Lag0013359 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013359
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioncytochrome P450 CYP736A12-like
Locationchr1: 49632228 .. 49633853 (+)
RNA-Seq ExpressionLag0013359
SyntenyLag0013359
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTGGATTTGGGCCACAATTTCACTTCTTCTTCTTCATCTTCTTGCTTACCTCTTTCAAACATGGCCATTCAATTCCAAGAACAAGGGCAAGAAATTGCCTCCTGGTCCAAAAGGGTTTCCTATTTTTGGCAGCCTCAATTTGTTAAAACAGTTTCCTCACAGAGATCTTCATCAATTGTCTAAAAAATTTGGTCCCATCATGCACATGCGATTGGGACTTGTAAACGCCATTGTTGTCTCTTCCCCTCAAGCTGCAGAGCTCTTTCTCAAAACCCATGACCTTGTTTTTGCAAGTAGGCCACTTACAATGGTCTCTAAGTTCTTGTCTTATGGGCAAAAGAACTTGGTTTTTGCTCAATATGGATCTTATTGGCGCAATGTTCGAAAAATGTGCACTCTTGAATTGCTCAGCAACCTCAAGATCAACTCTTTCAAATCCATGAGAAAGGAAGAGCTTGGATTGTTGATTGACTACGTTCGAGAGGCTGCAAAGGATGGTGTTGCTATTGATATTAGTTCTAAAGTTTCGTCTCTTATCACAGACATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGCTGATCAAGAACTTGCTGATAGAGGTTTCAAGGCTATGATGCAAGAGGTGATGCAATTAGTGGCTGCTCCTAATTTGGGAGATTATATTCCTTTTATATCCATCTTTGATCTTCAAGGGTTGACTCAGCGGATGAAAAATGTTAGCAAAGTGTTCGATGACTTCTTTGAAATGATTATTGATGAACATCTCAAGTCAAAGGATGAAGAAAGGACTAAGGATTTTTTAGATGTCATGTTCGATCTGATGAATTCTAAAGATACCGAATACCGGGTCGATCGATCCAGTATCAAAGCAATAATATTGGTAAGTTACTTTAATATAATTTGTGGATCAAAAAAGTGATTTTTTTAAAACTATAGAATAAAGGTGTGTGTGTGATATGTGTTTATGAATGTGGGTTTTATTGTATGCTTTTACATTTTATAGGATATACTTGGTGCTGCAATGGACACTTCAGCCACTGTAATTGGATGGGCACTACCTGAGCTCATCAGACATCCACATGTAATGAAGAAAGTGCAGGATGAACTAGAGAATGTGGTAGGCTTGGATAGGATGGTGGAAGAATCAGACTTGATTCACTTGGAATATTTGGACATGGTGATTAAGGAGGTTCTTAGGTTGTATCCGCCTGTTCCATTGTTAGTTCCACATGAGTCTCTTGAAGATTGTATTGTTGATGACTTTTTTATCCCAAAGAAATCACGAATAATAGTGAATGCATGGGCAATTGGACGCAACCCAAGTGCTTGGATTGATCCAGAGAAGTTCTATCCCGAGAGATTCATTAGTAGCCAAGTGGACGTGAGGGGGAGAGATTTTCAATTAATCCCATTTGGAACTGGTCGAAGAGGTTGTCCTGGAATACAATTGGGTTTGATTGTGATTCATTTGGTGCTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGACATGTTAGCCGAAGAATTGGACATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGCCATTCCTATTTATCGACTCCACAATTGA

mRNA sequence

ATGGCTTGGATTTGGGCCACAATTTCACTTCTTCTTCTTCATCTTCTTGCTTACCTCTTTCAAACATGGCCATTCAATTCCAAGAACAAGGGCAAGAAATTGCCTCCTGGTCCAAAAGGGTTTCCTATTTTTGGCAGCCTCAATTTGTTAAAACAGTTTCCTCACAGAGATCTTCATCAATTGTCTAAAAAATTTGGTCCCATCATGCACATGCGATTGGGACTTGTAAACGCCATTGTTGTCTCTTCCCCTCAAGCTGCAGAGCTCTTTCTCAAAACCCATGACCTTGTTTTTGCAAGTAGGCCACTTACAATGGTCTCTAAGTTCTTGTCTTATGGGCAAAAGAACTTGGTTTTTGCTCAATATGGATCTTATTGGCGCAATGTTCGAAAAATGTGCACTCTTGAATTGCTCAGCAACCTCAAGATCAACTCTTTCAAATCCATGAGAAAGGAAGAGCTTGGATTGTTGATTGACTACGTTCGAGAGGCTGCAAAGGATGGTGTTGCTATTGATATTAGTTCTAAAGTTTCGTCTCTTATCACAGACATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGCTGATCAAGAACTTGCTGATAGAGGTTTCAAGGCTATGATGCAAGAGGTGATGCAATTAGTGGCTGCTCCTAATTTGGGAGATTATATTCCTTTTATATCCATCTTTGATCTTCAAGGGTTGACTCAGCGGATGAAAAATGTTAGCAAAGTGTTCGATGACTTCTTTGAAATGATTATTGATGAACATCTCAAGTCAAAGGATGAAGAAAGGACTAAGGATTTTTTAGATGTCATGTTCGATCTGATGAATTCTAAAGATACCGAATACCGGGTCGATCGATCCAGTATCAAAGCAATAATATTGGATATACTTGGTGCTGCAATGGACACTTCAGCCACTGTAATTGGATGGGCACTACCTGAGCTCATCAGACATCCACATGTAATGAAGAAAGTGCAGGATGAACTAGAGAATGTGGTAGGCTTGGATAGGATGGTGGAAGAATCAGACTTGATTCACTTGGAATATTTGGACATGGTGATTAAGGAGGTTCTTAGGTTGTATCCGCCTGTTCCATTGTTAGTTCCACATGAGTCTCTTGAAGATTGTATTGTTGATGACTTTTTTATCCCAAAGAAATCACGAATAATAGTGAATGCATGGGCAATTGGACGCAACCCAAGTGCTTGGATTGATCCAGAGAAGTTCTATCCCGAGAGATTCATTAGTAGCCAAGTGGACGTGAGGGGGAGAGATTTTCAATTAATCCCATTTGGAACTGGTCGAAGAGGTTGTCCTGGAATACAATTGGGTTTGATTGTGATTCATTTGGTGCTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGACATGTTAGCCGAAGAATTGGACATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGCCATTCCTATTTATCGACTCCACAATTGA

Coding sequence (CDS)

ATGGCTTGGATTTGGGCCACAATTTCACTTCTTCTTCTTCATCTTCTTGCTTACCTCTTTCAAACATGGCCATTCAATTCCAAGAACAAGGGCAAGAAATTGCCTCCTGGTCCAAAAGGGTTTCCTATTTTTGGCAGCCTCAATTTGTTAAAACAGTTTCCTCACAGAGATCTTCATCAATTGTCTAAAAAATTTGGTCCCATCATGCACATGCGATTGGGACTTGTAAACGCCATTGTTGTCTCTTCCCCTCAAGCTGCAGAGCTCTTTCTCAAAACCCATGACCTTGTTTTTGCAAGTAGGCCACTTACAATGGTCTCTAAGTTCTTGTCTTATGGGCAAAAGAACTTGGTTTTTGCTCAATATGGATCTTATTGGCGCAATGTTCGAAAAATGTGCACTCTTGAATTGCTCAGCAACCTCAAGATCAACTCTTTCAAATCCATGAGAAAGGAAGAGCTTGGATTGTTGATTGACTACGTTCGAGAGGCTGCAAAGGATGGTGTTGCTATTGATATTAGTTCTAAAGTTTCGTCTCTTATCACAGACATGACTTGTTTGATGGTTTTTGGGAGGAAATTTGCTGATCAAGAACTTGCTGATAGAGGTTTCAAGGCTATGATGCAAGAGGTGATGCAATTAGTGGCTGCTCCTAATTTGGGAGATTATATTCCTTTTATATCCATCTTTGATCTTCAAGGGTTGACTCAGCGGATGAAAAATGTTAGCAAAGTGTTCGATGACTTCTTTGAAATGATTATTGATGAACATCTCAAGTCAAAGGATGAAGAAAGGACTAAGGATTTTTTAGATGTCATGTTCGATCTGATGAATTCTAAAGATACCGAATACCGGGTCGATCGATCCAGTATCAAAGCAATAATATTGGATATACTTGGTGCTGCAATGGACACTTCAGCCACTGTAATTGGATGGGCACTACCTGAGCTCATCAGACATCCACATGTAATGAAGAAAGTGCAGGATGAACTAGAGAATGTGGTAGGCTTGGATAGGATGGTGGAAGAATCAGACTTGATTCACTTGGAATATTTGGACATGGTGATTAAGGAGGTTCTTAGGTTGTATCCGCCTGTTCCATTGTTAGTTCCACATGAGTCTCTTGAAGATTGTATTGTTGATGACTTTTTTATCCCAAAGAAATCACGAATAATAGTGAATGCATGGGCAATTGGACGCAACCCAAGTGCTTGGATTGATCCAGAGAAGTTCTATCCCGAGAGATTCATTAGTAGCCAAGTGGACGTGAGGGGGAGAGATTTTCAATTAATCCCATTTGGAACTGGTCGAAGAGGTTGTCCTGGAATACAATTGGGTTTGATTGTGATTCATTTGGTGCTAGCTCAACTTGTGCATTGTTTTGATTGGAAGCTTCCAAATGACATGTTAGCCGAAGAATTGGACATGACAGAAGAATTTGGTTTAACTTGTCCTAGAGCTCAAGATCTTATAGCCATTCCTATTTATCGACTCCACAATTGA

Protein sequence

MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMKNVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISSQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFGLTCPRAQDLIAIPIYRLHN
Homology
BLAST of Lag0013359 vs. NCBI nr
Match: XP_022950766.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 863.2 bits (2229), Expect = 1.1e-246
Identity = 427/501 (85.23%), Postives = 459/501 (91.62%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M WIWAT++LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLKQFPHRDLH+
Sbjct: 1   MPWIWATLALLLLS--HYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K GPIMHM+LGLVN IVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++AA DGVA+D+SSKV SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD+IPFI+ FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKD--EERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDI 300
           NVS+VFD FFE IIDEHL SK   E+RTKDFLDVM DL+ S+DTEYRVDRSS+KAIILDI
Sbjct: 241 NVSQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDTEYRVDRSSVKAIILDI 300

Query: 301 LGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKE 360
           LGAAMDTSATVIGWALPELIRHP VMKKVQDELENVVGLDRMVEESDLIHLEYLDMV KE
Sbjct: 301 LGAAMDTSATVIGWALPELIRHPDVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVTKE 360

Query: 361 VLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFIS 420
           +LRLYPPVPLLVPHESL+DCIVDDFFIPKKSR+IVNAWAIGRNPSAW +PEKFYPERF  
Sbjct: 361 ILRLYPPVPLLVPHESLQDCIVDDFFIPKKSRVIVNAWAIGRNPSAWANPEKFYPERFTG 420

Query: 421 SQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTE 480
           SQVDVRGRDFQLIPFG GRRGCPGIQLGLIV  LV+AQLVHCF WKL NDM  +ELDMTE
Sbjct: 421 SQVDVRGRDFQLIPFGAGRRGCPGIQLGLIVTRLVVAQLVHCFHWKLSNDMSVDELDMTE 480

Query: 481 EFGLTCPRAQDLIAIPIYRLH 500
           EFGLTCPRA +L  IPIYRLH
Sbjct: 481 EFGLTCPRALELTVIPIYRLH 499

BLAST of Lag0013359 vs. NCBI nr
Match: XP_023543389.1 (cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 859.4 bits (2219), Expect = 1.6e-245
Identity = 423/501 (84.43%), Postives = 460/501 (91.82%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M WIWAT++LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLKQFPHRDLH+
Sbjct: 1   MPWIWATLALLLLS--HYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K GPIMHM+LGLVN IVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++AA DGVA+D+SSKV SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD+IPFIS FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFISTFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKD--EERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDI 300
           NVS+VFD FFE IIDEHLKSK   E+RTKDFLDVM DL+ S+DTEYR+DRSS+KAIILDI
Sbjct: 241 NVSQVFDGFFERIIDEHLKSKSFGEKRTKDFLDVMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKE 360
           LGAAMDTSATVIGWALPELIRHP VMK+VQDELE+VVGLDRMVEESDLIHLEYLDMVIKE
Sbjct: 301 LGAAMDTSATVIGWALPELIRHPDVMKRVQDELEDVVGLDRMVEESDLIHLEYLDMVIKE 360

Query: 361 VLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFIS 420
           +LRLYPPVPLLVPHESL+DCIV DFFIPKKSR+IVNAWAIGRNP+ W +P+KFYPERF  
Sbjct: 361 ILRLYPPVPLLVPHESLQDCIVGDFFIPKKSRVIVNAWAIGRNPNVWANPKKFYPERFNG 420

Query: 421 SQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTE 480
           SQ+DVRGRDFQLIPFG GRRGCPGIQLGL V  LV+AQLVHCF WKL NDM  +EL+MTE
Sbjct: 421 SQIDVRGRDFQLIPFGAGRRGCPGIQLGLTVTRLVVAQLVHCFHWKLSNDMSVDELNMTE 480

Query: 481 EFGLTCPRAQDLIAIPIYRLH 500
           EFGLTCPRAQDLI IPIYRLH
Sbjct: 481 EFGLTCPRAQDLIVIPIYRLH 499

BLAST of Lag0013359 vs. NCBI nr
Match: XP_038883676.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 857.4 bits (2214), Expect = 6.0e-245
Identity = 422/500 (84.40%), Postives = 455/500 (91.00%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWIWATI+LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLK+FPHRDLHQ
Sbjct: 1   MAWIWATIALLLLS--NYLFQTWQFNKKSKGKKLPPGPKGFPIIGSLNLLKEFPHRDLHQ 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+GPIMHM+LGLVN IVVSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKYGPIMHMKLGLVNTIVVSSPTAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRN+RKMCTLELLSN KINSFKSMRKEELGLLI++++EAA DGVAI++SSKV SL
Sbjct: 121 QYGSYWRNIRKMCTLELLSNHKINSFKSMRKEELGLLIEHLKEAANDGVAINLSSKVVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAMMQEVMQL+AAPNLGD+IPFI+  DLQGLT RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMMQEVMQLIAAPNLGDFIPFIARLDLQGLTCRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDT-EYRVDRSSIKAIILDIL 300
           NVSKVFD FFE IIDEHL S   +RTKDFLDVM DLM+S DT EYR+DRS +KAII+DIL
Sbjct: 241 NVSKVFDGFFERIIDEHLNSMGGKRTKDFLDVMLDLMDSVDTHEYRIDRSCVKAIIMDIL 300

Query: 301 GAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEV 360
           GAAMDTSATVIGWALPELIRHP  MKK+QDELENVVGLDR+V+ESDLIHLEYLDMVIKEV
Sbjct: 301 GAAMDTSATVIGWALPELIRHPQAMKKMQDELENVVGLDRIVQESDLIHLEYLDMVIKEV 360

Query: 361 LRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISS 420
           LRLYPPVPLLVPHESLEDCIV+DFFIPKKSRIIVN W IGRNPS WI PEKFYPERFI  
Sbjct: 361 LRLYPPVPLLVPHESLEDCIVNDFFIPKKSRIIVNTWTIGRNPSTWIHPEKFYPERFIGG 420

Query: 421 QVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEE 480
           +VDVRGRDFQLIPFGTGRRGCPG+QLGL V+ LV+AQLVHCF WKLP  M  ++LDMTEE
Sbjct: 421 KVDVRGRDFQLIPFGTGRRGCPGLQLGLTVVRLVIAQLVHCFHWKLPKRMTIDKLDMTEE 480

Query: 481 FGLTCPRAQDLIAIPIYRLH 500
           FGLTCPRAQDLI  PIYRLH
Sbjct: 481 FGLTCPRAQDLIVTPIYRLH 498

BLAST of Lag0013359 vs. NCBI nr
Match: XP_022978476.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 417/501 (83.23%), Postives = 460/501 (91.82%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWIWAT++LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLKQFPHRDLH+
Sbjct: 1   MAWIWATLALLLLS--HYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+GPIMHM+LGLVN  VVSS +AAELFLKTHDLVFASRPLTMVSKFLSYGQKNL+FA
Sbjct: 61  LSQKYGPIMHMKLGLVNTTVVSSAEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLIFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+Y+++AA DGVA+D+SSK+ SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIEYLKKAANDGVAVDLSSKIVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD+IPFI+ FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKD--EERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDI 300
            VS+VFD FFE IIDEHLKS+   E+RTKDFLD M DL+ S+DTEYR+DRSS+KAIILDI
Sbjct: 241 IVSQVFDGFFERIIDEHLKSRSLGEKRTKDFLDFMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKE 360
           LGAAMDTSATVIGWALPELIRHP VM KVQDELE+VVGLDRMVEESDLIHLEYLDMVIKE
Sbjct: 301 LGAAMDTSATVIGWALPELIRHPDVMMKVQDELEHVVGLDRMVEESDLIHLEYLDMVIKE 360

Query: 361 VLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFIS 420
           +LRLYPPVPLLVPHESL+DCI+DD+FIPKKSR+I+NAWAIGRNPS+W +PEKFYPERF  
Sbjct: 361 ILRLYPPVPLLVPHESLQDCIIDDYFIPKKSRVILNAWAIGRNPSSWANPEKFYPERFNG 420

Query: 421 SQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTE 480
           SQVDVRGR FQLIPFG GRRGCPG+QLGL V HLV+AQLVHCF WKL NDM  +ELDMTE
Sbjct: 421 SQVDVRGRHFQLIPFGAGRRGCPGMQLGLTVTHLVVAQLVHCFHWKLSNDMSVDELDMTE 480

Query: 481 EFGLTCPRAQDLIAIPIYRLH 500
           EFGLTCPRAQDLI +PIYRLH
Sbjct: 481 EFGLTCPRAQDLIVVPIYRLH 499

BLAST of Lag0013359 vs. NCBI nr
Match: XP_004143494.1 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 854.0 bits (2205), Expect = 6.6e-244
Identity = 416/500 (83.20%), Postives = 458/500 (91.60%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWIWATI  LL  L  Y+F+TW F  K+KGKKLPPGPKGFPIFGSLNLLK+FPHRDLH+
Sbjct: 1   MAWIWATIIALLFLLSNYIFETWLFKKKSKGKKLPPGPKGFPIFGSLNLLKEFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+GPIMH++LGLVN I+VSSP AAELFLKTHDL+FASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKYGPIMHIKLGLVNTIIVSSPPAAELFLKTHDLIFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLSN KINSFKSMRKEE+GLLI+Y++EAA DGV+++ISSKV SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSNHKINSFKSMRKEEVGLLIEYLKEAANDGVSVNISSKVVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD IPFI++FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDLIPFIAMFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDT-EYRVDRSSIKAIILDIL 300
           N++KVFD FFE IIDEHLKS  E++TKDFLDVM DLM S+DT EYR+DRSS+KAIILD+L
Sbjct: 241 NINKVFDRFFERIIDEHLKSMGEKKTKDFLDVMLDLMKSEDTHEYRIDRSSVKAIILDVL 300

Query: 301 GAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEV 360
           GAAMDTSATVIGWAL ELIRHP VMKKVQDE+ENVVGLDRMVEE DLIHLEYLDMVIKE+
Sbjct: 301 GAAMDTSATVIGWALAELIRHPDVMKKVQDEVENVVGLDRMVEELDLIHLEYLDMVIKEI 360

Query: 361 LRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISS 420
           LRLYPP+PLLVPHESL+DCIV+DFFIPK SR+IVNAWAIGRNPSAWI P KF PERF+ S
Sbjct: 361 LRLYPPLPLLVPHESLQDCIVEDFFIPKNSRVIVNAWAIGRNPSAWIHPLKFCPERFMGS 420

Query: 421 QVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEE 480
            +DVRGRDFQLIPFG GRRGCPGIQLG+ VI LVLAQL+HCFDWKLPN    +ELDMTEE
Sbjct: 421 HLDVRGRDFQLIPFGAGRRGCPGIQLGMTVIRLVLAQLLHCFDWKLPNGTSIDELDMTEE 480

Query: 481 FGLTCPRAQDLIAIPIYRLH 500
           FGLTCPRAQDLI IPIYRLH
Sbjct: 481 FGLTCPRAQDLIVIPIYRLH 500

BLAST of Lag0013359 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 4.2e-193
Identity = 328/499 (65.73%), Postives = 408/499 (81.76%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M WIWATI LL    L ++ Q W    KNK K+LPPGP+GFPIFGSL+LL +FP++DLH+
Sbjct: 1   MVWIWATIGLL---ALVHILQAW---WKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           L++K+G IM+MRLGL+  IV+SSP+AAELFLKTHDLVFASRP    SK +S+GQKNL+F+
Sbjct: 61  LARKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFS 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKD-GVAIDISSKVSS 180
           +YG+YWR+ RKMCT+ELLSN KINSFKSMR+EE+ L ++ +R AA + GVA+D+S KVSS
Sbjct: 121 EYGAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSS 180

Query: 181 LITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRM 240
           L  DM+C MV G+K+ D+E  +RGFK++++E +QL +APNLGDYI FI+  DLQG T+RM
Sbjct: 181 LSVDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRM 240

Query: 241 KNVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDIL 300
           K+V+K FD+ FE II+EHL+  D ERT DF+DVM   M S+++EYR++R  IKAI+LD+L
Sbjct: 241 KSVNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDML 300

Query: 301 GAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEV 360
            A+MDTSAT I WAL EL+RHP  MKKVQ ELENVVGLD+MVEESDL  L+YL+MV+KE 
Sbjct: 301 VASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKET 360

Query: 361 LRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISS 420
            RL+P  PLL+PH S+EDC V+ + IPKKSR+++N WAIGR+P+AW D EKF PERF  S
Sbjct: 361 FRLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGS 420

Query: 421 QVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEE 480
            VDVRG  FQLIPFG+GRR CPGIQLGL V+ LVLAQLVHCFDW+LPN+ML EELDMTEE
Sbjct: 421 SVDVRGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEE 480

Query: 481 FGLTCPRAQDLIAIPIYRL 499
           FGLT PRA+ L+AIP YRL
Sbjct: 481 FGLTVPRAKHLLAIPSYRL 493

BLAST of Lag0013359 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.1e-143
Identity = 254/473 (53.70%), Postives = 335/473 (70.82%), Query Frame = 0

Query: 33  KLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGPIMHMRLGLVNAIVVSSPQAAELFLK 92
           KLPPGP+G PI GSL+ L   PHR L  L+KK+GPIM MRLG V  IVVSSPQAAELFLK
Sbjct: 32  KLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAELFLK 91

Query: 93  THDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKMCTLELLSNLKINSFKSMRKE 152
           THD +FASRP    ++++SYG   + F  YG +WRN+RK   LELL+  KINSF  MR+E
Sbjct: 92  THDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGMRRE 151

Query: 153 ELGLLIDYVREAAKDGVAIDISSKVSSLITDMTCLMVFGRKFADQELADRGFKAMMQEVM 212
           ELG ++  ++EA+     +D+S+KV+++I +MT  ++ GR   D+       K +M E +
Sbjct: 152 ELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRTKDDR----YDLKGIMNEAL 211

Query: 213 QLVAAPNLGDYIPFISIFDLQGLTQRMKNVSKVFDDFFEMIIDEH-LKSKDEERTKDFLD 272
            L    N+ D++PF+   D+QGLT++ K+  K  D   E IIDEH   S +   + DF+D
Sbjct: 212 TLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGDFID 271

Query: 273 VMFDLMNSKDTEYR-----VDRSSIKAIILDILGAAMDTSATVIGWALPELIRHPHVMKK 332
            M  L N     +      +DRS IKAI++DI+ AA+DTS T I W L ELI+HP  MKK
Sbjct: 272 DMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAMKK 331

Query: 333 VQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLRLYPPVPLLVPHESLEDCIVDDFFIP 392
            Q+E++ VVG+DRMVEE+DL +LEY+ MV+KE LRL+P  PLL PHES+ED  ++ +FIP
Sbjct: 332 CQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYFIP 391

Query: 393 KKSRIIVNAWAIGRNPSAWID-PEKFYPERFISSQVDVRGRDFQLIPFGTGRRGCPGIQL 452
           K+SR+IVN+WA+GR+P+ W +  E+F PERF  S VDVRGRDFQL+PFG+GRRGCPG+QL
Sbjct: 392 KQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNVDVRGRDFQLLPFGSGRRGCPGMQL 451

Query: 453 GLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFGLTCPRAQDLIAIPIYRL 499
           GLI + LV+A+LVHCFDW LPN    + LDMTE+FGLT PR + L+A+P YRL
Sbjct: 452 GLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFGLTTPRVKHLLAVPKYRL 500

BLAST of Lag0013359 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.0e-114
Identity = 209/505 (41.39%), Postives = 318/505 (62.97%), Query Frame = 0

Query: 11  LLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGPIMH 70
           +L+    + F  W  +   + ++LPPGP  +PI G+ + ++   HR L  L++K+GPI+ 
Sbjct: 19  ILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILF 78

Query: 71  MRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVR 130
           +R G V  +VVSS + A+ FLKTHDL+FASRP T V K+  Y  K++ F+ YG +WR +R
Sbjct: 79  LRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMR 138

Query: 131 KMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDG-VAIDISSKVSSLITDMTCLMV 190
           K+C LELL++ +I SFK +R+EEL  +I  + E ++ G +A+++S  +S+ + ++   ++
Sbjct: 139 KICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRIL 198

Query: 191 FGRKFADQELA--DRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMKNVSKVFD 250
             +KF+D +L    +GF  ++ EV   V + N+GD+IP++   DLQG+ + +K  +  FD
Sbjct: 199 ARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFD 258

Query: 251 DFFEMIIDEHLKSKDEE--------RTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDIL 310
            F E +IDEH+ +              KD +DV+ ++  + +T  +V R  IKAI  ++ 
Sbjct: 259 AFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELF 318

Query: 311 GAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEV 370
            A M+TSA V+ WA+ EL+RHPH MKK+Q E+E+VVG    V+ESDL  + YL  V+KE 
Sbjct: 319 SAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKET 378

Query: 371 LRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAW-IDPEKFYPERFIS 430
           LRLYP +PL +PHESLE   V  ++IPKK+ +I+N WAIGR+PS W  D  +F PERF+ 
Sbjct: 379 LRLYPSLPLALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQ 438

Query: 431 SQ---VDVRG--RDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEE 490
            +   +D+ G   DF+++PFG GRR CPG  + ++ +   LAQL+H FDW++  D    E
Sbjct: 439 MEENGIDLSGGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEGD--PSE 498

Query: 491 LDMTEEFGLTCPRAQDLIAIPIYRL 499
           LDM E      PR   L+A P  RL
Sbjct: 499 LDMKEACATKMPRQTPLLAYPRLRL 521

BLAST of Lag0013359 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.6e-113
Identity = 206/486 (42.39%), Postives = 311/486 (63.99%), Query Frame = 0

Query: 11  LLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGPIMH 70
           LL+ LL ++++     S+ +  KLPP P   P+ G+L+ L   PH  L  L++K+GPI++
Sbjct: 28  LLILLLKFIYKD---KSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKYGPIIY 87

Query: 71  MRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVR 130
           ++LG +  +VVSS + A+  LKTHDL  +SRP    +K L Y   ++VF+ YG+YWR++R
Sbjct: 88  LQLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAYWRHIR 147

Query: 131 KMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDMTCLMVF 190
           K+C LELLS  ++ SF  +R+EE+  L+  V E        +++  +     D+ C + F
Sbjct: 148 KICILELLSAKRVQSFSHVREEEVARLVRRVAEFYPG--TTNLTKMLGLYANDVLCRVAF 207

Query: 191 GRKFADQELADR-GFKAMMQEVMQLVAAPNLGDYIPFIS-IFDLQGLTQRMKNVSKVFDD 250
           GR F++    DR GF+ M++E  +L+   ++GD+ P +  I  L G+  R++   + FD+
Sbjct: 208 GRGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRFDE 267

Query: 251 FFEMIIDEHLKSK-DEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILGAAMDTSA 310
            F+ ++ +HL  K ++E  KD +DV+ D+   + TE  +   ++KAIILD+  A  DT+ 
Sbjct: 268 LFDQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTDTTF 327

Query: 311 TVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLRLYPPVP 370
             + W + EL+ +  V+++ Q E+  VVG  R+V ESDL  L+Y+  VIKE+ RL+PP P
Sbjct: 328 ITLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLHPPAP 387

Query: 371 LLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISSQVDVRGRD 430
           +LVP ES+ED  +D + I  K+RI VNAWAIGR+P +W DPE F PERFI S +D +G+D
Sbjct: 388 VLVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDFKGQD 447

Query: 431 FQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFGLTCPRA 490
           F+LIPFG GRRGCP +  G   I L LAQL+H FDW+LP D  A++LDMTE FG+T  R 
Sbjct: 448 FELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGITMHRI 507

Query: 491 QDLIAI 494
            +LI +
Sbjct: 508 ANLIVV 508

BLAST of Lag0013359 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-111
Identity = 199/482 (41.29%), Postives = 299/482 (62.03%), Query Frame = 0

Query: 8   ISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGP 67
           +SLL L +    F       + K   LPP P   PI G+L+ L   PHR L  L+ + GP
Sbjct: 5   VSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGP 64

Query: 68  IMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWR 127
           ++ + LG +  ++VS+ + AE  LKTHDL+FASRP T  ++ + Y   ++ F+ YG YWR
Sbjct: 65  LILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWR 124

Query: 128 NVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDMTCL 187
            VRK+C LELLS  ++NS++S+R+EE+GL+++ + ++   G A+++S  +  L +     
Sbjct: 125 QVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITR 184

Query: 188 MVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFD-LQGLTQRMKNVSKVF 247
           + FG+K+  +E     F  +  E+  L+ A  +GDY P  +  D L G+  R+K      
Sbjct: 185 VAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGEL 244

Query: 248 DDFFEMIIDEHLKSK-----DEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILGA 307
           D F + +ID+HL S+     D    KD +DV+  L         ++R+++KA+ILD+   
Sbjct: 245 DAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSG 304

Query: 308 AMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLR 367
             DT+A  + WA+ ELI+HP VM+K Q E+  VVG    VEE DL  L YL ++IKE LR
Sbjct: 305 GTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLR 364

Query: 368 LYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISSQV 427
           L+P  PLLVP ES  D ++  + IP K+R+ +NAWAIGR+P +W + E+F PERF+++ V
Sbjct: 365 LHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSV 424

Query: 428 DVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFG 484
           D +G+DFQLIPFG GRRGCPGI  G+  + + LA L++ F+W+LP D+  E+LDM+E  G
Sbjct: 425 DFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVG 484

BLAST of Lag0013359 vs. ExPASy TrEMBL
Match: A0A6J1GFR1 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.3e-247
Identity = 427/501 (85.23%), Postives = 459/501 (91.62%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M WIWAT++LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLKQFPHRDLH+
Sbjct: 1   MPWIWATLALLLLS--HYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K GPIMHM+LGLVN IVVSSP+AAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKHGPIMHMKLGLVNTIVVSSPEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+ +++AA DGVA+D+SSKV SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIECLKKAANDGVAVDLSSKVVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD+IPFI+ FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKD--EERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDI 300
           NVS+VFD FFE IIDEHL SK   E+RTKDFLDVM DL+ S+DTEYRVDRSS+KAIILDI
Sbjct: 241 NVSQVFDGFFERIIDEHLMSKSLGEKRTKDFLDVMLDLIGSEDTEYRVDRSSVKAIILDI 300

Query: 301 LGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKE 360
           LGAAMDTSATVIGWALPELIRHP VMKKVQDELENVVGLDRMVEESDLIHLEYLDMV KE
Sbjct: 301 LGAAMDTSATVIGWALPELIRHPDVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVTKE 360

Query: 361 VLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFIS 420
           +LRLYPPVPLLVPHESL+DCIVDDFFIPKKSR+IVNAWAIGRNPSAW +PEKFYPERF  
Sbjct: 361 ILRLYPPVPLLVPHESLQDCIVDDFFIPKKSRVIVNAWAIGRNPSAWANPEKFYPERFTG 420

Query: 421 SQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTE 480
           SQVDVRGRDFQLIPFG GRRGCPGIQLGLIV  LV+AQLVHCF WKL NDM  +ELDMTE
Sbjct: 421 SQVDVRGRDFQLIPFGAGRRGCPGIQLGLIVTRLVVAQLVHCFHWKLSNDMSVDELDMTE 480

Query: 481 EFGLTCPRAQDLIAIPIYRLH 500
           EFGLTCPRA +L  IPIYRLH
Sbjct: 481 EFGLTCPRALELTVIPIYRLH 499

BLAST of Lag0013359 vs. ExPASy TrEMBL
Match: A0A6J1IMS2 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.4e-245
Identity = 417/501 (83.23%), Postives = 460/501 (91.82%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWIWAT++LLLL    YLFQTW FN K+KGKKLPPGPKGFPI GSLNLLKQFPHRDLH+
Sbjct: 1   MAWIWATLALLLLS--HYLFQTWRFNIKSKGKKLPPGPKGFPIIGSLNLLKQFPHRDLHR 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+GPIMHM+LGLVN  VVSS +AAELFLKTHDLVFASRPLTMVSKFLSYGQKNL+FA
Sbjct: 61  LSQKYGPIMHMKLGLVNTTVVSSAEAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLIFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLS  KINSFKSMRKEE+GL+I+Y+++AA DGVA+D+SSK+ SL
Sbjct: 121 QYGSYWRNVRKMCTLELLSTQKINSFKSMRKEEIGLVIEYLKKAANDGVAVDLSSKIVSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFGRKF D+EL DRGFKAM+QEVMQLVAAPNLGD+IPFI+ FDLQGLT+RMK
Sbjct: 181 STDMTCLMVFGRKFGDEELDDRGFKAMIQEVMQLVAAPNLGDFIPFIATFDLQGLTRRMK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKD--EERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDI 300
            VS+VFD FFE IIDEHLKS+   E+RTKDFLD M DL+ S+DTEYR+DRSS+KAIILDI
Sbjct: 241 IVSQVFDGFFERIIDEHLKSRSLGEKRTKDFLDFMLDLIGSEDTEYRIDRSSVKAIILDI 300

Query: 301 LGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKE 360
           LGAAMDTSATVIGWALPELIRHP VM KVQDELE+VVGLDRMVEESDLIHLEYLDMVIKE
Sbjct: 301 LGAAMDTSATVIGWALPELIRHPDVMMKVQDELEHVVGLDRMVEESDLIHLEYLDMVIKE 360

Query: 361 VLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFIS 420
           +LRLYPPVPLLVPHESL+DCI+DD+FIPKKSR+I+NAWAIGRNPS+W +PEKFYPERF  
Sbjct: 361 ILRLYPPVPLLVPHESLQDCIIDDYFIPKKSRVILNAWAIGRNPSSWANPEKFYPERFNG 420

Query: 421 SQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTE 480
           SQVDVRGR FQLIPFG GRRGCPG+QLGL V HLV+AQLVHCF WKL NDM  +ELDMTE
Sbjct: 421 SQVDVRGRHFQLIPFGAGRRGCPGMQLGLTVTHLVVAQLVHCFHWKLSNDMSVDELDMTE 480

Query: 481 EFGLTCPRAQDLIAIPIYRLH 500
           EFGLTCPRAQDLI +PIYRLH
Sbjct: 481 EFGLTCPRAQDLIVVPIYRLH 499

BLAST of Lag0013359 vs. ExPASy TrEMBL
Match: A0A6J1BWX1 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.6e-241
Identity = 416/501 (83.03%), Postives = 454/501 (90.62%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M WIWATI+LLLL L  YL Q W FNS    KKLPPGPKGFPI G LNLL +FPHRDL +
Sbjct: 1   MPWIWATIALLLL-LANYLLQPWLFNS----KKLPPGPKGFPILGCLNLLGKFPHRDLQK 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+GPIMHMRLGLVN IVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA
Sbjct: 61  LSQKYGPIMHMRLGLVNTIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLID VRE A DG  +D+SS+VSSL
Sbjct: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDRVREKANDGAVVDLSSEVSSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDY-IPFISIFDLQGLTQRM 240
            TDMTCLMVFGRKF+D+E  DRGFK M+QEVMQLVAAPNLGD+ IPF++ FDLQGLT+RM
Sbjct: 181 STDMTCLMVFGRKFSDEEFDDRGFKTMIQEVMQLVAAPNLGDFNIPFVATFDLQGLTRRM 240

Query: 241 KNVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDIL 300
           KNVSKVFD+FFE IIDEHLK    +R+KDF+DVM DLM S++TEY++DRSSIKAI+LDIL
Sbjct: 241 KNVSKVFDEFFERIIDEHLKPMGAKRSKDFVDVMLDLMGSEETEYQIDRSSIKAIMLDIL 300

Query: 301 GAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEV 360
           GAAMDTSATVIGWALPELI++P VMKKVQDELE VVGLDRMVEESDL++LEYLDMVIKE 
Sbjct: 301 GAAMDTSATVIGWALPELIKNPQVMKKVQDELEKVVGLDRMVEESDLVNLEYLDMVIKET 360

Query: 361 LRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISS 420
           LRLYPPVPLLVPHESLEDC+V+DF IPKKSR+IVNAWAIGRNPS W DPEKFYPERF+ S
Sbjct: 361 LRLYPPVPLLVPHESLEDCVVNDFHIPKKSRVIVNAWAIGRNPSTWTDPEKFYPERFVDS 420

Query: 421 QVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEE 480
           QVDVRGRDFQLIPFG GRRGCPGIQLGL V+ L+LAQLVHCFDWKLPNDML+ +LDMTEE
Sbjct: 421 QVDVRGRDFQLIPFGAGRRGCPGIQLGLTVVRLILAQLVHCFDWKLPNDMLSVQLDMTEE 480

Query: 481 FGLTCPRAQDLIAIPIYRLHN 501
           FGLTCPRAQDLI  PIYR+H+
Sbjct: 481 FGLTCPRAQDLIVTPIYRIHS 496

BLAST of Lag0013359 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 3.9e-210
Identity = 353/500 (70.60%), Postives = 430/500 (86.00%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWI   ++L  L LL  + + W F S+N   KLPPGP+GFP+FGSL+LL + PHRDL  
Sbjct: 1   MAWILLAVALAGLALL--ILKPWLFRSQN---KLPPGPRGFPVFGSLHLLGKLPHRDLRD 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LS+K+G IM+MRLG+V  I+V+SPQAAELFLKTHDLVFASRP    SK++SYGQKNL FA
Sbjct: 61  LSQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYGSYWRN+RKMCTLELLS+ K+NSF+SMR+EE+GLL+ Y+R+AAKD V I++SSK++SL
Sbjct: 121 QYGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFG+K+AD E  +RGFK+++QE MQLVA PNLGD+IP I+  DLQGL +R K
Sbjct: 181 NTDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILG 300
            +SK+FD FFE IIDEHL+SK+E +TKDF+DVM D+M S++TEY++DRSSIKAIILD+L 
Sbjct: 241 AISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLV 300

Query: 301 AAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVL 360
           AAMDTSAT +GWA+ ELI+HP VMKK+Q+EL+ VVGLDR VEESDL HLEYLDM +KE+L
Sbjct: 301 AAMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEIL 360

Query: 361 RLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISSQ 420
           R++P  PLLVPHESLEDC V+ + IPKKSRIIVNAWAIG++P++W+DPEKF+PERFI SQ
Sbjct: 361 RVHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQ 420

Query: 421 VDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEF 480
           VDV+GRDFQLIPFG+GRRGCPG+ +GL+V+H V+AQLVHCFDWKLPN ML  ELDMTEEF
Sbjct: 421 VDVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEF 480

Query: 481 GLTCPRAQDLIAIPIYRLHN 501
           GLTCPRAQDL+  PIYRL N
Sbjct: 481 GLTCPRAQDLMVTPIYRLQN 495

BLAST of Lag0013359 vs. ExPASy TrEMBL
Match: A0A5A7T3U2 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002390 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 8.8e-210
Identity = 355/500 (71.00%), Postives = 428/500 (85.60%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           MAWI+  ++L+    LA L +T  + S N   KLPPGP+GFPIFG L+LL + PHRDL  
Sbjct: 1   MAWIFLAVALV---GLAILLRTRLYRSPN---KLPPGPRGFPIFGCLHLLGKLPHRDLRS 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LSKK+G IM+MRLGLV  ++VSSPQAAELFLKTHD VFASRP    SK++SYGQKNL FA
Sbjct: 61  LSKKYGSIMYMRLGLVPTVIVSSPQAAELFLKTHDTVFASRPFVQASKYMSYGQKNLGFA 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           QYG YWRN+RKMCTLELLS++K+ SF+SMR EELGLL+DY+R+AAK  V +++SSK+ SL
Sbjct: 121 QYGPYWRNMRKMCTLELLSSVKVKSFRSMRMEELGLLVDYLRDAAKKRVIVNLSSKICSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMK 240
            TDMTCLMVFG+K+ DQE  +RGFK+++QE MQLVA+PNLGD+IP I+  DLQGL +R K
Sbjct: 181 NTDMTCLMVFGKKYEDQEFDERGFKSVIQEAMQLVASPNLGDFIPQIAGLDLQGLDRRSK 240

Query: 241 NVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILG 300
            +SK+FD F E IIDEHL+SK+E +T DF+DVM ++M S++TEY+++RS+IKA+ILD+L 
Sbjct: 241 AISKIFDGFLERIIDEHLESKNENKTMDFVDVMLEIMGSQETEYQIERSNIKALILDMLV 300

Query: 301 AAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVL 360
           AAMDTSAT IGWA+PELI+HPHVMKK+QDELE VVGLDR VEE+DL HLEYLDMV+KE+L
Sbjct: 301 AAMDTSATTIGWAIPELIKHPHVMKKMQDELEKVVGLDRKVEETDLDHLEYLDMVVKEIL 360

Query: 361 RLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERFISSQ 420
           RL+PP PLLVPHE+LEDCIVD F+IPKKSRIIVN WAIGR+P+ WIDPEKF+PERFI SQ
Sbjct: 361 RLHPPAPLLVPHEALEDCIVDGFYIPKKSRIIVNGWAIGRDPNFWIDPEKFFPERFIGSQ 420

Query: 421 VDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEF 480
           VDVRGRDFQLIPFG+GRRGCPG+Q+GL V+ LV+AQLVHCFDW+LPN  L  +LDMTEEF
Sbjct: 421 VDVRGRDFQLIPFGSGRRGCPGMQMGLTVVRLVIAQLVHCFDWELPNGTLPVKLDMTEEF 480

Query: 481 GLTCPRAQDLIAIPIYRLHN 501
           GLTCPRAQDL+  PIYRLHN
Sbjct: 481 GLTCPRAQDLMVTPIYRLHN 494

BLAST of Lag0013359 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-107
Identity = 203/509 (39.88%), Postives = 308/509 (60.51%), Query Frame = 0

Query: 13  LHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKFGPIMHMR 72
           L ++  LF    F ++ +    PPGP+G+PI G++ ++ Q  HR L  L+KK+G + H+R
Sbjct: 19  LVIVVSLFIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHLR 78

Query: 73  LGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSYWRNVRKM 132
           +G ++   VSSP+ A   L+  D VF++RP T+   +L+Y + ++ FA YG +WR +RK+
Sbjct: 79  MGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKV 138

Query: 133 CTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDMTCLMVFGR 192
           C +++ S  +  S+ S+R +E+  ++  V  +   G  I++  ++ +L  ++T    FG 
Sbjct: 139 CVMKVFSRKRAESWASVR-DEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRAAFG- 198

Query: 193 KFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMKNVSKVFDDFFEM 252
             +  E     F  ++QE  +L  A N+ D+IP+    D QG+ +R+       D F + 
Sbjct: 199 --SACEKGQDEFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDD 258

Query: 253 IIDEHLKSKDEERTKDFLDVM----------------------FDLMNSKDTEYRVDRSS 312
           IIDEH+K K+ +   D  DV+                       DL NS     ++ R +
Sbjct: 259 IIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNS----IKLTRDN 318

Query: 313 IKAIILDILGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLE 372
           IKAII+D++    +T A+ I WAL EL+R P  +K+VQ EL  VVGLDR VEESD+  L 
Sbjct: 319 IKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLT 378

Query: 373 YLDMVIKEVLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEK 432
           YL   +KE LR++PP+PLL+ HE+ ED  +D FFIPKKSR+++NA+AIGR+P++W DP+ 
Sbjct: 379 YLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDT 438

Query: 433 FYPERFISSQV-DVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDM 492
           F P RF+   V D +G +F+ IPFG+GRR CPG+QLGL  + L +A ++HCF WKLP+ M
Sbjct: 439 FRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGM 498

Query: 493 LAEELDMTEEFGLTCPRAQDLIAIPIYRL 499
              ELDM + FGLT P+A  L A+P  RL
Sbjct: 499 KPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of Lag0013359 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 370.2 bits (949), Expect = 2.7e-102
Identity = 205/506 (40.51%), Postives = 299/506 (59.09%), Query Frame = 0

Query: 6   ATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKF 65
           AT+  L+L + ++        +++   +LPPGP  +PI G+L  +   PHR L  +   +
Sbjct: 11  ATVLFLILRIFSHR------RNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTY 70

Query: 66  GPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 125
           GPI+H+RLG V+ +V +S   AE FLK HD  FASRP    +K ++Y  ++LVFA YG  
Sbjct: 71  GPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHR 130

Query: 126 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGV-AIDISSKVSSLITDM 185
           WR +RK+ ++ L S   +  FK +R+EE+G L    RE  + G   +++   V+  + + 
Sbjct: 131 WRLLRKISSVHLFSAKALEDFKHVRQEEVGTL---TRELVRVGTKPVNLGQLVNMCVVNA 190

Query: 186 TCLMVFGRKF--ADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFDLQGLTQRMKNV 245
               + GR+   AD +     F++M+ E+M L    N+GD++P +   DLQG+  +MK +
Sbjct: 191 LGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRL 250

Query: 246 SKVFDDFFEMIIDEH-LKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSS-----IKAIIL 305
            K FD F   I+ EH +  +D++ T    D++  L++ K T+   D  S     IKA++L
Sbjct: 251 HKRFDAFLSSILKEHEMNGQDQKHT----DMLSTLISLKGTDLDGDGGSLTDTEIKALLL 310

Query: 306 DILGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVI 365
           ++  A  DTSA+ + WA+ ELIRHP +M K Q+EL+ VVG DR V ESD+  L YL  VI
Sbjct: 311 NMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVI 370

Query: 366 KEVLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYPERF 425
           KE  RL+PP PL +PH + E C ++ + IPK S ++ N WAI R+P  W DP  F PERF
Sbjct: 371 KENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERF 430

Query: 426 I----SSQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAE 485
           +     S VDV+G DF+LIPFG GRR C G+ LGL  I  + A LV  FDW+L   +  E
Sbjct: 431 LPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPE 490

Query: 486 ELDMTEEFGLTCPRAQDLIAIPIYRL 499
           +L+M E +GLT  RA  L+  P  RL
Sbjct: 491 KLNMEESYGLTLQRAVPLVVHPKPRL 503

BLAST of Lag0013359 vs. TAIR 10
Match: AT3G48320.1 (cytochrome P450, family 71, subfamily A, polypeptide 21 )

HSP 1 Score: 367.1 bits (941), Expect = 2.3e-101
Identity = 184/491 (37.47%), Postives = 298/491 (60.69%), Query Frame = 0

Query: 7   TISLLLLHLLAYLFQTWPFNSKNKGKK--LPPGPKGFPIFGSLNLLKQFPHRDLHQLSKK 66
           ++++++L  L        F  + +GKK   P  P   P+ G+L+ L   PHR L  LS +
Sbjct: 3   SMTMIILQSLIIFITILFFKKQKRGKKSNTPRSPPRLPLIGNLHQLGHHPHRSLCSLSHR 62

Query: 67  FGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGS 126
           +GP+M + LG V  +VVSS   A   LKTHD VFASRP + + + L Y  +++ FA YG 
Sbjct: 63  YGPLMLLHLGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGE 122

Query: 127 YWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDM 186
           YWR ++ +C L LLSN  + SF+++R+EE+ L+++ +++++   + +++S  + SL  D+
Sbjct: 123 YWRQIKSVCVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSS--SLQVNVSELLGSLTNDV 182

Query: 187 TCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFD-LQGLTQRMKNVS 246
              +  GRK++     +   K +M+ +M L+   ++G Y+P++   D + GL  ++    
Sbjct: 183 ISRIALGRKYS----GETDSKELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTG 242

Query: 247 KVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILGAAM 306
              D+F E ++ +H+   D +RT DF+DV+  +   K   + +DR  IKAI+LD+L A  
Sbjct: 243 NDLDEFLEKVVQDHVDG-DGQRT-DFVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGT 302

Query: 307 DTSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLRLY 366
           D+S  ++ WA+ EL+RHP  ++ +Q+E+  +   +  V E D+ ++ YL  VIKE  RL+
Sbjct: 303 DSSYALMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLH 362

Query: 367 PPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAW-IDPEKFYPERFISSQVD 426
           PP+PLL PHES++D I+ D+ IP  +++++NAWAIGR  + W  D EKF PER + S VD
Sbjct: 363 PPLPLLAPHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLDSSVD 422

Query: 427 VRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFGL 486
            RG +F+L+PFG GRR CP I   +++I + LA  VH +DWKLP D    + ++ E  G+
Sbjct: 423 FRGHNFELVPFGAGRRICPAISFAVVLIEVALANFVHRYDWKLPEDSKENQTNVAESTGM 482

Query: 487 TCPRAQDLIAI 494
              R   L AI
Sbjct: 483 VIHRLFPLYAI 485

BLAST of Lag0013359 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 367.1 bits (941), Expect = 2.3e-101
Identity = 189/505 (37.43%), Postives = 307/505 (60.79%), Query Frame = 0

Query: 1   MAWIWATISLLLLHLLAYLFQTWPFNSKNKGKKLPPGPKGFPIFGSLNLLKQFPHRDLHQ 60
           M  +W    +LL+ +L    +    +SK +  + PP P G PI G+L+ L + PH+ L +
Sbjct: 1   MTVLWFVSLILLISILLVAVK----HSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCK 60

Query: 61  LSKKFGPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFA 120
           LSKK+GP+M ++LG V  ++VS+P+ A+  LK +DL   SRP    ++ LSY   ++ F+
Sbjct: 61  LSKKYGPVMLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFS 120

Query: 121 QYGSYWRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSL 180
           ++  YW+ +RK+C  EL  N +INS + +++ E+  LID + E+A     +++S    SL
Sbjct: 121 RFDDYWKELRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSL 180

Query: 181 ITDMTCLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFIS-IFD-LQGLTQR 240
             ++ C  VFG  F    L +  F+ ++ E ++++ + +  D+ P++  I D   GL  R
Sbjct: 181 NVNVICKAVFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHAR 240

Query: 241 MKNVSKVFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDT--EYRVDRSSIKAIIL 300
            +   +  D F+E +ID HL+   EE   DF+D++  L   +      ++ R+ IKAI++
Sbjct: 241 RERSVRDLDAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILM 300

Query: 301 DILGAAMDTSATVIGWALPELIRHPHVMKKVQDELENVVGLD---RMVEESDLIHLEYLD 360
           +IL   ++TSA  + WA+ ELIR+P VMKKVQ E+   +G +   R++   ++ HL YL+
Sbjct: 301 NILLGGINTSAITMTWAMAELIRNPRVMKKVQSEIRAQIGKNNKTRIISLDEINHLSYLN 360

Query: 361 MVIKEVLRLYPPVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAWIDPEKFYP 420
           MVIKE  RL+P  PLLVP E + +  ++ + I  K+R+ VN WAIGR+P  W DPE+F P
Sbjct: 361 MVIKETCRLHPVAPLLVPREVISEFKINGYTIQPKTRLHVNVWAIGRDPEIWKDPEEFLP 420

Query: 421 ERFISSQVDVRGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEE 480
           ERF+   +DV+G+D++L+PFG+GRR CP + +G+  +   LA L++ FDWKLP  +  E+
Sbjct: 421 ERFMDCDIDVKGQDYELLPFGSGRRICPAVYMGITTVEFGLANLLYHFDWKLPEGVAVED 480

Query: 481 LDMTEEFGLTCPRAQDLIAIPIYRL 499
           + M E  GLT  +  DL+ +P+  L
Sbjct: 481 IYMDEASGLTSHKKHDLLLVPVKSL 501

BLAST of Lag0013359 vs. TAIR 10
Match: AT3G48310.1 (cytochrome P450, family 71, subfamily A, polypeptide 22 )

HSP 1 Score: 365.5 bits (937), Expect = 6.6e-101
Identity = 185/490 (37.76%), Postives = 305/490 (62.24%), Query Frame = 0

Query: 8   ISLLLLHLLAYLFQTWPFNSKNKGKK--LPPGPKGFPIFGSLNLLKQFPHRDLHQLSKKF 67
           I ++LL L+ ++   + F  + KGKK   P  P   P+ G+L+ L + PHR L  LS ++
Sbjct: 5   IRIILLSLIIFITILF-FIKQKKGKKSNTPASPPRLPLIGNLHQLGRHPHRSLCSLSNRY 64

Query: 68  GPIMHMRLGLVNAIVVSSPQAAELFLKTHDLVFASRPLTMVSKFLSYGQKNLVFAQYGSY 127
           GP+M +R GLV  +VVSS   A   LKT+D VFASRP + + + + Y  +++  A YG Y
Sbjct: 65  GPLMLLRFGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEY 124

Query: 128 WRNVRKMCTLELLSNLKINSFKSMRKEELGLLIDYVREAAKDGVAIDISSKVSSLITDMT 187
           WR ++ +C L LL+N  + SF+++R+EE+ L+++ +++++   + +++S  + SL  D+ 
Sbjct: 125 WRQMKSVCVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSS--SLQVNLSELLGSLTNDVI 184

Query: 188 CLMVFGRKFADQELADRGFKAMMQEVMQLVAAPNLGDYIPFISIFD-LQGLTQRMKNVSK 247
             +  GRK++D    +  FK +M+ + +L+    +G Y+P+++  D + GL  ++K    
Sbjct: 185 SRVALGRKYSD----ETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGN 244

Query: 248 VFDDFFEMIIDEHLKSKDEERTKDFLDVMFDLMNSKDTEYRVDRSSIKAIILDILGAAMD 307
             D+F E ++ +H +  D +RT DF+DV+  +   K   + +DR SIKAIILD++    D
Sbjct: 245 DLDEFLEKVVQDH-EDGDAQRT-DFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTD 304

Query: 308 TSATVIGWALPELIRHPHVMKKVQDELENVVGLDRMVEESDLIHLEYLDMVIKEVLRLYP 367
           TS  ++ WA+ EL+  P  + ++Q+E+  +   +  V E D+  + YL  VIKE +RL+P
Sbjct: 305 TSYALMEWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLHP 364

Query: 368 PVPLLVPHESLEDCIVDDFFIPKKSRIIVNAWAIGRNPSAW-IDPEKFYPERFISSQVDV 427
           P+PL+VPHES +D  + D+ IP  +++++NAWAIGR  + W  D EKF PER ++S VD 
Sbjct: 365 PLPLMVPHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLNSSVDF 424

Query: 428 RGRDFQLIPFGTGRRGCPGIQLGLIVIHLVLAQLVHCFDWKLPNDMLAEELDMTEEFGLT 487
           RG +F+LIPFG GRR CP I   +I+I + LA LVH +DW+LP + + ++ ++ E  G+ 
Sbjct: 425 RGHNFELIPFGAGRRICPAISFAVILIEVTLANLVHRYDWRLPEEYIEDQTNVAESTGMV 484

Query: 488 CPRAQDLIAI 494
             R   L AI
Sbjct: 485 IHRLFPLYAI 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022950766.11.1e-24685.23cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_023543389.11.6e-24584.43cytochrome P450 CYP736A12-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038883676.16.0e-24584.40cytochrome P450 71AU50-like [Benincasa hispida][more]
XP_022978476.11.7e-24483.23cytochrome P450 CYP736A12-like [Cucurbita maxima][more]
XP_004143494.16.6e-24483.20cytochrome P450 CYP736A12 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A068Q5V64.2e-19365.73Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH181.1e-14353.70Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q50EK41.0e-11441.39Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
A0A068Q7215.6e-11342.39Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
P244651.4e-11141.29Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GFR15.3e-24785.23cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453773 PE=... [more]
A0A6J1IMS28.4e-24583.23cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478444 PE=3 ... [more]
A0A6J1BWX19.6e-24183.03cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005474 PE... [more]
A0A6J1BYU03.9e-21070.60cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
A0A5A7T3U28.8e-21071.00Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT4G36220.11.2e-10739.88ferulic acid 5-hydroxylase 1 [more]
AT5G07990.12.7e-10240.51Cytochrome P450 superfamily protein [more]
AT3G48320.12.3e-10137.47cytochrome P450, family 71, subfamily A, polypeptide 21 [more]
AT5G57260.12.3e-10137.43cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
AT3G48310.16.6e-10137.76cytochrome P450, family 71, subfamily A, polypeptide 22 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 62..81
score: 30.33
coord: 394..418
score: 34.68
coord: 180..198
score: 18.83
coord: 430..440
score: 53.76
coord: 290..307
score: 28.1
coord: 310..336
score: 37.5
coord: 440..463
score: 28.68
coord: 353..371
score: 40.56
IPR001128Cytochrome P450PRINTSPR00385P450coord: 431..440
score: 57.25
coord: 301..318
score: 31.15
coord: 354..365
score: 48.5
IPR001128Cytochrome P450PFAMPF00067p450coord: 35..482
e-value: 2.0E-103
score: 346.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 25..493
e-value: 1.5E-129
score: 434.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 35..497
NoneNo IPR availablePIRSRPIRSR000209-1PIRSR000209-1coord: 36..465
e-value: 2.8E-38
score: 129.0
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 1..499
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 1..499
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 433..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013359.1Lag0013359.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen