Lag0012599 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012599
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionMitochondrial import inner membrane translocase subunit TIM14
Locationchr1: 42561647 .. 42562798 (-)
RNA-Seq ExpressionLag0012599
SyntenyLag0012599
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCAGTCTTCGCTCATTCTTCGTTTCTAGTATGTCGTGTCCATGCGGCGGGTGGAATTTTCTCATCATTTCTTTGTTTGATTTTGTGTTGGTTGCTGTTATATTTTCGTTCGGGAACTTGAAATTGTTGATTCTTTTTTGGGTTTTGATTATTTTGGAATTTTAAAGACTGCGGTATTGCATTTTGTATCTGTGGTTGTCGGGGTTTGTTTATTTCGTGAGAAAATTGGAAGTATTTTGGATCATAAACAAATCTTAGCTCGTCAAGAAAATTTGTGAACTTTCGCTGTACTCTGCTTGATAGACAATCGATCCGAGTTCCCTAGCTAGCAAATTTTGAGTTCAGTTGTTTTCATTTTGATTTTATGGGATTTTCTCCCTTACTAGCGCCTCTGCTGCTTTAATCTTGTGGAGTGGTTTGCAGCATTCCTATTCCTTCTTTTATCATTAGGATGAATTTTTAACTTCCCATATTACAGAACTAGTTTTTTCAGCTGCTGCTCTGTTGGCAATCAGCTCCAACCTTTTTGTTTCTGTAAATTCTTTGTAGGCTACACCATTGATTACAGGACTTGCTGTTGCTGCAGCAGCTTGTGCTGGCAGATATGGGATTCAGGCTTGGCAGGCATTCAAGGCAAGGCCACCAACGGCTCGACTGCGCAAGTTCTATGAAGGTGGCTTTCAGCCTACAATGACAAGGAGGGAAGCAGCTTTAATTCTAGGAATTAGGTATTTACTGAGTCTACAATGCGAGATGATCAAGCCCCTCATAACTCCATTAGTTTTCCTCCTATTTTTGTTGACAATTGACATTGGCTGTGTTGGAAATGTTTAAAAGCCCTTTTAAGATAGAAGATCATGCTTGCAATTTTTTCATATACATATGTGTGTGTGTTCGAGCTATTCATCCATCATGCTAATCTATAGTCTCTCTCTAATATGTCTCTGCTGTGCGTGCCTGTGGCTAAGATTTTATCTTCATTATATTGTCAGGGAGAGCACTCCAATGGAAAAGGTGAAAGAGGCGCACAGGAAGGTGATGGTTGCAAATCATCCAGATGCTGGTGGCAGCCATTATCTTGCTTCCAAGATCAACGAGGCGAAAGACGTCCTACTTGGGAAAACCAGGGGAAGCAATTCTGCATTCTGA

mRNA sequence

ATGGCTACACCATTGATTACAGGACTTGCTGTTGCTGCAGCAGCTTGTGCTGGCAGATATGGGATTCAGGCTTGGCAGGCATTCAAGGCAAGGCCACCAACGGCTCGACTGCGCAAGTTCTATGAAGGTGGCTTTCAGCCTACAATGACAAGGAGGGAAGCAGCTTTAATTCTAGGAATTAGGGAGAGCACTCCAATGGAAAAGGTGAAAGAGGCGCACAGGAAGGTGATGGTTGCAAATCATCCAGATGCTGGTGGCAGCCATTATCTTGCTTCCAAGATCAACGAGGCGAAAGACGTCCTACTTGGGAAAACCAGGGGAAGCAATTCTGCATTCTGA

Coding sequence (CDS)

ATGGCTACACCATTGATTACAGGACTTGCTGTTGCTGCAGCAGCTTGTGCTGGCAGATATGGGATTCAGGCTTGGCAGGCATTCAAGGCAAGGCCACCAACGGCTCGACTGCGCAAGTTCTATGAAGGTGGCTTTCAGCCTACAATGACAAGGAGGGAAGCAGCTTTAATTCTAGGAATTAGGGAGAGCACTCCAATGGAAAAGGTGAAAGAGGCGCACAGGAAGGTGATGGTTGCAAATCATCCAGATGCTGGTGGCAGCCATTATCTTGCTTCCAAGATCAACGAGGCGAAAGACGTCCTACTTGGGAAAACCAGGGGAAGCAATTCTGCATTCTGA

Protein sequence

MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGIRESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF
Homology
[Close]
BLAST of Lag0012599 vs. NCBI nr
Match: XP_022143712.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like [Momordica charantia])

HSP 1 Score: 214.5 bits (545), Expect = 4.5e-52
Identity = 107/112 (95.54%), Postives = 110/112 (98.21%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATPL+TGLAVAAAA AGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILG+
Sbjct: 1   MATPLVTGLAVAAAAYAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RESTP EKVKEAHRKVMVANHPDAGGSHYLASKINEAKD+LLGKTRGSNSAF
Sbjct: 61  RESTPTEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDILLGKTRGSNSAF 112

[Close]
BLAST of Lag0012599 vs. NCBI nr
Match: XP_022925536.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 [Cucurbita moschata] >XP_022978949.1 mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 [Cucurbita maxima] >XP_023543181.1 mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 [Cucurbita pepo subsp. pepo] >KAG6604441.1 Mitochondrial import inner membrane translocase subunit TIM14-1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034585.1 Mitochondrial import inner membrane translocase subunit TIM14-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 213.4 bits (542), Expect = 1.0e-51
Identity = 106/112 (94.64%), Postives = 110/112 (98.21%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATPLITG+AVAAAA AGRYG+QAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILG+
Sbjct: 1   MATPLITGIAVAAAAYAGRYGLQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RESTP EKVKEAHRKVMVANHPDAGGSHYLASKINEAKD+LLGKTRGSNSAF
Sbjct: 61  RESTPTEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDILLGKTRGSNSAF 112

[Close]
BLAST of Lag0012599 vs. NCBI nr
Match: XP_011653595.1 (mitochondrial import inner membrane translocase subunit TIM14-1 [Cucumis sativus] >KGN54184.1 hypothetical protein Csa_017970 [Cucumis sativus])

HSP 1 Score: 211.1 bits (536), Expect = 5.0e-51
Identity = 105/112 (93.75%), Postives = 109/112 (97.32%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATPLITG+AVAAAA AGRYGIQAWQAFK RPPTARLRKFYEGGFQPTMTRREAALILG+
Sbjct: 1   MATPLITGIAVAAAAYAGRYGIQAWQAFKMRPPTARLRKFYEGGFQPTMTRREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RESTP +KVKEAHRKVMVANHPDAGGSHYLASKINEAKD+LLGKTRGSNSAF
Sbjct: 61  RESTPTDKVKEAHRKVMVANHPDAGGSHYLASKINEAKDILLGKTRGSNSAF 112

[Close]
BLAST of Lag0012599 vs. NCBI nr
Match: XP_008449278.1 (PREDICTED: mitochondrial import inner membrane translocase subunit TIM14-1 [Cucumis melo] >KAA0047283.1 mitochondrial import inner membrane translocase subunit TIM14-1 [Cucumis melo var. makuwa])

HSP 1 Score: 209.9 bits (533), Expect = 1.1e-50
Identity = 105/112 (93.75%), Postives = 108/112 (96.43%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATPLITG+AVAAAA AGRYGIQAWQAFK RPPT RLRKFYEGGFQPTMTRREAALILGI
Sbjct: 1   MATPLITGIAVAAAAYAGRYGIQAWQAFKMRPPTVRLRKFYEGGFQPTMTRREAALILGI 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RESTP +KVKEAHRKVMVANHPDAGGSHYLASKINEAKD+LLGKTRGSNSAF
Sbjct: 61  RESTPTDKVKEAHRKVMVANHPDAGGSHYLASKINEAKDILLGKTRGSNSAF 112

[Close]
BLAST of Lag0012599 vs. NCBI nr
Match: XP_021656851.1 (mitochondrial import inner membrane translocase subunit TIM14-3-like [Hevea brasiliensis])

HSP 1 Score: 201.8 bits (512), Expect = 3.0e-48
Identity = 99/112 (88.39%), Postives = 107/112 (95.54%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATPLITG+A+AAAA AGRYGIQAWQAFKARPPTAR+R+FYEGGFQP MTRREAALILGI
Sbjct: 1   MATPLITGMAIAAAAYAGRYGIQAWQAFKARPPTARMRRFYEGGFQPVMTRREAALILGI 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RESTP +KVKEAHR+VMVANHPDAGGSHYLASKINEAKD+LLGK +GS SAF
Sbjct: 61  RESTPSDKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMLLGKAKGSGSAF 112

[Close]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match: Q8RV04 (Mitochondrial import inner membrane translocase subunit TIM14-1 OS=Arabidopsis thaliana OX=3702 GN=TIM14-1 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 6.0e-47
Identity = 92/112 (82.14%), Postives = 101/112 (90.18%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATP I G+AVAA A AGRYGIQAWQAFKARPP  +++KFYEGGFQPTMT+REAALILG+
Sbjct: 1   MATPFIAGVAVAATALAGRYGIQAWQAFKARPPRPKIKKFYEGGFQPTMTKREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RES   EKVKEAHRKVMVANHPDAGGSH+LASKINEAKDV+LGKT+ S SAF
Sbjct: 61  RESVAAEKVKEAHRKVMVANHPDAGGSHFLASKINEAKDVMLGKTKNSGSAF 112

[Close]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match: Q9LYY2 (Mitochondrial import inner membrane translocase subunit TIM14-3 OS=Arabidopsis thaliana OX=3702 GN=TIM14-3 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.4e-43
Identity = 88/112 (78.57%), Postives = 98/112 (87.50%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           MATP+I G AVAAAA AGRYGI AWQAFKARP   R+R+FYEGGFQ +MTRREAALILG+
Sbjct: 1   MATPMIAGAAVAAAAVAGRYGILAWQAFKARPRVPRMRRFYEGGFQSSMTRREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RES   +KVKEAHR+VMVANHPDAGGSHYLASKINEAKD++LGK+  S SAF
Sbjct: 61  RESVVADKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKSNNSGSAF 112

[Close]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match: Q9SF33 (Mitochondrial import inner membrane translocase subunit TIM14-2 OS=Arabidopsis thaliana OX=3702 GN=TIM14-2 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.6e-42
Identity = 86/112 (76.79%), Postives = 95/112 (84.82%), Query Frame = 0 

Query: 1   MATPLITGLAVAAAACAGRYGIQAWQAFKARPPTARLRKFYEGGFQPTMTRREAALILGI 60
           M   +I G AVAAAA AG+YGI+AWQAFK RP   R+RKFYEGGFQ TM RREAALILG+
Sbjct: 1   MVAAIIAGAAVAAAAYAGKYGIEAWQAFKLRPVRPRMRKFYEGGFQATMNRREAALILGV 60

Query: 61  RESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTRGSNSAF 113
           RES   EKVKEAHR+VMVANHPDAGGSHYLASKINEAKD++LGKT+ S SAF
Sbjct: 61  RESVAAEKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKTKNSGSAF 112

[Close]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match: Q3ZBN8 (Mitochondrial import inner membrane translocase subunit TIM14 OS=Bos taurus OX=9913 GN=DNAJC19 PE=3 SV=3)

HSP 1 Score: 108.6 bits (270), Expect = 4.6e-23
Identity = 55/113 (48.67%), Postives = 77/113 (68.14%), Query Frame = 0 

Query: 2   ATPLITGLAVAAAACAGRYGIQAW--------QAFKARPPTARLRKFYEGGFQPTMTRRE 61
           +T +  GL +AAA  AGRY +QA         Q F++ P TA    +Y GGF+P MT+RE
Sbjct: 3   STVVAVGLTIAAAGFAGRYALQAMKHMEPQVKQVFQSLPKTAFSGGYYRGGFEPKMTKRE 62

Query: 62  AALILGIRESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTR 107
           AALILG+  +    K+++AHR++M+ NHPD GGS Y+A+KINEAKD+L G+ +
Sbjct: 63  AALILGVSPTANKAKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

[Close]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match: Q96DA6 (Mitochondrial import inner membrane translocase subunit TIM14 OS=Homo sapiens OX=9606 GN=DNAJC19 PE=1 SV=3)

HSP 1 Score: 105.5 bits (262), Expect = 3.9e-22
Identity = 54/113 (47.79%), Postives = 77/113 (68.14%), Query Frame = 0 

Query: 2   ATPLITGLAVAAAACAGRYGIQAW--------QAFKARPPTARLRKFYEGGFQPTMTRRE 61
           +T +  GL +AAA  AGRY +QA         Q F++ P +A    +Y GGF+P MT+RE
Sbjct: 3   STVVAVGLTIAAAGFAGRYVLQAMKHMEPQVKQVFQSLPKSAFSGGYYRGGFEPKMTKRE 62

Query: 62  AALILGIRESTPMEKVKEAHRKVMVANHPDAGGSHYLASKINEAKDVLLGKTR 107
           AALILG+  +    K+++AHR++M+ NHPD GGS Y+A+KINEAKD+L G+ +
Sbjct: 63  AALILGVSPTANKGKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

The following BLAST results are available for this feature:
BLAST of Lag0012599 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022143712.14.5e-5295.54mitochondrial import inner membrane translocase subunit TIM14-1-like [Momordica ... [more]
XP_022925536.11.0e-5194.64mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 ... [more]
XP_011653595.15.0e-5193.75mitochondrial import inner membrane translocase subunit TIM14-1 [Cucumis sativus... [more]
XP_008449278.11.1e-5093.75PREDICTED: mitochondrial import inner membrane translocase subunit TIM14-1 [Cucu... [more]
XP_021656851.13.0e-4888.39mitochondrial import inner membrane translocase subunit TIM14-3-like [Hevea bras... [more]
BLAST of Lag0012599 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RV046.0e-4782.14Mitochondrial import inner membrane translocase subunit TIM14-1 OS=Arabidopsis t... [more]
Q9LYY21.4e-4378.57Mitochondrial import inner membrane translocase subunit TIM14-3 OS=Arabidopsis t... [more]
Q9SF332.6e-4276.79Mitochondrial import inner membrane translocase subunit TIM14-2 OS=Arabidopsis t... [more]
Q3ZBN84.6e-2348.67Mitochondrial import inner membrane translocase subunit TIM14 OS=Bos taurus OX=9... [more]
Q96DA63.9e-2247.79Mitochondrial import inner membrane translocase subunit TIM14 OS=Homo sapiens OX... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 52..106
e-value: 0.001
score: 28.3
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 53..112
score: 8.885875
IPR001623DnaJ domainCDDcd06257DnaJcoord: 57..101
e-value: 7.38706E-7
score: 40.9917
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 39..105
e-value: 1.7E-28
score: 100.3
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 43..104
NoneNo IPR availablePANTHERPTHR12763UNCHARACTERIZEDcoord: 1..112
NoneNo IPR availablePANTHERPTHR12763:SF51MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM14-3coord: 1..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012599.1Lag0012599.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0001405 PAM complex, Tim23 associated import motor
molecular_function GO:0001671 ATPase activator activity