Lag0013121 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0013121 vs. NCBI nr
Match: KAA0039950.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24553.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-61
Identity = 123/362 (33.98%), Postives = 193/362 (53.31%), Query Frame = 0

Query: 1    MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
            +P Y +S+FK+P  +A+ ++  +R+F W G+ +   +  I W  +  P   GG+GI +  
Sbjct: 731  LPIYQMSVFKVPKGIAQKIEASWRNFLWNGASNGHNISLIRWNQIVSPKEKGGLGIHSVN 790

Query: 61   NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
            + N ALL KW+W+FL E++ LW RLI++KY   ++ S +PS  + SS+ SPW+ +T  I 
Sbjct: 791  STNFALLCKWLWKFLTEKDPLWKRLIISKYDKEKMGS-FPSHGKFSSNNSPWKAVTECIS 850

Query: 121  LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                 +  ++ +G D SFW D+W     L+   PRL+ L+   +  V E W  S   W  
Sbjct: 851  WFYKNISWKVNDGEDISFWLDNWNGNAPLSLAVPRLFALSTNKKGSVKEFWNPSSNDWHL 910

Query: 181  SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDYP---- 240
             + R L D E   W  +   L +          +W L S+NIF   S+   + + P    
Sbjct: 911  HINRPLRDHEENLWHNIKASLPTPLPNRGHPKPLWNLNSNNIFDTASVKRAIAEAPISPA 970

Query: 241  NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
            N   +LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 971  NFHPNLYKTLWKVEFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 1030

Query: 301  SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVLTLIFVDHPFRGEKKILWLALNRVF 359
              HLF+HC ++ + WS+      W++  P  ++ ++  I      R +K ++    N   
Sbjct: 1031 INHLFIHCPYSQQLWSKAKALLNWNST-PTDVQSLIQNI-CSLNIRNQKGLITFNTNATI 1089

BLAST of Lag0013121 vs. NCBI nr
Match: KAA0041397.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-61
Identity = 122/335 (36.42%), Postives = 185/335 (55.22%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P Y LS+FK+P  +A+ ++  +R+F W G+ +   +  I W  V  P   GG+GI +  
Sbjct: 623 LPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHSVH 682

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
           + N ALL KW+W+FL E+  LW RLI++K Y+ E    +PS  + SS+ SPW+ +T+ I 
Sbjct: 683 STNFALLCKWLWKFLTEKEPLWKRLIISK-YDQEKMGRFPSRGKYSSNNSPWKAVTNCIS 742

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                +  ++ +G D SFW D+W     L+   PRL+ L+   +  V + W  S   W+ 
Sbjct: 743 WFYKNIGWKVNDGEDISFWLDNWNGNSPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNI 802

Query: 181 SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDY----P 240
            + R L D E   W  +   L +          +W L S+NIF   S+ +DL +      
Sbjct: 803 HVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPT 862

Query: 241 NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
           N    LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 863 NFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 922

Query: 301 SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDV 332
             HLF+HC ++ + WS+      W++  PN +K +
Sbjct: 923 INHLFIHCPYSQQLWSKAQALLKWNST-PNDVKSL 955

BLAST of Lag0013121 vs. NCBI nr
Match: KAA0044556.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-61
Identity = 122/335 (36.42%), Postives = 184/335 (54.93%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P Y LS+FK+P  +A+ ++  +R+F W G+ +   +  I W  V  P   GG+GI    
Sbjct: 529 LPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHFVH 588

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
           + N ALL KW+W+FL E+  LW RLI++K Y+ E    +PS  + SS+ SPW+ +T+ I 
Sbjct: 589 STNFALLCKWLWKFLTEKEPLWKRLIISK-YDQEKMGRFPSRGKYSSNNSPWKAVTNCIS 648

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                +  ++ +G D SFW D+W     L+   PRL+ L+   +  V + W  S   W+ 
Sbjct: 649 WFYKNIGWKVNDGEDISFWLDNWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLKDWNI 708

Query: 181 SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDY----P 240
            + R L D E   W  +   L +          +W L S+NIF   S+ +DL +      
Sbjct: 709 HVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPT 768

Query: 241 NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
           N    LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 769 NFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 828

Query: 301 SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDV 332
             HLF+HC ++ + WS+      W++  PN +K +
Sbjct: 829 INHLFIHCPYSQQLWSKAQALLKWNST-PNDVKSL 861

BLAST of Lag0013121 vs. NCBI nr
Match: BBN69274.1 (TatD related DNase [Prunus dulcis])

HSP 1 Score: 238.8 bits (608), Expect = 8.0e-59
Identity = 135/366 (36.89%), Postives = 190/366 (51.91%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P+YY+SLFK+P  V   +++L R+F WEG       H + W  V      GG+GIG+ +
Sbjct: 559 IPSYYMSLFKMPIGVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGSLR 618

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
            RN AL AKW+WRF  E NSLW+R+I +K Y  +        I K S ++PWR I+    
Sbjct: 619 ERNEALRAKWLWRFPLETNSLWHRIIKSK-YGLDSNGWDTKQIDKVSCRNPWREISKGYI 678

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQ----T 180
                 +  +GNG    FW D WL  GIL   FPRLY L+ R    +   W A+      
Sbjct: 679 SFLQCCRFSVGNGEKIRFWEDFWLKEGILKDLFPRLYSLSRRKNQCI--AWFANNHEMPL 738

Query: 181 TWDPSLRRNLNDVETEEWMALSLILSSISL-QNCTDSWIWPLESSNIFSVKSLMEDLVDY 240
            WD   RRNL++ E  E + L  IL ++ L  +  D   W +E    FS KS    L+  
Sbjct: 739 NWDFDFRRNLSEAEIAEVVLLLDILGNVRLFGSRPDRRSWEIEEQGSFSCKSFRSFLIST 798

Query: 241 PNMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSE 300
            ++    Y+ IW    P KI+ F+W  ++G INT D +QRR P   LSPSWC+ C  ++E
Sbjct: 799 TSVVFPPYRSIWKAKTPPKIQFFVWLAANGRINTCDCIQRRQPKMCLSPSWCVFCKENAE 858

Query: 301 HSRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVLTLIFVDHPFRGEKK---ILWLAL 359
           +  HLF+HC+++ + W  +LDA G   V P   K    L+ ++    G+ K   IL   L
Sbjct: 859 NIDHLFIHCSYSLKLWWRMLDALGAEWVIP---KGCFELLSINLRISGKGKKAGILRDCL 918

BLAST of Lag0013121 vs. NCBI nr
Match: TQD93576.1 (hypothetical protein C1H46_020784 [Malus baccata])

HSP 1 Score: 233.4 bits (594), Expect = 3.3e-57
Identity = 130/364 (35.71%), Postives = 188/364 (51.65%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P YY+SLFK+P  V   L+KL + F WEG       H + W  V      GG+G+GN +
Sbjct: 95  LPIYYMSLFKIPCGVIGRLEKLMKGFLWEGVEEGKKNHLVKWEIVIKSKEEGGLGVGNLR 154

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKY--YNSELPSLWPSIIQKSSHKSPWRFITST 120
           NRN ALLAKW+WRF  E +SLW+++I +KY   N+   +  P    + S +SPW+ I+S 
Sbjct: 155 NRNEALLAKWLWRFPKEPHSLWHKVIRSKYGLQNNGWNAFPP---MRGSSRSPWKDISSG 214

Query: 121 IDLVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGE--TWIASQT 180
             L     K  +GNG    FW D WL  G L   FPRL+ L+      +     +  +  
Sbjct: 215 SQLFLHCCKFEVGNGERVRFWEDGWLEGGPLKEQFPRLFLLSRMHNQNISSFADFSTNSL 274

Query: 181 TWDPSLRRNLNDVETEEWMALSLILSSISL-QNCTDSWIWPLESSNIFSVKSLMEDLVDY 240
           +W+   RRNLN+ E EE   L   +  + L Q+ TD+  W LE+S +F+ KS    L + 
Sbjct: 275 SWNFDFRRNLNEAEIEEAARLLQKVEEVRLSQSRTDNRRWKLEASRLFTCKSYRSFLSNN 334

Query: 241 PNMAN-DLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASS 300
             M     Y  IW    P K+KI +W ++ G +NT D++QRR     LSP WC +C A  
Sbjct: 335 GIMQYFPPYSQIWKSKVPPKVKILVWLVAKGKLNTCDQIQRRSHFICLSPHWCSLCKAKE 394

Query: 301 EHSRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVLTLIFVDHPFRGEKKILWLALNR 359
           E   H+F+HC++  + W ++      S V P    ++L+  F       + K+LW  L  
Sbjct: 395 ESVNHIFLHCSYTIQLWWKLFQEVRASWVIPKGCFELLSTKFQALGIGRKAKVLWGCLVS 454

BLAST of Lag0013121 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.5e-28
Identity = 99/323 (30.65%), Postives = 143/323 (44.27%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           MP + +S   LP  +   LD+L R F W  +      H + W+ V  P   GG+G+   +
Sbjct: 48  MPVHSMSTILLPQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAK 107

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSEL-PSLWPSIIQKSSHKSPWRFITSTI 120
           + N AL++K  WR L E+NSLW  ++  KY+  E+  S W  +I K S  S WR I   +
Sbjct: 108 SMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGEIRDSRW--LIPKGSWSSTWRSIAIGL 167

Query: 121 -DLVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTW 180
            D+VS  V    G+G    FW D W+S G          R TD    +  + WI  +  W
Sbjct: 168 RDVVSHGVGWIPGDGQQIRFWTDRWVS-GKPLLELDNGERPTDCDTVVAKDLWIPGR-GW 227

Query: 181 DPSLRRNLNDVETE-EWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLME----DLV 240
           D +         T  E  A+ L L    +    D   W       FSV+S  E    D V
Sbjct: 228 DFAKIDPYTTNNTRLELRAVVLDL----VTGARDRLSWKFSQDGQFSVRSAYEMLTVDEV 287

Query: 241 DYPNMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPS-WCIMCAA 300
             PNMA+  +  +W    P+++K FLW + + A+ T +   RR    HLS S  C +C  
Sbjct: 288 PRPNMAS-FFNCLWKVRVPERVKTFLWLVGNQAVMTEEERHRR----HLSASNVCQVCKG 347

Query: 301 SSEHSRHLFVHCTFASRYWSEIL 316
             E   H+   C      W  ++
Sbjct: 348 GVESMLHVLRDCPAQLGIWVRVV 357

BLAST of Lag0013121 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.6e-09
Identity = 40/148 (27.03%), Postives = 72/148 (48.65%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATV-QLPHLMGGIGIGNF 60
           +P Y +S F+L   + K L     +F+W    +   +  + W  + +     GG+G  + 
Sbjct: 3   LPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDL 62

Query: 61  QNRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSP---WRFIT 120
              N ALLAK  +R +H+ ++L  RL+ ++Y+         S+++ S    P   WR I 
Sbjct: 63  GWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHS------SMMECSVGTRPSYAWRSII 122

Query: 121 STIDLVSSRVKRRLGNGLDTSFWHDSWL 145
              +L+S  + R +G+G+ T  W D W+
Sbjct: 123 HGRELLSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lag0013121 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 4.9e-62
Identity = 123/362 (33.98%), Postives = 193/362 (53.31%), Query Frame = 0

Query: 1    MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
            +P Y +S+FK+P  +A+ ++  +R+F W G+ +   +  I W  +  P   GG+GI +  
Sbjct: 731  LPIYQMSVFKVPKGIAQKIEASWRNFLWNGASNGHNISLIRWNQIVSPKEKGGLGIHSVN 790

Query: 61   NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
            + N ALL KW+W+FL E++ LW RLI++KY   ++ S +PS  + SS+ SPW+ +T  I 
Sbjct: 791  STNFALLCKWLWKFLTEKDPLWKRLIISKYDKEKMGS-FPSHGKFSSNNSPWKAVTECIS 850

Query: 121  LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                 +  ++ +G D SFW D+W     L+   PRL+ L+   +  V E W  S   W  
Sbjct: 851  WFYKNISWKVNDGEDISFWLDNWNGNAPLSLAVPRLFALSTNKKGSVKEFWNPSSNDWHL 910

Query: 181  SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDYP---- 240
             + R L D E   W  +   L +          +W L S+NIF   S+   + + P    
Sbjct: 911  HINRPLRDHEENLWHNIKASLPTPLPNRGHPKPLWNLNSNNIFDTASVKRAIAEAPISPA 970

Query: 241  NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
            N   +LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 971  NFHPNLYKTLWKVEFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 1030

Query: 301  SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVLTLIFVDHPFRGEKKILWLALNRVF 359
              HLF+HC ++ + WS+      W++  P  ++ ++  I      R +K ++    N   
Sbjct: 1031 INHLFIHCPYSQQLWSKAKALLNWNST-PTDVQSLIQNI-CSLNIRNQKGLITFNTNATI 1089

BLAST of Lag0013121 vs. ExPASy TrEMBL
Match: A0A5A7TIB8 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00430 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 6.4e-62
Identity = 122/335 (36.42%), Postives = 185/335 (55.22%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P Y LS+FK+P  +A+ ++  +R+F W G+ +   +  I W  V  P   GG+GI +  
Sbjct: 623 LPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHSVH 682

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
           + N ALL KW+W+FL E+  LW RLI++K Y+ E    +PS  + SS+ SPW+ +T+ I 
Sbjct: 683 STNFALLCKWLWKFLTEKEPLWKRLIISK-YDQEKMGRFPSRGKYSSNNSPWKAVTNCIS 742

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                +  ++ +G D SFW D+W     L+   PRL+ L+   +  V + W  S   W+ 
Sbjct: 743 WFYKNIGWKVNDGEDISFWLDNWNGNSPLSLVVPRLFALSTNKKGSVKDLWNPSLKDWNI 802

Query: 181 SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDY----P 240
            + R L D E   W  +   L +          +W L S+NIF   S+ +DL +      
Sbjct: 803 HVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPT 862

Query: 241 NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
           N    LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 863 NFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 922

Query: 301 SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDV 332
             HLF+HC ++ + WS+      W++  PN +K +
Sbjct: 923 INHLFIHCPYSQQLWSKAQALLKWNST-PNDVKSL 955

BLAST of Lag0013121 vs. ExPASy TrEMBL
Match: A0A5A7TR15 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003050 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.4e-61
Identity = 122/335 (36.42%), Postives = 184/335 (54.93%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P Y LS+FK+P  +A+ ++  +R+F W G+ +   +  I W  V  P   GG+GI    
Sbjct: 529 LPIYQLSVFKVPKGIAQKIEAYWRNFLWNGTSNGHNISLIRWNQVVSPKEKGGLGIHFVH 588

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
           + N ALL KW+W+FL E+  LW RLI++K Y+ E    +PS  + SS+ SPW+ +T+ I 
Sbjct: 589 STNFALLCKWLWKFLTEKEPLWKRLIISK-YDQEKMGRFPSRGKYSSNNSPWKAVTNCIS 648

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
                +  ++ +G D SFW D+W     L+   PRL+ L+   +  V + W  S   W+ 
Sbjct: 649 WFYKNIGWKVNDGEDISFWLDNWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLKDWNI 708

Query: 181 SLRRNLNDVETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVDY----P 240
            + R L D E   W  +   L +          +W L S+NIF   S+ +DL +      
Sbjct: 709 HVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEASASPT 768

Query: 241 NMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSEH 300
           N    LYK +W   +PKK K F+W L HG INTADRLQ+R+P++ LSP+WC MC  S E 
Sbjct: 769 NFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPNWTLSPNWCYMCNKSQED 828

Query: 301 SRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDV 332
             HLF+HC ++ + WS+      W++  PN +K +
Sbjct: 829 INHLFIHCPYSQQLWSKAQALLKWNST-PNDVKSL 861

BLAST of Lag0013121 vs. ExPASy TrEMBL
Match: A0A5H2XQW2 (TatD related DNase OS=Prunus dulcis OX=3755 GN=Prudu_862S001100 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 3.9e-59
Identity = 135/366 (36.89%), Postives = 190/366 (51.91%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +P+YY+SLFK+P  V   +++L R+F WEG       H + W  V      GG+GIG+ +
Sbjct: 559 IPSYYMSLFKMPIGVTAKVEQLMRNFLWEGVEEGKNCHLVRWVRVTKSKEEGGLGIGSLR 618

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
            RN AL AKW+WRF  E NSLW+R+I +K Y  +        I K S ++PWR I+    
Sbjct: 619 ERNEALRAKWLWRFPLETNSLWHRIIKSK-YGLDSNGWDTKQIDKVSCRNPWREISKGYI 678

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQ----T 180
                 +  +GNG    FW D WL  GIL   FPRLY L+ R    +   W A+      
Sbjct: 679 SFLQCCRFSVGNGEKIRFWEDFWLKEGILKDLFPRLYSLSRRKNQCI--AWFANNHEMPL 738

Query: 181 TWDPSLRRNLNDVETEEWMALSLILSSISL-QNCTDSWIWPLESSNIFSVKSLMEDLVDY 240
            WD   RRNL++ E  E + L  IL ++ L  +  D   W +E    FS KS    L+  
Sbjct: 739 NWDFDFRRNLSEAEIAEVVLLLDILGNVRLFGSRPDRRSWEIEEQGSFSCKSFRSFLIST 798

Query: 241 PNMANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCIMCAASSE 300
            ++    Y+ IW    P KI+ F+W  ++G INT D +QRR P   LSPSWC+ C  ++E
Sbjct: 799 TSVVFPPYRSIWKAKTPPKIQFFVWLAANGRINTCDCIQRRQPKMCLSPSWCVFCKENAE 858

Query: 301 HSRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVLTLIFVDHPFRGEKK---ILWLAL 359
           +  HLF+HC+++ + W  +LDA G   V P   K    L+ ++    G+ K   IL   L
Sbjct: 859 NIDHLFIHCSYSLKLWWRMLDALGAEWVIP---KGCFELLSINLRISGKGKKAGILRDCL 918

BLAST of Lag0013121 vs. ExPASy TrEMBL
Match: A0A803P465 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 2.5e-58
Identity = 126/344 (36.63%), Postives = 183/344 (53.20%), Query Frame = 0

Query: 1    MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
            +P Y+LSLFK P  V K L+K+ RDF WEGS S GG H + W  V  P   GG+GIG  +
Sbjct: 1504 LPLYFLSLFKAPRSVTKALEKMMRDFLWEGSESSGGEHLVAWDEVCRPRHEGGLGIGRLE 1563

Query: 61   NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSII-QKSSHKSPWRFITSTI 120
             RN +LL KW+WRF  E+NSLW+R++V++Y  ++  +LW S    + S K PWR I+   
Sbjct: 1564 LRNKSLLMKWLWRFPLEQNSLWHRVVVSRYGRAD--NLWDSKKGSRLSPKGPWRDISDLY 1623

Query: 121  DLVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGET--------- 180
            D     V  +LG G    FW D W+    L + FP L  +++   + + E          
Sbjct: 1624 DEFLGLVHFKLGRGDRIRFWEDVWIDDYPLVSAFPDLAVISEGRNTPIKELIVDEGLPGG 1683

Query: 181  WIASQTTWDPSLRRNLNDVETEEWMALSLILSSISLQNCT-DSWIWPLESSNIFSVKSLM 240
            W+ S   W+   RRNL D E    ++L   +  + + + + DS IW  + S +FS KS  
Sbjct: 1684 WVES---WNFKFRRNLMDRELPSLISLMQKVEHVRVLSISEDSRIWKPDPSGVFSCKSAF 1743

Query: 241  EDLVDYPNM-ANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLSPSWCI 300
              +V  P+    D  K +W    P K+K+F W L+   +N  D++Q+R P  ++SP WC+
Sbjct: 1744 SWMVSNPSFRVLDWTKSLWKSCAPSKVKVFGWLLALEKVNVHDKVQKRKPFLYISPGWCV 1803

Query: 301  MCAASSEHSRHLFVHCTFASRYWSEILDAFGWSTVFPNCIKDVL 333
             C  S E   HLF+ C F  R W  +L  FG S V P  +  ++
Sbjct: 1804 CCKGSGESVGHLFLECKFIGRIWRYVLGEFGLSWVMPRSVSHLI 1842

BLAST of Lag0013121 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-24
Identity = 93/330 (28.18%), Postives = 136/330 (41.21%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATVQLPHLMGGIGIGNFQ 60
           +PTY ++ F LP  V K +  +  DF+W   +   GMH   W  +      GGIG  + +
Sbjct: 3   LPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIE 62

Query: 61  NRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSPWRFITSTID 120
             NLALL K +WR L    SL  ++  ++Y++   P   P     S     W+ I ++ +
Sbjct: 63  AFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAP---LGSRPSFVWKSIHASQE 122

Query: 121 LVSSRVKRRLGNGLDTSFWHDSWLSCGILATNFPRLYRLTDRPRSLVGETWIASQTTWDP 180
           ++    +  +GNG D   W   WL     A+   R+ R+  +  + V      S    D 
Sbjct: 123 ILRQGARAVVGNGEDIIIWRHKWLD-SKPASAALRMQRVPPQEYASVSSILKVSDLI-DE 182

Query: 181 SLRRNLNDV------ETEEWMALSLILSSISLQNCTDSWIWPLESSNIFSVKSLMEDLVD 240
           S R    DV      E E  +   L       +   DS+ W   SS  ++VKS    L  
Sbjct: 183 SGREWRKDVIEMLFPEVERKLIGELRPGG---RRILDSYTWDYTSSGDYTVKSGYWVLTQ 242

Query: 241 YPN-----------MANDLYKVIWTDFYPKKIKIFLWELSHGAINTADRLQRRMPHFHLS 300
             N             N +Y+ IW      KI+ FLW+    ++  A  L  R    HLS
Sbjct: 243 IINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYR----HLS 302

Query: 301 -PSWCIMCAASSEHSRHLFVHCTFASRYWS 313
             S CI C +  E   HL   CTFA   W+
Sbjct: 303 KESACIRCPSCKETVNHLLFKCTFARLTWA 320

BLAST of Lag0013121 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 63.9 bits (154), Expect = 3.3e-10
Identity = 40/148 (27.03%), Postives = 72/148 (48.65%), Query Frame = 0

Query: 1   MPTYYLSLFKLPGKVAKTLDKLFRDFFWEGSRSDGGMHNINWATV-QLPHLMGGIGIGNF 60
           +P Y +S F+L   + K L     +F+W    +   +  + W  + +     GG+G  + 
Sbjct: 3   LPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDL 62

Query: 61  QNRNLALLAKWIWRFLHEENSLWYRLIVAKYYNSELPSLWPSIIQKSSHKSP---WRFIT 120
              N ALLAK  +R +H+ ++L  RL+ ++Y+         S+++ S    P   WR I 
Sbjct: 63  GWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHS------SMMECSVGTRPSYAWRSII 122

Query: 121 STIDLVSSRVKRRLGNGLDTSFWHDSWL 145
              +L+S  + R +G+G+ T  W D W+
Sbjct: 123 HGRELLSRGLLRTIGDGIHTKVWLDRWI 144