Lag0013722 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013722
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr1: 52275564 .. 52277384 (-)
RNA-Seq Expression	Lag0013722
Synteny	Lag0013722

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGCCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGTAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATCATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCCTGAGCAAATCCTTAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACATCCCTCGCGGGGAAGCGGCTCTGGCTGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTGGGGATTTTGGTTTCGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGGTATCCGAATTGGTTGAAGAGCGGAGATCGGAGGCTGTTGGGGCTTATGGGGAGAGAAATGGAAGCGAATATAGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAGGCGATTCCAGACAAGAGTAAGAGTAGAATTGTTATATATGTGAAGAAGGGGATTTATGAAGAGAATGTGGAAATTGGGAAGAAGAAGAAGAATGTGATGATCATCGGCGATGGAATGGATTCCACCATTATCACTGCTAGCCTCAACGTTGTTGATGGATCAACTACTTTTAAATCTGCAACTGTTGGTAAAGCTCCTAATTTTGGATTGCTTTACATACAAGTCTTGTGAGAATAGTCCAAATACAATCTATTTTTAATCGTTAAAAGAAGTCGGTAAGAAGCAAGAAAAGAAACGACATAACAGTAGGTAACATTTTCTAACGTGGGTATTTGATTTGATGACGCAGCTGCCGTGGGAGATGGATTTATAGCCCAAGACATCGGCTTCAGAAACACCGCCGGGCCGGAAAAGCACCAAGCGGTGGCGCTTCGGGTCGGAGCAGACCAAGCCGTCATAAACCGGTGTCGTATCGACGCTTATCAAGACACTCTCTACACACATTCCAACCGCCAGTTCTACCGAGACTGCACCATCACCGGCACCGTGGACTTCATTTTCGGCAACGCCGCCGTTGTACTACAGAACTGCAAAATCGAACCCCGACGGCCCATGAGCAACCAGGCCAACATGGTGACGGCCCAAAGCCGAATCGACCCAAATCAGAACACGGGAACTTCAATCCAGCAATGCCAAATCGTCGCAAGCTCCGACCTCGAGCCCGTTAAGCGGTCCATTAAGACTTATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCGTTATGCAGTCCAAAATTGGCGGTCTTATAGATCCAGCGGGCTGGGCCGAGTGGAACGGAGACTTTGCATTGAAGACTCTGTATTACGGTGAGTATTCGAATAATGGGCCGGGCTCTGATGTTAGCAAACGAGTAAAATGGCCTGGTTACCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTCACAGTGGACTCGCTTATACAAGGAGGCGACTGGCTGGGCGCCTCAGGCGTGACTTTTATCCCAGGGCTGTGA

mRNA sequence

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGCCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGTAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATCATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCCTGAGCAAATCCTTAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACATCCCTCGCGGGGAAGCGGCTCTGGCTGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTGGGGATTTTGGTTTCGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGGTATCCGAATTGGTTGAAGAGCGGAGATCGGAGGCTGTTGGGGCTTATGGGGAGAGAAATGGAAGCGAATATAGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAGGCGATTCCAGACAAGAGTAAGAGTAGAATTGTTATATATGTGAAGAAGGGGATTTATGAAGAGAATGTGGAAATTGGGAAGAAGAAGAAGAATGTGATGATCATCGGCGATGGAATGGATTCCACCATTATCACTGCTAGCCTCAACGTTGTTGATGGATCAACTACTTTTAAATCTGCAACTGTTGCTGCCGTGGGAGATGGATTTATAGCCCAAGACATCGGCTTCAGAAACACCGCCGGGCCGGAAAAGCACCAAGCGGTGGCGCTTCGGGTCGGAGCAGACCAAGCCGTCATAAACCGGTGTCGTATCGACGCTTATCAAGACACTCTCTACACACATTCCAACCGCCAGTTCTACCGAGACTGCACCATCACCGGCACCGTGGACTTCATTTTCGGCAACGCCGCCGTTGTACTACAGAACTGCAAAATCGAACCCCGACGGCCCATGAGCAACCAGGCCAACATGGTGACGGCCCAAAGCCGAATCGACCCAAATCAGAACACGGGAACTTCAATCCAGCAATGCCAAATCGTCGCAAGCTCCGACCTCGAGCCCGTTAAGCGGTCCATTAAGACTTATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCGTTATGCAGTCCAAAATTGGCGGTCTTATAGATCCAGCGGGCTGGGCCGAGTGGAACGGAGACTTTGCATTGAAGACTCTGTATTACGGTGAGTATTCGAATAATGGGCCGGGCTCTGATGTTAGCAAACGAGTAAAATGGCCTGGTTACCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTCACAGTGGACTCGCTTATACAAGGAGGCGACTGGCTGGGCGCCTCAGGCGTGACTTTTATCCCAGGGCTGTGA

Coding sequence (CDS)

ATGGCGATGACGACCCATCAGCCTCTTCTTCAACCCCCCAAAACTACCCGCTCCAAAACCTTCTCCACAATCCTCTCTTTTTCTGCAATTTTCTGCTCAGCCATTTTCATTACCAACAAATTGATCGAATCAAACCCATCTCAAACTGTAAATGATCAGCCCTTCCTTCACACCATCTGCCACAAGGCTTACAACCCATCATCCTGCATTGAAATGGCCGCCTCAGAGTTCCCCTTACACCAAGAAACGAAAACGAAGAACGGGGCTCATTTCTTACAATTTTTCCTGAGCAAATCCTTAGCGAAAGTCATTAACACAATCGAGAGCGCCAGAGACCTCAGTCGTAGAATCAACATCCCTCGCGGGGAAGCGGCTCTGGCTGATTGCGTCGAGCTCATGGAGATCTCGATGGACCGAATAGCGGACTCGATTTCGGCTCTGAAAAACAGAACGAGCGGCTCGATCGAAAACGCGCACACATGGCTGAGCAGCGTGCTGACGAACCACATGACGTGCTCGGATGAGGTCGAGAATTCAATTCCGAGAGCGGCGGGCATGGATCTGGGGCTTGAAGAGCTGATTCTGAGAGGGAGAAATTCACTGGGGATTTTGGTTTCGATTTGGGGATCGGAGATGGAAGAGAATGGGAATGGGTATCCGAATTGGTTGAAGAGCGGAGATCGGAGGCTGTTGGGGCTTATGGGGAGAGAAATGGAAGCGAATATAGTGGTGGCGAAGGATGGGAGTGGGAATTTCAAGACGGTGAAGGAGGCGGTTGAGGCGATTCCAGACAAGAGTAAGAGTAGAATTGTTATATATGTGAAGAAGGGGATTTATGAAGAGAATGTGGAAATTGGGAAGAAGAAGAAGAATGTGATGATCATCGGCGATGGAATGGATTCCACCATTATCACTGCTAGCCTCAACGTTGTTGATGGATCAACTACTTTTAAATCTGCAACTGTTGCTGCCGTGGGAGATGGATTTATAGCCCAAGACATCGGCTTCAGAAACACCGCCGGGCCGGAAAAGCACCAAGCGGTGGCGCTTCGGGTCGGAGCAGACCAAGCCGTCATAAACCGGTGTCGTATCGACGCTTATCAAGACACTCTCTACACACATTCCAACCGCCAGTTCTACCGAGACTGCACCATCACCGGCACCGTGGACTTCATTTTCGGCAACGCCGCCGTTGTACTACAGAACTGCAAAATCGAACCCCGACGGCCCATGAGCAACCAGGCCAACATGGTGACGGCCCAAAGCCGAATCGACCCAAATCAGAACACGGGAACTTCAATCCAGCAATGCCAAATCGTCGCAAGCTCCGACCTCGAGCCCGTTAAGCGGTCCATTAAGACTTATTTGGGCCGCCCATGGAAGGAGTATTCGAGGACCGTCGTTATGCAGTCCAAAATTGGCGGTCTTATAGATCCAGCGGGCTGGGCCGAGTGGAACGGAGACTTTGCATTGAAGACTCTGTATTACGGTGAGTATTCGAATAATGGGCCGGGCTCTGATGTTAGCAAACGAGTAAAATGGCCTGGTTACCATGTCATTACGAGCCCATCTGAGGCCCAAAAGTTCACAGTGGACTCGCTTATACAAGGAGGCGACTGGCTGGGCGCCTCAGGCGTGACTTTTATCCCAGGGCTGTGA

Protein sequence

MAMTTHQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICHKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRAAGMDLGLEELILRGRNSLGILVSIWGSEMEENGNGYPNWLKSGDRRLLGLMGREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWLGASGVTFIPGL

Homology

[Close]

BLAST of Lag0013722 vs. NCBI nr
Match: XP_038881670.1 (pectinesterase [Benincasa hispida])

HSP 1 Score: 934.5 bits (2414), Expect = 4.2e-268
Identity = 468/556 (84.17%), Postives = 502/556 (90.29%), Query Frame = 0


Query: 3   MTTHQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICHK 62
           M THQPLLQPPKT+RSKT  TIL F+A+ CSAIFITNK I+SN S+  ND  FLH +CHK
Sbjct: 1   MATHQPLLQPPKTSRSKTIPTILCFAAVLCSAIFITNKFIKSNSSE--NDLSFLHNLCHK 60

Query: 63  AYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRG 122
           AYNPSSCIEMAASEFP  +E KT N  HFLQ FL KS+ KVINTI+ A+DLS+RIN PRG
Sbjct: 61  AYNPSSCIEMAASEFPFPREIKTTNRGHFLQSFLRKSIPKVINTIDRAKDLSQRINNPRG 120

Query: 123 EAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPR 182
           EAALADCVELMEIS  RI DS+SALKNRTSGSIENAHTWLSSVLTNH+TC DEVENS+ R
Sbjct: 121 EAALADCVELMEISNSRIIDSVSALKNRTSGSIENAHTWLSSVLTNHITCWDEVENSVSR 180

Query: 183 AAGMDLGLEELILRGRNSLGILVSIWGSEMEENG------NGYPNWLKSGDRRLLGLMGR 242
           AA MDLGLEELI+RGRNSLG+ VSIW SE+E  G      NGYP+WLK GDRRLLG+ G+
Sbjct: 181 AAAMDLGLEELIMRGRNSLGMFVSIWESEIENMGELEKRVNGYPSWLKKGDRRLLGVPGK 240

Query: 243 EMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIG 302
           EMEANIVVAKDGSGNF TV EAVE++PDKSK+RIVIYVKKGIY+E VE+GKKKKNVMI+G
Sbjct: 241 EMEANIVVAKDGSGNFMTVTEAVESVPDKSKNRIVIYVKKGIYKEKVEVGKKKKNVMIVG 300

Query: 303 DGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQ 362
           DGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGPEKHQAVALRVGADQ
Sbjct: 301 DGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGADQ 360

Query: 363 AVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 422
           +VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN
Sbjct: 361 SVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQAN 420

Query: 423 MVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGL 482
           MVTAQ R+DPNQNTG+SIQ+C+IVASSDLEPVK+SIKTYLGRPWKEYSRTVVMQS IG L
Sbjct: 421 MVTAQGRMDPNQNTGSSIQKCEIVASSDLEPVKQSIKTYLGRPWKEYSRTVVMQSTIGDL 480

Query: 483 IDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQ 542
           I PAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKW GYHVITSPSEAQKFTVDSLIQ
Sbjct: 481 IQPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWAGYHVITSPSEAQKFTVDSLIQ 540

Query: 543 GGDWLGASGVTFIPGL 553
           GG+WLG SG TFIPGL
Sbjct: 541 GGEWLGPSGATFIPGL 554

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BLAST of Lag0013722 vs. NCBI nr
Match: XP_004134752.1 (pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cucumis sativus])

HSP 1 Score: 921.0 bits (2379), Expect = 4.8e-264
Identity = 462/558 (82.80%), Postives = 501/558 (89.78%), Query Frame = 0


Query: 3   MTTHQP--LLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTIC 62
           M THQP  LLQPPKT+RSKT  T+L F+A+ CSAIFITNK I+ NPS+  ND PFL  IC
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSE--NDLPFLQNIC 60

Query: 63  HKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIP 122
           HKAY+PSSCIEMAASEFPL    KT N   FLQ FL KS+ KVI+TIE A+D+ +RIN P
Sbjct: 61  HKAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGEAALADC+ELMEIS  RI DS+ ALKNRTSGSIEN+HTWLSSVLTNH+TC DEVE+S+
Sbjct: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRRLLGLM 242
            RAA MDLGLEELI+RGRNSLG+LVSIWG       E+E+ GNGYP+WLK GDRRLLG++
Sbjct: 181 SRAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVL 240

Query: 243 GREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 302
           GREME NIVVAKDGSGNFKTVKEAVE++PDKSK+RIVIYVK+G YEENVE+GKKKKNVMI
Sbjct: 241 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 300

Query: 303 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 362
           +GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGPEKHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360

Query: 363 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPM+NQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420

Query: 423 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 482
           ANMVTAQ RIDPNQNTGTSIQQC IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS+IG
Sbjct: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 480

Query: 483 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 542
            LI PAGWAEWNGDFALKTLYYGEYSN GPGSDVSKRVKW GYH+ITSPSEAQKFTVDSL
Sbjct: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSL 540

Query: 543 IQGGDWLGASGVTFIPGL 553
           IQGG+WLG SG TFIPGL
Sbjct: 541 IQGGEWLGPSGATFIPGL 555

[Close]

BLAST of Lag0013722 vs. NCBI nr
Match: XP_023518219.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 909.8 bits (2350), Expect = 1.1e-260
Identity = 457/551 (82.94%), Postives = 499/551 (90.56%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICH 62
           M TH+PLL QPPKTTR KTF TIL FS + CSAIFITNKLI S+PS+     PFLH IC+
Sbjct: 35  MATHEPLLPQPPKTTRPKTFFTILFFSLLLCSAIFITNKLINSDPSEN-GHHPFLHNICN 94

Query: 63  KAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPR 122
            AYN SSCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIE+A+DL RRIN PR
Sbjct: 95  MAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIENAKDLRRRINNPR 154

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI ALK+R  G+IENAHTWLSSVLTNH+T S+ +ENSI 
Sbjct: 155 GEAALADCVELMEISMDRITDSILALKSRRCGTIENAHTWLSSVLTNHVTYSEGIENSIS 214

Query: 183 RAAGMDLGLEELILRGRNSLGILVSIWGSEMEENGNGYPNWLKSGDRRLLGLMGREMEAN 242
           + A MDLG+EELI+RGRNSLG+LVSIWGS  EE   GYP+WLK GDRRLL ++GR M+A+
Sbjct: 215 KTAAMDLGVEELIVRGRNSLGMLVSIWGSGKEE--MGYPSWLKKGDRRLLEVVGRGMKAD 274

Query: 243 IVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDS 302
           IVVAKDGSGNFKTVKEAVEA+PDKSK+RIVIYVKKGIY+ENVE+GK KKNVM++GDGMDS
Sbjct: 275 IVVAKDGSGNFKTVKEAVEAVPDKSKNRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDS 334

Query: 303 TIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINR 362
           TIITASLNVVDGSTTF+SATVAAVGDGFIAQDI FRNTAGPEKHQAVALRVGADQ+VINR
Sbjct: 335 TIITASLNVVDGSTTFRSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGADQSVINR 394

Query: 363 CRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 422
           CRIDAYQDTLY H+NRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ
Sbjct: 395 CRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 454

Query: 423 SRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAG 482
            R DPNQNTGTSIQ+CQIVASSDLEPVK+SIKTYLGRPWKEYSRTVVMQS +G LIDPAG
Sbjct: 455 GRTDPNQNTGTSIQKCQIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAG 514

Query: 483 WAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWL 542
           WAEWNGDFALKTLYYGEYSNNGPGS+VSKRVKWPGYHVITSP+ AQKFTVDSLIQG +WL
Sbjct: 515 WAEWNGDFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWL 574

Query: 543 GASGVTFIPGL 553
           G+SGVTFIPGL
Sbjct: 575 GSSGVTFIPGL 582

[Close]

BLAST of Lag0013722 vs. NCBI nr
Match: TYK13062.1 (pectinesterase [Cucumis melo var. makuwa])

HSP 1 Score: 909.8 bits (2350), Expect = 1.1e-260
Identity = 458/558 (82.08%), Postives = 495/558 (88.71%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CSAIF+TNK I+ NP +  ND PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYE--NDLPFLHNIC 60

Query: 63  HKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIP 122
           HKAYNPSSCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RIN P
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSGSIE++HTWLSSVLTNH+TC DEVE S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRRLLGLM 242
            RAA MDLGLEELI+RGRNSLG+LVSIWG       E+ + GNGYP+WLK GDRRLLG++
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVL 240

Query: 243 GREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 302
           GREME NIVVAKDGSGNFKTV+EAVE++PDKSK RIVIYVKKG Y+ENVE+GKKKKNVMI
Sbjct: 241 GREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMI 300

Query: 303 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 362
           +GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGP KHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGA 360

Query: 363 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPM+NQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420

Query: 423 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 482
           ANMVTAQ RIDPNQNTGTSIQQC IVASSDLEPVK+SIKTYLGRPWKEYSRTVVMQS+IG
Sbjct: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIG 480

Query: 483 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 542
            LI PAGWAEW+GDFALKTLYYGEYSN GPGSDVSKRVKW GYHVITSPSEAQKFTV+SL
Sbjct: 481 DLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSL 540

Query: 543 IQGGDWLGASGVTFIPGL 553
           IQGG+WLG SG +FIPGL
Sbjct: 541 IQGGEWLGPSGASFIPGL 555

[Close]

BLAST of Lag0013722 vs. NCBI nr
Match: XP_023002934.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 908.3 bits (2346), Expect = 3.3e-260
Identity = 457/551 (82.94%), Postives = 499/551 (90.56%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICH 62
           M TH+PLL QPP+TTR KTF TIL FS +  SAIFITNKLI SNPS+  +  PFLH IC+
Sbjct: 1   MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSEN-DHHPFLHNICY 60

Query: 63  KAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPR 122
           KAYN SSCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIESA+DL RRIN PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI AL+ R  G+IENAHTWLSSVLTNH+ CSDE+ENSI 
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 183 RAAGMDLGLEELILRGRNSLGILVSIWGSEMEENGNGYPNWLKSGDRRLLGLMGREMEAN 242
           +AA MDLG+EELI+ GRNSLG+LVSIWGS  +E G GYP+WLK GDRRLL ++GR M+A+
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE-GMGYPSWLKKGDRRLLEVVGRGMKAD 240

Query: 243 IVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDS 302
           IVVAKDGSGNFKTVKEAVEA+PDKSKSRIVIYVKKGIY+ENVE+GK KKNVM++GDGMDS
Sbjct: 241 IVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDS 300

Query: 303 TIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINR 362
           TIITASLNVVDGSTTFKSATVAAVGDGFIAQDI FRNTA PEKHQAVALRVGADQ+VINR
Sbjct: 301 TIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINR 360

Query: 363 CRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 422
           CRIDAYQDTLY H+NRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ
Sbjct: 361 CRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 420

Query: 423 SRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAG 482
            R DPNQNTGTSIQ+C+IVAS DLEPVK+SIKTYLGRPWKEYSRTVVMQS +G LIDPAG
Sbjct: 421 GRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAG 480

Query: 483 WAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWL 542
           WAEWNG+FALKTLYYGEYSNNGPGS+VSKRVKWPGYHVITSP+ AQKFTVDSLIQG +WL
Sbjct: 481 WAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWL 540

Query: 543 GASGVTFIPGL 553
           G+SGVTFIPGL
Sbjct: 541 GSSGVTFIPGL 549

[Close]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot
Match: P83947 (Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=204231 PE=1 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 7.2e-170
Identity = 315/560 (56.25%), Postives = 400/560 (71.43%), Query Frame = 0


Query: 3   MTTHQP-LLQPPKTTRSKT--FSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTI 62
           M  +QP LL+  K+  SK   F  ++SF+A+  +          S+P  +++     H I
Sbjct: 1   MEINQPNLLEASKSCYSKITFFLLVISFAALVSTGF--------SSPELSLH-----HKI 60

Query: 63  CHKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINI 122
           C ++ N  SC+ M +    L+      +  + L+ FL K+  ++    E+A D SRRIN 
Sbjct: 61  CDQSVNKESCLAMISEVTGLNM----ADHRNLLKSFLEKTTPRIQKAFETANDASRRINN 120

Query: 123 PRGEAALADCVELMEISMDRIADSISAL--KNRTSGSIENAHTWLSSVLTNHMTCSDEVE 182
           P+   AL DC ELM++S +R+ DSIS L  +N T+ S E+ H WLS VLTNH+TC D +E
Sbjct: 121 PQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHEDLHVWLSGVLTNHVTCLDGLE 180

Query: 183 NSIPR--AAGMDLGLEELILRGRNSLGILVSIWGSE---MEENGNGYPNWLKSGDRRLLG 242
                     M+  L ELILR R SL I V+++ ++   +E     +P W+ +GDRRLL 
Sbjct: 181 EGSTDYIKTLMESHLNELILRARTSLAIFVTLFPAKSNVIEPVTGNFPTWVTAGDRRLLQ 240

Query: 243 LMGREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNV 302
            +G+++E +IVVAKDGSG+++T+ EAV AIPD SK R+++ V+ GIYEENV+ G +KKNV
Sbjct: 241 TLGKDIEPDIVVAKDGSGDYETLNEAVAAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNV 300

Query: 303 MIIGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRV 362
           M++G+GMD TIIT S NVVDGSTTF SATVAAVGDGFIAQDI F+NTAGPEK+QAVALR+
Sbjct: 301 MLVGEGMDYTIITGSRNVVDGSTTFDSATVAAVGDGFIAQDICFQNTAGPEKYQAVALRI 360

Query: 363 GADQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMS 422
           GAD+ VINRCRIDAYQDTLY H+ RQFYRD  ITGTVDFIFGNAAVV QNC + PR+ M 
Sbjct: 361 GADETVINRCRIDAYQDTLYPHNYRQFYRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMK 420

Query: 423 NQANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSK 482
            Q N +TAQ R DPNQNTGTSIQ C+I AS+DLEPV+ + K+YLGRPWKEYSRTVVM+S 
Sbjct: 421 GQENTITAQGRTDPNQNTGTSIQNCEIFASADLEPVEDTFKSYLGRPWKEYSRTVVMESY 480

Query: 483 IGGLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVD 542
           I  +IDPAGW EW+ DFALKTL+YGEY N GPGS  S+RVKWPGYHVITSP  A++FTV 
Sbjct: 481 ISDVIDPAGWLEWDRDFALKTLFYGEYRNGGPGSGTSERVKWPGYHVITSPEVAEQFTVA 540

Query: 543 SLIQGGDWLGASGVTFIPGL 553
            LIQGG WLG++GV +  GL
Sbjct: 541 ELIQGGSWLGSTGVDYTAGL 543

[Close]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot
Match: P09607 (Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2)

HSP 1 Score: 568.2 bits (1463), Expect = 1.0e-160
Identity = 298/552 (53.99%), Postives = 378/552 (68.48%), Query Frame = 0


Query: 4   TTHQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICHKA 63
           T  QPLL       S     IL+F      A+F+   L+     +  +       +C  A
Sbjct: 3   TPQQPLLTKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIKHS-----NLCKTA 62

Query: 64  YNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRGE 123
            +   C+   +        T   +G   L+ FL  S+ ++ N I   R +  +IN  R +
Sbjct: 63  QDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQINDIREQ 122

Query: 124 AALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRA 183
            AL DC+EL+++S+D + DSI+A+  R+     NA +WLS VLTNH+TC DE++ S  +A
Sbjct: 123 GALTDCLELLDLSVDLVCDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKA 182

Query: 184 AGMDLGLEELILRGRNSLGILVSIW--GSEMEENGNG-YPNWLKSGDRRLLGLMGREMEA 243
                 L+ELI R + +L +L S+     E+   G G  P+W+ S DR+L+   G+++ A
Sbjct: 183 MINGTNLDELISRAKVALAMLASVTTPNDEVLRPGLGKMPSWVSSRDRKLMESSGKDIGA 242

Query: 244 NIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMD 303
           N VVAKDG+G ++T+ EAV A PDKSK+R VIYVK+G Y+ENVE+  +K N+MIIGDGM 
Sbjct: 243 NAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVSSRKMNLMIIGDGMY 302

Query: 304 STIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVIN 363
           +TIIT SLNVVDGSTTF SAT+AAVG GFI QDI  +NTAGP KHQAVALRVGAD++VIN
Sbjct: 303 ATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVIN 362

Query: 364 RCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTA 423
           RCRIDAYQDTLY HS RQFYRD  +TGT+DFIFGNAAVV Q C++  R+P   Q NMVTA
Sbjct: 363 RCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCQLVARKPGKYQQNMVTA 422

Query: 424 QSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPA 483
           Q R DPNQ TGTSIQ C I+AS DL+PV +   TYLGRPWK+YSRTVVM+S +GGLIDP+
Sbjct: 423 QGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYLGRPWKKYSRTVVMESYLGGLIDPS 482

Query: 484 GWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDW 543
           GWAEW+GDFALKTLYYGE+ NNGPG+  SKRVKWPGYHVIT P+EA  FTV  LIQGG W
Sbjct: 483 GWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAEAMSFTVAKLIQGGSW 542

Query: 544 LGASGVTFIPGL 553
           L ++ V ++ GL
Sbjct: 543 LRSTDVAYVDGL 548

[Close]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot
Match: Q96575 (Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.3e-160
Identity = 298/552 (53.99%), Postives = 376/552 (68.12%), Query Frame = 0


Query: 4   TTHQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICHKA 63
           T  QPLL       S     IL+F      A+F+   L+     +  +       +C  A
Sbjct: 3   TPQQPLLTKTHKQNSIISFKILTFVVTLFVALFLVVFLVAPYQFEIKHS-----NLCKTA 62

Query: 64  YNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRGE 123
            +   C+   +        T   +G   L  FL   + ++ N I     +  +IN  R E
Sbjct: 63  QDSQLCLSYVSDLMSNEIVTTDSDGLSILMKFLVNYVHQMNNAIPVVSKMKNQINDIRQE 122

Query: 124 AALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRA 183
            AL DC+EL++ S+D ++DSI+A+  RT     NA +WLS VLTNH+TC DE++ S  +A
Sbjct: 123 GALTDCLELLDQSVDLVSDSIAAIDKRTHSEHANAQSWLSGVLTNHVTCLDELD-SFTKA 182

Query: 184 AGMDLGLEELILRGRNSLGILVSIW--GSEMEENGNG-YPNWLKSGDRRLLGLMGREMEA 243
                 L+ELI R + +L +L S+     ++   G G  P+W+ S DR+L+   G+++ A
Sbjct: 183 MINGTNLDELISRAKVALAMLASVTTPNDDVLRPGLGKMPSWVSSRDRKLMESSGKDIGA 242

Query: 244 NIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMD 303
           N VVAKDG+G ++T+ EAV A PDKSK+R VIYVK+GIY+ENVE+  +K  +MI+GDGM 
Sbjct: 243 NAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIYKENVEVSSRKMKLMIVGDGMH 302

Query: 304 STIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVIN 363
           +TIIT +LNVVDGSTTF SAT+AAVG GFI QDI  +NTAGP KHQAVALRVGAD++VIN
Sbjct: 303 ATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNTAGPAKHQAVALRVGADKSVIN 362

Query: 364 RCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTA 423
           RCRIDAYQDTLY HS RQFYRD  +TGT+DFIFGNAAVV Q CK+  R+P   Q NMVTA
Sbjct: 363 RCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVVFQKCKLVARKPGKYQQNMVTA 422

Query: 424 QSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPA 483
           Q R DPNQ TGTSIQ C I+ASSDLEPV +   TYLGRPWK+YSRTVVM+S +GGLI+PA
Sbjct: 423 QGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKKYSRTVVMESYLGGLINPA 482

Query: 484 GWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDW 543
           GWAEW+GDFALKTLYYGE+ NNGPG+  SKRVKWPGYH IT P+EA  FTV  LIQGG W
Sbjct: 483 GWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHCITDPAEAMPFTVAKLIQGGSW 542

Query: 544 LGASGVTFIPGL 553
           L ++GV ++ GL
Sbjct: 543 LRSTGVAYVDGL 548

[Close]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 562.8 bits (1449), Expect = 4.4e-159
Identity = 306/554 (55.23%), Postives = 382/554 (68.95%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHT--ICHK 64
           ++QPLL  PK+ + K    +LSF AI  S  F T +LI  N +   ND   L T  ICH 
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNN--NDDSLLTTSQICHG 63

Query: 65  AYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRG 124
           A++  SC +   SEF     +K  N    L  FL  S+ ++ +T+    +   R N  R 
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRD 123

Query: 125 EAALADCVELMEISMDRIADSISALK--NRTSGSIENAHTWLSSVLTNHMTCSDEVEN-S 184
           +A  ADC E+M++S DR+  S+  L+  N    S  N HTWLSSVLTN+MTC + + + S
Sbjct: 124 KAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS 183

Query: 185 IPRAAGMDLGLEELILRGRNSLGILVSIWGSEMEEN---GNGYPNWLKSGDRRLLGLMGR 244
           +     +   LE+L+ R R +L I VS+  +  +      N +P+WL + DR+LL    +
Sbjct: 184 VNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPK 243

Query: 245 EME--ANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 304
            ++  AN+VVAKDG+G FKTV EAV A P+ S +R VIYVKKG+Y+E ++IGKKKKN+M+
Sbjct: 244 TLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLML 303

Query: 305 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 364
           +GDG D+TIIT SLNV+DGSTTF+SATVAA GDGF+AQDI F+NTAGP KHQAVALRV A
Sbjct: 304 VGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSA 363

Query: 365 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 424
           DQ VINRCRIDAYQDTLYTH+ RQFYRD  ITGTVDFIFGN+AVV QNC I  R P + Q
Sbjct: 364 DQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQ 423

Query: 425 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 484
            NM+TAQ R D NQNT  SIQ+C+I ASSDL PVK S+KT+LGRPWK YSRTV+MQS I 
Sbjct: 424 KNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFID 483

Query: 485 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 544
             IDPAGW  W+G+FAL TLYYGEY+N GPG+D SKRV W G+ VI    EA++FTV  L
Sbjct: 484 NHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKL 543

Query: 545 IQGGDWLGASGVTF 549
           IQGG WL  +GVTF
Sbjct: 544 IQGGLWLKPTGVTF 553

[Close]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot
Match: P14280 (Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5)

HSP 1 Score: 553.9 bits (1426), Expect = 2.0e-156
Identity = 293/547 (53.56%), Postives = 373/547 (68.19%), Query Frame = 0


Query: 11  QPPKTTRSKTFSTILSFSAI-FCSAIFITNKLIESNPSQTVNDQPFLHTICHKAYNPSSC 70
           Q P   ++   + I+SF  + F   +F+   L+     +  +       +C  A +   C
Sbjct: 5   QQPLLIKTHKQNPIISFKILSFVITLFVALFLVAPYQVEIKHS-----NLCKTAQDSQLC 64

Query: 71  IEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRGEAALADC 130
           +   +        T   +G   L  FL   + ++ N I   R +  +IN  R   AL DC
Sbjct: 65  LSYVSDLISNEIVTTESDGHSILMKFLVNYVHQMNNAIPVVRKMKNQINDIRQHGALTDC 124

Query: 131 VELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIPRAAGMDLG 190
           +EL++ S+D  +DSI+A+  R+     NA +WLS VLTNH+TC DE++ S  +A      
Sbjct: 125 LELLDQSVDFASDSIAAIDKRSRSEHANAQSWLSGVLTNHVTCLDELD-SFTKAMINGTN 184

Query: 191 LEELILRGRNSLGILVSIWGSE----MEENGNGYPNWLKSGDRRLLGLMGREMEANIVVA 250
           LEELI R + +L +L S+   +    M   G   P+W+ S DR+L+   G+++ AN VVA
Sbjct: 185 LEELISRAKVALAMLASLTTQDEDVFMTVLGK-MPSWVSSMDRKLMESSGKDIIANAVVA 244

Query: 251 KDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDSTIIT 310
           +DG+G+++T+ EAV A PDKSK+R VIYVK+G Y+ENVE+   K N+MI+GDGM +T IT
Sbjct: 245 QDGTGDYQTLAEAVAAAPDKSKTRYVIYVKRGTYKENVEVASNKMNLMIVGDGMYATTIT 304

Query: 311 ASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINRCRID 370
            SLNVVDGSTTF+SAT+AAVG GFI QDI  +NTAGP K QAVALRVGAD +VINRCRID
Sbjct: 305 GSLNVVDGSTTFRSATLAAVGQGFILQDICIQNTAGPAKDQAVALRVGADMSVINRCRID 364

Query: 371 AYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQSRID 430
           AYQDTLY HS RQFYRD  +TGTVDFIFGNAAVV Q C++  R+P   Q NMVTAQ R D
Sbjct: 365 AYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKCQLVARKPGKYQQNMVTAQGRTD 424

Query: 431 PNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAGWAEW 490
           PNQ TGTSIQ C I+ASSDLEPV +   TYLGRPWKEYSRTVVM+S +GGLI+PAGWAEW
Sbjct: 425 PNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRPWKEYSRTVVMESYLGGLINPAGWAEW 484

Query: 491 NGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWLGASG 550
           +GDFALKTLYYGE+ NNGPG+  SKRVKWPGYHVIT P++A  FTV  LIQGG WL ++G
Sbjct: 485 DGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDPAKAMPFTVAKLIQGGSWLRSTG 544

Query: 551 VTFIPGL 553
           V ++ GL
Sbjct: 545 VAYVDGL 544

[Close]

BLAST of Lag0013722 vs. ExPASy TrEMBL
Match: A0A0A0KHV5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.3e-264
Identity = 462/558 (82.80%), Postives = 501/558 (89.78%), Query Frame = 0


Query: 3   MTTHQP--LLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTIC 62
           M THQP  LLQPPKT+RSKT  T+L F+A+ CSAIFITNK I+ NPS+  ND PFL  IC
Sbjct: 1   MATHQPLLLLQPPKTSRSKTIPTLLCFAAVLCSAIFITNKFIKPNPSE--NDLPFLQNIC 60

Query: 63  HKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIP 122
           HKAY+PSSCIEMAASEFPL    KT N   FLQ FL KS+ KVI+TIE A+D+ +RIN P
Sbjct: 61  HKAYDPSSCIEMAASEFPL-SIIKTTNEVDFLQSFLRKSMPKVISTIERAKDIRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGEAALADC+ELMEIS  RI DS+ ALKNRTSGSIEN+HTWLSSVLTNH+TC DEVE+S+
Sbjct: 121 RGEAALADCIELMEISNGRIMDSVLALKNRTSGSIENSHTWLSSVLTNHVTCWDEVESSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRRLLGLM 242
            RAA MDLGLEELI+RGRNSLG+LVSIWG       E+E+ GNGYP+WLK GDRRLLG++
Sbjct: 181 SRAAPMDLGLEELIMRGRNSLGMLVSIWGLDIKNLGELEKKGNGYPSWLKKGDRRLLGVL 240

Query: 243 GREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 302
           GREME NIVVAKDGSGNFKTVKEAVE++PDKSK+RIVIYVK+G YEENVE+GKKKKNVMI
Sbjct: 241 GREMEPNIVVAKDGSGNFKTVKEAVESVPDKSKNRIVIYVKRGTYEENVEVGKKKKNVMI 300

Query: 303 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 362
           +GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGPEKHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPEKHQAVALRVGA 360

Query: 363 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPM+NQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420

Query: 423 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 482
           ANMVTAQ RIDPNQNTGTSIQQC IVASSDLEPVK+SIKTYLGRPWKEYSRTV+MQS+IG
Sbjct: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVIMQSRIG 480

Query: 483 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 542
            LI PAGWAEWNGDFALKTLYYGEYSN GPGSDVSKRVKW GYH+ITSPSEAQKFTVDSL
Sbjct: 481 DLIQPAGWAEWNGDFALKTLYYGEYSNTGPGSDVSKRVKWDGYHIITSPSEAQKFTVDSL 540

Query: 543 IQGGDWLGASGVTFIPGL 553
           IQGG+WLG SG TFIPGL
Sbjct: 541 IQGGEWLGPSGATFIPGL 555

[Close]

BLAST of Lag0013722 vs. ExPASy TrEMBL
Match: A0A5D3CNY5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006640 PE=3 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.4e-261
Identity = 458/558 (82.08%), Postives = 495/558 (88.71%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CSAIF+TNK I+ NP +  ND PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYE--NDLPFLHNIC 60

Query: 63  HKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIP 122
           HKAYNPSSCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RIN P
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSGSIE++HTWLSSVLTNH+TC DEVE S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGSIESSHTWLSSVLTNHVTCWDEVETSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRRLLGLM 242
            RAA MDLGLEELI+RGRNSLG+LVSIWG       E+ + GNGYP+WLK GDRRLLG++
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVL 240

Query: 243 GREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 302
           GREME NIVVAKDGSGNFKTV+EAVE++PDKSK RIVIYVKKG Y+ENVE+GKKKKNVMI
Sbjct: 241 GREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKDRIVIYVKKGTYKENVEVGKKKKNVMI 300

Query: 303 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 362
           +GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGP KHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGA 360

Query: 363 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPM+NQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420

Query: 423 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 482
           ANMVTAQ RIDPNQNTGTSIQQC IVASSDLEPVK+SIKTYLGRPWKEYSRTVVMQS+IG
Sbjct: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASSDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIG 480

Query: 483 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 542
            LI PAGWAEW+GDFALKTLYYGEYSN GPGSDVSKRVKW GYHVITSPSEAQKFTV+SL
Sbjct: 481 DLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSL 540

Query: 543 IQGGDWLGASGVTFIPGL 553
           IQGG+WLG SG +FIPGL
Sbjct: 541 IQGGEWLGPSGASFIPGL 555

[Close]

BLAST of Lag0013722 vs. ExPASy TrEMBL
Match: A0A6J1KRW2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.6e-260
Identity = 457/551 (82.94%), Postives = 499/551 (90.56%), Query Frame = 0


Query: 3   MTTHQPLL-QPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICH 62
           M TH+PLL QPP+TTR KTF TIL FS +  SAIFITNKLI SNPS+  +  PFLH IC+
Sbjct: 1   MATHEPLLPQPPETTRPKTFFTILFFSLLLGSAIFITNKLINSNPSEN-DHHPFLHNICY 60

Query: 63  KAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPR 122
           KAYN SSCI+M ASEFPL QETKT N A+FLQ FL KS +K++NTIESA+DL RRIN PR
Sbjct: 61  KAYNQSSCIQMLASEFPLLQETKTTNAANFLQSFLRKSTSKIVNTIESAKDLRRRINNPR 120

Query: 123 GEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSIP 182
           GEAALADCVELMEISMDRI DSI AL+ R  G+IENAHTWLSSVLTNH+ CSDE+ENSI 
Sbjct: 121 GEAALADCVELMEISMDRITDSILALERRRCGTIENAHTWLSSVLTNHVACSDEIENSIS 180

Query: 183 RAAGMDLGLEELILRGRNSLGILVSIWGSEMEENGNGYPNWLKSGDRRLLGLMGREMEAN 242
           +AA MDLG+EELI+ GRNSLG+LVSIWGS  +E G GYP+WLK GDRRLL ++GR M+A+
Sbjct: 181 KAAAMDLGVEELIVIGRNSLGMLVSIWGSGKKE-GMGYPSWLKKGDRRLLEVVGRGMKAD 240

Query: 243 IVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDS 302
           IVVAKDGSGNFKTVKEAVEA+PDKSKSRIVIYVKKGIY+ENVE+GK KKNVM++GDGMDS
Sbjct: 241 IVVAKDGSGNFKTVKEAVEAVPDKSKSRIVIYVKKGIYKENVEVGKNKKNVMLVGDGMDS 300

Query: 303 TIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINR 362
           TIITASLNVVDGSTTFKSATVAAVGDGFIAQDI FRNTA PEKHQAVALRVGADQ+VINR
Sbjct: 301 TIITASLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAEPEKHQAVALRVGADQSVINR 360

Query: 363 CRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 422
           CRIDAYQDTLY H+NRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ
Sbjct: 361 CRIDAYQDTLYAHTNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQ 420

Query: 423 SRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAG 482
            R DPNQNTGTSIQ+C+IVAS DLEPVK+SIKTYLGRPWKEYSRTVVMQS +G LIDPAG
Sbjct: 421 GRTDPNQNTGTSIQRCEIVASPDLEPVKKSIKTYLGRPWKEYSRTVVMQSTLGDLIDPAG 480

Query: 483 WAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWL 542
           WAEWNG+FALKTLYYGEYSNNGPGS+VSKRVKWPGYHVITSP+ AQKFTVDSLIQG +WL
Sbjct: 481 WAEWNGEFALKTLYYGEYSNNGPGSNVSKRVKWPGYHVITSPAVAQKFTVDSLIQGAEWL 540

Query: 543 GASGVTFIPGL 553
           G+SGVTFIPGL
Sbjct: 541 GSSGVTFIPGL 549

[Close]

BLAST of Lag0013722 vs. ExPASy TrEMBL
Match: A0A1S3B018 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 7.8e-260
Identity = 456/558 (81.72%), Postives = 495/558 (88.71%), Query Frame = 0


Query: 3   MTTHQPL--LQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTIC 62
           M THQPL  LQPPKT+ SKT STIL F+ + CSAIF+TNK I+ NP +  ND PFLH IC
Sbjct: 1   MATHQPLLHLQPPKTSCSKTISTILCFAVVLCSAIFVTNKFIKLNPYE--NDLPFLHNIC 60

Query: 63  HKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIP 122
           HKAYNPSSCIEMAASEFPL    KT N  HFLQ FL KS+ KVINTIE A+DL +RIN P
Sbjct: 61  HKAYNPSSCIEMAASEFPL-SIIKTTNEEHFLQSFLKKSMPKVINTIERAKDLRQRINSP 120

Query: 123 RGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVENSI 182
           RGE AL DC+ELMEIS  RI DS+ ALKNRTSG IE++HTWLSSVLTNH+TC DEVE+S+
Sbjct: 121 RGEGALDDCIELMEISNGRIMDSVLALKNRTSGYIESSHTWLSSVLTNHVTCWDEVESSL 180

Query: 183 PRAAGMDLGLEELILRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRRLLGLM 242
            RAA MDLGLEELI+RGRNSLG+LVSIWG       E+ + GNGYP+WLK GDRRLLG++
Sbjct: 181 SRAAAMDLGLEELIMRGRNSLGMLVSIWGLEIKNLGELGKRGNGYPSWLKKGDRRLLGVL 240

Query: 243 GREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 302
           GREME NIVVAKDGSGNFKTV+EAVE++PDKSK+RIVIYVKKG Y+ENVE+GKKKKNVMI
Sbjct: 241 GREMEPNIVVAKDGSGNFKTVQEAVESVPDKSKNRIVIYVKKGTYKENVEVGKKKKNVMI 300

Query: 303 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 362
           +GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI F+NTAGP KHQAVALRVGA
Sbjct: 301 VGDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFQNTAGPAKHQAVALRVGA 360

Query: 363 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 422
           DQ+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIEPRRPM+NQ
Sbjct: 361 DQSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEPRRPMNNQ 420

Query: 423 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 482
           ANMVTAQ RIDPNQNTGTSIQQC IVAS DLEPVK+SIKTYLGRPWKEYSRTVVMQS+IG
Sbjct: 421 ANMVTAQGRIDPNQNTGTSIQQCDIVASFDLEPVKKSIKTYLGRPWKEYSRTVVMQSRIG 480

Query: 483 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 542
            LI PAGWAEW+GDFALKTLYYGEYSN GPGSDVSKRVKW GYHVITSPSEAQKFTV+SL
Sbjct: 481 DLIQPAGWAEWSGDFALKTLYYGEYSNTGPGSDVSKRVKWAGYHVITSPSEAQKFTVNSL 540

Query: 543 IQGGDWLGASGVTFIPGL 553
           IQGG+WLG SG +FIPGL
Sbjct: 541 IQGGEWLGPSGASFIPGL 555

[Close]

BLAST of Lag0013722 vs. ExPASy TrEMBL
Match: A0A6J1CN37 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 9.9e-247
Identity = 442/557 (79.35%), Postives = 476/557 (85.46%), Query Frame = 0


Query: 3   MTTHQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHTICHK 62
           M T+QPLLQPPKT RSK FS IL F+ I CSA FI N LI  NPS+   DQ FLH IC K
Sbjct: 1   MATNQPLLQPPKTHRSKAFSIILCFATILCSAFFIANHLIGPNPSEI--DQLFLHNICRK 60

Query: 63  AYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARD--LSRRINIP 122
           AYNP+SC+ MAASE P H+E K  NG HFLQ FL KSL KV +TIE A D  LSRRIN  
Sbjct: 61  AYNPASCVAMAASEVPFHREAKMTNGVHFLQVFLRKSLTKVTDTIERANDLILSRRINDA 120

Query: 123 RGEAALADCVELMEISMDRIADSI--SALKNRTSGSIENAHTWLSSVLTNHMTCSDEVEN 182
           RGEAALADC+ELMEISMDRI DSI   AL N+TS  IE+AH WLSSVLTNH+TCSDE+E 
Sbjct: 121 RGEAALADCLELMEISMDRITDSILALALNNKTSVPIEDAHAWLSSVLTNHVTCSDELEK 180

Query: 183 SIPRAAGMDLGLEELILRGRNSLGILVSIWGSEME---ENGNGYPNWLKSGDRRLLGLMG 242
           S+      +LGLEELI+  RNSL +LVSI   E E   E   G+P WLKSGDR LLG+ G
Sbjct: 181 SLLGTTAAELGLEELIMTARNSLAMLVSISQPEPEPEIEGQIGFPGWLKSGDRMLLGVTG 240

Query: 243 REMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMII 302
           ++++ANIVVAKDGSGNFKTVKEAV ++PDKSK+R VIYVKKG YEENVE+ KKKKNVMI+
Sbjct: 241 QQIKANIVVAKDGSGNFKTVKEAVASVPDKSKNRFVIYVKKGTYEENVEVEKKKKNVMIV 300

Query: 303 GDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGAD 362
           GDGMDSTIIT SLNVVDGSTTFKSATVAAVGDGFIAQDI FRNTAGPEKHQAVALRVGAD
Sbjct: 301 GDGMDSTIITGSLNVVDGSTTFKSATVAAVGDGFIAQDIWFRNTAGPEKHQAVALRVGAD 360

Query: 363 QAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQA 422
           Q+VINRCRIDAYQDTLYTHSNRQFYRD TITGTVDFIFGNAAVVLQNCKIE RRPMSNQA
Sbjct: 361 QSVINRCRIDAYQDTLYTHSNRQFYRDSTITGTVDFIFGNAAVVLQNCKIEARRPMSNQA 420

Query: 423 NMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGG 482
           NMVTAQ RIDPNQNTGTSI +CQIVASSDLEPVKRSIKTYLGRPWKEYSRTV+MQS+IG 
Sbjct: 421 NMVTAQGRIDPNQNTGTSIHRCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVIMQSRIGD 480

Query: 483 LIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLI 542
           LIDP GWAEWNGDFALKTLYYGEY NNGPGSDVSKRVKWPGY VIT+P+EAQKFTVDSLI
Sbjct: 481 LIDPTGWAEWNGDFALKTLYYGEYLNNGPGSDVSKRVKWPGYRVITNPAEAQKFTVDSLI 540

Query: 543 QGGDWLGASGVTFIPGL 553
           QGGDWLG +GVTFIPGL
Sbjct: 541 QGGDWLGPTGVTFIPGL 555

[Close]

BLAST of Lag0013722 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 562.8 bits (1449), Expect = 3.1e-160
Identity = 306/554 (55.23%), Postives = 382/554 (68.95%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIESNPSQTVNDQPFLHT--ICHK 64
           ++QPLL  PK+ + K    +LSF AI  S  F T +LI  N +   ND   L T  ICH 
Sbjct: 4   SNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNN--NDDSLLTTSQICHG 63

Query: 65  AYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPRG 124
           A++  SC +   SEF     +K  N    L  FL  S+ ++ +T+    +   R N  R 
Sbjct: 64  AHDQDSC-QALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGVRD 123

Query: 125 EAALADCVELMEISMDRIADSISALK--NRTSGSIENAHTWLSSVLTNHMTCSDEVEN-S 184
           +A  ADC E+M++S DR+  S+  L+  N    S  N HTWLSSVLTN+MTC + + + S
Sbjct: 124 KAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCLESISDVS 183

Query: 185 IPRAAGMDLGLEELILRGRNSLGILVSIWGSEMEEN---GNGYPNWLKSGDRRLLGLMGR 244
           +     +   LE+L+ R R +L I VS+  +  +      N +P+WL + DR+LL    +
Sbjct: 184 VNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDRKLLESSPK 243

Query: 245 EME--ANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMI 304
            ++  AN+VVAKDG+G FKTV EAV A P+ S +R VIYVKKG+Y+E ++IGKKKKN+M+
Sbjct: 244 TLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLML 303

Query: 305 IGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGA 364
           +GDG D+TIIT SLNV+DGSTTF+SATVAA GDGF+AQDI F+NTAGP KHQAVALRV A
Sbjct: 304 VGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSA 363

Query: 365 DQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQ 424
           DQ VINRCRIDAYQDTLYTH+ RQFYRD  ITGTVDFIFGN+AVV QNC I  R P + Q
Sbjct: 364 DQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQ 423

Query: 425 ANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIG 484
            NM+TAQ R D NQNT  SIQ+C+I ASSDL PVK S+KT+LGRPWK YSRTV+MQS I 
Sbjct: 424 KNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFID 483

Query: 485 GLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSL 544
             IDPAGW  W+G+FAL TLYYGEY+N GPG+D SKRV W G+ VI    EA++FTV  L
Sbjct: 484 NHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKL 543

Query: 545 IQGGDWLGASGVTF 549
           IQGG WL  +GVTF
Sbjct: 544 IQGGLWLKPTGVTF 553

[Close]

BLAST of Lag0013722 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 446.4 bits (1147), Expect = 3.3e-125
Identity = 253/572 (44.23%), Postives = 335/572 (58.57%), Query Frame = 0


Query: 5   THQPLLQPPKTTRSKTFSTILSFSAIFCSAIFITNKLIES---NPSQTVNDQPFLHTICH 64
           T  P LQ    T+   F+  +    + C  IF   + ++S    P  T      +   C 
Sbjct: 21  TSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAISRTCS 80

Query: 65  KAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTIESARDLSRRINIPR 124
           K+  P+ CI+    +FP    + T +    +    + +L K    + ++  ++     PR
Sbjct: 81  KSLYPNLCID-TLLDFP---GSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPPR 140

Query: 125 GEAALADCVELMEISMDRIADSISALKNRTSG-SIENAHTWLSSVLTNHMTCS---DEVE 184
             +A   C+EL++ S+D +  ++S++   +   S  +  TWLSS +TNH TC+   DE+E
Sbjct: 141 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 200

Query: 185 NSIPRAAGMDLG-LEELILRGRNSLGILV---------------SIWGSEMEENGNGYPN 244
                     +G +++L     N L I                  + G+E  E     PN
Sbjct: 201 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEE---LPN 260

Query: 245 WLKSGDRRLLGLMGREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIY-E 304
           WLK  DR LLG     ++A+I V+KDGSG FKT+ EA++  P+ S  R VIYVK G Y E
Sbjct: 261 WLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEE 320

Query: 305 ENVEIGKKKKNVMIIGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTA 364
           EN+++G+KK N+M IGDG   T+IT   ++ D  TTF +AT AA G GFI +D+ F N A
Sbjct: 321 ENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYA 380

Query: 365 GPEKHQAVALRVGADQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVL 424
           GP KHQAVALRVG D AV+ RC I  YQD LY HSNRQF+R+C I GTVDFIFGNAAV+L
Sbjct: 381 GPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVIL 440

Query: 425 QNCKIEPRRPMSNQANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPW 484
           Q+C I  R+PM+ Q   +TAQ+R DPNQNTG SI  C+++A+ DLE  K S  TYLGRPW
Sbjct: 441 QSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPW 500

Query: 485 KEYSRTVVMQSKIGGLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVI 544
           K YSR V M S +G  IDP GW EWNG FAL +LYYGEY N G GS + +RVKWPGYHVI
Sbjct: 501 KLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVI 560

Query: 545 TSPSEAQKFTVDSLIQGGDWLGASGVTFIPGL 553
           TS  EA KFTV   I G  WL ++GV+F  GL
Sbjct: 561 TSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of Lag0013722 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 431.8 bits (1109), Expect = 8.3e-121
Identity = 247/582 (42.44%), Postives = 341/582 (58.59%), Query Frame = 0


Query: 4   TTHQPLLQPPKTT--------RSK----TFSTILSFSAIFCSAIFI----TNKLIESNPS 63
           TT  P+L    T+        RSK      S +L+ S I  +AIF       KL +S P 
Sbjct: 19  TTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLNQSVPG 78

Query: 64  QTVNDQPFLHTICHKAYNPSSCIEMAASEFPLHQETKTKNGAHFLQFFLSKSLAKVINTI 123
                   +   C     P  C++ +  +FP        +    +   ++ +L    + +
Sbjct: 79  LARKPSQAISKACELTRFPELCVD-SLMDFP--GSLAASSSKDLIHVTVNMTLHHFSHAL 138

Query: 124 ESARDLSRRINIPRGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLT 183
            S+  LS     PR  +A   CVEL++ S+D ++ ++S++ + +S   ++  TWLS+ LT
Sbjct: 139 YSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVS-SSAKPQDVTTWLSAALT 198

Query: 184 NHMTCSDEVENSIPRAA--GMDLGLEELILRGRNSLGILVSIWGSE-------------- 243
           NH TC++  +          M   L+ L     N L I  +    +              
Sbjct: 199 NHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLG 258

Query: 244 MEENGNGYPNWLKSGDRRLLGLMGREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIV 303
           +EE    +P W++  +R +L +   +++A+I+V+KDG+G  KT+ EA++  P  S  RI+
Sbjct: 259 VEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRII 318

Query: 304 IYVKKGIYEE-NVEIGKKKKNVMIIGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFI 363
           IYVK G YEE N+++G+KK N+M +GDG   T+I+   ++ D  TTF +A+ AA G GFI
Sbjct: 319 IYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFI 378

Query: 364 AQDIGFRNTAGPEKHQAVALRVGADQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVD 423
           A+DI F N AGP KHQAVALR+GAD AVI RC I  YQDTLY HSNRQF+R+C I GTVD
Sbjct: 379 ARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVD 438

Query: 424 FIFGNAAVVLQNCKIEPRRPMSNQANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKR 483
           FIFGNAAVVLQNC I  R+PM  Q N +TAQ+R DPNQNTG SI   +++A+SDL+    
Sbjct: 439 FIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNG 498

Query: 484 SIKTYLGRPWKEYSRTVVMQSKIGGLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSK 543
           S +TYLGRPWK +SRTV M S IGG +   GW EWN  FAL TLYYGEY N+GPGS + +
Sbjct: 499 STQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYLNSGPGSGLGQ 558

Query: 544 RVKWPGYHVITSPSEAQKFTVDSLIQGGDWLGASGVTFIPGL 553
           RV WPGY VI S +EA +FTV   I G  WL ++GV+F+ GL
Sbjct: 559 RVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596

[Close]

BLAST of Lag0013722 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.4e-120
Identity = 236/479 (49.27%), Postives = 300/479 (62.63%), Query Frame = 0


Query: 120 PRGEAALADCVELMEISMDRIADSISAL-----KNRTSGSIENAHTWLSSVLTNHMTCSD 179
           PR + AL DC+E ++ ++D + +++  L     K        +  T +SS +TN  TC D
Sbjct: 121 PREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLD 180

Query: 180 EVENSIPRAAGMDLGLEELILRGR--------NSLGILVSIWGSEM-------------- 239
              +        D  + + +L+G+        N+L ++ ++  +++              
Sbjct: 181 GFSHD-----DADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNR 240

Query: 240 ---EEN----------------GNGYPNWLKSGDRRLLGLMGREMEANIVVAKDGSGNFK 299
              EEN                  G+P WL +GDRRL  L G  ++A+  VA DGSG FK
Sbjct: 241 KLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRL--LQGSGVKADATVAADGSGTFK 300

Query: 300 TVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKKKNVMIIGDGMDSTIITASLNVVDG 359
           TV  AV A P+ S  R VI++K G+Y ENVE+ KKKKN+M +GDG   TIIT S NVVDG
Sbjct: 301 TVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDG 360

Query: 360 STTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVALRVGADQAVINRCRIDAYQDTLYT 419
           STTF SATVAAVG+ F+A+DI F+NTAGP KHQAVALRVG+D +    C + AYQDTLY 
Sbjct: 361 STTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYV 420

Query: 420 HSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRRPMSNQANMVTAQSRIDPNQNTGTS 479
           HSNRQF+  C I GTVDFIFGNAAVVLQ+C I  RRP S Q NMVTAQ R DPNQNTG  
Sbjct: 421 HSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIV 480

Query: 480 IQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVMQSKIGGLIDPAGWAEWNGDFALKT 539
           IQ+C+I A+SDL+ VK S  TYLGRPWKEYS+TV+MQS I  +I P GW+EW G FAL T
Sbjct: 481 IQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNT 540

Query: 540 LYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKFTVDSLIQGGDWLGASGVTFIPGL 553
           L Y EYSN G G+  + RVKW G+ VIT+ +EAQK+T    I GG WL ++G  F  GL
Sbjct: 541 LTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Lag0013722 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 426.4 bits (1095), Expect = 3.5e-119
Identity = 240/503 (47.71%), Postives = 312/503 (62.03%), Query Frame = 0


Query: 60  CHKAYNPSSCIEMAASEFPLHQETK--TKNGAHFLQFFLSKSLAKVINTIESARDLSRRI 119
           C +  NP  C       F  H       K+ + FL+  +   L + I     A  L  + 
Sbjct: 34  CSQTPNPKPC-----EYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKC 93

Query: 120 NIPRGEAALADCVELMEISMDRIADSISALKNRTSGSIENAHTWLSSVLTNHMTCSDEVE 179
              R +AA  DC++L ++++ +I +++         S  +A TWLS+ LTN  TC     
Sbjct: 94  RDTREKAAWEDCIKLYDLTVSKINETMDP---NVKCSKLDAQTWLSTALTNLDTC----- 153

Query: 180 NSIPRAAGMDLGLEELI--LRGRNSLGILVSIWG------SEMEENGNGYPNWLKSGDRR 239
               RA  ++LG+ +++  L   N   +L +         +      +G+P+W+K GDR+
Sbjct: 154 ----RAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRK 213

Query: 240 LLGLMGREMEANIVVAKDGSGNFKTVKEAVEAIPDKSKSRIVIYVKKGIYEENVEIGKKK 299
           L  L     + N VVAKDGSGNFKT+KEA++A       R VIYVK+G+Y EN+EI  +K
Sbjct: 214 L--LQSSTPKDNAVVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RK 273

Query: 300 KNVMIIGDGMDSTIITASLNVVDGSTTFKSATVAAVGDGFIAQDIGFRNTAGPEKHQAVA 359
           KNVM+ GDG+  TIIT S +V  G+TTF SATVAAVGDGFIA+ I FRNTAG    QAVA
Sbjct: 274 KNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 333

Query: 360 LRVGADQAVINRCRIDAYQDTLYTHSNRQFYRDCTITGTVDFIFGNAAVVLQNCKIEPRR 419
           LR G+D +V  +C  +AYQDTLY HSNRQFYRDC + GTVDFIFGNAA VLQNC I  RR
Sbjct: 334 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARR 393

Query: 420 PMSNQANMVTAQSRIDPNQNTGTSIQQCQIVASSDLEPVKRSIKTYLGRPWKEYSRTVVM 479
           P S + N +TAQ R DPNQNTG  I   ++ A+SDL PV  S KTYLGRPW++YSRTV M
Sbjct: 394 PRS-KTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFM 453

Query: 480 QSKIGGLIDPAGWAEWNGDFALKTLYYGEYSNNGPGSDVSKRVKWPGYHVITSPSEAQKF 539
           ++ +  LIDP GW EW+G+FALKTL+Y E+ N GPG+  S RV WPG+ V+ S SEA KF
Sbjct: 454 KTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKF 511

Query: 540 TVDSLIQGGDWLGASGVTFIPGL 553
           TV + + GG W+  S V F  GL
Sbjct: 514 TVGTFLAGGSWI-PSSVPFTSGL 511

The following BLAST results are available for this feature:

BLAST of Lag0013722 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881670.1	4.2e-268	84.17	pectinesterase [Benincasa hispida]	[more]
XP_004134752.1	4.8e-264	82.80	pectinesterase [Cucumis sativus] >KGN49128.1 hypothetical protein Csa_004290 [Cu...	[more]
XP_023518219.1	1.1e-260	82.94	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
TYK13062.1	1.1e-260	82.08	pectinesterase [Cucumis melo var. makuwa]	[more]
XP_023002934.1	3.3e-260	82.94	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Lag0013722 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83947	7.2e-170	56.25	Pectinesterase/pectinesterase inhibitor OS=Ficus pumila var. awkeotsang OX=20423...	[more]
P09607	1.0e-160	53.99	Pectinesterase 2.1 OS=Solanum lycopersicum OX=4081 GN=PME2.1 PE=2 SV=2	[more]
Q96575	2.3e-160	53.99	Pectinesterase 2.2 OS=Solanum lycopersicum OX=4081 GN=PME2.2 PE=3 SV=1	[more]
Q1JPL7	4.4e-159	55.23	Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM...	[more]
P14280	2.0e-156	53.56	Pectinesterase 1 OS=Solanum lycopersicum OX=4081 GN=PME1.9 PE=1 SV=5	[more]

BLAST of Lag0013722 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHV5	2.3e-264	82.80	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G514890 PE=3 SV=1	[more]
A0A5D3CNY5	5.4e-261	82.08	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00664...	[more]
A0A6J1KRW2	1.6e-260	82.94	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496691 PE=3 SV=1	[more]
A0A1S3B018	7.8e-260	81.72	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103484595 PE=3 SV=1	[more]
A0A6J1CN37	9.9e-247	79.35	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111012508 PE=3 SV=1	[more]

BLAST of Lag0013722 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11580.1	3.1e-160	55.23	methylesterase PCR A	[more]
AT5G53370.1	3.3e-125	44.23	pectin methylesterase PCR fragment F	[more]
AT3G49220.1	8.3e-121	42.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14310.1	1.4e-120	49.27	pectin methylesterase 3	[more]
AT2G45220.1	3.5e-119	47.71	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 51..203 e-value: 3.7E-16 score: 69.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 55..179 e-value: 6.2E-19 score: 68.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 25..176 e-value: 1.5E-18 score: 64.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 52..204 e-value: 6.5E-25 score: 89.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 54..179
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 241..537 e-value: 1.4E-153 score: 509.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 235..552 e-value: 5.9E-127 score: 425.4
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 17..551
None	No IPR available	PANTHER	PTHR31707:SF333	PECTINESTERASE	coord: 17..551
None	No IPR available	CDD	cd15799	PMEI-like_4	coord: 59..207 e-value: 4.84633E-28 score: 107.496
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 385..394
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 239..551

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013722.1	Lag0013722.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity