Lag0012842 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012842 vs. NCBI nr
Match: TYK00226.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 349.7 bits (896), Expect = 4.4e-92
Identity = 230/681 (33.77%), Postives = 323/681 (47.43%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PI  S   +L   F E E+ + L +   NK+PGPDG+T+EF KK W  +K  
Sbjct: 30  LLENLNWTPIDSSLHVALCSEFKESEIKKALFSFNGNKAPGPDGYTMEFLKKFWNHLKGD 89

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           V++VF DF++K IIN NVN TYI LI KK+     +D+RSISL T LY++IAK +A RLK
Sbjct: 90  VIKVFKDFHEKSIINKNVNNTYIALIAKKSNCANPKDYRSISLATSLYKIIAKAIANRLK 149

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
           STL  ++S NQLAFVKGRQIT AIL+ANE VDFW  S T+G+V+KLDIEKAFDK+ W+FI
Sbjct: 150 STLPTSISPNQLAFVKGRQITYAILMANEAVDFWITSKTKGYVLKLDIEKAFDKVRWDFI 209

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  K YP+ W                                               
Sbjct: 210 DYMLRVKNYPLKWRSWIKACISSIQYSILINGRPRGKILPNRGLRQGDPISPFIFVIAMD 269

Query: 241 ---------------------------------DILLFVQDNDAMLGNLFNIIKVFELSS 300
                                            DIL+F++D++  + +L N I +FE +S
Sbjct: 270 YLSRLLTKLQKNGVIKGVVFDKNCELNYLLFVDDILVFIEDDNRAIKSLQNAIFLFEAAS 329

Query: 301 GLNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIRLSDLFFG---MGK 360
           GL IN SKSSI+ +N+   R A++A  W  P    P  YL  PLG + +   F    M K
Sbjct: 330 GLTINRSKSSISPVNIPTTRSAEVANLWNFPTKLLPKEYLLVPLGGKPNTKGFWTNIMDK 389

Query: 361 IKVVASLW----LIGAWWLRLLRLGVWAYSRLELQIAHFKS-NGFG---DSFMRRLL--- 420
           I+   S W    +     L L++  + +    +L I  FK+ +G G   +   R  L   
Sbjct: 390 IQKKLSGWKYSQISKGGKLTLIQASLSSLPTYQLSI--FKALSGVGKTIEKCWRNFLWKN 449

Query: 421 -------------------CGSESSQQNIQPIDKEL-------FQLRRDSL--------- 480
                              C       N++ I+  L       FQ  +DSL         
Sbjct: 450 KNETNCTNLARWSTITTPKCKGGLGITNVKVINTALLCKWLWRFQEEKDSLWVEIISAKY 509

Query: 481 --------PP--------RAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLY 530
                   PP        +APW  I+K+   F  ++ W +KDG  +SFWH +W   G L 
Sbjct: 510 KGHHIGDFPPTIGKYSSMKAPWRQIIKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLS 569

BLAST of Lag0012842 vs. NCBI nr
Match: KAA0046762.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 344.4 bits (882), Expect = 1.9e-90
Identity = 215/645 (33.33%), Postives = 297/645 (46.05%), Query Frame = 0

Query: 2   VSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSV 61
           + NL W PI  SE  +L  PF E E+   + ++   K+PGPDGF + FFK  W  +K  +
Sbjct: 258 IDNLEWNPIEHSEGLNLCAPFLEDEIKGVINSLDGKKTPGPDGFPISFFKSYWYLLKEDI 317

Query: 62  MEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLKS 121
           M++F DFY K +IN N+N TYI LIPKK      +DFR ISLTT +Y++IAKTL+ RLK+
Sbjct: 318 MDIFKDFYDKGVINKNMNNTYIALIPKKKDFSNPKDFRPISLTTSIYKIIAKTLSNRLKT 377

Query: 122 TLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFID 181
           +L  T+SENQLAFVK RQITDAIL+ANE VDFWK    +GF++KLDIEKAFD + W+FID
Sbjct: 378 SLPNTISENQLAFVKNRQITDAILMANEAVDFWKVKKIKGFILKLDIEKAFDNLNWDFID 437

Query: 182 KILAFKGYPITWH----------------------------------------------- 241
            +L  K +PI W                                                
Sbjct: 438 FVLEKKNFPILWRKWIRGCISNVTYSIIVNGRPQGRIKANRGLRQGDPLSPFLFVIAMDY 497

Query: 242 --------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSG 301
                                           DILLF++DND  L NL   + +FE +SG
Sbjct: 498 LSRLLSHLEITGAIKGVSFSSNCNISHILFADDILLFIEDNDFFLNNLRMALSLFERASG 557

Query: 302 LNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIR-LSDLFFGMGKIKV 361
           L IN  KS++  +NV ++R  + A+ WG      P+ YLG PLG    S LF+   + K+
Sbjct: 558 LKINLLKSALVPMNVSEIRAKECASFWGISSQSLPLSYLGVPLGGNPKSRLFWSNVEEKI 617

Query: 362 VASL--W----LIGAWWLRLLRLGVWAYSRLELQIAHFKSNGFG--DSFMRRLLCGSESS 421
              L  W    +     L L++  + +    +L +    S      + F R  L    +S
Sbjct: 618 QKKLNNWKYAQISKGGRLTLIKSTLSSLPTYQLSVFQAPSLTCKNIEKFWRNFLWKGNNS 677

Query: 422 QQNIQPID-----------------------------------------KELFQLRRDSL 481
            +    I+                                         + L Q +   +
Sbjct: 678 SEGFHLINWTKVTKSKAEGGLGISRVHVTNKALLTKWLWRYLSEPKALWRRLIQCKYKGI 737

Query: 482 PP------------RAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAIP 506
            P            +APW SI+     F +N  W+L +G +ISFW+ +W+  G L  A P
Sbjct: 738 YPGDIPSNISSSTSKAPWRSIIDNVDWFRSNQSWDLNNGDQISFWYSNWSIEGRLLTAYP 797

BLAST of Lag0012842 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 344.4 bits (882), Expect = 1.9e-90
Identity = 225/649 (34.67%), Postives = 309/649 (47.61%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PIS ++A +L   F+E E+ + L A  +NKSPGPDGFT+EF+K +W  +K  
Sbjct: 257 LIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEE 316

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           ++ +F DF+   IIN  VN T I LI KK    +  D+R ISLTT +Y+LIAK +AERLK
Sbjct: 317 ILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLK 376

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
            TL  TV+ENQ+AFVKGRQI DAILVANE +D+W+    +GFVIKLDIEKAFDK+ W FI
Sbjct: 377 DTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFI 436

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  KGYP  W                                               
Sbjct: 437 DFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMD 496

Query: 241 -------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSGL 300
                                          DILLFV+D++  + NL NII +F+L+SGL
Sbjct: 497 YMSRLLNSVGEKIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGL 556

Query: 301 NINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLG----------------- 360
           +IN +KS+I+ INV+  R  QIA+ WG      PI YLG PLG                 
Sbjct: 557 SINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKIN 616

Query: 361 IRLSDLFFGM----GKIKVV-ASLWLIGAWWLRLLRLGVWAYSRLE-------------- 420
            +L+   + M    GKI ++ +SL  +  + L + ++ V     +E              
Sbjct: 617 KKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPET 676

Query: 421 -------------------LQIAHFKSNGFG--DSFMRRLLCGSESSQQNIQPIDKELFQ 480
                              L I+  K   F     ++ R +   E S    + I+ +   
Sbjct: 677 HKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYI--HEDSPLWKKIINAKYRS 736

Query: 481 LRRDSLP-------PRAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAI 506
           L +  +P        R+PW SI K    F  +  W +K+G   SFWH  W  + PL    
Sbjct: 737 LSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHY 796

BLAST of Lag0012842 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 343.6 bits (880), Expect = 3.2e-90
Identity = 225/649 (34.67%), Postives = 308/649 (47.46%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PIS ++A +L   F+E E+ + L A  +NKSPGPDGFT+EF+K +W  +K  
Sbjct: 257 LIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEE 316

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           ++ +F DF+   IIN  VN T I LI KK    +  D+R ISLTT +Y+LIAK +AERLK
Sbjct: 317 ILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLK 376

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
            TL  TV+ENQ+AFVKGRQI DAILVANE +D+W+    +GFVIKLDIEKAFDK+ W FI
Sbjct: 377 DTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFI 436

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  KGYP  W                                               
Sbjct: 437 DFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMD 496

Query: 241 -------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSGL 300
                                          DILLFV+D++  + NL NII +F+L+SGL
Sbjct: 497 YMSRLLNSVGEKIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGL 556

Query: 301 NINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLG----------------- 360
           +IN +KS+I+ INV+  R  QIA+ WG      PI YLG PLG                 
Sbjct: 557 SINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKIN 616

Query: 361 IRLSDLFFGM----GKIKVV-ASLWLIGAWWLRLLRLGVWAYSRLE-------------- 420
            +L+   + M    GKI ++ +SL  +  + L + +  V     +E              
Sbjct: 617 KKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPET 676

Query: 421 -------------------LQIAHFKSNGFG--DSFMRRLLCGSESSQQNIQPIDKELFQ 480
                              L I+  K   F     ++ R +   E S    + I+ +   
Sbjct: 677 HKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYI--HEDSPLWKKIINAKYRS 736

Query: 481 LRRDSLP-------PRAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAI 506
           L +  +P        R+PW SI K    F  +  W +K+G   SFWH  W  + PL    
Sbjct: 737 LSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHY 796

BLAST of Lag0012842 vs. NCBI nr
Match: KAA0037436.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 340.1 bits (871), Expect = 3.5e-89
Identity = 230/706 (32.58%), Postives = 323/706 (45.75%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PI  S   +L   F E E+ + L +   NK+PGPDG+T+EF KK W  +K  
Sbjct: 30  LLENLNWTPIDSSLHVALCSEFKESEIKKALFSFNGNKAPGPDGYTMEFLKKFWNHLKGD 89

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           V++VF DF++K IIN NVN TYI LI KK+     +D+RSISL T LY++IAK +A RLK
Sbjct: 90  VIKVFKDFHEKSIINKNVNNTYIALIAKKSNCANPKDYRSISLATSLYKIIAKAIANRLK 149

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
           STL  ++S NQLAFVKGRQIT AIL+ANE VDFW  S T+G+V+KLDIEKAFDK+ W+FI
Sbjct: 150 STLPTSISPNQLAFVKGRQITYAILMANEAVDFWITSKTKGYVLKLDIEKAFDKVRWDFI 209

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  K YP+ W                                               
Sbjct: 210 DYMLRVKNYPLKWRSWIKACISSIQYSILINGRPRGKILPNRGLRQGDPISPFIFVIAMD 269

Query: 241 ---------------------------------DILLFVQDNDAMLGNLFNIIKVFELSS 300
                                            DIL+F++D++  + +L N I +FE +S
Sbjct: 270 YLSRLLTKLQKNGVIKGVVFDKNCELNYLLFVDDILVFIEDDNRAIKSLQNAIFLFEAAS 329

Query: 301 GLNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIRLSDLFFG---MGK 360
           GL IN SKSSI+ +N+   R A++A  W  P    P  YL  PLG + +   F    M K
Sbjct: 330 GLTINRSKSSISPVNIPTTRSAEVANLWNFPTKLLPKEYLLVPLGGKPNTKGFWTNIMDK 389

Query: 361 IKVVASLW----LIGAWWLRLLRLGVWAYSRLELQIAHFKS-NGFG-------------- 420
           I+   S W    +     L L++  + +    +L I  FK+ +G G              
Sbjct: 390 IQKKLSGWKYSQISKGGKLTLIQASLSSLPTYQLSI--FKALSGVGKTIEKCWRNFLWKN 449

Query: 421 --------------DSFMRRLL----------------------CGSESSQQNIQPIDKE 480
                         +   R  L                      C       N++ I+  
Sbjct: 450 KNETNCTNLAGKTIEKCWRNFLWKNKNETNCTNLARWSTITTPKCKGGLGITNVKVINTA 509

Query: 481 L-------FQLRRDSL-----------------PP--------RAPWTSILKQASLFLAN 530
           L       FQ  +DSL                 PP        +APW  I+K+   F  +
Sbjct: 510 LLCKWLWRFQEEKDSLWVEIISAKYKGHHIGDFPPTIGKYSSMKAPWRQIIKEIGWFQNH 569

BLAST of Lag0012842 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-18
Identity = 53/166 (31.93%), Postives = 90/166 (54.22%), Query Frame = 0

Query: 10  ISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFY 69
           +S      L  P +  E+ Q L+ M HNKSPG DG T+EFF+  W  + P    V  + +
Sbjct: 437 VSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAF 496

Query: 70  QKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLKSTLQGTVSE 129
           +K  +  +     ++L+PKK     I+++R +SL +  Y+++AK ++ RLKS L   +  
Sbjct: 497 KKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHP 556

Query: 130 NQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKI 176
           +Q   V GR I D + +  +++ F + +      + LD EKAFD++
Sbjct: 557 DQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRV 602

BLAST of Lag0012842 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 1.2e-15
Identity = 55/181 (30.39%), Postives = 95/181 (52.49%), Query Frame = 0

Query: 10  ISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFY 69
           ++  +   L  P S  E+   + ++   KSPGPDGF+ EF++    D+ P + ++F+   
Sbjct: 448 LNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIE 507

Query: 70  QKDIINCNVNETYITLIPK-KNTALQIQDFRSISLTTVLYRLIAKTLAERLKSTLQGTVS 129
            +  +  +  E  ITLIPK +    +I++FR ISL  +  +++ K LA R++  ++  + 
Sbjct: 508 VEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIH 567

Query: 130 ENQLAFVKGRQITDAILVANEVVDFW-KCSHTRGFVIKLDIEKAFDKICWNFIDKILAFK 189
            +Q+ F+ G Q    I  +  V+ +  K       +I LD EKAFDKI   F+ K+L   
Sbjct: 568 PDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERS 627

BLAST of Lag0012842 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.7e-14
Identity = 54/180 (30.00%), Postives = 96/180 (53.33%), Query Frame = 0

Query: 10  ISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFY 69
           ++  E  SL +P +  E+   + ++   KSPGPDGFT EF+++   ++ P ++++F    
Sbjct: 441 LNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIE 500

Query: 70  QKDIINCNVNETYITLIPKK-NTALQIQDFRSISLTTVLYRLIAKTLAERLKSTLQGTVS 129
           ++ I+  +  E  I LIPK      + ++FR ISL  +  +++ K LA R++  ++  + 
Sbjct: 501 KEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIH 560

Query: 130 ENQLAFVKGRQ----ITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFIDKIL 185
            +Q+ F+ G Q    I  +I V   +      +H    +I +D EKAFDKI   F+ K L
Sbjct: 561 HDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHV---IISIDAEKAFDKIQQPFMLKTL 617

BLAST of Lag0012842 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-13
Identity = 51/180 (28.33%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 10  ISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFY 69
           +S  E   L +P S  E+   ++ +   KSPGPDGFT EF++    ++ P ++ +F +  
Sbjct: 440 LSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNIE 499

Query: 70  QKDIINCNVNETYITLIPKK-NTALQIQDFRSISLTTVLYRLIAKTLAERLKSTLQGTVS 129
           ++ I+     E  ITLIPK      + +++R ISL  +  +++ K L  R++  ++  + 
Sbjct: 500 KEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIKKIIH 559

Query: 130 ENQLAFVKGRQ----ITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFIDKIL 185
            +Q+ F+ G Q    I  +I V   +       H    ++ +D EKAFD I   F+ + L
Sbjct: 560 HDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDH---MILSIDAEKAFDNIQHPFMIRTL 616

BLAST of Lag0012842 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.8e-04
Identity = 47/169 (27.81%), Postives = 73/169 (43.20%), Query Frame = 0

Query: 29  QNLKA--MGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFYQKDIINC-----NVNET 88
           Q+L+A  +  + SPGPDG T +  ++    I   +M +        I+ C     ++   
Sbjct: 347 QDLRASRVSLSSSPGPDGITPKSAREVPSGIMLRIMNL--------ILWCGNLPHSIRLA 406

Query: 89  YITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLKSTLQGTVSENQLAFVKGRQIT 148
               IPK  TA + QDFR IS+ +VL R +   LA RL S++       Q  F+      
Sbjct: 407 RTVFIPKTVTAKRPQDFRPISVPSVLVRQLNAILATRLNSSINW--DPRQRGFLPTDGCA 466

Query: 149 DAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFIDKILAFKGYP 191
           D   + + V+          ++  LD+ KAFD +    I   L   G P
Sbjct: 467 DNATIVDLVLRHSHKHFRSCYIANLDVSKAFDSLSHASIYDTLRAYGAP 505

BLAST of Lag0012842 vs. ExPASy TrEMBL
Match: A0A5D3BPP1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00200 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 2.1e-92
Identity = 230/681 (33.77%), Postives = 323/681 (47.43%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PI  S   +L   F E E+ + L +   NK+PGPDG+T+EF KK W  +K  
Sbjct: 30  LLENLNWTPIDSSLHVALCSEFKESEIKKALFSFNGNKAPGPDGYTMEFLKKFWNHLKGD 89

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           V++VF DF++K IIN NVN TYI LI KK+     +D+RSISL T LY++IAK +A RLK
Sbjct: 90  VIKVFKDFHEKSIINKNVNNTYIALIAKKSNCANPKDYRSISLATSLYKIIAKAIANRLK 149

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
           STL  ++S NQLAFVKGRQIT AIL+ANE VDFW  S T+G+V+KLDIEKAFDK+ W+FI
Sbjct: 150 STLPTSISPNQLAFVKGRQITYAILMANEAVDFWITSKTKGYVLKLDIEKAFDKVRWDFI 209

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  K YP+ W                                               
Sbjct: 210 DYMLRVKNYPLKWRSWIKACISSIQYSILINGRPRGKILPNRGLRQGDPISPFIFVIAMD 269

Query: 241 ---------------------------------DILLFVQDNDAMLGNLFNIIKVFELSS 300
                                            DIL+F++D++  + +L N I +FE +S
Sbjct: 270 YLSRLLTKLQKNGVIKGVVFDKNCELNYLLFVDDILVFIEDDNRAIKSLQNAIFLFEAAS 329

Query: 301 GLNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIRLSDLFFG---MGK 360
           GL IN SKSSI+ +N+   R A++A  W  P    P  YL  PLG + +   F    M K
Sbjct: 330 GLTINRSKSSISPVNIPTTRSAEVANLWNFPTKLLPKEYLLVPLGGKPNTKGFWTNIMDK 389

Query: 361 IKVVASLW----LIGAWWLRLLRLGVWAYSRLELQIAHFKS-NGFG---DSFMRRLL--- 420
           I+   S W    +     L L++  + +    +L I  FK+ +G G   +   R  L   
Sbjct: 390 IQKKLSGWKYSQISKGGKLTLIQASLSSLPTYQLSI--FKALSGVGKTIEKCWRNFLWKN 449

Query: 421 -------------------CGSESSQQNIQPIDKEL-------FQLRRDSL--------- 480
                              C       N++ I+  L       FQ  +DSL         
Sbjct: 450 KNETNCTNLARWSTITTPKCKGGLGITNVKVINTALLCKWLWRFQEEKDSLWVEIISAKY 509

Query: 481 --------PP--------RAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLY 530
                   PP        +APW  I+K+   F  ++ W +KDG  +SFWH +W   G L 
Sbjct: 510 KGHHIGDFPPTIGKYSSMKAPWRQIIKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLS 569

BLAST of Lag0012842 vs. ExPASy TrEMBL
Match: A0A5A7TTK1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G00450 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 9.0e-91
Identity = 215/645 (33.33%), Postives = 297/645 (46.05%), Query Frame = 0

Query: 2   VSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSV 61
           + NL W PI  SE  +L  PF E E+   + ++   K+PGPDGF + FFK  W  +K  +
Sbjct: 258 IDNLEWNPIEHSEGLNLCAPFLEDEIKGVINSLDGKKTPGPDGFPISFFKSYWYLLKEDI 317

Query: 62  MEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLKS 121
           M++F DFY K +IN N+N TYI LIPKK      +DFR ISLTT +Y++IAKTL+ RLK+
Sbjct: 318 MDIFKDFYDKGVINKNMNNTYIALIPKKKDFSNPKDFRPISLTTSIYKIIAKTLSNRLKT 377

Query: 122 TLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFID 181
           +L  T+SENQLAFVK RQITDAIL+ANE VDFWK    +GF++KLDIEKAFD + W+FID
Sbjct: 378 SLPNTISENQLAFVKNRQITDAILMANEAVDFWKVKKIKGFILKLDIEKAFDNLNWDFID 437

Query: 182 KILAFKGYPITWH----------------------------------------------- 241
            +L  K +PI W                                                
Sbjct: 438 FVLEKKNFPILWRKWIRGCISNVTYSIIVNGRPQGRIKANRGLRQGDPLSPFLFVIAMDY 497

Query: 242 --------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSG 301
                                           DILLF++DND  L NL   + +FE +SG
Sbjct: 498 LSRLLSHLEITGAIKGVSFSSNCNISHILFADDILLFIEDNDFFLNNLRMALSLFERASG 557

Query: 302 LNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIR-LSDLFFGMGKIKV 361
           L IN  KS++  +NV ++R  + A+ WG      P+ YLG PLG    S LF+   + K+
Sbjct: 558 LKINLLKSALVPMNVSEIRAKECASFWGISSQSLPLSYLGVPLGGNPKSRLFWSNVEEKI 617

Query: 362 VASL--W----LIGAWWLRLLRLGVWAYSRLELQIAHFKSNGFG--DSFMRRLLCGSESS 421
              L  W    +     L L++  + +    +L +    S      + F R  L    +S
Sbjct: 618 QKKLNNWKYAQISKGGRLTLIKSTLSSLPTYQLSVFQAPSLTCKNIEKFWRNFLWKGNNS 677

Query: 422 QQNIQPID-----------------------------------------KELFQLRRDSL 481
            +    I+                                         + L Q +   +
Sbjct: 678 SEGFHLINWTKVTKSKAEGGLGISRVHVTNKALLTKWLWRYLSEPKALWRRLIQCKYKGI 737

Query: 482 PP------------RAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAIP 506
            P            +APW SI+     F +N  W+L +G +ISFW+ +W+  G L  A P
Sbjct: 738 YPGDIPSNISSSTSKAPWRSIIDNVDWFRSNQSWDLNNGDQISFWYSNWSIEGRLLTAYP 797

BLAST of Lag0012842 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 9.0e-91
Identity = 225/649 (34.67%), Postives = 309/649 (47.61%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PIS ++A +L   F+E E+ + L A  +NKSPGPDGFT+EF+K +W  +K  
Sbjct: 257 LIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEE 316

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           ++ +F DF+   IIN  VN T I LI KK    +  D+R ISLTT +Y+LIAK +AERLK
Sbjct: 317 ILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLK 376

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
            TL  TV+ENQ+AFVKGRQI DAILVANE +D+W+    +GFVIKLDIEKAFDK+ W FI
Sbjct: 377 DTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFI 436

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  KGYP  W                                               
Sbjct: 437 DFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMD 496

Query: 241 -------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSGL 300
                                          DILLFV+D++  + NL NII +F+L+SGL
Sbjct: 497 YMSRLLNSVGEKIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGL 556

Query: 301 NINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLG----------------- 360
           +IN +KS+I+ INV+  R  QIA+ WG      PI YLG PLG                 
Sbjct: 557 SINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKIN 616

Query: 361 IRLSDLFFGM----GKIKVV-ASLWLIGAWWLRLLRLGVWAYSRLE-------------- 420
            +L+   + M    GKI ++ +SL  +  + L + ++ V     +E              
Sbjct: 617 KKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKVPVSTCKNIEKTWRNFLWKNPPET 676

Query: 421 -------------------LQIAHFKSNGFG--DSFMRRLLCGSESSQQNIQPIDKELFQ 480
                              L I+  K   F     ++ R +   E S    + I+ +   
Sbjct: 677 HKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYI--HEDSPLWKKIINAKYRS 736

Query: 481 LRRDSLP-------PRAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAI 506
           L +  +P        R+PW SI K    F  +  W +K+G   SFWH  W  + PL    
Sbjct: 737 LSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHY 796

BLAST of Lag0012842 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 1.5e-90
Identity = 225/649 (34.67%), Postives = 308/649 (47.46%), Query Frame = 0

Query: 1   MVSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPS 60
           ++ NLNW PIS ++A +L   F+E E+ + L A  +NKSPGPDGFT+EF+K +W  +K  
Sbjct: 257 LIDNLNWSPISTNQAQNLCSMFTEEEIHEALTAFSNNKSPGPDGFTMEFYKSTWSVLKEE 316

Query: 61  VMEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLK 120
           ++ +F DF+   IIN  VN T I LI KK    +  D+R ISLTT +Y+LIAK +AERLK
Sbjct: 317 ILNIFRDFHSNCIINKAVNITNIALIAKKEKCAEPADYRPISLTTSIYKLIAKVIAERLK 376

Query: 121 STLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFI 180
            TL  TV+ENQ+AFVKGRQI DAILVANE +D+W+    +GFVIKLDIEKAFDK+ W FI
Sbjct: 377 DTLPYTVAENQMAFVKGRQIIDAILVANEAIDYWRVKKIQGFVIKLDIEKAFDKLNWRFI 436

Query: 181 DKILAFKGYPITWH---------------------------------------------- 240
           D +L  KGYP  W                                               
Sbjct: 437 DFMLMKKGYPFKWRNWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMD 496

Query: 241 -------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSGL 300
                                          DILLFV+D++  + NL NII +F+L+SGL
Sbjct: 497 YMSRLLNSVGEKIKGVKLEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLASGL 556

Query: 301 NINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLG----------------- 360
           +IN +KS+I+ INV+  R  QIA+ WG      PI YLG PLG                 
Sbjct: 557 SINLNKSTISPINVDASRTEQIASQWGISTKFLPINYLGVPLGGKQITKTFWKNVEEKIN 616

Query: 361 IRLSDLFFGM----GKIKVV-ASLWLIGAWWLRLLRLGVWAYSRLE-------------- 420
            +L+   + M    GKI ++ +SL  +  + L + +  V     +E              
Sbjct: 617 KKLASWKYSMLSKGGKITLIKSSLASLPTYQLSIFKAPVSTCKNIEKTWRNFLWKNPPET 676

Query: 421 -------------------LQIAHFKSNGFG--DSFMRRLLCGSESSQQNIQPIDKELFQ 480
                              L I+  K   F     ++ R +   E S    + I+ +   
Sbjct: 677 HKLHLVNWAKITSSKEKGGLGISRLKDTNFALLTKWLWRYI--HEDSPLWKKIINAKYRS 736

Query: 481 LRRDSLP-------PRAPWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAI 506
           L +  +P        R+PW SI K    F  +  W +K+G   SFWH  W  + PL    
Sbjct: 737 LSKGDIPCVCNHSSSRSPWFSICKGLEWFQRHVSWKIKNGRSFSFWHSHWHQNSPLSSHY 796

BLAST of Lag0012842 vs. ExPASy TrEMBL
Match: A0A5A7T9I7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00980 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.7e-89
Identity = 222/646 (34.37%), Postives = 303/646 (46.90%), Query Frame = 0

Query: 2   VSNLNWCPISGSEATSLIQPFSELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSV 61
           + NL+WCPIS   +  L +PF+E E++  LK+   NK+PGPDG+ ++F +KSW  +K ++
Sbjct: 344 IENLDWCPISNINSELLDKPFNEAEIWLTLKSFAKNKAPGPDGYAMDFLQKSWSFMKQNI 403

Query: 62  MEVFYDFYQKDIINCNVNETYITLIPKKNTALQIQDFRSISLTTVLYRLIAKTLAERLKS 121
            ++F DF+   IIN  VNET ITLI KK       DFR ISLTT +Y+LIAKTLA+RLK 
Sbjct: 404 CDIFKDFHSTHIINKVVNETLITLIAKKEHCETAADFRPISLTTAIYKLIAKTLADRLKQ 463

Query: 122 TLQGTVSENQLAFVKGRQITDAILVANEVVDFWKCSHTRGFVIKLDIEKAFDKICWNFID 181
           TL  T+SE+Q+AFVKGRQIT+AIL+ANE +DFW+    RGFVIKLDIEKAFDK+ W FID
Sbjct: 464 TLPDTISESQMAFVKGRQITEAILIANEALDFWRSKKERGFVIKLDIEKAFDKLNWRFID 523

Query: 182 KILAFKGYPITWH----------------------------------------------- 241
            +L  K Y   W                                                
Sbjct: 524 FVLMKKNYSQKWRKMIASCISSVQYSILINGRPRGRIKPSRGIRQGDPLSPFIFVLAMDY 583

Query: 242 --------------------------------DILLFVQDNDAMLGNLFNIIKVFELSSG 301
                                           DIL+FV+D D  + NL  I+ +FE +SG
Sbjct: 584 LSRLLNNLADKRKINGVKFSPNLNLTHILFADDILIFVEDRDDYVSNLKMILHLFESASG 643

Query: 302 LNINFSKSSITGINVEDVRVAQIAANWGCPMAQFPIPYLGSPLGIRLSDLFFG---MGKI 361
           LNIN SKS+I  INV   R   IA +WG      P  YLG PLG R S   F    + KI
Sbjct: 644 LNINLSKSTIFPINVPTDRAKSIADSWGISKGHLPTSYLGMPLGGRPSSSNFWDNVLQKI 703

Query: 362 KVVASLW------------LIGA-------WWLRLLRLGVWAYSRLELQIAHF----KSN 421
           +   S W            LI +       + + + ++      ++E    +F     SN
Sbjct: 704 QKKLSNWKYSQLSKGGRITLINSTLESLPIYQMSVFKVPKGIAQKIEASWRNFLWNGASN 763

Query: 422 GFGDSFMR--RLLCGSESSQQNIQPIDKELFQL----------RRDSLPPR--------- 481
           G   S +R  +++   E     I  ++   F L           +D L  R         
Sbjct: 764 GHNISLIRWNQIVSPKEKGGLGIHSVNSTNFALLCKWLWKFLTEKDPLWKRLIISKYDKE 823

Query: 482 ---------------APWTSILKQASLFLANTVWNLKDGSKISFWHDSWTDHGPLYQAIP 506
                          +PW ++ +  S F  N  W + DG  ISFW D+W  + PL  A+P
Sbjct: 824 KMGSFPSHGKFSSNNSPWKAVTECISWFYKNISWKVNDGEDISFWLDNWNGNAPLSLAVP 883

BLAST of Lag0012842 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 8.2e-12
Identity = 36/89 (40.45%), Postives = 52/89 (58.43%), Query Frame = 0

Query: 23  SELEVFQNLKAMGHNKSPGPDGFTVEFFKKSWIDIKPSVMEVFYDFYQKDIINCNVNETY 82
           S+ E+   + AM  NK+PGPD FT EFF +SW  +K S +    +F++   +    N T 
Sbjct: 532 SDKEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATA 591

Query: 83  ITLIPKKNTALQIQDFRSISLTTVLYRLI 112
           ITLIPK     Q+  FR +S  TV+Y++I
Sbjct: 592 ITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

BLAST of Lag0012842 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 54.7 bits (130), Expect = 2.7e-07
Identity = 27/81 (33.33%), Postives = 46/81 (56.79%), Query Frame = 0

Query: 115 LAERLKSTLQGTVSENQLAFVKGRQITDAILVANEVV-DFWKCSHTRGF-VIKLDIEKAF 174
           + ERLK  +   +   Q +F+ GR  TD I+   E V    +    +G+ ++KLD+EKA+
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 175 DKICWNFIDKILAFKGYPITW 194
           D+I W++++  L   G+P  W
Sbjct: 61  DRIRWDYLEDTLISAGFPEVW 81