Lag0013353 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013353
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	E3 ubiquitin-protein ligase RHA2B-like
Location	chr1: 49594618 .. 49595339 (+)
RNA-Seq Expression	Lag0013353
Synteny	Lag0013353

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTTCATACAATGACCGCCGTCTTCTTCACGCTTTTACTTCTCGAATTCGTAATTCTCATTCGCACGATCTTCGGATTGAGGCCTAACGCCGACAAGCGCGTAATCACGACGGCGCAGTTTTTGAAACTGATCGAAGAGAAGAATCCGACGATTCGATTCTCCAATAAGATCACCTCAACTCCCGCCGATCAGTGCGCCGTTTGCCTCTCGGAATTCGAGGAAGGGGAAAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTCTTGCCGGACGATATTGTCGCCGGCTACCACAGGCTCCGGGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGATTTTTCTAGGGCGAAAGGCAATTTCATTTTGTCCATCACAGAATAGAATCAAATAACATAGAAAATGTTGATTTTCTGTAGATTAACTAGGGAATATTGTTTTCTCCCTTCAATTGACGAGCAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCTCCAGCTAGATCGAGTAAAATCGTGTATGTTTCTGTTTACCTGCAATTACGCAAGCCAACTGTTTGATGCTTTCTTTGCATGA

mRNA sequence

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTTCATACAATGACCGCCGTCTTCTTCACGCTTTTACTTCTCGAATTCGTAATTCTCATTCGCACGATCTTCGGATTGAGGCCTAACGCCGACAAGCGCGTAATCACGACGGCGCAGTTTTTGAAACTGATCGAAGAGAAGAATCCGACGATTCGATTCTCCAATAAGATCACCTCAACTCCCGCCGATCAGTGCGCCGTTTGCCTCTCGGAATTCGAGGAAGGGGAAAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTCTTGCCGGACGATATTGTCGCCGGCTACCACAGGCTCCGGGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGGAATATTGTTTTCTCCCTTCAATTGACGAGCAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCTCCAGCTAGATCGAGTAAAATCGTGTATGTTTCTGTTTACCTGCAATTACGCAAGCCAACTGTTTGATGCTTTCTTTGCATGA

Coding sequence (CDS)

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTTCATACAATGACCGCCGTCTTCTTCACGCTTTTACTTCTCGAATTCGTAATTCTCATTCGCACGATCTTCGGATTGAGGCCTAACGCCGACAAGCGCGTAATCACGACGGCGCAGTTTTTGAAACTGATCGAAGAGAAGAATCCGACGATTCGATTCTCCAATAAGATCACCTCAACTCCCGCCGATCAGTGCGCCGTTTGCCTCTCGGAATTCGAGGAAGGGGAAAAGGTACGGAAATTGCAATGCAACCACACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTGTTTCGCTACCTGTCCGCTTTGCCGGAGTAAGGTCTTGCCGGACGATATTGTCGCCGGCTACCACAGGCTCCGGGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTCATCTTTTTGTTATCGGCATTACATGGGAATATTGTTTTCTCCCTTCAATTGACGAGCAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCTCCAGCTAGATCGAGTAAAATCGTGTATGTTTCTGTTTACCTGCAATTACGCAAGCCAACTGTTTGATGCTTTCTTTGCATGA

Protein sequence

MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPTIRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVFSLQLTSNSVREQLVSGSIDLQLDRVKSCMFLFTCNYASQLFDAFFA

Homology

[Close]

BLAST of Lag0013353 vs. NCBI nr
Match: XP_022963436.1 (E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita moschata] >XP_023517189.1 E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita pepo subsp. pepo] >KAG7026818.1 E3 ubiquitin-protein ligase RHA2B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.5 bits (794), Expect = 1.1e-80
Identity = 149/158 (94.30%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLL+LE VIL+RTIFGLRPN DKRVI TAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLILELVILVRTIFGLRPNVDKRVIPTAQFLKLIEEKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNKITS+PADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKITSSPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIY 158

[Close]

BLAST of Lag0013353 vs. NCBI nr
Match: KAA0036272.1 (putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa] >TYK12666.1 putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa])

HSP 1 Score: 309.7 bits (792), Expect = 1.9e-80
Identity = 150/158 (94.94%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+T + ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSMY 158

[Close]

BLAST of Lag0013353 vs. NCBI nr
Match: XP_038883588.1 (E3 ubiquitin-protein ligase RHA2B-like [Benincasa hispida])

HSP 1 Score: 309.3 bits (791), Expect = 2.5e-80
Identity = 153/165 (92.73%), Postives = 158/165 (95.76%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRV+TTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVVTTAQFLKLIEEKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+TS+ ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKLTSSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHG-NIVFSLQLTS 165
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHG  ++F   L S
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGVELIFMYLLQS 165

[Close]

BLAST of Lag0013353 vs. NCBI nr
Match: XP_022132528.1 (E3 ubiquitin-protein ligase RHA2B-like [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 7.2e-80
Identity = 146/158 (92.41%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRP ADKRVITT Q+ KLIEE+NPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPGADKRVITTTQYFKLIEERNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+TS+PADQCAVCLSEFEEG+KVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKLTSSPADQCAVCLSEFEEGDKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDD+VAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDVVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIY 158

[Close]

BLAST of Lag0013353 vs. NCBI nr
Match: XP_008440648.1 (PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo])

HSP 1 Score: 304.3 bits (778), Expect = 7.9e-79
Identity = 148/153 (96.73%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+T + ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-14
Identity = 41/101 (40.59%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 22  LLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPTIRFSNKITSTPADQCAVCLSE 81
           LL  F   +R++  L P++   V+ T+    L ++ N    FS + +   A  C VCLS+
Sbjct: 22  LLATFFRHVRSLL-LFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVCLSK 81

Query: 82  FEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLP 123
            + GE+VRKL C H FHK CL+ WL+     CPLCRS +LP
Sbjct: 82  LKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 75  CAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLPDDIVAGYHR 132
           C VCLS+ +EGE+VRKL+C H FHK CL+ WL     TCPLCRS ++ DD V+   R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 28/66 (42.42%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 59  PTIRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRS 118
           PT++ +  +  +  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

[Close]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.3e-10
Identity = 34/77 (44.16%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 53  LIEEKNPTIRFSNKITSTPAD---QCAVCLSEFEEGEKVRKLQ-CNHTFHKDCLDNWLKL 112
           LI E  P I+F  ++T++  D    CAVCL EFE  +++R L+ C H FH+ CLD W+  
Sbjct: 70  LIREILPVIKF-EELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDH 129

Query: 113 CFATCPLCRSKVLPDDI 126
              TCPLCR+  +PD++
Sbjct: 130 DQKTCPLCRTPFVPDEM 145

[Close]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot
Match: Q0VD51 (E3 ubiquitin-protein ligase RNF13 OS=Bos taurus OX=9913 GN=RNF13 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.3e-10
Identity = 25/50 (50.00%), Postives = 36/50 (72.00%), Query Frame = 0


Query: 73  DQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLP 123
           D CA+CL E+E+G+K+R L C+H +H  C+D WL     TCP+C+ KV+P
Sbjct: 238 DVCAICLDEYEDGDKLRILPCSHAYHCKCVDPWLTKTKKTCPVCKQKVVP 287

[Close]

BLAST of Lag0013353 vs. ExPASy TrEMBL
Match: A0A6J1HG49 (E3 ubiquitin-protein ligase RHA2B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463642 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 5.4e-81
Identity = 149/158 (94.30%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLL+LE VIL+RTIFGLRPN DKRVI TAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLILELVILVRTIFGLRPNVDKRVIPTAQFLKLIEEKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNKITS+PADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKITSSPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIY 158

[Close]

BLAST of Lag0013353 vs. ExPASy TrEMBL
Match: A0A5A7SYH1 (Putative E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002540 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 9.1e-81
Identity = 150/158 (94.94%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+T + ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSMY 158

[Close]

BLAST of Lag0013353 vs. ExPASy TrEMBL
Match: A0A0A0KM08 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.5e-80
Identity = 148/158 (93.67%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+T + ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIY 158

[Close]

BLAST of Lag0013353 vs. ExPASy TrEMBL
Match: A0A6J1BWI1 (E3 ubiquitin-protein ligase RHA2B-like OS=Momordica charantia OX=3673 GN=LOC111005363 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.5e-80
Identity = 146/158 (92.41%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRP ADKRVITT Q+ KLIEE+NPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPGADKRVITTTQYFKLIEERNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+TS+PADQCAVCLSEFEEG+KVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKLTSSPADQCAVCLSEFEEGDKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNIVF 159
           LPDD+VAGYHRLRDRVEYDGSDEELIFLLSALHGN ++
Sbjct: 121 LPDDVVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIY 158

[Close]

BLAST of Lag0013353 vs. ExPASy TrEMBL
Match: A0A1S3B160 (probable E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo OX=3656 GN=LOC103485001 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.8e-79
Identity = 148/153 (96.73%), Postives = 151/153 (98.69%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           IRFSNK+T + ADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of Lag0013353 vs. TAIR 10
Match: AT2G01150.1 (RING-H2 finger protein 2B )

HSP 1 Score: 80.9 bits (198), Expect = 1.3e-15
Identity = 41/101 (40.59%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 22  LLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEEKNPTIRFSNKITSTPADQCAVCLSE 81
           LL  F   +R++  L P++   V+ T+    L ++ N    FS + +   A  C VCLS+
Sbjct: 22  LLATFFRHVRSLL-LFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVCLSK 81

Query: 82  FEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLP 123
            + GE+VRKL C H FHK CL+ WL+     CPLCRS +LP
Sbjct: 82  LKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Lag0013353 vs. TAIR 10
Match: AT1G15100.1 (RING-H2 finger A2A )

HSP 1 Score: 77.0 bits (188), Expect = 1.9e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 75  CAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKVLPDDIVAGYHR 132
           C VCLS+ +EGE+VRKL+C H FHK CL+ WL     TCPLCRS ++ DD V+   R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of Lag0013353 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-12
Identity = 28/66 (42.42%), Postives = 45/66 (68.18%), Query Frame = 0


Query: 59  PTIRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRS 118
           PT++ +  +  +  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

[Close]

BLAST of Lag0013353 vs. TAIR 10
Match: AT5G45290.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.8e-12
Identity = 29/70 (41.43%), Postives = 38/70 (54.29%), Query Frame = 0


Query: 61  IRFSNKITSTPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120
           I+   K  S    QC +CL E+EE + +R L C+H FHK C+D WLK     CPLCR  +
Sbjct: 476 IKLYTKSQSEDPSQCYICLVEYEEADSIRTLPCHHEFHKTCVDKWLKEIHRVCPLCRGDI 535

Query: 121 LPDDIVAGYH 131
              D  +  H
Sbjct: 536 CRHDPSSEQH 545

[Close]

BLAST of Lag0013353 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 68.2 bits (165), Expect = 8.9e-12
Identity = 34/77 (44.16%), Postives = 49/77 (63.64%), Query Frame = 0


Query: 53  LIEEKNPTIRFSNKITSTPAD---QCAVCLSEFEEGEKVRKLQ-CNHTFHKDCLDNWLKL 112
           LI E  P I+F  ++T++  D    CAVCL EFE  +++R L+ C H FH+ CLD W+  
Sbjct: 70  LIREILPVIKF-EELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDH 129

Query: 113 CFATCPLCRSKVLPDDI 126
              TCPLCR+  +PD++
Sbjct: 130 DQKTCPLCRTPFVPDEM 145

The following BLAST results are available for this feature:

BLAST of Lag0013353 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022963436.1	1.1e-80	94.30	E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita moschata] >XP_02351...	[more]
KAA0036272.1	1.9e-80	94.94	putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa] >TYK12666....	[more]
XP_038883588.1	2.5e-80	92.73	E3 ubiquitin-protein ligase RHA2B-like [Benincasa hispida]	[more]
XP_022132528.1	7.2e-80	92.41	E3 ubiquitin-protein ligase RHA2B-like [Momordica charantia]	[more]
XP_008440648.1	7.9e-79	96.73	PREDICTED: probable E3 ubiquitin-protein ligase RHA2B [Cucumis melo]	[more]

BLAST of Lag0013353 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZU51	1.9e-14	40.59	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]
Q9ZT50	2.7e-13	56.14	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 ...	[more]
Q8LPN7	1.5e-11	42.42	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9XF92	1.3e-10	44.16	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q0VD51	1.3e-10	50.00	E3 ubiquitin-protein ligase RNF13 OS=Bos taurus OX=9913 GN=RNF13 PE=2 SV=1	[more]

BLAST of Lag0013353 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HG49	5.4e-81	94.30	E3 ubiquitin-protein ligase RHA2B-like isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A5A7SYH1	9.1e-81	94.94	Putative E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0KM08	3.5e-80	93.67	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G501220 P...	[more]
A0A6J1BWI1	3.5e-80	92.41	E3 ubiquitin-protein ligase RHA2B-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A1S3B160	3.8e-79	96.73	probable E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo OX=3656 GN=LOC1034850...	[more]

BLAST of Lag0013353 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01150.1	1.3e-15	40.59	RING-H2 finger protein 2B	[more]
AT1G15100.1	1.9e-14	56.14	RING-H2 finger A2A	[more]
AT3G19950.1	1.1e-12	42.42	RING/U-box superfamily protein	[more]
AT5G45290.1	1.8e-12	41.43	RING/U-box superfamily protein	[more]
AT3G61460.1	8.9e-12	44.16	brassinosteroid-responsive RING-H2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..116 e-value: 1.5E-6 score: 37.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 73..117 e-value: 9.6E-13 score: 48.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 75..117 score: 12.725326
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 53..124 e-value: 4.6E-20 score: 72.6
None	No IPR available	PANTHER	PTHR47662:SF1	E3 UBIQUITIN-PROTEIN LIGASE XERICO-RELATED	coord: 1..157
None	No IPR available	PANTHER	PTHR47662	FAMILY NOT NAMED	coord: 1..157
None	No IPR available	CDD	cd16486	mRING-H2-C3H2C2D_ZSWM2	coord: 74..117 e-value: 4.68613E-19 score: 74.9557
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 74..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013353.1	Lag0013353.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane