Lag0012569 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0012569 vs. NCBI nr
Match: XP_038891595.1 (uncharacterized protein LOC120080986 [Benincasa hispida])

HSP 1 Score: 109.4 bits (272), Expect = 4.4e-20
Identity = 58/122 (47.54%), Postives = 74/122 (60.66%), Query Frame = 0

Query: 13  KSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQLVIERLQN---- 72
           KSE LGIH+ D E +WL ++FG+K+ + P TYLGLPLGGN   + FWQLV+ER+Q+    
Sbjct: 15  KSELLGIHVLDSEFDWLVNSFGFKRDYEPFTYLGLPLGGNPNHVTFWQLVMERIQHKLHN 74

Query: 73  -----LLISTSVIL----FWEGSRGEGGMHNVNWATTQLPQLLGGNDIDDLGPNAHVNGL 122
                L+IS    L    FWE S  + G+HNVNW  +Q PQLLGG  I +        GL
Sbjct: 75  WKWFPLVISCLDKLVQDFFWEDSHDDVGIHNVNWEISQRPQLLGGLGIGNFKHRNSTLGL 134

BLAST of Lag0012569 vs. NCBI nr
Match: RVW15386.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-17
Identity = 75/256 (29.30%), Postives = 103/256 (40.23%), Query Frame = 0

Query: 40  WPSTYLGLPLGGNSKAIHFWQLVIER-LQNLLISTSVI--------------LFWEGSRG 99
           WP +YLGLPLGGN K I FW  V+ER L++ ++S   I                W G+  
Sbjct: 168 WPLSYLGLPLGGNPKTIGFWDPVVERILRSYVLSLFKIPVSIASKIENLQRDFQWSGAGK 227

Query: 100 EGGMHNVNWATTQLPQLLGG--------NDIDDLGP---------------------NAH 159
               H + W     P+ LGG         +I  LG                        H
Sbjct: 228 GKKDHLIRWDVVCRPKKLGGLGFGKTSLRNIALLGKWLWRFPRERNSLWHKVIASIYGIH 287

Query: 160 VNG---------LHRVIWTNLY-----PKIKIFLWELSLGAINTANRLQKHMPHFQLSPS 219
            NG          HR  W  L+      K+K   W +  G +NT N+LQ   P+  L P 
Sbjct: 288 PNGWDANMVDRWSHRCPWKFLWSLKAPSKVKDLAWLVVHGKVNTNNKLQLRRPYKSLCPQ 347

Query: 220 WCIMCAASSEHPEHLFFHCTFASRYWAMILDGFGWSTVIPNSIQDSLPLIFMGHPFSWRK 238
            CI+C  + E  +HLF HC      W  + +  G + V P SI D + + F G   S R 
Sbjct: 348 LCILCKGNGESIDHLFLHCPVTIGLWHKLFNLAGLAWVPPRSIVDMMVIAFKGLGNSLRG 407

BLAST of Lag0012569 vs. NCBI nr
Match: RVW45746.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-17
Identity = 74/253 (29.25%), Postives = 105/253 (41.50%), Query Frame = 0

Query: 2   VSRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQL 61
           +S L +N    KS   GI+LD   ++ L  T   K   WP  YLGLPLGGN +A  FW  
Sbjct: 313 ISGLKVNLD--KSNIYGINLDQAHISRLAETLECKASGWPILYLGLPLGGNPRAGGFWDP 372

Query: 62  VIERLQNLLISTSVILFWEGS--RGEGGMHNV-----NWATTQLPQLLGGNDIDDLGPNA 121
           VIER+   L        W+ +  R E   H +           L + L G  +    P+A
Sbjct: 373 VIERISRRLDG------WQKAYLRREDNSHPILSYPYALDLEGLMRSLDGLHLSPSVPDA 432

Query: 122 HV-----NGLHRV---------------------IWTNLYP-KIKIFLWELSLGAINTAN 181
                  +GL  V                     +W +  P K++ F+W ++   +NT +
Sbjct: 433 RFWPLSSSGLFSVKSFFLALSQFSGSPQVFPSKFVWNSQVPFKVQSFIWLVAHKKVNTND 492

Query: 182 RLQKHMPHFQLSPSWCIMCAASSEHPEHLFFHCTFASRYWAMILDGFGWSTVIPNSIQDS 221
            LQ   P+  LSP  CI+C    E  +H+F HC+     W  +        V P SI D 
Sbjct: 493 MLQVRRPYKALSPDICILCMKHGESADHIFLHCSLTIGLWHRLFQLAKMDWVPPRSILDM 552

BLAST of Lag0012569 vs. NCBI nr
Match: KAA0044451.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-16
Identity = 78/304 (25.66%), Postives = 115/304 (37.83%), Query Frame = 0

Query: 3   SRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQLV 62
           S LNIN +  KS    I++       +  ++G  +GH P++YLG+PLGG   + +FW  V
Sbjct: 105 SGLNINLS--KSTIFPINVPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNV 164

Query: 63  IERLQNLLIS-------------------------------------------------- 122
           ++++Q  L S                                                  
Sbjct: 165 LQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLS 224

Query: 123 --------------TSVILFWEGSRGEGGM--------------HNVNWA-TTQLPQ--- 182
                          SV  FW  S  +  +              HN+  +  T LP    
Sbjct: 225 LVVPRLFALSTNKKGSVKDFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGL 284

Query: 183 -----LLGGNDIDDLG-------------PNAHVNGLHRVIWTNLYP-KIKIFLWELSLG 206
                 L  N+I D                N H N L++ +W   +P K K F+W L  G
Sbjct: 285 PKPLWKLNSNNIFDTASVKKILSEAPVSPANFHPN-LYKTLWKVEFPKKCKFFIWTLIHG 344

BLAST of Lag0012569 vs. NCBI nr
Match: TYK29578.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-16
Identity = 78/304 (25.66%), Postives = 115/304 (37.83%), Query Frame = 0

Query: 3   SRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQLV 62
           S LNIN +  KS    I++       +  ++G  +GH P++YLG+PLGG   + +FW  V
Sbjct: 62  SGLNINLS--KSTIFPINVPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNV 121

Query: 63  IERLQNLLIS-------------------------------------------------- 122
           ++++Q  L S                                                  
Sbjct: 122 LQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLS 181

Query: 123 --------------TSVILFWEGSRGEGGM--------------HNVNWA-TTQLPQ--- 182
                          SV  FW  S  +  +              HN+  +  T LP    
Sbjct: 182 LVVPRLFALSTNKKGSVKDFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGL 241

Query: 183 -----LLGGNDIDDLG-------------PNAHVNGLHRVIWTNLYP-KIKIFLWELSLG 206
                 L  N+I D                N H N L++ +W   +P K K F+W L  G
Sbjct: 242 PKPLWKLNSNNIFDTASVKKILSEAPVSPANFHPN-LYKTLWKVEFPKKCKFFIWTLIHG 301

BLAST of Lag0012569 vs. ExPASy TrEMBL
Match: A0A438BWM3 (Putative ribonuclease H protein OS=Vitis vinifera OX=29760 GN=VvCHDh000004_604 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.7e-17
Identity = 75/256 (29.30%), Postives = 103/256 (40.23%), Query Frame = 0

Query: 40  WPSTYLGLPLGGNSKAIHFWQLVIER-LQNLLISTSVI--------------LFWEGSRG 99
           WP +YLGLPLGGN K I FW  V+ER L++ ++S   I                W G+  
Sbjct: 168 WPLSYLGLPLGGNPKTIGFWDPVVERILRSYVLSLFKIPVSIASKIENLQRDFQWSGAGK 227

Query: 100 EGGMHNVNWATTQLPQLLGG--------NDIDDLGP---------------------NAH 159
               H + W     P+ LGG         +I  LG                        H
Sbjct: 228 GKKDHLIRWDVVCRPKKLGGLGFGKTSLRNIALLGKWLWRFPRERNSLWHKVIASIYGIH 287

Query: 160 VNG---------LHRVIWTNLY-----PKIKIFLWELSLGAINTANRLQKHMPHFQLSPS 219
            NG          HR  W  L+      K+K   W +  G +NT N+LQ   P+  L P 
Sbjct: 288 PNGWDANMVDRWSHRCPWKFLWSLKAPSKVKDLAWLVVHGKVNTNNKLQLRRPYKSLCPQ 347

Query: 220 WCIMCAASSEHPEHLFFHCTFASRYWAMILDGFGWSTVIPNSIQDSLPLIFMGHPFSWRK 238
            CI+C  + E  +HLF HC      W  + +  G + V P SI D + + F G   S R 
Sbjct: 348 LCILCKGNGESIDHLFLHCPVTIGLWHKLFNLAGLAWVPPRSIVDMMVIAFKGLGNSLRG 407

BLAST of Lag0012569 vs. ExPASy TrEMBL
Match: A0A438ED36 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_239 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.7e-17
Identity = 74/253 (29.25%), Postives = 105/253 (41.50%), Query Frame = 0

Query: 2   VSRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQL 61
           +S L +N    KS   GI+LD   ++ L  T   K   WP  YLGLPLGGN +A  FW  
Sbjct: 313 ISGLKVNLD--KSNIYGINLDQAHISRLAETLECKASGWPILYLGLPLGGNPRAGGFWDP 372

Query: 62  VIERLQNLLISTSVILFWEGS--RGEGGMHNV-----NWATTQLPQLLGGNDIDDLGPNA 121
           VIER+   L        W+ +  R E   H +           L + L G  +    P+A
Sbjct: 373 VIERISRRLDG------WQKAYLRREDNSHPILSYPYALDLEGLMRSLDGLHLSPSVPDA 432

Query: 122 HV-----NGLHRV---------------------IWTNLYP-KIKIFLWELSLGAINTAN 181
                  +GL  V                     +W +  P K++ F+W ++   +NT +
Sbjct: 433 RFWPLSSSGLFSVKSFFLALSQFSGSPQVFPSKFVWNSQVPFKVQSFIWLVAHKKVNTND 492

Query: 182 RLQKHMPHFQLSPSWCIMCAASSEHPEHLFFHCTFASRYWAMILDGFGWSTVIPNSIQDS 221
            LQ   P+  LSP  CI+C    E  +H+F HC+     W  +        V P SI D 
Sbjct: 493 MLQVRRPYKALSPDICILCMKHGESADHIFLHCSLTIGLWHRLFQLAKMDWVPPRSILDM 552

BLAST of Lag0012569 vs. ExPASy TrEMBL
Match: A0A5A7TSA7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001840 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 8.4e-17
Identity = 78/304 (25.66%), Postives = 115/304 (37.83%), Query Frame = 0

Query: 3   SRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQLV 62
           S LNIN +  KS    I++       +  ++G  +GH P++YLG+PLGG   + +FW  V
Sbjct: 105 SGLNINLS--KSTIFPINVPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNV 164

Query: 63  IERLQNLLIS-------------------------------------------------- 122
           ++++Q  L S                                                  
Sbjct: 165 LQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLS 224

Query: 123 --------------TSVILFWEGSRGEGGM--------------HNVNWA-TTQLPQ--- 182
                          SV  FW  S  +  +              HN+  +  T LP    
Sbjct: 225 LVVPRLFALSTNKKGSVKDFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGL 284

Query: 183 -----LLGGNDIDDLG-------------PNAHVNGLHRVIWTNLYP-KIKIFLWELSLG 206
                 L  N+I D                N H N L++ +W   +P K K F+W L  G
Sbjct: 285 PKPLWKLNSNNIFDTASVKKILSEAPVSPANFHPN-LYKTLWKVEFPKKCKFFIWTLIHG 344

BLAST of Lag0012569 vs. ExPASy TrEMBL
Match: A0A5D3E1E3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001840 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 8.4e-17
Identity = 78/304 (25.66%), Postives = 115/304 (37.83%), Query Frame = 0

Query: 3   SRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQLV 62
           S LNIN +  KS    I++       +  ++G  +GH P++YLG+PLGG   + +FW  V
Sbjct: 62  SGLNINLS--KSTIFPINVPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNV 121

Query: 63  IERLQNLLIS-------------------------------------------------- 122
           ++++Q  L S                                                  
Sbjct: 122 LQKIQKKLSSWKYSQLSKGGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLS 181

Query: 123 --------------TSVILFWEGSRGEGGM--------------HNVNWA-TTQLPQ--- 182
                          SV  FW  S  +  +              HN+  +  T LP    
Sbjct: 182 LVVPRLFALSTNKKGSVKDFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGL 241

Query: 183 -----LLGGNDIDDLG-------------PNAHVNGLHRVIWTNLYP-KIKIFLWELSLG 206
                 L  N+I D                N H N L++ +W   +P K K F+W L  G
Sbjct: 242 PKPLWKLNSNNIFDTASVKKILSEAPVSPANFHPN-LYKTLWKVEFPKKCKFFIWTLIHG 301

BLAST of Lag0012569 vs. ExPASy TrEMBL
Match: A0A803QGT5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 9.3e-16
Identity = 76/319 (23.82%), Postives = 118/319 (36.99%), Query Frame = 0

Query: 2    VSRLNINYAKVKSEFLGIHLDDLELNWLTSTFGYKQGHWPSTYLGLPLGGNSKAIHFWQL 61
            +S L +N +  K + LG+ +D+L ++   +  G + G WP  YLG+PL G+ +   FW L
Sbjct: 1107 LSGLKVNLS--KRQLLGLCMDELVVSQCAAQIGCEVGCWPMKYLGMPLAGSPRKKVFWDL 1166

Query: 62   VIER-------LQN----------------------------LLISTSV-----ILFWE- 121
            V+E+       L N                             L+   V     I FWE 
Sbjct: 1167 VLEKVIESRYGLANNFWDSKRGERMSPRGPWKDIADLYDEYLRLVKFKVGKGDKIRFWED 1226

Query: 122  ------------------------------GSRGEGGMHNVNW--------ATTQLPQLL 181
                                             G  G   V+W           ++P L+
Sbjct: 1227 EWLGDSSLLHTYPGLALISRAKNVYIIELVAEGGAAGESVVSWDFKFRRNLLEKEIPSLI 1286

Query: 182  G-------------GNDIDDLGPNA--------------HVNGLHRVIWTN------LYP 209
            G               DI    P+A                  + R  WT          
Sbjct: 1287 GMLQQMEQVKLLNVNVDIRIWKPDATGVFSSKSAFAWFNSNQNVARPPWTKSLWKSIALS 1346

BLAST of Lag0012569 vs. TAIR 10
Match: AT4G04650.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 50.4 bits (119), Expect = 2.3e-06
Identity = 26/80 (32.50%), Postives = 41/80 (51.25%), Query Frame = 0

Query: 110 LGPNAHVNGLHRVIW-TNLYPKIKIFLWELSLGAINTANRLQKHMPHFQLS-PSWCIMCA 169
           L P +H    H+ +W  N  PK     W ++   ++T +RLQ    ++ LS P+ C++C 
Sbjct: 120 LHPQSHTVPWHKAVWFKNHVPKHAFICWVVAWNRLHTRDRLQ----NWGLSIPAECLLCN 179

Query: 170 ASSEHPEHLFFHCTFASRYW 188
           A  +   HLFF C F+   W
Sbjct: 180 AHDDSRAHLFFECQFSGVVW 195

BLAST of Lag0012569 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.5e-05
Identity = 24/80 (30.00%), Postives = 39/80 (48.75%), Query Frame = 0

Query: 110 LGPNAHVNGLHRVIW-TNLYPKIKIFLWELSLGAINTANRLQKHMPHFQLS-PSWCIMCA 169
           L P  H+   ++ +W  N  PK     W ++   ++T +RL+     + LS P+ C++C 
Sbjct: 142 LHPQNHIVPWYKAVWFKNHVPKHAFICWVVAWNRLHTRDRLRS----WGLSIPAVCLLCN 201

Query: 170 ASSEHPEHLFFHCTFASRYW 188
           +  E   HLFF C F    W
Sbjct: 202 SHDESRAHLFFECPFCGAVW 217