Lag0013493 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013493
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA-directed DNA polymerase (reverse transcriptase)-related family protein
Locationchr1: 50741511 .. 50742701 (+)
RNA-Seq ExpressionLag0013493
SyntenyLag0013493
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTGCTTTCGTCTTCCTTCTAACATTTGTTATGCCATTGACAGGTTATGCGCCAACTTTTGGTGGGGTTCGGTGGGAAACAAATCCAAATGTCACTGGATGAACCGGAAGAAAATGTGCTCAAACAAGGAAGAGGGAGGTTTGGGATTTAGACAACTCTCTTTGTTCAACCAAGCTATGTTGGCTAAGCTTAGCTGGAGAATTTTGGGCGACCAAAATAGCCTTATTTTCAAAGTGTTAAGGGGGAGATACTTCAAAGACTCGTGCTTCCTAAAGGCCAAGCTAAGGAATGACTCTTCCCTCACCTGGAGAAGTATCTGTTGGGGAAGAGATCTTTTTAAAAAAGGCTATCGTTGGAAAGTGGGCAACGAGAAGCAGATTGTGATCGACAAAGATCTGTGGATAGGAGGCAACAATCTACACCCAAGGATTATGAATGATTCCCTCAAAGATAATTATGTTAGTAGCCTTATTGAGGAGAATATGTCCGGGAAATAAGATCTTATCAAGGCTAGTTTCTCCCCTGAAGCTGCTGACGACATCTTGAAGATCCCTTTGGGCGATATCAATTCAAAGGACAAGATTATCTGGACTTATGACAAAAAAGGAACCTTCTCTGTGAAGAGTGCCTATAGGCTAGCCCAAGCCAATTGCCCTTTACAAGAGACTTCTCAATCTGACACTTCAAAGACGGTTGGTCTTTGGAAAAACCTCCGGAAAACAGACACTATCCCGAGAGCTAAGCTGTGCGCGTGGATGATTATCAACAATATCATTTCTACCAAAGCTAACATTGCTTGAAAAGAAATAGCCATCATCCCTATTTGTGACCTGTGTAGGAAGCATTATGAGACCTCTAGCCACACCATTTGGGAGTGTAAGCTTTCTAAAGGAATTTGGAAAATTTTCATTCCGTCCTCATTGGGGATTTTTTCTCTTTGCAGGGAAGATTGGTCTCCCATGGACTACTGGAATTGGTTGGTGGACAACGGTAGTAGAGAAAATCTTTCAAAAGAAGTTCTCATTATGTGGTCAATCTGGAATTTTAGAAACAAATCGAGGGCTCTCAAGCTAAAACCAGAATCAATCTACTCATCTATTGAAGCTATTTTAGAAGAAAATGAGTCTAAGAACCTGATTTCTCGGAAGCCCGTCAGGCTGAGGAGCCAAATGAGTCAAGTTCCCTAG

mRNA sequence

ATGAGTTGCTTTCGTCTTCCTTCTAACATTTGTTATGCCATTGACAGGTTATGCGCCAACTTTTGGTGGGGTTCGGTGGGAAACAAATCCAAATGTCACTGGATGAACCGGAAGAAAATGTGCTCAAACAAGGAAGAGGGAGGTTTGGGATTTAGACAACTCTCTTTGTTCAACCAAGCTATGTTGGCTAAGCTTAGCTGGAGAATTTTGGGCGACCAAAATAGCCTTATTTTCAAAGTGTTAAGGGGGAGATACTTCAAAGACTCGTGCTTCCTAAAGGCCAAGCTAAGGAATGACTCTTCCCTCACCTGGAGAAGTATCTGTTGGGGAAGAGATCTTTTTAAAAAAGGCTATCGTTGGAAAGTGGGCAACGAGAAGCAGATTGTGATCGACAAAGATCTGTGGATAGGAGGCAACAATCTACACCCAAGGATTATGAATGATTCCCTCAAAGATAATTATATCCCTTTGGGCGATATCAATTCAAAGGACAAGATTATCTGGACTTATGACAAAAAAGGAACCTTCTCTGTGAAGAGTGCCTATAGGCTAGCCCAAGCCAATTGCCCTTTACAAGAGACTTCTCAATCTGACACTTCAAAGACGGTTGGTCTTTGGAAAAACCTCCGGAAAACAGACACTATCCCGAGAGCTAAGCTGGAAGATTGGTCTCCCATGGACTACTGGAATTGGTTGGTGGACAACGGTAGTAGAGAAAATCTTTCAAAAGAAGTTCTCATTATGTGGTCAATCTGGAATTTTAGAAACAAATCGAGGGCTCTCAAGCTAAAACCAGAATCAATCTACTCATCTATTGAAGCTATTTTAGAAGAAAATGAGTCTAAGAACCTGATTTCTCGGAAGCCCGTCAGGCTGAGGAGCCAAATGAGTCAAGTTCCCTAG

