Lag0012626 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012626
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like superfamily protein
Locationchr1: 42774838 .. 42775755 (+)
RNA-Seq ExpressionLag0012626
SyntenyLag0012626
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCATCTTGGTGCTTCTTATTCTATTATGCTTAGTTTTATTTTTCTTTGTACTCCTCCAGAAAAAAAAAAAAAATTAAAGTGATTTTTCCAAACCCTTTAGGTTTCTTTTCTATTTTGTTTTTTAATCATAGCCTCTGCCTCTTCCTCAGAGGTAGAATCGAAATGGTGGGCGAAGGTTTGGAAATTAAAAGTTCCAAATAAGGTAAAGCACTTTGTTTGGAAATCTTTTCATGAAACTATTCCCACTATAACTAACCTCTGGAGGCACCATGTTCCTGTTAGTGGATGCTGCCCAGTTTGTCGGGAGAAGACGGAGACCACTGATCATGCTCTGTTTTTGTGTTCTCGAGCTCGAGAGGTGTGGGAGGGTATTCTCCCATTGATGAACAAAGGGTTTTGGAAACCAATGGATATCAAAGACAGATGGTTGAGTCTTGGAGACTGTCAAAGCAAGCAGTTAGATCTTATTAGTATTGGGGCTTGGGCGATTTGGAATGATAAGAACAATGTTTTTCACCAAAGGCCAGTTCCTAATGTCCAGACTCGAAGTGAGTGGATTCTAGAGTATTTGGACGAATTCCAAATAGCTAATATAGTGGGTGGAGCTGTTAATCAAACAGTGGATGATGTTCGAAGAATTCTACAAGACGCTGAAGAGATAATTATGCGTTGTGATGCGGCTTACGATGAGATTAATTGTACGGTGGGTATTGGACTGGTGTTCCGGGAGAGACAAGGGAATCTTAAGGCAGTGATCAAGGTTATGTCTAAAATTAGCAGTACATCACCGCTGGGAGCGGAAGTGGGAGCAGTCCTCCAAAGACTTCGATTTGCTCGATCTCTAAAGATGCAATGCTTATCGGTGGTGTCCGACTCCTTATCGTTTATAAGGACAGTCAGGGGAGAAGTGTAG

mRNA sequence

ATGCCTCATCTTGCCTCTGCCTCTTCCTCAGAGGTAGAATCGAAATGGTGGGCGAAGGTTTGGAAATTAAAAGTTCCAAATAAGGTAAAGCACTTTGTTTGGAAATCTTTTCATGAAACTATTCCCACTATAACTAACCTCTGGAGGCACCATGTTCCTGTTAGTGGATGCTGCCCAGTTTGTCGGGAGAAGACGGAGACCACTGATCATGCTCTGTTTTTGTGTTCTCGAGCTCGAGAGGTGTGGGAGGGTATTCTCCCATTGATGAACAAAGGGTTTTGGAAACCAATGGATATCAAAGACAGATGGTTGAGTCTTGGAGACTGTCAAAGCAAGCAGTTAGATCTTATTAGTATTGGGGCTTGGGCGATTTGGAATGATAAGAACAATGTTTTTCACCAAAGGCCAGTTCCTAATGTCCAGACTCGAAGTGAGTGGATTCTAGAGTATTTGGACGAATTCCAAATAGCTAATATAGTGGGTGGAGCTGTTAATCAAACAGTGGATGATGTTCGAAGAATTCTACAAGACGCTGAAGAGATAATTATGCGTTGTGATGCGGCTTACGATGAGATTAATTGTACGGTGGGTATTGGACTGGTGTTCCGGGAGAGACAAGGGAATCTTAAGGCAGTGATCAAGGTTATGTCTAAAATTAGCAGTACATCACCGCTGGGAGCGGAAGTGGGAGCAGTCCTCCAAAGACTTCGATTTGCTCGATCTCTAAAGATGCAATGCTTATCGGTGGTGTCCGACTCCTTATCGTTTATAAGGACAGTCAGGGGAGAAGTGTAG

Coding sequence (CDS)

ATGCCTCATCTTGCCTCTGCCTCTTCCTCAGAGGTAGAATCGAAATGGTGGGCGAAGGTTTGGAAATTAAAAGTTCCAAATAAGGTAAAGCACTTTGTTTGGAAATCTTTTCATGAAACTATTCCCACTATAACTAACCTCTGGAGGCACCATGTTCCTGTTAGTGGATGCTGCCCAGTTTGTCGGGAGAAGACGGAGACCACTGATCATGCTCTGTTTTTGTGTTCTCGAGCTCGAGAGGTGTGGGAGGGTATTCTCCCATTGATGAACAAAGGGTTTTGGAAACCAATGGATATCAAAGACAGATGGTTGAGTCTTGGAGACTGTCAAAGCAAGCAGTTAGATCTTATTAGTATTGGGGCTTGGGCGATTTGGAATGATAAGAACAATGTTTTTCACCAAAGGCCAGTTCCTAATGTCCAGACTCGAAGTGAGTGGATTCTAGAGTATTTGGACGAATTCCAAATAGCTAATATAGTGGGTGGAGCTGTTAATCAAACAGTGGATGATGTTCGAAGAATTCTACAAGACGCTGAAGAGATAATTATGCGTTGTGATGCGGCTTACGATGAGATTAATTGTACGGTGGGTATTGGACTGGTGTTCCGGGAGAGACAAGGGAATCTTAAGGCAGTGATCAAGGTTATGTCTAAAATTAGCAGTACATCACCGCTGGGAGCGGAAGTGGGAGCAGTCCTCCAAAGACTTCGATTTGCTCGATCTCTAAAGATGCAATGCTTATCGGTGGTGTCCGACTCCTTATCGTTTATAAGGACAGTCAGGGGAGAAGTGTAG

Protein sequence

MPHLASASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRWLSLGDCQSKQLDLISIGAWAIWNDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRRILQDAEEIIMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLRFARSLKMQCLSVVSDSLSFIRTVRGEV
Homology
BLAST of Lag0012626 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 140.2 bits (352), Expect = 2.6e-29
Identity = 81/267 (30.34%), Postives = 133/267 (49.81%), Query Frame = 0

Query: 5    ASASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREK 64
            A+++S+      W  +WKL VP K+K F+W+S HE IPT  NL    +     C +C ++
Sbjct: 1120 ATSASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDR 1179

Query: 65   TETTDHALFLCSRAREVWEGILPLMN-KGFWKPMDIKDRWLSLGD-CQSKQLDLISIGAW 124
             E+  HA F C RAR++W  + P +        +   + W SL +  + K L+L +I  W
Sbjct: 1180 RESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGW 1239

Query: 125  AIWNDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRRILQ-----D 184
             IWND+N++ H + V  V+ + EW+  +LD    A  +     +T  + R ++Q      
Sbjct: 1240 GIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQ-MSNYSPRTQSNHRPVVQYWRPSS 1299

Query: 185  AEEIIMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLR 244
            +  + +  DAA      +   G + R+   +L A   +       SPL AE+  +L+ L+
Sbjct: 1300 SVSLKLNTDAACR--GASTSFGCIIRDSSCSLVAATSIRVPF-PLSPLLAEIRCILEGLK 1359

Query: 245  FARSLKMQCLSVVSDSLSFIRTVRGEV 265
            FA +     L V SDSL  I+ +R E+
Sbjct: 1360 FAAASNFTHLEVESDSLLAIQLIRNEI 1382

BLAST of Lag0012626 vs. NCBI nr
Match: XP_030498122.1 (uncharacterized protein LOC115713779 [Cannabis sativa])

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-25
Identity = 77/272 (28.31%), Postives = 130/272 (47.79%), Query Frame = 0

Query: 9    SSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETT 68
            SS     WW+K WKL++P+K++ FVWK FH  +P  + L R H+  S  CP+C+ + ET 
Sbjct: 1044 SSNTYEHWWSKFWKLQLPSKLRIFVWKVFHNVLPVASELNRRHIAASPYCPLCKLQQETI 1103

Query: 69   DHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRWLSL---GDCQSKQLDLISIGAWAIW 128
             HALFLCSRA+EVW+  +  +N  F            L   G   + + +      W+IW
Sbjct: 1104 PHALFLCSRAKEVWK--ISNLNLNFKLAASSSAEEFLLYVSGHTSTLEFEQFLTLCWSIW 1163

Query: 129  NDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANI----------------VGGAVNQTVD 188
             ++N  FH +P        ++ L+Y+ ++Q  +                    +   +VD
Sbjct: 1164 FERNAEFHDKPAKCPTAIKDFALQYIAKYQSVHAKSNVPCPDVAPAAPFPTSTSAAPSVD 1223

Query: 189  DVRRILQDAEEIIMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEV 248
              +   Q   +  +  DAA+D+ N  +GIG V R+  G +KA     S   S      E 
Sbjct: 1224 PWKAPPQGTYK--LNTDAAFDKTNKIIGIGAVLRDSSGFIKAAFS-KSIHGSYKAEEMEA 1283

Query: 249  GAVLQRLRFARSLKMQCLSVVSDSLSFIRTVR 262
             A++  L++ +S+ +    + +DSL  ++ ++
Sbjct: 1284 TALVYALQWLQSIGLSTDYIETDSLLVVQGLK 1310

BLAST of Lag0012626 vs. NCBI nr
Match: RYR44318.1 (hypothetical protein Ahy_A08g040672 [Arachis hypogaea])

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-24
Identity = 74/272 (27.21%), Postives = 131/272 (48.16%), Query Frame = 0

Query: 6    SASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKT 65
            S S+S+     W  +WKLKVP K++ F+W++ H  +P   NL++  +  +  CP+C ++ 
Sbjct: 793  SPSTSDDFKDLWRDIWKLKVPQKIRTFLWRASHNILPVFQNLYKKKISNTPICPICLQEP 852

Query: 66   ETTDHALFLCSRAREVWEG--------ILPLMNKGFWKPMDIKDRWLSLGDCQSKQLDLI 125
            E+T+HAL LC   R VW G           +++ G W     K   +  G         +
Sbjct: 853  ESTEHALLLCPWTRAVWFGAQIQCCLTAYTVLSFGKWVMDLFKSMKIGTGTDYVLSSSRV 912

Query: 126  SIGAWAIWNDKNNVFHQRPVPN----VQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRR 185
                W IW  +N   H R  P+    ++   +  +++ D  +   I   +++    D R 
Sbjct: 913  GFLVWEIWKARNQAVHHRSKPSPLLAIENAKQMEIDFADVIEEPAI--SSIHHRRTDRRV 972

Query: 186  ILQDAEEIIMRC--DAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGA 245
              +   +  ++C  DAA+ E+        VFR+  G+L A     S+I+++SPL AE  A
Sbjct: 973  TWRPPPQGWIKCNVDAAFREVYSGGATAAVFRDHTGSLLAASN--SRIAASSPLAAEAFA 1032

Query: 246  VLQRLRFARSLKMQCLSVVSDSLSFIRTVRGE 264
            V + L  A++ ++  + V SDSL  I+ ++ +
Sbjct: 1033 VREALIMAKNFQLDRIIVESDSLLLIQALKSK 1060

BLAST of Lag0012626 vs. NCBI nr
Match: XP_030505432.1 (uncharacterized protein LOC115720422 [Cannabis sativa])

HSP 1 Score: 121.7 bits (304), Expect = 9.4e-24
Identity = 77/256 (30.08%), Postives = 129/256 (50.39%), Query Frame = 0

Query: 6    SASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKT 65
            S+S++   ++WW   W  K+P K+K+F W++FH  +PT  NL+R  V  S  C  C    
Sbjct: 1550 SSSNTSFYNRWWKFFWNQKLPPKIKNFGWRTFHHILPTAFNLYRKKVVPSPSCSFCGGAL 1609

Query: 66   ETTDHALFLCSRAREVWEGILPLMNKGFW---KPMDIKDRWLS-LGDCQSKQLDLISIGA 125
            ET  HAL  CSR R+VW+ + PL +  F+   +  DIKD  LS   D  +    L+    
Sbjct: 1610 ETVTHALLDCSRVRQVWK-LSPLYH--FYISHRNSDIKDFMLSAYTDLNADDFSLLMCTV 1669

Query: 126  WAIWNDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDD-----VRRILQ 185
            W+IW  +N    +   P+  +  +WI  YL +++ A +    V  T  +     V R+ +
Sbjct: 1670 WSIWEFRNKKLFRNANPDAGSLVQWIATYLSQYREAQVGHTRVAPTAQNCNQASVPRVRE 1729

Query: 186  DAEEIIMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRL 245
             + ++    DAA ++ N  VG+G V ++  G + A +  M   +   PL AE  A+   L
Sbjct: 1730 GSYQLC--TDAAINDRNGKVGLGAVIKDWNGQVLAGVS-MPFSAKFDPLMAEAMALRLGL 1789

Query: 246  RFARSLKMQCLSVVSD 253
             +  ++++   S+ +D
Sbjct: 1790 NWCCNVRLPLSSICTD 1799

BLAST of Lag0012626 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 120.2 bits (300), Expect = 2.7e-23
Identity = 68/207 (32.85%), Postives = 109/207 (52.66%), Query Frame = 0

Query: 32  FVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCSRAREVWEGILPLMNK 91
           F+W+ F++ +P++ NL +  +  +  C VC ++ ETTDHALF C RA+EVW  +LP    
Sbjct: 2   FLWRVFNDCVPSMRNLIKRGIDPNPNCLVCGKQIETTDHALFKCKRAKEVWNILLPQAFP 61

Query: 92  GFWKPMDIKDRWLSLGDCQS-KQLDLISIGAWAIWNDKNNVFHQRPVPNVQTRSEWILEY 151
                  ++D  L L +  S    DL+ +G WAIWND+N +  QR +P+ + RS+WIL Y
Sbjct: 62  QCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQRQIPDAKIRSDWILTY 121

Query: 152 LDEFQIANIVG---GAVNQTVDDVRRILQD------AEEIIMRCDAAYDEINCTVGIGLV 211
           + +FQ+ ++       + +      R +++      A  I +  DAA  +     GIG+V
Sbjct: 122 VRDFQVRDVPSLGDFRIQEDASSNDRTIENYWSPPPAGWIKINVDAACKKHQFRTGIGIV 181

Query: 212 FRERQGNLKAVIKVMSKISSTSPLGAE 229
            R  +G + A        +S  PL AE
Sbjct: 182 CRNEKGQILAA-------ASHDPLMAE 201

BLAST of Lag0012626 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 8.3e-07
Identity = 36/138 (26.09%), Postives = 63/138 (45.65%), Query Frame = 0

Query: 20  VWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCSRAR 79
           +WK++VP +VK F+W   ++ + T     R H+  S  C VC+   E+  H L  C    
Sbjct: 291 LWKVRVPERVKTFLWLVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQL 350

Query: 80  EVWEGILP-LMNKGFWKPMDIKDRWLSLGD---CQSKQLDLI-SIGAWAIWNDK-NNVFH 139
            +W  ++P    +GF+     +  + +LGD   C+      I ++  W  W  +  N+F 
Sbjct: 351 GIWVRVVPQRRQQGFFSKSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFG 410

Query: 140 QRPV--PNVQTRSEWILE 150
           +       V+   EW +E
Sbjct: 411 ENTKCRDRVKFVKEWAVE 428

BLAST of Lag0012626 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.2e-29
Identity = 81/267 (30.34%), Postives = 133/267 (49.81%), Query Frame = 0

Query: 5    ASASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREK 64
            A+++S+      W  +WKL VP K+K F+W+S HE IPT  NL    +     C +C ++
Sbjct: 1120 ATSASTNYRGTQWNSIWKLTVPTKIKIFIWRSAHEHIPTAQNLLLRGIGELPACTICGDR 1179

Query: 65   TETTDHALFLCSRAREVWEGILPLMN-KGFWKPMDIKDRWLSLGD-CQSKQLDLISIGAW 124
             E+  HA F C RAR++W  + P +        +   + W SL +  + K L+L +I  W
Sbjct: 1180 RESIIHAFFHCKRARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGW 1239

Query: 125  AIWNDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRRILQ-----D 184
             IWND+N++ H + V  V+ + EW+  +LD    A  +     +T  + R ++Q      
Sbjct: 1240 GIWNDRNSLIHGKQVSPVEFKCEWLTPFLDSHSQAQ-MSNYSPRTQSNHRPVVQYWRPSS 1299

Query: 185  AEEIIMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLR 244
            +  + +  DAA      +   G + R+   +L A   +       SPL AE+  +L+ L+
Sbjct: 1300 SVSLKLNTDAACR--GASTSFGCIIRDSSCSLVAATSIRVPF-PLSPLLAEIRCILEGLK 1359

Query: 245  FARSLKMQCLSVVSDSLSFIRTVRGEV 265
            FA +     L V SDSL  I+ +R E+
Sbjct: 1360 FAAASNFTHLEVESDSLLAIQLIRNEI 1382

BLAST of Lag0012626 vs. ExPASy TrEMBL
Match: A0A803NM27 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 2.4e-25
Identity = 76/260 (29.23%), Postives = 117/260 (45.00%), Query Frame = 0

Query: 8    SSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTET 67
            S S     WW   W+LK+P KVK F WK+ H  +P  + L++     S  C +C    E+
Sbjct: 1450 SVSSPNVSWWKSFWQLKLPPKVKLFAWKAIHNALPVASELYKRKSLTSASCSLCLNAWES 1509

Query: 68   TDHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRWLSLGDCQSK-QLDLISIGAWAIWN 127
              HA+F C  AR VW+      N      M I+D    + +C +K +L++I    W+IW+
Sbjct: 1510 VGHAMFACKHARHVWKIAGFSFNNKAAVSMKIEDFLFQISECYTKSELEMIFCTMWSIWS 1569

Query: 128  DKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIV----GGAVNQTVDDVRRILQDAEEII 187
            D+NNV H +        S     +L  FQ A  +    G A   T    R        ++
Sbjct: 1570 DRNNVLHGKIPQQPSVISAKAASFLSSFQSAQQLSLHAGLAPADTPTAHRAWTPPPPNLL 1629

Query: 188  -MRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLRFARS 247
             +  DAA+D+    +G G + R+  GN+KA +          P   E   +   L++AR 
Sbjct: 1630 KLNVDAAFDDTRKRIGFGAIIRDSTGNVKAAMSHPID-GCCRPQDMEAKGLFYSLKWARQ 1689

Query: 248  LKMQCLSVVSDSLSFIRTVR 262
            L  +   V +DSL  +  +R
Sbjct: 1690 LNFKVDLVETDSLILVNALR 1708

BLAST of Lag0012626 vs. ExPASy TrEMBL
Match: A0A803QAH8 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 4.1e-25
Identity = 72/260 (27.69%), Postives = 115/260 (44.23%), Query Frame = 0

Query: 8    SSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTET 67
            S S     WW   W+LK+P KVK F WK+ H  +P  + L++     S  C +C    E+
Sbjct: 1437 SVSSPHVSWWKSFWQLKLPPKVKLFAWKAIHNALPVASELYKRKSLTSASCSLCLNAWES 1496

Query: 68   TDHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRWLSLGDCQSK-QLDLISIGAWAIWN 127
              HA+F C  AR VW+ +    N      M I+D    + +C +K +L++I    W+IW+
Sbjct: 1497 VGHAMFTCKHARHVWKIVGFSFNNKAVVSMKIEDFLFQISECYTKSELEMIFCTMWSIWS 1556

Query: 128  DKNNVFHQRPVPNVQTRSEWILEYLDEFQIA---NIVGGAVNQTVDDVRRILQDAEEIIM 187
            D+NNV H +        S     +L  FQ A   ++  G          +         +
Sbjct: 1557 DRNNVIHGKLAQQPSVISAKAASFLSSFQSAHQLSLHAGLAPADTPTAHKTWTPPPPTFL 1616

Query: 188  R--CDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLRFARS 247
            +   DAA+D+    +G G + R+  G +KA +          P   E   +   L++AR 
Sbjct: 1617 KLNVDAAFDDTRKRIGFGAIIRDSTGKVKAAMSHPID-GCCRPQDMEAKGLFYSLKWARQ 1676

Query: 248  LKMQCLSVVSDSLSFIRTVR 262
            L  +   V +DSL  +  +R
Sbjct: 1677 LNFKVDMVETDSLILVNALR 1695

BLAST of Lag0012626 vs. ExPASy TrEMBL
Match: A0A803PCG2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 4.1e-25
Identity = 76/261 (29.12%), Postives = 121/261 (46.36%), Query Frame = 0

Query: 6    SASSSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKT 65
            S S+      WW  +W LK+P K+KHF WK+F+  +P   NL+  H   S  C  C  + 
Sbjct: 877  STSNPNPYKDWWKTLWTLKIPPKIKHFTWKAFNHLLPCSLNLFLKHSIPSPACSFCGAEA 936

Query: 66   ETTDHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRW-LSLGDCQSKQLDLISIGAWAI 125
            E+  HAL  C++ +++W   +          +DIKD + LSL      Q+ L     W I
Sbjct: 937  ESVSHALLYCTKVKQIWHYTIFKQFFQDHSRLDIKDFYLLSLCQLPKDQIPLFLGIIWQI 996

Query: 126  WNDKNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRRILQDA---EEI 185
            WN +N    Q+  P    + E ++ +L E+Q A  +    + +V     +L         
Sbjct: 997  WNIRNACLFQKHSPQANVQ-ESVVSFLQEYQEAQAIPSTSHHSVTHCSAVLDQVLTPTTT 1056

Query: 186  IMRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVGAVLQRLRFARS 245
             +  DAA +  N   G GLVF +    +    K+  +  ++SPL AE  A+ + L++  +
Sbjct: 1057 ALFVDAALNTHNPATGFGLVFMQGLSQVHQSTKIF-RPGASSPLFAEAQALYEGLKWCIA 1116

Query: 246  LKMQCLSVVSDSLSFIRTVRG 263
              +Q   V SD L+ I  V G
Sbjct: 1117 SNLQPRFVFSDCLNLITKVNG 1135

BLAST of Lag0012626 vs. ExPASy TrEMBL
Match: A0A803PH33 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 2.7e-24
Identity = 77/271 (28.41%), Postives = 126/271 (46.49%), Query Frame = 0

Query: 9   SSEVESKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETT 68
           SS     WW+K WKL++P+K++ FVWK FH  +P  + L R H+  S  CP+C+ + ET 
Sbjct: 251 SSNTYEHWWSKFWKLQLPSKLRIFVWKVFHNVLPVASELNRRHIAASPYCPLCKLQQETI 310

Query: 69  DHALFLCSRAREVWEGILPLMNKGFWKPMDIKDRWLSLGDCQSK-QLDLISIGAWAIWND 128
            HALFLCSRA+EVW      +N         ++  L + +  S  + +      W+IW +
Sbjct: 311 PHALFLCSRAKEVWRISGLNLNFKLAASSSAEEFLLYVSEHTSTLEFEQFLTLCWSIWFE 370

Query: 129 KNNVFHQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVRRILQDAEEII----- 188
           +N  FH +P        ++ L+Y+ ++Q    V    N    DV                
Sbjct: 371 RNAEFHDKPAKCPTAIKDFALQYITKYQ---SVHDKPNVPSPDVAPAAPFPTSTFAAPSV 430

Query: 189 ------------MRCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKISSTSPLGAEVG 248
                       +  DAA+D+ N  +GIG V R+  G ++A     S   S      E  
Sbjct: 431 DPWKAPPPGTYKLNTDAAFDKTNKIIGIGAVLRDSSGFIQAAFS-KSIHGSYKVEEMEAT 490

Query: 249 AVLQRLRFARSLKMQCLSVVSDSLSFIRTVR 262
           A++  L++ RS+ +    + +DSL  ++ ++
Sbjct: 491 ALVYALQWLRSIGLSTDYIETDSLLVVQGLK 517

BLAST of Lag0012626 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-13
Identity = 45/160 (28.12%), Postives = 72/160 (45.00%), Query Frame = 0

Query: 18  AKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCSR 77
           AK+WKLK   K+KHF+WK     + T  NL R H+     C  C ++ ET+ H  F C  
Sbjct: 16  AKIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFY 75

Query: 78  AREVW--EGILPLMNKGFWKPMDIKDRWLSLGDCQSKQLDLISIG---AWAIWNDKNNVF 137
           A++VW   GI     +     M+ K   L      ++Q  L ++     W +W  +N + 
Sbjct: 76  AQQVWRASGIPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQLV 135

Query: 138 HQRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTVDDVR 173
            Q+   + Q   +     + E++  N    ++NQ V   R
Sbjct: 136 FQQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSSR 175

BLAST of Lag0012626 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.0e-12
Identity = 61/235 (25.96%), Postives = 98/235 (41.70%), Query Frame = 0

Query: 17  WAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCS 76
           + K+WK +   K++HF+WK    ++P    L   H+     C  C    ET +H LF C+
Sbjct: 254 YQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCT 313

Query: 77  RAREVWE-GILPLMNKGFW-KPMDIKDRW---LSLGDCQ-SKQLDLISIGAWAIWNDKNN 136
            AR  W    +P+   G W   + +   W   L  G+ Q  K   L+    W +W ++N 
Sbjct: 314 FARLTWAISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKASQLVPWLLWRLWKNRNE 373

Query: 137 VFHQRPVPNVQTRSEWILEYLDEFQI---ANIVGGAVNQTVDDVRRILQDAEE-IIMRCD 196
           +  +    N Q       + L+E++I   A   G           R      + +    D
Sbjct: 374 LVFRGREFNAQEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGRWRPPPHQWVKCNTD 433

Query: 197 AAYDEINCTVGIGLVFRERQGNLKAV-IKVMSKISST--SPLGAEVGAVLQRLRF 239
           A ++  N   GIG V R  +G +K +  + + K+ S   + L A   AVL   RF
Sbjct: 434 ATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRF 488

BLAST of Lag0012626 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-09
Identity = 59/258 (22.87%), Postives = 105/258 (40.70%), Query Frame = 0

Query: 20  VWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALFLCSRAR 79
           +WKL V  K+KHF+W+     + T T L   ++     C  C  + ET  H +F C   +
Sbjct: 37  IWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQ 96

Query: 80  EVWEGILPLMNKGFWKPMDIKD---RWLSLGDCQ-SKQLD--LISIGAWAIWNDKNNVFH 139
            VW     ++   +  P   +D   R + L   Q +  LD  L     W +W  +N    
Sbjct: 97  SVWRSANIIIGNQWGPPSSFEDNLNRLIQLSKTQTTNSLDRFLPFWIMWRLWKSRNVFLF 156

Query: 140 QRPVPNVQTRSEWILEYLDEFQIANIVGGAVNQTV--DDVRRILQDAEE--------IIM 199
           Q+   +    +   ++   E+  AN      N  V  + ++   +D+ +        +  
Sbjct: 157 QQKCQSPDYEARKGIQDATEWLNANETTENTNVHVATNPIQTSRRDSSQWNPPPEGWVKC 216

Query: 200 RCDAAYDEINCTVGIGLVFRERQGNLKAVIKVMSKI-SSTSPLGAEVGAVLQRLRFARSL 259
             D+ Y + +     G   RE  G++  V+   +K+ SST  L AE    L  L+   + 
Sbjct: 217 NFDSGYTQGSPYTRSGWTIRECNGHI--VLCGNAKLQSSTCSLHAEALGFLHALQVIWAH 276

Query: 260 KMQCLSVVSDSLSFIRTV 261
            ++ +   SDS S +  +
Sbjct: 277 GLRYVWFESDSKSLVTLI 292

BLAST of Lag0012626 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.8e-05
Identity = 23/69 (33.33%), Postives = 35/69 (50.72%), Query Frame = 0

Query: 14 SKWWAKVWKLKVPNKVKHFVWKSFHETIPTITNLWRHHVPVSGCCPVCREKTETTDHALF 73
          + W   +W LK+  K+K  +WK+ +  +P    L   ++ +   C  CR+  ET  H LF
Sbjct: 3  NNWIGDIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCRD-FETITHILF 62

Query: 74 LCSRA-REV 82
           C  A REV
Sbjct: 63 NCPFAQREV 70

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.12.6e-2930.34uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_030498122.12.2e-2528.31uncharacterized protein LOC115713779 [Cannabis sativa][more]
RYR44318.11.9e-2427.21hypothetical protein Ahy_A08g040672 [Arachis hypogaea][more]
XP_030505432.19.4e-2430.08uncharacterized protein LOC115720422 [Cannabis sativa][more]
XP_022145060.12.7e-2332.85uncharacterized protein LOC111014578 [Momordica charantia][more]
Match NameE-valueIdentityDescription
P0C2F68.3e-0726.09Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A6J1DX301.2e-2930.34uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A803NM272.4e-2529.23Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QAH84.1e-2527.69Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PCG24.1e-2529.12Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PH332.7e-2428.41Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G25270.11.6e-1328.13Ribonuclease H-like superfamily protein [more]
AT4G29090.14.0e-1225.96Ribonuclease H-like superfamily protein [more]
AT2G02650.11.8e-0922.87Ribonuclease H-like superfamily protein [more]
AT3G26855.12.8e-0533.33RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 10..82
e-value: 7.0E-18
score: 65.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 186..263
e-value: 1.1E-5
score: 25.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 179..264
e-value: 5.1E-5
score: 25.3
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 9..242
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 9..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012626.1Lag0012626.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity