Lag0012949 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012949
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionalkane hydroxylase MAH1-like
Locationchr1: 46019265 .. 46020155 (+)
RNA-Seq ExpressionLag0012949
SyntenyLag0012949
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCGCTCGACCCTTTTCTTACCATTTTTCTCGCCTCATTGCCCTTCATTTTCATCCTTGTCTTTCTCTCTCTCAACCTGAATCCCTATGGGCTTCCATGGAATTGGCCCATTTTCGGAATGATTCCCACTATTTTCCACCATATCCACCGAATCCACGATCGTTTTACCGAAATCATGCACGTAACTTCCTGCACTTTCTTCTTCAAAGGCATTTGGTTCACCAATACAGGCTTCTTGATGACCGTCGATCCCGCCAACATTCACCACATCATGAGCACCAACTTCCAAAAATACCCAAAAGGCCCTGATTTCAAGTACATTTTCGACGTTTTTGGAGATGGGATTTTCAACTCCGACTCCGATTCCTGGAAGACCCAACGCAGAATCGCTCAGTCTCTGATAGTCCACGAGAAGTTTCTTCGGTTCATGGCCGGAGCCGCCAGGGAGAAGGTGGAGAAAGGGCTGATGCCGGTGCTCGACTTCTTCTGTGAAAATGGAAGCGTGGTTGATTTGAAAGATTTGTTTTTGAGATTGGCGTTTGATTCTACTTGTATGATGGTTACTGGGTTCGATCTCAACAGTCTGTCTCTTGAGTTTCCTGAAATTCCCTTCTCTAAGGCCATGGATGACGTTCAAGAAGTGCTTTTTCTTCGTCATTTCTATCCTAGAATGTATTGGGAGTTGCTGAAGAAGCTGGACATTGGAGAGGCCAAGAGGATGAGGAAGGCTACCGATACGATAGATCAAGTCATTGCCAACTTGATGGCCGTGAAAAGAGAGCGTCTGAAACGAGCTCGAGAGTTGAATGAAGACTTACAACACGGTGATGCTGATTTGATCACAGGGTATATATCCTTTAATTTTCACTTTAACACAAAAGTTTAA

mRNA sequence

ATGGCGTCGCTCGACCCTTTTCTTACCATTTTTCTCGCCTCATTGCCCTTCATTTTCATCCTTGTCTTTCTCTCTCTCAACCTGAATCCCTATGGGCTTCCATGGAATTGGCCCATTTTCGGAATGATTCCCACTATTTTCCACCATATCCACCGAATCCACGATCGTTTTACCGAAATCATGCACGTAACTTCCTGCACTTTCTTCTTCAAAGGCATTTGGTTCACCAATACAGGCTTCTTGATGACCGTCGATCCCGCCAACATTCACCACATCATGAGCACCAACTTCCAAAAATACCCAAAAGGCCCTGATTTCAAGTACATTTTCGACGTTTTTGGAGATGGGATTTTCAACTCCGACTCCGATTCCTGGAAGACCCAACGCAGAATCGCTCAGTCTCTGATAGTCCACGAGAAGTTTCTTCGGTTCATGGCCGGAGCCGCCAGGGAGAAGGTGGAGAAAGGGCTGATGCCGGTGCTCGACTTCTTCTGTGAAAATGGAAGCGTGGTTGATTTGAAAGATTTGTTTTTGAGATTGGCGTTTGATTCTACTTGTATGATGGTTACTGGGTTCGATCTCAACAGTCTGTCTCTTGAGTTTCCTGAAATTCCCTTCTCTAAGGCCATGGATGACGTTCAAGAAGTGCTTTTTCTTCGTCATTTCTATCCTAGAATGTATTGGGAGTTGCTGAAGAAGCTGGACATTGGAGAGGCCAAGAGGATGAGGAAGGCTACCGATACGATAGATCAAGTCATTGCCAACTTGATGGCCGTGAAAAGAGAGCGTCTGAAACGAGCTCGAGAGTTGAATGAAGACTTACAACACGGTGATGCTGATTTGATCACAGGGTATATATCCTTTAATTTTCACTTTAACACAAAAGTTTAA

Coding sequence (CDS)

ATGGCGTCGCTCGACCCTTTTCTTACCATTTTTCTCGCCTCATTGCCCTTCATTTTCATCCTTGTCTTTCTCTCTCTCAACCTGAATCCCTATGGGCTTCCATGGAATTGGCCCATTTTCGGAATGATTCCCACTATTTTCCACCATATCCACCGAATCCACGATCGTTTTACCGAAATCATGCACGTAACTTCCTGCACTTTCTTCTTCAAAGGCATTTGGTTCACCAATACAGGCTTCTTGATGACCGTCGATCCCGCCAACATTCACCACATCATGAGCACCAACTTCCAAAAATACCCAAAAGGCCCTGATTTCAAGTACATTTTCGACGTTTTTGGAGATGGGATTTTCAACTCCGACTCCGATTCCTGGAAGACCCAACGCAGAATCGCTCAGTCTCTGATAGTCCACGAGAAGTTTCTTCGGTTCATGGCCGGAGCCGCCAGGGAGAAGGTGGAGAAAGGGCTGATGCCGGTGCTCGACTTCTTCTGTGAAAATGGAAGCGTGGTTGATTTGAAAGATTTGTTTTTGAGATTGGCGTTTGATTCTACTTGTATGATGGTTACTGGGTTCGATCTCAACAGTCTGTCTCTTGAGTTTCCTGAAATTCCCTTCTCTAAGGCCATGGATGACGTTCAAGAAGTGCTTTTTCTTCGTCATTTCTATCCTAGAATGTATTGGGAGTTGCTGAAGAAGCTGGACATTGGAGAGGCCAAGAGGATGAGGAAGGCTACCGATACGATAGATCAAGTCATTGCCAACTTGATGGCCGTGAAAAGAGAGCGTCTGAAACGAGCTCGAGAGTTGAATGAAGACTTACAACACGGTGATGCTGATTTGATCACAGGGTATATATCCTTTAATTTTCACTTTAACACAAAAGTTTAA

Protein sequence

MASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYISFNFHFNTKV
Homology
BLAST of Lag0012949 vs. NCBI nr
Match: XP_038882847.1 (alkane hydroxylase MAH1-like [Benincasa hispida])

HSP 1 Score: 471.1 bits (1211), Expect = 7.1e-129
Identity = 230/282 (81.56%), Postives = 250/282 (88.65%), Query Frame = 0

Query: 7   FLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSC 66
           FL IFL SLPFIFIL  L L  NP+GLPWNWPI GMIPT+ HHIHR+HDR TE++ +TSC
Sbjct: 7   FLPIFLISLPFIFILFSLFLKWNPHGLPWNWPILGMIPTVIHHIHRVHDRITEVLQITSC 66

Query: 67  TFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWK 126
           TF+FKGIWFTNTGFLMTVDP+NIHHIMSTNFQ YPKGP+FKYIFDV GDGIFNSDSDSWK
Sbjct: 67  TFYFKGIWFTNTGFLMTVDPSNIHHIMSTNFQNYPKGPEFKYIFDVLGDGIFNSDSDSWK 126

Query: 127 TQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGS-VVDLKDLFLRLAFDST 186
            QR+IAQSLIVHEKFL FMA AA EKVEKGL+P+LD+FCEN S VVDLKDLFLRLAFDST
Sbjct: 127 NQRKIAQSLIVHEKFLEFMARAAMEKVEKGLVPILDYFCENKSLVVDLKDLFLRLAFDST 186

Query: 187 CMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKA 246
           CMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP++YWELLKKL IGE +RM+KA
Sbjct: 187 CMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKLYWELLKKLGIGETRRMKKA 246

Query: 247 TDTIDQVIANLMAVKRERLKRARELNE-DLQHGDADLITGYI 287
            DTID+VIANLMAVKRERL+R REL E  LQ   ADLIT Y+
Sbjct: 247 CDTIDEVIANLMAVKRERLQRDRELKELQLQDRGADLITWYM 288

BLAST of Lag0012949 vs. NCBI nr
Match: XP_022963151.1 (alkane hydroxylase MAH1-like [Cucurbita moschata])

HSP 1 Score: 469.9 bits (1208), Expect = 1.6e-128
Identity = 227/285 (79.65%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHKRNPHGIPWNWPIVGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVHEKFL+FMAGAAREKVEKGL+PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHEKFLKFMAGAAREKVEKGLIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP+MYWEL+KKLD+GEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKMYWELIKKLDVGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKA +TID VIANL+AVK ERL RARE+ E+L  G ADLIT Y+
Sbjct: 243 MRKAAETIDHVIANLLAVKTERLNRAREMKEELHDGGADLITWYM 286

BLAST of Lag0012949 vs. NCBI nr
Match: KAG6595090.1 (Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 226/285 (79.30%), Postives = 253/285 (88.77%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHKRNPHGIPWNWPIVGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVH+KFL+FMAGAAREKVEKG++PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHDKFLKFMAGAAREKVEKGVIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP+MYWEL+KKLD+GEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKMYWELMKKLDVGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKAT+TID VIANL+AVK ERL RARE+ E+L  G ADLIT Y+
Sbjct: 243 MRKATETIDHVIANLLAVKTERLNRAREMKEELHDGGADLITWYM 286

BLAST of Lag0012949 vs. NCBI nr
Match: XP_023004057.1 (alkane hydroxylase MAH1-like [Cucurbita maxima])

HSP 1 Score: 468.4 bits (1204), Expect = 4.6e-128
Identity = 227/285 (79.65%), Postives = 251/285 (88.07%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHKRNPHGIPWNWPILGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVHEKFL+FMAGAAREKVEKGL+PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHEKFLKFMAGAAREKVEKGLIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP+MYWEL+KKLDIGEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKMYWELMKKLDIGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKA +TID VIANL+AVK ERL R RE+ E+L  G ADLIT Y+
Sbjct: 243 MRKAAETIDHVIANLLAVKTERLNRDREIKEELHDGGADLITWYM 286

BLAST of Lag0012949 vs. NCBI nr
Match: XP_023518531.1 (alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 225/285 (78.95%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHRRNPHGIPWNWPIVGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVHEKFL+FMAGAAREKVEKG++PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHEKFLKFMAGAAREKVEKGVIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLF+RH YP+MYWEL+KKLD+GEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFVRHLYPKMYWELMKKLDVGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKA +TID VIANL+AVK ERL RARE+ E+L  G ADLIT Y+
Sbjct: 243 MRKAAETIDHVIANLLAVKTERLNRAREMKEELPDGGADLITWYM 286

BLAST of Lag0012949 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 3.6e-51
Identity = 100/259 (38.61%), Postives = 158/259 (61.00%), Query Frame = 0

Query: 17  FIFILV----FLSLNLNPYGLP--WNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSCTFFF 76
           FIF LV    +  L   P G P   NWP   M+P + H I RI+D   E++  T+ TF+F
Sbjct: 10  FIFFLVCLFTYFFLQKKPQGQPILKNWPFLRMLPGMLHQIPRIYDWTVEVLEATNLTFYF 69

Query: 77  KGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWKTQRR 136
           KG W + T  L T DP NIHHI+S+NF  YPKGP+FK IFDV G+GI   D + W+  R+
Sbjct: 70  KGPWLSGTDMLFTADPRNIHHILSSNFGNYPKGPEFKKIFDVLGEGILTVDFELWEEMRK 129

Query: 137 IAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTCMMVT 196
              +L  ++ F+     + + K+++GL+P LD   +   +++L+D+F R  FD++ +++T
Sbjct: 130 SNHALFHNQDFIELSVSSNKSKLKEGLVPFLDNAAQKNIIIELQDVFQRFMFDTSSILMT 189

Query: 197 GFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKATDTID 256
           G+D  SLS+E  E+ F +A D  +E ++ RHF P + W L   + IG  ++MR A  T++
Sbjct: 190 GYDPMSLSIEMLEVEFGEAADIGEEAIYYRHFKPVILWRLQNWIGIGLERKMRTALATVN 249

Query: 257 QVIANLMAVKR-ERLKRAR 269
           ++ A +++ +R E + RA+
Sbjct: 250 RMFAKIISSRRKEEISRAK 268

BLAST of Lag0012949 vs. ExPASy Swiss-Prot
Match: A0A2H5AJ00 (Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.3e-45
Identity = 95/275 (34.55%), Postives = 156/275 (56.73%), Query Frame = 0

Query: 18  IFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSCTFFFKGIWFTN 77
           +F L+  + + +  GLP+NWPIFGM+P I  + ++ +D  T  +     TF FKG W  +
Sbjct: 27  VFSLLRSARSSSKGGLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGWTFIFKGPWLLD 86

Query: 78  TGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWKTQRRIAQSLIV 137
             ++ T DP+NI+H+ + NF+ YPKG +   +FD+FG+ IFN+D D W   R++AQ+++ 
Sbjct: 87  MDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWHDHRKMAQTILW 146

Query: 138 HEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTCMMVTGFDLNSL 197
              +    A   R K++  L+P+LD      + VDL+D+ LR  FD++C  V   D  SL
Sbjct: 147 DGNYRTMQATFIRNKMDNALIPILDSAACKRNPVDLQDVLLRFTFDTSCFSVLAADPESL 206

Query: 198 SLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKATDTIDQVIANLM 257
           ++EFP +PFSKA D   +    RH  PR+ W+L +  ++G  + +  A   ID  I + +
Sbjct: 207 TMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAWKVIDSYIYDKI 266

Query: 258 AVKRERLKRARELNEDLQHGDADLITGYI-SFNFH 292
           A  + + K   ++N        D ++ Y+ +FN H
Sbjct: 267 AELKAKRKLVGKIN------SYDAVSFYMDNFNIH 293

BLAST of Lag0012949 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-45
Identity = 99/289 (34.26%), Postives = 160/289 (55.36%), Query Frame = 0

Query: 5   DPFLTIFLASLPF-IFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHV 64
           D +  I +A   F IF L+  + + +   LP+NWPIFGM+P I  + ++ +D  T  +  
Sbjct: 13  DHYPEILIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRK 72

Query: 65  TSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSD 124
              TF FKG W  +  ++ T DP+NI+H+ + NF+ YPKG +   +FD+FG+ IFN+D D
Sbjct: 73  MGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGD 132

Query: 125 SWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFD 184
            W   R++AQ+++    +    A   R K++  L+P+LD        VDL+D+F R  FD
Sbjct: 133 LWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFD 192

Query: 185 STCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMR 244
           ++C  V   D  SL++EFP +PFSKA D   +    RH  PR+ W+L +  ++G  + + 
Sbjct: 193 TSCFSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLA 252

Query: 245 KATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI-SFNFH 292
            A   ID  I + +A  + + K   ++N        D ++ Y+ +FN H
Sbjct: 253 VAWKVIDSYIYDKIAELKAKRKLVGKIN------SYDAVSFYMDNFNIH 293

BLAST of Lag0012949 vs. ExPASy Swiss-Prot
Match: A0A140IL92 (Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T3 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.1e-45
Identity = 99/289 (34.26%), Postives = 159/289 (55.02%), Query Frame = 0

Query: 5   DPFLTIFLASLPF-IFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHV 64
           D +  I +A   F IF L+  + + +   LP+NWPIFGM+P I  +  + +D  T  +  
Sbjct: 13  DHYPEILIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-SQFNDFTTARLRK 72

Query: 65  TSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSD 124
              TF FKG W  +  ++ T DP+NI+H+ + NF+ YPKG +   +FD+FG+ IFN+D D
Sbjct: 73  MGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGD 132

Query: 125 SWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFD 184
            W   R++AQ+++    +    A   R K++  L+P+LD        VDL+D+F R  FD
Sbjct: 133 LWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFD 192

Query: 185 STCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMR 244
           ++C  V   D  SL++EFP +PFSKA D   +    RH  PR+ W+L +  ++G  + + 
Sbjct: 193 TSCFSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLA 252

Query: 245 KATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI-SFNFH 292
            A   ID  I + +A  + + K   ++N        D ++ Y+ +FN H
Sbjct: 253 VAWKVIDSYIYDKIAELKAKRKLVGKIN------SYDAVSFYMDNFNIH 293

BLAST of Lag0012949 vs. ExPASy Swiss-Prot
Match: A0A140IL91 (Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.5e-44
Identity = 99/289 (34.26%), Postives = 160/289 (55.36%), Query Frame = 0

Query: 5   DPFLTIFLASLPF-IFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHV 64
           D +  I +A   F IF L+  + + +   LP+NWPIFGM+P I  + ++ +D  T  +  
Sbjct: 13  DHYPEILIAIACFLIFSLLLSARSSSEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRK 72

Query: 65  TSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSD 124
              TF FKG W  +  ++ T DP+NI+H+ + NF+ YPKG +   +FD+FG+ IFN+D D
Sbjct: 73  MGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGD 132

Query: 125 SWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFD 184
            W   R++AQ+++    +    A   R K++  L+P+LD        VDL+D+ LR  FD
Sbjct: 133 LWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRKPVDLQDVLLRFTFD 192

Query: 185 STCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMR 244
           ++C  V   D  SL++EFP +PFSKA D   +    RH  PR+ W+L +  ++G  + + 
Sbjct: 193 TSCFSVLAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLA 252

Query: 245 KATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI-SFNFH 292
            A   ID  I + +A  + + K   ++N        D ++ Y+ +FN H
Sbjct: 253 VAWKVIDSYIYDKIAELKAKRKLVGKIN------SYDAVSFYMDNFNIH 293

BLAST of Lag0012949 vs. ExPASy TrEMBL
Match: A0A6J1HFA2 (alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111463446 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.7e-129
Identity = 227/285 (79.65%), Postives = 252/285 (88.42%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHKRNPHGIPWNWPIVGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVHEKFL+FMAGAAREKVEKGL+PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHEKFLKFMAGAAREKVEKGLIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP+MYWEL+KKLD+GEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKMYWELIKKLDVGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKA +TID VIANL+AVK ERL RARE+ E+L  G ADLIT Y+
Sbjct: 243 MRKAAETIDHVIANLLAVKTERLNRAREMKEELHDGGADLITWYM 286

BLAST of Lag0012949 vs. ExPASy TrEMBL
Match: A0A6J1KPC4 (alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497483 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.2e-128
Identity = 227/285 (79.65%), Postives = 251/285 (88.07%), Query Frame = 0

Query: 2   ASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 61
           ASLD  L IFLAS P IFIL+ L    NP+G+PWNWPI GMIPT+  +IHRIHDR TEI+
Sbjct: 3   ASLD-LLPIFLASFPLIFILISLFHKRNPHGIPWNWPILGMIPTVIANIHRIHDRITEIL 62

Query: 62  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 121
            VT CTF FKGIWFT TGFLMTVDPANIHHIMST+F+KYPKG +FKYIF+V GDGIFNSD
Sbjct: 63  QVTGCTFHFKGIWFTKTGFLMTVDPANIHHIMSTHFEKYPKGSEFKYIFEVLGDGIFNSD 122

Query: 122 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 181
           +DSWK QRR+AQSLIVHEKFL+FMAGAAREKVEKGL+PVL+F+C+NG VVDLKDLFLRLA
Sbjct: 123 ADSWKNQRRVAQSLIVHEKFLKFMAGAAREKVEKGLIPVLEFYCKNGEVVDLKDLFLRLA 182

Query: 182 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 241
           FDSTCMMVTGFDLNSL LEFPEIPFSKAMDDVQEVLFLRH YP+MYWEL+KKLDIGEAKR
Sbjct: 183 FDSTCMMVTGFDLNSLCLEFPEIPFSKAMDDVQEVLFLRHLYPKMYWELMKKLDIGEAKR 242

Query: 242 MRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           MRKA +TID VIANL+AVK ERL R RE+ E+L  G ADLIT Y+
Sbjct: 243 MRKAAETIDHVIANLLAVKTERLNRDREIKEELHDGGADLITWYM 286

BLAST of Lag0012949 vs. ExPASy TrEMBL
Match: A0A5D3CN31 (Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005620 PE=3 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 8.5e-128
Identity = 225/286 (78.67%), Postives = 250/286 (87.41%), Query Frame = 0

Query: 1   MASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEI 60
           MASLD  L I L SLPFIFI   L L  NP+G+PWNWPIFGMIPT+  HIHR HDR TE+
Sbjct: 1   MASLDLLLPISLISLPFIFIFFSLFLKWNPHGIPWNWPIFGMIPTVILHIHRAHDRITEV 60

Query: 61  MHVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNS 120
           +  TSCTF+FKGIWFTNTGFLMTVDP+NIHHIMSTNF  YPKGPDFKYIFDV GDGIFNS
Sbjct: 61  LQQTSCTFYFKGIWFTNTGFLMTVDPSNIHHIMSTNFHNYPKGPDFKYIFDVLGDGIFNS 120

Query: 121 DSDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRL 180
           DSDSWK QR+IAQSLIVHEKFL FMA AA+EKVEKGL+P+L++FCE+ +VVDLKDLFLRL
Sbjct: 121 DSDSWKNQRKIAQSLIVHEKFLEFMARAAKEKVEKGLVPILEYFCESKNVVDLKDLFLRL 180

Query: 181 AFDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAK 240
           AFDSTCMMVTGFDLNSLS+EFPEIPFSKAMDDVQEV+FLRH YP+ YWELLKKL IG+ K
Sbjct: 181 AFDSTCMMVTGFDLNSLSIEFPEIPFSKAMDDVQEVIFLRHLYPKFYWELLKKLGIGDGK 240

Query: 241 RMRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           RM+KA D ID+VIANLMAVKRERL+R RE+ E+  HG ADLIT Y+
Sbjct: 241 RMKKACDIIDEVIANLMAVKRERLQRDREVKEEQDHG-ADLITWYM 285

BLAST of Lag0012949 vs. ExPASy TrEMBL
Match: A0A0A0KFE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487020 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.9e-126
Identity = 221/286 (77.27%), Postives = 247/286 (86.36%), Query Frame = 0

Query: 1   MASLDPFLTIFLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEI 60
           MAS D  L IFL SLP IFI   L L  NP+G+PWNWP+ GMIPT+ H+I+R+HDR TEI
Sbjct: 1   MASHDLLLPIFLISLPLIFIFFSLFLKWNPHGIPWNWPVLGMIPTVIHNIYRVHDRITEI 60

Query: 61  MHVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNS 120
           +  TSCTF+F GIWFTNTGFLMTVDP+NIHHIMSTNFQ YPKGP+FKYIFDV GDGIFNS
Sbjct: 61  LQQTSCTFYFNGIWFTNTGFLMTVDPSNIHHIMSTNFQNYPKGPEFKYIFDVLGDGIFNS 120

Query: 121 DSDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRL 180
           DSDSWK+QR+IAQSLIVHEKFL FMA AA+EKVEKGL+P+L++FCE+  VVDLKDLFLRL
Sbjct: 121 DSDSWKSQRKIAQSLIVHEKFLEFMARAAKEKVEKGLVPILEYFCESKKVVDLKDLFLRL 180

Query: 181 AFDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAK 240
           AFDSTCMMVTGF LNSL +EFPEIPFSKAMDDVQEVLFLRH YP+ YWELLKKL IGE K
Sbjct: 181 AFDSTCMMVTGFHLNSLCIEFPEIPFSKAMDDVQEVLFLRHLYPKFYWELLKKLGIGEGK 240

Query: 241 RMRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
           RM+KA D ID+VIANLMAVKRERL+R RE+ ED Q   ADLIT Y+
Sbjct: 241 RMKKACDIIDEVIANLMAVKRERLQRDREVKEDQQDHGADLITWYM 286

BLAST of Lag0012949 vs. ExPASy TrEMBL
Match: A0A6J1BWK7 (alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 5.2e-101
Identity = 179/280 (63.93%), Postives = 226/280 (80.71%), Query Frame = 0

Query: 8   LTIFLASLPFIFILV-FLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSC 67
           L+I L SLP IFIL+ F+SLN NP+G+PWNWPI GMIPTIF ++HRIHDR TEI+H +S 
Sbjct: 7   LSILLPSLPLIFILIFFISLNWNPHGIPWNWPILGMIPTIFSNLHRIHDRVTEILHQSSS 66

Query: 68  TFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWK 127
           TFFFK IWF N  FL+T DP+NIHHI+S NFQ+YPKGPDF YIFDV GDGIFN+DSDSWK
Sbjct: 67  TFFFKAIWFANIDFLLTADPSNIHHILSANFQRYPKGPDFNYIFDVLGDGIFNADSDSWK 126

Query: 128 TQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTC 187
            QR+IA SL+  ++FLRF+     +KV++G++ VL+  CENGSV+DL+DLF R +FDSTC
Sbjct: 127 YQRKIAHSLVHRDEFLRFLEKNTMKKVKEGVVSVLESVCENGSVLDLQDLFQRFSFDSTC 186

Query: 188 MMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKAT 247
           M+VTGFDLNSLSL+FP++PFSKAMDDV+E++FLRHF+P+  WELL KL IG+ +R ++A 
Sbjct: 187 MLVTGFDLNSLSLDFPQVPFSKAMDDVEEIIFLRHFFPKALWELLNKLQIGQPRRQKQAR 246

Query: 248 DTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYI 287
             IDQ IA L+A+KRE L++     E+   G ADLIT Y+
Sbjct: 247 QIIDQTIAELIALKRESLQK-----EEAAQG-ADLITSYM 280

BLAST of Lag0012949 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 253.4 bits (646), Expect = 2.2e-67
Identity = 122/286 (42.66%), Postives = 185/286 (64.69%), Query Frame = 0

Query: 7   FLTIFLASLPFIFILVFLSLNLNPYG---LPWNWPIFGMIPTIFHHIHRIHDRFTEIMHV 66
           F+++F  S+ F   L+F    +N       P NWP FGMIP +   IHR++D  TEI+ V
Sbjct: 3   FISLFEISIAFFCFLLFRHFLINKKTHRLCPTNWPFFGMIPGLLVEIHRVYDFITEILEV 62

Query: 67  TSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSD 126
           T+ T+   G  F N   L+TVDPANIHHIMS+NF  YPKGP+FK +FD+ GDGIFN+DS+
Sbjct: 63  TNLTYPCTGPCFANLDMLVTVDPANIHHIMSSNFANYPKGPEFKKLFDILGDGIFNADSE 122

Query: 127 SWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFD 186
            WK  R+ AQS++++ +F +F    + +K+EKGL+P+LD   +    VDL+D+F R  FD
Sbjct: 123 LWKDLRKSAQSMMMNPEFQKFSLATSLKKLEKGLVPLLDHVAKEKLAVDLQDMFQRFTFD 182

Query: 187 STCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMR 246
           +T ++ TG+D   LS+E PE+ F++A+DD +E +F RH  P ++W L   L +G+ K+M 
Sbjct: 183 TTFVLATGYDPGCLSVEMPEVEFARALDDAEEAIFFRHVKPEIFWRLQGLLGLGDEKKMT 242

Query: 247 KATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYISFN 290
           KA  T+D+V +  +A+KR+ + R      ++     DL+T Y++ +
Sbjct: 243 KARSTLDRVCSKYIAIKRDEVSRG---TNNVDSHSKDLLTSYMNLD 285

BLAST of Lag0012949 vs. TAIR 10
Match: AT1G47620.1 (cytochrome P450, family 96, subfamily A, polypeptide 8 )

HSP 1 Score: 231.5 bits (589), Expect = 8.8e-61
Identity = 116/279 (41.58%), Postives = 167/279 (59.86%), Query Frame = 0

Query: 11  FLASLPFIFILVFLSLNLNPYGLPWNWPIFGMIPTIFHHIHRIHDRFTEIMHVTSCTFFF 70
           FL   PF ++L+  +L       PWNWP+ GM+P +   + RI+D   E++  ++ TF F
Sbjct: 22  FLVKKPFSYLLIKKTLQ----SYPWNWPVLGMLPGVLLRLQRIYDCSVEVLENSNMTFQF 81

Query: 71  KGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWKTQRR 130
           KG WF     L TVDPANIHHIMS+NF  Y KGP F  IF+ FGDGI N+D++ W+  R 
Sbjct: 82  KGPWFVGMDVLATVDPANIHHIMSSNFSNYIKGPIFHEIFEAFGDGIINTDAELWRDWRN 141

Query: 131 IAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTCMMVT 190
            +Q +  H+++  F A   + KV  GL+P+ + F     VVDL+D+F R  +D T + +T
Sbjct: 142 ASQLIFNHQRYQNFSASTTKTKVNDGLVPLFNHFANEEIVVDLEDVFQRFMYDITFIFIT 201

Query: 191 GFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKATDTID 250
           G D  SLS+E PE+ FSKA+DDV + +  RH  PR  W+L K + IG  K+M KA  T D
Sbjct: 202 GTDPRSLSIEMPEVEFSKALDDVGDAIVHRHITPRFVWKLQKWIGIGTEKKMLKAHATFD 261

Query: 251 QVIANLMAVKRERLKRARELNEDLQHGDADLITGYISFN 290
           +V   ++A KRE L  ++ +  +      DL+T +I  +
Sbjct: 262 RVCEKIIAAKREEL-GSQGITYNSNGEREDLLTSFIKLD 295

BLAST of Lag0012949 vs. TAIR 10
Match: AT4G39510.1 (cytochrome P450, family 96, subfamily A, polypeptide 12 )

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-60
Identity = 116/283 (40.99%), Postives = 174/283 (61.48%), Query Frame = 0

Query: 8   LTIFLASLPFIFILVFLSLNLNPYGLPW-NWPIFGMIPTIFHHIHRIHDRFTEIMHVTSC 67
           + I +  L F + + F      P+G  + NWP+ GM+P     +HRI++   E + ++  
Sbjct: 9   VAIAIICLFFFYYMYF----KKPHGQVFRNWPVIGMLPGFLMVLHRIYNFGVEALEMSHL 68

Query: 68  TFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSDSDSWK 127
           TF FKG WF     L TVDPANIH+I+S+NF  Y KG DFK +FDVFG+ IF+SDS+ WK
Sbjct: 69  TFLFKGPWFAEMDMLFTVDPANIHYILSSNFSNYTKGADFKEVFDVFGEMIFSSDSELWK 128

Query: 128 TQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLAFDSTC 187
            QR+ AQ ++ H+ F +    A R K+  GL+P+ +  CE   VVDL+ +F R  FD+T 
Sbjct: 129 NQRKAAQFMLNHQGFQKLSLSATRSKLYDGLVPLFNQCCEEEKVVDLQQVFQRFTFDTTF 188

Query: 188 MMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKRMRKAT 247
            +VTGFD  SLS+E PE+ ++KA+DD+ E +F RH  P+ +W+L  +  +G+ KRM +A 
Sbjct: 189 FIVTGFDPKSLSIEMPEVEYAKALDDLGEGIFYRHIKPKFFWKLQNRFGLGQEKRMTEAD 248

Query: 248 DTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYISFN 290
            T D+V A  +  KRE + R++ ++        DL+T +I  +
Sbjct: 249 ATFDRVSAKYILAKREEI-RSQGIDHHANGESEDLLTSHIKLD 286

BLAST of Lag0012949 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 226.1 bits (575), Expect = 3.7e-59
Identity = 110/263 (41.83%), Postives = 167/263 (63.50%), Query Frame = 0

Query: 3   SLDPFLTIFLASLPFIFILVFLSLNLNPY-GLPWNWPIFGMIPTIFHHIHRIHDRFTEIM 62
           +L   L + ++ L F F+  +  ++  P+     NWP  GM+P +   I R++D  TE++
Sbjct: 2   ALITLLEVSISLLFFSFLYGYFLISKKPHRSFLTNWPFLGMLPGLLVEIPRVYDFVTELL 61

Query: 63  HVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFNSD 122
             ++ T+ FKG  F     L+TVDPANIHHIMS+NF  YPKG +FK IFDV GDGIFN+D
Sbjct: 62  EASNLTYPFKGPCFGGLDMLITVDPANIHHIMSSNFANYPKGTEFKKIFDVLGDGIFNAD 121

Query: 123 SDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLRLA 182
           S+ WK  R+ AQS++ H+ F RF       K+EKGL+P+LD+  E   VVDL+D+F R  
Sbjct: 122 SELWKDLRKSAQSMMTHQDFQRFTLRTIMSKLEKGLVPLLDYVAEKKQVVDLQDVFQRFT 181

Query: 183 FDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEAKR 242
           FD++ ++ TG D   LS E P+I F++A+D+ +E +F RH  P + W++ + +  G+  +
Sbjct: 182 FDTSFVLATGVDPGCLSTEMPQIEFARALDEAEEAIFFRHVKPEIVWKMQRFIGFGDELK 241

Query: 243 MRKATDTIDQVIANLMAVKRERL 265
           M+KA  T D+V +  +A KR+ +
Sbjct: 242 MKKAHSTFDRVCSKCIASKRDEI 264

BLAST of Lag0012949 vs. TAIR 10
Match: AT4G32170.1 (cytochrome P450, family 96, subfamily A, polypeptide 2 )

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-58
Identity = 118/290 (40.69%), Postives = 177/290 (61.03%), Query Frame = 0

Query: 1   MASLDPFLTIFLASLPFIFILVFLSLNLNPYG-LPWNWPIFGMIPTIFHHIHRIHDRFTE 60
           MAS+   L + +A L F+ +  FL     P+G  P NWP+  M+P +   +HRI+D   +
Sbjct: 1   MASIS-LLQVSVAILCFLILHYFLF--KKPHGRYPRNWPVLRMLPAMLKALHRIYDYSVK 60

Query: 61  IMHVTSCTFFFKGIWFTNTGFLMTVDPANIHHIMSTNFQKYPKGPDFKYIFDVFGDGIFN 120
           I+  +  TF FKG  FT    L+TVDPANIHHIMS+NF  Y KGP+F+ +FDV GD    
Sbjct: 61  ILETSDLTFPFKGPRFTGMDMLLTVDPANIHHIMSSNFSNYIKGPEFQDVFDVLGDSFIT 120

Query: 121 SDSDSWKTQRRIAQSLIVHEKFLRFMAGAAREKVEKGLMPVLDFFCENGSVVDLKDLFLR 180
           +DS+ WK  R+  Q+++  ++F RF       K++ GL+P+L+ F E G+ +DL+ +F R
Sbjct: 121 TDSELWKNMRKSYQAMLHSQEFQRFSMSTMTSKLKYGLVPLLNHFAEEGTTLDLQSVFGR 180

Query: 181 LAFDSTCMMVTGFDLNSLSLEFPEIPFSKAMDDVQEVLFLRHFYPRMYWELLKKLDIGEA 240
             FD+  ++VTG D  SLS+E PE  F+KA+DDV E +  RHF PR  W+L   +  G+ 
Sbjct: 181 FTFDTIFILVTGSDPRSLSIEMPEDEFAKALDDVGEGILYRHFKPRFLWKLQNWIGFGQE 240

Query: 241 KRMRKATDTIDQVIANLMAVKRERLKRARELNEDLQHGDADLITGYISFN 290
           K++ +A  T D+V A  ++ KRE +KR++  +     G  DL+T +I  +
Sbjct: 241 KKLTEANATFDRVCAKYISAKREEIKRSQGTSNG---GSQDLLTSFIKLD 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882847.17.1e-12981.56alkane hydroxylase MAH1-like [Benincasa hispida][more]
XP_022963151.11.6e-12879.65alkane hydroxylase MAH1-like [Cucurbita moschata][more]
KAG6595090.12.1e-12879.30Alkane hydroxylase MAH1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023004057.14.6e-12879.65alkane hydroxylase MAH1-like [Cucurbita maxima][more]
XP_023518531.11.0e-12778.95alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FVS93.6e-5138.61Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1[more]
A0A2H5AJ002.3e-4534.55Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=... [more]
A0A140IL903.0e-4534.26Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G... [more]
A0A140IL925.1e-4534.26Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A140IL911.5e-4434.26Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
Match NameE-valueIdentityDescription
A0A6J1HFA27.7e-12979.65alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111463446 PE=3 ... [more]
A0A6J1KPC42.2e-12879.65alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111497483 PE=3 SV... [more]
A0A5D3CN318.5e-12878.67Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0KFE27.9e-12677.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487020 PE=3 SV=1[more]
A0A6J1BWK75.2e-10163.93alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G39490.12.2e-6742.66cytochrome P450, family 96, subfamily A, polypeptide 10 [more]
AT1G47620.18.8e-6141.58cytochrome P450, family 96, subfamily A, polypeptide 8 [more]
AT4G39510.11.5e-6040.99cytochrome P450, family 96, subfamily A, polypeptide 12 [more]
AT2G23180.13.7e-5941.83cytochrome P450, family 96, subfamily A, polypeptide 1 [more]
AT4G32170.11.4e-5840.69cytochrome P450, family 96, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 242..262
NoneNo IPR availablePANTHERPTHR24296:SF193ALKANE HYDROXYLASE MAH1-LIKEcoord: 13..289
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 13..289
IPR001128Cytochrome P450PFAMPF00067p450coord: 35..275
e-value: 2.5E-12
score: 46.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..289
e-value: 1.6E-30
score: 108.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 26..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012949.1Lag0012949.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen