Lag0013107 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0013107
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr1: 47508697 .. 47512449 (-)
RNA-Seq Expression	Lag0013107
Synteny	Lag0013107

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTAGACACTTACTTCCTCTACTCTTCCCTCTCGTTTCTCTTTCTTCTTTTCGCTTTCAACCTCATCCGTCGATCCCTTCGATTTCGCAACAACTTACCGCCGAGTCCGCCATCTCTTCCGGTCATCGGCCATCTCCATCTCTTGAAAAAGCCACTCCACCGGAATTTCCAGAACCTCTCCGCCAAATATGGCCCTGTTATGTCTCTCCGCCTCGGATCTCGCCTTGCAGTCGTTGTATCCTCTTCGTCGGCGGTGGAGGACTGCTTCACTAAGAACGACGTCGTTCTCGCCAACCGTCCTCGCTTGCTAATCGGCAAATACCTCGGCTACAACTACACTACCATGATTGGGGCTGCGTACGGCGACCACTGGCGGAACCTCCGCCGAATCGGCTCAATCGAAATCTTCTCTTTGTCTCGAATCAACAAATTCGCCGAAATTCGGAGGGACGAAGTGGAGCGATTGGTGCGGAAACTTTCGAGCAATTCGATCCATCAATTCTCGAAAGTAGAAATGCAATCGGCGTTGTTGGAGCTGACGTTCAACATCTCGATGAGAATGGTGGCCGGAAAGAGGTATTACGGCGACGAGGTGACGGACGAGGAAGAGGCGAGGCAGTTCAGGGAGTTGATTAAGCAGATTGTGGCGCTGGGAGGAGTATCAAATCCAGGGGATTTTTTGCCCATTCTGAACTGGATTTCGAAGGGTTTCGAGAGGAAATTGATTCAATTTGGGAAGAAGATGGATGCGTTCTTGCAGGGGCTGATCGACGACCACCTGAGGGAGAAGGAAGAGGGAAGGAACACGATGATCGATCACCTGCTCTCTCTGCAAGAATCGGATCCTGCTCAATACGACGACCAAATAATCAAAGGATTTATACTGGTAAACATAAACATATTTCTTCATTTCTCTAGCTATGCAGTTTCTCATCAATTTCATGTTTGCTTTAAAACTTTTTAAACATATCACCAAAATTAATTTTGAAATATCACGACCGTGATTTCGAATATGAAAAAAAAAAGAAAAAAAAAAGGTGATTTTAACACTTTCAAAATCATTTCCAAACATGCACTTAGTCGACTGAGCTCATTATGACTTAAAGTGATTTGGTTATCCATTCAACTTAGAATTACTCAAATTTTCAAAATTAAATTTAATATAACTTTTGTGATTGGAAATAATATCAACTTTATTAGTTTTAAAACATGGACAAAGTAAATTAAGGATAAGGTCTCAAAAAAAATTAAATTAAGGATAAGTAACAAGTTTAGTTGAGATTATTGTTCAGGAATTGGAAAATAAAGTTTAAATTCTTCATTGTCAGAAAAAAAAAAGTTTAAATTCTTCGTATAAATTTTAAACTAATTTAGTCCCTTTTTTCTTTAGATGAAAATGATTGAAAACGATTTAAGAAAATAAAATTACGAGTAAGTAAGATGTTATTTTCATTTTAGTACCCACAACTGTTTAGGGCAACTGTTGGGTTGAGAATCCCCCCCTTTTGAAATACTCCTTCGAACTATAGACCTTGTGATTATCAATACATGTGTTATCTTGATCATGGAATCATATTCGAGACTACATGTGTCAAAATTACTGCTATGTTTGCTTTGGCTAATTCACCTTATTTGTATTAAGTATATATTGTATCGATTGATTTGTAGGTGTTACTAACTGCTGGGACCGACACATCAGCTGTGACAATGGAATGGGCCTTGTCTCATCTGCTGAACAATCCTGAAGTGTTAAAGAAAGCAAAAGACGAACTAGACACTCAAATTGGACAAGAACGGCTCGTTGAAGAACCAGACATGTCCAAACTACCCTATCTCCAGGGAATCATCTCTGAGACTCTTCGATTGACTCCCGCCGTTCCAATGTTGCTGCCCCATTATGCAGCCAATGACTGCACAATAGGTGGATATAACGTGCCACGTGACACGATGGTACTAGTTAATGCATGGGCCATACATAGGGATCCAAAGGAATGGGAGAATCCCACAAGTTTCGTACCAGAACGATATGAAAATTCGTCAGACGAAGTTAAGGTACACAAGTTGATACCTTTCGGAGTAGGGAGGCGAGCTTGTCCTGGGTATGCTATGGCACAAAGAGTGATGGGTCTAACTTTGGCAACACTGGTTCAGTGCTTCGAGTGGGAGAGAGTTGGAGAAGAAAAAGTGGACATGCACGAGGGTGCTGGTGCCACAATGCCCAAGCTTCTGCCATTGGAGGCTATGTGCAAAGCTCGTCCCATCGTCAACAACCTTTTTGGTTGAGAAATAATTGTTACAAGTTTATAATTAACGTATGGCCTTTGTCTTATACTTACTCATTTTTCTTAGGCATTGTTGGGCTATATCATAGCATAATAATTAAAATTATTAATTTATTCTATGAGCATGTTTGTAGGCCTAATTTAGGATGGAAGTAGTCATGAATGTTGATGATCAAATTTCGCTAGGCAAATTACCTTCGCTCCACGTGATAATCGACAATGAATTTGTTTGAGAGCGGGGGAAAGACGTGACCTGCAAAACATAAACCTGCACATGTGGTGCTAGCCACACCGCCTCCGATGAAGTCGGAAAACGGAAGGGAGAGTTAGAGATAAATACCTGCTCGTGTTCCTAGGATTTTTAGGAATTCAGAGGCGTTTCAGGACAAACCAAGCGGAACCGGGTGGCCAGAGGCGGTAGGGACTAAACGGAGCCGGAGGGACTCGGCCTCGGCCATGCCCGACCCCTTAGGTCTTGGCCCGTCATGTTTGCCGGTTTCGTCTATAGGATCCATCTCCCAGCCTTATTTCTGTCTGGTTGTCATCGTCAGCTCCTTGTATACCGGAGTGGTCCAAAATTACCTATAACAATAAACTACGTTCAATCGTTCATCTGGACTAATTTACCTCTTATGTTAAAAATCCACTTGTGTTTAAGTATAGAATATATATATATATATATATATATATATATATATATATATATATATATATATTTTGAGAATTGAAGATTACTCAATTGACCATGGCTACAGTGACTGAAAAAGAAACATCCAATGCCTTAATTGAATATCCTAGAAAGTGATTCACTAGAAAGTATCAACCAAATCGTCCACGCCCTTTCGTTTTCAAATCTCACCTATAAATTAGTTAACGCCACTGCCATCCTCAACCAAGATGCACTCACGAATTTAACTAACAAAAACGCCATGGAAATGAACCTCACCTACCTACTCCTCCTCTATGCTTCCCTCTCTTTTCTCTCTCTTCTTTTTGCCTTCAATCTCATCCGTCCATCGCTTCCCGTTCGCAAAAACTTGCCGCCGAGACTCTTCGGTTGACTCGTGGCGCTCCAATGTTGCTACCCCATTGTGCAGCCAATGACTGCACAATAGGTGGATATAACGTGCCACGTGACACAATGGTACTGCATACACAGGGATCCAAAGGAATGGGAGGAGCCCACAAGTTTCAGACCAGAGCGATATGAAAAGTCAGAAGTGGAGGTACAGAAGTTGGTAGCTTTCGGAGTGGGGAGGCGAGCTTGTCCTGGGTCTGCCATGGCACAAAGGGTTATGGGTCTAACTTTGGCAGCATTGATTCAGTGCTTCGAGTGGGAGAGAATTGGAGAAGAACAAGTCGACATGAATGAGGGGACTGGTGCCACAATGCCCAAGATTGTGCCATTGGAGGCCATGTGTGCAAACCTCGTCCCATCGTGGAAAACTTCTTTCACTGAGAAATAA

mRNA sequence

ATGGAATTAGACACTTACTTCCTCTACTCTTCCCTCTCGTTTCTCTTTCTTCTTTTCGCTTTCAACCTCATCCGTCGATCCCTTCGATTTCGCAACAACTTACCGCCGAGTCCGCCATCTCTTCCGGTCATCGGCCATCTCCATCTCTTGAAAAAGCCACTCCACCGGAATTTCCAGAACCTCTCCGCCAAATATGGCCCTGTTATGTCTCTCCGCCTCGGATCTCGCCTTGCAGTCGTTGTATCCTCTTCGTCGGCGGTGGAGGACTGCTTCACTAAGAACGACGTCGTTCTCGCCAACCGTCCTCGCTTGCTAATCGGCAAATACCTCGGCTACAACTACACTACCATGATTGGGGCTGCGTACGGCGACCACTGGCGGAACCTCCGCCGAATCGGCTCAATCGAAATCTTCTCTTTGTCTCGAATCAACAAATTCGCCGAAATTCGGAGGGACGAAGTGGAGCGATTGGTGCGGAAACTTTCGAGCAATTCGATCCATCAATTCTCGAAAGTAGAAATGCAATCGGCGTTGTTGGAGCTGACGTTCAACATCTCGATGAGAATGGTGGCCGGAAAGAGGTATTACGGCGACGAGGTGACGGACGAGGAAGAGGCGAGGCAGTTCAGGGAGTTGATTAAGCAGATTGTGGCGCTGGGAGGAGTATCAAATCCAGGGGATTTTTTGCCCATTCTGAACTGGATTTCGAAGGGTTTCGAGAGGAAATTGATTCAATTTGGGAAGAAGATGGATGCGTTCTTGCAGGGGCTGATCGACGACCACCTGAGGGAGAAGGAAGAGGGAAGGAACACGATGATCGATCACCTGCTCTCTCTGCAAGAATCGGATCCTGCTCAATACGACGACCAAATAATCAAAGGATTTATACTGGTGTTACTAACTGCTGGGACCGACACATCAGCTGTGACAATGGAATGGGCCTTGTCTCATCTGCTGAACAATCCTGAAGTGTTAAAGAAAGCAAAAGACGAACTAGACACTCAAATTGGACAAGAACGGCTCGTTGAAGAACCAGACATGTCCAAACTACCCTATCTCCAGGGAATCATCTCTGAGACTCTTCGATTGACTCCCGCCGTTCCAATGTTGCTGCCCCATTATGCAGCCAATGACTGCACAATAGGTGGATATAACGTGCCACGTGACACGATGGTACTAGTTAATGCATGGGCCATACATAGGGATCCAAAGGAATGGGAGAATCCCACAAGTTTCGTACCAGAACGATATGAAAATTCGTCAGACGAAGTTAAGGTACACAAGTTGATACCTTTCGGAGTAGGGAGGCGAGCTTGTCCTGGGTATGCTATGGCACAAAGAGTGATGGGTCTAACTTTGGCAACACTGGTTCAGTGCTTCGAGTGGGAGAGAGTTGGAGAAGAAAAAGTGGACATGCACGAGGGTGCTGGTGCCACAATGCCCAAGCTTCTGCCATTGGAGGCTATGTGCAAAGCTCGTCCCATCGTCAACAACCTTTTTGAGGCGTTTCAGGACAAACCAAGCGGAACCGGGTGGCCAGAGGCGGTAGGGACTAAACGGAGCCGGAGGGACTCGGCCTCGGCCATGCCCGACCCCTTAGGTCTTGGCCCGTCATGTTTGCCGGTTTCGTCTATAGGATCCATCTCCCAGCCTTATTTCTGTCTGGTTGTCATCGTCAGCTCCTTGGATCCAAAGGAATGGGAGGAGCCCACAAGTTTCAGACCAGAGCGATATGAAAAGTCAGAAGTGGAGGTACAGAAGTTGGTAGCTTTCGGAGTGGGGAGGCGAGCTTGTCCTGGGTCTGCCATGGCACAAAGGGTTATGGGTCTAACTTTGGCAGCATTGATTCAGTGCTTCGAGTGGGAGAGAATTGGAGAAGAACAAGTCGACATGAATGAGGGGACTGGTGCCACAATGCCCAAGATTGTGCCATTGGAGGCCATGTGTGCAAACCTCGTCCCATCGTGGAAAACTTCTTTCACTGAGAAATAA

Coding sequence (CDS)

ATGGAATTAGACACTTACTTCCTCTACTCTTCCCTCTCGTTTCTCTTTCTTCTTTTCGCTTTCAACCTCATCCGTCGATCCCTTCGATTTCGCAACAACTTACCGCCGAGTCCGCCATCTCTTCCGGTCATCGGCCATCTCCATCTCTTGAAAAAGCCACTCCACCGGAATTTCCAGAACCTCTCCGCCAAATATGGCCCTGTTATGTCTCTCCGCCTCGGATCTCGCCTTGCAGTCGTTGTATCCTCTTCGTCGGCGGTGGAGGACTGCTTCACTAAGAACGACGTCGTTCTCGCCAACCGTCCTCGCTTGCTAATCGGCAAATACCTCGGCTACAACTACACTACCATGATTGGGGCTGCGTACGGCGACCACTGGCGGAACCTCCGCCGAATCGGCTCAATCGAAATCTTCTCTTTGTCTCGAATCAACAAATTCGCCGAAATTCGGAGGGACGAAGTGGAGCGATTGGTGCGGAAACTTTCGAGCAATTCGATCCATCAATTCTCGAAAGTAGAAATGCAATCGGCGTTGTTGGAGCTGACGTTCAACATCTCGATGAGAATGGTGGCCGGAAAGAGGTATTACGGCGACGAGGTGACGGACGAGGAAGAGGCGAGGCAGTTCAGGGAGTTGATTAAGCAGATTGTGGCGCTGGGAGGAGTATCAAATCCAGGGGATTTTTTGCCCATTCTGAACTGGATTTCGAAGGGTTTCGAGAGGAAATTGATTCAATTTGGGAAGAAGATGGATGCGTTCTTGCAGGGGCTGATCGACGACCACCTGAGGGAGAAGGAAGAGGGAAGGAACACGATGATCGATCACCTGCTCTCTCTGCAAGAATCGGATCCTGCTCAATACGACGACCAAATAATCAAAGGATTTATACTGGTGTTACTAACTGCTGGGACCGACACATCAGCTGTGACAATGGAATGGGCCTTGTCTCATCTGCTGAACAATCCTGAAGTGTTAAAGAAAGCAAAAGACGAACTAGACACTCAAATTGGACAAGAACGGCTCGTTGAAGAACCAGACATGTCCAAACTACCCTATCTCCAGGGAATCATCTCTGAGACTCTTCGATTGACTCCCGCCGTTCCAATGTTGCTGCCCCATTATGCAGCCAATGACTGCACAATAGGTGGATATAACGTGCCACGTGACACGATGGTACTAGTTAATGCATGGGCCATACATAGGGATCCAAAGGAATGGGAGAATCCCACAAGTTTCGTACCAGAACGATATGAAAATTCGTCAGACGAAGTTAAGGTACACAAGTTGATACCTTTCGGAGTAGGGAGGCGAGCTTGTCCTGGGTATGCTATGGCACAAAGAGTGATGGGTCTAACTTTGGCAACACTGGTTCAGTGCTTCGAGTGGGAGAGAGTTGGAGAAGAAAAAGTGGACATGCACGAGGGTGCTGGTGCCACAATGCCCAAGCTTCTGCCATTGGAGGCTATGTGCAAAGCTCGTCCCATCGTCAACAACCTTTTTGAGGCGTTTCAGGACAAACCAAGCGGAACCGGGTGGCCAGAGGCGGTAGGGACTAAACGGAGCCGGAGGGACTCGGCCTCGGCCATGCCCGACCCCTTAGGTCTTGGCCCGTCATGTTTGCCGGTTTCGTCTATAGGATCCATCTCCCAGCCTTATTTCTGTCTGGTTGTCATCGTCAGCTCCTTGGATCCAAAGGAATGGGAGGAGCCCACAAGTTTCAGACCAGAGCGATATGAAAAGTCAGAAGTGGAGGTACAGAAGTTGGTAGCTTTCGGAGTGGGGAGGCGAGCTTGTCCTGGGTCTGCCATGGCACAAAGGGTTATGGGTCTAACTTTGGCAGCATTGATTCAGTGCTTCGAGTGGGAGAGAATTGGAGAAGAACAAGTCGACATGAATGAGGGGACTGGTGCCACAATGCCCAAGATTGTGCCATTGGAGGCCATGTGTGCAAACCTCGTCCCATCGTGGAAAACTTCTTTCACTGAGAAATAA

Protein sequence

MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQNLSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFERKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLLTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISETLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENSSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATMPKLLPLEAMCKARPIVNNLFEAFQDKPSGTGWPEAVGTKRSRRDSASAMPDPLGLGPSCLPVSSIGSISQPYFCLVVIVSSLDPKEWEEPTSFRPERYEKSEVEVQKLVAFGVGRRACPGSAMAQRVMGLTLAALIQCFEWERIGEEQVDMNEGTGATMPKIVPLEAMCANLVPSWKTSFTEK

Homology

[Close]

BLAST of Lag0013107 vs. NCBI nr
Match: XP_022949637.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 857.8 bits (2215), Expect = 6.1e-245
Identity = 423/501 (84.43%), Postives = 460/501 (91.82%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MDLHT-FLYSFFSILFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM AGKRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKL + GKK+DAFLQGLIDDH R+KEEGRNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWAL HLLNNPEVLKKA+DE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FG+GRRACPG AMA R+MGLTL  L+QCFEWE++G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEWEKIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVNNLFE 502
            K++PLEAMC+ARP V+NL +
Sbjct: 481 TKMVPLEAMCRARPTVDNLLD 498

[Close]

BLAST of Lag0013107 vs. NCBI nr
Match: KAG7034317.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 856.7 bits (2212), Expect = 1.3e-244
Identity = 424/501 (84.63%), Postives = 459/501 (91.62%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MNLHT-FLYSFFSILFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM AGKRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKL + GKK+DAFLQGLIDDH R+KEEGRNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWAL HLLNNPEVLKKA+DE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPIEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FGVGRRACPG A+A R+MGLTL  L+QCFEWER+G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGVGRRACPGSAVAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVNNLFE 502
            K++PLEAMC+ARP V+NL +
Sbjct: 481 TKMVPLEAMCRARPTVDNLLD 498

[Close]

BLAST of Lag0013107 vs. NCBI nr
Match: XP_022977458.1 (cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 856.3 bits (2211), Expect = 1.8e-244
Identity = 426/497 (85.71%), Postives = 459/497 (92.35%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MDLHT-FLYSFFSPLFLLFAFNLITRSLRPRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM AGKRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLI+ GKK+DAFLQGLIDDH R+KEEGRNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWALSHLLNNPEVLKKAKDE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FGVGRRACPG AMA R+MGLTL  L+QCFEWER+G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVN 498
            K++PLEAMC+ARP V+
Sbjct: 481 TKMVPLEAMCRARPTVD 494

[Close]

BLAST of Lag0013107 vs. NCBI nr
Match: XP_023543941.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855.1 bits (2208), Expect = 3.9e-244
Identity = 424/501 (84.63%), Postives = 459/501 (91.62%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MDLHT-FLYSFFSTLFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM A KRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAVKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLI+ GKK+DAFLQGLIDDH R+KEE RNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLIELGKKVDAFLQGLIDDHRRKKEESRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWAL HLLNNPEVLKKA+DE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FGVGRRACPG AMA R+MGLTL  L+QCFEWER+G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVNNLFE 502
            K++PLEAMC+ARP V+NL +
Sbjct: 481 TKMVPLEAMCRARPTVDNLLD 498

[Close]

BLAST of Lag0013107 vs. NCBI nr
Match: XP_004141996.2 (cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_003503 [Cucumis sativus])

HSP 1 Score: 833.9 bits (2153), Expect = 9.4e-238
Identity = 402/500 (80.40%), Postives = 454/500 (90.80%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+LDTY LYSSLS  FLL AF  I RSLR RNNLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS+LAVV+SSS+AVEDCFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
            YGDHWRNLRRIG+IEIFS+SRINKF +IRRDEV R+VRKLS NSIHQFSKVE+QSA+ E
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           LTFNI+MRM AGKRYYG++VT+EEEAR+FRELIK+IVA+GGVSNPGDFLP+LNW+SKG E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLIQ GKK+DAFLQGLIDDH + KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAG+DTSAVTMEWALSHLLNNP+VLKKAK+E+DTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRL PA PML+PHYA NDC I GY+VPRDTMVLVNAWAIHRD  EWE+  SF PERYE  
Sbjct: 361 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYE-K 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
           S+ V++HK +PFG+GRRACPG AMA RVMGLTLATL+QCFEW+++GEE +DM E +GA M
Sbjct: 421 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIM 480

Query: 481 PKLLPLEAMCKARPIVNNLF 501
            K++PLEAMCKARPI+NN+F
Sbjct: 481 TKMVPLEAMCKARPIINNIF 499

[Close]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 9.3e-164
Identity = 289/501 (57.68%), Postives = 375/501 (74.85%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPP-SLPVIGHLHLLKKPLHRNFQ 60
           ME  T  LY+ L+ + L  +  L   S R R NLPPSP  +LPVIGHLHL+ K LHR+  
Sbjct: 1   MESST-LLYTFLAVVLLSISLKLFPVSRR-RRNLPPSPGLALPVIGHLHLIGKLLHRSLY 60

Query: 61  NLSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIG 120
           +LS KYG V SL+LG+RL +VVSS +A E+CFTKND+V ANRP  ++GKY+GYNYTTM+G
Sbjct: 61  DLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVG 120

Query: 121 AAYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALL 180
           + YG+HWRNLRR+ ++EIFS   +N+F  IR DEV++L+  L  +S   F KVEM+S L 
Sbjct: 121 SPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLS 180

Query: 181 ELTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWIS-KG 240
           EL+FN++MRMVAGKRY+G +V D +EA+ FR LI ++    G SNPGDF+P L WI  K 
Sbjct: 181 ELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKN 240

Query: 241 FERKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILV 300
           +E+K+ +  ++MDAFLQ LI  H     +   TMIDHLLSLQES P  Y DQIIKG I+V
Sbjct: 241 YEKKVSKISQEMDAFLQRLI--HESRINKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMV 300

Query: 301 LLTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIIS 360
           LL AGTDTSAVT+EWA+S LLN+PE L+KA+ E++TQ+G  RL+EE D+ KL YL  IIS
Sbjct: 301 LLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIIS 360

Query: 361 ETLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYE 420
           ET RL PA PML+PH +++DC + GY+VP+ T++LVNAWAIHRDP+ W+ PT F PER+ 
Sbjct: 361 ETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH- 420

Query: 421 NSSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGA 480
               E++  KL+PFG+GRR+CPG  +AQRV+GLTL  L+QCFEW+R+GE K+DM EG+G 
Sbjct: 421 -GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGL 480

Query: 481 TMPKLLPLEAMCKARPIVNNL 500
           TMPK  PLEA+CK R I++ +
Sbjct: 481 TMPKAQPLEALCKPRNILHKV 494

[Close]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 4.6e-163
Identity = 282/500 (56.40%), Postives = 367/500 (73.40%), Query Frame = 0


Query: 7   FLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQNLSAKYG 66
           F YS LS  F++    L++ + R   NLPP PP++P+IG+LH LK PLHR F  LS  YG
Sbjct: 3   FYYSLLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 62

Query: 67  PVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHW 126
            + SL  GSRL VVVSS S   +CFTKND++LANRPR L GKY+ YNYTT+  A+YGDHW
Sbjct: 63  DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 122

Query: 127 RNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQ---FSKVEMQSALLELTF 186
           RNLRRI +I++ S +R+N F  +RRDE  RL++KL  + + +   F+KVE++  L E+TF
Sbjct: 123 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 182

Query: 187 NISMRMVAGKRYYGD--EVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWIS-KGFE 246
           N  MRM++GKRYYGD  +V+D EEA+QFRE+I ++++L G +N GDFLP+L  +     E
Sbjct: 183 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 242

Query: 247 RKLIQFGKKMDAFLQGLIDDHLREK-EEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVL 306
           ++  +  K+ +AFL+GLI++H R        TMIDHLL L ES P  Y D +IKG I  +
Sbjct: 243 KRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGM 302

Query: 307 LTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISE 366
           L AGTDTSAVT+EW +S LLN+PEVLKKAK+ELDTQIG+ +LV+E D+SKLPYLQ IISE
Sbjct: 303 LLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISE 362

Query: 367 TLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYEN 426
           TLRL P  P+LLPHY++ DCTIG +NVP+DT++L N W IHRDPK W +  SF PER+E 
Sbjct: 363 TLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEK 422

Query: 427 SSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGAT 486
              E +V+K++ FG+GRRACPG ++AQR +G T+  L+QCFEWER  EEK+DM EG G T
Sbjct: 423 ---EEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGIT 482

Query: 487 MPKLLPLEAMCKARPIVNNL 500
           MP  +PL AMCKA PI N++
Sbjct: 483 MPMKIPLRAMCKALPIANDV 499

[Close]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 568.9 bits (1465), Expect = 7.4e-161
Identity = 274/504 (54.37%), Postives = 380/504 (75.40%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           ME+ +   Y S+ +L L F FN++ R+ +F+ NLPP PPSLP+IG+LH LK+PLHR F+ 
Sbjct: 1   MEILSLLSY-SVFYLALFFIFNIVIRARKFK-NLPPGPPSLPIIGNLHHLKRPLHRTFKG 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LS KYG V SL  GSRL VVVSS+S  + CFTKNDVVLANRPR L GKY+ YNYTT+   
Sbjct: 61  LSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGST 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YG+HWRNLRRI ++++ S  RIN F+ IRRDE +RL+ +L+ +S   F+++E+ S L +
Sbjct: 121 SYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYD 180

Query: 181 LTFNISMRMVAGKRYYGD--EVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWIS-K 240
           +TFN  MRM++GKRYYG+  + +D +EA QFR+++ +++ L G +N  DF+P+L ++  +
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 241 GFERKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFIL 300
             E++L     K DAFL+GLI++H R K+E  NTMIDHLL+LQ+S P  Y DQIIKG  L
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEH-RTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLAL 300

Query: 301 VLLTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGII 360
            +L AGTD+SAVT+EW++S+LLN+PEVLKK KDELDT +GQ+RLV+E D+ KL YL+ +I
Sbjct: 301 AMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVI 360

Query: 361 SETLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERY 420
           +ETLRL    P+LLPH  +++C IGGY VP+DT+VL+NAWAIHRDP+ W   T+F PER+
Sbjct: 361 NETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF 420

Query: 421 ENSSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAG 480
           E   +   + KLI FG+GRRACPG  +A R + +TLA L+QCF+W+ +  +K+D+ E  G
Sbjct: 421 EKKGE---LEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDG 480

Query: 481 ATMPKLLPLEAMCKARPIVNNLFE 502
            T+ KL+PL+AMCK+RP++N +F+
Sbjct: 481 FTLTKLVPLKAMCKSRPVINKVFK 498

[Close]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.6e-160
Identity = 281/503 (55.86%), Postives = 370/503 (73.56%), Query Frame = 0


Query: 3   LDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQNLS 62
           + T++L   +S  FL+    +   + R   NLPP P  LP+IG+LH LK+PLH  F  LS
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLS 60

Query: 63  AKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAY 122
            KYG + SL  GSRL VVVSS +  ++CFTKND+VLANRP  L GKY+GYN TT+  + Y
Sbjct: 61  QKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPY 120

Query: 123 GDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELT 182
           GDHWRNLRRI SIEI S  R+N F EIRRDE+ RL++KL+  S + F++VE++    E+T
Sbjct: 121 GDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMT 180

Query: 183 FNISMRMVAGKRYYGD--EVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWIS-KGF 242
           FN  MRMV+GKRYYG+  +V+D EEAR FR +IK++V+LGG +N GDFL  L W    G 
Sbjct: 181 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGL 240

Query: 243 ERKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVL 302
           E++L +  K+ DAFLQGLID+H R  +   NTMIDHLL+ Q+S P  Y DQIIKG ++V+
Sbjct: 241 EKRLKKISKRTDAFLQGLIDEH-RFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVM 300

Query: 303 LTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISE 362
           L AGTDTS+VT+EWA+S+LLN+PE++KKAK+ELDT IG +R V+E D+SKLPYLQ I+ E
Sbjct: 301 LLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYE 360

Query: 363 TLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYEN 422
           TLRL  A P+L+PH ++ D ++GGYN+P++T+++VNAW IHRDP  W +PT F PER+E 
Sbjct: 361 TLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK 420

Query: 423 SSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGAT 482
              E +V+KL+ FG+GRRACPG  ++QR  GLTL  L+QCFEW+R+GEEK+DM E  G T
Sbjct: 421 ---EGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGIT 480

Query: 483 MPKLLPLEAMCKAR-PI-VNNLF 501
             K   L AMCK R P+ +N++F
Sbjct: 481 AGKKTSLNAMCKVRHPLKINDVF 499

[Close]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.5e-158
Identity = 269/498 (54.02%), Postives = 372/498 (74.70%), Query Frame = 0


Query: 7   FLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQNLSAKYG 66
           +L  SL +L + F   L+ +S +F+ NLPP P SLP+IG+LH LK+PL+R F+ L+ KYG
Sbjct: 6   YLCYSLFYLSIFFIIRLLFQSRKFK-NLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYG 65

Query: 67  PVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHW 126
            V+SL  GSRL VVVSS S  ++CFTKNDVVLANRPR L GKY+ YNYTT+   +YG+HW
Sbjct: 66  NVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHW 125

Query: 127 RNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELTFNIS 186
           RNLRRI S+++ S  RIN FA IRRDE +RL++KL+ +S  +F++VE+     ++TFN  
Sbjct: 126 RNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNI 185

Query: 187 MRMVAGKRYYGD--EVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWIS-KGFERKL 246
           MRM++GKRYYGD  ++++ +EA QFR+++ +++ L G +N  DF+P+L ++  +  E+++
Sbjct: 186 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 245

Query: 247 IQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLLTAG 306
            + G+K D FL GL+ +  R K+E  NTMIDHLL++QES P  Y D IIKG  L +L AG
Sbjct: 246 KRIGEKNDVFLSGLLQEQ-RSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAG 305

Query: 307 TDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISETLRL 366
           TD+SAVT+EW +S++LN PEVLKK +DE+DT +GQ+RLV+E D+ KL YL+ +I ETLRL
Sbjct: 306 TDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRL 365

Query: 367 TPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENSSDE 426
               P+LLPH  A++C +GGY VPRDT+VL+NAWAIHRDP+ W   T+F PER++   + 
Sbjct: 366 YTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGE- 425

Query: 427 VKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATMPKL 486
             + K+I FG+GRRACPG  +A R + +TLA LVQCF+W+R+ +EK+DM E  G TM KL
Sbjct: 426 --LEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKL 485

Query: 487 LPLEAMCKARPIVNNLFE 502
           LPL+AMCK RP+VN +F+
Sbjct: 486 LPLKAMCKTRPVVNKVFK 498

[Close]

BLAST of Lag0013107 vs. ExPASy TrEMBL
Match: A0A6J1GCP2 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=1)

HSP 1 Score: 857.8 bits (2215), Expect = 2.9e-245
Identity = 423/501 (84.43%), Postives = 460/501 (91.82%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MDLHT-FLYSFFSILFLLFAFNLITRSLRLRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM AGKRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKL + GKK+DAFLQGLIDDH R+KEEGRNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLFELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWAL HLLNNPEVLKKA+DE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALCHLLNNPEVLKKARDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTIHGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FG+GRRACPG AMA R+MGLTL  L+QCFEWE++G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGLGRRACPGSAMAHRIMGLTLGALIQCFEWEKIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVNNLFE 502
            K++PLEAMC+ARP V+NL +
Sbjct: 481 TKMVPLEAMCRARPTVDNLLD 498

[Close]

BLAST of Lag0013107 vs. ExPASy TrEMBL
Match: A0A6J1IQ18 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 8.5e-245
Identity = 426/497 (85.71%), Postives = 459/497 (92.35%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+L T FLYS  S LFLLFAFNLI RSLR R NLPPSPPSLPVIGHLHLLKKPLHRNFQN
Sbjct: 1   MDLHT-FLYSFFSPLFLLFAFNLITRSLRPRKNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS LAVVVSSSSAVE+CFTKNDVVLANRPRLLIGKYLGYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSNLAVVVSSSSAVEECFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
           +YGDHWRNLRRIG+IEIFS SRINKFA+IRRDEV+RLVRKLS NSIHQ+SKVEMQSAL E
Sbjct: 121 SYGDHWRNLRRIGAIEIFSSSRINKFADIRRDEVKRLVRKLSRNSIHQYSKVEMQSALSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           +TFNISMRM AGKRYYG++VTD+EEAR+FRELIK+IVA GGVSNPGDF PILNWISKGFE
Sbjct: 181 MTFNISMRMAAGKRYYGEDVTDKEEARRFRELIKEIVAAGGVSNPGDFFPILNWISKGFE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLI+ GKK+DAFLQGLIDDH R+KEEGRNTMIDHLLSLQES PA YDDQIIKGFILVLL
Sbjct: 241 RKLIELGKKVDAFLQGLIDDHRRKKEEGRNTMIDHLLSLQESQPAVYDDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAGTDTSAVT+EWALSHLLNNPEVLKKAKDE+DTQIGQERLVEEPD+SKLPYLQGIISET
Sbjct: 301 TAGTDTSAVTVEWALSHLLNNPEVLKKAKDEIDTQIGQERLVEEPDVSKLPYLQGIISET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRLTPA PMLLPHYAANDCTI GY+VPRDTM+LVNAWAIHRDP EWE+P SF PERYE S
Sbjct: 361 LRLTPAAPMLLPHYAANDCTICGYDVPRDTMLLVNAWAIHRDPNEWEDPLSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
             EV+VHKL+ FGVGRRACPG AMA R+MGLTL  L+QCFEWER+G E +DM+E  GATM
Sbjct: 421 --EVEVHKLLSFGVGRRACPGSAMAHRIMGLTLGALIQCFEWERIGAEDLDMNERVGATM 480

Query: 481 PKLLPLEAMCKARPIVN 498
            K++PLEAMC+ARP V+
Sbjct: 481 TKMVPLEAMCRARPTVD 494

[Close]

BLAST of Lag0013107 vs. ExPASy TrEMBL
Match: A0A0A0KFV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 4.5e-238
Identity = 402/500 (80.40%), Postives = 454/500 (90.80%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+LDTY LYSSLS  FLL AF  I RSLR RNNLPPSPPS PVIGHLHLLKKP+HR F N
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPIHRTFSN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS+LAVV+SSS+AVEDCFTKNDVVLANRPRLL+GKY+GYNYTTMIGA
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
            YGDHWRNLRRIG+IEIFS+SRINKF +IRRDEV R+VRKLS NSIHQFSKVE+QSA+ E
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           LTFNI+MRM AGKRYYG++VT+EEEAR+FRELIK+IVA+GGVSNPGDFLP+LNW+SKG E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLIQ GKK+DAFLQGLIDDH + KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFIL+LL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAG+DTSAVTMEWALSHLLNNP+VLKKAK+E+DTQIGQERLVEE D+SKLPYLQ I+SET
Sbjct: 301 TAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRL PA PML+PHYA NDC I GY+VPRDTMVLVNAWAIHRD  EWE+  SF PERYE  
Sbjct: 361 LRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYE-K 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
           S+ V++HK +PFG+GRRACPG AMA RVMGLTLATL+QCFEW+++GEE +DM E +GA M
Sbjct: 421 SEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIM 480

Query: 481 PKLLPLEAMCKARPIVNNLF 501
            K++PLEAMCKARPI+NN+F
Sbjct: 481 TKMVPLEAMCKARPIINNIF 499

[Close]

BLAST of Lag0013107 vs. ExPASy TrEMBL
Match: A0A1S3B0W6 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 1.4e-231
Identity = 395/499 (79.16%), Postives = 444/499 (88.98%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+LDTY LYSSLS  FLL AF  I RSLR RNNLPPSPPS PVIGHLHLLKKP HR F N
Sbjct: 1   MDLDTYVLYSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPPHRTFLN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS+LAVV+SSS+AVE+CFTKNDVVLANRPRLLIGKY+GYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
            YGDHWRNLRRIG+IEIFS+SRINKF +IRRDEV R+VRKLS NSIHQFSKVE+QSA+ E
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           LTFNI+MRM AGKRYYG++VT+EEEAR+FR LIK+IVA+GGVSNPGDFLP+LNW+SKG E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLIQ GKK+DAFLQGLIDDH   KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFILVLL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAG+DTSAVTMEWAL HLLNNP+VLK A+DELDTQIGQERLVEE D+ KLPYLQ II ET
Sbjct: 301 TAGSDTSAVTMEWALCHLLNNPQVLKMARDELDTQIGQERLVEESDIPKLPYLQRIIYET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRL PA PML+PHYA+NDC I GY+VPR+TMVL+NAWAIHRD  EWE+  SF PERYE S
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
              ++VHK +PFGVGRRACPG AMA RVMGLTLATL+QCFEW+++GEE +DM E +GA M
Sbjct: 421 K-AMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALM 480

Query: 481 PKLLPLEAMCKARPIVNNL 500
            K++PLEAMCK RPI+NN+
Sbjct: 481 TKMVPLEAMCKTRPIINNI 498

[Close]

BLAST of Lag0013107 vs. ExPASy TrEMBL
Match: A0A5D3CLS1 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004450 PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 7.7e-230
Identity = 393/499 (78.76%), Postives = 443/499 (88.78%), Query Frame = 0


Query: 1   MELDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPSLPVIGHLHLLKKPLHRNFQN 60
           M+LDTY L SSLS  FLL AF  I RSLR RNNLPPSPPS PVIGHLHLLKKPLHR F N
Sbjct: 1   MDLDTYVLCSSLSLFFLLLAFKFISRSLRLRNNLPPSPPSFPVIGHLHLLKKPLHRTFLN 60

Query: 61  LSAKYGPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 120
           LSAKYGPVMSLRLGS+LAVV+SSS+AVE+CFTKNDVVLANRPRLLIGKY+GYNYTTM+ A
Sbjct: 61  LSAKYGPVMSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAA 120

Query: 121 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 180
            YGDHWRNLRRIG+IEIFS+SRINKF +IRRDEV R+VRKLS NSIHQFSKVE+QSA+ E
Sbjct: 121 PYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSE 180

Query: 181 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 240
           LTFNI+MRM AGKRYYG++VT+EEEAR+FR LIK+IVA+GGVSNPGDFLP+LNW+SKG E
Sbjct: 181 LTFNITMRMAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIE 240

Query: 241 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 300
           RKLIQ GKK+DAFLQGLIDDH   KEEGRNTMIDHLLSLQ+S+P  Y+DQIIKGFILVLL
Sbjct: 241 RKLIQLGKKVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLL 300

Query: 301 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 360
           TAG+DTSA+TMEWAL HLLNNP+VLKKA+DELDTQIGQE LVEE D+ KLPYLQ II ET
Sbjct: 301 TAGSDTSAMTMEWALCHLLNNPQVLKKARDELDTQIGQEILVEESDIPKLPYLQRIIYET 360

Query: 361 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 420
           LRL PA PML+PHYA+NDC I GY+VPR+TMVL+NAWAIHRD  EWE+  SF PERYE S
Sbjct: 361 LRLNPAAPMLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKS 420

Query: 421 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 480
              ++VHK +PFGVGRRACPG AMA RVMGLTLATL+QCFEW+++GEE +DM E +GA M
Sbjct: 421 K-AMEVHKFLPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALM 480

Query: 481 PKLLPLEAMCKARPIVNNL 500
            K++PLEAMCK R I+NN+
Sbjct: 481 TKMVPLEAMCKTRSIINNI 498

[Close]

BLAST of Lag0013107 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 569.7 bits (1467), Expect = 3.1e-162
Identity = 283/491 (57.64%), Postives = 367/491 (74.75%), Query Frame = 0


Query: 13  SFLFLLFAFNLIRRSLRFRNNLPPSPP-SLPVIGHLHLLKKPLHRNFQNLSAKY--GPVM 72
           S LF++ +   +   L+ + NLPPSP  SLPVIGHL LLK P+HR F +LS      P+ 
Sbjct: 9   SILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIF 68

Query: 73  SLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHWRNL 132
           SLRLG+RL  V SS S  E+CFTKNDVVLANRP  ++ K++ Y+YTTMI A+YGDHWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 133 RRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELTFNISMRM 192
           RRIGS+EIFS  R+N F  IR+DE+ RLV +LS N   +F KV+M+S L +LTFN  +RM
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 193 VAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFERKLIQFGKK 252
           VAGKRYYGD V D+ EA++ R+LI  +VA  G  N  D+LP+L  +S  +E ++ +   +
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS-DYETRVKKLAGR 248

Query: 253 MDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLLTAGTDTSAV 312
           +D FLQGL+D+    KE+G NTMIDHLL+LQES P  + D+IIKG +L L+ AGTDTSAV
Sbjct: 249 LDEFLQGLVDEKREAKEKG-NTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAV 308

Query: 313 TMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISETLRLTPAVPM 372
           T+EWALS++LN+P+VL KA+DE+D +IG +RL++E D+S LPYLQ I+SETLRL PA PM
Sbjct: 309 TLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPM 368

Query: 373 LLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENSSDEVKVHKL 432
           LLPH A+ DC + GY++PR T++L N WAIHRDP+ W++P SF PER+E    E +  KL
Sbjct: 369 LLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---EGEAQKL 428

Query: 433 IPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATMPKLLPLEAM 492
           +PFG+GRRACPG  +A R++ LTL +L+QC EWE++GEE VDM EG G TMPK  PLEAM
Sbjct: 429 MPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAM 488

Query: 493 CKARPIVNNLF 501
           C+ARP V  +F
Sbjct: 489 CRARPSVVKIF 493

[Close]

BLAST of Lag0013107 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 568.5 bits (1464), Expect = 6.8e-162
Identity = 280/491 (57.03%), Postives = 363/491 (73.93%), Query Frame = 0


Query: 13  SFLFLLFAFNLIRRSLRFRNNLPPSPP-SLPVIGHLHLLKKPLHRNFQNLSAKYG--PVM 72
           +FLF+  +   I   ++ R NLPPSP  +LPVIGHL LLK PLHR F ++S   G  P++
Sbjct: 9   TFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPII 68

Query: 73  SLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHWRNL 132
           SLRLG+RL  VVSS S  E+CFTKNDVVLANR   L  K++ Y  TT++ A+YGDHWRNL
Sbjct: 69  SLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNL 128

Query: 133 RRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELTFNISMRM 192
           RRIG++EIFS  R+N F+ IRRDE+ RL+  LS NS  +F+KVEM+S    LTFN  +RM
Sbjct: 129 RRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRM 188

Query: 193 VAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFERKLIQFGKK 252
           +AGK YYGD   D+ EA++ RELI + +   G  N  D+LPIL WI+ G E+++ +   +
Sbjct: 189 LAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWIT-GSEKRIKKIASR 248

Query: 253 MDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLLTAGTDTSAV 312
           +D FLQGL+D+    KE+ +NTM+DHLL LQE+ P  Y D IIKG +L L+ AGTDTSAV
Sbjct: 249 LDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAV 308

Query: 313 TMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISETLRLTPAVPM 372
           T+EW LS LLN+P++L KA+DE+D ++G  RLVEE D+S LPYLQ I+SE+LRL PA P+
Sbjct: 309 TLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPL 368

Query: 373 LLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENSSDEVKVHKL 432
           L+PH A+ DC +GGY++PR TM+L NAWAIHRDPK W++PTSF PER+E    E +  KL
Sbjct: 369 LVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEK---EGEAQKL 428

Query: 433 IPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGAT-MPKLLPLEA 492
           + FG+GRRACPG  +AQR+  LT+ +L+QCFEWER+GEE+VDM EG G   MPK +PL A
Sbjct: 429 LGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVA 488

Query: 493 MCKARPIVNNL 500
           MCKARP+V  +
Sbjct: 489 MCKARPVVGKI 495

[Close]

BLAST of Lag0013107 vs. TAIR 10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 558.9 bits (1439), Expect = 5.4e-159
Identity = 284/504 (56.35%), Postives = 363/504 (72.02%), Query Frame = 0


Query: 3   LDTYFLYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPP-SLPVIGHLHLLKKPLHRNFQNL 62
           ++T +L  SL F F+  +  L+      + NLPPSP    P+IGHLHLLK PLHR F +L
Sbjct: 8   METIYLILSLFFFFIFLSLKLLFGPRPRKLNLPPSPSRPFPIIGHLHLLKLPLHRRFLSL 67

Query: 63  SAKY--GPVMSLRLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIG 122
           S       + SL LGSRL  VVSS +  E+CFTKNDVVLANRP  L+GK++GYN TTM+G
Sbjct: 68  SESLNNAKIFSLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVG 127

Query: 123 AAYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALL 182
           AAYGD WRNLRRIG+IEIFS  R+N F  IR+DE+ RL+  L+ NS H F KVEM+   +
Sbjct: 128 AAYGDSWRNLRRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFM 187

Query: 183 ELTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGF 242
            LT N  +RMVAGKR+YGD   ++ EA+  R+LI ++V  GG  N  D+ PIL +++  +
Sbjct: 188 CLTINNIIRMVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVT-NY 247

Query: 243 ERKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVL 302
           E+ + +   ++D FLQ L+++   EK +G NTMIDHLLSLQE+ P  Y D IIKG ILV+
Sbjct: 248 EKHVKKLAGRVDEFLQSLVNEKRVEKVKG-NTMIDHLLSLQETQPDYYTDVIIKGIILVM 307

Query: 303 LTAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISE 362
           + AGTDTSA T+EWA+S+LLN+PEVL+KAK E+D QIG +RLVEE D+ KLPYLQ I+SE
Sbjct: 308 ILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSE 367

Query: 363 TLRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYEN 422
           TLRL P  PMLLPH A+ DC + GY+VPR T++LVNAWAIHRDPK WE P  F PER+E 
Sbjct: 368 TLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEK 427

Query: 423 SSDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEG-AGA 482
             ++    KL+PFG+GRR+CPG  +AQR++ L L +LVQCFEWERV E+ +DM E   G 
Sbjct: 428 KGED---KKLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGT 487

Query: 483 TMPKLLPLEAMCKARPIVNNLFEA 503
           TM K   L+AMCKARPIV+ + +A
Sbjct: 488 TMRKATSLQAMCKARPIVHKVLDA 506

[Close]

BLAST of Lag0013107 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 556.6 bits (1433), Expect = 2.7e-158
Identity = 276/488 (56.56%), Postives = 358/488 (73.36%), Query Frame = 0


Query: 15  LFLLFAFNLIRRSLRFRNNLPPSPP-SLPVIGHLHLLKKPLHRNFQNLSAKYG--PVMSL 74
           L L+F    I+R L    NLPPSP  +LPVIGHL LLK PLHR F ++S   G  P++SL
Sbjct: 15  LSLIFLIGRIKRKL----NLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISL 74

Query: 75  RLGSRLAVVVSSSSAVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGAAYGDHWRNLRR 134
           RLG+RL  VVSS S  E+CFTKNDV+LANR   +  K++ Y  +T++ A+Y +HWRNLRR
Sbjct: 75  RLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRR 134

Query: 135 IGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLELTFNISMRMVA 194
           IG++EIFS  R+N F+ IRRDE+ RL+ +L  NS + F+KVEM+S   +LTFN  +RM+A
Sbjct: 135 IGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLA 194

Query: 195 GKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFERKLIQFGKKMD 254
           GK YYGD   D+ EA++ R LI + ++  G  N  D++PIL WI+   E ++ +   ++D
Sbjct: 195 GKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-ETRIKKLAGRLD 254

Query: 255 AFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLLTAGTDTSAVTM 314
            FLQGL+D+    KE+  NTM+DHLL LQE+ P  Y D+IIKG +L L+  GTDT+AVT+
Sbjct: 255 EFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTL 314

Query: 315 EWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISETLRLTPAVPMLL 374
           EWALS LLNNPEVL KA+DE+D  IG +RL+EE D+  LPYLQ I+SETLRL PA PMLL
Sbjct: 315 EWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLL 374

Query: 375 PHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENSSDEVKVHKLIP 434
           PH A+ DC +GGY++PR TM+L NAWAIHRDP  W++PTSF PER+E    E +  KL+P
Sbjct: 375 PHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK---EGEAKKLMP 434

Query: 435 FGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATMPKLLPLEAMCK 494
           FG+GRRACPG  +AQR++ L+L +L+QCFEWER+GEE+VDM EG G TMPK  PLEAMC+
Sbjct: 435 FGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCR 494

Query: 495 ARPIVNNL 500
           AR  V  +
Sbjct: 495 ARDFVGKI 494

[Close]

BLAST of Lag0013107 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 550.8 bits (1418), Expect = 1.5e-156
Identity = 276/499 (55.31%), Postives = 367/499 (73.55%), Query Frame = 0


Query: 8   LYSSLSFLFLLFAFNLIRRSLRFRNNLPPSPPS-LPVIGHLHLLKKPLHRNFQNLSAKY- 67
           LYS  S +FL+ +F  ++     + NLPPSPP  LP+IGHL LLK P+HR  ++ S    
Sbjct: 10  LYSIFSLIFLIISFKFLKPK---KQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLD 69

Query: 68  ----GPVMSLRLGSRLAVVVSSSS-AVEDCFTKNDVVLANRPRLLIGKYLGYNYTTMIGA 127
               G VMSLRLGSRL  VVSS   A E+CF KNDVVLANRP+++IGK++GYN T MI A
Sbjct: 70  HNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAA 129

Query: 128 AYGDHWRNLRRIGSIEIFSLSRINKFAEIRRDEVERLVRKLSSNSIHQFSKVEMQSALLE 187
            YGDHWRNLRR+ +IEIFS  R+N F  +R DEV RL+ +LS  +  + + VE++  L++
Sbjct: 130 PYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMD 189

Query: 188 LTFNISMRMVAGKRYYGDEVTDEEEARQFRELIKQIVALGGVSNPGDFLPILNWISKGFE 247
           LTFN  MRM+ GKRYYG+E TDEEEA++ R+L+  + A     N  D++PIL   S  +E
Sbjct: 190 LTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS-SYE 249

Query: 248 RKLIQFGKKMDAFLQGLIDDHLREKEEGRNTMIDHLLSLQESDPAQYDDQIIKGFILVLL 307
            ++ + G++ D FLQGLIDD   ++E G  TMIDHLL LQ+SD   Y DQIIKG IL+++
Sbjct: 250 NRVKKLGEETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIKGIILIMV 309

Query: 308 TAGTDTSAVTMEWALSHLLNNPEVLKKAKDELDTQIGQERLVEEPDMSKLPYLQGIISET 367
            AGT+TSAVT+EWALS+LLN+P+V+ KA+DE+D ++G +RL+EE D+S+LPYL+ I+ ET
Sbjct: 310 IAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLET 369

Query: 368 LRLTPAVPMLLPHYAANDCTIGGYNVPRDTMVLVNAWAIHRDPKEWENPTSFVPERYENS 427
           LRL PA P+L+PH A+ DC IG Y++PR T +LVNAWAIHRDP  W++P SF PER+E  
Sbjct: 370 LRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK- 429

Query: 428 SDEVKVHKLIPFGVGRRACPGYAMAQRVMGLTLATLVQCFEWERVGEEKVDMHEGAGATM 487
             E +  KL+ FG+GRRACPG  +AQR++GL L +L+QCFEWERVG  +VDM EG G T+
Sbjct: 430 --EEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTV 489

Query: 488 PKLLPLEAMCKARPIVNNL 500
           PK +PL+A+CKARP ++ +
Sbjct: 490 PKAIPLKAICKARPFLHKI 500

The following BLAST results are available for this feature:

BLAST of Lag0013107 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949637.1	6.1e-245	84.43	cytochrome P450 81E8-like [Cucurbita moschata]	[more]
KAG7034317.1	1.3e-244	84.63	Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022977458.1	1.8e-244	85.71	cytochrome P450 81E8-like [Cucurbita maxima]	[more]
XP_023543941.1	3.9e-244	84.63	cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo]	[more]
XP_004141996.2	9.4e-238	80.40	cytochrome P450 81E8 [Cucumis sativus] >KGN48565.1 hypothetical protein Csa_0035...	[more]

BLAST of Lag0013107 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	9.3e-164	57.68	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q6WNQ9	4.6e-163	56.40	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
P93147	7.4e-161	54.37	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ8	1.6e-160	55.86	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
Q6WNR0	1.5e-158	54.02	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Lag0013107 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCP2	2.9e-245	84.43	cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111452969 PE=3 SV=...	[more]
A0A6J1IQ18	8.5e-245	85.71	cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111477798 PE=3 SV=1	[more]
A0A0A0KFV2	4.5e-238	80.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G492250 PE=3 SV=1	[more]
A0A1S3B0W6	1.4e-231	79.16	cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103484812 PE=3 SV=1	[more]
A0A5D3CLS1	7.7e-230	78.76	Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Lag0013107 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37370.1	3.1e-162	57.64	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37340.1	6.8e-162	57.03	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT3G28740.1	5.4e-159	56.35	Cytochrome P450 superfamily protein	[more]
AT4G37360.1	2.7e-158	56.56	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT5G36220.1	1.5e-156	55.31	cytochrome p450 81d1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 144..164
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 17..467 e-value: 2.8E-18 score: 63.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 510..529
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 563..650
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 8..498
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 8..498
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 563..650
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 311..337 score: 36.72 coord: 291..308 score: 30.97 coord: 354..372 score: 36.1 coord: 439..462 score: 32.64 coord: 181..199 score: 22.12 coord: 395..419 score: 36.66 coord: 86..107 score: 19.12 coord: 429..439 score: 55.07 coord: 62..81 score: 42.64
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 355..366 score: 39.18 coord: 302..319 score: 37.41 coord: 430..439 score: 58.53
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 561..639 e-value: 1.6E-16 score: 60.1 coord: 35..481 e-value: 4.5E-97 score: 325.7
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 552..646 e-value: 1.1E-19 score: 72.3 coord: 20..491 e-value: 4.7E-127 score: 426.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 559..646
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 35..493
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 432..441
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 591..600

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0013107.1	Lag0013107.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen