Lag0013310 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0013310
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr1: 49237265 .. 49238292 (-)
RNA-Seq ExpressionLag0013310
SyntenyLag0013310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGAGCCTCGGGCAGTTTGCCGAACCAATTTACAGTTCCCTTGGTGGTTTCCACTTGCGCGGAACTAATGATGCTCAACCATGGTATGAGCATTCACGGGTTGGCATTGAAACTTGGGCTTTTTGCTGGTAATCTGCTGTTGGTTCTTCTTTGGTTTCCATGTATTCGAAATGTGGTCACCCAGAAAGTGCATCTCTTCTGTTCGATGAAATTACTGTTAAAGATGTGGTTGCTTGGACTGCCCTTATAGTTGGCCACGTCCAGAGTAATGAGAGTGAGAAGGGTTTGAAATGTCTTTATGAGATGCATAGGAATGGAGAGACCCCGAATTATAGAACATTAGGAGGCGGGTTTCAAGCTTGTGTTGATATGGATGCTTTAGTAGAGGCCAGGTGTTTACATGGTTTGGCTTTAAAAAGTGGAATTCTGTGTTTTGAAGTCGTTAGATCTTCCATTCTCTCTATGTACTCAAGGTGTGGGTCACCTGAAGAAGCTTACTGTTGCTTTTGTAAATTAATTCAATAAGACCTTATCTCTGGTCTTATCTCTTGGACATCAATTATTTCTGTTCACGCTAAATTTGGGTTAATGACTGAATGTCTACATTTATGTTGGGAAATGCTGGCCGGTGAAATAATCCCAGATGAAATTACGATCAGTTGCATGCTTTTGGGTTTTGGTAATTCTGATAGAATCTCTGAAGGAAAAGCCTTTCATGGTTGGATTCCGAGACAATGTTGTGCAGGGAGTGGAGTAGCTCAGGATGCAATACTCTCCATGTACTGTAAGTTGGGACTCTTAAGCACTGCAGAGAAGATCTTTCATAGTTTACATAAGAGCAGCAAAGAAGATTGGAACACTATGATGTTAGGATATAGCAATACGGGGCAGAAAGAAAAGTGTATAGACTTTTTCAGGGAGATGCAACTTTTAGGCATAGAACCTGATTTGATCAGTTTAGTTTCTGTCATTTCTTCATGTTCACAAGTTGGAGCTATCAATATTGGTCGGTCCGTTCACTGCTGA

mRNA sequence

ATGCGAGCCTCGGGCAGTTTGCCGAACCAATTTACAGTTCCCTTGGTGGTTTCCACTTGCGCGGAACTAATGATGCTCAACCATGGTATGAGCATTCACGGGTTGGCATTGAAACTTGGGCTTTTTGCTGAAAGTGCATCTCTTCTGTTCGATGAAATTACTGTTAAAGATGTGGTTGCTTGGACTGCCCTTATAGTTGGCCACGTCCAGAGTAATGAGAGTGAGAAGGGTTTGAAATGTCTTTATGAGATGCATAGGAATGGAGAGACCCCGAATTATAGAACATTAGGAGGCGGGTTTCAAGCTTGTGTTGATATGGATGCTTTAGTAGAGGCCAGAATCTCTGAAGGAAAAGCCTTTCATGGTTGGATTCCGAGACAATGTTGTGCAGGGAGTGGAGTAGCTCAGGATGCAATACTCTCCATGTACTGTAAGTTGGGACTCTTAAGCACTGCAGAGAAGATCTTTCATAGTTTACATAAGAGCAGCAAAGAAGATTGGAACACTATGATGTTAGGATATAGCAATACGGGGCAGAAAGAAAAGTGTATAGACTTTTTCAGGGAGATGCAACTTTTAGGCATAGAACCTGATTTGATCAGTTTAGTTTCTGTCATTTCTTCATGTTCACAAGTTGGAGCTATCAATATTGGTCGGTCCGTTCACTGCTGA

Coding sequence (CDS)

ATGCGAGCCTCGGGCAGTTTGCCGAACCAATTTACAGTTCCCTTGGTGGTTTCCACTTGCGCGGAACTAATGATGCTCAACCATGGTATGAGCATTCACGGGTTGGCATTGAAACTTGGGCTTTTTGCTGAAAGTGCATCTCTTCTGTTCGATGAAATTACTGTTAAAGATGTGGTTGCTTGGACTGCCCTTATAGTTGGCCACGTCCAGAGTAATGAGAGTGAGAAGGGTTTGAAATGTCTTTATGAGATGCATAGGAATGGAGAGACCCCGAATTATAGAACATTAGGAGGCGGGTTTCAAGCTTGTGTTGATATGGATGCTTTAGTAGAGGCCAGAATCTCTGAAGGAAAAGCCTTTCATGGTTGGATTCCGAGACAATGTTGTGCAGGGAGTGGAGTAGCTCAGGATGCAATACTCTCCATGTACTGTAAGTTGGGACTCTTAAGCACTGCAGAGAAGATCTTTCATAGTTTACATAAGAGCAGCAAAGAAGATTGGAACACTATGATGTTAGGATATAGCAATACGGGGCAGAAAGAAAAGTGTATAGACTTTTTCAGGGAGATGCAACTTTTAGGCATAGAACCTGATTTGATCAGTTTAGTTTCTGTCATTTCTTCATGTTCACAAGTTGGAGCTATCAATATTGGTCGGTCCGTTCACTGCTGA

Protein sequence

MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLFAESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQACVDMDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKSSKEDWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSVHC
Homology
BLAST of Lag0013310 vs. NCBI nr
Match: KAG7010985.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335.5 bits (859), Expect = 3.5e-88
Identity = 186/338 (55.03%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFT+P+VVSTCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 51  MRASSSLPNQFTIPMVVSTCAELMMLNHGMNIHGLALKLGLFVGNSAVGSSLIYMYSKCG 110

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
            AESASL+F+EITVKDVVAWTALI+G+VQ+NESEKGLKCL+EMHRNG TPNYRT+GGGFQ
Sbjct: 111 NAESASLMFNEITVKDVVAWTALIIGYVQNNESEKGLKCLFEMHRNGCTPNYRTIGGGFQ 170

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 171 ACVDLDALVEGRCLHGLALKSGFLCFEVVKSSILSMYSRCGSPEEAYRCFFKLEQKDLIS 230

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          RISEGKAFH WI 
Sbjct: 231 WTSIIAVHSKLGLMSECLHLFWEMQASGIIPDDIVISCMLLGFGNFDRISEGKAFHAWIL 290

BLAST of Lag0013310 vs. NCBI nr
Match: KAG6571177.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.5 bits (859), Expect = 3.5e-88
Identity = 186/338 (55.03%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFT+P+VVSTCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 51  MRASSSLPNQFTIPMVVSTCAELMMLNHGMNIHGLALKLGLFVGNSAVGSSLIYMYSKCG 110

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
            AESASL+F+EITVKDVVAWTALI+G+VQ+NESEKGLKCL+EMHRNG TPNYRT+GGGFQ
Sbjct: 111 NAESASLMFNEITVKDVVAWTALIIGYVQNNESEKGLKCLFEMHRNGCTPNYRTIGGGFQ 170

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 171 ACVDLDALVEGRCLHGLALKSGFLCFEVVKSSILSMYSRCGSPEEAYRCFFKLEQKDLIS 230

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          RISEGKAFH WI 
Sbjct: 231 WTSIIAVHSKLGLMSECLHLFWEMQASGIIPDDIVISCMLLGFGNFDRISEGKAFHAWIL 290

BLAST of Lag0013310 vs. NCBI nr
Match: XP_022986468.1 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 333.6 bits (854), Expect = 1.3e-87
Identity = 185/338 (54.73%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFT+P+VVSTCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 122 MRASSSLPNQFTIPMVVSTCAELMMLNHGMNIHGLALKLGLFVGNSAVGSSLIYMYSKCG 181

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
            AESASL+F++ITVKDVVAWTALI+G+VQ+NESEKGLKCL+EMHRNG TPNYRT+GGGFQ
Sbjct: 182 NAESASLMFNDITVKDVVAWTALIIGYVQNNESEKGLKCLFEMHRNGCTPNYRTIGGGFQ 241

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD++ALVE                                                  
Sbjct: 242 ACVDLEALVEGRCLHGLALKSGFLCFEVVKSSILSMYSRCGSPEEAYRCFFKLEQKDLIS 301

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          RISEGKAFH WI 
Sbjct: 302 WTSIMAVHSKLGLMSECLHLFWEMQASGIIPDDIVISCMLLGFGNFDRISEGKAFHAWIL 361

BLAST of Lag0013310 vs. NCBI nr
Match: XP_022154323.1 (pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 332.8 bits (852), Expect = 2.3e-87
Identity = 185/338 (54.73%), Postives = 201/338 (59.47%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFTVP+V+STCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 1   MRASSSLPNQFTVPMVLSTCAELMMLNHGMNIHGLALKLGLFYGNSAVGSSLVYMYSKCG 60

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
             ESASLLFDEIT+KDVVAWTALIVGHVQ+NESEKGLKCL++MHR G  PNYRTLG GFQ
Sbjct: 61  HTESASLLFDEITLKDVVAWTALIVGHVQNNESEKGLKCLFDMHRYGGAPNYRTLGSGFQ 120

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 121 ACVDLDALVEGRCLHGLALKTGILCFEVIKSCLLSMYSRCGSPEEAYRCFCKLNKKDLIS 180

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          R SEGKAFHGWI 
Sbjct: 181 WTSIIAVHAKFGLMTECLSLFWEMQADEIIPDEIAISCMVLGFGNSDRTSEGKAFHGWIL 240

BLAST of Lag0013310 vs. NCBI nr
Match: XP_022154319.1 (pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 [Momordica charantia] >XP_022154320.1 pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 [Momordica charantia] >XP_022154321.1 pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 332.8 bits (852), Expect = 2.3e-87
Identity = 185/338 (54.73%), Postives = 201/338 (59.47%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFTVP+V+STCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 119 MRASSSLPNQFTVPMVLSTCAELMMLNHGMNIHGLALKLGLFYGNSAVGSSLVYMYSKCG 178

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
             ESASLLFDEIT+KDVVAWTALIVGHVQ+NESEKGLKCL++MHR G  PNYRTLG GFQ
Sbjct: 179 HTESASLLFDEITLKDVVAWTALIVGHVQNNESEKGLKCLFDMHRYGGAPNYRTLGSGFQ 238

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 239 ACVDLDALVEGRCLHGLALKTGILCFEVIKSCLLSMYSRCGSPEEAYRCFCKLNKKDLIS 298

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          R SEGKAFHGWI 
Sbjct: 299 WTSIIAVHAKFGLMTECLSLFWEMQADEIIPDEIAISCMVLGFGNSDRTSEGKAFHGWIL 358

BLAST of Lag0013310 vs. ExPASy Swiss-Prot
Match: Q3E9N1 (Pentatricopeptide repeat-containing protein At4g39952, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E98 PE=2 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 1.5e-41
Identity = 116/342 (33.92%), Postives = 152/342 (44.44%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLG-------------------L 60
           M  SG  P+ FT P+VVS CAEL+  + G  +HGL LK G                    
Sbjct: 116 MLLSGQSPDHFTAPMVVSACAELLWFHVGTFVHGLVLKHGGFDRNTAVGASFVYFYSKCG 175

Query: 61  FAESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNG---ETPNYRTLGG 120
           F + A L+FDE+  +DVVAWTA+I GHVQ+ ESE GL  L +MH  G   + PN RTL  
Sbjct: 176 FLQDACLVFDEMPDRDVVAWTAIISGHVQNGESEGGLGYLCKMHSAGSDVDKPNPRTLEC 235

Query: 121 GFQACVDMDALVEAR--------------------------------------------- 180
           GFQAC ++ AL E R                                             
Sbjct: 236 GFQACSNLGALKEGRCLHGFAVKNGLASSKFVQSSMFSFYSKSGNPSEAYLSFRELGDED 295

Query: 181 ---------------------------------------------------ISEGKAFHG 224
                                                              + +GKAFHG
Sbjct: 296 MFSWTSIIASLARSGDMEESFDMFWEMQNKGMHPDGVVISCLINELGKMMLVPQGKAFHG 355

BLAST of Lag0013310 vs. ExPASy Swiss-Prot
Match: Q9ZUW3 (Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H60 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 67/233 (28.76%), Postives = 122/233 (52.36%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTC---------AELMMLNH------GMSIHGLALKLGLFAES 60
           M+  G  PN+FT  ++++           A+++  N+      G ++    +KLG   E 
Sbjct: 388 MKRKGVRPNEFTYSVILTALPVISPSEVHAQVVKTNYERSSTVGTALLDAYVKLGK-VEE 447

Query: 61  ASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQACVD 120
           A+ +F  I  KD+VAW+A++ G+ Q+ E+E  +K   E+ + G  PN  T    F + ++
Sbjct: 448 AAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFT----FSSILN 507

Query: 121 MDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKSSKE 180
           + A   A + +GK FHG+  +     S     A+L+MY K G + +AE++F    +    
Sbjct: 508 VCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLV 567

Query: 181 DWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIG 219
            WN+M+ GY+  GQ  K +D F+EM+   ++ D ++ + V ++C+  G +  G
Sbjct: 568 SWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEG 615

BLAST of Lag0013310 vs. ExPASy Swiss-Prot
Match: O64705 (Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E23 PE=3 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 7.5e-25
Identity = 71/239 (29.71%), Postives = 114/239 (47.70%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLFAE---------------- 60
           M+ SG  P++FT   V   CA+L  +  G S+H    K+GL  +                
Sbjct: 123 MKFSGLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQ 182

Query: 61  --SASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQA 120
              A  LFDEIT +D V+W ++I G+ ++  ++  +    +M   G  P+ RTL     A
Sbjct: 183 VGYARKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGA 242

Query: 121 CVDMDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKS 180
           C  +  L   R+ E  A    I      GS      ++SMY K G L +A ++F+ + K 
Sbjct: 243 CSHLGDLRTGRLLEEMAITKKIGLSTFLGS-----KLISMYGKCGDLDSARRVFNQMIKK 302

Query: 181 SKEDWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSV 222
            +  W  M+  YS  G+  +    F EM+  G+ PD  +L +V+S+C  VGA+ +G+ +
Sbjct: 303 DRVAWTAMITVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQI 356

BLAST of Lag0013310 vs. ExPASy Swiss-Prot
Match: Q9ZQ74 (Pentatricopeptide repeat-containing protein At2g03380, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-E47 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.7e-24
Identity = 65/223 (29.15%), Postives = 113/223 (50.67%), Query Frame = 0

Query: 17  VSTCAELMMLNHGMSIHGLALKLGLF-----------------AESASLLFDEITVKDVV 76
           +  C EL  L++G  IH   +K+  F                  +SA  +F++IT+++VV
Sbjct: 149 LKACTELQDLDNGKKIHCQLVKVPSFDNVVLTGLLDMYAKCGEIKSAHKVFNDITLRNVV 208

Query: 77  AWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQACVDMDALVEARISEGKA 136
            WT++I G+V+++  E+GL     M  N    N  T G    AC  + AL      +GK 
Sbjct: 209 CWTSMIAGYVKNDLCEEGLVLFNRMRENNVLGNEYTYGTLIMACTKLSAL-----HQGKW 268

Query: 137 FHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKSSKEDWNTMMLGYSNTGQ 196
           FHG + +     S     ++L MY K G +S A ++F+         W  M++GY++ G 
Sbjct: 269 FHGCLVKSGIELSSCLVTSLLDMYVKCGDISNARRVFNEHSHVDLVMWTAMIVGYTHNGS 328

Query: 197 KEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSVH 223
             + +  F++M+ + I+P+ +++ SV+S C  +  + +GRSVH
Sbjct: 329 VNEALSLFQKMKGVEIKPNCVTIASVLSGCGLIENLELGRSVH 366

BLAST of Lag0013310 vs. ExPASy Swiss-Prot
Match: O04659 (Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E14 PE=2 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 3.7e-24
Identity = 65/240 (27.08%), Postives = 112/240 (46.67%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGL------------------F 60
           M +SG  PN  ++ + +S C+ L+ L  G  IH   +K G                    
Sbjct: 199 MESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDC 258

Query: 61  AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQA 120
            E A  +F ++  K +VAW ++I G+V   +S+  ++ L  M   G  P+  TL     A
Sbjct: 259 LEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTLTSILMA 318

Query: 121 CVDMDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKS 180
           C     L+      GK  HG++ R           +++ +Y K G  + AE +F    K 
Sbjct: 319 CSRSRNLL-----HGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQKD 378

Query: 181 SKEDWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSVH 223
             E WN M+  Y + G   K ++ + +M  +G++PD+++  SV+ +CSQ+ A+  G+ +H
Sbjct: 379 VAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKGKQIH 433

BLAST of Lag0013310 vs. ExPASy TrEMBL
Match: A0A6J1JGK6 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111484200 PE=4 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 6.5e-88
Identity = 185/338 (54.73%), Postives = 206/338 (60.95%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFT+P+VVSTCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 122 MRASSSLPNQFTIPMVVSTCAELMMLNHGMNIHGLALKLGLFVGNSAVGSSLIYMYSKCG 181

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
            AESASL+F++ITVKDVVAWTALI+G+VQ+NESEKGLKCL+EMHRNG TPNYRT+GGGFQ
Sbjct: 182 NAESASLMFNDITVKDVVAWTALIIGYVQNNESEKGLKCLFEMHRNGCTPNYRTIGGGFQ 241

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD++ALVE                                                  
Sbjct: 242 ACVDLEALVEGRCLHGLALKSGFLCFEVVKSSILSMYSRCGSPEEAYRCFFKLEQKDLIS 301

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          RISEGKAFH WI 
Sbjct: 302 WTSIMAVHSKLGLMSECLHLFWEMQASGIIPDDIVISCMLLGFGNFDRISEGKAFHAWIL 361

BLAST of Lag0013310 vs. ExPASy TrEMBL
Match: A0A6J1DLQ3 (pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021614 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.1e-87
Identity = 185/338 (54.73%), Postives = 201/338 (59.47%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFTVP+V+STCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 119 MRASSSLPNQFTVPMVLSTCAELMMLNHGMNIHGLALKLGLFYGNSAVGSSLVYMYSKCG 178

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
             ESASLLFDEIT+KDVVAWTALIVGHVQ+NESEKGLKCL++MHR G  PNYRTLG GFQ
Sbjct: 179 HTESASLLFDEITLKDVVAWTALIVGHVQNNESEKGLKCLFDMHRYGGAPNYRTLGSGFQ 238

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 239 ACVDLDALVEGRCLHGLALKTGILCFEVIKSCLLSMYSRCGSPEEAYRCFCKLNKKDLIS 298

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          R SEGKAFHGWI 
Sbjct: 299 WTSIIAVHAKFGLMTECLSLFWEMQADEIIPDEIAISCMVLGFGNSDRTSEGKAFHGWIL 358

BLAST of Lag0013310 vs. ExPASy TrEMBL
Match: A0A6J1DK10 (pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111021614 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.1e-87
Identity = 185/338 (54.73%), Postives = 201/338 (59.47%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFTVP+V+STCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 1   MRASSSLPNQFTVPMVLSTCAELMMLNHGMNIHGLALKLGLFYGNSAVGSSLVYMYSKCG 60

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
             ESASLLFDEIT+KDVVAWTALIVGHVQ+NESEKGLKCL++MHR G  PNYRTLG GFQ
Sbjct: 61  HTESASLLFDEITLKDVVAWTALIVGHVQNNESEKGLKCLFDMHRYGGAPNYRTLGSGFQ 120

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 121 ACVDLDALVEGRCLHGLALKTGILCFEVIKSCLLSMYSRCGSPEEAYRCFCKLNKKDLIS 180

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          R SEGKAFHGWI 
Sbjct: 181 WTSIIAVHAKFGLMTECLSLFWEMQADEIIPDEIAISCMVLGFGNSDRTSEGKAFHGWIL 240

BLAST of Lag0013310 vs. ExPASy TrEMBL
Match: A0A6J1FVR1 (LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448916 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.6e-86
Identity = 184/338 (54.44%), Postives = 203/338 (60.06%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFT+P+VVSTCAELMMLNHGM+IHGLALKLGLF                  
Sbjct: 122 MRASSSLPNQFTIPMVVSTCAELMMLNHGMNIHGLALKLGLFVGNSAVGSSLIYMYSKCG 181

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
            AESASL+F+EITVKDVVAWTALI+G+VQ+NESEKGLKCL+EMHRNG TPNYRT+GGGFQ
Sbjct: 182 NAESASLMFNEITVKDVVAWTALIIGYVQNNESEKGLKCLFEMHRNGCTPNYRTIGGGFQ 241

Query: 121 ACVDMDALVEA------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 242 ACVDLDALVEGRCLHGLALKSGFLCFEVVKSSILSMYSRCGSPEEAYRCFFKLEQKDLIS 301

Query: 181 -----------------------------------------------RISEGKAFHGWIP 224
                                                          RISEG AFH WI 
Sbjct: 302 WTSIIAVHSKLGLMSECLHLFWEMQASGIIPDDIVISCMLLGFGNFDRISEGNAFHAWIL 361

BLAST of Lag0013310 vs. ExPASy TrEMBL
Match: A0A1S3C9K9 (pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498381 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 5.0e-80
Identity = 175/338 (51.78%), Postives = 193/338 (57.10%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLF------------------ 60
           MRAS SLPNQFTVP+VVSTCAELMM NHGM+IHGL  KLGLF                  
Sbjct: 174 MRASSSLPNQFTVPMVVSTCAELMMFNHGMNIHGLTSKLGLFVSNSAIGSSFIYMYSKCG 233

Query: 61  -AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQ 120
             ESASL+F EITVKDVVAWTALIVG+VQ+NES +GLKCL+EMHR G TPNY+T+G GFQ
Sbjct: 234 HVESASLMFSEITVKDVVAWTALIVGYVQNNESGRGLKCLFEMHRIGGTPNYKTIGSGFQ 293

Query: 121 ACVDMDALVE-------------------------------------------------- 180
           ACVD+DALVE                                                  
Sbjct: 294 ACVDLDALVEGKCLHGLALKNGFLCFKVVKSTILSMYSRCGSPEEAYRCFCKLDQKDLIS 353

Query: 181 ----------------------------------------------ARISEGKAFHGWIP 224
                                                          RI EGKAFH WI 
Sbjct: 354 WTSIIAVHSKFGLMSECLHLFWEMQDSEIIPDEIVISCMLMGFGNSGRIFEGKAFHAWIL 413

BLAST of Lag0013310 vs. TAIR 10
Match: AT4G39952.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.1e-42
Identity = 116/342 (33.92%), Postives = 152/342 (44.44%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLG-------------------L 60
           M  SG  P+ FT P+VVS CAEL+  + G  +HGL LK G                    
Sbjct: 116 MLLSGQSPDHFTAPMVVSACAELLWFHVGTFVHGLVLKHGGFDRNTAVGASFVYFYSKCG 175

Query: 61  FAESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNG---ETPNYRTLGG 120
           F + A L+FDE+  +DVVAWTA+I GHVQ+ ESE GL  L +MH  G   + PN RTL  
Sbjct: 176 FLQDACLVFDEMPDRDVVAWTAIISGHVQNGESEGGLGYLCKMHSAGSDVDKPNPRTLEC 235

Query: 121 GFQACVDMDALVEAR--------------------------------------------- 180
           GFQAC ++ AL E R                                             
Sbjct: 236 GFQACSNLGALKEGRCLHGFAVKNGLASSKFVQSSMFSFYSKSGNPSEAYLSFRELGDED 295

Query: 181 ---------------------------------------------------ISEGKAFHG 224
                                                              + +GKAFHG
Sbjct: 296 MFSWTSIIASLARSGDMEESFDMFWEMQNKGMHPDGVVISCLINELGKMMLVPQGKAFHG 355

BLAST of Lag0013310 vs. TAIR 10
Match: AT2G27610.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.1e-26
Identity = 67/233 (28.76%), Postives = 122/233 (52.36%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTC---------AELMMLNH------GMSIHGLALKLGLFAES 60
           M+  G  PN+FT  ++++           A+++  N+      G ++    +KLG   E 
Sbjct: 388 MKRKGVRPNEFTYSVILTALPVISPSEVHAQVVKTNYERSSTVGTALLDAYVKLGK-VEE 447

Query: 61  ASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQACVD 120
           A+ +F  I  KD+VAW+A++ G+ Q+ E+E  +K   E+ + G  PN  T    F + ++
Sbjct: 448 AAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFT----FSSILN 507

Query: 121 MDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKSSKE 180
           + A   A + +GK FHG+  +     S     A+L+MY K G + +AE++F    +    
Sbjct: 508 VCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKRQREKDLV 567

Query: 181 DWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIG 219
            WN+M+ GY+  GQ  K +D F+EM+   ++ D ++ + V ++C+  G +  G
Sbjct: 568 SWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVTFIGVFAACTHAGLVEEG 615

BLAST of Lag0013310 vs. TAIR 10
Match: AT2G34400.1 (Pentatricopeptide repeat (PPR-like) superfamily protein )

HSP 1 Score: 115.5 bits (288), Expect = 5.3e-26
Identity = 71/239 (29.71%), Postives = 114/239 (47.70%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGLFAE---------------- 60
           M+ SG  P++FT   V   CA+L  +  G S+H    K+GL  +                
Sbjct: 123 MKFSGLKPDKFTYNFVFIACAKLEEIGVGRSVHSSLFKVGLERDVHINHSLIMMYAKCGQ 182

Query: 61  --SASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQA 120
              A  LFDEIT +D V+W ++I G+ ++  ++  +    +M   G  P+ RTL     A
Sbjct: 183 VGYARKLFDEITERDTVSWNSMISGYSEAGYAKDAMDLFRKMEEEGFEPDERTLVSMLGA 242

Query: 121 CVDMDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKS 180
           C  +  L   R+ E  A    I      GS      ++SMY K G L +A ++F+ + K 
Sbjct: 243 CSHLGDLRTGRLLEEMAITKKIGLSTFLGS-----KLISMYGKCGDLDSARRVFNQMIKK 302

Query: 181 SKEDWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSV 222
            +  W  M+  YS  G+  +    F EM+  G+ PD  +L +V+S+C  VGA+ +G+ +
Sbjct: 303 DRVAWTAMITVYSQNGKSSEAFKLFFEMEKTGVSPDAGTLSTVLSACGSVGALELGKQI 356

BLAST of Lag0013310 vs. TAIR 10
Match: AT2G03380.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.6e-25
Identity = 65/223 (29.15%), Postives = 113/223 (50.67%), Query Frame = 0

Query: 17  VSTCAELMMLNHGMSIHGLALKLGLF-----------------AESASLLFDEITVKDVV 76
           +  C EL  L++G  IH   +K+  F                  +SA  +F++IT+++VV
Sbjct: 149 LKACTELQDLDNGKKIHCQLVKVPSFDNVVLTGLLDMYAKCGEIKSAHKVFNDITLRNVV 208

Query: 77  AWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQACVDMDALVEARISEGKA 136
            WT++I G+V+++  E+GL     M  N    N  T G    AC  + AL      +GK 
Sbjct: 209 CWTSMIAGYVKNDLCEEGLVLFNRMRENNVLGNEYTYGTLIMACTKLSAL-----HQGKW 268

Query: 137 FHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKSSKEDWNTMMLGYSNTGQ 196
           FHG + +     S     ++L MY K G +S A ++F+         W  M++GY++ G 
Sbjct: 269 FHGCLVKSGIELSSCLVTSLLDMYVKCGDISNARRVFNEHSHVDLVMWTAMIVGYTHNGS 328

Query: 197 KEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSVH 223
             + +  F++M+ + I+P+ +++ SV+S C  +  + +GRSVH
Sbjct: 329 VNEALSLFQKMKGVEIKPNCVTIASVLSGCGLIENLELGRSVH 366

BLAST of Lag0013310 vs. TAIR 10
Match: AT5G27110.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.6e-25
Identity = 65/240 (27.08%), Postives = 112/240 (46.67%), Query Frame = 0

Query: 1   MRASGSLPNQFTVPLVVSTCAELMMLNHGMSIHGLALKLGL------------------F 60
           M +SG  PN  ++ + +S C+ L+ L  G  IH   +K G                    
Sbjct: 199 MESSGFEPNSVSLTVAISACSRLLWLERGKEIHRKCVKKGFELDEYVNSALVDMYGKCDC 258

Query: 61  AESASLLFDEITVKDVVAWTALIVGHVQSNESEKGLKCLYEMHRNGETPNYRTLGGGFQA 120
            E A  +F ++  K +VAW ++I G+V   +S+  ++ L  M   G  P+  TL     A
Sbjct: 259 LEVAREVFQKMPRKSLVAWNSMIKGYVAKGDSKSCVEILNRMIIEGTRPSQTTLTSILMA 318

Query: 121 CVDMDALVEARISEGKAFHGWIPRQCCAGSGVAQDAILSMYCKLGLLSTAEKIFHSLHKS 180
           C     L+      GK  HG++ R           +++ +Y K G  + AE +F    K 
Sbjct: 319 CSRSRNLL-----HGKFIHGYVIRSVVNADIYVNCSLIDLYFKCGEANLAETVFSKTQKD 378

Query: 181 SKEDWNTMMLGYSNTGQKEKCIDFFREMQLLGIEPDLISLVSVISSCSQVGAINIGRSVH 223
             E WN M+  Y + G   K ++ + +M  +G++PD+++  SV+ +CSQ+ A+  G+ +H
Sbjct: 379 VAESWNVMISSYISVGNWFKAVEVYDQMVSVGVKPDVVTFTSVLPACSQLAALEKGKQIH 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010985.13.5e-8855.03Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
KAG6571177.13.5e-8855.03Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022986468.11.3e-8754.73LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mito... [more]
XP_022154323.12.3e-8754.73pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X3 ... [more]
XP_022154319.12.3e-8754.73pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 ... [more]
Match NameE-valueIdentityDescription
Q3E9N11.5e-4133.92Pentatricopeptide repeat-containing protein At4g39952, mitochondrial OS=Arabidop... [more]
Q9ZUW31.5e-2528.76Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana OX... [more]
O647057.5e-2529.71Pentatricopeptide repeat-containing protein At2g34400 OS=Arabidopsis thaliana OX... [more]
Q9ZQ743.7e-2429.15Pentatricopeptide repeat-containing protein At2g03380, mitochondrial OS=Arabidop... [more]
O046593.7e-2427.08Pentatricopeptide repeat-containing protein At5g27110 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1JGK66.5e-8854.73LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mito... [more]
A0A6J1DLQ31.1e-8754.73pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 ... [more]
A0A6J1DK101.1e-8754.73pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X3 ... [more]
A0A6J1FVR14.6e-8654.44LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein At4g39952, mito... [more]
A0A1S3C9K95.0e-8051.78pentatricopeptide repeat-containing protein At4g39952, mitochondrial isoform X1 ... [more]
Match NameE-valueIdentityDescription
AT4G39952.11.1e-4233.92Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G27610.11.1e-2628.76Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G34400.15.3e-2629.71Pentatricopeptide repeat (PPR-like) superfamily protein [more]
AT2G03380.12.6e-2529.15Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G27110.12.6e-2527.08Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 59..92
e-value: 2.1E-5
score: 22.4
coord: 167..198
e-value: 1.0E-6
score: 26.5
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 59..88
e-value: 4.2E-4
score: 20.4
coord: 138..161
e-value: 0.04
score: 14.2
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 167..209
e-value: 3.2E-7
score: 30.4
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 57..91
score: 9.503505
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 163..197
score: 10.533867
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..111
e-value: 3.4E-7
score: 32.0
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 112..217
e-value: 2.6E-15
score: 58.2
NoneNo IPR availablePANTHERPTHR47929FAMILY NOT NAMEDcoord: 1..222

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0013310.1Lag0013310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding