Lag0013366 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013366
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncytochrome P450 CYP736A12-like
Locationchr1: 49682713 .. 49684206 (+)
RNA-Seq ExpressionLag0013366
SyntenyLag0013366
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACATCAAACTCGGCCTTGTAAACACCATCGTTGTCTCTTCCTCTGAAGCTGCTGAGCTCTTCCTCAAGACCCATGACCTTGTTTTGCGAGTCGCCCACCTAACGAGGTTTTCAAGCACTTGTGTAATGGTCAAAAGGACTTGTTGTTTGCTGAATATGGTCCTTATTGGCGCAGCATGCGCAAAATGTGCACACTCGAGTTGCTTACCAGTCACAAAGTCAATTCTTTCCAATCAATGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGAGAGGCGGCAAAGAATGGAGTTGTTGTCAATCTTAGTTCTAAAATCTCTTCTCTCAATGCAAACATGACCTGTCTGATGATCTTTGGGAAGAAATATGTCGACCAAGAACTTGATGAGAGGGGTTTTAAATGGATGATACAAGAGGCCATGAAATTAGGAGCAATTCCTAACTTGGCTGATTTCATTCCTTATGTTGGAGCACTTGATCTTCAAGGATTGAACCACCGCGCAAAATCTATTGGTAAAGTATTTGACAGATTTTCGAAAGGATTATAGATGAACATCTTCAATCCAAAAGAGATACCAAGGATTTTGTAGATGTCATGTTGGAGATCTTGGGCTCAAAAGAAAATAAGTATCAGGTCAATCGATCTAGCATTAAAGCCATAATTCTTGTAAGTAAACATTTTGCATTCATATTTGATAGGCTTCTCAATTTTGAGGATTTTATAGATTGATCAAGCATGAAAACCAGAATTTAAGGATGTTATTACCTAATGAAAGAATTTATTTAAGAGAGTGAGATGGTTTGCTAGAATTCCTATTTAATACTATATATGCTTTTATAACTATGTTTTGATAAGCATATACTTCATTGGTAGGATATGCTTTTGGCAGCAACTGACACTTCAGCCTCCACCATCGATTGGGCACTATCTGAACTCATTAAACATCCACAGGTTATGAAGAAATTACAAGAAGAAATAAAAAAGTTGTTGGTTTGAATAGGATGGTGGAAGAATCAGACTTGGTACACTTGGAATATTTAGACATGATAGTGAAAGAGATTCTTAGGTTGCATCCTCCAGCTGTGTTATTAGCTCCACATGAATCTCGTGAAGATTGCACAATCAATGACTTCTATATTCCCAAAAGATCACGTGTAATAGTGAACATATGGACAATTGGACGAGACCCAAGTGTTTGGATTGATGCTGAAAAATTCTTTCCTGGAAGATTCATTGGTAGTCAAGTGGACATGAAAGGTAGTTTTCAAATGATTCCATTTGGATATGGTCGAAGAAGTTGTCCTGGAATGCTAATGGGTCTAACCGTGGTTCATCTAGTAATAGCCCAACTTGTGCATTGTTTTGATTTGAAACTTCCAAATGATATGTCACCGGCTCAGTTAGATATGACAGAAGAATTTGGTTTAACAAGTCCAAGAGCTCAAAATCTTATGGCTACTCTTGTTTATCGTCTTCATAACTAA

mRNA sequence

ATGCACATCAAACTCGGCCTTGTAAACACCATCGTTGTCTCTTCCTCTGAAGCTGCTGAGCTCTTCCTCAAGACCCATGACCTTGTTTTGCGAGTCGCCCACCTAACGAGCATGCGCAAAATGTGCACACTCGAGTTGCTTACCAGTCACAAAGTCAATTCTTTCCAATCAATGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGAGAGGCGGCAAAGAATGGAGTTGTTGTCAATCTTAGTTCTAAAATCTCTTCTCTCAATGCAAACATGACCTGTCTGATGATCTTTGGGAAGAAATATGTCGACCAAGAACTTGATGAGAGGGGTTTTAAATGGATGATACAAGAGGCCATGAAATTAGGAGCAATTCCTAACTTGGCTGATTTCATTCCTTATGTTGGAGCACTTGATCTTCAAGGATTGAACCACCGCGCAAAATCTATTGGTTATGAAGAAATTACAAGAAGAAATAAAAAAGTTGTTGGTTTGAATAGGATGGTGGAAGAATCAGACTTGGTACACTTGGAATATTTAGACATGATAGTGAAAGAGATTCTTAGGTTGCATCCTCCAGCTGTGTTATTAGCTCCACATGAATCTCGTGAAGATTGCACAATCAATGACTTCTATATTCCCAAAAGATCACGTGTAATAGTGAACATATGGACAATTGGACGAGACCCAAGTGTTTGGATTGATGCTGAAAAATTCTTTCCTGGAAGATTCATTGGTAGTCAAGTGGACATGAAAGGTAGTTTTCAAATGATTCCATTTGGATATGGTCGAAGAAGTTGTCCTGGAATGCTAATGGGTCTAACCGTGGTTCATCTAGTAATAGCCCAACTTGTGCATTGTTTTGATTTGAAACTTCCAAATGATATGTCACCGGCTCAGTTAGATATGACAGAAGAATTTGGTTTAACAAGTCCAAGAGCTCAAAATCTTATGGCTACTCTTGTTTATCGTCTTCATAACTAA

Coding sequence (CDS)

ATGCACATCAAACTCGGCCTTGTAAACACCATCGTTGTCTCTTCCTCTGAAGCTGCTGAGCTCTTCCTCAAGACCCATGACCTTGTTTTGCGAGTCGCCCACCTAACGAGCATGCGCAAAATGTGCACACTCGAGTTGCTTACCAGTCACAAAGTCAATTCTTTCCAATCAATGAGAAGGGAAGAGCTTGGCTTGTTGATTGACGATCTTCGAGAGGCGGCAAAGAATGGAGTTGTTGTCAATCTTAGTTCTAAAATCTCTTCTCTCAATGCAAACATGACCTGTCTGATGATCTTTGGGAAGAAATATGTCGACCAAGAACTTGATGAGAGGGGTTTTAAATGGATGATACAAGAGGCCATGAAATTAGGAGCAATTCCTAACTTGGCTGATTTCATTCCTTATGTTGGAGCACTTGATCTTCAAGGATTGAACCACCGCGCAAAATCTATTGGTTATGAAGAAATTACAAGAAGAAATAAAAAAGTTGTTGGTTTGAATAGGATGGTGGAAGAATCAGACTTGGTACACTTGGAATATTTAGACATGATAGTGAAAGAGATTCTTAGGTTGCATCCTCCAGCTGTGTTATTAGCTCCACATGAATCTCGTGAAGATTGCACAATCAATGACTTCTATATTCCCAAAAGATCACGTGTAATAGTGAACATATGGACAATTGGACGAGACCCAAGTGTTTGGATTGATGCTGAAAAATTCTTTCCTGGAAGATTCATTGGTAGTCAAGTGGACATGAAAGGTAGTTTTCAAATGATTCCATTTGGATATGGTCGAAGAAGTTGTCCTGGAATGCTAATGGGTCTAACCGTGGTTCATCTAGTAATAGCCCAACTTGTGCATTGTTTTGATTTGAAACTTCCAAATGATATGTCACCGGCTCAGTTAGATATGACAGAAGAATTTGGTTTAACAAGTCCAAGAGCTCAAAATCTTATGGCTACTCTTGTTTATCGTCTTCATAACTAA

Protein sequence

MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRVAHLTSMRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLMIFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIGYEEITRRNKKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKGSFQMIPFGYGRRSCPGMLMGLTVVHLVIAQLVHCFDLKLPNDMSPAQLDMTEEFGLTSPRAQNLMATLVYRLHN
Homology
BLAST of Lag0013366 vs. NCBI nr
Match: XP_022132586.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 438.7 bits (1127), Expect = 4.3e-119
Identity = 244/432 (56.48%), Postives = 282/432 (65.28%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-----------------------VAHLTS 60
           MHIKLGLV+TIVVSS  AAELFLKT+DLV                          ++ ++
Sbjct: 67  MHIKLGLVHTIVVSSPRAAELFLKTYDLVFANRPPSEVFTHIAYGLKNILFAPYGSYWSN 126

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL SHKV SF+SMRREE+GLL +DLREAAK GV V+L+SKI SLN +MTCLM
Sbjct: 127 MRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNLDMTCLM 186

Query: 121 IFGKKYVDQEL-DERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIG---- 180
           IFG+KYV +E+ DERGFKW++QEA++LGA PNL D IP +   DLQGLN RAKS+G    
Sbjct: 187 IFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKSVGKVLD 246

Query: 181 ----------------------------------YEEITRRN------------------ 240
                                               +I R N                  
Sbjct: 247 GFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLATTDTTAT 306

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+ MVEESDL +L+YLDMIVKEILRLHPP  L
Sbjct: 307 TIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRLHPPGPL 366

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKGS-F 329
           L PHES EDCTI++FYIPK+SRVIVN W IGRDPSVWID EKFFP RFIG+QVDMKG  F
Sbjct: 367 LVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVDMKGKHF 426

BLAST of Lag0013366 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 421.0 bits (1081), Expect = 9.4e-114
Identity = 233/432 (53.94%), Postives = 277/432 (64.12%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LG+V TI+V+S +AAELFLKTHDLV                          ++  +
Sbjct: 64  MYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGSYWRN 123

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL+ HKVNSF+SMRREE+GLL+  LR+AAK+ VV+NLSSKI+SLN +MTCLM
Sbjct: 124 MRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLM 183

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI-----G 180
           +FGKKY D E DERGFK +IQEAM+L A PNL DFIP + ALDLQGLN R+K+I     G
Sbjct: 184 VFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFDG 243

Query: 181 Y------EEITRRN---------------------------------------------- 240
           +      E +  +N                                              
Sbjct: 244 FFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSAT 303

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+R VEESDL HLEYLDM VKEILR+HP A L
Sbjct: 304 TVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAPL 363

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SF 329
           L PHES EDCT+N ++IPK+SR+IVN W IG+DP+ W+D EKFFP RFIGSQVD+KG  F
Sbjct: 364 LVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRDF 423

BLAST of Lag0013366 vs. NCBI nr
Match: XP_022950432.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 417.9 bits (1073), Expect = 7.9e-113
Identity = 225/431 (52.20%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-------VAHLT----------------S 60
           MH+KLGLVNTIVVSS EAA LFLKTHDLV           H++                +
Sbjct: 64  MHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGHYWRN 123

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRK+CTLELL++ KVN F+SMR+EEL LLID+LREAAK+ VVVNLSSK+ SL+ANMTCLM
Sbjct: 124 MRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANMTCLM 183

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIGY---- 180
           IFGKK+ D++LDERGFKW++QEA+KL A  NL DFIP++ ALDLQGL  RAK +      
Sbjct: 184 IFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNKVCDR 243

Query: 181 -------EEITRRN---------------------------------------------- 240
                  E +  +N                                              
Sbjct: 244 FFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDSSAVT 303

Query: 241 ----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVLL 300
                                 +KVVGLNRMVEE+DL HL YL+M+VKE+LRLHPP  L+
Sbjct: 304 IGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHPPGPLI 363

Query: 301 APHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SFQ 329
            PHE+ +DCT+N+F+IPK+SR+I+N+W IGR+PS W DAEKFFP RFIGSQVD+KG  FQ
Sbjct: 364 VPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKGKDFQ 423

BLAST of Lag0013366 vs. NCBI nr
Match: XP_023545010.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.9 bits (1073), Expect = 7.9e-113
Identity = 226/431 (52.44%), Postives = 276/431 (64.04%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-------VAHLT----------------S 60
           MH+KLGLVNTIVVSS EAA LFLKTHDLV           H++                +
Sbjct: 64  MHMKLGLVNTIVVSSPEAAALFLKTHDLVYASRPTNEVFKHMSYGERSMVFSKYGHYWRN 123

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRK+CTLELL++ KVN F+SMRREEL LLID+LREAAK+ VVVNLSSK+ SL+ANMTCLM
Sbjct: 124 MRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANMTCLM 183

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIGY---- 180
           IFGKK+ D+ELDERGFKW++QEA+KL A  NL DFIP++ ALDLQGL  RAK +      
Sbjct: 184 IFGKKFEDRELDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNKVCDR 243

Query: 181 -------EEITRRN---------------------------------------------- 240
                  E +  +N                                              
Sbjct: 244 FFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDSSAVT 303

Query: 241 ----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVLL 300
                                 +KVVGLNRMVEE+DL HL YL+M+VKE+LRLHPP  L+
Sbjct: 304 IGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLCHLTYLEMVVKEVLRLHPPGPLI 363

Query: 301 APHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SFQ 329
            PHE+ +DCT+N+F+IPK+SR+I+N+W IGR+PS W DAEKFFP RFIGSQVD+KG  FQ
Sbjct: 364 VPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKGKDFQ 423

BLAST of Lag0013366 vs. NCBI nr
Match: XP_008440675.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa] >TYK12651.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 414.5 bits (1064), Expect = 8.8e-112
Identity = 234/432 (54.17%), Postives = 273/432 (63.19%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LGLV T++VSS +AAELFLKTHD V                           +  +
Sbjct: 63  MYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGPYWRN 122

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL+S KV SF+SMR EELGLL+D LR+AAK  V+VNLSSKI SLN +MTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI------ 180
           +FGKKY DQE DERGFK +IQEAM+L A PNL DFIP +  LDLQGL+ R+K+I      
Sbjct: 183 VFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFDG 242

Query: 181 -----------------------------GYEE----ITRRN------------------ 240
                                        G +E    I R N                  
Sbjct: 243 FLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSAT 302

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+R VEE+DL HLEYLDM+VKEILRLHPPA L
Sbjct: 303 TIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAPL 362

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SF 329
           L PHE+ EDC ++ FYIPK+SR+IVN W IGRDP+ WID EKFFP RFIGSQVD++G  F
Sbjct: 363 LVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRDF 422

BLAST of Lag0013366 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.0e-99
Identity = 200/431 (46.40%), Postives = 261/431 (60.56%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LGL+ TIV+SS EAAELFLKTHDLV                          A+   
Sbjct: 63  MYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGAYWRD 122

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKN-GVVVNLSSKISSLNANMTCL 120
            RKMCT+ELL++HK+NSF+SMRREE+ L ++ +R AA N GV V+LS K+SSL+ +M+C 
Sbjct: 123 TRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDMSCR 182

Query: 121 MIFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI----- 180
           M+ GKKY D+E DERGFK +++EA++L + PNL D+I ++  LDLQG   R KS+     
Sbjct: 183 MVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNKAFD 242

Query: 181 ------------------------------GYEE-------------------------- 240
                                         G EE                          
Sbjct: 243 NLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASMDTSA 302

Query: 241 -------------------ITRRNKKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAV 300
                              + +  + VVGL++MVEESDL  L+YL+M+VKE  RLHP A 
Sbjct: 303 TTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETFRLHPVAP 362

Query: 301 LLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKGS- 327
           LL PH S EDCT+N ++IPK+SRV++N+W IGRDP+ W DAEKF P RF GS VD++G+ 
Sbjct: 363 LLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSSVDVRGNH 422

BLAST of Lag0013366 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.2e-76
Identity = 179/437 (40.96%), Postives = 241/437 (55.15%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLV------LRVA-----------------HLTS 60
           M ++LG V TIVVSS +AAELFLKTHD +      L+ A                 H  +
Sbjct: 68  MSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRN 127

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           +RK   LELLT  K+NSF  MRREELG ++  ++EA+    VV+LS+K++++  NMT  +
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRL 187

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIG----- 180
           + G+     + D    K ++ EA+ L    N+ADF+P++G LD+QGL  + K  G     
Sbjct: 188 LLGR----TKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDK 247

Query: 181 ------------------------------------YEEIT------------------- 240
                                               ++E++                   
Sbjct: 248 ILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAA 307

Query: 241 -------------------RRNKK-------VVGLNRMVEESDLVHLEYLDMIVKEILRL 300
                              R  KK       VVG++RMVEE+DL +LEY+ M+VKE LRL
Sbjct: 308 IDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRL 367

Query: 301 HPPAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWID-AEKFFPGRFIGSQV 327
           HP A LL PHES ED TIN ++IPK+SRVIVN W +GRDP+VW + AE+F P RF GS V
Sbjct: 368 HPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNV 427

BLAST of Lag0013366 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 1.6e-52
Identity = 144/441 (32.65%), Postives = 206/441 (46.71%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRVA-----------------------HLTS 60
           + ++ G V T+VVSSSE A+ FLKTHDL+                           H   
Sbjct: 77  LFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRK 136

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNG-VVVNLSSKISSLNANMTCL 120
           MRK+C LELLTS ++ SF+ +R+EEL  +I  + E +++G + VN+S  IS+  AN+   
Sbjct: 137 MRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWR 196

Query: 121 MIFGKKYVDQEL--DERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHR------- 180
           ++  KK+ D +L  D +GF  ++ E        N+ DFIPY+  LDLQG+          
Sbjct: 197 ILARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANAR 256

Query: 181 -----------------------------------------------------AKSIGYE 240
                                                                 K+I YE
Sbjct: 257 FDAFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYE 316

Query: 241 ----------------------------EITRRNKKVVGLNRMVEESDLVHLEYLDMIVK 300
                                       ++ +  + VVG    V+ESDL  + YL  +VK
Sbjct: 317 LFSAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVK 376

Query: 301 EILRLHPPAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVW-IDAEKFFPGRF 321
           E LRL+P   L  PHES E  T+  +YIPK++ VI+N+W IGRDPSVW  DA +F P RF
Sbjct: 377 ETLRLYPSLPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERF 436

BLAST of Lag0013366 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 133/419 (31.74%), Postives = 196/419 (46.78%), Query Frame = 0

Query: 3   IKLGLVNTIVVSSSEAAELFLKTHDLVLRVAHLTS-----------------------MR 62
           + LG + T++VS++E AE  LKTHDL+      T+                       +R
Sbjct: 68  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 127

Query: 63  KMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLMIF 122
           K+C LELL+  +VNS++S+R EE+GL+++ + ++   G  VNLS  +  L++     + F
Sbjct: 128 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAF 187

Query: 123 GKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALD-LQGLNHRAK--------- 182
           GKKY  +E  +  F  +  E   L     + D+ P    +D L G++ R K         
Sbjct: 188 GKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAF 247

Query: 183 ----------------SIGYEE-------------------ITRRN-------------- 242
                           S G E+                   + R N              
Sbjct: 248 VDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTD 307

Query: 243 ---------------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHP 302
                                      ++VVG    VEE DL  L YL +I+KE LRLHP
Sbjct: 308 TTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHP 367

Query: 303 PAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMK 312
            A LL P ES  D  I  ++IP ++RV +N W IGRDP  W +AE+F P RF+ + VD K
Sbjct: 368 VAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSVDFK 427

BLAST of Lag0013366 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.1e-48
Identity = 136/426 (31.92%), Postives = 196/426 (46.01%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           ++++LG + T+VVSS+  A+  LKTHDL L                         A+   
Sbjct: 83  IYLQLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAYWRH 142

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           +RK+C LELL++ +V SF  +R EE+  L+  + E        NL+  +     ++ C +
Sbjct: 143 IRKICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPG--TTNLTKMLGLYANDVLCRV 202

Query: 121 IFGKKYVD-QELDERGFKWMIQEAMKLGAIPNLADFIPYVGAL-DLQGLNHR-------- 180
            FG+ + +  + D  GF+ M++E  +L    ++ DF P +  +  L G+  R        
Sbjct: 203 AFGRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRF 262

Query: 181 ---------------------------------------------AKSI----------- 240
                                                         K+I           
Sbjct: 263 DELFDQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTDT 322

Query: 241 -------GYEEITRRNK----------KVVGLNRMVEESDLVHLEYLDMIVKEILRLHPP 300
                  G  E+    K           VVG  R+V ESDL  L+Y+  ++KEI RLHPP
Sbjct: 323 TFITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLHPP 382

Query: 301 AVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG 320
           A +L P ES ED TI+ + I  ++R+ VN W IGRDP  W D E F P RFIGS +D KG
Sbjct: 383 APVLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDFKG 442

BLAST of Lag0013366 vs. ExPASy TrEMBL
Match: A0A6J1BSN9 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 2.1e-119
Identity = 244/432 (56.48%), Postives = 282/432 (65.28%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-----------------------VAHLTS 60
           MHIKLGLV+TIVVSS  AAELFLKT+DLV                          ++ ++
Sbjct: 67  MHIKLGLVHTIVVSSPRAAELFLKTYDLVFANRPPSEVFTHIAYGLKNILFAPYGSYWSN 126

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL SHKV SF+SMRREE+GLL +DLREAAK GV V+L+SKI SLN +MTCLM
Sbjct: 127 MRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNLDMTCLM 186

Query: 121 IFGKKYVDQEL-DERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIG---- 180
           IFG+KYV +E+ DERGFKW++QEA++LGA PNL D IP +   DLQGLN RAKS+G    
Sbjct: 187 IFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKSVGKVLD 246

Query: 181 ----------------------------------YEEITRRN------------------ 240
                                               +I R N                  
Sbjct: 247 GFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLATTDTTAT 306

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+ MVEESDL +L+YLDMIVKEILRLHPP  L
Sbjct: 307 TIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRLHPPGPL 366

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKGS-F 329
           L PHES EDCTI++FYIPK+SRVIVN W IGRDPSVWID EKFFP RFIG+QVDMKG  F
Sbjct: 367 LVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVDMKGKHF 426

BLAST of Lag0013366 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 4.5e-114
Identity = 233/432 (53.94%), Postives = 277/432 (64.12%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LG+V TI+V+S +AAELFLKTHDLV                          ++  +
Sbjct: 64  MYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGSYWRN 123

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL+ HKVNSF+SMRREE+GLL+  LR+AAK+ VV+NLSSKI+SLN +MTCLM
Sbjct: 124 MRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDMTCLM 183

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI-----G 180
           +FGKKY D E DERGFK +IQEAM+L A PNL DFIP + ALDLQGLN R+K+I     G
Sbjct: 184 VFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISKIFDG 243

Query: 181 Y------EEITRRN---------------------------------------------- 240
           +      E +  +N                                              
Sbjct: 244 FFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMDTSAT 303

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+R VEESDL HLEYLDM VKEILR+HP A L
Sbjct: 304 TVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHPAAPL 363

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SF 329
           L PHES EDCT+N ++IPK+SR+IVN W IG+DP+ W+D EKFFP RFIGSQVD+KG  F
Sbjct: 364 LVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVKGRDF 423

BLAST of Lag0013366 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 3.8e-113
Identity = 225/431 (52.20%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-------VAHLT----------------S 60
           MH+KLGLVNTIVVSS EAA LFLKTHDLV           H++                +
Sbjct: 64  MHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGHYWRN 123

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRK+CTLELL++ KVN F+SMR+EEL LLID+LREAAK+ VVVNLSSK+ SL+ANMTCLM
Sbjct: 124 MRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANMTCLM 183

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIGY---- 180
           IFGKK+ D++LDERGFKW++QEA+KL A  NL DFIP++ ALDLQGL  RAK +      
Sbjct: 184 IFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNKVCDR 243

Query: 181 -------EEITRRN---------------------------------------------- 240
                  E +  +N                                              
Sbjct: 244 FFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDSSAVT 303

Query: 241 ----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVLL 300
                                 +KVVGLNRMVEE+DL HL YL+M+VKE+LRLHPP  L+
Sbjct: 304 IGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHPPGPLI 363

Query: 301 APHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SFQ 329
            PHE+ +DCT+N+F+IPK+SR+I+N+W IGR+PS W DAEKFFP RFIGSQVD+KG  FQ
Sbjct: 364 VPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKGKDFQ 423

BLAST of Lag0013366 vs. ExPASy TrEMBL
Match: A0A5A7T3U2 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002390 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.2e-112
Identity = 234/432 (54.17%), Postives = 273/432 (63.19%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LGLV T++VSS +AAELFLKTHD V                           +  +
Sbjct: 63  MYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGPYWRN 122

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL+S KV SF+SMR EELGLL+D LR+AAK  V+VNLSSKI SLN +MTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI------ 180
           +FGKKY DQE DERGFK +IQEAM+L A PNL DFIP +  LDLQGL+ R+K+I      
Sbjct: 183 VFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFDG 242

Query: 181 -----------------------------GYEE----ITRRN------------------ 240
                                        G +E    I R N                  
Sbjct: 243 FLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSAT 302

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+R VEE+DL HLEYLDM+VKEILRLHPPA L
Sbjct: 303 TIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAPL 362

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SF 329
           L PHE+ EDC ++ FYIPK+SR+IVN W IGRDP+ WID EKFFP RFIGSQVD++G  F
Sbjct: 363 LVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRDF 422

BLAST of Lag0013366 vs. ExPASy TrEMBL
Match: A0A1S3B1P7 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485015 PE=3 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.2e-112
Identity = 234/432 (54.17%), Postives = 273/432 (63.19%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLRV-----------------------AHLTS 60
           M+++LGLV T++VSS +AAELFLKTHD V                           +  +
Sbjct: 63  MYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFAQYGPYWRN 122

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           MRKMCTLELL+S KV SF+SMR EELGLL+D LR+AAK  V+VNLSSKI SLN +MTCLM
Sbjct: 123 MRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSLNTDMTCLM 182

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSI------ 180
           +FGKKY DQE DERGFK +IQEAM+L A PNL DFIP +  LDLQGL+ R+K+I      
Sbjct: 183 VFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSKAISKIFDG 242

Query: 181 -----------------------------GYEE----ITRRN------------------ 240
                                        G +E    I R N                  
Sbjct: 243 FLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLVAAMDTSAT 302

Query: 241 -----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVL 300
                                  +KVVGL+R VEE+DL HLEYLDM+VKEILRLHPPA L
Sbjct: 303 TIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEILRLHPPAPL 362

Query: 301 LAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG-SF 329
           L PHE+ EDC ++ FYIPK+SR+IVN W IGRDP+ WID EKFFP RFIGSQVD++G  F
Sbjct: 363 LVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQVDVRGRDF 422

BLAST of Lag0013366 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 193.0 bits (489), Expect = 3.9e-49
Identity = 142/448 (31.70%), Postives = 207/448 (46.21%), Query Frame = 0

Query: 2   HIKLGLVNTIVVSSSEAAELFLKTHDLV-------LRVAHLT----------------SM 61
           H+++G ++   VSS E A   L+  D V       + +++LT                 M
Sbjct: 76  HLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQM 135

Query: 62  RKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLMI 121
           RK+C +++ +  +  S+ S+ R+E+  ++  +  +   G  +N+  +I +L  N+T    
Sbjct: 136 RKVCVMKVFSRKRAESWASV-RDEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRAA 195

Query: 122 FGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHR----------- 181
           FG      E  +  F  ++QE  KL    N+ADFIPY G +D QG+N R           
Sbjct: 196 FGSAC---EKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGF 255

Query: 182 ---------------------------------------AKSIGYE-------EITRRNK 241
                                                  AK +          ++TR N 
Sbjct: 256 IDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDNI 315

Query: 242 K-----------------------------------------VVGLNRMVEESDLVHLEY 301
           K                                         VVGL+R VEESD+  L Y
Sbjct: 316 KAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTY 375

Query: 302 LDMIVKEILRLHPPAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKF 327
           L   +KE LR+HPP  LL  HE+ ED +I+ F+IPK+SRV++N + IGRDP+ W D + F
Sbjct: 376 LKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTF 435

BLAST of Lag0013366 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 192.6 bits (488), Expect = 5.0e-49
Identity = 128/437 (29.29%), Postives = 201/437 (46.00%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDL-----------------VLRVA------HLTS 60
           M +KLG V T++VS+ E A+  LK +DL                  L +A      +   
Sbjct: 65  MLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDDYWKE 124

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           +RK+C  EL  + ++NS Q ++  E+  LID + E+A    +VNLS    SLN N+ C  
Sbjct: 125 LRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNVICKA 184

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVG-------------------- 180
           +FG  +    L+   F+ ++ EA+++    + +DF PYVG                    
Sbjct: 185 VFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRERSVRDL 244

Query: 181 ------ALDLQGLNHRAKS-----------------IGYEEITRRNKKVVGLN------- 240
                  +DL    +R +S                 +GY ++TR + K + +N       
Sbjct: 245 DAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNILLGGIN 304

Query: 241 -------------------------------------RMVEESDLVHLEYLDMIVKEILR 300
                                                R++   ++ HL YL+M++KE  R
Sbjct: 305 TSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLNMVIKETCR 364

Query: 301 LHPPAVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQV 327
           LHP A LL P E   +  IN + I  ++R+ VN+W IGRDP +W D E+F P RF+   +
Sbjct: 365 LHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLPERFMDCDI 424

BLAST of Lag0013366 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 190.3 bits (482), Expect = 2.5e-48
Identity = 130/426 (30.52%), Postives = 196/426 (46.01%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDL-----------------VLRVA------HLTS 60
           MH+ LG V T+VVSSS+ A   L+ HDL                  L +A      +   
Sbjct: 65  MHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKE 124

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           +RK+C  EL ++ +V+S Q ++ EE+  +ID + E+A     VNL++K   L  ++ C  
Sbjct: 125 VRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT 184

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGAL-----DLQGLNHRAKS-- 180
            FG  +    L+   F  +++EA+++    + ADFIPYVG +      LQG   R+K   
Sbjct: 185 AFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDL 244

Query: 181 -----------------------------------IGYEEITR----------------- 240
                                              +G +++TR                 
Sbjct: 245 NAFFEQMFDLHKEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGIDT 304

Query: 241 -------------RNKKV-----------VGLNRMVEESDLVHLEYLDMIVKEILRLHPP 300
                        RN +V           +G   M+   D+  LEYL M++KE  RLHP 
Sbjct: 305 SAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKETWRLHPT 364

Query: 301 AVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG 320
             LL P E+  +  IN + IP ++R+ VN+W IGRDP  W D E F P RF+ + +D KG
Sbjct: 365 TPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNIDAKG 424

BLAST of Lag0013366 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 188.7 bits (478), Expect = 7.3e-48
Identity = 128/422 (30.33%), Postives = 197/422 (46.68%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDLVLR-----------------------VAHLTS 60
           M  KLG + T+V+SS E A+  LKTHD VL                        +A    
Sbjct: 68  MSFKLGCLTTVVISSPETAKEVLKTHDHVLSYRVSSDPVRAAGHHELSLLWIPPLARWRF 127

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLS--SKISSLNANMTC 120
           +RK+   +L ++ ++ +  ++R  ++  L++ + +  +    VN+S  S I+SLN     
Sbjct: 128 LRKITRNQLFSTQRLEATSAIRTRKVQELMNFVNKCCERREAVNISRASFITSLNIISNA 187

Query: 121 LMIFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVGALDLQGLNHRAKSIGYE- 180
           L        D       F+ ++   M++   PNLADF P++G LDLQG    A+ + ++ 
Sbjct: 188 LFSTNLANFDDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEARLLMHKL 247

Query: 181 ------------EITRRN------------------------------------------ 240
                         T RN                                          
Sbjct: 248 FRVFQGFIDTKRSSTSRNNNDMLDSLLDIAHKKESELDDNNIKHLLLDLFLAGVDTSSSA 307

Query: 241 ----------------------KKVVGLNRMVEESDLVHLEYLDMIVKEILRLHPPAVLL 300
                                 ++V+GL   V++ D+V L YL  +VKE LRLHPPA  L
Sbjct: 308 VEWAMAELLRNPKMIVKVQEEIRQVIGLKGTVQDLDIVKLPYLQAVVKESLRLHPPAPFL 367

Query: 301 APHESR-EDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKGS-F 319
            P +S  +D  I +F IPK ++V+VN+W IGRDP+VW +  +F P RF+G  +D+KG+ F
Sbjct: 368 VPRKSESDDVQIFEFLIPKNTQVLVNVWAIGRDPNVWKNPTQFEPERFLGRGIDVKGNHF 427

BLAST of Lag0013366 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 127/426 (29.81%), Postives = 188/426 (44.13%), Query Frame = 0

Query: 1   MHIKLGLVNTIVVSSSEAAELFLKTHDL-----------------VLRVA------HLTS 60
           M +K G + T+VVSSSE A+  LK HDL                  L +A      +   
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKE 125

Query: 61  MRKMCTLELLTSHKVNSFQSMRREELGLLIDDLREAAKNGVVVNLSSKISSLNANMTCLM 120
           +R++C  EL +  +V SFQ ++ +E+  LID + E+A  G  VNLS K +SL   +TC  
Sbjct: 126 LRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKA 185

Query: 121 IFGKKYVDQELDERGFKWMIQEAMKLGAIPNLADFIPYVG------------------AL 180
            FG  +    L+   F+ +I +        + +D+ P  G                  AL
Sbjct: 186 TFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVRAL 245

Query: 181 D------------------------LQGLNHRAKSIGYEEITR----------------- 240
           D                        L  L      IGY ++TR                 
Sbjct: 246 DAFYEQMFDLHKQGNKEGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGIGT 305

Query: 241 -------------RNKKV-----------VGLNRMVEESDLVHLEYLDMIVKEILRLHPP 300
                        RN +V           +G   M+   D+  L YL M++ E  RLHPP
Sbjct: 306 SAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWRLHPP 365

Query: 301 AVLLAPHESREDCTINDFYIPKRSRVIVNIWTIGRDPSVWIDAEKFFPGRFIGSQVDMKG 320
           +  L P +   +  +ND+ IP ++R+ VN+W IGRDP  W D E+F P RF+ S +D KG
Sbjct: 366 SPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSIDAKG 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132586.14.3e-11956.48cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_022133318.19.4e-11453.94cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_022950432.17.9e-11352.20cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_023545010.17.9e-11352.44cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_008440675.18.8e-11254.17PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >KAA0036257.1 cytochrom... [more]
Match NameE-valueIdentityDescription
A0A068Q5V63.0e-9946.40Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH181.2e-7640.96Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q50EK41.6e-5232.65Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
P244651.2e-5031.74Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q7211.1e-4831.92Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BSN92.1e-11956.48cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE... [more]
A0A6J1BYU04.5e-11453.94cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
A0A6J1GET03.8e-11352.20cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=... [more]
A0A5A7T3U24.2e-11254.17Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B1P74.2e-11254.17cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485015 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G36220.13.9e-4931.70ferulic acid 5-hydroxylase 1 [more]
AT5G57260.15.0e-4929.29cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
AT3G26310.12.5e-4830.52cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT3G61040.17.3e-4830.33cytochrome P450, family 76, subfamily C, polypeptide 7 [more]
AT3G26320.12.8e-4729.81cytochrome P450, family 71, subfamily B, polypeptide 36 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 157..317
e-value: 1.4E-47
score: 164.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..156
e-value: 4.5E-13
score: 50.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 2..325
IPR001128Cytochrome P450PFAMPF00067p450coord: 161..316
e-value: 2.8E-42
score: 145.0
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 156..327
coord: 1..30
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 36..151
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 36..151
coord: 1..30
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 156..327
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 261..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013366.1Lag0013366.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen