Lag0013415 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013415
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr1: 50044517 .. 50046994 (-)
RNA-Seq ExpressionLag0013415
SyntenyLag0013415
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATCACTCTAACAGCACAGTTTCCATTAGAATCAACATGGGCTCAGACCATATCACTGCTCGAAAACTGCTCAAACATGAAGCAATTGAAACAAATTCACGCTCAAATGATCAAAACAGAGACCATTGCAGACCCCCAGTTAGCCACCAAGCTTCTAACTCTCTGCACTTCACCCCATTTCGGCGATTTGCATTACGCTCAAAAGATCTTCAATGGAATCACCAGCCCCACCACTTTCATGTGGAACGCCATTATAAGAGCGTATTCGAACAGTAACGAACCAGAATTGGCATTTCTCTTGTATCGTCAGATGCTTTCTTCTTCTGTGCCGCACAATTCTTACACCTTCCCTTTCTTGCTCAAAGCTTGTCGCAATTTGTCGGCCATGGGAGAGGCCCTTCAAGTCCATGGACTGATGATGAAATTGGGATTTGGGTCGGATGTTTTTGCTTTGAATTCTCTGCTTTATGTCTATGCTTTGTGTGGTGACATTCAGTATGCACGCCAACTGTTTGATAATATTCCTGAAAGAGATGTCGTTTCTTGGAACACTATGATCGATGGGTATATCAAATTTGGGGACGTAGAAACGGCTTATGGGGTTTTCTTGGACATGCCAATGAAGAATTTGGTCTCGTGGACGTCGATGATTTCGGGGATTGTTGAGGCAGGATTGAACGTGGAAGCTTTGAATCTTTGTTATGAAATGCAGAGGGCGGGATTTGAACTTGATGGGGTTGTTATTGCGAGTTTGCTCACTGCTTGTGCAAATCTTGGAGCGTTGGATCAAGGAAGATGGCTACATTTCTATGTGCTCAACAATGGTGTCCATATAGATAAAGTTATTGGCTGCGCTCTGGTGAATATGTACTTGAAATGTGGGGATATGGAAGAAGCCTTGCAGGTGTTTGGGAAACTGAAGGATGATCAGAAAGATGTGTATGTTTGGACGGCCATGATTGATGGTTTTGCCATCCATGGGCGTGGAGTGGAAGCTCTGGAATGGTTTGACAGAATGGTGAAAGAAGGAATAAGACCAAATTCCATCACTTTCACTGCAATTTTGACGGCCTGCAGCTATGCAGGACTGGTTGAAGAAGGAAAAGTTTTATTCAGGAGCATGAAAAGTCTCTATAACTTGAGCCCATCTATTGAGCATTATGGGTGTATGGTTGATCTTTTGGGTCGAGCTGGGCTGCTGGAGGAGGCTAAGGAGTTGATCAAGAAGATGCCCATGAAACCTAATGCTGTAATATGGGGAGCTTTGCTAAAGGTAAGGAAAGGGTACAGAGGCAGTCTCTAGTGATTAAAAAACTTTAATTTAGTTCACTCGCATTTTAACATTTTTCAATTACTTTTACTTTGGGTTAGTGGATCAGATTTAAGTATGGGTGATGTTACATTTTATCTGTCATAATACTCAATAAAATGATAGAAACTTAGAGGAAGAATCAAGCTATCTAGGGGAAAGAGTCGAATATTCACCCAATTTAAAATTTTGTTAGAAGAATTACAAATTTTCCTCATCTTTTAAGTAACTTCATTTCTCCTCTAAATTTGTAATTGTCTTTCGGCAGCCACGTTTGATATTTATCCTAGAATTGGTAGGATATTAGAACTTCTTTAGACTACATTAGGGACTATAGTCTCATGTTTGAGATAACAAATTAAGAACTTAAATTTCAAAACCTTAACTCTTTTATATAATTTTCTTAATCTAAGATAGTTGGGGGCATTTGTCACATTCGTTCCTCCTTGTTTAGGCCTGTCGGATTCATGGAAATTTTCTGCTCGGCAGCCAAATCGGAGCCCACCTGGTGGAAGTTGATTCAGATCACAGCGGCCGGTACATTCACTTGGCTAGCATTCTTGCTGCAGAAGGTAAATGGAAAGAAGCAGCTGAAGTGAGGTTGAAGATGAAGAATCTCAGAGTCCCAATTCCTCCAGGAAAGAGTTTGATAACTTTGAATGGCGTTGTTCATGAATTTCTTGCTGGGCATCAAGATCATCCACAGATGGAACAGATTCATCAGAAGTTGAACCAGGTTGCAGAGAGGCTCCGACAAGAAGAAGGGTAATCTTAAATCACCGTTTAATCTAAAAACTTAAGTTAATAGGTTACAGTAAATATAATCTAACACTTTCATTAATATTCTTTTTTTGAACAACCACTTTCATTAATATTCTTTTTAACAGATATGTGCCTAAGACTAAAGATTTATTGCTTGATCTTGAGAGTGAGGAGAAAGAAACTGCAATGGCACAACATAGTGAGAAGCTGGCGATTGCGTTTGGATTGATCAATACAAAACCAGGAACGACAATTCGAGTTGTTAAGAATCTTAGGGTATGTGAAGATTGTCACACTGTTGCGAAACTCGTATCTCGGATCTATGGTAGAGAGATTATAATGCGAGATAGAGTTCGATTCCACCATTTTAGAGATGGGAGTTGCTCTTGCAGAGATTACTGGTAG

mRNA sequence

ATGATCACTCTAACAGCACAGTTTCCATTAGAATCAACATGGGCTCAGACCATATCACTGCTCGAAAACTGCTCAAACATGAAGCAATTGAAACAAATTCACGCTCAAATGATCAAAACAGAGACCATTGCAGACCCCCAGTTAGCCACCAAGCTTCTAACTCTCTGCACTTCACCCCATTTCGGCGATTTGCATTACGCTCAAAAGATCTTCAATGGAATCACCAGCCCCACCACTTTCATGTGGAACGCCATTATAAGAGCGTATTCGAACAGTAACGAACCAGAATTGGCATTTCTCTTGTATCGTCAGATGCTTTCTTCTTCTGTGCCGCACAATTCTTACACCTTCCCTTTCTTGCTCAAAGCTTGTCGCAATTTGTCGGCCATGGGAGAGGCCCTTCAAGTCCATGGACTGATGATGAAATTGGGATTTGGGTCGGATGTTTTTGCTTTGAATTCTCTGCTTTATGTCTATGCTTTGTGTGGTGACATTCAGTATGCACGCCAACTGTTTGATAATATTCCTGAAAGAGATGTCGTTTCTTGGAACACTATGATCGATGGGTATATCAAATTTGGGGACGTAGAAACGGCTTATGGGGTTTTCTTGGACATGCCAATGAAGAATTTGGTCTCGTGGACGTCGATGATTTCGGGGATTGTTGAGGCAGGATTGAACGTGGAAGCTTTGAATCTTTGTTATGAAATGCAGAGGGCGGGATTTGAACTTGATGGGGTTGTTATTGCGAGTTTGCTCACTGCTTGTGCAAATCTTGGAGCGTTGGATCAAGGAAGATGGCTACATTTCTATGTGCTCAACAATGGTGTCCATATAGATAAAGTTATTGGCTGCGCTCTGGTGAATATGTACTTGAAATGTGGGGATATGGAAGAAGCCTTGCAGGTGTTTGGGAAACTGAAGGATGATCAGAAAGATGTGTATGTTTGGACGGCCATGATTGATGGTTTTGCCATCCATGGGCGTGGAGTGGAAGCTCTGGAATGGTTTGACAGAATGGTGAAAGAAGGAATAAGACCAAATTCCATCACTTTCACTGCAATTTTGACGGCCTGCAGCTATGCAGGACTGGTTGAAGAAGGAAAAGTTTTATTCAGGAGCATGAAAAGTCTCTATAACTTGAGCCCATCTATTGAGCATTATGGGTGTATGGTTGATCTTTTGGGTCGAGCTGGGCTGCTGGAGGAGGCTAAGGAGTTGATCAAGAAGATGCCCATGAAACCTAATGCTGTAATATGGGGAGCTTTGCTAAAGGCCTGTCGGATTCATGGAAATTTTCTGCTCGGCAGCCAAATCGGAGCCCACCTGGTGGAAGTTGATTCAGATCACAGCGGCCGGTACATTCACTTGGCTAGCATTCTTGCTGCAGAAGGTAAATGGAAAGAAGCAGCTGAAGTGAGGTTGAAGATGAAGAATCTCAGAGTCCCAATTCCTCCAGGAAAGAGTTTGATAACTTTGAATGGCGTTGTTCATGAATTTCTTGCTGGGCATCAAGATCATCCACAGATGGAACAGATTCATCAGAAGTTGAACCAGGTTGCAGAGAGGCTCCGACAAGAAGAAGGATATGTGCCTAAGACTAAAGATTTATTGCTTGATCTTGAGAGTGAGGAGAAAGAAACTGCAATGGCACAACATAGTGAGAAGCTGGCGATTGCGTTTGGATTGATCAATACAAAACCAGGAACGACAATTCGAGTTGTTAAGAATCTTAGGGTATGTGAAGATTGTCACACTGTTGCGAAACTCGTATCTCGGATCTATGGTAGAGAGATTATAATGCGAGATAGAGTTCGATTCCACCATTTTAGAGATGGGAGTTGCTCTTGCAGAGATTACTGGTAG

Coding sequence (CDS)

ATGATCACTCTAACAGCACAGTTTCCATTAGAATCAACATGGGCTCAGACCATATCACTGCTCGAAAACTGCTCAAACATGAAGCAATTGAAACAAATTCACGCTCAAATGATCAAAACAGAGACCATTGCAGACCCCCAGTTAGCCACCAAGCTTCTAACTCTCTGCACTTCACCCCATTTCGGCGATTTGCATTACGCTCAAAAGATCTTCAATGGAATCACCAGCCCCACCACTTTCATGTGGAACGCCATTATAAGAGCGTATTCGAACAGTAACGAACCAGAATTGGCATTTCTCTTGTATCGTCAGATGCTTTCTTCTTCTGTGCCGCACAATTCTTACACCTTCCCTTTCTTGCTCAAAGCTTGTCGCAATTTGTCGGCCATGGGAGAGGCCCTTCAAGTCCATGGACTGATGATGAAATTGGGATTTGGGTCGGATGTTTTTGCTTTGAATTCTCTGCTTTATGTCTATGCTTTGTGTGGTGACATTCAGTATGCACGCCAACTGTTTGATAATATTCCTGAAAGAGATGTCGTTTCTTGGAACACTATGATCGATGGGTATATCAAATTTGGGGACGTAGAAACGGCTTATGGGGTTTTCTTGGACATGCCAATGAAGAATTTGGTCTCGTGGACGTCGATGATTTCGGGGATTGTTGAGGCAGGATTGAACGTGGAAGCTTTGAATCTTTGTTATGAAATGCAGAGGGCGGGATTTGAACTTGATGGGGTTGTTATTGCGAGTTTGCTCACTGCTTGTGCAAATCTTGGAGCGTTGGATCAAGGAAGATGGCTACATTTCTATGTGCTCAACAATGGTGTCCATATAGATAAAGTTATTGGCTGCGCTCTGGTGAATATGTACTTGAAATGTGGGGATATGGAAGAAGCCTTGCAGGTGTTTGGGAAACTGAAGGATGATCAGAAAGATGTGTATGTTTGGACGGCCATGATTGATGGTTTTGCCATCCATGGGCGTGGAGTGGAAGCTCTGGAATGGTTTGACAGAATGGTGAAAGAAGGAATAAGACCAAATTCCATCACTTTCACTGCAATTTTGACGGCCTGCAGCTATGCAGGACTGGTTGAAGAAGGAAAAGTTTTATTCAGGAGCATGAAAAGTCTCTATAACTTGAGCCCATCTATTGAGCATTATGGGTGTATGGTTGATCTTTTGGGTCGAGCTGGGCTGCTGGAGGAGGCTAAGGAGTTGATCAAGAAGATGCCCATGAAACCTAATGCTGTAATATGGGGAGCTTTGCTAAAGGCCTGTCGGATTCATGGAAATTTTCTGCTCGGCAGCCAAATCGGAGCCCACCTGGTGGAAGTTGATTCAGATCACAGCGGCCGGTACATTCACTTGGCTAGCATTCTTGCTGCAGAAGGTAAATGGAAAGAAGCAGCTGAAGTGAGGTTGAAGATGAAGAATCTCAGAGTCCCAATTCCTCCAGGAAAGAGTTTGATAACTTTGAATGGCGTTGTTCATGAATTTCTTGCTGGGCATCAAGATCATCCACAGATGGAACAGATTCATCAGAAGTTGAACCAGGTTGCAGAGAGGCTCCGACAAGAAGAAGGATATGTGCCTAAGACTAAAGATTTATTGCTTGATCTTGAGAGTGAGGAGAAAGAAACTGCAATGGCACAACATAGTGAGAAGCTGGCGATTGCGTTTGGATTGATCAATACAAAACCAGGAACGACAATTCGAGTTGTTAAGAATCTTAGGGTATGTGAAGATTGTCACACTGTTGCGAAACTCGTATCTCGGATCTATGGTAGAGAGATTATAATGCGAGATAGAGTTCGATTCCACCATTTTAGAGATGGGAGTTGCTCTTGCAGAGATTACTGGTAG

Protein sequence

MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPHFGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDVVSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRAGFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLDLESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREIIMRDRVRFHHFRDGSCSCRDYW
Homology
BLAST of Lag0013415 vs. NCBI nr
Match: XP_022978438.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita maxima])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 547/621 (88.08%), Postives = 586/621 (94.36%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLLENCSNMKQLK+IHAQMI+T T  +P+LATKLLTLCTSPH
Sbjct: 1   MFALKAESPMQSTWAQTMSLLENCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           LQ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LQEFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+Y LSPSIEHYGCMVDLLGRAGLLEEAKELIK MPMKPNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYILSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           RVPIPPGKS ITLNGVVHEFLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RVPIPPGKSSITLNGVVHEFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+E KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+SRIY REII
Sbjct: 541 LENEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR GSCSC+DYW
Sbjct: 601 MRDRVRFHHFRGGSCSCKDYW 621

BLAST of Lag0013415 vs. NCBI nr
Match: XP_022949774.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita moschata])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLLENCSNMKQLK+IHAQMI+T T  +P+LATKLLTLC SPH
Sbjct: 1   MFALKAESPVQSTWAQTMSLLENCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCISPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LREFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+YNLSPSIEHYGCMVDLLGRAGLLEEAKELIK MPM+PNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           R+PIPPGKS ITLNGVVHEFLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RLPIPPGKSSITLNGVVHEFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LESE KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+S+IY REII
Sbjct: 541 LESEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR G+CSC DYW
Sbjct: 601 MRDRVRFHHFRGGNCSCNDYW 621

BLAST of Lag0013415 vs. NCBI nr
Match: XP_023543056.1 (pentatricopeptide repeat-containing protein At5g66520 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 541/621 (87.12%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLL+NCSNMKQLK+IHAQMI+T T  +P+LATKLLTLCTSPH
Sbjct: 1   MFALKAESPMQSTWAQTMSLLDNCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
            GDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYR+MLSSSVPHNSYTFPFL
Sbjct: 61  LGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRRMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LREFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+YNLSPSIEHYGCMVDLLGRAGLLEEAKELIK MPMKPNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           R+PIPPGKS ITLNGVVH+FLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RLPIPPGKSSITLNGVVHQFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+E KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+SRIY REII
Sbjct: 541 LENEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR G+CSC DYW
Sbjct: 601 MRDRVRFHHFRGGNCSCNDYW 621

BLAST of Lag0013415 vs. NCBI nr
Match: KAG6603968.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 542/621 (87.28%), Postives = 584/621 (94.04%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLLENCSNMKQLK+IHAQMI+T T  +P+LATKLLTLC SPH
Sbjct: 1   MFALKAESPVQSTWAQTMSLLENCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCISPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           G ELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GCELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LREFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+YNLSPSIEHYGCMVDLLGRAGLLEEAKELIK MPM+PNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           R+PIPPGKS ITLNGVVHEFLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RLPIPPGKSSITLNGVVHEFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LESE KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+S+IY REII
Sbjct: 541 LESEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR G+CSC DYW
Sbjct: 601 MRDRVRFHHFRGGNCSCNDYW 621

BLAST of Lag0013415 vs. NCBI nr
Match: XP_038882528.1 (pentatricopeptide repeat-containing protein At5g66520 [Benincasa hispida])

HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 535/621 (86.15%), Postives = 578/621 (93.08%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M TL A  PL+STWAQT+SLLENCSNMKQLK+IHAQMIKTET  +P+LATKLLTLCTSPH
Sbjct: 1   MFTLKADSPLQSTWAQTMSLLENCSNMKQLKEIHAQMIKTETATEPKLATKLLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDL YAQ++FNGIT P TFMWNAIIRAYSNS EPELAFLLY+QMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLPYAQRVFNGITRPNTFMWNAIIRAYSNSKEPELAFLLYQQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRNLSAMGEALQ+HGL++KLGFGSDVFALN+LL+VYALCGDI YARQLFDNIP RDV
Sbjct: 121 LKACRNLSAMGEALQIHGLVIKLGFGSDVFALNALLHVYALCGDIYYARQLFDNIPVRDV 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAG +VEAL+LCYEMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGQSVEALSLCYEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDG+ IASLLTACANLGALDQGRWLHFYVLNNGV +D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGIAIASLLTACANLGALDQGRWLHFYVLNNGVDVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+VFGKLK DQKDVYVWTAMIDGFAIHG GVEALEWF+RM +EGIRPNSITFTA+L ACS
Sbjct: 301 LRVFGKLKSDQKDVYVWTAMIDGFAIHGLGVEALEWFNRMQREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLV EGK LF SM SLYNL PSIEHYGCMVDLLGRAGLL+EAKELIKKMPMKPNAVIW
Sbjct: 361 YAGLVGEGKELFESMTSLYNLIPSIEHYGCMVDLLGRAGLLDEAKELIKKMPMKPNAVIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKAC IH +FL+GSQIGAHLVEVDSDHSGRYI LA+I AAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACWIHRDFLVGSQIGAHLVEVDSDHSGRYIQLATIFAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
            V IPPGKS IT+NGVVHEFLAG QDHPQME+IH KL Q+AERLR++EGY P TKDLLLD
Sbjct: 481 GVSIPPGKSSITVNGVVHEFLAGQQDHPQMEKIHLKLKQIAERLRRDEGYEPSTKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+EEKETAMAQHSEKLAIAFGLINTKPG TIRV+KNLRVCEDCH VAKL+S+IY R II
Sbjct: 541 LENEEKETAMAQHSEKLAIAFGLINTKPGMTIRVIKNLRVCEDCHVVAKLISQIYCRGII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR+G+CSC+DYW
Sbjct: 601 MRDRVRFHHFRNGNCSCKDYW 621

BLAST of Lag0013415 vs. ExPASy Swiss-Prot
Match: Q9FJY7 (Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H61 PE=2 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 2.8e-202
Identity = 345/623 (55.38%), Postives = 449/623 (72.07%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  ++  F LE    +T+S L+ CS  ++LKQIHA+M+KT  + D    TK L+ C S  
Sbjct: 1   MNVISCSFSLEHNLYETMSCLQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISST 60

Query: 61  FGD-LHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPF 120
             D L YAQ +F+G   P TF+WN +IR +S S+EPE + LLY++ML SS PHN+YTFP 
Sbjct: 61  SSDFLPYAQIVFDGFDRPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPS 120

Query: 121 LLKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERD 180
           LLKAC NLSA  E  Q+H  + KLG+ +DV+A+NSL+  YA+ G+ + A  LFD IPE D
Sbjct: 121 LLKACSNLSAFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPD 180

Query: 181 VVSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQR 240
            VSWN++I GY+K G ++ A  +F  M  KN +SWT+MISG V+A +N EAL L +EMQ 
Sbjct: 181 DVSWNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQN 240

Query: 241 AGFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEE 300
           +  E D V +A+ L+ACA LGAL+QG+W+H Y+    + +D V+GC L++MY KCG+MEE
Sbjct: 241 SDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEE 300

Query: 301 ALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTAC 360
           AL+VF  +K  +K V  WTA+I G+A HG G EA+  F  M K GI+PN ITFTA+LTAC
Sbjct: 301 ALEVFKNIK--KKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTAC 360

Query: 361 SYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVI 420
           SY GLVEEGK++F SM+  YNL P+IEHYGC+VDLLGRAGLL+EAK  I++MP+KPNAVI
Sbjct: 361 SYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVI 420

Query: 421 WGALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKN 480
           WGALLKACRIH N  LG +IG  L+ +D  H GRY+H A+I A + KW +AAE R  MK 
Sbjct: 421 WGALLKACRIHKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKE 480

Query: 481 LRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLL 540
             V   PG S I+L G  HEFLAG + HP++E+I  K  ++  R  +E GYVP+ +++LL
Sbjct: 481 QGVAKVPGCSTISLEGTTHEFLAGDRSHPEIEKIQSKW-RIMRRKLEENGYVPELEEMLL 540

Query: 541 DL-ESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGRE 600
           DL + +E+E  + QHSEKLAI +GLI TKPGT IR++KNLRVC+DCH V KL+S+IY R+
Sbjct: 541 DLVDDDEREAIVHQHSEKLAITYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRD 600

Query: 601 IIMRDRVRFHHFRDGSCSCRDYW 622
           I+MRDR RFHHFRDG CSC DYW
Sbjct: 601 IVMRDRTRFHHFRDGKCSCGDYW 620

BLAST of Lag0013415 vs. ExPASy Swiss-Prot
Match: Q9FI80 (Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H38 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.9e-157
Identity = 278/620 (44.84%), Postives = 407/620 (65.65%), Query Frame = 0

Query: 21  LENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSP--HFGDLHYAQKIFNGITSPT 80
           + NC  ++ L QIHA  IK+  + D   A ++L  C +   H  DL YA KIFN +    
Sbjct: 30  INNCRTIRDLSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLDYAHKIFNQMPQRN 89

Query: 81  TFMWNAIIRAYSNSNEPE--LAFLLYRQMLSSS-VPHNSYTFPFLLKACRNLSAMGEALQ 140
            F WN IIR +S S+E +  +A  L+ +M+S   V  N +TFP +LKAC     + E  Q
Sbjct: 90  CFSWNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQ 149

Query: 141 VHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLF-DNIPERD-------------VV 200
           +HGL +K GFG D F +++L+ +Y +CG ++ AR LF  NI E+D             +V
Sbjct: 150 IHGLALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIV 209

Query: 201 SWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRAG 260
            WN MIDGY++ GD + A  +F  M  +++VSW +MISG    G   +A+ +  EM++  
Sbjct: 210 LWNVMIDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNGFFKDAVEVFREMKKGD 269

Query: 261 FELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEAL 320
              + V + S+L A + LG+L+ G WLH Y  ++G+ ID V+G AL++MY KCG +E+A+
Sbjct: 270 IRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALIDMYSKCGIIEKAI 329

Query: 321 QVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACSY 380
            VF +L   +++V  W+AMI+GFAIHG+  +A++ F +M + G+RP+ + +  +LTACS+
Sbjct: 330 HVFERL--PRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLLTACSH 389

Query: 381 AGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIWG 440
            GLVEEG+  F  M S+  L P IEHYGCMVDLLGR+GLL+EA+E I  MP+KP+ VIW 
Sbjct: 390 GGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPDDVIWK 449

Query: 441 ALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNLR 500
           ALL ACR+ GN  +G ++   L+++    SG Y+ L+++ A++G W E +E+RL+MK   
Sbjct: 450 ALLGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEVSEMRLRMKEKD 509

Query: 501 VPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLDL 560
           +   PG SLI ++GV+HEF+     HP+ ++I+  L +++++LR   GY P T  +LL+L
Sbjct: 510 IRKDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRL-AGYRPITTQVLLNL 569

Query: 561 ESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREIIM 620
           E E+KE  +  HSEK+A AFGLI+T PG  IR+VKNLR+CEDCH+  KL+S++Y R+I +
Sbjct: 570 EEEDKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLISKVYKRKITV 629

Query: 621 RDRVRFHHFRDGSCSCRDYW 622
           RDR RFHHF+DGSCSC DYW
Sbjct: 630 RDRKRFHHFQDGSCSCMDYW 646

BLAST of Lag0013415 vs. ExPASy Swiss-Prot
Match: Q9FG16 (Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H88 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 5.5e-150
Identity = 267/610 (43.77%), Postives = 403/610 (66.07%), Query Frame = 0

Query: 18  ISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPHFGD-----LHYAQKIFN 77
           ++LL++CS+   LK IH  +++T  I+D  +A++LL LC      +     L YA  IF+
Sbjct: 16  LALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGIFS 75

Query: 78  GITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNLSAMGE 137
            I +P  F++N +IR +S   EP  AF  Y QML S +  ++ TFPFL+KA   +  +  
Sbjct: 76  QIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLV 135

Query: 138 ALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDVVSWNTMIDGYIK 197
             Q H  +++ GF +DV+  NSL+++YA CG I  A ++F  +  RDVVSW +M+ GY K
Sbjct: 136 GEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGYCK 195

Query: 198 FGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRAGFELDGVVIASL 257
            G VE A  +F +MP +NL +W+ MI+G  +     +A++L   M+R G   +  V+ S+
Sbjct: 196 CGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMVSV 255

Query: 258 LTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQK 317
           +++CA+LGAL+ G   + YV+ + + ++ ++G ALV+M+ +CGD+E+A+ VF  L   + 
Sbjct: 256 ISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGL--PET 315

Query: 318 DVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLF 377
           D   W+++I G A+HG   +A+ +F +M+  G  P  +TFTA+L+ACS+ GLVE+G  ++
Sbjct: 316 DSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIY 375

Query: 378 RSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGN 437
            +MK  + + P +EHYGC+VD+LGRAG L EA+  I KM +KPNA I GALL AC+I+ N
Sbjct: 376 ENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKN 435

Query: 438 FLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLIT 497
             +  ++G  L++V  +HSG Y+ L++I A  G+W +   +R  MK   V  PPG SLI 
Sbjct: 436 TEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIE 495

Query: 498 LNGVVHEFLAG-HQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLDLESEEKETAMA 557
           ++G +++F  G  Q HP+M +I +K  ++  ++R   GY   T D   D++ EEKE+++ 
Sbjct: 496 IDGKINKFTMGDDQKHPEMGKIRRKWEEILGKIRL-IGYKGNTGDAFFDVDEEEKESSIH 555

Query: 558 QHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREIIMRDRVRFHHFR 617
            HSEKLAIA+G++ TKPGTTIR+VKNLRVCEDCHTV KL+S +YGRE+I+RDR RFHHFR
Sbjct: 556 MHSEKLAIAYGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFR 615

Query: 618 DGSCSCRDYW 622
           +G CSCRDYW
Sbjct: 616 NGVCSCRDYW 622

BLAST of Lag0013415 vs. ExPASy Swiss-Prot
Match: O82380 (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.8e-146
Identity = 269/708 (37.99%), Postives = 409/708 (57.77%), Query Frame = 0

Query: 18  ISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPHFGDLHYAQKIFNGITSP 77
           ISL+E C +++QLKQ H  MI+T T +DP  A+KL  +     F  L YA+K+F+ I  P
Sbjct: 34  ISLIERCVSLRQLKQTHGHMIRTGTFSDPYSASKLFAMAALSSFASLEYARKVFDEIPKP 93

Query: 78  TTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPH-NSYTFPFLLKACRNLSAMGEALQV 137
            +F WN +IRAY++  +P L+   +  M+S S  + N YTFPFL+KA   +S++     +
Sbjct: 94  NSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKAAAEVSSLSLGQSL 153

Query: 138 HGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDVVSWNTMIDGYIKFG-- 197
           HG+ +K   GSDVF  NSL++ Y  CGD+  A ++F  I E+DVVSWN+MI+G+++ G  
Sbjct: 154 HGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSP 213

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 214 DKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAM 273

Query: 258 ---------------------------------------DVETAYGVFLDMPMKNLVSWT 317
                                                  D E A  V   MP K++V+W 
Sbjct: 274 LDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWN 333

Query: 318 SMISGIVEAGLNVEALNLCYEMQ-RAGFELDGVVIASLLTACANLGALDQGRWLHFYVLN 377
           ++IS   + G   EAL + +E+Q +   +L+ + + S L+ACA +GAL+ GRW+H Y+  
Sbjct: 334 ALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKK 393

Query: 378 NGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEAL 437
           +G+ ++  +  AL++MY KCGD+E++ +VF  +  +++DV+VW+AMI G A+HG G EA+
Sbjct: 394 HGIRMNFHVTSALIHMYSKCGDLEKSREVFNSV--EKRDVFVWSAMIGGLAMHGCGNEAV 453

Query: 438 EWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDL 497
           + F +M +  ++PN +TFT +  ACS+ GLV+E + LF  M+S Y + P  +HY C+VD+
Sbjct: 454 DMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYACIVDV 513

Query: 498 LGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRY 557
           LGR+G LE+A + I+ MP+ P+  +WGALL AC+IH N  L       L+E++  + G +
Sbjct: 514 LGRSGYLEKAVKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRNDGAH 573

Query: 558 IHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIH 617
           + L++I A  GKW+  +E+R  M+   +   PG S I ++G++HEFL+G   HP  E+++
Sbjct: 574 VLLSNIYAKLGKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVY 633

Query: 618 QKLNQVAERLRQEEGYVPKTKDLLLDLESEE-KETAMAQHSEKLAIAFGLINTKPGTTIR 622
            KL++V E+L+   GY P+   +L  +E EE KE ++  HSEKLAI +GLI+T+    IR
Sbjct: 634 GKLHEVMEKLK-SNGYEPEISQVLQIIEEEEMKEQSLNLHSEKLAICYGLISTEAPKVIR 693

BLAST of Lag0013415 vs. ExPASy Swiss-Prot
Match: Q9LN01 (Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H12 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 8.2e-146
Identity = 283/709 (39.92%), Postives = 402/709 (56.70%), Query Frame = 0

Query: 17  TISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLC-TSPHFGDLHYAQKIFNGIT 76
           ++SLL NC  ++ L+ IHAQMIK          +KL+  C  SPHF  L YA  +F  I 
Sbjct: 36  SLSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQ 95

Query: 77  SPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNLSAMGEALQ 136
            P   +WN + R ++ S++P  A  LY  M+S  +  NSYTFPF+LK+C    A  E  Q
Sbjct: 96  EPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 155

Query: 137 VHGLMMKLGFGSDVFALNSL--LYV-----------------------------YALCGD 196
           +HG ++KLG   D++   SL  +YV                             YA  G 
Sbjct: 156 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 215

Query: 197 IQYARQLFDNIPERDVVSWNTMIDGYI--------------------------------- 256
           I+ A++LFD IP +DVVSWN MI GY                                  
Sbjct: 216 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 275

Query: 257 -------------------------------------KFGDVETAYGVFLDMPMKNLVSW 316
                                                K G++ETA G+F  +P K+++SW
Sbjct: 276 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 335

Query: 317 TSMISGIVEAGLNVEALNLCYEMQRAGFELDGVVIASLLTACANLGALDQGRWLHFYVLN 376
            ++I G     L  EAL L  EM R+G   + V + S+L ACA+LGA+D GRW+H Y+  
Sbjct: 336 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 395

Query: 377 --NGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVE 436
              GV     +  +L++MY KCGD+E A QVF  +    K +  W AMI GFA+HGR   
Sbjct: 396 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSIL--HKSLSSWNAMIFGFAMHGRADA 455

Query: 437 ALEWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMV 496
           + + F RM K GI+P+ ITF  +L+ACS++G+++ G+ +FR+M   Y ++P +EHYGCM+
Sbjct: 456 SFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 515

Query: 497 DLLGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGNFLLGSQIGAHLVEVDSDHSG 556
           DLLG +GL +EA+E+I  M M+P+ VIW +LLKAC++HGN  LG     +L++++ ++ G
Sbjct: 516 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 575

Query: 557 RYIHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQ 616
            Y+ L++I A+ G+W E A+ R  + +  +   PG S I ++ VVHEF+ G + HP+  +
Sbjct: 576 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 635

Query: 617 IHQKLNQVAERLRQEEGYVPKTKDLLLDLESEEKETAMAQHSEKLAIAFGLINTKPGTTI 622
           I+  L ++ E L ++ G+VP T ++L ++E E KE A+  HSEKLAIAFGLI+TKPGT +
Sbjct: 636 IYGMLEEM-EVLLEKAGFVPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKL 695

BLAST of Lag0013415 vs. ExPASy TrEMBL
Match: A0A6J1IT43 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita maxima OX=3661 GN=LOC111478422 PE=3 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 547/621 (88.08%), Postives = 586/621 (94.36%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLLENCSNMKQLK+IHAQMI+T T  +P+LATKLLTLCTSPH
Sbjct: 1   MFALKAESPMQSTWAQTMSLLENCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           LQ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LQEFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+Y LSPSIEHYGCMVDLLGRAGLLEEAKELIK MPMKPNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYILSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMKPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           RVPIPPGKS ITLNGVVHEFLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RVPIPPGKSSITLNGVVHEFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+E KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+SRIY REII
Sbjct: 541 LENEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISRIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR GSCSC+DYW
Sbjct: 601 MRDRVRFHHFRGGSCSCKDYW 621