Coding sequence (CDS)

ATGAGTTGCTTTCGTCTTCCTTCTAACATTTGTTATGCCATTGACAGGTTATGCGCCAACTTTTGGTGGGGTTCGGTGGGAAACAAATCCAAATGTCACTGGATGAACCGGAAGAAAATGTGCTCAAACAAGGAAGAGGGAGGTTTGGGATTTAGACAACTCTCTTTGTTCAACCAAGCTATGTTGGCTAAGCTTAGCTGGAGAATTTTGGGCGACCAAAATAGCCTTATTTTCAAAGTGTTAAGGGGGAGATACTTCAAAGACTCGTGCTTCCTAAAGGCCAAGCTAAGGAATGACTCTTCCCTCACCTGGAGAAGTATCTGTTGGGGAAGAGATCTTTTTAAAAAAGGCTATCGTTGGAAAGTGGGCAACGAGAAGCAGATTGTGATCGACAAAGATCTGTGGATAGGAGGCAACAATCTACACCCAAGGATTATGAATGATTCCCTCAAAGATAATTATATCCCTTTGGGCGATATCAATTCAAAGGACAAGATTATCTGGACTTATGACAAAAAAGGAACCTTCTCTGTGAAGAGTGCCTATAGGCTAGCCCAAGCCAATTGCCCTTTACAAGAGACTTCTCAATCTGACACTTCAAAGACGGTTGGTCTTTGGAAAAACCTCCGGAAAACAGACACTATCCCGAGAGCTAAGCTGGAAGATTGGTCTCCCATGGACTACTGGAATTGGTTGGTGGACAACGGTAGTAGAGAAAATCTTTCAAAAGAAGTTCTCATTATGTGGTCAATCTGGAATTTTAGAAACAAATCGAGGGCTCTCAAGCTAAAACCAGAATCAATCTACTCATCTATTGAAGCTATTTTAGAAGAAAATGAGTCTAAGAACCTGATTTCTCGGAAGCCCGTCAGGCTGAGGAGCCAAATGAGTCAAGTTCCCTAG

Protein sequence

MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQAMLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRWKVGNEKQIVIDKDLWIGGNNLHPRIMNDSLKDNYIPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKNLRKTDTIPRAKLEDWSPMDYWNWLVDNGSRENLSKEVLIMWSIWNFRNKSRALKLKPESIYSSIEAILEENESKNLISRKPVRLRSQMSQVP
Homology
BLAST of Lag0013493 vs. NCBI nr
Match: XP_030479238.1 (uncharacterized protein LOC115696478 [Cannabis sativa])

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-44
Identity = 105/254 (41.34%), Postives = 138/254 (54.33%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCF+LP++ C+ I+RL A FWWGS+G  SK HW N + +CS+K  GGLGFR L   NQA
Sbjct: 712 MSCFKLPASTCHKIERLMAQFWWGSMGKGSKIHWKNWRTLCSSKFFGGLGFRSLISHNQA 771

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           MLAK +WR+L + NSL+  +L+ +YFK + FL+AKL +  S TW S+ WGRDL  +G  W
Sbjct: 772 MLAKQAWRVLSNPNSLLTSILKAKYFKLNNFLEAKLGHTPSYTWSSLIWGRDLLIRGLLW 831

Query: 121 KVGNEKQIVIDKDLWIGG--------NNLHPRIMNDSLKDN------------------- 180
           KVGN   I   +D WI G        ++L P        DN                   
Sbjct: 832 KVGNGCSIRTFQDPWIPGIKFPCLRSSDLPPADKVSFFIDNSGFWDRKKLQQYFDEYSVL 891

Query: 181 ---YIPLGDINSKDKIIWTYDKKGTFSVKSAYRLA-QANCPLQETSQSDTSKTVGLWKNL 224
               IP+G +   D +IW  +  G FSVKSAY +A  A+ P    S SD+S     WK L
Sbjct: 892 TILKIPIGGLQKADSLIWNQENSGIFSVKSAYHIANNASLP---PSSSDSSLPKAWWKTL 951

BLAST of Lag0013493 vs. NCBI nr
Match: XP_030494695.1 (uncharacterized protein LOC115710475 [Cannabis sativa])

HSP 1 Score: 187.2 bits (474), Expect = 2.1e-43
Identity = 106/254 (41.73%), Postives = 141/254 (55.51%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCF+LP++IC+ I+RL A FWWGS+G  SK HW N + +CS+K  GGLGFR L   NQA
Sbjct: 343 MSCFKLPASICHKIERLMAQFWWGSMGKGSKIHWKNWRVLCSSKFFGGLGFRSLISHNQA 402

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           MLAK + R   + NSL+  +L+ +YFK + FL+AKL +  S TW S+ WGRDL  +G  W
Sbjct: 403 MLAKQALRGFSNPNSLLANILKAKYFKLNNFLEAKLGHSPSYTWSSLLWGRDLLIRGLLW 462

Query: 121 KVGNEKQIVIDKDLWIGG--------NNLHP------RIMNDSLKDNY------------ 180
           KVGN   I   +D WI G        N+L P       I N+   D Y            
Sbjct: 463 KVGNGCSIRTFQDPWILGIKFPCLRSNDLPPVDKVSFFIDNNGFWDRYKLHQYFDDYSVL 522

Query: 181 ----IPLGDINSKDKIIWTYDKKGTFSVKSAYRLA-QANCPLQETSQSDTSKTVGLWKNL 224
               +P+G ++  D IIW  +  G FSVKSAY +A  A+ P    S S++S +   WK L
Sbjct: 523 SILKVPIGGLHKADSIIWNQENSGIFSVKSAYHIANNASLP---PSSSNSSLSNAWWKTL 582

BLAST of Lag0013493 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 184.5 bits (467), Expect = 1.3e-42
Identity = 93/240 (38.75%), Postives = 128/240 (53.33%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCFRLP  +      + A FWWGS     K HW+    +   K EGG+GFR L LFN+A
Sbjct: 598 MSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMGFRDLELFNKA 657

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           +LAK  WRIL   NS++ +VL+GRYFKD  F++AK+  + S  WRSI WGRDL KKG RW
Sbjct: 658 LLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKGLRW 717

Query: 121 KVGNEKQIVIDKDLWIGGNNL-----HPRI-----------------MNDSLKDNY---- 180
           ++GN   + I  D W+           PR+                   D ++D +    
Sbjct: 718 RIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFTPDE 777

Query: 181 ------IPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKN 209
                 IP+G    +D++IW Y+K G +SV+S Y++A  N P  +   S +S+ V  W N
Sbjct: 778 AKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWN 837

BLAST of Lag0013493 vs. NCBI nr
Match: XP_022155286.1 (uncharacterized protein LOC111022423 [Momordica charantia])

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-42
Identity = 98/215 (45.58%), Postives = 126/215 (58.60%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           +SCF+LP +IC  + ++   FWWGS     K HW + K +C  K+ GG+GFR +S+FNQA
Sbjct: 470 LSCFKLPVSICNELHQIYNRFWWGSSSEDRKIHWQSWKLLCIRKDLGGMGFRDISIFNQA 529

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           MLAK SWRIL   +SL+ K LRG+YFK   FL+AKL    S  WRSI WGRDLFKKGYRW
Sbjct: 530 MLAKQSWRILRHPDSLLAKTLRGKYFKTGSFLQAKLGARPSYAWRSILWGRDLFKKGYRW 589

Query: 121 KVGNEKQIVIDKDLWI--GGN------NLHPRIMN-----------DSLK--DNYI---- 180
           KVGN   I +  D W+   GN      ++H R  +           D +K  +++I    
Sbjct: 590 KVGNGFSINLSSDPWLPRDGNFSPVFTHIHVRNFSVAWLMEGRGRWDEIKVRESFIVSEA 649

Query: 181 ------PLGDINSKDKIIWTYDKKGTFSVKSAYRL 185
                 PL   +  D+IIW  DK G FSV+SAY L
Sbjct: 650 DIILQTPLPSQDKDDEIIWGMDKTGIFSVRSAYHL 684

BLAST of Lag0013493 vs. NCBI nr
Match: XP_030479464.1 (uncharacterized protein LOC115696715 [Cannabis sativa])

HSP 1 Score: 183.3 bits (464), Expect = 3.0e-42
Identity = 103/253 (40.71%), Postives = 132/253 (52.17%), Query Frame = 0

Query: 1    MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
            MSCFRLP +IC+ I++L A FWWGS+G  SK HW +   +C +K  GGLGFR L   NQA
Sbjct: 1274 MSCFRLPVSICHKIEKLMAQFWWGSMGKGSKIHWKSWNHLCVSKFFGGLGFRSLVHHNQA 1333

Query: 61   MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
            MLAK +W I  + NSL+F VL+ +YF  S FL A L +  S TWRS+ WGRDL KKG  W
Sbjct: 1334 MLAKQAWHIYSNPNSLLFVVLKAKYFHHSTFLDADLGHTPSFTWRSVLWGRDLLKKGLLW 1393

Query: 121  KVGNEKQIVIDKDLWI---------GGNN---------LHPR-IMNDSLKDNY------- 180
            KVGN + I   +D W+          G +         LHP    N    + Y       
Sbjct: 1394 KVGNGETIRTVEDFWVPEVRKIQYKAGCSPPSDKVSFFLHPEGYWNREALNTYFDPPTVS 1453

Query: 181  ----IPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKNLR 224
                +P+G  + +D +IW     G  SVKSAY +A A      +S S  SK    W  L 
Sbjct: 1454 SILHVPIGGCDKEDFLIWNNHPSGILSVKSAYHIANAGSLPPSSSNSSLSKK--WWTTLW 1513

BLAST of Lag0013493 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.0e-24
Identity = 56/137 (40.88%), Postives = 79/137 (57.66%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKE-EGGLGFRQLSLFNQ 60
           MSCFRL   +C  +      FWW S  NK K  W+  +K+C +KE +GGLGFR L  FNQ
Sbjct: 8   MSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQ 67

Query: 61  AMLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYR 120
           A+LAK S+RI+   ++L+ ++LR RYF  S  ++  +    S  WRSI  GR+L  +G  
Sbjct: 68  ALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLL 127

Query: 121 WKVGNEKQIVIDKDLWI 137
             +G+     +  D WI
Sbjct: 128 RTIGDGIHTKVWLDRWI 144

BLAST of Lag0013493 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.2e-23
Identity = 73/223 (32.74%), Postives = 109/223 (48.88%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MS   LP +I   +D+L   F WGS   K K H +   K+CS K+EGGLG R     N+A
Sbjct: 53  MSTILLPQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRA 112

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRY----FKDSCFLKAKLRNDSSLTWRSICWG-RDLFK 120
           +++K+ WR+L ++NSL   VL+ +Y     +DS +L  K     S TWRSI  G RD+  
Sbjct: 113 LISKVGWRLLQEKNSLWTLVLQKKYHVGEIRDSRWLIPK--GSWSSTWRSIAIGLRDVVS 172

Query: 121 KGYRWKVGNEKQI---------------------------VIDKDLWIGGN-----NLHP 180
            G  W  G+ +QI                           V+ KDLWI G       + P
Sbjct: 173 HGVGWIPGDGQQIRFWTDRWVSGKPLLELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDP 232

Query: 181 RIMNDSLKDNYIPLGDI--NSKDKIIWTYDKKGTFSVKSAYRL 185
              N++  +    + D+   ++D++ W + + G FSV+SAY +
Sbjct: 233 YTTNNTRLELRAVVLDLVTGARDRLSWKFSQDGQFSVRSAYEM 273

BLAST of Lag0013493 vs. ExPASy TrEMBL
Match: A0A803QI38 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 3.5e-44
Identity = 102/253 (40.32%), Postives = 133/253 (52.57%), Query Frame = 0

Query: 1    MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
            MSCF+LP++ C+ I+RL A FWWGS+G  SK HW N + +CS+K  GGLGF+ L   NQA
Sbjct: 1221 MSCFKLPASTCHKIERLMAQFWWGSMGKGSKIHWKNWRSLCSSKFFGGLGFKSLISHNQA 1280

Query: 61   MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
            MLAK +WR+L +  SL+  +L+ +YFK + FL+AKL +  S TW S+ WGRDL  +G  W
Sbjct: 1281 MLAKQAWRVLSNPTSLLACILKAKYFKLNNFLEAKLGHTPSYTWSSLIWGRDLLVRGLLW 1340

Query: 121  KVGNEKQIVIDKDLWIGGNNLHPRIMNDSLKDN--------------------------- 180
            KVGN   I   +D WI G        +D   D+                           
Sbjct: 1341 KVGNGCSIRTFQDPWIPGMKYPSLRSSDQPPDDKVSFFIDNSGFWDREKLQQYFDEYSVS 1400

Query: 181  ---YIPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKNLR 224
                IP+G     D +IWT D  G FSVKSAY +A        +S S  SKT   WK L 
Sbjct: 1401 TILKIPIGGPQKTDSLIWTQDNSGIFSVKSAYHIANNTSMPPSSSDSSLSKT--WWKTLW 1460

BLAST of Lag0013493 vs. ExPASy TrEMBL
Match: A0A803PV88 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 7.7e-44
Identity = 101/253 (39.92%), Postives = 136/253 (53.75%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCFRLP++ C  I++L A FWWGS+G  S+ HW + + +CS+K  GGLGFR L   NQA
Sbjct: 612 MSCFRLPASTCQKIEKLMAQFWWGSMGKGSQIHWKSWRTLCSSKFFGGLGFRSLVAHNQA 671

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           MLAK +WR+L +  SL+  VL+ +YF  + FLKAKL +  S TW S+ WGRDL K G  W
Sbjct: 672 MLAKQAWRVLSNPTSLLATVLKAKYFHHNDFLKAKLGHSPSYTWSSLLWGRDLLKHGLVW 731

Query: 121 KVGNEKQIVIDKDLWI--------GGNNLHPRIMNDSLKD----------NY-------- 180
           K+GN   +   +D WI        G N+L P    D   D          NY        
Sbjct: 732 KIGNGCSVRTFEDPWIPDMKSPCLGSNDLPPVETVDFFIDHQGFWNRDKLNYYFDNLSVS 791

Query: 181 ----IPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKNLR 224
               +P+G ++  D ++W +D  G F+VKSAY L  AN      S S+ S  +  WK   
Sbjct: 792 SILRVPIGGLHRDDTLVWKHDPSGVFTVKSAYHL--ANSTSLPPSSSNPSFFMSWWKTFW 851

BLAST of Lag0013493 vs. ExPASy TrEMBL
Match: A0A803NMW0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.3e-43
Identity = 102/268 (38.06%), Postives = 145/268 (54.10%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCFR+P ++C  I+RL A FWWGS GN+ K HW +  K+C +K  GG+GFR     NQA
Sbjct: 623 MSCFRVPVSVCKRIERLMAQFWWGSSGNQGKVHWNSWDKLCQSKFLGGMGFRSFVCHNQA 682

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
            LAK +WR+    NSL+ K+L+ +YF  + FL+A   + +S TWRS+ WG DL K+G  W
Sbjct: 683 FLAKQAWRVFSMPNSLLAKLLKAKYFSHNSFLEASKGHCASFTWRSLLWGGDLLKRGLIW 742

Query: 121 KVGNEKQIVIDKDLWIGGNNLHPRIMNDSLKDNYI---PLGDINSKDKIIWTYDKKGTFS 180
           KVGN + I      WI G      +  D      I   PL DI+  D ++W +   G F+
Sbjct: 743 KVGNGRSISSIDSYWIPGWREKLAMFFDEEVIQVILTVPLADISLDDTLVWEHHSSGLFT 802

Query: 181 VKSAYRLAQANCPLQETSQS---------DTSKTVGLWKNLRKTDTIPRAKLEDWSPMDY 240
           V SAY LA++   L  +S S         + S+ +  WK+ R  D    A+  +    +Y
Sbjct: 803 VNSAYHLAKSVNSLPSSSSSPKFVTHALLECSRALREWKDSRFKDFYRVAR--NCDIREY 862

Query: 241 WNWLVDNGSRENLSKEVLIMWSIWNFRN 257
                 + S+ +L   + I+W IWN RN
Sbjct: 863 LLRGFQDFSKADLRVFIGIVWEIWNRRN 888

BLAST of Lag0013493 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 6.5e-43
Identity = 93/240 (38.75%), Postives = 128/240 (53.33%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           MSCFRLP  +      + A FWWGS     K HW+    +   K EGG+GFR L LFN+A
Sbjct: 598 MSCFRLPKRLIREFHHITARFWWGSSKEDKKIHWVAWNSLYLPKCEGGMGFRDLELFNKA 657

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           +LAK  WRIL   NS++ +VL+GRYFKD  F++AK+  + S  WRSI WGRDL KKG RW
Sbjct: 658 LLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKGLRW 717

Query: 121 KVGNEKQIVIDKDLWIGGNNL-----HPRI-----------------MNDSLKDNY---- 180
           ++GN   + I  D W+           PR+                   D ++D +    
Sbjct: 718 RIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFTPDE 777

Query: 181 ------IPLGDINSKDKIIWTYDKKGTFSVKSAYRLAQANCPLQETSQSDTSKTVGLWKN 209
                 IP+G    +D++IW Y+K G +SV+S Y++A  N P  +   S +S+ V  W N
Sbjct: 778 AKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWN 837

BLAST of Lag0013493 vs. ExPASy TrEMBL
Match: A0A6J1DRA0 (uncharacterized protein LOC111022423 OS=Momordica charantia OX=3673 GN=LOC111022423 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 8.5e-43
Identity = 98/215 (45.58%), Postives = 126/215 (58.60%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           +SCF+LP +IC  + ++   FWWGS     K HW + K +C  K+ GG+GFR +S+FNQA
Sbjct: 470 LSCFKLPVSICNELHQIYNRFWWGSSSEDRKIHWQSWKLLCIRKDLGGMGFRDISIFNQA 529

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           MLAK SWRIL   +SL+ K LRG+YFK   FL+AKL    S  WRSI WGRDLFKKGYRW
Sbjct: 530 MLAKQSWRILRHPDSLLAKTLRGKYFKTGSFLQAKLGARPSYAWRSILWGRDLFKKGYRW 589

Query: 121 KVGNEKQIVIDKDLWI--GGN------NLHPRIMN-----------DSLK--DNYI---- 180
           KVGN   I +  D W+   GN      ++H R  +           D +K  +++I    
Sbjct: 590 KVGNGFSINLSSDPWLPRDGNFSPVFTHIHVRNFSVAWLMEGRGRWDEIKVRESFIVSEA 649

Query: 181 ------PLGDINSKDKIIWTYDKKGTFSVKSAYRL 185
                 PL   +  D+IIW  DK G FSV+SAY L
Sbjct: 650 DIILQTPLPSQDKDDEIIWGMDKTGIFSVRSAYHL 684

BLAST of Lag0013493 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 115.5 bits (288), Expect = 7.2e-26
Identity = 56/137 (40.88%), Postives = 79/137 (57.66%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKE-EGGLGFRQLSLFNQ 60
           MSCFRL   +C  +      FWW S  NK K  W+  +K+C +KE +GGLGFR L  FNQ
Sbjct: 8   MSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQ 67

Query: 61  AMLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYR 120
           A+LAK S+RI+   ++L+ ++LR RYF  S  ++  +    S  WRSI  GR+L  +G  
Sbjct: 68  ALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLL 127

Query: 121 WKVGNEKQIVIDKDLWI 137
             +G+     +  D WI
Sbjct: 128 RTIGDGIHTKVWLDRWI 144

BLAST of Lag0013493 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 9.7e-23
Identity = 50/136 (36.76%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSVGNKSKCHWMNRKKMCSNKEEGGLGFRQLSLFNQA 60
           M+CF LP  +C  I  + A+FWW +       HW     +   K EGG+GF+ +  FN A
Sbjct: 8   MACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLA 67

Query: 61  MLAKLSWRILGDQNSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRW 120
           +L K  WR+L    SL+ KV + RYF  S  L A L +  S  W+SI   +++ ++G R 
Sbjct: 68  LLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARA 127

Query: 121 KVGNEKQIVIDKDLWI 137
            VGN + I+I +  W+
Sbjct: 128 VVGNGEDIIIWRHKWL 143

BLAST of Lag0013493 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 40/137 (29.20%), Postives = 55/137 (40.15%), Query Frame = 0

Query: 81  LRGRYFKDSCFLKAKLRNDSSLTWRSICWGRDLFKKGYRWKVGNEKQIVIDKDLWIGG-- 140
           ++ RYFKD   L AK+R   S  W S+  G  L KKG R  +G+ + I I  D  +    
Sbjct: 1   MKARYFKDVSILDAKVRKQQSYGWASLLDGIALLKKGTRHLIGDGQNIRIGLDNIVDSHP 60

Query: 141 ---------------NNLHPR-----IMNDSLKDNYIPLGD-----------INSKDKII 185
                          NNL  R       +DS    ++   D               DKII
Sbjct: 61  PRPLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKII 120

BLAST of Lag0013493 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.7e-06
Identity = 36/116 (31.03%), Postives = 55/116 (47.41%), Query Frame = 0

Query: 1   MSCFRLPSNICYAIDRLCANFWWGSV---GNKSKCHWMNRKKMCSNKEEGGLGFRQLSLF 60
           MS FRLPS     ID +C++F W        K+K  W +   +C+ K+EGGLG R L   
Sbjct: 82  MSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSD---VCTPKDEGGLGIRSLKEA 141

Query: 61  NQAMLAKLSWRILGDQ--NSLIFKVLRGRYFKDSCFLKAKLRNDSSLTWRSICWGR 112
           N+       W I G+    S ++K +       S F+K  + N S+ ++    W +
Sbjct: 142 NKGSF----WSISGNTTLGSWMWKKILKHRALASGFVKHDIHNGSNTSFWFDNWSK 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_030479238.11.1e-4441.34uncharacterized protein LOC115696478 [Cannabis sativa][more]
XP_030494695.12.1e-4341.73uncharacterized protein LOC115710475 [Cannabis sativa][more]
XP_022150918.11.3e-4238.75uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_022155286.11.8e-4245.58uncharacterized protein LOC111022423 [Momordica charantia][more]
XP_030479464.13.0e-4240.71uncharacterized protein LOC115696715 [Cannabis sativa][more]
Match NameE-valueIdentityDescription
P932951.0e-2440.88Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
P0C2F63.2e-2332.74Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A803QI383.5e-4440.32Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PV887.7e-4439.92Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803NMW01.3e-4338.06Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DAR46.5e-4338.75uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1DRA08.5e-4345.58uncharacterized protein LOC111022423 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
Match NameE-valueIdentityDescription
ATMG00310.17.2e-2640.88RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT4G29090.19.7e-2336.76Ribonuclease H-like superfamily protein [more]
AT3G09510.12.6e-0729.20Ribonuclease H-like superfamily protein [more]
AT3G24255.11.7e-0631.03RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33116:SF41SUBFAMILY NOT NAMEDcoord: 1..188
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013493.1Lag0013493.1mRNA