Lag0012996 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0012996
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Transposon TX1 uncharacterized 149 kDa protein
Location	chr1: 46473753 .. 46475798 (+)
RNA-Seq Expression	Lag0012996
Synteny	Lag0012996

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACTTTCTCCCAACCAACATTGATTGGAGTCGAATTAGTGAAGCTCAACGACCACATTACAAGTTCCTTTCAAGGATGATGAGGTATATATAGTTGTCCAATCTTTGGGATCGAGTAAATCTCCTGGTCTTGATGGTTTCACTGCTGAATTTTTTAAACACTTTGGAGTACTATTAACAAGATGTGATGGCCATAGTGCATGAGTTTTACAAAAGTGGGATTATTAATGTGGCCTTCAATGAAACTTATATTTGTCTTATTCCAAAAAGAGTGGATTCCAGATCGGTAATGATTATCGTCCAATTAGCCTGATTTCTTGTGCTTATAAGATTATTGCTTGTGTCTTATCGAATGATTAAAGCAAGTTATCTCATTACAATTGCTGAAAATCATATGGCTTTTGTGTCTAATCGTCAAATCCTGGATGTTGCATTGATTGCAAGCGAGCTGATTGATGATTGGAAAACCTCCAATAAGAAAGGTGTGGTTATAAAGCTGGATTTAGAAAAAGCTTTTGATAAAGTTGATTGGGATTTTTGGTTGCGGTTCTTCAAATTAAAGGTTTTGGAAATACATGGAGGAAGTGGATTCGTGGTTGTGTCTCTAGTGCAAACTATTCGATTATTATTAATGGGCGCCTAGAGGAAAATTATTCCCACAAGAGGTATACGTCAGGGTGATCCATTATCTCCTTTTCTTTTTATCTTGGTTTCTGACTGTTTAAGTCGACTATTGTCTTTAGTGCGCGGTTGGGTAAATTGCCACTCACCCTATTGGTACCTCTTCTCTACATGTGACTCATTTGCAATTTGCTGATGATACTTTATTATTCTCTACTTCTAATGCTTCAGCGGTGGATAATCTTTTGATATTATTAAAATCTTTGAGAAGGCATCTGGCTTGAACATTAATTTTGGTAAAAGTGAATTATTGGGGATTAATGTTGATGATCAGAATATGGGAGTTTTCACTTCAAAGTTTGGCTGTAGACTTGGAGATTGGCCAACAACTTATCTTGGCCTTCCTTTAGGTGGCAATTCGAAATTACCCTCATTTTGGCACCCATGATTGAACAAATTCAGAAAAAGCTTCATAGTTGGAAGTATTTGTACATTTCAAAAGGTGGTAGATATACTCTAATTCAAGATTCTTTATCCAGCATGTCCATTTATTATCTCTCTTTGTATAAATTACCTTCAAAGGTGGCTAAAGTTTTGGATAAAATTATTCGTGATTTCTTTTGGGAAGGATCAACCGGAAATGGAGGATTACATAATATCAAATGGGAGACAACTCAACTTCCTCATATTTTGGGACGACTAGGAATTGGGAATTTTCAGCTTCGAAATTCTGCATTTCTGGCTAAATGGATTTGGAGGTTTCTTCATGAACATGAGAGTCTTTGGCGGAAATTAATAACCGCTAAATATTATTCTAGAGATGGTTTTGTTGGTGGCCAAAGCCCATTATGGGGGATCAACGAAATCTCTTTGGAAACACATTTGTAGCACCATTGATATTGTTGGTAGTCGTATTCAGAGGAGGCTTGGAAATGGGACTCACACTTCTTTTTGGAATGATTTGTGGCTTGCTGGTATATCTATCAGATCCTCTTATCCTAGACTTTATCGTTTATCATCTAGAACTACGGCAACGGTTGCTCAAATCTAGAATTCAGAATCTAAAGCTTGGGATTTAGGGCTTCGTCGAAATCTCAATGAGGAGGAAATTCAAGAATGGGCTAGTCTCTCACATCTCCTGTCATCAGTCATTTTCAGGAATACACAAGATTCGTGGTATTGGCATCTTGATCCCTCTCATTCATTTACAGTTCGGTCTTTGATGGAAGATTTAGTTCATTCAAAGGGTCCGACCAGGAACAATCTCTTTTCAATGATATGGAAGGATTGTTACCCAGAAAAGATTAAAATTTTCTTATGGGAGCTTAGTCATGGTGGAATTAATACAGCAGACAACTTCAAAGAAAATGCCTCATTTTCTTCTTTCTCCCTCGTGGTGTGTTATGTGTTGTGCAAGTGCTGA

mRNA sequence

ATGCACTTTCTCCCAACCAACATTGATTGGAGTCGAATTAGTGAAGCTCAACGACCACATTACAAGTTCCTTTCAAGGATGATGAGATTATTGCTTGTGTCTTATCGAATGATTAAAGCAAGTTATCTCATTACAATTGCTGAAAATCATATGGCTTTTGTGTCTAATCGTCAAATCCTGGATGTTGCATTGATTGCAAGCGAGCTGATTGATGATTGGAAAACCTCCAATAAGAAAGGTGTGGTTATAAAGCTGGATTTAGAAAAAGCTTTTGATAAAGTTGATTGGGATTTTTGGTTGCGGTTCTTCAAATTAAAGACTTGGAGATTGGCCAACAACTTATCTTGGCCTTCCTTTAGGTGGCAATTCGAAATTACCCTCATTTTGGCACCCATGATTGAACAAATTCAGAAAAAGCTTCATAGTTGGAAGTATTTGTACATTTCAAAAGGTGGTAGATATACTCTAATTCAAGATTCTTTATCCAGCATGTCCATTTATTATCTCTCTTTGTATAAATTACCTTCAAAGGTGGCTAAAGTTTTGGATAAAATTATTCGTGATTTCTTTTGGGAAGGATCAACCGGAAATGGAGGATTACATAATATCAAATGGGAGACAACTCAACTTCCTCATATTTTGGGACGACTAGGAATTGGGAATTTTCAGCTTCGAAATTCTGCATTTCTGGCTAAATGGATTTGGAGCACCATTGATATTGTTGGTAGTCGTATTCAGAGGAGGCTTGGAAATGGGACTCACACTTCTTTTTGGAATGATTTGTGGCTTGCTGAATCTAAAGCTTGGGATTTAGGGCTTCGTCGAAATCTCAATGAGGAGGAAATTCAAGAATGGGCTAGTCTCTCACATCTCCTGTCATCAGTCATTTTCAGGAATACACAAGATTCGTGGTATTGGCATCTTGATCCCTCTCATTCATTTACAGTTCGGTCTTTGATGGAAGATTTAGTTCATTCAAAGGGTCCGACCAGGAACAATCTCTTTTCAATGATATGGAAGGATTGTTACCCAGAAAAGATTAAAATTTTCTTATGGGAGCTTAGTCATGGTGGAATTAATACAGCAGACAACTTCAAAGAAAATGCCTCATTTTCTTCTTTCTCCCTCGTGGTGTGTTATGTGTTGTGCAAGTGCTGA

Coding sequence (CDS)

ATGCACTTTCTCCCAACCAACATTGATTGGAGTCGAATTAGTGAAGCTCAACGACCACATTACAAGTTCCTTTCAAGGATGATGAGATTATTGCTTGTGTCTTATCGAATGATTAAAGCAAGTTATCTCATTACAATTGCTGAAAATCATATGGCTTTTGTGTCTAATCGTCAAATCCTGGATGTTGCATTGATTGCAAGCGAGCTGATTGATGATTGGAAAACCTCCAATAAGAAAGGTGTGGTTATAAAGCTGGATTTAGAAAAAGCTTTTGATAAAGTTGATTGGGATTTTTGGTTGCGGTTCTTCAAATTAAAGACTTGGAGATTGGCCAACAACTTATCTTGGCCTTCCTTTAGGTGGCAATTCGAAATTACCCTCATTTTGGCACCCATGATTGAACAAATTCAGAAAAAGCTTCATAGTTGGAAGTATTTGTACATTTCAAAAGGTGGTAGATATACTCTAATTCAAGATTCTTTATCCAGCATGTCCATTTATTATCTCTCTTTGTATAAATTACCTTCAAAGGTGGCTAAAGTTTTGGATAAAATTATTCGTGATTTCTTTTGGGAAGGATCAACCGGAAATGGAGGATTACATAATATCAAATGGGAGACAACTCAACTTCCTCATATTTTGGGACGACTAGGAATTGGGAATTTTCAGCTTCGAAATTCTGCATTTCTGGCTAAATGGATTTGGAGCACCATTGATATTGTTGGTAGTCGTATTCAGAGGAGGCTTGGAAATGGGACTCACACTTCTTTTTGGAATGATTTGTGGCTTGCTGAATCTAAAGCTTGGGATTTAGGGCTTCGTCGAAATCTCAATGAGGAGGAAATTCAAGAATGGGCTAGTCTCTCACATCTCCTGTCATCAGTCATTTTCAGGAATACACAAGATTCGTGGTATTGGCATCTTGATCCCTCTCATTCATTTACAGTTCGGTCTTTGATGGAAGATTTAGTTCATTCAAAGGGTCCGACCAGGAACAATCTCTTTTCAATGATATGGAAGGATTGTTACCCAGAAAAGATTAAAATTTTCTTATGGGAGCTTAGTCATGGTGGAATTAATACAGCAGACAACTTCAAAGAAAATGCCTCATTTTCTTCTTTCTCCCTCGTGGTGTGTTATGTGTTGTGCAAGTGCTGA

Protein sequence

MHFLPTNIDWSRISEAQRPHYKFLSRMMRLLLVSYRMIKASYLITIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFWLRFFKLKTWRLANNLSWPSFRWQFEITLILAPMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIWSTIDIVGSRIQRRLGNGTHTSFWNDLWLAESKAWDLGLRRNLNEEEIQEWASLSHLLSSVIFRNTQDSWYWHLDPSHSFTVRSLMEDLVHSKGPTRNNLFSMIWKDCYPEKIKIFLWELSHGGINTADNFKENASFSSFSLVVCYVLCKC

Homology

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BLAST of Lag0012996 vs. NCBI nr
Match: RVW56165.1 (Calcium-transporting ATPase 3, endoplasmic reticulum-type [Vitis vinifera])

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-42
Identity = 130/448 (29.02%), Postives = 181/448 (40.40%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFW----- 104
           TI+++  AFV  R ILD  LIA+E++D+ + S ++G+V K+D EKA+D VDW F      
Sbjct: 86  TISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQ 145

Query: 105 ----------------LRFFKLKTWRLANNLSW-------PSFRWQFEITLILAPM---- 164
                              F+ +   L     W       P +  Q  ++  L P+    
Sbjct: 146 RKGVQPEMEIVADVLSRMLFRAEETGLTGVFLWGGIGQECPCYSLQMTLSFSLKPLWNIF 205

Query: 165 ------------------------------IEQIQKKLHSWKYLYISKGGRYTLIQDSLS 224
                                         +E+I ++L  WK  Y+S GGR TLIQ  LS
Sbjct: 206 KTSRLSFWFSGKFLVFLWEGTKDNRLWDPVVERISRRLDGWKKAYLSLGGRITLIQSCLS 265

Query: 225 SMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNF 284
            +  Y+LSL+K+P+ +A  ++K+ R+F W G+      H ++WE    P  LG LG G  
Sbjct: 266 HIPSYFLSLFKIPASIASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGGLGFGKI 325

Query: 285 QLRNSAFLAKWIWSTIDIVGSRIQRRL-------------GNGTHTSFWNDLWLAESKA- 344
            LRN A L KW+W        R  R               G     SFW DLW       
Sbjct: 326 SLRNIALLGKWLW--------RFPRERSGLWNFPFCSPSGGQWGENSFWEDLWWGNQSLC 385

Query: 345 ----------------------------WDLGLRRNLNEEEIQEWASLSHLLSSVIFR-N 385
                                       W+L  RRNL + EI     L   LS+V F  +
Sbjct: 386 SQFADLYRVISVKNLTVSNVLGNSFPLDWNLNFRRNLTDSEIDLLQRLMSSLSTVRFSPS 445

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BLAST of Lag0012996 vs. NCBI nr
Match: CAN68153.1 (hypothetical protein VITISV_009302 [Vitis vinifera])

HSP 1 Score: 179.9 bits (455), Expect = 4.3e-41
Identity = 120/393 (30.53%), Postives = 177/393 (45.04%), Query Frame = 0


Query: 45   TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFWLRFFK 104
            TI     AFV  RQILDV LIA+E++D+ + S ++GVV K+D EKA+D V+ D       
Sbjct: 830  TIHSTQGAFVQGRQILDVVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVNLD------- 889

Query: 105  LKTWRLAN------NLSWPS---FRWQFEITLILA--------------PMIEQIQKKLH 164
             K+  +A+       L W S    R    + L L               P+IE+I ++L+
Sbjct: 890  -KSNIMASILIKIIFLDWLSCLIARLLVGLLLYLGLPLGGNPKACGFWDPVIERISRRLN 949

Query: 165  SWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLH 224
             W+  Y+S  GR TLIQ  L+ M  Y LSL+K+P+ +A  ++++ RDF W G   +   H
Sbjct: 950  GWQKTYLSFRGRITLIQSCLTHMPCYILSLFKIPALMAAKIERLQRDFLWSGIGEDKRDH 1009

Query: 225  NIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIWSTIDIVGSRIQRRLGNGTHTSFWNDL 284
             I W+    P   G LG G   LRN A L KW+W  +         R GN     FW DL
Sbjct: 1010 LISWDVVCKPKAKGGLGFGKISLRNLALLGKWLWRYL---------REGNEEIIRFWEDL 1069

Query: 285  WLAESK-----------------------------AWDLGLRRNLNEEEIQEWASLSHLL 344
            W                                  +W+    RNL++ EI++   L   L
Sbjct: 1070 WWRNQPLGSQYPRLFRVVLDKNVPISSILDSTRPFSWNFNFLRNLSDSEIEDLKGLMRSL 1129

Query: 345  SSV-IFRNTQDSWYWHLDPSHSFTVRSLMEDLVHSKGPTRNNLFSMIWKDCYPEKIKIFL 385
              + +     D+  W L PS  F+V+S    L    G  +      +WK   P K+K F+
Sbjct: 1130 DGLHLSPPVSDARSWPLSPSRLFSVKSFFLALSQFSGSLQVFPSKFVWKSQVPFKVKSFV 1189

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BLAST of Lag0012996 vs. NCBI nr
Match: RVW99862.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 167.9 bits (424), Expect = 1.7e-37
Identity = 125/425 (29.41%), Postives = 178/425 (41.88%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFWLRFFK 104
           TI+ +  AFV  R ILD  LIA+E++D+ + S ++G+V K+D EKA+D VDW F     +
Sbjct: 365 TISGSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQ 424

Query: 105 LK----TWR--LANNLSWPSFR---------W--------------QFEITLIL------ 164
            K     WR  +   LS  SF          W               F  TL+       
Sbjct: 425 RKGFNQKWRSWIRGCLSSSSFAILVNGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSLP 484

Query: 165 -----------APMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKV 224
                       P++E+I ++L  WK  Y+S GGR  LIQ  LS +  Y+LSL+K+P+ +
Sbjct: 485 LGGNPKTIGFWDPVVERISRRLDGWKKAYLSLGGRIMLIQSCLSHIPSYFLSLFKIPASI 544

Query: 225 AKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLR-NSAFLAKWI--- 284
           A  ++K+ R+F W G+      H ++WE    P  LG    G      ++  + +W    
Sbjct: 545 ASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGVSIYGTHPNGWDANMVVRWSHRC 604

Query: 285 -WSTIDIVGSR----IQRRLGNGTHTSFWNDLWLAESK---------------------- 344
            W  I  V       ++  +GNG    FW DLW                           
Sbjct: 605 PWKVIAQVFQEFSPFVRLVVGNGERIRFWEDLWWGNQSLCSQFADLYRVISVKNLTVSNV 664

Query: 345 -------AWDLGLRRNLNEEEIQEWASLSHLLSSVIFR-NTQDSWYWHLDPSHSFTVRSL 385
                  AW+L  R NL + EI     L   LSSV F  +  DS  W L  S  F+V+S 
Sbjct: 665 LGNSFPLAWNLNFRHNLIDSEIDLLQRLMSSLSSVRFSPSLADSRAWSLSSSGLFSVKSF 724

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BLAST of Lag0012996 vs. NCBI nr
Match: RVX17758.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-35
Identity = 91/292 (31.16%), Postives = 131/292 (44.86%), Query Frame = 0


Query: 131 PMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFF 190
           P++E+I ++L  WK  Y+S GGR TLIQ  LS +  Y+LSL+K+P  +A  ++K+ RDF 
Sbjct: 63  PVVERISRRLDGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIPVSIASKIEKMQRDFL 122

Query: 191 WEGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIWS--------TIDIVG 250
           W G+      H I+WE    P  +G LG G   +RNSA L KW+W               
Sbjct: 123 WSGAGEGKKDHLIRWEVVSRPREMGGLGFGKTSMRNSALLGKWLWRFPRERSGLWHKEFS 182

Query: 251 SRIQRRLGNGTHTSFWNDLWLAESK-----------------------------AWDLGL 310
             ++  +GNG    FW DLW                                  +W+   
Sbjct: 183 PFVRLVVGNGERIRFWEDLWWGNQTLCSQFADLYRVISMKNLTVSNVLGNSLPLSWNFNF 242

Query: 311 RRNLNEEEIQEWASLSHLLSSVIFR-NTQDSWYWHLDPSHSFTVRSLMEDLVHSKGPTRN 370
           RRNL + EI     L   L+SV+   ++ DS  W L  S SF+V+S    L     P   
Sbjct: 243 RRNLTDSEIDLLQRLMSSLNSVLLSPSSSDSRVWSLSSSSSFSVKSFFYALSKVSNPLMF 302

Query: 371 NLFSMIWKDCYPEKIKIFLWELSHGGINTADNFKENASFSSFSLVVCYVLCK 385
                +W    P K+K   W ++HG +NT D  +    + +     C +LCK
Sbjct: 303 LPAKFLWSSKVPSKVKALAWLVAHGKVNTNDKLQLRRPYKALYPQWC-ILCK 353

[Close]

BLAST of Lag0012996 vs. NCBI nr
Match: RVX22450.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 161.8 bits (408), Expect = 1.2e-35
Identity = 117/445 (26.29%), Postives = 170/445 (38.20%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDF------ 104
           TI  +  AFV  RQILD  LIA+E++D+ + S ++GVV K+D +K +D VDW F      
Sbjct: 46  TIFGSQGAFVEGRQILDAVLIANEVVDEKRRSGEEGVVFKIDFKKTYDHVDWSFLDHVLQ 105

Query: 105 ----------WLR----------------------------------------------- 164
                     W+R                                               
Sbjct: 106 RKGFSQKWRSWMRGCLSSSSFAILVNGNAKGWVKASRAEETGITEGFLVGRDRTKVSLLQ 165

Query: 165 ------FFKLKTWRLANNL--------SWPSFRWQFEITLILA--------------PMI 224
                 FF   +  +  NL             +   E + I                P++
Sbjct: 166 FADDTIFFSKASLDILQNLKIILLVFGQVSGLKINLEKSTISGLPLGGNPKTIGFWDPVV 225

Query: 225 EQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFWEG 284
           E+I ++L  WK  Y+S GGR TLIQ  L  +  Y+L+L+K+P  +A  ++K+ RDF W G
Sbjct: 226 ERISRRLDGWKKAYLSLGGRITLIQSCLFHIPSYFLTLFKIPVYIASKIEKMQRDFLWSG 285

Query: 285 STGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIW-----------STIDIVG 344
           +      H I+WE    P  +G LG G   +RNSA L KW+W             I  V 
Sbjct: 286 AREGKKDHLIRWEVVNRPKEMGGLGFGKTSMRNSALLGKWLWRFPRKMSGLWHKVIARVL 345

Query: 345 SRIQRRLGNGTHTSFWNDLWLAESK-----------------------------AWDLGL 358
             ++  + NG    FW DLW                                  +W+   
Sbjct: 346 PFVRLVVSNGERIRFWEDLWWGNQTLYSQFADLYRVISVKNLTVSNVLGNSLPLSWNFNF 405

[Close]

BLAST of Lag0012996 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.6e-14
Identity = 65/306 (21.24%), Postives = 111/306 (36.27%), Query Frame = 0


Query: 132 MIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFW 191
           ++E++  ++  W+   +S  GR TL +  LSSM ++ +S   LP  +   LD++ R F W
Sbjct: 16  ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 192 EGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAK------------------- 251
             +      H +KW     P   G LG+   +  N A ++K                   
Sbjct: 76  GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 252 -----------WI---------WSTI-----DIVGSRIQRRLGNGTHTSFWNDLWLA--- 311
                      W+         W +I     D+V   +    G+G    FW D W++   
Sbjct: 136 KYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKP 195

Query: 312 -----------------------ESKAWDLGL--RRNLNEEEIQEWASLSHLLSSVIFRN 364
                                    + WD         N   ++  A +  L++      
Sbjct: 196 LLELDNGERPTDCDTVVAKDLWIPGRGWDFAKIDPYTTNNTRLELRAVVLDLVT-----G 255

[Close]

BLAST of Lag0012996 vs. ExPASy TrEMBL
Match: A0A438F829 (Calcium-transporting ATPase 3, endoplasmic reticulum-type OS=Vitis vinifera OX=29760 GN=ECA3_7 PE=4 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 6.4e-43
Identity = 130/448 (29.02%), Postives = 181/448 (40.40%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFW----- 104
           TI+++  AFV  R ILD  LIA+E++D+ + S ++G+V K+D EKA+D VDW F      
Sbjct: 86  TISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQ 145

Query: 105 ----------------LRFFKLKTWRLANNLSW-------PSFRWQFEITLILAPM---- 164
                              F+ +   L     W       P +  Q  ++  L P+    
Sbjct: 146 RKGVQPEMEIVADVLSRMLFRAEETGLTGVFLWGGIGQECPCYSLQMTLSFSLKPLWNIF 205

Query: 165 ------------------------------IEQIQKKLHSWKYLYISKGGRYTLIQDSLS 224
                                         +E+I ++L  WK  Y+S GGR TLIQ  LS
Sbjct: 206 KTSRLSFWFSGKFLVFLWEGTKDNRLWDPVVERISRRLDGWKKAYLSLGGRITLIQSCLS 265

Query: 225 SMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNF 284
            +  Y+LSL+K+P+ +A  ++K+ R+F W G+      H ++WE    P  LG LG G  
Sbjct: 266 HIPSYFLSLFKIPASIASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGGLGFGKI 325

Query: 285 QLRNSAFLAKWIWSTIDIVGSRIQRRL-------------GNGTHTSFWNDLWLAESKA- 344
            LRN A L KW+W        R  R               G     SFW DLW       
Sbjct: 326 SLRNIALLGKWLW--------RFPRERSGLWNFPFCSPSGGQWGENSFWEDLWWGNQSLC 385

Query: 345 ----------------------------WDLGLRRNLNEEEIQEWASLSHLLSSVIFR-N 385
                                       W+L  RRNL + EI     L   LS+V F  +
Sbjct: 386 SQFADLYRVISVKNLTVSNVLGNSFPLDWNLNFRRNLTDSEIDLLQRLMSSLSTVRFSPS 445

[Close]

BLAST of Lag0012996 vs. ExPASy TrEMBL
Match: A5BNY2 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_009302 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.1e-41
Identity = 120/393 (30.53%), Postives = 177/393 (45.04%), Query Frame = 0


Query: 45   TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFWLRFFK 104
            TI     AFV  RQILDV LIA+E++D+ + S ++GVV K+D EKA+D V+ D       
Sbjct: 830  TIHSTQGAFVQGRQILDVVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVNLD------- 889

Query: 105  LKTWRLAN------NLSWPS---FRWQFEITLILA--------------PMIEQIQKKLH 164
             K+  +A+       L W S    R    + L L               P+IE+I ++L+
Sbjct: 890  -KSNIMASILIKIIFLDWLSCLIARLLVGLLLYLGLPLGGNPKACGFWDPVIERISRRLN 949

Query: 165  SWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLH 224
             W+  Y+S  GR TLIQ  L+ M  Y LSL+K+P+ +A  ++++ RDF W G   +   H
Sbjct: 950  GWQKTYLSFRGRITLIQSCLTHMPCYILSLFKIPALMAAKIERLQRDFLWSGIGEDKRDH 1009

Query: 225  NIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIWSTIDIVGSRIQRRLGNGTHTSFWNDL 284
             I W+    P   G LG G   LRN A L KW+W  +         R GN     FW DL
Sbjct: 1010 LISWDVVCKPKAKGGLGFGKISLRNLALLGKWLWRYL---------REGNEEIIRFWEDL 1069

Query: 285  WLAESK-----------------------------AWDLGLRRNLNEEEIQEWASLSHLL 344
            W                                  +W+    RNL++ EI++   L   L
Sbjct: 1070 WWRNQPLGSQYPRLFRVVLDKNVPISSILDSTRPFSWNFNFLRNLSDSEIEDLKGLMRSL 1129

Query: 345  SSV-IFRNTQDSWYWHLDPSHSFTVRSLMEDLVHSKGPTRNNLFSMIWKDCYPEKIKIFL 385
              + +     D+  W L PS  F+V+S    L    G  +      +WK   P K+K F+
Sbjct: 1130 DGLHLSPPVSDARSWPLSPSRLFSVKSFFLALSQFSGSLQVFPSKFVWKSQVPFKVKSFV 1189

[Close]

BLAST of Lag0012996 vs. ExPASy TrEMBL
Match: A0A438IT11 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_617 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 8.1e-38
Identity = 125/425 (29.41%), Postives = 178/425 (41.88%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDFWLRFFK 104
           TI+ +  AFV  R ILD  LIA+E++D+ + S ++G+V K+D EKA+D VDW F     +
Sbjct: 365 TISGSQGAFVEGRHILDAVLIANEVVDEKRRSGEEGIVFKIDFEKAYDHVDWGFLDHVLQ 424

Query: 105 LK----TWR--LANNLSWPSFR---------W--------------QFEITLIL------ 164
            K     WR  +   LS  SF          W               F  TL+       
Sbjct: 425 RKGFNQKWRSWIRGCLSSSSFAILVNGNAKGWVKASRGLRQGDPLSPFLFTLVADVLSLP 484

Query: 165 -----------APMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKV 224
                       P++E+I ++L  WK  Y+S GGR  LIQ  LS +  Y+LSL+K+P+ +
Sbjct: 485 LGGNPKTIGFWDPVVERISRRLDGWKKAYLSLGGRIMLIQSCLSHIPSYFLSLFKIPASI 544

Query: 225 AKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLR-NSAFLAKWI--- 284
           A  ++K+ R+F W G+      H ++WE    P  LG    G      ++  + +W    
Sbjct: 545 ASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGVSIYGTHPNGWDANMVVRWSHRC 604

Query: 285 -WSTIDIVGSR----IQRRLGNGTHTSFWNDLWLAESK---------------------- 344
            W  I  V       ++  +GNG    FW DLW                           
Sbjct: 605 PWKVIAQVFQEFSPFVRLVVGNGERIRFWEDLWWGNQSLCSQFADLYRVISVKNLTVSNV 664

Query: 345 -------AWDLGLRRNLNEEEIQEWASLSHLLSSVIFR-NTQDSWYWHLDPSHSFTVRSL 385
                  AW+L  R NL + EI     L   LSSV F  +  DS  W L  S  F+V+S 
Sbjct: 665 LGNSFPLAWNLNFRHNLIDSEIDLLQRLMSSLSSVRFSPSLADSRAWSLSSSGLFSVKSF 724

[Close]

BLAST of Lag0012996 vs. ExPASy TrEMBL
Match: A0A438KMM1 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_114 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.8e-36
Identity = 117/445 (26.29%), Postives = 170/445 (38.20%), Query Frame = 0


Query: 45  TIAENHMAFVSNRQILDVALIASELIDDWKTSNKKGVVIKLDLEKAFDKVDWDF------ 104
           TI  +  AFV  RQILD  LIA+E++D+ + S ++GVV K+D +K +D VDW F      
Sbjct: 46  TIFGSQGAFVEGRQILDAVLIANEVVDEKRRSGEEGVVFKIDFKKTYDHVDWSFLDHVLQ 105

Query: 105 ----------WLR----------------------------------------------- 164
                     W+R                                               
Sbjct: 106 RKGFSQKWRSWMRGCLSSSSFAILVNGNAKGWVKASRAEETGITEGFLVGRDRTKVSLLQ 165

Query: 165 ------FFKLKTWRLANNL--------SWPSFRWQFEITLILA--------------PMI 224
                 FF   +  +  NL             +   E + I                P++
Sbjct: 166 FADDTIFFSKASLDILQNLKIILLVFGQVSGLKINLEKSTISGLPLGGNPKTIGFWDPVV 225

Query: 225 EQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFFWEG 284
           E+I ++L  WK  Y+S GGR TLIQ  L  +  Y+L+L+K+P  +A  ++K+ RDF W G
Sbjct: 226 ERISRRLDGWKKAYLSLGGRITLIQSCLFHIPSYFLTLFKIPVYIASKIEKMQRDFLWSG 285

Query: 285 STGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIW-----------STIDIVG 344
           +      H I+WE    P  +G LG G   +RNSA L KW+W             I  V 
Sbjct: 286 AREGKKDHLIRWEVVNRPKEMGGLGFGKTSMRNSALLGKWLWRFPRKMSGLWHKVIARVL 345

Query: 345 SRIQRRLGNGTHTSFWNDLWLAESK-----------------------------AWDLGL 358
             ++  + NG    FW DLW                                  +W+   
Sbjct: 346 PFVRLVVSNGERIRFWEDLWWGNQTLYSQFADLYRVISVKNLTVSNVLGNSLPLSWNFNF 405

[Close]

BLAST of Lag0012996 vs. ExPASy TrEMBL
Match: A0A438K969 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_73 PE=4 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 5.8e-36
Identity = 91/292 (31.16%), Postives = 131/292 (44.86%), Query Frame = 0


Query: 131 PMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFF 190
           P++E+I ++L  WK  Y+S GGR TLIQ  LS +  Y+LSL+K+P  +A  ++K+ RDF 
Sbjct: 63  PVVERISRRLDGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIPVSIASKIEKMQRDFL 122

Query: 191 WEGSTGNGGLHNIKWETTQLPHILGRLGIGNFQLRNSAFLAKWIWS--------TIDIVG 250
           W G+      H I+WE    P  +G LG G   +RNSA L KW+W               
Sbjct: 123 WSGAGEGKKDHLIRWEVVSRPREMGGLGFGKTSMRNSALLGKWLWRFPRERSGLWHKEFS 182

Query: 251 SRIQRRLGNGTHTSFWNDLWLAESK-----------------------------AWDLGL 310
             ++  +GNG    FW DLW                                  +W+   
Sbjct: 183 PFVRLVVGNGERIRFWEDLWWGNQTLCSQFADLYRVISMKNLTVSNVLGNSLPLSWNFNF 242

Query: 311 RRNLNEEEIQEWASLSHLLSSVIFR-NTQDSWYWHLDPSHSFTVRSLMEDLVHSKGPTRN 370
           RRNL + EI     L   L+SV+   ++ DS  W L  S SF+V+S    L     P   
Sbjct: 243 RRNLTDSEIDLLQRLMSSLNSVLLSPSSSDSRVWSLSSSSSFSVKSFFYALSKVSNPLMF 302

Query: 371 NLFSMIWKDCYPEKIKIFLWELSHGGINTADNFKENASFSSFSLVVCYVLCK 385
                +W    P K+K   W ++HG +NT D  +    + +     C +LCK
Sbjct: 303 LPAKFLWSSKVPSKVKALAWLVAHGKVNTNDKLQLRRPYKALYPQWC-ILCK 353

[Close]

BLAST of Lag0012996 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 79.3 bits (194), Expect = 7.3e-15
Identity = 41/145 (28.28%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 131 PMIEQIQKKLHSWKYLYISKGGRYTLIQDSLSSMSIYYLSLYKLPSKVAKVLDKIIRDFF 190
           P++E+I+ ++  W   ++S  GR  LI   + S++ +++S ++LPS   K +D I   F 
Sbjct: 44  PLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFL 103

Query: 191 WEGSTGNGGLHNIKWETTQLPHILGRLGI--------GNF-QLRNSAFLAKWIWSTI--- 250
           W G   N     + W     P   G LGI        G+F  +  +  L  W+W  I   
Sbjct: 104 WSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKGSFWSISGNTTLGSWMWKKILKH 163

Query: 251 -DIVGSRIQRRLGNGTHTSFWNDLW 263
             +    ++  + NG++TSFW D W
Sbjct: 164 RALASGFVKHDIHNGSNTSFWFDNW 188

[Close]

BLAST of Lag0012996 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 42.4 bits (98), Expect = 9.9e-04
Identity = 18/73 (24.66%), Postives = 33/73 (45.21%), Query Frame = 0


Query: 163 SMSIYYLSLYKLPSKVAKVLDKIIRDFFWEGSTGNGGLHNIKWETTQLPHILGRLGIGNF 222
           ++  Y ++ + LP  V K +  ++ DF+W       G+H   W+        G +G  + 
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 223 QLRNSAFLAKWIW 236
           +  N A L K +W
Sbjct: 62  EAFNLALLGKQMW 74

The following BLAST results are available for this feature:

BLAST of Lag0012996 vs. NCBI nr


Match Name	E-value	Identity	Description
RVW56165.1	1.3e-42	29.02	Calcium-transporting ATPase 3, endoplasmic reticulum-type [Vitis vinifera]	[more]
CAN68153.1	4.3e-41	30.53	hypothetical protein VITISV_009302 [Vitis vinifera]	[more]
RVW99862.1	1.7e-37	29.41	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]
RVX17758.1	1.2e-35	31.16	putative ribonuclease H protein [Vitis vinifera]	[more]
RVX22450.1	1.2e-35	26.29	putative ribonuclease H protein [Vitis vinifera]	[more]

BLAST of Lag0012996 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	4.6e-14	21.24	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of Lag0012996 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A438F829	6.4e-43	29.02	Calcium-transporting ATPase 3, endoplasmic reticulum-type OS=Vitis vinifera OX=2...	[more]
A5BNY2	2.1e-41	30.53	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_009302 PE=4 SV=1	[more]
A0A438IT11	8.1e-38	29.41	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]
A0A438KMM1	5.8e-36	26.29	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_114 P...	[more]
A0A438K969	5.8e-36	31.16	Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDp000001_73 PE...	[more]

BLAST of Lag0012996 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24255.1	7.3e-15	28.28	RNA-directed DNA polymerase (reverse transcriptase)-related family protein	[more]
AT4G29090.1	9.9e-04	24.66	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33116:SF33	OS01G0885550 PROTEIN	coord: 45..104 coord: 130..362
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 45..104 coord: 130..362

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0012996.1	Lag0012996.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006807	nitrogen compound metabolic process
biological_process	GO:0071704	organic substance metabolic process
biological_process	GO:0044238	primary metabolic process
biological_process	GO:0050789	regulation of biological process
biological_process	GO:0050896	response to stimulus
molecular_function	GO:0003824	catalytic activity