Lag0013624 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013624
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA oxidative demethylase ALKBH2
Location	chr1: 51653475 .. 51654674 (-)
RNA-Seq Expression	Lag0013624
Synteny	Lag0013624

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTTGAGGTTTAAAGAAGTGGCCAATTCAGAATCGGAGACGACGATCCCTCCTCCTATCGATTCAGGAAGGCGACAGACTTTGAATCTCGGAAATGGAAGCGAAATCGTCTACATTAGCAAGTTCGTGCCCTCCGATCAGGCTTGGACGTGGTTCGATTTCCTCAATCAACGCATTCCTTGGACCAGGCCGACCATTCGCGTCTTCGGTCGCTCCGTCGTCCAGGTCTTTCCCTTTCTCCATTCTCTTCTCTCTTTTCGGTTTGTTGTCGTGCTAATTAGAAATTGACGGTTCAACGATTGACCGTTGGTTGTCTTAGATGATTTTTTCTTCTTTCCCGCCATTCTTAGAATTGGGGTTTTGTGTTCTTTCTGAAGAACCGTAGAGCATTTTTCACGCGATTGAGGGTAACATTTGAGTTTTAATTTGCTAATGCAGCCCAGAGATACTTGCTACGTTGCGAGTCCTGGATTGACGACATTAACTTATAGTGGGTATAAACCCCATGCCTATACTTGGGATGATTTTCCTCCTCTTAAGGACATTTTGAATGCTGTAAGACTTTTTGATTCGATCTTTCACTATCAGGATATTTTGGCCTTTTAAACTACAAGTTCTCTTTACCAGTGGCTCCTAATGTGTTTTCTTCAATTTTCTCTGTGAAGGTCCACGAAGCTCTTCCAGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGCAACGATTATGTGGGCTGGCATTCTGATGATGAGAAACTATATGCATTGAACCAGGAGATTGCTTCTGTATCACTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCACCAAAACTTCTCAACGTATTCCGTCTATTTCATATGTTTTTGTTGTTTCTGTTCTGATTTTGAAAAGCCCTTTTCTCAAATCTTCACTATGTTGCTCAATTGCTTTCGTCACTTCAAAAAGTTCCGCCATTTTATGGTTTATTTGATGTGGCCCCAGGACGAAATGAAGAGGAACCTCCAAGCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCATTTGTACTAAAGCATGGTTCTCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAGGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTGTTGCTGATTGA

mRNA sequence

ATGACTTTGAGGTTTAAAGAAGTGGCCAATTCAGAATCGGAGACGACGATCCCTCCTCCTATCGATTCAGGAAGGCGACAGACTTTGAATCTCGGAAATGGAAGCGAAATCGTCTACATTAGCAAGTTCGTGCCCTCCGATCAGGCTTGGACGTGGTTCGATTTCCTCAATCAACGCATTCCTTGGACCAGGCCGACCATTCGCGTCTTCGGTCGCTCCGTCGTCCAGCCCAGAGATACTTGCTACGTTGCGAGTCCTGGATTGACGACATTAACTTATAGTGGGTATAAACCCCATGCCTATACTTGGGATGATTTTCCTCCTCTTAAGGACATTTTGAATGCTGTCCACGAAGCTCTTCCAGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGCAACGATTATGTGGGCTGGCATTCTGATGATGAGAAACTATATGCATTGAACCAGGAGATTGCTTCTGTATCACTTGGATCCCTTTTCTCAAATCTTCACTATGTTGCTCAATTGCTTTCGTCACTTCAAAAAGTTCCGCCATTTTATGGTTTATTTGATGTGGCCCCAGGACGAAATGAAGAGGAACCTCCAAGCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCATTTGTACTAAAGCATGGTTCTCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAGGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTGTTGCTGATTGA

Coding sequence (CDS)

ATGACTTTGAGGTTTAAAGAAGTGGCCAATTCAGAATCGGAGACGACGATCCCTCCTCCTATCGATTCAGGAAGGCGACAGACTTTGAATCTCGGAAATGGAAGCGAAATCGTCTACATTAGCAAGTTCGTGCCCTCCGATCAGGCTTGGACGTGGTTCGATTTCCTCAATCAACGCATTCCTTGGACCAGGCCGACCATTCGCGTCTTCGGTCGCTCCGTCGTCCAGCCCAGAGATACTTGCTACGTTGCGAGTCCTGGATTGACGACATTAACTTATAGTGGGTATAAACCCCATGCCTATACTTGGGATGATTTTCCTCCTCTTAAGGACATTTTGAATGCTGTCCACGAAGCTCTTCCAGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGCAACGATTATGTGGGCTGGCATTCTGATGATGAGAAACTATATGCATTGAACCAGGAGATTGCTTCTGTATCACTTGGATCCCTTTTCTCAAATCTTCACTATGTTGCTCAATTGCTTTCGTCACTTCAAAAAGTTCCGCCATTTTATGGTTTATTTGATGTGGCCCCAGGACGAAATGAAGAGGAACCTCCAAGCAAGAAATCAAAGAAAAGCAGTGTGGCTGATACACACTCATTTGTACTAAAGCATGGTTCTCTTTTGGTTATGAAAGGCTACACTCAACGAGATTGGATGCATTCAGTGCCTAAGCGCCTCAGGGCAGAGGCAACCCGCATCAACCTTACTTTCAGGCACGTTGTTGCTGATTGA

Protein sequence

MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVVAD

Homology

[Close]

BLAST of Lag0013624 vs. NCBI nr
Match: XP_038882915.1 (DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882917.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882918.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882919.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882920.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882921.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida])

HSP 1 Score: 427.9 bits (1099), Expect = 6.0e-116
Identity = 208/258 (80.62%), Postives = 225/258 (87.21%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LR KEV +SESET IP P +SGRRQTL+LGNGSEIVY+ KFVPSDQAWTWFDFLNQRI
Sbjct: 1   MSLRLKEVTDSESETKIPRPFNSGRRQTLDLGNGSEIVYVPKFVPSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSV+QPRDTCYVA+PGLTTLTYSGYKPHAYTWDDFPPLKDIL+AVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFPPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVS G     L         L+K
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFL---------LKK 180

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
            P        +  RN+EEPP+KKSKKSSVAD+HSFVLKHGSLLVM+GYTQRDWMHSVPKR
Sbjct: 181 KPN-----KTSQRRNDEEPPAKKSKKSSVADSHSFVLKHGSLLVMRGYTQRDWMHSVPKR 240

Query: 241 LRAEATRINLTFRHVVAD 259
           L+AEATRINLTFRHV+ D
Sbjct: 241 LKAEATRINLTFRHVIVD 244

[Close]

BLAST of Lag0013624 vs. NCBI nr
Match: XP_022133026.1 (DNA oxidative demethylase ALKBH2 [Momordica charantia])

HSP 1 Score: 424.1 bits (1089), Expect = 8.7e-115
Identity = 208/258 (80.62%), Postives = 222/258 (86.05%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           MTLR KEV +SESET IP P+D GRR TL+LGNGSEIVY++KFVPSD+AWTWFDFLNQRI
Sbjct: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYVASPGLTTLTYSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G     L         L+K
Sbjct: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFL---------LKK 188

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
            P        +  RN+EEPPSKKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 189 KPN-----KTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKR 248

Query: 241 LRAEATRINLTFRHVVAD 259
           L+AEATRINLTFRHVV D
Sbjct: 249 LKAEATRINLTFRHVVVD 252

[Close]

BLAST of Lag0013624 vs. NCBI nr
Match: KAG6603836.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-114
Identity = 207/255 (81.18%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P+D GRRQTL+LGNGSEIVYISKFV SDQAWTWFDFLNQ I
Sbjct: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYV +PGLTT +YSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G       ++ +  S+   
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFG---CERDFLLKKKSN--- 180

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
                     +  RN+EEPP+KKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 181 --------KTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240

Query: 241 LRAEATRINLTFRHV 256
           L+AEATRINLTFRHV
Sbjct: 241 LKAEATRINLTFRHV 241

[Close]

BLAST of Lag0013624 vs. NCBI nr
Match: XP_022950477.1 (DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 DNA oxidative demethylase ALKBH2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-114
Identity = 207/255 (81.18%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P+D GRRQTL+LGNGSEIVYISKFV SDQAWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYV +PGLTT +YSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G       ++ +  S+   
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFG---CERDFLLKKKSN--- 194

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
                     +  RN+EEPP+KKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 195 --------KTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 254

Query: 241 LRAEATRINLTFRHV 256
           L+AEATRINLTFRHV
Sbjct: 255 LKAEATRINLTFRHV 255

[Close]

BLAST of Lag0013624 vs. NCBI nr
Match: XP_004134811.1 (DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742940.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >KGN49032.1 hypothetical protein Csa_003044 [Cucumis sativus])

HSP 1 Score: 419.1 bits (1076), Expect = 2.8e-113
Identity = 206/258 (79.84%), Postives = 221/258 (85.66%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P D G+RQTL+LGNGSEI+YISKFV SDQAWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTI VFGRSV+QPRDTCYVA+PGLTTLTYSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVS G     L         L+K
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFL---------LKK 180

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
            P        +  RN+EEPP+KKSKKSSVAD HSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 181 KPN-----KTSQRRNDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240

Query: 241 LRAEATRINLTFRHVVAD 259
           L+AEATRINLTFRHV+ D
Sbjct: 241 LKAEATRINLTFRHVIVD 244

[Close]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 1.7e-76
Identity = 137/240 (57.08%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 18  PPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQP 77
           P   ++  R+T++LG+GS+++YI +F+P  Q+WT+FD+L++ IPWTRPTIRVFGRS +QP
Sbjct: 88  PSSSNAPSRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQP 147

Query: 78  RDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGN 137
           RDTCYVAS GLT L YSGY+P +Y+WDDFPPLK+IL+A+++ LPGS FNSLLLNRYKG +
Sbjct: 148 RDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGAS 207

Query: 138 DYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEE 197
           DYV WH+DDEK+Y    EIASVS G       +V +     +           + G+  +
Sbjct: 208 DYVAWHADDEKIYGPTPEIASVSFG---CERDFVLKKKKDEES----------SQGKTGD 267

Query: 198 EPPSKKS-KKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVV 257
             P+KK  K+SS  D  S  LKHGSLLVM+GYTQRDW+HSVPKR +AE TRINLTFR V+
Sbjct: 268 SGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

[Close]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot
Match: Q32L00 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.0e-17
Identity = 73/228 (32.02%), Postives = 100/228 (43.86%), Query Frame = 0


Query: 35  SEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYS 94
           S +     FV   +A + F+ L + +PW + T      +  QPR T +    G    TYS
Sbjct: 88  SRVCLCPGFVDLKEADSVFEQLCRDVPWKQRTGIRDDVTYQQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLY 154
                  PH   W   P L  + N + E   G SFNSLL N Y+   D V WHSDDE   
Sbjct: 148 RITMEPNPH---W--HPVLLMLKNQIEEN-TGHSFNSLLCNLYRNEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEEEPPSKKSKKSSVA 214
                IAS+S G+                       +F++      ++PP + +   +  
Sbjct: 208 GRCPIIASLSFGAT---------------------RMFEM-----RKKPPPEDNGDYTYV 267

Query: 215 DTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVVAD 259
           +     L HG+LL+M+G TQ DW H VPK   +   RINLTFR V  D
Sbjct: 268 ERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRINLTFRTVYPD 280

[Close]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot
Match: Q96Q83 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606 GN=ALKBH3 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.0e-17
Identity = 71/228 (31.14%), Postives = 100/228 (43.86%), Query Frame = 0


Query: 35  SEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYS 94
           S +     FV   +A    + L Q +PW + T      +  QPR T +    G    TYS
Sbjct: 88  SRVCLYPGFVDVKEADWILEQLCQDVPWKQRTGIREDITYQQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLY 154
                  PH   W   P L+ + N + E   G +FNSLL N Y+   D V WHSDDE   
Sbjct: 148 RITMEPNPH---W--HPVLRTLKNRIEEN-TGHTFNSLLCNLYRNEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEEEPPSKKSKKSSVA 214
                IAS+S G+  +                     F++      ++PP +++   +  
Sbjct: 208 GRCPIIASLSFGATRT---------------------FEM-----RKKPPPEENGDYTYV 267

Query: 215 DTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVVAD 259
           +     L HG+LL+M+G TQ DW H VPK   +   R+NLTFR V  D
Sbjct: 268 ERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPD 280

[Close]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot
Match: Q58DM4 (DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 61/190 (32.11%), Postives = 88/190 (46.32%), Query Frame = 0


Query: 67  IRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFN 126
           ++VFG+    PR        GL T T+SG       W   P L+ + + V   + G +FN
Sbjct: 98  VQVFGKWHSVPRKQATYGDTGL-TYTFSGLTLSPKPW--IPVLERVRDRV-SLVTGQTFN 157

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYG 186
            +L+NRYK G D++G H DDE+  AL   IASVS G+    +                  
Sbjct: 158 FVLINRYKDGQDHIGEHRDDERELALGSPIASVSFGACRDFVF----------------- 217

Query: 187 LFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEAT 246
                  R+++      S++  V       L HGSLL+M   T   W HS+P R +  A 
Sbjct: 218 -------RHKDSRGKHPSRRLEVV---RLQLAHGSLLMMNHPTNTHWYHSLPVRKKVLAP 256

Query: 247 RINLTFRHVV 257
           R+NLTFR ++
Sbjct: 278 RVNLTFRKIL 256

[Close]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot
Match: Q5XIC8 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Rattus norvegicus OX=10116 GN=Alkbh3 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.1e-15
Identity = 69/228 (30.26%), Postives = 98/228 (42.98%), Query Frame = 0


Query: 35  SEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYS 94
           S +     FV   +A    + L Q +PW +        +  QPR T +    G    TYS
Sbjct: 88  SRVCLYPGFVDLKEADWILERLCQDVPWKQRMGIREDITYPQPRLTAWY---GELPYTYS 147

Query: 95  GY----KPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLY 154
                  PH   W   P L  + + + E   G +FNSLL N Y+   D V WHSDDE   
Sbjct: 148 RVTMEPNPH---W--LPVLWTLKSRIEEN-TGHTFNSLLCNFYRDEKDSVDWHSDDEPSL 207

Query: 155 ALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEEEPPSKKSKKSSVA 214
                IAS+S G+  +                     F++      ++PP +++   +  
Sbjct: 208 GSCPVIASLSFGATRT---------------------FEM-----RKKPPPEENGDYTYV 267

Query: 215 DTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVVAD 259
           +     L HG+LL+M+G TQ DW H VPK   +   R+NLTFR V  D
Sbjct: 268 ERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSRERRVNLTFRTVYPD 280

[Close]

BLAST of Lag0013624 vs. ExPASy TrEMBL
Match: A0A6J1BTV9 (DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 4.2e-115
Identity = 208/258 (80.62%), Postives = 222/258 (86.05%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           MTLR KEV +SESET IP P+D GRR TL+LGNGSEIVY++KFVPSD+AWTWFDFLNQRI
Sbjct: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYVASPGLTTLTYSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G     L         L+K
Sbjct: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFL---------LKK 188

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
            P        +  RN+EEPPSKKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 189 KPN-----KTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKR 248

Query: 241 LRAEATRINLTFRHVVAD 259
           L+AEATRINLTFRHVV D
Sbjct: 249 LKAEATRINLTFRHVVVD 252

[Close]

BLAST of Lag0013624 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.5e-115
Identity = 207/255 (81.18%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P+D GRRQTL+LGNGSEIVYISKFV SDQAWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYV +PGLTT +YSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G       ++ +  S+   
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFG---CERDFLLKKKSN--- 194

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
                     +  RN+EEPP+KKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 195 --------KTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 254

Query: 241 LRAEATRINLTFRHV 256
           L+AEATRINLTFRHV
Sbjct: 255 LKAEATRINLTFRHV 255

[Close]

BLAST of Lag0013624 vs. ExPASy TrEMBL
Match: A0A0A0KL20 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510990 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.4e-113
Identity = 206/258 (79.84%), Postives = 221/258 (85.66%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P D G+RQTL+LGNGSEI+YISKFV SDQAWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTI VFGRSV+QPRDTCYVA+PGLTTLTYSGYKPHAYTWDDF PLKDIL+AVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVS G     L         L+K
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFL---------LKK 180

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
            P        +  RN+EEPP+KKSKKSSVAD HSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 181 KPN-----KTSQRRNDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240

Query: 241 LRAEATRINLTFRHVVAD 259
           L+AEATRINLTFRHV+ D
Sbjct: 241 LKAEATRINLTFRHVIVD 244

[Close]

BLAST of Lag0013624 vs. ExPASy TrEMBL
Match: A0A6J1GEX8 (DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.4e-113
Identity = 205/253 (81.03%), Postives = 219/253 (86.56%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P+D GRRQTL+LGNGSEIVYISKFV SDQAWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYV +PGLTT +YSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G       ++ +  S+   
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFG---CERDFLLKKKSN--- 194

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
                     +  RN+EEPP+KKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 195 --------KTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 253

Query: 241 LRAEATRINLTFR 254
           L+AEATRINLTFR
Sbjct: 255 LKAEATRINLTFR 253

[Close]

BLAST of Lag0013624 vs. ExPASy TrEMBL
Match: A0A6J1IQU8 (DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.7e-113
Identity = 204/253 (80.63%), Postives = 218/253 (86.17%), Query Frame = 0


Query: 1   MTLRFKEVANSESETTIPPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRI 60
           M+LRFKEV +SESET IP P+D GRRQTL+LGNGSEIVYISKFV SDQAWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSVVQPRDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEAL 120
           PWTRPTIRVFGRSVVQPRDTCYV +PGLT  +YSGYKPHAYTWDDFPPLKDILNAVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTIYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQK 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVS G       ++ +  S+   
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFG---CERDFLLKKKSN--- 194

Query: 181 VPPFYGLFDVAPGRNEEEPPSKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 240
                     +  RN+EEPP+KKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR
Sbjct: 195 --------KTSQRRNDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKR 253

Query: 241 LRAEATRINLTFR 254
           L+AEATRINLTFR
Sbjct: 255 LKAEATRINLTFR 253

[Close]

BLAST of Lag0013624 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.2e-77
Identity = 137/240 (57.08%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 18  PPPIDSGRRQTLNLGNGSEIVYISKFVPSDQAWTWFDFLNQRIPWTRPTIRVFGRSVVQP 77
           P   ++  R+T++LG+GS+++YI +F+P  Q+WT+FD+L++ IPWTRPTIRVFGRS +QP
Sbjct: 88  PSSSNAPSRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQP 147

Query: 78  RDTCYVASPGLTTLTYSGYKPHAYTWDDFPPLKDILNAVHEALPGSSFNSLLLNRYKGGN 137
           RDTCYVAS GLT L YSGY+P +Y+WDDFPPLK+IL+A+++ LPGS FNSLLLNRYKG +
Sbjct: 148 RDTCYVASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGAS 207

Query: 138 DYVGWHSDDEKLYALNQEIASVSLGSLFSNLHYVAQLLSSLQKVPPFYGLFDVAPGRNEE 197
           DYV WH+DDEK+Y    EIASVS G       +V +     +           + G+  +
Sbjct: 208 DYVAWHADDEKIYGPTPEIASVSFG---CERDFVLKKKKDEES----------SQGKTGD 267

Query: 198 EPPSKKS-KKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLRAEATRINLTFRHVV 257
             P+KK  K+SS  D  S  LKHGSLLVM+GYTQRDW+HSVPKR +AE TRINLTFR V+
Sbjct: 268 SGPAKKRLKRSSREDQQSLTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

The following BLAST results are available for this feature:

BLAST of Lag0013624 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882915.1	6.0e-116	80.62	DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidati...	[more]
XP_022133026.1	8.7e-115	80.62	DNA oxidative demethylase ALKBH2 [Momordica charantia]	[more]
KAG6603836.1	1.1e-114	81.18	DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022950477.1	1.1e-114	81.18	DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 D...	[more]
XP_004134811.1	2.8e-113	79.84	DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative...	[more]

BLAST of Lag0013624 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	1.7e-76	57.08	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]
Q32L00	1.0e-17	32.02	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 G...	[more]
Q96Q83	3.0e-17	31.14	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Homo sapiens OX=9606...	[more]
Q58DM4	5.6e-16	32.11	DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1	[more]
Q5XIC8	2.1e-15	30.26	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Rattus norvegicus OX...	[more]

BLAST of Lag0013624 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTV9	4.2e-115	80.62	DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 ...	[more]
A0A6J1GEX9	5.5e-115	81.18	DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A0A0KL20	1.4e-113	79.84	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1GEX8	1.4e-113	81.03	DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IQU8	6.7e-113	80.63	DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=...	[more]

BLAST of Lag0013624 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22260.1	1.2e-77	57.08	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 25..258 e-value: 1.1E-53 score: 184.1
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 37..253 e-value: 4.0E-27 score: 95.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 34..255
IPR032852	DNA oxidative demethylase ALKBH2	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 22..256
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 124..256 score: 11.755658

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013624.1	Lag0013624.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0032259	methylation
biological_process	GO:0035511	oxidative DNA demethylation
biological_process	GO:0006281	DNA repair
molecular_function	GO:0051747	cytosine C-5 DNA demethylase activity
molecular_function	GO:0043734	DNA-N1-methyladenine dioxygenase activity
molecular_function	GO:0008198	ferrous iron binding
molecular_function	GO:0008168	methyltransferase activity