Lag0012511 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0012511
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Sphingolipid delta(4)-desaturase DES1-like
Location	chr1: 41765960 .. 41768177 (-)
RNA-Seq Expression	Lag0012511
Synteny	Lag0012511

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCACCGTCACCGTTTTGATGGCGGCGCTGCAACGAATGGGGACTCTCTACATGCGAGGTACTCGAGATCGAGTTTCCAACGGGCGATCCCAAGGTCGACAGAGTCGGGGGCATTAAATGGAGAGCGGAGTTTGGGATTAGCTGCGTGATTTTGATTTTTTGAGAGATTAGGGATTTGGATGATCGGGCGGCGATGTCGGAGGATTACGATTTCATCGGAGGGATAGAATTTTAAAGCCACCCAAATTTCTATAAGTGATAGAAATTTTTCTATCATAGAATCTATCAGTGATAGAAAAGTTTCTAATTTTTCTATCACTGATAGAATCTATCAGTGATAGAAAAGTTTCTACAAGTGATAGAATTAACTTTCTATCACTGATAGAAAGTTGATTGTCAGTGATAGAAGATTTCTATCACTGATAGAAAATTTTCTATCAGTGATAGAAAAATTTTCTATCACTGATAGAAATCTTCGATAGAATTATGACTTCTATCACTGATAGAAACCTTTCTATCCGTGATAGAAGATTTCTATCATTGATGTGATAGATTCTATCAGTGATAGAAAGTTTTTCTATCAATGATAGAAAATTCTATCACTGATAGAAAGTTTCTATCAGTGATAGAAAGTTTTTCTATCACTGATAGATTCTATCAGTGATAGAAAAACTTTCTATCAGTGATACAAAACTTCTATCACTAATAGAAAGTTAATTCTATCAATGATAGAAGATTTCTATCACTGATAGAAAGTTGATAGCATTAACTTTCTATCAGTGATAGAAGATTTCTATCAGTGATAGAAAACTTTTCTATCAACTGATAGAAACTTTTCTATCACTGATAGACAAATTTTCTATTAGTGATAGAAATCTTCGATAGAATTATGATTTTTATCACTGATAGAAACCTTTCTATCAGTGATAGAAGATTTCTATCATTGATGTGATAGATTCTATCAGTGATGTGATAGATTCTATCAGTGATAGAAATTTTCTATCAGTGATAGAAAATTCTATCACTGATAGGAAAGTTAACTCTATCACTGATAGAAAGTTCTATCACAACTTTCCTATCACTGATAGAAAGTTTCTATCAGTGATAGAATCTATCACTGATAGATTCTATCAGTGATAGAAAAACTTTCTATCAGTGATACAAAACTTCTATCACTAATAGAAAGTTAATTCTATCAATGATAGAAGATTTCTATCACTGATAGAAAGTTGATAGAATTAACTTTCTATCAGTGATAGAAGATTTCTATCAATGATAGAAAATTTTTCTATCAGTGATAGAAAACTTTTCTATCACTGATAGAATCTATCAGTGATAGAAAAATTTTCTATCAGTGATAGAAATCTTCGATAGAATTATGATTTCTATCACTGATAAAAACATTTCTATCAGTGATAGAAGATTTATATCATTGATGTGATAGATTCTATTAGTGATAGAAAGTTTTTCTATCAATGATAGAAAGTTTTGTATCACTGATAGAAAGTTTTGTATCACTGATAGAACGTTTTTCTATCTCTGATATAACGTTTTTCTATCTCTGATAGATTCTATCAGTGATAGAAAAACGTTCTATCACTGATAGATTCTATCAGTGATAGAAAAGCGTTCTATCAGTGATAGAAATCTTCTATCATTGATAGAAAGTTTTCTATCACTGATAGAAAGTTAATTCTATAAGTGATAGAAAACTTCTATCACTGATAGATTCTATCAGTGATAGAAAAATTTTCTATCATATAGAAAGTTGAAAAATTTTCTATCAAATAGACACCTACCAAAACAAAAATAGAGGAGAAAAATCCGAGCCGAACCGAAGCTCATGTAGTGACAAATTTGTTCTCAAAAGCCATATGGGTCATTTTCCAACTCTTCTTCTATGCGTTTCGGCCTCTGTTTCTCAAACCAAAGCCTCCAGGCTACTGGGAGTTCATAAACTTCACCATCTCTTGATGTGGCTATAGTTTGCCTTTGGGGCTGGAAGTCTTTTGCTTATCTGATCCTTTCCACATTTGTTGGAGGAGGTATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTCTTCAACCCCGACCAGGAGACGTATTCGTACTACGGTCCGCTCAATCTTCTTACATGGAGCGTTGGTTACCACAATGAGCATCATGATTTCCCCAGGATTTCTATCACTGATAGATTCTATCAGTGA

mRNA sequence

ATGACCACCGTCACCGTTTTGATGGCGGCGCTGCAACGAATGGGGACTCTCTACATGCGAGTTTGCCTTTGGGGCTGGAAGTCTTTTGCTTATCTGATCCTTTCCACATTTGTTGGAGGAGGTATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTCTTCAACCCCGACCAGGAGACGTATTCGTACTACGGTCCGCTCAATCTTCTTACATGGAGCGTTGGTTACCACAATGAGCATCATGATTTCCCCAGGATTTCTATCACTGATAGATTCTATCAGTGA

Coding sequence (CDS)

ATGACCACCGTCACCGTTTTGATGGCGGCGCTGCAACGAATGGGGACTCTCTACATGCGAGTTTGCCTTTGGGGCTGGAAGTCTTTTGCTTATCTGATCCTTTCCACATTTGTTGGAGGAGGTATGCATCCAATGGCTGGTCACTTCATTTCAGAACATTATGTCTTCAACCCCGACCAGGAGACGTATTCGTACTACGGTCCGCTCAATCTTCTTACATGGAGCGTTGGTTACCACAATGAGCATCATGATTTCCCCAGGATTTCTATCACTGATAGATTCTATCAGTGA

Protein sequence

MTTVTVLMAALQRMGTLYMRVCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHNEHHDFPRISITDRFYQ

Homology

[Close]

BLAST of Lag0012511 vs. NCBI nr
Match: XP_042058107.1 (sphingolipid delta(4)-desaturase DES1-like [Salvia splendens] >KAG6423662.1 hypothetical protein SASPL_114064 [Salvia splendens])

HSP 1 Score: 150.6 bits (379), Expect = 6.9e-33
Identity = 65/68 (95.59%), Postives = 68/68 (100.00%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           VCL+GWKSF+YLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNL+TWSVGYHN
Sbjct: 203 VCLFGWKSFSYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLMTWSVGYHN 262

Query: 81  EHHDFPRI 89
           EHHDFPRI
Sbjct: 263 EHHDFPRI 270

[Close]

BLAST of Lag0012511 vs. NCBI nr
Match: KAF8413900.1 (hypothetical protein HHK36_001896 [Tetracentron sinense])

HSP 1 Score: 150.6 bits (379), Expect = 6.9e-33
Identity = 64/72 (88.89%), Postives = 68/72 (94.44%), Query Frame = 0


Query: 18  YMRVCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVG 77
           Y  V  WGWKSFAY+ILSTF+GGGMHPMAGHFISEHY+FNPDQETYSYYGPLNLLTWSVG
Sbjct: 202 YAMVYFWGWKSFAYMILSTFLGGGMHPMAGHFISEHYIFNPDQETYSYYGPLNLLTWSVG 261

Query: 78  YHNEHHDFPRIS 90
           YHNEHHDFPRI+
Sbjct: 262 YHNEHHDFPRIA 273

[Close]

BLAST of Lag0012511 vs. NCBI nr
Match: XP_022723947.1 (sphingolipid delta(4)-desaturase DES1-like [Durio zibethinus])

HSP 1 Score: 149.8 bits (377), Expect = 1.2e-32
Identity = 66/68 (97.06%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           V LWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVF PDQETYSYYGPLNLLTWSVGYHN
Sbjct: 208 VYLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFKPDQETYSYYGPLNLLTWSVGYHN 267

Query: 81  EHHDFPRI 89
           EHHDFPRI
Sbjct: 268 EHHDFPRI 275

[Close]

BLAST of Lag0012511 vs. NCBI nr
Match: XP_034698823.1 (sphingolipid delta(4)-desaturase DES1-like [Vitis riparia] >XP_034698824.1 sphingolipid delta(4)-desaturase DES1-like [Vitis riparia])

HSP 1 Score: 149.1 bits (375), Expect = 2.0e-32
Identity = 65/68 (95.59%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           V  WGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP+QETYSYYGPLNLLTWSVGYHN
Sbjct: 204 VYFWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPEQETYSYYGPLNLLTWSVGYHN 263

Query: 81  EHHDFPRI 89
           EHHDFPRI
Sbjct: 264 EHHDFPRI 271

[Close]

BLAST of Lag0012511 vs. NCBI nr
Match: CAN81291.1 (hypothetical protein VITISV_005313 [Vitis vinifera])

HSP 1 Score: 149.1 bits (375), Expect = 2.0e-32
Identity = 65/68 (95.59%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           V  WGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP+QETYSYYGPLNLLTWSVGYHN
Sbjct: 194 VYFWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPEQETYSYYGPLNLLTWSVGYHN 253

Query: 81  EHHDFPRI 89
           EHHDFPRI
Sbjct: 254 EHHDFPRI 261

[Close]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 8.5e-34
Identity = 63/68 (92.65%), Postives = 66/68 (97.06%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           V  +GW+SFAYLILSTFVGGGMHPMAGHFISEHYVFNP+QETYSYYGPLNLLTWSVGYHN
Sbjct: 213 VLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVFNPNQETYSYYGPLNLLTWSVGYHN 272

Query: 81  EHHDFPRI 89
           EHHDFPRI
Sbjct: 273 EHHDFPRI 280

[Close]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 3.2e-33
Identity = 65/76 (85.53%), Postives = 68/76 (89.47%), Query Frame = 0


Query: 21  VCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHN 80
           V  +GWKS AYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNL+TW VGYHN
Sbjct: 208 VYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLMTWHVGYHN 267

Query: 81  EHHDFPRISITDRFYQ 97
           EHHDFPRI  T R Y+
Sbjct: 268 EHHDFPRIPGT-RLYK 282

[Close]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot
Match: Q5AJX2 (Sphingolipid delta(4)-desaturase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=DES1 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.6e-22
Identity = 48/93 (51.61%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 18  YMRVCLWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQ----------------- 77
           ++ V  WGWK+  Y I+STF+ G +HP AGHFI+EHYV N +                  
Sbjct: 219 HVMVTCWGWKALGYFIVSTFLAGSLHPCAGHFIAEHYVLNENNVPRHQKIGGTNISKELL 278

Query: 78  --ETYSYYGPLNLLTWSVGYHNEHHDFPRISIT 92
             ETYSYYG LN+LTW+VGYHNEHHDFP I+ T
Sbjct: 279 PAETYSYYGTLNMLTWNVGYHNEHHDFPYIAWT 311

[Close]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot
Match: Q5F3C1 (Sphingolipid delta(4)-desaturase DES1 OS=Gallus gallus OX=9031 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-21
Identity = 43/66 (65.15%), Postives = 54/66 (81.82%), Query Frame = 0


Query: 23  LWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHNEH 82
           LWG KS  Y++  + +G G+HP++GHFI+EHY+F    ETYSYYGPLNLLT++VGYHNEH
Sbjct: 203 LWGAKSIFYMLAGSVLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEH 262

Query: 83  HDFPRI 89
           HDFP I
Sbjct: 263 HDFPNI 268

[Close]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot
Match: O09005 (Sphingolipid delta(4)-desaturase DES1 OS=Mus musculus OX=10090 GN=Degs1 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.2e-21
Identity = 41/66 (62.12%), Postives = 55/66 (83.33%), Query Frame = 0


Query: 23  LWGWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPDQETYSYYGPLNLLTWSVGYHNEH 82
           ++G KS  Y++ +T +G G+HP++GHFI+EHY+F    ETYSYYGPLNLLT++VGYHNEH
Sbjct: 203 VFGVKSLVYMLAATLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEH 262

Query: 83  HDFPRI 89
           HDFP +
Sbjct: 263 HDFPNV 268

The following BLAST results are available for this feature:

BLAST of Lag0012511 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_042058107.1	6.9e-33	95.59	sphingolipid delta(4)-desaturase DES1-like [Salvia splendens] >KAG6423662.1 hypo...	[more]
KAF8413900.1	6.9e-33	88.89	hypothetical protein HHK36_001896 [Tetracentron sinense]	[more]
XP_022723947.1	1.2e-32	97.06	sphingolipid delta(4)-desaturase DES1-like [Durio zibethinus]	[more]
XP_034698823.1	2.0e-32	95.59	sphingolipid delta(4)-desaturase DES1-like [Vitis riparia] >XP_034698824.1 sphin...	[more]
CAN81291.1	2.0e-32	95.59	hypothetical protein VITISV_005313 [Vitis vinifera]	[more]

BLAST of Lag0012511 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZPH4	8.5e-34	92.65	Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q6H5U3	3.2e-33	85.53	Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39...	[more]
Q5AJX2	2.6e-22	51.61	Sphingolipid delta(4)-desaturase OS=Candida albicans (strain SC5314 / ATCC MYA-2...	[more]
Q5F3C1	1.7e-21	65.15	Sphingolipid delta(4)-desaturase DES1 OS=Gallus gallus OX=9031 GN=DEGS1 PE=1 SV=...	[more]
O09005	8.2e-21	62.12	Sphingolipid delta(4)-desaturase DES1 OS=Mus musculus OX=10090 GN=Degs1 PE=1 SV=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 6..89 e-value: 1.2E-8 score: 35.2
None	No IPR available	PANTHER	PTHR12879	SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2	coord: 21..91
None	No IPR available	PANTHER	PTHR12879:SF17	SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKE	coord: 21..91

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0012511.1	Lag0012511.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046513	ceramide biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042284	sphingolipid delta-4 desaturase activity