Lag0013820 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013820
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	heat stress transcription factor B-2a-like
Location	chr1: 52991313 .. 52992966 (-)
RNA-Seq Expression	Lag0013820
Synteny	Lag0013820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAACAAGAAGAAGAAGAAGCTGGAAGTAAGAGGATTATGTCATGGAATGCAGAGGGAAATGGATTCATAGTTTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGTATGTGATCCTCTCTTTCTCTCTCTTTCTCTTTTTGGGAATGTCCCCACCATTTGTTATTCTCTGAAACATCTTGAAGAAGAGTTATTCATTATAAAGTGGATTCAATTGGCCATTTTCCTGTTTTGGGATAATGCTTCAATTCTTCCTTACGAATTGGATCTATAGGTATGTTTTGTTTATGACAGTGTGGCTACTGGTTTTTAAGGGTTGGATTGATAGAGTATGATATATTTGATCATTTCTATATGTTCTTAACATCGTTTTCCAAGTCGGGTATCAATCTAAATATTGTAATATTGGAGTCAGGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGGTCAGTGCATTGAGAAATAGCCTATGGAGTTTTGCTAATTAGAAAGATGGGTTGGTTGTAAAAGTTTCAGATAGAAAGTAATGCTTTTGGATTATCCATTTGCTCTAAGTTTGAGTTTGCATTTCCACTAATTAGTTCTAAAGAAGTTATAACAATACATTGGCTTTCTGTAAAAGTTGATGTGAAGGATTAAAGTAAGCATTTTATGCTAGTTTAATAGTCTAAATTTTATAAACTAATATAAATTCGACCTACCAAATAACGAGTTAAAACACCTAGTGCAAACAGCAAAACAGGGGAGGGAGAGTATGAATGCCTAATTAGCCACAGCTTTCTTTATGGATTATGGAAATTAGAATAGATCACACGTGATTAATTTTAAAGATACCAGAAGCTGCCAGAGGATTTCCAAGAGAGATTGGTTTGGATTGGCCTACCGATCTTAAAAGTCCGAAATGCAATACAAAATTATCTACCTTTTCTATAAAGTAAGAGATTTTGACTTTGAAAATGGAAAAGAGAAATGGCGAGCGCCATTTCTTTCTCCATAACACCTACCCCCTTCATTTTCTTTATCGAGAGAGTCATTGGCCTACCCTCTGACCTTCATTCTTTTGATCAGTTTCTCTCCAGAAGGCCGAAAATTTCAACCGTACCCTTTCCCCAAGCACAAGTTATGGATCTCAAGCTGTCCTCCAAATCGAATCCTATCAAGGACACTCATTGGCAGATCCTACTGAAGATTCTTTGTTGCAGCAACATTAA

mRNA sequence

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAACAAGAAGAAGAAGAAGCTGGAAGTAAGAGGATTATGTCATGGAATGCAGAGGGAAATGGATTCATAGTTTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGTTTCTCTCCAGAAGGCCGAAAATTTCAACCGTACCCTTTCCCCAAGCACAAGTTATGGATCTCAAGCTGTCCTCCAAATCGAATCCTATCAAGGACACTCATTGGCAGATCCTACTGAAGATTCTTTGTTGCAGCAACATTAA

Coding sequence (CDS)

ATGAACTTAGTTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTATCAAAGACTTATGATCTGTTAGAAGAAGCTGGAAGCTTCCATTTTAAAGCAGCTGATCAAGAACAAGAACAAGAACAAGAACAAGAAGAAGAAGAAGCTGGAAGTAAGAGGATTATGTCATGGAATGCAGAGGGAAATGGATTCATAGTTTGGTCTCCTGCTGAATTCTCTGAGATTTTATTGCCCAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTATGGGTTCAAGAAGACGTCGCCGAAGAGGTGGGAATTCAAGCACGAGAAGTTTCAGAGGGGAAACAGGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCGAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGCAACAATGGCGGTTGATCAGGAGAATGGCGACCATCTGTTACTAATGGAAGAGAACAAGAACCTAAGAAGACAGAAGCTGGAATTGCAGGCTCAAATAGCCCAATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGTTTCTCTCCAGAAGGCCGAAAATTTCAACCGTACCCTTTCCCCAAGCACAAGTTATGGATCTCAAGCTGTCCTCCAAATCGAATCCTATCAAGGACACTCATTGGCAGATCCTACTGAAGATTCTTTGTTGCAGCAACATTAA

