Lag0012747 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0012747
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	GDSL esterase/lipase
Location	chr1: 43995913 .. 43998433 (+)
RNA-Seq Expression	Lag0012747
Synteny	Lag0012747

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGCGTGTTTGCTTTCAGGGCAAGGGATTGCTGGTCATCATCACCATCATCACCACCATCGCTACCATCGCCATTTTGAACTGAGCAAGCTCTTCGTTTTTGGAGATTCATATGTCGACACTGGCAACGTCGCTCCATCTGACTCAAATTATCCAAAGTATCCATATGGAATTACCTTTCCTGGCAAACCTGATGGACGTTTCTCCAATGGTCGTGTCTTGACCGATTACGTGGGTACATGACCGAACATCCCTCTCTCTCTCTCACACAACACCCTTCACAGTTCACACACAAATGTTAATTCAGAGATTGTAGTTAAGTGTTGAATTGTAATTTGTAAATGCATGCATGCAGCGAGAGTTGTAGGGCTGAAATCTCCAATCCCATATAGACATTTGAACAAGGTAGGACTGCAAGGGGCAAAAGCTGGCATCAACTTCGCATATGGTGGAACTGGTGTGTTCAAGACAGGCTATGATCTTCCAACCATGACCACCCAGATTCACTTTCTACAACACCTTATTGCCAACTCTTCATTTACGCCAGACGATATTAAATCCTCCTTTGCCCTTGTCTCTGTCTCTGGCAATGACTATTCTTATTACCTATCCACAAATGGCTCCATTCAGGTGACCTACACTCTCTTTCGTTCACCTAAAATATATAACTCATCTCTTTCCTTCATGTCATGCATAGTGGAAATTTTCATTTATGCTTTACTTAATTTATCGAGTCGAATTTAATTTTATTCTACGGAGAAATTATTTGAGGAGGTTGTGAAAACGTGACTGACAGTTTTGCATTATTGTTGGTTGCAAATAATATATGGAAGTGAAATTGAAAAACAATACTTCGGATTTGTATATAGTGAGTTATTACGAAGAAGTATGCAAAATTTTGAATCCAAATCCAACTGTTAACATTTGGTTATTTCTCTTTTGTTGTTTTCTCTTTCCCACTGAGCTCTTTCTATTTTCTTTCTCTTCTTCTTCCTTAACTTTCTCTTATTCTCCATTATCCTCACCTCTTCTCTCTCAAACTTTCTCTTCTTCTCAATTGTTCTCTCTCCTTCTCCCTCAACTTTCTTTTTTCTCCCTCAACCTTCTATCTTCTCTATTTTTCTTTCATTCTTCTCCCTCACAACTACCAAAATTGATCGACCGAACGTTTCACAAAGCACCCTAAAACACGATCGGTCAGTTTTATGAAGCACCCTAAATTTAAACCGTGCGCCACCAATGGACCAAAATTGATAAGTCAGTTTCCGACGTTGATTTTGGACAAAAATCAACGTCATGAGTAGTCCTATGAATGAGAGACAATATCTCAATTGGTAGGAGATCTTTCAGATAAAATCGTCACAAGGGCTTATACCAAAAATTGACTATATCATACAATTGTCGAGATATGTAGAGGCTTTGTGTTGTCTCTCACAAATACCTTCATTAATTGAGTTATGATTGTTTTTCAACTCAACGATATGAAGACGAGTAATAATTACGCTCACGTTGTCTACAATTTTTATGTTTAAACTTTTATTATTTAGTTGGGTATATATAGTTATTGTATGGTTGAGCTAGTTTGACCAGGCTTTTTTTATAATAATTTTCACAGTTCATTAAAATGAAAAATAAAAAATGATTACTGTTTAACCAAAATGCACATATATAAATGAATTTCCTCCCTATAAACATGGTAATCTAGCTAGGTAAGTTGTAATAAAATTGACATATAATATTTATTATTTACTTATATATTAATTTCATGTGTTTTATGTGGTTTTAGGGTTTCATACCACTGATTCAGAGAGTAGTGAATCAAATCAGTGTGAACTTGAAACGCATCCACAGCTTTGGAGTGAAGAAAATAATTGTAACAGCATTAGGGCCATTGCAATGTGTGCCCGACATTACAGCCCTTTCTGGTTTCAAGCAGTGCAATTCAACTTTAACTCCATTGGTCAACCTCCACAACTTGCTGTTAACTCAAGCTGTCGACAAATTGAACAACCAAACAAATAATGATTCTCCTTTCTTCATTCTCAATCTCTATAGTGCTTTTTTATCCATAATCCAAAACAAAGGAGACCCTCAAGGTGAAGAACAAAAACGACGGGTTTTCTAAGACTCCGTTTGATAATTAACCATTTGATTTTTACGTAAGTTTAAAAACGTTTTTTTTTAGAAAATGAGACGAAAAACAGAAAAGACATCAAACGCGTTTGTTTCTCTATAGAAACAAGAAACACGAAATAAAAAATAAGAACAGAAAACCCTAATATCTTTTTTCTGTCTTATGCACGCAGGAAACATAACATTTTCAACCGCAATGCAGCCATGTTGCATTGGAATAAGCCCTGAATATAATTGTGGAAGTGTAGACGAACATGGCAACAAGAAGTATGTATTGTGCGATGCTCCAAATTCATCCTTCTATTGGGACGGAGTTCATCCTACACAAGAGGGATGGATTGCAGCGCTTACCACCTTGATCTCCACTATGAAACAACATTTCTAG

