Lag0012802 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012802
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionTranscription termination factor MTEF1
Locationchr1: 44497415 .. 44498413 (-)
RNA-Seq ExpressionLag0012802
SyntenyLag0012802
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGACACAGCTCTCCACCTCCTCTCCAATGGCGGATTCATCCACCATTCTTCTCCTTCCCCACTTTTCCCCTCTCTTTCCCGCTCCCGAACCCTCCAATTCCCCATTCTCTCCAACGCCAAAATCACCTTCATCCTCCCCTCTAAACTCCCTCCTCGCACCCCCGACATTCCCCCTCTATCGCCGCCGCTCCCTCCGCCGCCGCCCCACCGCGACCCCCGCTCCGAGTTTCAGGAGAAAATGCTCTTCCTCGACTCCATCGGCATCGACTTCCTCTCCCTCATCAAAGACCATCCTCCCGTCGCCGCCGCCTCCCTCGCCGACCTCAGATCCGCCGTCGATTTCATGACCTCCATGAACTTCACCGCCCTCGAGTTCCGGAGAATCGTCGGTATGTGCCCTGAGATTCTCACCTCTCGCGCCTCCGATATCGTCCCGGTCTTCACCTTCCTCCTCCGCGAGGCTCGTGTCGACGGCTCCGACATCAAGCGCGTCATCAATCGACGCCCTAGGTTGCTCGCCTGTAGCGTGAAGAATCGGCTCCGTCCGACTCTGTACTTCCTGCAAAGCATCGGCATAACCGAGGTACATAAACACACTTCCTTACTCTCCTGTAGCGTCGAAGAGAAGTTGATTCCGAGGATCGAGTTCTTCGAGAACCTAGGGTTTTGTCGCCGGGACGCCGTGATAATGTTCCGGCGATTCCCGCAGCTGTTCTGCTACAGCATCAAGGAGAATCTGGAGCCGAAACTGAATTACTTCGTGGTGGAAATGGGTAGAGAATTGAAGGAATTGAAGGAGTTTCCTCATTACTTTTCGTTTAGCTTAGAGAATAGGATCAAGCCGAGACATCAAAGCTGTGTGGAAAAGGGAGTTTGTTTTCCATTGCCGGTGTTGTTAAAAACCAGTGAGATGAAATTCCGGGAAAAATTGGAGGTATGTTGTGATTCTTCCATACCCTTTAGAACTTCTCCTTTGTGGTGTACAAAATTGTGA

mRNA sequence

ATGCAGGACACAGCTCTCCACCTCCTCTCCAATGGCGGATTCATCCACCATTCTTCTCCTTCCCCACTTTTCCCCTCTCTTTCCCGCTCCCGAACCCTCCAATTCCCCATTCTCTCCAACGCCAAAATCACCTTCATCCTCCCCTCTAAACTCCCTCCTCGCACCCCCGACATTCCCCCTCTATCGCCGCCGCTCCCTCCGCCGCCGCCCCACCGCGACCCCCGCTCCGAGTTTCAGGAGAAAATGCTCTTCCTCGACTCCATCGGCATCGACTTCCTCTCCCTCATCAAAGACCATCCTCCCGTCGCCGCCGCCTCCCTCGCCGACCTCAGATCCGCCGTCGATTTCATGACCTCCATGAACTTCACCGCCCTCGAGTTCCGGAGAATCGTCGGTATGTGCCCTGAGATTCTCACCTCTCGCGCCTCCGATATCGTCCCGGTCTTCACCTTCCTCCTCCGCGAGGCTCGTGTCGACGGCTCCGACATCAAGCGCGTCATCAATCGACGCCCTAGGTTGCTCGCCTGTAGCGTGAAGAATCGGCTCCGTCCGACTCTGTACTTCCTGCAAAGCATCGGCATAACCGAGGTACATAAACACACTTCCTTACTCTCCTGTAGCGTCGAAGAGAAGTTGATTCCGAGGATCGAGTTCTTCGAGAACCTAGGGTTTTGTCGCCGGGACGCCGTGATAATGTTCCGGCGATTCCCGCAGCTGTTCTGCTACAGCATCAAGGAGAATCTGGAGCCGAAACTGAATTACTTCGTGGTGGAAATGGGTAGAGAATTGAAGGAATTGAAGGAGTTTCCTCATTACTTTTCGTTTAGCTTAGAGAATAGGATCAAGCCGAGACATCAAAGCTGTGTGGAAAAGGGAGTTTGTTTTCCATTGCCGGTGTTGTTAAAAACCAGTGAGATGAAATTCCGGGAAAAATTGGAGGTATGTTGTGATTCTTCCATACCCTTTAGAACTTCTCCTTTGTGGTGTACAAAATTGTGA

Coding sequence (CDS)

ATGCAGGACACAGCTCTCCACCTCCTCTCCAATGGCGGATTCATCCACCATTCTTCTCCTTCCCCACTTTTCCCCTCTCTTTCCCGCTCCCGAACCCTCCAATTCCCCATTCTCTCCAACGCCAAAATCACCTTCATCCTCCCCTCTAAACTCCCTCCTCGCACCCCCGACATTCCCCCTCTATCGCCGCCGCTCCCTCCGCCGCCGCCCCACCGCGACCCCCGCTCCGAGTTTCAGGAGAAAATGCTCTTCCTCGACTCCATCGGCATCGACTTCCTCTCCCTCATCAAAGACCATCCTCCCGTCGCCGCCGCCTCCCTCGCCGACCTCAGATCCGCCGTCGATTTCATGACCTCCATGAACTTCACCGCCCTCGAGTTCCGGAGAATCGTCGGTATGTGCCCTGAGATTCTCACCTCTCGCGCCTCCGATATCGTCCCGGTCTTCACCTTCCTCCTCCGCGAGGCTCGTGTCGACGGCTCCGACATCAAGCGCGTCATCAATCGACGCCCTAGGTTGCTCGCCTGTAGCGTGAAGAATCGGCTCCGTCCGACTCTGTACTTCCTGCAAAGCATCGGCATAACCGAGGTACATAAACACACTTCCTTACTCTCCTGTAGCGTCGAAGAGAAGTTGATTCCGAGGATCGAGTTCTTCGAGAACCTAGGGTTTTGTCGCCGGGACGCCGTGATAATGTTCCGGCGATTCCCGCAGCTGTTCTGCTACAGCATCAAGGAGAATCTGGAGCCGAAACTGAATTACTTCGTGGTGGAAATGGGTAGAGAATTGAAGGAATTGAAGGAGTTTCCTCATTACTTTTCGTTTAGCTTAGAGAATAGGATCAAGCCGAGACATCAAAGCTGTGTGGAAAAGGGAGTTTGTTTTCCATTGCCGGTGTTGTTAAAAACCAGTGAGATGAAATTCCGGGAAAAATTGGAGGTATGTTGTGATTCTTCCATACCCTTTAGAACTTCTCCTTTGTGGTGTACAAAATTGTGA

Protein sequence

MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPPLSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSMNFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREKLEVCCDSSIPFRTSPLWCTKL
Homology
BLAST of Lag0012802 vs. NCBI nr
Match: KAA0025223.1 (transcription termination factor MTEF1 [Cucumis melo var. makuwa] >TYK07443.1 transcription termination factor MTEF1 [Cucumis melo var. makuwa])

HSP 1 Score: 609.4 bits (1570), Expect = 1.9e-170
Identity = 297/332 (89.46%), Postives = 316/332 (95.18%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           M D++LHLLSNGGFI HSSPSPLFPSL R RTL FP+LSN++ +FI PSKLPPR P IPP
Sbjct: 45  MADSSLHLLSNGGFIRHSSPSPLFPSLPRFRTLHFPVLSNSRTSFIFPSKLPPRVPTIPP 104

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LS PLPP PPH D  S+FQEKMLFLDSIGIDFLS+IKDHPPVA+ASL D+RSAVDFMTSM
Sbjct: 105 LSSPLPPSPPHTDHHSDFQEKMLFLDSIGIDFLSVIKDHPPVASASLPDIRSAVDFMTSM 164

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTALEFRRIVGMCPE+LTSRASD++P+FTFLLREARVDGSDIKRVINRRPRLLACSVK+
Sbjct: 165 NFTALEFRRIVGMCPEVLTSRASDLIPIFTFLLREARVDGSDIKRVINRRPRLLACSVKD 224

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           +LRPTLYFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDA+IMFRRFPQLF
Sbjct: 225 QLRPTLYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDALIMFRRFPQLF 284

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLP L
Sbjct: 285 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPEL 344

Query: 301 LKTSEMKFREKLEVCCDSSIPFRTSPLWCTKL 333
           LK+SEMKFREKLEVCCDSSIPFRTSPLWCTKL
Sbjct: 345 LKSSEMKFREKLEVCCDSSIPFRTSPLWCTKL 376

BLAST of Lag0012802 vs. NCBI nr
Match: XP_022972588.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 581.6 bits (1498), Expect = 4.2e-162
Identity = 292/313 (93.29%), Postives = 299/313 (95.53%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFPI+SNAK  FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPIISNAKTAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLE 314
           LKTSEMKFREKLE
Sbjct: 304 LKTSEMKFREKLE 316

BLAST of Lag0012802 vs. NCBI nr
Match: KAG7027174.1 (Transcription factor E2FA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.1 bits (1494), Expect = 1.2e-161
Identity = 291/314 (92.68%), Postives = 299/314 (95.22%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFP +SNAK  FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPTISNAKTAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLEV 315
           LKTSEMKFREKLE+
Sbjct: 304 LKTSEMKFREKLEL 317

BLAST of Lag0012802 vs. NCBI nr
Match: XP_022963280.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 575.5 bits (1482), Expect = 3.0e-160
Identity = 291/313 (92.97%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFP + NAKI FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPTI-NAKIAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLE 314
           LKTSEMKFREKLE
Sbjct: 304 LKTSEMKFREKLE 315

BLAST of Lag0012802 vs. NCBI nr
Match: XP_023518576.1 (transcription termination factor MTEF1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.5 bits (1482), Expect = 3.0e-160
Identity = 291/313 (92.97%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFP + NAKI FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPTI-NAKIAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLE 314
           LKTSEMKFREKLE
Sbjct: 304 LKTSEMKFREKLE 315

BLAST of Lag0012802 vs. ExPASy Swiss-Prot
Match: Q84X53 (Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF1 PE=2 SV=2)

HSP 1 Score: 170.6 bits (431), Expect = 2.9e-41
Identity = 96/241 (39.83%), Postives = 143/241 (59.34%), Query Frame = 0

Query: 78  FQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSMNFTALEFRRIVGMCPEI 137
           F+EK+++L  + +D    ++ +P + +A ++ + S    ++S   +     RI+ M P++
Sbjct: 33  FREKLIYLQDLNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRILDMFPDL 92

Query: 138 LTS-RASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQSIGI-- 197
           LTS   S+I+PV  FL  E  +   DI + I+R PRLL  SV  +LRP L FL+++G   
Sbjct: 93  LTSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLKTLGFVG 152

Query: 198 --TEVHKHTSLLSCSVEEKLIPRIEFF-ENLGFCRRDAVIMFRRFPQLFCYSIKENLEPK 257
             T   ++T LL  +VE  LIP+IE+  E LGF R +   M  R P L  YS+  NL PK
Sbjct: 153 RDTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVDNNLVPK 212

Query: 258 LNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREK 313
           + +F+ EM  ++KELK FP YFSFSLE +IKPRH+   E G+  PL  +LK S+ +F   
Sbjct: 213 VEFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSDGQFNHW 272

BLAST of Lag0012802 vs. ExPASy Swiss-Prot
Match: F4JVI3 (Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF5 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.7e-23
Identity = 70/234 (29.91%), Postives = 120/234 (51.28%), Query Frame = 0

Query: 84  FLDSIGI---DFLSLIKDHPPVAAASLAD-LRSAVDFMTSMNFTALEFRRIVGMCPEILT 143
           FL  +GI   D  +++   P +   SL D L+  + F+ ++     ++ +I+   P ILT
Sbjct: 238 FLLDLGIPKSDIPTILCKRPQICGISLTDNLKPTMAFLETLGIDKNQWAKIISRFPAILT 297

Query: 144 SRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQSIGI---TE 203
                +     FL +    +   I R++ R P +++ SV+++LRPT+ + +S+ +     
Sbjct: 298 YSRQKLTSTVEFLSQTGLTE-EQIGRILTRCPNIMSYSVEDKLRPTMEYFRSLNVDVAVL 357

Query: 204 VHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKENLEPKLNYFV 263
           +H+       S+E  L P  EFF   GF   +  IM  R+  L+ +S+KEN+ PK +YF 
Sbjct: 358 LHRCPQTFGLSIESNLKPVTEFFLEKGFGLDEIGIMISRYGALYTFSLKENVMPKWDYFQ 417

Query: 264 VEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFRE 311
             M     EL +FP +F +SL+ RIKPR++     GV   L  +L  S ++F +
Sbjct: 418 T-MDYPKSELVKFPQFFGYSLQERIKPRYELVQRSGVRLLLNQVLSLSGIEFEK 469

BLAST of Lag0012802 vs. ExPASy Swiss-Prot
Match: Q9FM80 (Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF9 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-21
Identity = 69/262 (26.34%), Postives = 129/262 (49.24%), Query Frame = 0

Query: 79  QEKMLFLDSIGI---DFLSLIKDHPPVAAASLA-DLRSAVDFMTSMNFTALEFRRIVGMC 138
           QE++ +L S+G+   D   ++   P +   ++  +L++ + F+  +     +  +IV   
Sbjct: 220 QERLDYLLSVGVKHRDIKRMLLRQPQILQYTVENNLKAHISFLMGLGIPNSKIGQIVAAT 279

Query: 139 PEILT-SRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFL-QSI 198
           P + + S  + + P   +L+ E  +  +D+ +V+   P++L   +         FL + +
Sbjct: 280 PSLFSYSVENSLRPTIRYLIEEVGIKETDVGKVVQLSPQILVQRLDITWNTRYMFLSKEL 339

Query: 199 G------ITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKE 258
           G      +  V KH  LL  S+++  +PRI F  ++G C  D + +     Q+   S+++
Sbjct: 340 GAPRDSVVKMVKKHPQLLHYSIDDGFLPRINFLRSIGMCNSDILKVLTSLTQVLSLSLED 399

Query: 259 NLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVE-----KGVCFPLPVLL 318
           NL+PK  Y V E+  E+  L ++P Y S SL+ RI+PRH+  VE     KG  FPL  L+
Sbjct: 400 NLKPKYMYLVNELNNEVHILTKYPMYLSLSLDQRIRPRHRFLVELKKVRKGP-FPLSSLV 459

Query: 319 KTSEMKFREKLEVCCDSSIPFR 324
              E   ++      D+ + FR
Sbjct: 460 PNDESFCQQWAGTSVDTYLAFR 480

BLAST of Lag0012802 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-16
Identity = 59/175 (33.71%), Postives = 91/175 (52.00%), Query Frame = 0

Query: 145 IVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQS-IGITE------V 204
           I P   F L+E  +    I  ++ + P LL  S+  ++RP + FL +  G+T+      +
Sbjct: 424 IAPKVRF-LQEMGIPNEAIGNMLVKFPSLLTNSLYKKIRPVVIFLLTRAGVTQKDIGKVI 483

Query: 205 HKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKENLEPKLNYFVV 264
               +LL CS+  KL P + ++ +LG        M   FP L  Y++ +NL PK  Y   
Sbjct: 484 AMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGEMIADFPMLLRYNV-DNLRPKYRYLRR 543

Query: 265 EMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREKL 313
            M R L++L EFP +FS+SLE RI PRH   VE  V F L  +L  ++ +F  ++
Sbjct: 544 TMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENRVNFKLRYMLACTDEEFERRV 596

BLAST of Lag0012802 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.1e-15
Identity = 57/227 (25.11%), Postives = 108/227 (47.58%), Query Frame = 0

Query: 94  SLIKDHPPVAAASLAD-LRSAVDFMTSMNFTALEFRRIVGMCPEILT-SRASDIVPVFTF 153
           S I   PP+ + S+ + L   + F  ++     +  +++   P +++ S  + +  + +F
Sbjct: 97  SAITKFPPILSHSVEEKLCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSF 156

Query: 154 LLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQ-SIGITE------VHKHTSLL 213
           L          I +V+ + P L+  SV  RLRPT  FL+ S+G++E      V     LL
Sbjct: 157 LASLGLDQDGMIGKVLVKNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLL 216

Query: 214 SCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKENLEPKLNYFVVEMGRELK 273
              V + L P  ++ +  GF       M   +PQ+   S+K +L+P++ + V  MGR + 
Sbjct: 217 CRDVNKILKPNYDYLKECGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMD 276

Query: 274 ELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREK 312
           E+  +P +F   L+ +++ R +   +  +   L  +L  +  KF EK
Sbjct: 277 EVASYPEFFHHGLKKKVESRFKLVKKNNIDCSLREMLDCNTKKFHEK 323

BLAST of Lag0012802 vs. ExPASy TrEMBL
Match: A0A5A7SKM3 (Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001730 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 9.1e-171
Identity = 297/332 (89.46%), Postives = 316/332 (95.18%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           M D++LHLLSNGGFI HSSPSPLFPSL R RTL FP+LSN++ +FI PSKLPPR P IPP
Sbjct: 45  MADSSLHLLSNGGFIRHSSPSPLFPSLPRFRTLHFPVLSNSRTSFIFPSKLPPRVPTIPP 104

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LS PLPP PPH D  S+FQEKMLFLDSIGIDFLS+IKDHPPVA+ASL D+RSAVDFMTSM
Sbjct: 105 LSSPLPPSPPHTDHHSDFQEKMLFLDSIGIDFLSVIKDHPPVASASLPDIRSAVDFMTSM 164

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTALEFRRIVGMCPE+LTSRASD++P+FTFLLREARVDGSDIKRVINRRPRLLACSVK+
Sbjct: 165 NFTALEFRRIVGMCPEVLTSRASDLIPIFTFLLREARVDGSDIKRVINRRPRLLACSVKD 224

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           +LRPTLYFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDA+IMFRRFPQLF
Sbjct: 225 QLRPTLYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDALIMFRRFPQLF 284

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLP L
Sbjct: 285 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPEL 344

Query: 301 LKTSEMKFREKLEVCCDSSIPFRTSPLWCTKL 333
           LK+SEMKFREKLEVCCDSSIPFRTSPLWCTKL
Sbjct: 345 LKSSEMKFREKLEVCCDSSIPFRTSPLWCTKL 376

BLAST of Lag0012802 vs. ExPASy TrEMBL
Match: A0A6J1IAF5 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111471129 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 292/313 (93.29%), Postives = 299/313 (95.53%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFPI+SNAK  FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPIISNAKTAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLE 314
           LKTSEMKFREKLE
Sbjct: 304 LKTSEMKFREKLE 316

BLAST of Lag0012802 vs. ExPASy TrEMBL
Match: A0A6J1HHJ6 (transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111463541 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.5e-160
Identity = 291/313 (92.97%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           MQDTALHLLSNGGF+HHSSPS LFPSLSRSRTLQFP + NAKI FILPSKLP R PDIPP
Sbjct: 4   MQDTALHLLSNGGFLHHSSPSSLFPSLSRSRTLQFPTI-NAKIAFILPSKLPSRAPDIPP 63

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPPPP H   RSEFQEKMLFLDSIGIDFLS+IKDHPPVA ASLAD+RSAVDFMTSM
Sbjct: 64  LSPPLPPPPSHSGSRSEFQEKMLFLDSIGIDFLSVIKDHPPVATASLADIRSAVDFMTSM 123

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTA+EFRRIVGMCPEILTSR SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN
Sbjct: 124 NFTAVEFRRIVGMCPEILTSRVSDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 183

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           RLRPT+YFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGF RRDAVIMFRRFPQLF
Sbjct: 184 RLRPTMYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFSRRDAVIMFRRFPQLF 243

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           C SIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQ CVEKGVCFPLPVL
Sbjct: 244 CCSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQICVEKGVCFPLPVL 303

Query: 301 LKTSEMKFREKLE 314
           LKTSEMKFREKLE
Sbjct: 304 LKTSEMKFREKLE 315

BLAST of Lag0012802 vs. ExPASy TrEMBL
Match: A0A1S4E3U8 (transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC107991918 PE=3 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 1.2e-157
Identity = 278/313 (88.82%), Postives = 297/313 (94.89%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           M D++LHLLSNGGFI HSSPSPLFPSL R RTL FP+LSN++ +FI PSKLPPR P IPP
Sbjct: 57  MADSSLHLLSNGGFIRHSSPSPLFPSLPRFRTLHFPVLSNSRTSFIFPSKLPPRVPTIPP 116

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LS PLPP PPH D  S+FQEKMLFLDSIGIDFLS+IKDHPPVA+ASL D+RSAVDFMTSM
Sbjct: 117 LSSPLPPSPPHTDHHSDFQEKMLFLDSIGIDFLSVIKDHPPVASASLPDIRSAVDFMTSM 176

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFTALEFRRIVGMCPE+LTSRASD++P+FTFLLREARVDGSDIKRVINRRPRLLACSVK+
Sbjct: 177 NFTALEFRRIVGMCPEVLTSRASDLIPIFTFLLREARVDGSDIKRVINRRPRLLACSVKD 236

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           +LRPTLYFLQSIGI+EVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDA+IMFRRFPQLF
Sbjct: 237 QLRPTLYFLQSIGISEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDALIMFRRFPQLF 296

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLP L
Sbjct: 297 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPEL 356

Query: 301 LKTSEMKFREKLE 314
           LK+SEMKFREKLE
Sbjct: 357 LKSSEMKFREKLE 369

BLAST of Lag0012802 vs. ExPASy TrEMBL
Match: A0A0A0KKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454440 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 2.3e-126
Identity = 238/314 (75.80%), Postives = 256/314 (81.53%), Query Frame = 0

Query: 1   MQDTALHLLSNGGFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIPP 60
           M D++LHLLSNGGFIHHSSPSPLFPSL RSRTL FP+LSN++ +FI PSKLPPR P IPP
Sbjct: 1   MADSSLHLLSNGGFIHHSSPSPLFPSLPRSRTLHFPVLSNSRTSFIFPSKLPPRNPAIPP 60

Query: 61  LSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSM 120
           LSPPLPP PPH D  S+FQEKMLFLDSIGIDFLS+IKDHPPVA+ASL D+RSAVDFMTSM
Sbjct: 61  LSPPLPPSPPHTDHHSDFQEKMLFLDSIGIDFLSVIKDHPPVASASLPDIRSAVDFMTSM 120

Query: 121 NFTALEFRRIVGMCPEILTSRASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKN 180
           NFT LE RRIVGMCPEILTSRAS ++P+FTFLLREARVDGSDIKRVINRRPRLLACSVK+
Sbjct: 121 NFTTLELRRIVGMCPEILTSRASVLIPIFTFLLREARVDGSDIKRVINRRPRLLACSVKD 180

Query: 181 RLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLF 240
           +LRPTLYFLQSIGI+E                                          LF
Sbjct: 181 QLRPTLYFLQSIGISE------------------------------------------LF 240

Query: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVL 300
           CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLP L
Sbjct: 241 CYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPEL 272

Query: 301 LKTSEMKFREKLEV 315
           LK+SEMKFREKLE+
Sbjct: 301 LKSSEMKFREKLEL 272

BLAST of Lag0012802 vs. TAIR 10
Match: AT2G36000.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.4e-94
Identity = 188/332 (56.63%), Postives = 233/332 (70.18%), Query Frame = 0

Query: 1   MQDTALHLLSNG-GFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIP 60
           MQ  AL  LS+    +HH+     FPSLSR R   FP LS    T    S    ++PDIP
Sbjct: 1   MQQEALSFLSSSLPSLHHN-----FPSLSRLRFNNFPALSFKPNTSSSSSSF-FKSPDIP 60

Query: 61  PLSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTS 120
            LS             S   EK+++LDS+GIDFL+LI  HPP+ + +L+ + S VD+MT+
Sbjct: 61  SLSSTTTTTTTTETLESSIHEKLIYLDSLGIDFLTLINRHPPLLSTALSAVESVVDYMTT 120

Query: 121 --MNFTALEFRRIVGMCPEILTS-RASDIVPVFTFLLREARVDG-SDIKRVINRRPRLLA 180
             +NFT  +FRR+V MCPE+LTS   S  +PV TFLLRE  VD   D+++ + RRPRLLA
Sbjct: 121 PPINFTLQDFRRLVSMCPELLTSPLTSHTIPVITFLLREVGVDSIFDLRQALRRRPRLLA 180

Query: 181 CSVKNRLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRR 240
           CSV ++LRPTLYFLQ IGI + HKHT LLSCSV+ KL+PRI++FE LGF RR A  MF+R
Sbjct: 181 CSVDHQLRPTLYFLQRIGILDPHKHTYLLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKR 240

Query: 241 FPQLFCYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCF 300
           FPQLF YSI EN EPKL Y +VEMGR+++E+ EFP YFSFSLENRIKPRH++C  KGV F
Sbjct: 241 FPQLFNYSIAENYEPKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRF 300

Query: 301 PLPVLLKTSEMKFREKLEVCCDSSIPFRTSPL 328
           PLPV+LKT+E  FR+ LEVCCDSS P +TS L
Sbjct: 301 PLPVMLKTNEAGFRDTLEVCCDSSPPLKTSRL 326

BLAST of Lag0012802 vs. TAIR 10
Match: AT2G36000.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.1e-88
Identity = 179/319 (56.11%), Postives = 223/319 (69.91%), Query Frame = 0

Query: 1   MQDTALHLLSNG-GFIHHSSPSPLFPSLSRSRTLQFPILSNAKITFILPSKLPPRTPDIP 60
           MQ  AL  LS+    +HH+     FPSLSR R   FP LS    T    S    ++PDIP
Sbjct: 1   MQQEALSFLSSSLPSLHHN-----FPSLSRLRFNNFPALSFKPNTSSSSSSF-FKSPDIP 60

Query: 61  PLSPPLPPPPPHRDPRSEFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTS 120
            LS             S   EK+++LDS+GIDFL+LI  HPP+ + +L+ + S VD+MT+
Sbjct: 61  SLSSTTTTTTTTETLESSIHEKLIYLDSLGIDFLTLINRHPPLLSTALSAVESVVDYMTT 120

Query: 121 --MNFTALEFRRIVGMCPEILTS-RASDIVPVFTFLLREARVDG-SDIKRVINRRPRLLA 180
             +NFT  +FRR+V MCPE+LTS   S  +PV TFLLRE  VD   D+++ + RRPRLLA
Sbjct: 121 PPINFTLQDFRRLVSMCPELLTSPLTSHTIPVITFLLREVGVDSIFDLRQALRRRPRLLA 180

Query: 181 CSVKNRLRPTLYFLQSIGITEVHKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRR 240
           CSV ++LRPTLYFLQ IGI + HKHT LLSCSV+ KL+PRI++FE LGF RR A  MF+R
Sbjct: 181 CSVDHQLRPTLYFLQRIGILDPHKHTYLLSCSVDNKLVPRIDYFEKLGFSRRSATAMFKR 240

Query: 241 FPQLFCYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCF 300
           FPQLF YSI EN EPKL Y +VEMGR+++E+ EFP YFSFSLENRIKPRH++C  KGV F
Sbjct: 241 FPQLFNYSIAENYEPKLKYLMVEMGRDVREVLEFPQYFSFSLENRIKPRHEACAAKGVRF 300

Query: 301 PLPVLLKTSEMKFREKLEV 315
           PLPV+LKT+E  FR+ LEV
Sbjct: 301 PLPVMLKTNEAGFRDTLEV 313

BLAST of Lag0012802 vs. TAIR 10
Match: AT2G34620.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 189.5 bits (480), Expect = 4.3e-48
Identity = 98/241 (40.66%), Postives = 158/241 (65.56%), Query Frame = 0

Query: 77  EFQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSMNFTALEFRRIVGMCPE 136
           + +EK+L L+ +GID    +  +P + +A L  ++S + F+ S      +  RI+GMCP+
Sbjct: 54  QMKEKILCLELMGIDSGKALSLNPCLCSAPLDSIQSVLHFLQSKGIYPNDLPRILGMCPK 113

Query: 137 ILTSRA-SDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQSIGIT 196
           ILTS   +++ PVF FL  +  V  +  +RVI + PRLL  SV+++L+P L++LQ +G+ 
Sbjct: 114 ILTSDVRTELYPVFMFLSNDLHVPENAFRRVIKKCPRLLISSVEDQLKPALFYLQRLGLK 173

Query: 197 EV----HKHTSLLSCSVEEKLIPRIEFFENLGFCRRDAVIMFRRFPQLFCYSIKENLEPK 256
           ++    ++   LL  SVE  LIP++ F E++GF R +A+ M  R P LF +SI+ N +PK
Sbjct: 174 DLEALAYQDPILLVSSVEHTLIPKLRFLESIGFSRPEAIGMILRCPALFTFSIENNFKPK 233

Query: 257 LNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREK 313
           L+YF+ E+  +L+ LKEFP YF+FSLE RIKPRH   +E+G+  PL ++LK+++ +F + 
Sbjct: 234 LDYFMSEIKGKLENLKEFPQYFAFSLEKRIKPRHLESMERGLELPLSLMLKSTDEEFEQL 293

BLAST of Lag0012802 vs. TAIR 10
Match: AT2G03050.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.1e-42
Identity = 96/241 (39.83%), Postives = 143/241 (59.34%), Query Frame = 0

Query: 78  FQEKMLFLDSIGIDFLSLIKDHPPVAAASLADLRSAVDFMTSMNFTALEFRRIVGMCPEI 137
           F+EK+++L  + +D    ++ +P + +A ++ + S    ++S   +     RI+ M P++
Sbjct: 32  FREKLIYLQDLNVDPHKALRVNPSLRSAPISSVVSVETLLSSTGLSRPAVGRILDMFPDL 91

Query: 138 LTS-RASDIVPVFTFLLREARVDGSDIKRVINRRPRLLACSVKNRLRPTLYFLQSIGI-- 197
           LTS   S+I+PV  FL  E  +   DI + I+R PRLL  SV  +LRP L FL+++G   
Sbjct: 92  LTSDPESEILPVLRFLSNEISISEQDIPKSISRCPRLLISSVDYQLRPALTFLKTLGFVG 151

Query: 198 --TEVHKHTSLLSCSVEEKLIPRIEFF-ENLGFCRRDAVIMFRRFPQLFCYSIKENLEPK 257
             T   ++T LL  +VE  LIP+IE+  E LGF R +   M  R P L  YS+  NL PK
Sbjct: 152 RDTITSRNTVLLVSNVERTLIPKIEYLEEGLGFTREEVAKMVVRSPALLTYSVDNNLVPK 211

Query: 258 LNYFVVEMGRELKELKEFPHYFSFSLENRIKPRHQSCVEKGVCFPLPVLLKTSEMKFREK 313
           + +F+ EM  ++KELK FP YFSFSLE +IKPRH+   E G+  PL  +LK S+ +F   
Sbjct: 212 VEFFIEEMRGDVKELKRFPQYFSFSLERKIKPRHRLLKEHGILMPLSEMLKVSDGQFNHW 271

BLAST of Lag0012802 vs. TAIR 10
Match: AT3G18870.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 129.4 bits (324), Expect = 5.3e-30
Identity = 88/268 (32.84%), Postives = 143/268 (53.36%), Query Frame = 0

Query: 58  IPPLSPPLPPPPPHRDP------RSEFQEKMLFLDSIGIDFLSLIKDHPPVA--AASLAD 117
           + PLS    PP P          ++  +E +  L S+GI     +  +P +A  A  L+ 
Sbjct: 10  LQPLSSFSSPPSPSSSKPLYLKFQTSHRENLRHLSSLGI-----VPQNPRLAPPANDLSV 69

Query: 118 LRSAVDFMTSMNFTALEFRRIVGMCPEIL--TSRASDIVPVFTFLLREARVDGSDIKRVI 177
           + SAV+ + S   +  +F R+V +CP++   T   S + PVF FL  E      + + +I
Sbjct: 70  ILSAVNLLKSKGISDEDFPRLVFLCPQLFSPTFDISKLDPVFDFLTGELGASAEESRGLI 129

Query: 178 NRRPRLLACSVKNRLRPTLYFLQSIGITEVHK----HTSLLSCSVEEKLIPRIEFFENLG 237
              P +L   V+  LRPTL +L+ +G+  +++    +  +L+  V EKL  ++ F +++G
Sbjct: 130 VNCPNILFSDVEYCLRPTLVYLKELGVRNLNRASKTNAHVLNTRV-EKLRAKMRFLKSIG 189

Query: 238 FCRRDAVIMFRRFPQLFCYSIKENLEPKLNYFVVEMGRELKELKEFPHYFSFSLENRIKP 297
           F   +A  +  R P +F YS+++NL PK  + V +M REL+ELK+FP YF+FSL  RI+P
Sbjct: 190 FEHEEAARVCGRIPAIFGYSVEDNLRPKFEFLVYDMERELEELKKFPQYFAFSLGKRIRP 249

Query: 298 RHQSCVEKGVCFPLPVLLKTSEMKFREK 312
           RH    +K V   L  +L   + KF  K
Sbjct: 250 RHWHLKKKNVRVSLSRMLMWGDQKFYSK 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0025223.11.9e-17089.46transcription termination factor MTEF1 [Cucumis melo var. makuwa] >TYK07443.1 tr... [more]
XP_022972588.14.2e-16293.29transcription termination factor MTEF1, chloroplastic [Cucurbita maxima][more]
KAG7027174.11.2e-16192.68Transcription factor E2FA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022963280.13.0e-16092.97transcription termination factor MTEF1, chloroplastic [Cucurbita moschata][more]
XP_023518576.13.0e-16092.97transcription termination factor MTEF1, chloroplastic [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
Q84X532.9e-4139.83Transcription termination factor MTEF1, chloroplastic OS=Arabidopsis thaliana OX... [more]
F4JVI32.7e-2329.91Transcription termination factor MTERF5, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9FM802.6e-2126.34Transcription termination factor MTERF9, chloroplastic OS=Arabidopsis thaliana O... [more]
F4IHL31.5e-1633.71Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL66.1e-1525.11Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A5A7SKM39.1e-17189.46Transcription termination factor MTEF1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1IAF52.0e-16293.29transcription termination factor MTEF1, chloroplastic OS=Cucurbita maxima OX=366... [more]
A0A6J1HHJ61.5e-16092.97transcription termination factor MTEF1, chloroplastic OS=Cucurbita moschata OX=3... [more]
A0A1S4E3U81.2e-15788.82transcription termination factor MTEF1, chloroplastic OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KKL62.3e-12675.80Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G454440 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G36000.11.4e-9456.63Mitochondrial transcription termination factor family protein [more]
AT2G36000.21.1e-8856.11Mitochondrial transcription termination factor family protein [more]
AT2G34620.14.3e-4840.66Mitochondrial transcription termination factor family protein [more]
AT2G03050.12.1e-4239.83Mitochondrial transcription termination factor family protein [more]
AT3G18870.15.3e-3032.84Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 263..294
e-value: 0.0022
score: 27.2
coord: 128..159
e-value: 1.1
score: 18.3
coord: 196..225
e-value: 0.042
score: 23.0
coord: 93..123
e-value: 490.0
score: 6.3
coord: 230..262
e-value: 1.7E-5
score: 34.3
coord: 164..195
e-value: 1.9E-5
score: 34.1
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 154..308
e-value: 8.4E-34
score: 116.9
coord: 84..196
e-value: 3.2E-11
score: 42.7
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 67..321
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 144..315
e-value: 4.5E-37
score: 129.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 56..76
NoneNo IPR availablePANTHERPTHR13068:SF46OS03G0360600 PROTEINcoord: 67..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012802.1Lag0012802.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0032502 developmental process
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003690 double-stranded DNA binding