Protein sequence

MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKALHIRLLDCLVSLQKAENFNRTLSPSTSYGSQAVLQIESYQGHSLADPTEDSLLQQH

Homology

[Close]

BLAST of Lag0013820 vs. NCBI nr
Match: XP_022925809.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 343.2 bits (879), Expect = 1.8e-90
Identity = 169/193 (87.56%), Postives = 184/193 (95.34%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNA 60
           M+L+MQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E E E++EE  G  RI+SWNA
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDE-EDEEDEENGGGNRIVSWNA 60

Query: 61  EGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQ 120
           +G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR+
Sbjct: 61  DGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRR 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180
           ML EIVRKKCEPSVFPA+LRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA
Sbjct: 121 MLVEIVRKKCEPSVFPAYLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180

Query: 181 QFKALHIRLLDCL 194
           QFKALHIRLLDC+
Sbjct: 181 QFKALHIRLLDCV 192

[Close]

BLAST of Lag0013820 vs. NCBI nr
Match: XP_023543806.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342.8 bits (878), Expect = 2.4e-90
Identity = 170/195 (87.18%), Postives = 183/195 (93.85%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQE--EEEAGSKRIMSW 60
           M+LVMQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E+++E E  EE  G  RI+SW
Sbjct: 1   MSLVMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDEEDEEDEEAEENGGGNRIVSW 60

Query: 61  NAEGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN 120
           NA+G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN
Sbjct: 61  NADGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGN 120

Query: 121 RQMLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQ 180
           R ML EIVRKKCEPSVFPAFLRSSHEGAATM VDQENGDHLLLMEENKNLRRQKLELQAQ
Sbjct: 121 RHMLVEIVRKKCEPSVFPAFLRSSHEGAATMGVDQENGDHLLLMEENKNLRRQKLELQAQ 180

Query: 181 IAQFKALHIRLLDCL 194
           IAQFKALHIRLLDC+
Sbjct: 181 IAQFKALHIRLLDCV 195

[Close]

BLAST of Lag0013820 vs. NCBI nr
Match: XP_022926806.1 (heat stress transcription factor B-2a-like [Cucurbita moschata])

HSP 1 Score: 337.8 bits (865), Expect = 7.6e-89
Identity = 169/189 (89.42%), Postives = 179/189 (94.71%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNG 64
           MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQE+E+E+E+EEE     RI+SWNAEGNG
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEEE----NRIVSWNAEGNG 60

Query: 65  FIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGE 124
           FIVWSPAEFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRGN+QMLGE
Sbjct: 61  FIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGE 120

Query: 125 IVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 184
           IVRKKCEPSVFPAFLRSSHEGA TMAV Q NGDHL LMEENKNLRR+KLELQA+IA+FKA
Sbjct: 121 IVRKKCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKA 180

Query: 185 LHIRLLDCL 194
           LHIRLLDCL
Sbjct: 181 LHIRLLDCL 185

[Close]

BLAST of Lag0013820 vs. NCBI nr
Match: KAG7034590.1 (Heat stress transcription factor B-2a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 336.3 bits (861), Expect = 2.2e-88
Identity = 166/189 (87.83%), Postives = 180/189 (95.24%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNG 64
           MQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E E E++EE  G  RI+SWNA+G+G
Sbjct: 1   MQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDE-EDEEDEENGGGNRIVSWNADGSG 60

Query: 65  FIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGE 124
           FIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRG+R+ML E
Sbjct: 61  FIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGHRRMLVE 120

Query: 125 IVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 184
           IVRKKCEPSVFPA+LRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA
Sbjct: 121 IVRKKCEPSVFPAYLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 180

Query: 185 LHIRLLDCL 194
           LHIRLLDC+
Sbjct: 181 LHIRLLDCV 188

[Close]

BLAST of Lag0013820 vs. NCBI nr
Match: XP_022977543.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 335.5 bits (859), Expect = 3.8e-88
Identity = 165/193 (85.49%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNA 60
           M+L+MQSS+ RNPAPFLSKTYDLL++ GSFHFKAAD+E E E E++EE+ G  RI+SWNA
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQKDGSFHFKAADEEDE-EDEEDEEDGGGNRIVSWNA 60

Query: 61  EGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQ 120
           +G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR 
Sbjct: 61  DGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRH 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180
           ML EIVRKKCEPSVFPAFLRSSHEGA TMAVDQENGDHLLLMEENKNLRRQKLELQAQIA
Sbjct: 121 MLVEIVRKKCEPSVFPAFLRSSHEGAVTMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180

Query: 181 QFKALHIRLLDCL 194
           QFKAL +RLLDC+
Sbjct: 181 QFKALRLRLLDCV 192

[Close]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.1e-29
Identity = 77/234 (32.91%), Postives = 119/234 (50.85%), Query Frame = 0


Query: 7   SSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFI 66
           SS+   P PFL+KT++L+                       E++    ++SWN +G+ FI
Sbjct: 15  SSQRSIPTPFLTKTFNLV-----------------------EDSSIDDVISWNEDGSSFI 74

Query: 67  VWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIV 126
           VW+P +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG +++L EI 
Sbjct: 75  VWNPTDFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQ 134

Query: 127 RKKCEPSVFPAFLRSSHEGAATMAVDQEN-----------------------------GD 186
           R+K   +       SS +   TM V   N                             G 
Sbjct: 135 RRKITTTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGL 194

Query: 187 HLLLMEENKNLRRQKLELQAQIAQFKALHIRLLDCLVSLQKAENFNRTLSPSTS 212
            + L+EEN+ LR Q ++L  ++ Q K++   +   + +   ++  +R+ SP  S
Sbjct: 195 SVELLEENEKLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGS 225

[Close]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot
Match: Q6VBB2 (Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2B PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-26
Identity = 73/192 (38.02%), Postives = 103/192 (53.65%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNG 64
           M+      P PFL+KTYD++                       ++AG+   +SW+A  N 
Sbjct: 42  MEGLHDAGPPPFLTKTYDMV-----------------------DDAGTDAAVSWSATSNS 101

Query: 65  FIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGE 124
           F+VW P  F+ +LLP++FKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG R +L  
Sbjct: 102 FVVWDPHAFATVLLPRFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANENFLRGQRHLLKN 161

Query: 125 IVRKK-------CEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQA 184
           I R+K        + S+ P +L   H G     +D+   D  LLM E   LR+++   +A
Sbjct: 162 IKRRKPPSHTASNQQSLGP-YLEVGHFG-YDAEIDRLKRDKQLLMAEVVKLRQEQQNTKA 205

Query: 185 QIAQFKALHIRL 190
            +   KA+  RL
Sbjct: 222 NL---KAMEDRL 205

[Close]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.9e-26
Identity = 64/139 (46.04%), Postives = 85/139 (61.15%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAE 72
           P PFL+KTY L+++       A D                  ++SWN +G+ F+VW PAE
Sbjct: 46  PTPFLTKTYQLVDD------PAVDD-----------------VISWNDDGSTFVVWRPAE 105

Query: 73  FSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEP 132
           F+  LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF ++ F+RG R++L EI R+K  P
Sbjct: 106 FARDLLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTP 160

Query: 133 SVFPAFLRSSHEGAATMAV 152
              PA   ++   A  MA+
Sbjct: 166 PA-PAATTAAVAAAIPMAL 160

[Close]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot
Match: O81821 (Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B PE=1 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 3.3e-26
Identity = 69/176 (39.20%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAEFS 74
           PFLSKTYD++                       ++  +  ++SW++  N F+VWS  EFS
Sbjct: 27  PFLSKTYDMV-----------------------DDPLTNEVVSWSSGNNSFVVWSAPEFS 86

Query: 75  EILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CE 134
           ++LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    +
Sbjct: 87  KVLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQ 146

Query: 135 PSVFPAFLRSSHEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 180
            +     ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QNQQQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.3e-26
Identity = 68/171 (39.77%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAE 72
           PAPFL KTY+++                       +++ + +I+SW+A  N FIVW+ AE
Sbjct: 21  PAPFLVKTYEMV-----------------------DDSSTDQIVSWSANNNSFIVWNHAE 80

Query: 73  FSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEP 132
           FS +LLP YFKHNNFSSFIRQLNTYGF+K  P+RWEF ++ F +  + +L  I R+K   
Sbjct: 81  FSRLLLPTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK--- 140

Query: 133 SVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFK 184
              P     SH      + DQE     +L E+   L R+K  ++A++ +FK
Sbjct: 141 ---PI---HSHSHPPASSTDQERA---VLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of Lag0013820 vs. ExPASy TrEMBL
Match: A0A6J1ED85 (heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433105 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 8.8e-91
Identity = 169/193 (87.56%), Postives = 184/193 (95.34%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNA 60
           M+L+MQSS+ RNPAPFLSKTYDLL+EAGSFHFKAAD+E E E E++EE  G  RI+SWNA
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQEAGSFHFKAADEEDE-EDEEDEENGGGNRIVSWNA 60

Query: 61  EGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQ 120
           +G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR+
Sbjct: 61  DGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRR 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180
           ML EIVRKKCEPSVFPA+LRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA
Sbjct: 121 MLVEIVRKKCEPSVFPAYLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180

Query: 181 QFKALHIRLLDCL 194
           QFKALHIRLLDC+
Sbjct: 181 QFKALHIRLLDCV 192

[Close]

BLAST of Lag0013820 vs. ExPASy TrEMBL
Match: A0A6J1EFX0 (heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC111433815 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.7e-89
Identity = 169/189 (89.42%), Postives = 179/189 (94.71%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNG 64
           MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQE+E+E+E+EEE     RI+SWNAEGNG
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEEE----NRIVSWNAEGNG 60

Query: 65  FIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGE 124
           FIVWSPAEFSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRGN+QMLGE
Sbjct: 61  FIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGE 120

Query: 125 IVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKA 184
           IVRKKCEPSVFPAFLRSSHEGA TMAV Q NGDHL LMEENKNLRR+KLELQA+IA+FKA
Sbjct: 121 IVRKKCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKA 180

Query: 185 LHIRLLDCL 194
           LHIRLLDCL
Sbjct: 181 LHIRLLDCL 185

[Close]

BLAST of Lag0013820 vs. ExPASy TrEMBL
Match: A0A6J1IQA8 (heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477844 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-88
Identity = 165/193 (85.49%), Postives = 181/193 (93.78%), Query Frame = 0


Query: 1   MNLVMQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNA 60
           M+L+MQSS+ RNPAPFLSKTYDLL++ GSFHFKAAD+E E E E++EE+ G  RI+SWNA
Sbjct: 1   MSLIMQSSRIRNPAPFLSKTYDLLQKDGSFHFKAADEEDE-EDEEDEEDGGGNRIVSWNA 60

Query: 61  EGNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQ 120
           +G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNR 
Sbjct: 61  DGSGFIVWSPADFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRH 120

Query: 121 MLGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180
           ML EIVRKKCEPSVFPAFLRSSHEGA TMAVDQENGDHLLLMEENKNLRRQKLELQAQIA
Sbjct: 121 MLVEIVRKKCEPSVFPAFLRSSHEGAVTMAVDQENGDHLLLMEENKNLRRQKLELQAQIA 180

Query: 181 QFKALHIRLLDCL 194
           QFKAL +RLLDC+
Sbjct: 181 QFKALRLRLLDCV 192

[Close]

BLAST of Lag0013820 vs. ExPASy TrEMBL
Match: A0A5A7UBZ5 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003020 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-88
Identity = 169/192 (88.02%), Postives = 178/192 (92.71%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQE---QEQEQEEEEAGSKRIMSWNAE 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQE E   +E+E+EEEE G  RI+SWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 65  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 124
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 125 LGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184
           L EIVRKKCEPSVFPAFLRSSHEG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 185 FKALHIRLLDCL 194
           FKALHIRLLDCL
Sbjct: 181 FKALHIRLLDCL 192

[Close]

BLAST of Lag0013820 vs. ExPASy TrEMBL
Match: A0A1S3AZS2 (heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC103484524 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.8e-88
Identity = 169/192 (88.02%), Postives = 178/192 (92.71%), Query Frame = 0


Query: 5   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQE---QEQEQEEEEAGSKRIMSWNAE 64
           MQSS+TRNPAPFLSKTYDLLEEAGSFHFK ADQE E   +E+E+EEEE G  RI+SWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 65  GNGFIVWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQM 124
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG R M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 125 LGEIVRKKCEPSVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQ 184
           L EIVRKKCEPSVFPAFLRSSHEG ATMAV+QENGDHLLLMEEN NLRRQKLELQAQIAQ
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 185 FKALHIRLLDCL 194
           FKALHIRLLDCL
Sbjct: 181 FKALHIRLLDCL 192

[Close]

BLAST of Lag0013820 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 129.8 bits (325), Expect = 2.9e-30
Identity = 77/234 (32.91%), Postives = 119/234 (50.85%), Query Frame = 0


Query: 7   SSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFI 66
           SS+   P PFL+KT++L+                       E++    ++SWN +G+ FI
Sbjct: 15  SSQRSIPTPFLTKTFNLV-----------------------EDSSIDDVISWNEDGSSFI 74

Query: 67  VWSPAEFSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIV 126
           VW+P +F++ LLPK+FKHNNFSSF+RQLNTYGFKK  P RWEF ++ F+RG +++L EI 
Sbjct: 75  VWNPTDFAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQ 134

Query: 127 RKKCEPSVFPAFLRSSHEGAATMAVDQEN-----------------------------GD 186
           R+K   +       SS +   TM V   N                             G 
Sbjct: 135 RRKITTTHQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGL 194

Query: 187 HLLLMEENKNLRRQKLELQAQIAQFKALHIRLLDCLVSLQKAENFNRTLSPSTS 212
            + L+EEN+ LR Q ++L  ++ Q K++   +   + +   ++  +R+ SP  S
Sbjct: 195 SVELLEENEKLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGS 225

[Close]

BLAST of Lag0013820 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-27
Identity = 68/171 (39.77%), Postives = 97/171 (56.73%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAE 72
           PAPFL KTY+++                       +++ + +I+SW+A  N FIVW+ AE
Sbjct: 21  PAPFLVKTYEMV-----------------------DDSSTDQIVSWSANNNSFIVWNHAE 80

Query: 73  FSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEP 132
           FS +LLP YFKHNNFSSFIRQLNTYGF+K  P+RWEF ++ F +  + +L  I R+K   
Sbjct: 81  FSRLLLPTYFKHNNFSSFIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRK--- 140

Query: 133 SVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFK 184
              P     SH      + DQE     +L E+   L R+K  ++A++ +FK
Sbjct: 141 ---PI---HSHSHPPASSTDQERA---VLQEQMDKLSREKAAIEAKLLKFK 156

[Close]

BLAST of Lag0013820 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-27
Identity = 69/176 (39.20%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAEFS 74
           PFLSKTYD++                       ++  +  ++SW++  N F+VWS  EFS
Sbjct: 27  PFLSKTYDMV-----------------------DDPLTNEVVSWSSGNNSFVVWSAPEFS 86

Query: 75  EILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CE 134
           ++LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    +
Sbjct: 87  KVLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQ 146

Query: 135 PSVFPAFLRSSHEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 180
            +     ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QNQQQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lag0013820 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-27
Identity = 69/176 (39.20%), Postives = 99/176 (56.25%), Query Frame = 0


Query: 15  PFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAEFS 74
           PFLSKTYD++                       ++  +  ++SW++  N F+VWS  EFS
Sbjct: 27  PFLSKTYDMV-----------------------DDPLTNEVVSWSSGNNSFVVWSAPEFS 86

Query: 75  EILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKK---CE 134
           ++LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG +Q+L  IVR+K    +
Sbjct: 87  KVLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQ 146

Query: 135 PSVFPAFLRSSHEGAATMA--------VDQENGDHLLLMEENKNLRRQKLELQAQI 180
            +     ++SS  GA            V++   D  +LM+E   LR+Q+   + Q+
Sbjct: 147 QNQQQTQVQSSSVGACVEVGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQL 179

[Close]

BLAST of Lag0013820 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-26
Identity = 80/224 (35.71%), Postives = 107/224 (47.77%), Query Frame = 0


Query: 13  PAPFLSKTYDLLEEAGSFHFKAADQEQEQEQEQEEEEAGSKRIMSWNAEGNGFIVWSPAE 72
           P PFLSKTYD++                       E+  +  I+SW+   N FIVW P E
Sbjct: 50  PPPFLSKTYDMV-----------------------EDPATDAIVSWSPTNNSFIVWDPPE 109

Query: 73  FSEILLPKYFKHNNFSSFIRQLNTYGFKKTSPKRWEFKHEKFQRGNRQMLGEIVRKKCEP 132
           FS  LLPKYFKHNNFSSF+RQLNTYGF+K  P RWEF +E F RG + +L +I R+K   
Sbjct: 110 FSRDLLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQ 169

Query: 133 SVFPAFLRSSHEGAATMAVDQENGDHLLLMEENKNLRRQKLELQAQIAQFKALHIRLLDC 192
                   SS     +  + Q  G    L   +  +   K  L+ ++ Q K     L+  
Sbjct: 170 G-----HGSSSSNPQSQQLSQGQGSMAAL---SSCVEVGKFGLEEEVEQLKRDKNVLMQE 229

Query: 193 LVSL----QKAENFNRTLSPSTSYGSQAVLQIESYQGHSLADPT 233
           LV L    Q  +N  + L        Q   QI S+   ++ +PT
Sbjct: 230 LVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIMSFLAKAVQNPT 242

The following BLAST results are available for this feature:

BLAST of Lag0013820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022925809.1	1.8e-90	87.56	heat stress transcription factor B-2a-like isoform X1 [Cucurbita moschata]	[more]
XP_023543806.1	2.4e-90	87.18	heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pep...	[more]
XP_022926806.1	7.6e-89	89.42	heat stress transcription factor B-2a-like [Cucurbita moschata]	[more]
KAG7034590.1	2.2e-88	87.83	Heat stress transcription factor B-2a, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022977543.1	3.8e-88	85.49	heat stress transcription factor B-2a-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lag0013820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SCW4	4.1e-29	32.91	Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ...	[more]
Q6VBB2	1.5e-26	38.02	Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q6Z9C8	1.9e-26	46.04	Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
O81821	3.3e-26	39.20	Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B ...	[more]
Q94BZ5	3.3e-26	39.77	Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE...	[more]

BLAST of Lag0013820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ED85	8.8e-91	87.56	heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1EFX0	3.7e-89	89.42	heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1IQA8	1.8e-88	85.49	heat stress transcription factor B-2a-like isoform X1 OS=Cucurbita maxima OX=366...	[more]
A0A5A7UBZ5	1.8e-88	88.02	Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3AZS2	1.8e-88	88.02	heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC1034845...	[more]

BLAST of Lag0013820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62020.1	2.9e-30	32.91	heat shock transcription factor B2A	[more]
AT4G13980.1	2.3e-27	39.77	winged-helix DNA-binding transcription factor family protein	[more]
AT5G16820.1	2.3e-27	39.20	heat shock factor 3	[more]
AT5G16820.2	2.3e-27	39.20	heat shock factor 3	[more]
AT4G17750.1	1.2e-26	35.71	heat shock factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 158..185
None	No IPR available	PANTHER	PTHR10015:SF308	HEAT STRESS TRANSCRIPTION FACTOR B-2A-LIKE	coord: 6..201
IPR000232	Heat shock factor (HSF)-type, DNA-binding	SMART	SM00415	hsfneu3	coord: 12..128 e-value: 4.9E-35 score: 132.4
IPR000232	Heat shock factor (HSF)-type, DNA-binding	PFAM	PF00447	HSF_DNA-bind	coord: 16..128 e-value: 5.7E-27 score: 94.2
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 8..129 e-value: 2.1E-29 score: 104.0
IPR027725	Heat shock transcription factor family	PANTHER	PTHR10015	HEAT SHOCK TRANSCRIPTION FACTOR	coord: 6..201
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 52..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013820.1	Lag0013820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding