG 192

Query: 196 GAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILWWKLN 255
           GAYGSMMRKYGLGADNV+DA+IVD NG++LDR AMGED FWAIRGG GGSFGIIL WK+ 
Sbjct: 193 GAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIK 252

Query: 256 LVPVPSTVTVFTVAKTLEQG-ATKILYKWQQVADKLDEDLFIRVIINVAANADKKGQKTV 315
           LVPVP TVTVFTV KTL+Q    KI+ KWQ+VADKL E+LFIRV+ NVA      G KTV
Sbjct: 253 LVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTG---GNKTV 312

Query: 316 STAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPPEVLLQG 375
           +T+YNALFLG    L+ VM +SFPELGLT KDCIE SW++S+ YI+G+P+ TP  VLLQG
Sbjct: 313 TTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQG 372

Query: 376 KSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISETEIPFP 435
           KS F K  FKAKSDFVK PIPE+GL+G++K+L +E+ PLMIWNPYGGMM KI E++IPFP
Sbjct: 373 KSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFP 432

Query: 436 HRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDLDLGINK 495
           HR+GVLFK+QY+++W   D    +H+ WI+ LY+YMTPYVS  PREAYVNYRDLDLG N 
Sbjct: 433 HRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNT 492

Query: 496 KGNTSYIK-AIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPI 536
           K   + IK A  WG  YFK NF RL+ +K K+DP+NFFRHEQSIPP+
Sbjct: 493 KDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of Lag0014098 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 657.1 bits (1694), Expect = 1.2e-188
Identity = 320/534 (59.93%), Postives = 404/534 (75.66%), Query Frame = 0

Query: 11  SFVGSLFLVFLLSSSSCVDSASLEESFLQCL--NENSQFSVPYSSFCAPNNAT-FNALLQ 70
           S +    L F   + +   S SL++ F++CL  N N +F++  + F    NA+ F  +L+
Sbjct: 11  SCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLE 70

Query: 71  STAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDYEGLSYVSET 130
           STAQN RYL  ++PKP FIF P+ +SHVQA+VICSK+L+IH RVRSGGHDYEG+SYVS+ 
Sbjct: 71  STAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQI 130

Query: 131 ETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAGLCTSLGVGG 190
           E PF+++DL+KLR + VDI+  SAWV+AGAT+GE YYRIAEKSK HGFPAG+  SLG+GG
Sbjct: 131 EKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGG 190

Query: 191 HITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGGGGSFGIILW 250
           HITGGAYGS+MRKYGL ADNV+DA+IVD NG++LDR +MGEDLFWAIRGG GGSFGIIL 
Sbjct: 191 HITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILS 250

Query: 251 WKLNLVPVPSTVTVFTVAKTLEQGAT-KILYKWQQVADKLDEDLFIRVIINVAANADKKG 310
           WK+ LVPVP T+TVFTV KT EQ  + KIL KWQ++AD L ++LF+RV   V+ N   K 
Sbjct: 251 WKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN---KA 310

Query: 311 QKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAGYPSGTPP-- 370
            KTV+ AY   FLG+   L++VM + FPELGLT+KDCIE SWI S++Y +G+P+  PP  
Sbjct: 311 NKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPI 370

Query: 371 EVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGGMMGKISE 430
           E+LLQ KS   K YFK KSDF K PIP  GLEG++K+L EE++ L+IW PYGG M KI E
Sbjct: 371 EILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPE 430

Query: 431 TEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREAYVNYRDL 490
           +EIPFPHR G  F IQY  +W   +   ++  +WI++LY YMTPYVS  PR+AYVNYRDL
Sbjct: 431 SEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDL 490

Query: 491 DLGINKKGNTS-YIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPIP 537
           DLG NK  + S +I+A  WG  YFK+NF RL+R+K+K+DPDNFFRHEQSIP +P
Sbjct: 491 DLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTLP 541

BLAST of Lag0014098 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 650.6 bits (1677), Expect = 1.1e-186
Identity = 313/541 (57.86%), Postives = 398/541 (73.57%), Query Frame = 0

Query: 6   NPKYKSFVGSLFLVFLLSSSSCV----DSASLEESFLQCLNENSQFSVPYSSFC---APN 65
           N K    +  + +  L  S  C+     SAS+++ F+ C+  N+  S P        A N
Sbjct: 3   NSKPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKN 62

Query: 66  NATFNALLQSTAQNLRYLEPSVPKPLFIFTPLFDSHVQAAVICSKQLKIHLRVRSGGHDY 125
            + FN +L+STAQNL++L  S+PKP FIF P+  S VQA++ICSK+L IH RVRSGGHD+
Sbjct: 63  VSLFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDF 122

Query: 126 EGLSYVSETETPFIILDLAKLRSVKVDIEGNSAWVQAGATIGEAYYRIAEKSKVHGFPAG 185
           E LSYVS  E PFI+LDL+KL+ + VDIE NSAWVQ GAT+GE YYRIAEKSK+HGFPAG
Sbjct: 123 EALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAG 182

Query: 186 LCTSLGVGGHITGGAYGSMMRKYGLGADNVVDARIVDVNGRILDREAMGEDLFWAIRGGG 245
           LCTS+G+GG++TGG YG++MRKYGL  DNV+D ++VD NG++LDR AMGEDLFWAIRGGG
Sbjct: 183 LCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGG 242

Query: 246 GGSFGIILWWKLNLVPVPSTVTVFTVAKTLEQGA-TKILYKWQQVADKLDEDLFIRVIIN 305
           G SFGI+L WK+ LVPVP TVTVFTV KTLEQ A  K + KWQQ++ K+ E++ IRV++ 
Sbjct: 243 GASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLR 302

Query: 306 VAANADKKGQKTVSTAYNALFLGDANRLLKVMGESFPELGLTRKDCIETSWIKSVLYIAG 365
            A N    G KTV+  Y   FLG+   LLKVM ++FPELGLT+KDC E SWI++ L+  G
Sbjct: 303 AAGN---DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGG 362

Query: 366 YPSGTPPEVLLQGKSTF-KNYFKAKSDFVKDPIPETGLEGLWKRLFEEESPLMIWNPYGG 425
           +P+G+P E+LLQ KS   K+YFKA SDFVK+PIP  GL+G++KRL E  +  + W PYGG
Sbjct: 363 FPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGG 422

Query: 426 MMGKISETEIPFPHRRGVLFKIQYLSTWQKGDGNQDKHLEWIKKLYNYMTPYVSQLPREA 485
           MM KI E+ IPFPHR G LFKI Y + W + D    + + WIK++YNYM PYVS  PR+A
Sbjct: 423 MMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQA 482

Query: 486 YVNYRDLDLGINK-KGNTSYIKAIGWGTRYFKENFGRLLRVKTKIDPDNFFRHEQSIPPI 537
           YVNYRDLD G NK     ++I+A  WG +YFK NF RL+++KTK+DP+NFFRHEQSIPP+
Sbjct: 483 YVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882764.10.0e+0096.01berberine bridge enzyme-like 15 [Benincasa hispida][more]
KAA0052471.11.9e-30593.48flavin-dependent oxidoreductase FOX2-like [Cucumis melo var. makuwa] >TYK13351.1... [more]
XP_008439530.13.3e-30593.66PREDICTED: flavin-dependent oxidoreductase FOX2-like [Cucumis melo][more]
XP_004134554.13.4e-30292.75berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8647767.1 hypothetical pro... [more]
XP_022146593.11.7e-30193.47berberine bridge enzyme-like 15 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O647432.8e-21466.73Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q93ZA31.2e-20967.11Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Q9FKU84.2e-20265.09Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
Q9FKU91.7e-18759.93Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=... [more]
Q9FKV01.6e-18557.86Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7UG109.4e-30693.48Flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3AYY21.6e-30593.66flavin-dependent oxidoreductase FOX2-like OS=Cucumis melo OX=3656 GN=LOC10348429... [more]
A0A6J1CYZ18.3e-30293.47berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111015758 P... [more]
A0A6J1IQ341.6e-29790.94berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111477806 PE=3... [more]
A0A6J1ECW54.7e-29790.76berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111433029 PE... [more]
Match NameE-valueIdentityDescription
AT2G34790.12.0e-21566.73FAD-binding Berberine family protein [more]
AT1G30760.18.7e-21167.11FAD-binding Berberine family protein [more]
AT5G44400.13.0e-20365.09FAD-binding Berberine family protein [more]
AT5G44390.11.2e-18859.93FAD-binding Berberine family protein [more]
AT5G44380.11.1e-18657.86FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 26..139
e-value: 6.9E-43
score: 146.8
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 143..534
e-value: 1.7E-170
score: 569.5
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 476..533
e-value: 1.7E-22
score: 79.3
NoneNo IPR availableGENE3D3.40.462.20coord: 258..472
e-value: 1.7E-170
score: 569.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 531..552
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 529..552
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 27..538
NoneNo IPR availablePANTHERPTHR32448:SF129TETRAHYDROCANNABINOLIC ACID SYNTHASE-LIKEcoord: 27..538
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 85..221
e-value: 4.8E-25
score: 87.9
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 79..255
score: 16.782116
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 56..256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014098.1Lag0014098.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding