Lag0013338 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013338
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPhosphoglycerate mutase (2,3-diphosphoglycerate-dependent)
Locationchr1: 49476229 .. 49477090 (-)
RNA-Seq ExpressionLag0013338
SyntenyLag0013338
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGAATGAGAAGAATTTATTCACTGGCTCTGTTGATGTACCTTTAACAAGGAGAGGTGTAGAGGAAGTAGTTGAAGCTGGTAAGAGGATTAGCAACGTACTGATAGACCTCGCTTACACTTCTGCACTGATTCACTTGCAAATGACAGGTGTGCTTGCCTTTGACTCAACATTGCTGCAAAAAGGTATCTATCTTGTAAGAGTGGATAGGGTTTGATTAGCTTCTAGTCCCGACTTTGATGAATTCATAACTCCATACTAGTGTTGCTCAAGATGGTAGCAAGTTTATAATAGAGAAGAAAGCATCTGAAAATTCCTTTCCTTCCCCAAAAGGCATTCTTATCATTGGATGAGAAGACTTTGCTGTTTAGAATACAGAGTTCAAACCAATATTTACAGGAAAAAAATTATATAATGCCTCTGTGAAGATCAAGAGTCAAGACAAGTTTGAGTTGAAGCGTATGGTGGGTAAAAACTTTTCTTTCTTTAAATCAAAAGAAATAGTATAAGAATAATAAACTTAACCCTCCGAGCTAGATTGAAGTTTTGATCATTATTTTTTAAACATCCCATAATTAACAGGAAAGAGATATTTCAAATTTAGACCATCCTATGCTTTCAGGTTCCTATCATTATGCATAATTGTGATAAACAGGCAGAGGCATGGAGTCAAATTTACAATGGTGAAGCTAAAAAGCAATCAGTGCTGGTTATTAGAGCTTGGCAATTGAATGAAAGAATGTACTCTCTCTTTTCACGGGGATTTATACTTTCCTTCTATCTGAAGACTAGATTAGTTATTTTGATGCACAAATATATGATGCAGGTATGGTGAGCTACAAGGACTGAATAAACAGTAG

mRNA sequence

ATGAGGAATGAGAAGAATTTATTCACTGGCTCTGTTGATGTACCTTTAACAAGGAGAGGTGTAGAGGAAGTAGTTGAAGCTGGTAAGAGGATTAGCAACGTACTGATAGACCTCGCTTACACTTCTGCACTGATTCACTTGCAAATGACAGGTGTGCTTGCCTTTGACTCAACATTGCTGCAAAAAGGAAAAAAATTATATAATGCCTCTGTGAAGATCAAGAGTCAAGACAAGTTTGAGTTGAAGCGTATGGTTCCTATCATTATGCATAATTGTGATAAACAGGCAGAGGCATGGAGTCAAATTTACAATGGTGAAGCTAAAAAGCAATCAGTGCTGGTTATTAGAGCTTGGCAATTGAATGAAAGAATGTATGGTGAGCTACAAGGACTGAATAAACAGTAG

Coding sequence (CDS)

ATGAGGAATGAGAAGAATTTATTCACTGGCTCTGTTGATGTACCTTTAACAAGGAGAGGTGTAGAGGAAGTAGTTGAAGCTGGTAAGAGGATTAGCAACGTACTGATAGACCTCGCTTACACTTCTGCACTGATTCACTTGCAAATGACAGGTGTGCTTGCCTTTGACTCAACATTGCTGCAAAAAGGAAAAAAATTATATAATGCCTCTGTGAAGATCAAGAGTCAAGACAAGTTTGAGTTGAAGCGTATGGTTCCTATCATTATGCATAATTGTGATAAACAGGCAGAGGCATGGAGTCAAATTTACAATGGTGAAGCTAAAAAGCAATCAGTGCTGGTTATTAGAGCTTGGCAATTGAATGAAAGAATGTATGGTGAGCTACAAGGACTGAATAAACAGTAG

Protein sequence

MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNERMYGELQGLNKQ
Homology
[Close]
BLAST of Lag0013338 vs. NCBI nr
Match: XP_038883083.1 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2-like [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 3.2e-36
Identity = 87/134 (64.93%), Postives = 93/134 (69.40%), Query Frame = 0 

Query: 1   MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLL 60
           M NEKNLFTGSVDVPLTRRG+EE +EAGKRI N+ +D  YTSALI  QMT VLA      
Sbjct: 102 MWNEKNLFTGSVDVPLTRRGIEEAIEAGKRICNIPLDFVYTSALIRSQMTAVLALTQHRC 161

Query: 61  QKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQL 120
           +K                      VPIIMHNC KQAEAWSQIY+ EAKKQS  VIRAWQL
Sbjct: 162 KK----------------------VPIIMHNCSKQAEAWSQIYSDEAKKQSFPVIRAWQL 213

Query: 121 NERMYGELQGLNKQ 135
           NERMYGELQGLNKQ
Sbjct: 222 NERMYGELQGLNKQ 213

[Close]
BLAST of Lag0013338 vs. NCBI nr
Match: XP_023004021.1 (uncharacterized protein LOC111497455 isoform X5 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 90/134 (67.16%), Postives = 97/134 (72.39%), Query Frame = 0 

Query: 1   MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLL 60
           M NEKNLFTGSVDVPLTRRGVEE +EAGKRISN+ ID  YTSALI  QMT VLA    L+
Sbjct: 102 MWNEKNLFTGSVDVPLTRRGVEEAIEAGKRISNLPIDYVYTSALIRSQMTAVLA----LI 161

Query: 61  QKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQL 120
           Q   K                   VPII+HNC+KQAEAWSQIY+ EA+KQS  VIRAWQL
Sbjct: 162 QHRCK------------------KVPIIIHNCNKQAEAWSQIYSDEARKQSFPVIRAWQL 213

Query: 121 NERMYGELQGLNKQ 135
           NERMYGELQGLNKQ
Sbjct: 222 NERMYGELQGLNKQ 213

[Close]
BLAST of Lag0013338 vs. NCBI nr
Match: XP_023004020.1 (uncharacterized protein LOC111497455 isoform X4 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 90/134 (67.16%), Postives = 97/134 (72.39%), Query Frame = 0 

Query: 1   MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLL 60
           M NEKNLFTGSVDVPLTRRGVEE +EAGKRISN+ ID  YTSALI  QMT VLA    L+
Sbjct: 104 MWNEKNLFTGSVDVPLTRRGVEEAIEAGKRISNLPIDYVYTSALIRSQMTAVLA----LI 163

Query: 61  QKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQL 120
           Q   K                   VPII+HNC+KQAEAWSQIY+ EA+KQS  VIRAWQL
Sbjct: 164 QHRCK------------------KVPIIIHNCNKQAEAWSQIYSDEARKQSFPVIRAWQL 215

Query: 121 NERMYGELQGLNKQ 135
           NERMYGELQGLNKQ
Sbjct: 224 NERMYGELQGLNKQ 215

[Close]
BLAST of Lag0013338 vs. NCBI nr
Match: XP_023004022.1 (uncharacterized protein LOC111497455 isoform X6 [Cucurbita maxima] >XP_023004023.1 uncharacterized protein LOC111497455 isoform X6 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 90/134 (67.16%), Postives = 97/134 (72.39%), Query Frame = 0 

Query: 1   MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLL 60
           M NEKNLFTGSVDVPLTRRGVEE +EAGKRISN+ ID  YTSALI  QMT VLA    L+
Sbjct: 172 MWNEKNLFTGSVDVPLTRRGVEEAIEAGKRISNLPIDYVYTSALIRSQMTAVLA----LI 231

Query: 61  QKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQL 120
           Q   K                   VPII+HNC+KQAEAWSQIY+ EA+KQS  VIRAWQL
Sbjct: 232 QHRCK------------------KVPIIIHNCNKQAEAWSQIYSDEARKQSFPVIRAWQL 283

Query: 121 NERMYGELQGLNKQ 135
           NERMYGELQGLNKQ
Sbjct: 292 NERMYGELQGLNKQ 283

[Close]
BLAST of Lag0013338 vs. NCBI nr
Match: XP_023004019.1 (uncharacterized protein LOC111497455 isoform X3 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 9.3e-36
Identity = 90/134 (67.16%), Postives = 97/134 (72.39%), Query Frame = 0 

Query: 1   MRNEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLL 60
           M NEKNLFTGSVDVPLTRRGVEE +EAGKRISN+ ID  YTSALI  QMT VLA    L+
Sbjct: 172 MWNEKNLFTGSVDVPLTRRGVEEAIEAGKRISNLPIDYVYTSALIRSQMTAVLA----LI 231

Query: 61  QKGKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQL 120
           Q   K                   VPII+HNC+KQAEAWSQIY+ EA+KQS  VIRAWQL
Sbjct: 232 QHRCK------------------KVPIIIHNCNKQAEAWSQIYSDEARKQSFPVIRAWQL 283

Query: 121 NERMYGELQGLNKQ 135
           NERMYGELQGLNKQ
Sbjct: 292 NERMYGELQGLNKQ 283

[Close]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match: Q9LM13 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 1 OS=Arabidopsis thaliana OX=3702 GN=gpmA1 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-31
Identity = 72/132 (54.55%), Postives = 91/132 (68.94%), Query Frame = 0 

Query: 3   NEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQK 62
           NEKNLFTG VDVPLT +GVEE +EAGKRISN+ +D+ +TS+LI  QMT +LA    ++Q 
Sbjct: 91  NEKNLFTGCVDVPLTEKGVEEAIEAGKRISNIPVDVIFTSSLIRAQMTAMLA----MIQH 150

Query: 63  GKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNE 122
            +K                   VPII+H+  +QA+ WSQ+++ E K QS+ VI AWQLNE
Sbjct: 151 RRK------------------KVPIILHDESEQAKTWSQVFSDETKNQSIPVIPAWQLNE 200

Query: 123 RMYGELQGLNKQ 135
           RMYGELQGLNKQ
Sbjct: 211 RMYGELQGLNKQ 200

[Close]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match: F4I8M8 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 OS=Arabidopsis thaliana OX=3702 GN=gpmA2 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 67/132 (50.76%), Postives = 87/132 (65.91%), Query Frame = 0 

Query: 3   NEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQK 62
           NEKNLFTG VDVPLT++GV E +EAGK+ISN+ +DL +TS+LI  QMT +LA        
Sbjct: 92  NEKNLFTGCVDVPLTQKGVGEAIEAGKKISNIPVDLIFTSSLIRAQMTAMLAMTQ----- 151

Query: 63  GKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNE 122
                              ++ VPII+HN   +A+ WS +++ E +KQS+ VI AWQLNE
Sbjct: 152 -----------------HRRKKVPIILHNESVKAKTWSHVFSEETRKQSIPVIAAWQLNE 201

Query: 123 RMYGELQGLNKQ 135
           RMYGELQGLNK+
Sbjct: 212 RMYGELQGLNKK 201

[Close]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match: Q6MEW4 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=gpmA PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.6e-25
Identity = 61/132 (46.21%), Postives = 85/132 (64.39%), Query Frame = 0 

Query: 3   NEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQK 62
           N  NLFTG VD+PL+ +G+EE +EAGK+I N  IDL +TS+LI  QMT +LA   ++   
Sbjct: 15  NLANLFTGWVDIPLSFKGIEEAIEAGKQIKNYPIDLIFTSSLIRAQMTAMLAM--SVHTS 74

Query: 63  GKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNE 122
           GK                    VP+I+H  + + E W+ IY+ E++ Q++ VIRAW+LNE
Sbjct: 75  GK--------------------VPVILHTGEGRLEEWASIYSSESQSQTIPVIRAWELNE 124

Query: 123 RMYGELQGLNKQ 135
           RMYGELQG+NK+
Sbjct: 135 RMYGELQGINKE 124

[Close]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match: Q9Z743 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Chlamydia pneumoniae OX=83558 GN=gpmA PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-16
Identity = 50/132 (37.88%), Postives = 75/132 (56.82%), Query Frame = 0 

Query: 3   NEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQK 62
           NEKNLF+G VD+PL+++G+EE   AG+ I N+ ID  +TS L+   MT +LA  +   +K
Sbjct: 15  NEKNLFSGWVDIPLSQQGIEEAFSAGRAIQNLPIDCIFTSTLVRSLMTALLAMTNHHSKK 74

Query: 63  GKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNE 122
                                 +P I+H  D +A+  S+IY+ E +   + + ++  LNE
Sbjct: 75  ----------------------IPYIVHE-DPKAKEMSRIYSAEEENNMIPLYQSSALNE 123

Query: 123 RMYGELQGLNKQ 135
           RMYGELQG NK+
Sbjct: 135 RMYGELQGKNKK 123

[Close]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match: Q821N6 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Chlamydophila caviae (strain ATCC VR-813 / DSM 19441 / GPIC) OX=227941 GN=gpmA PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 2.9e-16
Identity = 53/132 (40.15%), Postives = 81/132 (61.36%), Query Frame = 0 

Query: 3   NEKNLFTGSVDVPLTRRGVEEVVEAGKRISNVLIDLAYTSALIHLQMTGVLAFDSTLLQK 62
           NEKNLFTG VD+PL+++G++E + AG+ I ++ ID  +TS+L+   MT +LA        
Sbjct: 15  NEKNLFTGWVDIPLSQQGIDEAIHAGQVIKDLPIDCIFTSSLVRSLMTALLAM------- 74

Query: 63  GKKLYNASVKIKSQDKFELKRMVPIIMHNCDKQAEAWSQIYNGEAKKQSVLVIRAWQLNE 122
               +++S KI            P I+H+ D+Q +  S+IY+ E +K  + + R+  LNE
Sbjct: 75  ---THHSSKKI------------PYIIHD-DEQQKLMSRIYSDE-EKSMIPLYRSSALNE 122

Query: 123 RMYGELQGLNKQ 135
           RMYGELQG NK+
Sbjct: 135 RMYGELQGKNKK 122

The following BLAST results are available for this feature:
BLAST of Lag0013338 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038883083.13.2e-3664.932,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2-like [Benincasa hisp... [more]
XP_023004021.19.3e-3667.16uncharacterized protein LOC111497455 isoform X5 [Cucurbita maxima][more]
XP_023004020.19.3e-3667.16uncharacterized protein LOC111497455 isoform X4 [Cucurbita maxima][more]
XP_023004022.19.3e-3667.16uncharacterized protein LOC111497455 isoform X6 [Cucurbita maxima] >XP_023004023... [more]
XP_023004019.19.3e-3667.16uncharacterized protein LOC111497455 isoform X3 [Cucurbita maxima][more]
BLAST of Lag0013338 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LM132.5e-3154.552,3-bisphosphoglycerate-dependent phosphoglycerate mutase 1 OS=Arabidopsis thali... [more]
F4I8M81.4e-2950.762,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 OS=Arabidopsis thali... [more]
Q6MEW42.6e-2546.212,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Protochlamydia amoe... [more]
Q9Z7431.7e-1637.882,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Chlamydia pneumonia... [more]
Q821N62.9e-1640.152,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Chlamydophila cavia... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013078Histidine phosphatase superfamily, clade-1PFAMPF00300His_Phos_1coord: 3..48
e-value: 9.1E-5
score: 22.2
IPR029033Histidine phosphatase superfamilyGENE3D3.40.50.1240coord: 1..134
e-value: 3.8E-15
score: 58.1
IPR029033Histidine phosphatase superfamilySUPERFAMILY53254Phosphoglycerate mutase-likecoord: 3..133
NoneNo IPR availablePANTHERPTHR11931:SF16PHOSPHOGLYCERATE/BISPHOSPHOGLYCERATE MUTASE FAMILY PROTEINcoord: 1..57
coord: 82..134
IPR005952Phosphoglycerate mutase 1PANTHERPTHR11931PHOSPHOGLYCERATE MUTASEcoord: 1..57
coord: 82..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013338.1Lag0013338.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0016020 membrane
molecular_function GO:0004619 phosphoglycerate mutase activity
molecular_function GO:0016868 intramolecular transferase activity, phosphotransferases