BLAST of Lag0013415 vs. ExPASy TrEMBL
Match: A0A6J1GDX2 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita moschata OX=3662 GN=LOC111453066 PE=3 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 543/621 (87.44%), Postives = 585/621 (94.20%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  L A+ P++STWAQT+SLLENCSNMKQLK+IHAQMI+T T  +P+LATKLLTLC SPH
Sbjct: 1   MFALKAESPVQSTWAQTMSLLENCSNMKQLKEIHAQMIRTGTATEPKLATKLLTLCISPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
           FGDLHYAQ++FNGI+SPTTFMWNA+IRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL
Sbjct: 61  FGDLHYAQRVFNGISSPTTFMWNAMIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRN SAM EALQVHGL++KLGFGSDVFALN+LL+VYALCGDIQYARQLFDNIPERD+
Sbjct: 121 LKACRNFSAMSEALQVHGLVIKLGFGSDVFALNALLHVYALCGDIQYARQLFDNIPERDI 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAGLNVEAL+LC+EMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGLNVEALSLCHEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGVH+D+VIGCALVNMYLKCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVHVDRVIGCALVNMYLKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+ FGKLK DQKDVYVWTAMIDGFAIHGRGVEALEWF RM++EGIRPNSITFTA+L ACS
Sbjct: 301 LREFGKLKGDQKDVYVWTAMIDGFAIHGRGVEALEWFKRMLREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           YAGLVEEGKVLF SM S+YNLSPSIEHYGCMVDLLGRAGLLEEAKELIK MPM+PNA+IW
Sbjct: 361 YAGLVEEGKVLFESMMSVYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKTMPMEPNAIIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKACRIH +FL+G QIGAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMKNL
Sbjct: 421 GALLKACRIHRDFLVGGQIGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
           R+PIPPGKS ITLNGVVHEFLAGHQDHPQMEQI  KLNQV ERLRQ EGY P TKDLLLD
Sbjct: 481 RLPIPPGKSSITLNGVVHEFLAGHQDHPQMEQILHKLNQVVERLRQHEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LESE KETA+AQHSEKLAIAFGLINTKPG+TIRVVKNLRVCEDCH VAKL+S+IY REII
Sbjct: 541 LESEAKETAVAQHSEKLAIAFGLINTKPGSTIRVVKNLRVCEDCHVVAKLISQIYRREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFR G+CSC DYW
Sbjct: 601 MRDRVRFHHFRGGNCSCNDYW 621

BLAST of Lag0013415 vs. ExPASy TrEMBL
Match: A0A0A0KKE0 (DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502750 PE=3 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 525/621 (84.54%), Postives = 573/621 (92.27%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M TL A+ PL+STWA    LLENCSNMKQLKQI AQMIKT  I +P+LATK LTLCTSPH
Sbjct: 1   MFTLNAESPLQSTWA----LLENCSNMKQLKQIQAQMIKTAIITEPKLATKFLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
            GDL YAQ++FNGITSP TFMWNAIIRAYSNS+EPELAFL Y+QMLSSSVPHNSYTFPFL
Sbjct: 61  VGDLLYAQRVFNGITSPNTFMWNAIIRAYSNSDEPELAFLSYQQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           L+ACRNL AMGEALQVHGL++KLGFGSDVFALN+LL+VYALCG+I  ARQLFDNIPERD 
Sbjct: 121 LRACRNLLAMGEALQVHGLVIKLGFGSDVFALNALLHVYALCGEIHCARQLFDNIPERDA 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYGVFLDMP+KN+VSWTS+ISG+VEAG +VEAL+LCYEMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGVFLDMPLKNVVSWTSLISGLVEAGQSVEALSLCYEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IASLLTACANLGALDQGRWLHFYVLNNGV +D+VIGCALVNMY+KCGDMEEA
Sbjct: 241 GFELDGVAIASLLTACANLGALDQGRWLHFYVLNNGVDVDRVIGCALVNMYVKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L VFGKLK +QKDVY+WTAMIDGFAIHGRGVEALEWF+RM +EGIRPNSITFTA+L ACS
Sbjct: 301 LSVFGKLKGNQKDVYIWTAMIDGFAIHGRGVEALEWFNRMRREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           Y GLVEEGK LF+SMK  YN++PSIEHYGCMVDLLGR+G L+EAKELIKKMPMKP+AVIW
Sbjct: 361 YGGLVEEGKELFKSMKCFYNVNPSIEHYGCMVDLLGRSGRLDEAKELIKKMPMKPSAVIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GALLKAC IH +FLLGSQ+GAHLVEVDSDHSGRYI LA+ILAAEGKWKEAAEVRLKMK+L
Sbjct: 421 GALLKACWIHRDFLLGSQVGAHLVEVDSDHSGRYIQLATILAAEGKWKEAAEVRLKMKSL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
            VPI PGKS +TLNG+VHEFLAGHQDHPQMEQI  KL Q+AERLRQ+EGY P TKDLLLD
Sbjct: 481 GVPISPGKSSVTLNGIVHEFLAGHQDHPQMEQIQLKLKQIAERLRQDEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+EEKETAMAQHSEKLAIAFGLINTKPGTTIRV+KNLR+C DCHTVAKLVS+IY REII
Sbjct: 541 LENEEKETAMAQHSEKLAIAFGLINTKPGTTIRVIKNLRICRDCHTVAKLVSQIYSREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFRDGSCSC+DYW
Sbjct: 601 MRDRVRFHHFRDGSCSCKDYW 617

BLAST of Lag0013415 vs. ExPASy TrEMBL
Match: A0A5D3CKZ8 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002030 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 525/621 (84.54%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M TL A+ PL+STW    +LLENCSNMKQLKQI AQMIKT  +++P+LATK LTLCTSPH
Sbjct: 1   MFTLKAESPLQSTW----TLLENCSNMKQLKQIQAQMIKTAILSEPKLATKFLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
            GDL YAQ++FNGITSP T MWNAIIRAYSNS EPELAFLLY+QMLSSSVPHNSYTFPFL
Sbjct: 61  VGDLLYAQRVFNGITSPNTVMWNAIIRAYSNSKEPELAFLLYQQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRNLSA+GEALQVHGL++KLGFGSDVFALN+LL+VYALCG+I+YARQ+FDNIPERD 
Sbjct: 121 LKACRNLSALGEALQVHGLVIKLGFGSDVFALNALLHVYALCGEIRYARQMFDNIPERDA 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYG+FLDMP KN+VSWTS+ISG+V AGL+V+AL+LCYEMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGIFLDMPSKNVVSWTSLISGLVGAGLSVKALSLCYEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IA LLTACANLGALDQGRWLHFYVLNNGV +D+VIGCALVNMY+KCGDMEEA
Sbjct: 241 GFELDGVAIACLLTACANLGALDQGRWLHFYVLNNGVDVDRVIGCALVNMYVKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+VFGKLK DQKDV +WTAMIDGFAIHGRGVEALEWFD M +EGIRPNSITFTA+L ACS
Sbjct: 301 LRVFGKLKGDQKDVCIWTAMIDGFAIHGRGVEALEWFDLMRREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           Y GLVEEGK LF+SMK LYNLSPSIEHYGCMVDLLGR+G L EAKELIK MPMKPNAVIW
Sbjct: 361 YGGLVEEGKELFKSMKCLYNLSPSIEHYGCMVDLLGRSGRLNEAKELIKNMPMKPNAVIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GA LKAC IH +FL+GSQIGAHLVEVDSDHSGRYI LA+ILAA+GKWKEAAEVRLKMKNL
Sbjct: 421 GAFLKACWIHRDFLVGSQIGAHLVEVDSDHSGRYIQLATILAAQGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
            VPI PGKS ITLNG+VHEFLAGHQDHPQMEQIH KL Q+AERLRQ+EGY P TKDLLLD
Sbjct: 481 GVPISPGKSSITLNGIVHEFLAGHQDHPQMEQIHLKLKQIAERLRQDEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+EEKETA+AQHSEKLAIAFGLINTKPGTTIRVVKNLR+C DCHTVAKLVS+IY REII
Sbjct: 541 LENEEKETAIAQHSEKLAIAFGLINTKPGTTIRVVKNLRICRDCHTVAKLVSQIYCREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFRDGSCSC+DYW
Sbjct: 601 MRDRVRFHHFRDGSCSCKDYW 617

BLAST of Lag0013415 vs. ExPASy TrEMBL
Match: A0A1S3B1S8 (pentatricopeptide repeat-containing protein At5g66520 OS=Cucumis melo OX=3656 GN=LOC103485057 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 525/621 (84.54%), Postives = 572/621 (92.11%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M TL A+ PL+STW    +LLENCSNMKQLKQI AQMIKT  +++P+LATK LTLCTSPH
Sbjct: 1   MFTLKAESPLQSTW----TLLENCSNMKQLKQIQAQMIKTAILSEPKLATKFLTLCTSPH 60

Query: 61  FGDLHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFL 120
            GDL YAQ++FNGITSP T MWNAIIRAYSNS EPELAFLLY+QMLSSSVPHNSYTFPFL
Sbjct: 61  VGDLLYAQRVFNGITSPNTVMWNAIIRAYSNSKEPELAFLLYQQMLSSSVPHNSYTFPFL 120

Query: 121 LKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDV 180
           LKACRNLSA+GEALQVHGL++KLGFGSDVFALN+LL+VYALCG+I+YARQ+FDNIPERD 
Sbjct: 121 LKACRNLSALGEALQVHGLVIKLGFGSDVFALNALLHVYALCGEIRYARQMFDNIPERDA 180

Query: 181 VSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRA 240
           VSWN MIDGYIK GDV+TAYG+FLDMP KN+VSWTS+ISG+V AGL+V+AL+LCYEMQ A
Sbjct: 181 VSWNIMIDGYIKSGDVKTAYGIFLDMPSKNVVSWTSLISGLVGAGLSVKALSLCYEMQNA 240

Query: 241 GFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEA 300
           GFELDGV IA LLTACANLGALDQGRWLHFYVLNNGV +D+VIGCALVNMY+KCGDMEEA
Sbjct: 241 GFELDGVAIACLLTACANLGALDQGRWLHFYVLNNGVDVDRVIGCALVNMYVKCGDMEEA 300

Query: 301 LQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACS 360
           L+VFGKLK DQKDV +WTAMIDGFAIHGRGVEALEWFD M +EGIRPNSITFTA+L ACS
Sbjct: 301 LRVFGKLKGDQKDVCIWTAMIDGFAIHGRGVEALEWFDLMRREGIRPNSITFTAVLRACS 360

Query: 361 YAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIW 420
           Y GLVEEGK LF+SMK LYNLSPSIEHYGCMVDLLGR+G L EAKELIK MPMKPNAVIW
Sbjct: 361 YGGLVEEGKELFKSMKCLYNLSPSIEHYGCMVDLLGRSGRLNEAKELIKNMPMKPNAVIW 420

Query: 421 GALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNL 480
           GA LKAC IH +FL+GSQIGAHLVEVDSDHSGRYI LA+ILAA+GKWKEAAEVRLKMKNL
Sbjct: 421 GAFLKACWIHRDFLVGSQIGAHLVEVDSDHSGRYIQLATILAAQGKWKEAAEVRLKMKNL 480

Query: 481 RVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLD 540
            VPI PGKS ITLNG+VHEFLAGHQDHPQMEQIH KL Q+AERLRQ+EGY P TKDLLLD
Sbjct: 481 GVPISPGKSSITLNGIVHEFLAGHQDHPQMEQIHLKLKQIAERLRQDEGYEPATKDLLLD 540

Query: 541 LESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREII 600
           LE+EEKETA+AQHSEKLAIAFGLINTKPGTTIRVVKNLR+C DCHTVAKLVS+IY REII
Sbjct: 541 LENEEKETAIAQHSEKLAIAFGLINTKPGTTIRVVKNLRICRDCHTVAKLVSQIYCREII 600

Query: 601 MRDRVRFHHFRDGSCSCRDYW 622
           MRDRVRFHHFRDGSCSC+DYW
Sbjct: 601 MRDRVRFHHFRDGSCSCKDYW 617

BLAST of Lag0013415 vs. TAIR 10
Match: AT5G66520.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 706.4 bits (1822), Expect = 2.0e-203
Identity = 345/623 (55.38%), Postives = 449/623 (72.07%), Query Frame = 0

Query: 1   MITLTAQFPLESTWAQTISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPH 60
           M  ++  F LE    +T+S L+ CS  ++LKQIHA+M+KT  + D    TK L+ C S  
Sbjct: 1   MNVISCSFSLEHNLYETMSCLQRCSKQEELKQIHARMLKTGLMQDSYAITKFLSFCISST 60

Query: 61  FGD-LHYAQKIFNGITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPF 120
             D L YAQ +F+G   P TF+WN +IR +S S+EPE + LLY++ML SS PHN+YTFP 
Sbjct: 61  SSDFLPYAQIVFDGFDRPDTFLWNLMIRGFSCSDEPERSLLLYQRMLCSSAPHNAYTFPS 120

Query: 121 LLKACRNLSAMGEALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERD 180
           LLKAC NLSA  E  Q+H  + KLG+ +DV+A+NSL+  YA+ G+ + A  LFD IPE D
Sbjct: 121 LLKACSNLSAFEETTQIHAQITKLGYENDVYAVNSLINSYAVTGNFKLAHLLFDRIPEPD 180

Query: 181 VVSWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQR 240
            VSWN++I GY+K G ++ A  +F  M  KN +SWT+MISG V+A +N EAL L +EMQ 
Sbjct: 181 DVSWNSVIKGYVKAGKMDIALTLFRKMAEKNAISWTTMISGYVQADMNKEALQLFHEMQN 240

Query: 241 AGFELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEE 300
           +  E D V +A+ L+ACA LGAL+QG+W+H Y+    + +D V+GC L++MY KCG+MEE
Sbjct: 241 SDVEPDNVSLANALSACAQLGALEQGKWIHSYLNKTRIRMDSVLGCVLIDMYAKCGEMEE 300

Query: 301 ALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTAC 360
           AL+VF  +K  +K V  WTA+I G+A HG G EA+  F  M K GI+PN ITFTA+LTAC
Sbjct: 301 ALEVFKNIK--KKSVQAWTALISGYAYHGHGREAISKFMEMQKMGIKPNVITFTAVLTAC 360

Query: 361 SYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVI 420
           SY GLVEEGK++F SM+  YNL P+IEHYGC+VDLLGRAGLL+EAK  I++MP+KPNAVI
Sbjct: 361 SYTGLVEEGKLIFYSMERDYNLKPTIEHYGCIVDLLGRAGLLDEAKRFIQEMPLKPNAVI 420

Query: 421 WGALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKN 480
           WGALLKACRIH N  LG +IG  L+ +D  H GRY+H A+I A + KW +AAE R  MK 
Sbjct: 421 WGALLKACRIHKNIELGEEIGEILIAIDPYHGGRYVHKANIHAMDKKWDKAAETRRLMKE 480

Query: 481 LRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLL 540
             V   PG S I+L G  HEFLAG + HP++E+I  K  ++  R  +E GYVP+ +++LL
Sbjct: 481 QGVAKVPGCSTISLEGTTHEFLAGDRSHPEIEKIQSKW-RIMRRKLEENGYVPELEEMLL 540

Query: 541 DL-ESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGRE 600
           DL + +E+E  + QHSEKLAI +GLI TKPGT IR++KNLRVC+DCH V KL+S+IY R+
Sbjct: 541 DLVDDDEREAIVHQHSEKLAITYGLIKTKPGTIIRIMKNLRVCKDCHKVTKLISKIYKRD 600

Query: 601 IIMRDRVRFHHFRDGSCSCRDYW 622
           I+MRDR RFHHFRDG CSC DYW
Sbjct: 601 IVMRDRTRFHHFRDGKCSCGDYW 620

BLAST of Lag0013415 vs. TAIR 10
Match: AT5G48910.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.6e-158
Identity = 278/620 (44.84%), Postives = 407/620 (65.65%), Query Frame = 0

Query: 21  LENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSP--HFGDLHYAQKIFNGITSPT 80
           + NC  ++ L QIHA  IK+  + D   A ++L  C +   H  DL YA KIFN +    
Sbjct: 30  INNCRTIRDLSQIHAVFIKSGQMRDTLAAAEILRFCATSDLHHRDLDYAHKIFNQMPQRN 89

Query: 81  TFMWNAIIRAYSNSNEPE--LAFLLYRQMLSSS-VPHNSYTFPFLLKACRNLSAMGEALQ 140
            F WN IIR +S S+E +  +A  L+ +M+S   V  N +TFP +LKAC     + E  Q
Sbjct: 90  CFSWNTIIRGFSESDEDKALIAITLFYEMMSDEFVEPNRFTFPSVLKACAKTGKIQEGKQ 149

Query: 141 VHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLF-DNIPERD-------------VV 200
           +HGL +K GFG D F +++L+ +Y +CG ++ AR LF  NI E+D             +V
Sbjct: 150 IHGLALKYGFGGDEFVMSNLVRMYVMCGFMKDARVLFYKNIIEKDMVVMTDRRKRDGEIV 209

Query: 201 SWNTMIDGYIKFGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRAG 260
            WN MIDGY++ GD + A  +F  M  +++VSW +MISG    G   +A+ +  EM++  
Sbjct: 210 LWNVMIDGYMRLGDCKAARMLFDKMRQRSVVSWNTMISGYSLNGFFKDAVEVFREMKKGD 269

Query: 261 FELDGVVIASLLTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEAL 320
              + V + S+L A + LG+L+ G WLH Y  ++G+ ID V+G AL++MY KCG +E+A+
Sbjct: 270 IRPNYVTLVSVLPAISRLGSLELGEWLHLYAEDSGIRIDDVLGSALIDMYSKCGIIEKAI 329

Query: 321 QVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACSY 380
            VF +L   +++V  W+AMI+GFAIHG+  +A++ F +M + G+RP+ + +  +LTACS+
Sbjct: 330 HVFERL--PRENVITWSAMINGFAIHGQAGDAIDCFCKMRQAGVRPSDVAYINLLTACSH 389

Query: 381 AGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIWG 440
            GLVEEG+  F  M S+  L P IEHYGCMVDLLGR+GLL+EA+E I  MP+KP+ VIW 
Sbjct: 390 GGLVEEGRRYFSQMVSVDGLEPRIEHYGCMVDLLGRSGLLDEAEEFILNMPIKPDDVIWK 449

Query: 441 ALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNLR 500
           ALL ACR+ GN  +G ++   L+++    SG Y+ L+++ A++G W E +E+RL+MK   
Sbjct: 450 ALLGACRMQGNVEMGKRVANILMDMVPHDSGAYVALSNMYASQGNWSEVSEMRLRMKEKD 509

Query: 501 VPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLDL 560
           +   PG SLI ++GV+HEF+     HP+ ++I+  L +++++LR   GY P T  +LL+L
Sbjct: 510 IRKDPGCSLIDIDGVLHEFVVEDDSHPKAKEINSMLVEISDKLRL-AGYRPITTQVLLNL 569

Query: 561 ESEEKETAMAQHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREIIM 620
           E E+KE  +  HSEK+A AFGLI+T PG  IR+VKNLR+CEDCH+  KL+S++Y R+I +
Sbjct: 570 EEEDKENVLHYHSEKIATAFGLISTSPGKPIRIVKNLRICEDCHSSIKLISKVYKRKITV 629

Query: 621 RDRVRFHHFRDGSCSCRDYW 622
           RDR RFHHF+DGSCSC DYW
Sbjct: 630 RDRKRFHHFQDGSCSCMDYW 646

BLAST of Lag0013415 vs. TAIR 10
Match: AT5G06540.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.9e-151
Identity = 267/610 (43.77%), Postives = 403/610 (66.07%), Query Frame = 0

Query: 18  ISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPHFGD-----LHYAQKIFN 77
           ++LL++CS+   LK IH  +++T  I+D  +A++LL LC      +     L YA  IF+
Sbjct: 16  LALLQSCSSFSDLKIIHGFLLRTHLISDVFVASRLLALCVDDSTFNKPTNLLGYAYGIFS 75

Query: 78  GITSPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNLSAMGE 137
            I +P  F++N +IR +S   EP  AF  Y QML S +  ++ TFPFL+KA   +  +  
Sbjct: 76  QIQNPNLFVFNLLIRCFSTGAEPSKAFGFYTQMLKSRIWPDNITFPFLIKASSEMECVLV 135

Query: 138 ALQVHGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDVVSWNTMIDGYIK 197
             Q H  +++ GF +DV+  NSL+++YA CG I  A ++F  +  RDVVSW +M+ GY K
Sbjct: 136 GEQTHSQIVRFGFQNDVYVENSLVHMYANCGFIAAAGRIFGQMGFRDVVSWTSMVAGYCK 195

Query: 198 FGDVETAYGVFLDMPMKNLVSWTSMISGIVEAGLNVEALNLCYEMQRAGFELDGVVIASL 257
            G VE A  +F +MP +NL +W+ MI+G  +     +A++L   M+R G   +  V+ S+
Sbjct: 196 CGMVENAREMFDEMPHRNLFTWSIMINGYAKNNCFEKAIDLFEFMKREGVVANETVMVSV 255

Query: 258 LTACANLGALDQGRWLHFYVLNNGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQK 317
           +++CA+LGAL+ G   + YV+ + + ++ ++G ALV+M+ +CGD+E+A+ VF  L   + 
Sbjct: 256 ISSCAHLGALEFGERAYEYVVKSHMTVNLILGTALVDMFWRCGDIEKAIHVFEGL--PET 315

Query: 318 DVYVWTAMIDGFAIHGRGVEALEWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLF 377
           D   W+++I G A+HG   +A+ +F +M+  G  P  +TFTA+L+ACS+ GLVE+G  ++
Sbjct: 316 DSLSWSSIIKGLAVHGHAHKAMHYFSQMISLGFIPRDVTFTAVLSACSHGGLVEKGLEIY 375

Query: 378 RSMKSLYNLSPSIEHYGCMVDLLGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGN 437
            +MK  + + P +EHYGC+VD+LGRAG L EA+  I KM +KPNA I GALL AC+I+ N
Sbjct: 376 ENMKKDHGIEPRLEHYGCIVDMLGRAGKLAEAENFILKMHVKPNAPILGALLGACKIYKN 435

Query: 438 FLLGSQIGAHLVEVDSDHSGRYIHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLIT 497
             +  ++G  L++V  +HSG Y+ L++I A  G+W +   +R  MK   V  PPG SLI 
Sbjct: 436 TEVAERVGNMLIKVKPEHSGYYVLLSNIYACAGQWDKIESLRDMMKEKLVKKPPGWSLIE 495

Query: 498 LNGVVHEFLAG-HQDHPQMEQIHQKLNQVAERLRQEEGYVPKTKDLLLDLESEEKETAMA 557
           ++G +++F  G  Q HP+M +I +K  ++  ++R   GY   T D   D++ EEKE+++ 
Sbjct: 496 IDGKINKFTMGDDQKHPEMGKIRRKWEEILGKIRL-IGYKGNTGDAFFDVDEEEKESSIH 555

Query: 558 QHSEKLAIAFGLINTKPGTTIRVVKNLRVCEDCHTVAKLVSRIYGREIIMRDRVRFHHFR 617
            HSEKLAIA+G++ TKPGTTIR+VKNLRVCEDCHTV KL+S +YGRE+I+RDR RFHHFR
Sbjct: 556 MHSEKLAIAYGMMKTKPGTTIRIVKNLRVCEDCHTVTKLISEVYGRELIVRDRNRFHHFR 615

Query: 618 DGSCSCRDYW 622
           +G CSCRDYW
Sbjct: 616 NGVCSCRDYW 622

BLAST of Lag0013415 vs. TAIR 10
Match: AT2G29760.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-147
Identity = 269/708 (37.99%), Postives = 409/708 (57.77%), Query Frame = 0

Query: 18  ISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLCTSPHFGDLHYAQKIFNGITSP 77
           ISL+E C +++QLKQ H  MI+T T +DP  A+KL  +     F  L YA+K+F+ I  P
Sbjct: 34  ISLIERCVSLRQLKQTHGHMIRTGTFSDPYSASKLFAMAALSSFASLEYARKVFDEIPKP 93

Query: 78  TTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPH-NSYTFPFLLKACRNLSAMGEALQV 137
            +F WN +IRAY++  +P L+   +  M+S S  + N YTFPFL+KA   +S++     +
Sbjct: 94  NSFAWNTLIRAYASGPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKAAAEVSSLSLGQSL 153

Query: 138 HGLMMKLGFGSDVFALNSLLYVYALCGDIQYARQLFDNIPERDVVSWNTMIDGYIKFG-- 197
           HG+ +K   GSDVF  NSL++ Y  CGD+  A ++F  I E+DVVSWN+MI+G+++ G  
Sbjct: 154 HGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSP 213

Query: 198 ------------------------------------------------------------ 257
                                                                       
Sbjct: 214 DKALELFKKMESEDVKASHVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAM 273

Query: 258 ---------------------------------------DVETAYGVFLDMPMKNLVSWT 317
                                                  D E A  V   MP K++V+W 
Sbjct: 274 LDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWN 333

Query: 318 SMISGIVEAGLNVEALNLCYEMQ-RAGFELDGVVIASLLTACANLGALDQGRWLHFYVLN 377
           ++IS   + G   EAL + +E+Q +   +L+ + + S L+ACA +GAL+ GRW+H Y+  
Sbjct: 334 ALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKK 393

Query: 378 NGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVEAL 437
           +G+ ++  +  AL++MY KCGD+E++ +VF  +  +++DV+VW+AMI G A+HG G EA+
Sbjct: 394 HGIRMNFHVTSALIHMYSKCGDLEKSREVFNSV--EKRDVFVWSAMIGGLAMHGCGNEAV 453

Query: 438 EWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMVDL 497
           + F +M +  ++PN +TFT +  ACS+ GLV+E + LF  M+S Y + P  +HY C+VD+
Sbjct: 454 DMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAESLFHQMESNYGIVPEEKHYACIVDV 513

Query: 498 LGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGNFLLGSQIGAHLVEVDSDHSGRY 557
           LGR+G LE+A + I+ MP+ P+  +WGALL AC+IH N  L       L+E++  + G +
Sbjct: 514 LGRSGYLEKAVKFIEAMPIPPSTSVWGALLGACKIHANLNLAEMACTRLLELEPRNDGAH 573

Query: 558 IHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQIH 617
           + L++I A  GKW+  +E+R  M+   +   PG S I ++G++HEFL+G   HP  E+++
Sbjct: 574 VLLSNIYAKLGKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVY 633

Query: 618 QKLNQVAERLRQEEGYVPKTKDLLLDLESEE-KETAMAQHSEKLAIAFGLINTKPGTTIR 622
            KL++V E+L+   GY P+   +L  +E EE KE ++  HSEKLAI +GLI+T+    IR
Sbjct: 634 GKLHEVMEKLK-SNGYEPEISQVLQIIEEEEMKEQSLNLHSEKLAICYGLISTEAPKVIR 693

BLAST of Lag0013415 vs. TAIR 10
Match: AT1G08070.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 518.8 bits (1335), Expect = 5.8e-147
Identity = 283/709 (39.92%), Postives = 402/709 (56.70%), Query Frame = 0

Query: 17  TISLLENCSNMKQLKQIHAQMIKTETIADPQLATKLLTLC-TSPHFGDLHYAQKIFNGIT 76
           ++SLL NC  ++ L+ IHAQMIK          +KL+  C  SPHF  L YA  +F  I 
Sbjct: 36  SLSLLHNCKTLQSLRIIHAQMIKIGLHNTNYALSKLIEFCILSPHFEGLPYAISVFKTIQ 95

Query: 77  SPTTFMWNAIIRAYSNSNEPELAFLLYRQMLSSSVPHNSYTFPFLLKACRNLSAMGEALQ 136
            P   +WN + R ++ S++P  A  LY  M+S  +  NSYTFPF+LK+C    A  E  Q
Sbjct: 96  EPNLLIWNTMFRGHALSSDPVSALKLYVCMISLGLLPNSYTFPFVLKSCAKSKAFKEGQQ 155

Query: 137 VHGLMMKLGFGSDVFALNSL--LYV-----------------------------YALCGD 196
           +HG ++KLG   D++   SL  +YV                             YA  G 
Sbjct: 156 IHGHVLKLGCDLDLYVHTSLISMYVQNGRLEDAHKVFDKSPHRDVVSYTALIKGYASRGY 215

Query: 197 IQYARQLFDNIPERDVVSWNTMIDGYI--------------------------------- 256
           I+ A++LFD IP +DVVSWN MI GY                                  
Sbjct: 216 IENAQKLFDEIPVKDVVSWNAMISGYAETGNYKEALELFKDMMKTNVRPDESTMVTVVSA 275

Query: 257 -------------------------------------KFGDVETAYGVFLDMPMKNLVSW 316
                                                K G++ETA G+F  +P K+++SW
Sbjct: 276 CAQSGSIELGRQVHLWIDDHGFGSNLKIVNALIDLYSKCGELETACGLFERLPYKDVISW 335

Query: 317 TSMISGIVEAGLNVEALNLCYEMQRAGFELDGVVIASLLTACANLGALDQGRWLHFYVLN 376
            ++I G     L  EAL L  EM R+G   + V + S+L ACA+LGA+D GRW+H Y+  
Sbjct: 336 NTLIGGYTHMNLYKEALLLFQEMLRSGETPNDVTMLSILPACAHLGAIDIGRWIHVYIDK 395

Query: 377 --NGVHIDKVIGCALVNMYLKCGDMEEALQVFGKLKDDQKDVYVWTAMIDGFAIHGRGVE 436
              GV     +  +L++MY KCGD+E A QVF  +    K +  W AMI GFA+HGR   
Sbjct: 396 RLKGVTNASSLRTSLIDMYAKCGDIEAAHQVFNSIL--HKSLSSWNAMIFGFAMHGRADA 455

Query: 437 ALEWFDRMVKEGIRPNSITFTAILTACSYAGLVEEGKVLFRSMKSLYNLSPSIEHYGCMV 496
           + + F RM K GI+P+ ITF  +L+ACS++G+++ G+ +FR+M   Y ++P +EHYGCM+
Sbjct: 456 SFDLFSRMRKIGIQPDDITFVGLLSACSHSGMLDLGRHIFRTMTQDYKMTPKLEHYGCMI 515

Query: 497 DLLGRAGLLEEAKELIKKMPMKPNAVIWGALLKACRIHGNFLLGSQIGAHLVEVDSDHSG 556
           DLLG +GL +EA+E+I  M M+P+ VIW +LLKAC++HGN  LG     +L++++ ++ G
Sbjct: 516 DLLGHSGLFKEAEEMINMMEMEPDGVIWCSLLKACKMHGNVELGESFAENLIKIEPENPG 575

Query: 557 RYIHLASILAAEGKWKEAAEVRLKMKNLRVPIPPGKSLITLNGVVHEFLAGHQDHPQMEQ 616
            Y+ L++I A+ G+W E A+ R  + +  +   PG S I ++ VVHEF+ G + HP+  +
Sbjct: 576 SYVLLSNIYASAGRWNEVAKTRALLNDKGMKKVPGCSSIEIDSVVHEFIIGDKFHPRNRE 635

Query: 617 IHQKLNQVAERLRQEEGYVPKTKDLLLDLESEEKETAMAQHSEKLAIAFGLINTKPGTTI 622
           I+  L ++ E L ++ G+VP T ++L ++E E KE A+  HSEKLAIAFGLI+TKPGT +
Sbjct: 636 IYGMLEEM-EVLLEKAGFVPDTSEVLQEMEEEWKEGALRHHSEKLAIAFGLISTKPGTKL 695

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022978438.10.0e+0088.08pentatricopeptide repeat-containing protein At5g66520 [Cucurbita maxima][more]
XP_022949774.10.0e+0087.44pentatricopeptide repeat-containing protein At5g66520 [Cucurbita moschata][more]
XP_023543056.10.0e+0087.12pentatricopeptide repeat-containing protein At5g66520 [Cucurbita pepo subsp. pep... [more]
KAG6603968.10.0e+0087.28Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_038882528.10.0e+0086.15pentatricopeptide repeat-containing protein At5g66520 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FJY72.8e-20255.38Pentatricopeptide repeat-containing protein At5g66520 OS=Arabidopsis thaliana OX... [more]
Q9FI807.9e-15744.84Pentatricopeptide repeat-containing protein At5g48910 OS=Arabidopsis thaliana OX... [more]
Q9FG165.5e-15043.77Pentatricopeptide repeat-containing protein At5g06540 OS=Arabidopsis thaliana OX... [more]
O823802.8e-14637.99Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
Q9LN018.2e-14639.92Pentatricopeptide repeat-containing protein At1g08070, chloroplastic OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1IT430.0e+0088.08pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita maxima OX=366... [more]
A0A6J1GDX20.0e+0087.44pentatricopeptide repeat-containing protein At5g66520 OS=Cucurbita moschata OX=3... [more]
A0A0A0KKE00.0e+0084.54DYW_deaminase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G5027... [more]
A0A5D3CKZ80.0e+0084.54Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B1S80.0e+0084.54pentatricopeptide repeat-containing protein At5g66520 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G66520.12.0e-20355.38Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G48910.15.6e-15844.84Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G06540.13.9e-15143.77Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G29760.12.0e-14737.99Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G08070.15.8e-14739.92Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 507..527
NoneNo IPR availablePANTHERPTHR47928:SF136PPR CONTAINING PLANT-LIKE PROTEINcoord: 15..595
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 15..595
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 268..364
e-value: 9.9E-25
score: 88.9
coord: 5..129
e-value: 7.1E-14
score: 53.5
coord: 150..267
e-value: 9.8E-27
score: 95.5
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 365..528
e-value: 2.9E-15
score: 58.0
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 181..210
e-value: 5.5E-7
score: 29.4
coord: 387..412
e-value: 3.8E-4
score: 20.5
coord: 152..179
e-value: 1.3
score: 9.4
coord: 212..242
e-value: 1.8E-4
score: 21.6
coord: 286..309
e-value: 0.0026
score: 17.9
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 315..348
e-value: 2.4E-8
score: 31.7
coord: 81..111
e-value: 2.1E-5
score: 22.4
coord: 181..209
e-value: 1.3E-5
score: 23.1
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 312..359
e-value: 2.9E-12
score: 46.6
coord: 77..124
e-value: 1.4E-9
score: 38.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 313..347
score: 12.386327
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 78..112
score: 9.580234
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 179..213
score: 10.939435
IPR032867DYW domainPFAMPF14432DYW_deaminasecoord: 486..611
e-value: 3.8E-38
score: 130.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013415.1Lag0013415.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1900865 chloroplast RNA modification
biological_process GO:0016554 cytidine to uridine editing
cellular_component GO:0009507 chloroplast
molecular_function GO:0003729 mRNA binding
molecular_function GO:0005515 protein binding
molecular_function GO:0008270 zinc ion binding