mRNA sequence

ATGTGTGCGTGTTTGCTTTCAGGGCAAGGGATTGCTGGTCATCATCACCATCATCACCACCATCGCTACCATCGCCATTTTGAACTGAGCAAGCTCTTCGTTTTTGGAGATTCATATGTCGACACTGGCAACGTCGCTCCATCTGACTCAAATTATCCAAAGTATCCATATGGAATTACCTTTCCTGGCAAACCTGATGGACGTTTCTCCAATGGTCGTGTCTTGACCGATTACGTGGCGAGAGTTGTAGGGCTGAAATCTCCAATCCCATATAGACATTTGAACAAGGTAGGACTGCAAGGGGCAAAAGCTGGCATCAACTTCGCATATGGTGGAACTGGTGTGTTCAAGACAGGCTATGATCTTCCAACCATGACCACCCAGATTCACTTTCTACAACACCTTATTGCCAACTCTTCATTTACGCCAGACGATATTAAATCCTCCTTTGCCCTTGTCTCTGTCTCTGGCAATGACTATTCTTATTACCTATCCACAAATGGCTCCATTCAGGGTTTCATACCACTGATTCAGAGAGTAGTGAATCAAATCAGTGTGAACTTGAAACGCATCCACAGCTTTGGAGTGAAGAAAATAATTGTAACAGCATTAGGGCCATTGCAATGTGTGCCCGACATTACAGCCCTTTCTGGTTTCAAGCAGTGCAATTCAACTTTAACTCCATTGGTCAACCTCCACAACTTGCTGTTAACTCAAGCTGTCGACAAATTGAACAACCAAACAAATAATGATTCTCCTTTCTTCATTCTCAATCTCTATAGTGCTTTTTTATCCATAATCCAAAACAAAGGAGACCCTCAAGGAAACATAACATTTTCAACCGCAATGCAGCCATGTTGCATTGGAATAAGCCCTGAATATAATTGTGGAAGTGTAGACGAACATGGCAACAAGAAGTATGTATTGTGCGATGCTCCAAATTCATCCTTCTATTGGGACGGAGTTCATCCTACACAAGAGGGATGGATTGCAGCGCTTACCACCTTGATCTCCACTATGAAACAACATTTCTAG

Coding sequence (CDS)

ATGTGTGCGTGTTTGCTTTCAGGGCAAGGGATTGCTGGTCATCATCACCATCATCACCACCATCGCTACCATCGCCATTTTGAACTGAGCAAGCTCTTCGTTTTTGGAGATTCATATGTCGACACTGGCAACGTCGCTCCATCTGACTCAAATTATCCAAAGTATCCATATGGAATTACCTTTCCTGGCAAACCTGATGGACGTTTCTCCAATGGTCGTGTCTTGACCGATTACGTGGCGAGAGTTGTAGGGCTGAAATCTCCAATCCCATATAGACATTTGAACAAGGTAGGACTGCAAGGGGCAAAAGCTGGCATCAACTTCGCATATGGTGGAACTGGTGTGTTCAAGACAGGCTATGATCTTCCAACCATGACCACCCAGATTCACTTTCTACAACACCTTATTGCCAACTCTTCATTTACGCCAGACGATATTAAATCCTCCTTTGCCCTTGTCTCTGTCTCTGGCAATGACTATTCTTATTACCTATCCACAAATGGCTCCATTCAGGGTTTCATACCACTGATTCAGAGAGTAGTGAATCAAATCAGTGTGAACTTGAAACGCATCCACAGCTTTGGAGTGAAGAAAATAATTGTAACAGCATTAGGGCCATTGCAATGTGTGCCCGACATTACAGCCCTTTCTGGTTTCAAGCAGTGCAATTCAACTTTAACTCCATTGGTCAACCTCCACAACTTGCTGTTAACTCAAGCTGTCGACAAATTGAACAACCAAACAAATAATGATTCTCCTTTCTTCATTCTCAATCTCTATAGTGCTTTTTTATCCATAATCCAAAACAAAGGAGACCCTCAAGGAAACATAACATTTTCAACCGCAATGCAGCCATGTTGCATTGGAATAAGCCCTGAATATAATTGTGGAAGTGTAGACGAACATGGCAACAAGAAGTATGTATTGTGCGATGCTCCAAATTCATCCTTCTATTGGGACGGAGTTCATCCTACACAAGAGGGATGGATTGCAGCGCTTACCACCTTGATCTCCACTATGAAACAACATTTCTAG

Protein sequence

MCACLLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPTMTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF

Homology

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BLAST of Lag0012747 vs. NCBI nr
Match: XP_038883064.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 578.9 bits (1491), Expect = 2.8e-161
Identity = 273/340 (80.29%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLS +G AGHH HHHH   H HF+ SKLFVFGDSYVDTGN++PSDSNYPKYPYGITFPGK
Sbjct: 16  LLSEKGFAGHHLHHHH--LHHHFQPSKLFVFGDSYVDTGNISPSDSNYPKYPYGITFPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           PDGRFSNGRVLTD+ AR +GLKSPIPYRHL K+G++GAK G+NFAYGGTGVFKTGYDLPT
Sbjct: 76  PDGRFSNGRVLTDFAARFIGLKSPIPYRHLEKIGIEGAKYGVNFAYGGTGVFKTGYDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQ+LIA+S+FTP+ I SSFALVSVSGNDYSYYLSTNGSIQGFIPLI++VV QI
Sbjct: 136 MTTQIDFLQNLIADSTFTPNQISSSFALVSVSGNDYSYYLSTNGSIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
            VNLKRIHSFGVKKI +TALGPLQCVP+IT +S FK+CNST++ LV+ HN LL QAVDKL
Sbjct: 196 RVNLKRIHSFGVKKISITALGPLQCVPEITVVSDFKECNSTISALVDFHNFLLQQAVDKL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T NDSPFF+LNL+SAF SIIQNKG PQ NITF+T +QPCCIGI+PEY+CGSVDEHGN
Sbjct: 256 NKET-NDSPFFVLNLHSAFSSIIQNKGVPQENITFATPLQPCCIGINPEYDCGSVDEHGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WD VHPTQ+GWIAAL+ LISTMKQHF
Sbjct: 316 KKYVLCDDPNSAFFWDAVHPTQQGWIAALSILISTMKQHF 352

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BLAST of Lag0012747 vs. NCBI nr
Match: XP_008462554.1 (PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo] >KAA0025272.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07396.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 560.1 bits (1442), Expect = 1.4e-155
Identity = 264/340 (77.65%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG+G A HHHHH       HF+ SKLFVFGDSYVDTGNV+PSDSNYPKYPYGIT+PGK
Sbjct: 16  LLSGKGFASHHHHH-----RSHFQPSKLFVFGDSYVDTGNVSPSDSNYPKYPYGITYPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           P GRFS+GRVLTD+ A ++GLKSPIPYRHL KVGL+G K G+NFAYGGTGVFKTG+DLPT
Sbjct: 76  PAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTGVFKTGFDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQ LIANS+FTPD IKSSFALVSVSGNDYSYYLSTNG IQGFIPLI++VV QI
Sbjct: 136 MTTQIDFLQTLIANSTFTPDQIKSSFALVSVSGNDYSYYLSTNGPIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
           SVNL+RIHSFGVKKI +TALGPLQCVP+ITAL+ FK+CNSTL+ LV+ HN LLT+AVD+L
Sbjct: 196 SVNLRRIHSFGVKKISITALGPLQCVPEITALTDFKECNSTLSQLVDFHNFLLTRAVDEL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND PFFILNL+ AF S+IQNKG+PQGNI F   ++PCCIGI+PEYNCGSVD+ GN
Sbjct: 256 NKET-NDYPFFILNLHDAFSSVIQNKGNPQGNIKFENPLKPCCIGINPEYNCGSVDKDGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WDGVHPTQ+GW AALT L+S  KQHF
Sbjct: 316 KKYVLCDDPNSAFFWDGVHPTQQGWFAALTILLSKFKQHF 349

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BLAST of Lag0012747 vs. NCBI nr
Match: XP_004143312.1 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48249.1 hypothetical protein Csa_003600 [Cucumis sativus])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 259/340 (76.18%), Postives = 294/340 (86.47%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG+G  GHHHHHHH   H HF+ +KLFVFGDSYVDTGNV+PSDSNYP YPYGIT+PGK
Sbjct: 16  LLSGKGFGGHHHHHHH--VHSHFKPTKLFVFGDSYVDTGNVSPSDSNYPTYPYGITYPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           P GRFS+GRVL+D+ A ++G KSPIPYRHL KVG++G K G+NFAYGGTGVFKTGYDLPT
Sbjct: 76  PAGRFSDGRVLSDFAANLIGQKSPIPYRHLEKVGIKGTKYGVNFAYGGTGVFKTGYDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MT+QI FL  LIANS+FTP  I SSFALVSVSGNDYSYYLS NG IQGFIPLI++VV QI
Sbjct: 136 MTSQIDFLHTLIANSTFTPSQINSSFALVSVSGNDYSYYLSQNGPIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
           SVNLKRIHSFGVKKI +TALGPL CVP++T L+ FK+CNSTL+ LV+ HN LL QAVD+L
Sbjct: 196 SVNLKRIHSFGVKKIGITALGPLHCVPEVTVLTDFKECNSTLSQLVDFHNHLLKQAVDEL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND PFFILNL+ AFLSIIQNKG PQGNI F T ++PCCIGI+P+YNCGSVD+ GN
Sbjct: 256 NKET-NDLPFFILNLHDAFLSIIQNKGIPQGNIKFETPLKPCCIGINPQYNCGSVDKDGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WDGVHPTQ GWIAALT L+S  KQHF
Sbjct: 316 KKYVLCDDPNSAFFWDGVHPTQRGWIAALTILLSNFKQHF 352

[Close]

BLAST of Lag0012747 vs. NCBI nr
Match: XP_023544735.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.0 bits (1403), Expect = 4.5e-151
Identity = 262/339 (77.29%), Postives = 292/339 (86.14%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG G+A HHHH      HRH + SKLFVFGDSYVDTGN +PSD NYPK PYG+TFPG 
Sbjct: 15  LLSGLGLARHHHHQ-----HRHLQTSKLFVFGDSYVDTGNTSPSDPNYPKTPYGVTFPGH 74

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           PDGRFSNGRVLTDYVAR++GLKSPIPYR + K G++GAK G+NFAYGGTGVFKTGYDLPT
Sbjct: 75  PDGRFSNGRVLTDYVARIIGLKSPIPYRVVEKEGVEGAKDGVNFAYGGTGVFKTGYDLPT 134

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQ+LIANS+FTP DI SSFALVSVSGNDYSYYLSTNGSI+GF+PLI RVV QI
Sbjct: 135 MTTQIDFLQYLIANSTFTPQDINSSFALVSVSGNDYSYYLSTNGSIKGFVPLIVRVVKQI 194

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
            VNLKRIHS GVKKI +TALGPLQCVP+IT+ S FK CNS+L  LV+ HNLLL QAVDKL
Sbjct: 195 GVNLKRIHSLGVKKISITALGPLQCVPEITSSSDFKHCNSSLALLVDFHNLLLRQAVDKL 254

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T NDSPFFIL+L++AFLSIIQNKGDPQG+I F T ++PCC G SP Y CGSVDE G 
Sbjct: 255 NKET-NDSPFFILDLHAAFLSIIQNKGDPQGSIKFETPLKPCCYGASPGYYCGSVDEDGQ 314

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQH 344
           KKYVLC+ PNS+F+WD VHPTQ+GWIAALT LISTMKQH
Sbjct: 315 KKYVLCEDPNSAFFWDEVHPTQQGWIAALTFLISTMKQH 347

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BLAST of Lag0012747 vs. NCBI nr
Match: KAG7034511.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.0 bits (1403), Expect = 4.5e-151
Identity = 264/339 (77.88%), Postives = 289/339 (85.25%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG G A HHHH      HRH   SKLFVFGDSYVDTGN +PSD NYPK PYG+TFPG 
Sbjct: 15  LLSGLGFARHHHHQ-----HRHLRPSKLFVFGDSYVDTGNTSPSDPNYPKTPYGVTFPGH 74

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           PDGRFSNGRVLTDYVAR++GLKSPIPYR + K G++GAK G+NFAYGGTGVFKTGYDLPT
Sbjct: 75  PDGRFSNGRVLTDYVARIIGLKSPIPYRVVEKEGVEGAKDGVNFAYGGTGVFKTGYDLPT 134

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQHLIANS+FTP DI SSFALVSVSGNDYSYYLSTNGSI+GFIPLI RVV QI
Sbjct: 135 MTTQIDFLQHLIANSTFTPQDINSSFALVSVSGNDYSYYLSTNGSIKGFIPLIVRVVKQI 194

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
            VNLKRIHS GVKKI +TALGPLQCVP+IT+ S FK CNS+L  LV+ HNLLL QAVDKL
Sbjct: 195 GVNLKRIHSLGVKKISITALGPLQCVPEITSSSDFKHCNSSLALLVDFHNLLLRQAVDKL 254

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T NDSPFFIL+L++AFLSIIQNKGDPQG+I F T +QPCC G SP Y CGSVDE G 
Sbjct: 255 NKET-NDSPFFILDLHAAFLSIIQNKGDPQGSIKFETPLQPCCYGASPGYYCGSVDEDGQ 314

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQH 344
           KKYVLC+ PNS+F+WD VHPTQ+GWIAALT LIS MKQH
Sbjct: 315 KKYVLCEDPNSAFFWDEVHPTQQGWIAALTFLISAMKQH 347

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BLAST of Lag0012747 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.3e-97
Identity = 175/339 (51.62%), Postives = 237/339 (69.91%), Query Frame = 0


Query: 1   MCACLLSGQ--GIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYG 60
           +C  LL G+  G+ G + +HH +     F  +KLFVFGDSY DTGN+  + S+  K+PYG
Sbjct: 15  LCTSLLFGEINGVEGSNQNHHLY----PFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYG 74

Query: 61  ITFPGKPDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKT 120
           ITFPGKP GRFS+GRV TD++A+ VG+KSPIPY   +  G +  + G+NFAYGGTGVF T
Sbjct: 75  ITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGTGVFNT 134

Query: 121 GYDLPTMTTQIHFLQHLIANSS-FTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLI 180
              LP MTTQI   Q+++     + P ++ SS ALVSV+GNDYS +++ N     F   I
Sbjct: 135 QTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFI 194

Query: 181 QRVVNQISVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLL 240
           ++VV+Q  VNL+RIH+ GVKKI V +L PL C+P  T ++ F++CN T   LVNLHN LL
Sbjct: 195 KQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLL 254

Query: 241 TQAVDKLNNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCG 300
            Q V KLNN+T   S F IL+LY+AFL++ +NKG   G+  F + ++PCC+G+S EYNCG
Sbjct: 255 QQVVAKLNNET-KQSTFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVSREYNCG 314

Query: 301 SVDEHGNKKYVLCDAPNSSFYWDGVHPTQEGWIAALTTL 337
           SVDE G KKY++CD P ++F+WDG+HPT+EGW +  + L
Sbjct: 315 SVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVL 348

[Close]

BLAST of Lag0012747 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.4e-84
Identity = 152/331 (45.92%), Postives = 219/331 (66.16%), Query Frame = 0


Query: 13  GHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNG 72
           G    +  H   +    ++LFVFGDSY DTGN+  S S+  K PYGITFP KP GRFS+G
Sbjct: 21  GAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDG 80

Query: 73  RVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKT-GYDLPTMTTQIHF 132
           RV TD++AR +G+KSPIPY   +  G +    G+N+AYGGTGVFKT    LP MTTQI +
Sbjct: 81  RVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDY 140

Query: 133 LQH-LIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKR 192
            Q  L A + ++P D+ SS ALVSV+GNDY+ +L+    +      +++VV+QI+VN  R
Sbjct: 141 FQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMR 200

Query: 193 IHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQTNN 252
           IH  GV KI++ ++ PL C+P IT  + F++CN+T     NLHN LL +A+ +LNN+T  
Sbjct: 201 IHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNET-K 260

Query: 253 DSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLC 312
            S F +L+ Y+AFL++ +NKG   G   F   ++PCC+G++  Y+C +VDE G KKY++C
Sbjct: 261 PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIIC 320

Query: 313 DAPNSSFYWDGVHPTQEGWIAALTTLISTMK 342
           + P ++F+WD  HP++EGW +  + L   +K
Sbjct: 321 EDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

[Close]

BLAST of Lag0012747 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 127/314 (40.45%), Postives = 186/314 (59.24%), Query Frame = 0


Query: 28  ELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLKS 87
           ++ KLFVFGDSY DTGN    D+     PYGITFPGKP GR+ +G + TD++ +V+G +S
Sbjct: 11  KVPKLFVFGDSYADTGN-TKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 70

Query: 88  PIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYD--LPTMTTQIHFLQHLIANSSFTPDD 147
           P  YR   +   +G K G+NFA+GG+ +  +  +   P +T Q++FL  L+       D 
Sbjct: 71  PYLYRTHGRD--KGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDI 130

Query: 148 IKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTALG 207
             S  +L+S +G DY YY+  N    G   L+++VV+ + VN+  +     KKI VT+L 
Sbjct: 131 TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQ 190

Query: 208 PLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQT---NNDSPFFILNLYSA 267
           P+ C+P  T+ S FK CN + + LV LHN LL + V KLN Q+     +  FFI+++++A
Sbjct: 191 PIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNA 250

Query: 268 FLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDGV 327
           F+++++NKG  +    F   M+ CC G      CG   + G K Y LCD P S F+WD V
Sbjct: 251 FMTVMKNKGSKR----FKNPMKSCCEGY-----CGRSSD-GGKLYTLCDDPKSFFFWDAV 310

Query: 328 HPTQEGWIAALTTL 337
           HPTQEGW +  + L
Sbjct: 311 HPTQEGWRSIYSVL 311

[Close]

BLAST of Lag0012747 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 238.0 bits (606), Expect = 1.5e-61
Identity = 133/316 (42.09%), Postives = 187/316 (59.18%), Query Frame = 0


Query: 27  FELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLK 86
           F+ +KLFVFGDSY DTGN         ++P GITFPG P GRFS+GRV TDY+A+ +G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 87  SPIPYRHLNKVGLQ--GAKAGINFAYGGTGVFKTGYDL-PTMTTQIHFLQHLIANSSFTP 146
           +PI Y+   K G      K G+NFAYGG G F+T + L PT + QI   + L+  + ++P
Sbjct: 103 TPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 162

Query: 147 DDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTA 206
            D+ SS A  S+ GNDY  Y   NGS +G   L ++VV QI +++KRI   GV+K++V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 207 LGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQ--TNNDSPFFILNLYS 266
             P +C+P +    G   C++  T    LHN LL + + KLN++   NND  F  L+LY+
Sbjct: 223 SPPQKCLPKLVTPKG---CDTNDTS-TYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 267 AFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDG 326
           AF++I +NKG   G  TF    + CC      + CG     G K Y LCD P S F+WD 
Sbjct: 283 AFVTIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDN 342

Query: 327 VHPTQEGWIAALTTLI 338
           VH + +GW +  + L+
Sbjct: 343 VHISDQGWRSVFSLLL 351

[Close]

BLAST of Lag0012747 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 128/306 (41.83%), Postives = 191/306 (62.42%), Query Frame = 0


Query: 31  KLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLKSPIP 90
           KLFVFG+SY DTGN+ P+  ++ K PYGITFPGKP GR+S+G   TD++A+ +G K P  
Sbjct: 34  KLFVFGNSYADTGNMKPTALSW-KLPYGITFPGKPSGRYSDGLTATDFLAKQLGAKLPYL 93

Query: 91  YRHLNKVGLQGAKAGINFAYGGTGVFKTGYD-LPTMTTQIHFLQHL-IANSSFTPD-DIK 150
           +R   K  ++    G+NFA+GG+ VF +  D  P ++TQ+ FL +L +A   +T D D+ 
Sbjct: 94  WRTHGKKKVK-LNRGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLA 153

Query: 151 SSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTALGPL 210
           SS+AL+S SG DY  ++  N ++  +   ++ +V  I  +L  ++    K I VT+L PL
Sbjct: 154 SSYALLSYSGTDYYGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPL 213

Query: 211 QCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQ----TNNDSPFFILNLYSAF 270
            C+P +T  S F+ CN + + LV LHN  L +AV KLN +    T  D  F I++L+ AF
Sbjct: 214 GCLPRVTVASSFRSCNESYSDLVRLHNESLKKAVAKLNKEDKFRTKGDR-FVIVDLHKAF 273

Query: 271 LSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDGVH 330
           ++I++ K    GN  F + ++PCC G     +C  +D  G KKY LC+ P S+F+WD ++
Sbjct: 274 MTILEKK----GNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEIN 327

[Close]

BLAST of Lag0012747 vs. ExPASy TrEMBL
Match: A0A5D3CAN3 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001200 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.6e-156
Identity = 264/340 (77.65%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG+G A HHHHH       HF+ SKLFVFGDSYVDTGNV+PSDSNYPKYPYGIT+PGK
Sbjct: 16  LLSGKGFASHHHHH-----RSHFQPSKLFVFGDSYVDTGNVSPSDSNYPKYPYGITYPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           P GRFS+GRVLTD+ A ++GLKSPIPYRHL KVGL+G K G+NFAYGGTGVFKTG+DLPT
Sbjct: 76  PAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTGVFKTGFDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQ LIANS+FTPD IKSSFALVSVSGNDYSYYLSTNG IQGFIPLI++VV QI
Sbjct: 136 MTTQIDFLQTLIANSTFTPDQIKSSFALVSVSGNDYSYYLSTNGPIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
           SVNL+RIHSFGVKKI +TALGPLQCVP+ITAL+ FK+CNSTL+ LV+ HN LLT+AVD+L
Sbjct: 196 SVNLRRIHSFGVKKISITALGPLQCVPEITALTDFKECNSTLSQLVDFHNFLLTRAVDEL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND PFFILNL+ AF S+IQNKG+PQGNI F   ++PCCIGI+PEYNCGSVD+ GN
Sbjct: 256 NKET-NDYPFFILNLHDAFSSVIQNKGNPQGNIKFENPLKPCCIGINPEYNCGSVDKDGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WDGVHPTQ+GW AALT L+S  KQHF
Sbjct: 316 KKYVLCDDPNSAFFWDGVHPTQQGWFAALTILLSKFKQHF 349

[Close]

BLAST of Lag0012747 vs. ExPASy TrEMBL
Match: A0A1S3CH96 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500885 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.6e-156
Identity = 264/340 (77.65%), Postives = 299/340 (87.94%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG+G A HHHHH       HF+ SKLFVFGDSYVDTGNV+PSDSNYPKYPYGIT+PGK
Sbjct: 16  LLSGKGFASHHHHH-----RSHFQPSKLFVFGDSYVDTGNVSPSDSNYPKYPYGITYPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           P GRFS+GRVLTD+ A ++GLKSPIPYRHL KVGL+G K G+NFAYGGTGVFKTG+DLPT
Sbjct: 76  PAGRFSDGRVLTDFAANLIGLKSPIPYRHLEKVGLKGTKHGVNFAYGGTGVFKTGFDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQ LIANS+FTPD IKSSFALVSVSGNDYSYYLSTNG IQGFIPLI++VV QI
Sbjct: 136 MTTQIDFLQTLIANSTFTPDQIKSSFALVSVSGNDYSYYLSTNGPIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
           SVNL+RIHSFGVKKI +TALGPLQCVP+ITAL+ FK+CNSTL+ LV+ HN LLT+AVD+L
Sbjct: 196 SVNLRRIHSFGVKKISITALGPLQCVPEITALTDFKECNSTLSQLVDFHNFLLTRAVDEL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND PFFILNL+ AF S+IQNKG+PQGNI F   ++PCCIGI+PEYNCGSVD+ GN
Sbjct: 256 NKET-NDYPFFILNLHDAFSSVIQNKGNPQGNIKFENPLKPCCIGINPEYNCGSVDKDGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WDGVHPTQ+GW AALT L+S  KQHF
Sbjct: 316 KKYVLCDDPNSAFFWDGVHPTQQGWFAALTILLSKFKQHF 349

[Close]

BLAST of Lag0012747 vs. ExPASy TrEMBL
Match: A0A0A0KHF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452010 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.9e-153
Identity = 259/340 (76.18%), Postives = 294/340 (86.47%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG+G  GHHHHHHH   H HF+ +KLFVFGDSYVDTGNV+PSDSNYP YPYGIT+PGK
Sbjct: 16  LLSGKGFGGHHHHHHH--VHSHFKPTKLFVFGDSYVDTGNVSPSDSNYPTYPYGITYPGK 75

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           P GRFS+GRVL+D+ A ++G KSPIPYRHL KVG++G K G+NFAYGGTGVFKTGYDLPT
Sbjct: 76  PAGRFSDGRVLSDFAANLIGQKSPIPYRHLEKVGIKGTKYGVNFAYGGTGVFKTGYDLPT 135

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MT+QI FL  LIANS+FTP  I SSFALVSVSGNDYSYYLS NG IQGFIPLI++VV QI
Sbjct: 136 MTSQIDFLHTLIANSTFTPSQINSSFALVSVSGNDYSYYLSQNGPIQGFIPLIEKVVKQI 195

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
           SVNLKRIHSFGVKKI +TALGPL CVP++T L+ FK+CNSTL+ LV+ HN LL QAVD+L
Sbjct: 196 SVNLKRIHSFGVKKIGITALGPLHCVPEVTVLTDFKECNSTLSQLVDFHNHLLKQAVDEL 255

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND PFFILNL+ AFLSIIQNKG PQGNI F T ++PCCIGI+P+YNCGSVD+ GN
Sbjct: 256 NKET-NDLPFFILNLHDAFLSIIQNKGIPQGNIKFETPLKPCCIGINPQYNCGSVDKDGN 315

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQHF 345
           KKYVLCD PNS+F+WDGVHPTQ GWIAALT L+S  KQHF
Sbjct: 316 KKYVLCDDPNSAFFWDGVHPTQRGWIAALTILLSNFKQHF 352

[Close]

BLAST of Lag0012747 vs. ExPASy TrEMBL
Match: A0A6J1EJN1 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111433207 PE=3 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.9e-151
Identity = 263/339 (77.58%), Postives = 289/339 (85.25%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG G A HHHH      HRH + SKLFVFGDSYVDTGN +PSD NYPK PYG+TFPG 
Sbjct: 15  LLSGLGFARHHHHQ-----HRHLQTSKLFVFGDSYVDTGNTSPSDPNYPKTPYGVTFPGH 74

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           PDGRFSNGRVLTDYVAR++GLKSPIPYR + K G++GAK G+NFAYGGTGVFKTGYDLPT
Sbjct: 75  PDGRFSNGRVLTDYVARIIGLKSPIPYRVVEKEGVEGAKDGVNFAYGGTGVFKTGYDLPT 134

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQHLIANS+FTP  I SSFALVSVSGNDYSYYLSTNGSI+GFIPLI RVV QI
Sbjct: 135 MTTQIDFLQHLIANSTFTPQGINSSFALVSVSGNDYSYYLSTNGSIKGFIPLIVRVVKQI 194

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
            VNLKRIHS GVKKI +TALGPLQCVP+IT+ S FK CNS+L  LV+ HNLLL QAVDKL
Sbjct: 195 EVNLKRIHSLGVKKISITALGPLQCVPEITSSSDFKHCNSSLALLVDFHNLLLRQAVDKL 254

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T NDSPFFIL+L++AFLSIIQNKGDPQG+I F T +QPCC G SP Y CGSVDE G 
Sbjct: 255 NKET-NDSPFFILDLHAAFLSIIQNKGDPQGSIKFETPLQPCCYGASPGYYCGSVDEDGQ 314

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQH 344
           KKYVLC+ PNS+F+WD VHPTQ+GWIAALT LIS MKQH
Sbjct: 315 KKYVLCEDPNSAFFWDEVHPTQQGWIAALTFLISAMKQH 347

[Close]

BLAST of Lag0012747 vs. ExPASy TrEMBL
Match: A0A6J1IVU4 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478860 PE=3 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 3.5e-149
Identity = 260/339 (76.70%), Postives = 289/339 (85.25%), Query Frame = 0


Query: 5   LLSGQGIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGK 64
           LLSG G+A HHHH      HRH +LSKLFVFGD YVDTGN +PSD NYPK PYG+TFPG 
Sbjct: 19  LLSGLGLARHHHHQ-----HRHLQLSKLFVFGDLYVDTGNTSPSDPNYPKTPYGVTFPGH 78

Query: 65  PDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYDLPT 124
           PDGRFSNGRVLT Y AR++GLKSPIPYR + K G++GAK G+NFAYGGTGVFKTGYDLPT
Sbjct: 79  PDGRFSNGRVLTSYAARILGLKSPIPYRVVEKEGVEGAKDGVNFAYGGTGVFKTGYDLPT 138

Query: 125 MTTQIHFLQHLIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQI 184
           MTTQI FLQHLIANS+FTP DI SSFALVSVSGNDYSYYLSTNGSI+GFIPLI RVV QI
Sbjct: 139 MTTQIDFLQHLIANSTFTPQDINSSFALVSVSGNDYSYYLSTNGSIKGFIPLIVRVVKQI 198

Query: 185 SVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKL 244
            VNLKRIHS GVKKI +TALGPLQCVP+IT+ S FK CNS+L  LV+ HNLLL QAVDKL
Sbjct: 199 GVNLKRIHSLGVKKISITALGPLQCVPEITSSSDFKHCNSSLALLVDFHNLLLRQAVDKL 258

Query: 245 NNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGN 304
           N +T ND+PFFIL+L++AFLSIIQNKGDPQG+I F+T +QPCC G S  Y CGSVDE G 
Sbjct: 259 NKET-NDAPFFILDLHAAFLSIIQNKGDPQGSIKFATPLQPCCHGTSRGYYCGSVDEDGQ 318

Query: 305 KKYVLCDAPNSSFYWDGVHPTQEGWIAALTTLISTMKQH 344
           KKYVLC+ PNS+F+WD VHPTQ+GWIAALT LIS MKQH
Sbjct: 319 KKYVLCEDPNSAFFWDDVHPTQQGWIAALTFLISAMKQH 351

[Close]

BLAST of Lag0012747 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 357.1 bits (915), Expect = 1.6e-98
Identity = 175/339 (51.62%), Postives = 237/339 (69.91%), Query Frame = 0


Query: 1   MCACLLSGQ--GIAGHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYG 60
           +C  LL G+  G+ G + +HH +     F  +KLFVFGDSY DTGN+  + S+  K+PYG
Sbjct: 15  LCTSLLFGEINGVEGSNQNHHLY----PFRPTKLFVFGDSYADTGNIKKAFSSSWKFPYG 74

Query: 61  ITFPGKPDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKT 120
           ITFPGKP GRFS+GRV TD++A+ VG+KSPIPY   +  G +  + G+NFAYGGTGVF T
Sbjct: 75  ITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGTGVFNT 134

Query: 121 GYDLPTMTTQIHFLQHLIANSS-FTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLI 180
              LP MTTQI   Q+++     + P ++ SS ALVSV+GNDYS +++ N     F   I
Sbjct: 135 QTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFI 194

Query: 181 QRVVNQISVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLL 240
           ++VV+Q  VNL+RIH+ GVKKI V +L PL C+P  T ++ F++CN T   LVNLHN LL
Sbjct: 195 KQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLL 254

Query: 241 TQAVDKLNNQTNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCG 300
            Q V KLNN+T   S F IL+LY+AFL++ +NKG   G+  F + ++PCC+G+S EYNCG
Sbjct: 255 QQVVAKLNNET-KQSTFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVSREYNCG 314

Query: 301 SVDEHGNKKYVLCDAPNSSFYWDGVHPTQEGWIAALTTL 337
           SVDE G KKY++CD P ++F+WDG+HPT+EGW +  + L
Sbjct: 315 SVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVL 348

[Close]

BLAST of Lag0012747 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 312.4 bits (799), Expect = 4.6e-85
Identity = 152/331 (45.92%), Postives = 219/331 (66.16%), Query Frame = 0


Query: 13  GHHHHHHHHRYHRHFELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNG 72
           G    +  H   +    ++LFVFGDSY DTGN+  S S+  K PYGITFP KP GRFS+G
Sbjct: 21  GAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDG 80

Query: 73  RVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGTGVFKT-GYDLPTMTTQIHF 132
           RV TD++AR +G+KSPIPY   +  G +    G+N+AYGGTGVFKT    LP MTTQI +
Sbjct: 81  RVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDNPLPNMTTQIDY 140

Query: 133 LQH-LIANSSFTPDDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKR 192
            Q  L A + ++P D+ SS ALVSV+GNDY+ +L+    +      +++VV+QI+VN  R
Sbjct: 141 FQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQVVDQIAVNAMR 200

Query: 193 IHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQTNN 252
           IH  GV KI++ ++ PL C+P IT  + F++CN+T     NLHN LL +A+ +LNN+T  
Sbjct: 201 IHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHKAIARLNNET-K 260

Query: 253 DSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLC 312
            S F +L+ Y+AFL++ +NKG   G   F   ++PCC+G++  Y+C +VDE G KKY++C
Sbjct: 261 PSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIIC 320

Query: 313 DAPNSSFYWDGVHPTQEGWIAALTTLISTMK 342
           + P ++F+WD  HP++EGW +  + L   +K
Sbjct: 321 EDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

[Close]

BLAST of Lag0012747 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 127/314 (40.45%), Postives = 186/314 (59.24%), Query Frame = 0


Query: 28  ELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLKS 87
           ++ KLFVFGDSY DTGN    D+     PYGITFPGKP GR+ +G + TD++ +V+G +S
Sbjct: 11  KVPKLFVFGDSYADTGN-TKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 70

Query: 88  PIPYRHLNKVGLQGAKAGINFAYGGTGVFKTGYD--LPTMTTQIHFLQHLIANSSFTPDD 147
           P  YR   +   +G K G+NFA+GG+ +  +  +   P +T Q++FL  L+       D 
Sbjct: 71  PYLYRTHGRD--KGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDI 130

Query: 148 IKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTALG 207
             S  +L+S +G DY YY+  N    G   L+++VV+ + VN+  +     KKI VT+L 
Sbjct: 131 TPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQ 190

Query: 208 PLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQT---NNDSPFFILNLYSA 267
           P+ C+P  T+ S FK CN + + LV LHN LL + V KLN Q+     +  FFI+++++A
Sbjct: 191 PIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNA 250

Query: 268 FLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDGV 327
           F+++++NKG  +    F   M+ CC G      CG   + G K Y LCD P S F+WD V
Sbjct: 251 FMTVMKNKGSKR----FKNPMKSCCEGY-----CGRSSD-GGKLYTLCDDPKSFFFWDAV 310

Query: 328 HPTQEGWIAALTTL 337
           HPTQEGW +  + L
Sbjct: 311 HPTQEGWRSIYSVL 311

[Close]

BLAST of Lag0012747 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 133/316 (42.09%), Postives = 187/316 (59.18%), Query Frame = 0


Query: 27  FELSKLFVFGDSYVDTGNVAPSDSNYPKYPYGITFPGKPDGRFSNGRVLTDYVARVVGLK 86
           F+ +KLFVFGDSY DTGN         ++P GITFPG P GRFS+GRV TDY+A+ +G++
Sbjct: 43  FKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVR 102

Query: 87  SPIPYRHLNKVGLQ--GAKAGINFAYGGTGVFKTGYDL-PTMTTQIHFLQHLIANSSFTP 146
           +PI Y+   K G      K G+NFAYGG G F+T + L PT + QI   + L+  + ++P
Sbjct: 103 TPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVYSP 162

Query: 147 DDIKSSFALVSVSGNDYSYYLSTNGSIQGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTA 206
            D+ SS A  S+ GNDY  Y   NGS +G   L ++VV QI +++KRI   GV+K++V  
Sbjct: 163 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 222

Query: 207 LGPLQCVPDITALSGFKQCNSTLTPLVNLHNLLLTQAVDKLNNQ--TNNDSPFFILNLYS 266
             P +C+P +    G   C++  T    LHN LL + + KLN++   NND  F  L+LY+
Sbjct: 223 SPPQKCLPKLVTPKG---CDTNDTS-TYLHNSLLRKGLIKLNDKEINNNDKSFMTLDLYN 282

Query: 267 AFLSIIQNKGDPQGNITFSTAMQPCCIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDG 326
           AF++I +NKG   G  TF    + CC      + CG     G K Y LCD P S F+WD 
Sbjct: 283 AFVTIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFWDN 342

Query: 327 VHPTQEGWIAALTTLI 338
           VH + +GW +  + L+
Sbjct: 343 VHISDQGWRSVFSLLL 351

[Close]

BLAST of Lag0012747 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-31
Identity = 91/283 (32.16%), Postives = 135/283 (47.70%), Query Frame = 0


Query: 54  KYPYGITFPGKPDGRFSNGRVLTDYVARVVGLKSPIPYRHLNKVGLQGAKAGINFAYGGT 113
           K PYGITFPGKP GR+S+G   TD++                                G+
Sbjct: 9   KLPYGITFPGKPSGRYSDGLTATDFL--------------------------------GS 68

Query: 114 GVFKTGYD-LPTMTTQIHFLQHL-IANSSFTPD-DIKSSFALVSVSGNDYSYYLSTNGSI 173
            VF +  D  P ++TQ+ FL +L +A   +T D D+ SS+AL+S SG DY  ++  N ++
Sbjct: 69  EVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNM 128

Query: 174 QGFIPLIQRVVNQISVNLKRIHSFGVKKIIVTALGPLQCVPDITALSGFKQCNSTLTPLV 233
             +   ++ +V  I                                     CN + + LV
Sbjct: 129 AAYPAFVEFIVEDI------------------------------------HCNESYSDLV 188

Query: 234 NLHNLLLTQAVDKLNNQ----TNNDSPFFILNLYSAFLSIIQNKGDPQGNITFSTAMQPC 293
            LHN  L +AV KLN +    T  D  F I++L+ AF++I++ K    GN  F + ++PC
Sbjct: 189 RLHNESLKKAVAKLNKEDKFRTKGDR-FVIVDLHKAFMTILEKK----GNKRFKSPLKPC 213

Query: 294 CIGISPEYNCGSVDEHGNKKYVLCDAPNSSFYWDGVHPTQEGW 330
           C G     +C  +D  G KKY LC+ P S+F+WD ++PTQEGW
Sbjct: 249 CEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGW 213

The following BLAST results are available for this feature:

BLAST of Lag0012747 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038883064.1	2.8e-161	80.29	GDSL esterase/lipase At5g03610-like [Benincasa hispida]	[more]
XP_008462554.1	1.4e-155	77.65	PREDICTED: GDSL esterase/lipase At5g03610-like [Cucumis melo] >KAA0025272.1 GDSL...	[more]
XP_004143312.1	1.8e-152	76.18	GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48249.1 hypothetical protei...	[more]
XP_023544735.1	4.5e-151	77.29	GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo]	[more]
KAG7034511.1	4.5e-151	77.88	GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lag0012747 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZS7	2.3e-97	51.62	GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2...	[more]
Q9SF94	6.4e-84	45.92	GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2...	[more]
Q9LZS8	1.8e-62	40.45	GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3...	[more]
Q8RWJ4	1.5e-61	42.09	GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2...	[more]
Q9LZS9	1.7e-60	41.83	GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3...	[more]

BLAST of Lag0012747 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CAN3	6.6e-156	77.65	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202...	[more]
A0A1S3CH96	6.6e-156	77.65	GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500885 PE=3...	[more]
A0A0A0KHF9	8.9e-153	76.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452010 PE=3 SV=1	[more]
A0A6J1EJN1	4.9e-151	77.58	GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC11143320...	[more]
A0A6J1IVU4	3.5e-149	76.70	GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478860 ...	[more]

BLAST of Lag0012747 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03610.1	1.6e-98	51.62	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT3G09930.1	4.6e-85	45.92	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G03600.1	1.3e-63	40.45	SGNH hydrolase-type esterase superfamily protein	[more]
AT2G36325.1	1.1e-62	42.09	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G03590.1	1.3e-31	32.16	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 15..343 e-value: 2.6E-55 score: 190.0
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 32..328 e-value: 6.1E-27 score: 95.5
None	No IPR available	PANTHER	PTHR46020:SF4	OSJNBB0059K02.9 PROTEIN	coord: 14..342
None	No IPR available	PANTHER	PTHR46020	OSJNBB0059K02.9 PROTEIN	coord: 14..342
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 31..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0012747.1	Lag0012747.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds