Lag0012339 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012339
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPyruvate decarboxylase
Locationchr1: 40134398 .. 40137623 (-)
RNA-Seq ExpressionLag0012339
SyntenyLag0012339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCACCCCCTCAACTTTGGGCCACTACCTTGCTCGTAGGCTCCTTCAAATCGGTGTCTCGGATATCTTTTCGGTTCCGGGGGACTCCAATCTTGTGCTGCTGGATTACTTTGTGGCAGAGCAAGGGCTGAAGCTTGTGGGGTGTTGTAGCGAGCTCAATGCCGGCTATGCGGCCGATGGCTACGCCAGGCGTCGAGGCGTTGGAGCCTGCGCTGTGACATTCACTGTTGGTGGGCTCAGCCTCATCAACGCCATTGCTGGCGCTTACTCTGAGGATCTTCCAGTGATTTGCATCGTGGGTGGACCAAACTCGAATTATTATGGGAGTAAGAAGATTATCCACCATACCATTGGCTTGCCGGATTTCACTCAAGAACTTCGCTGCTTTCAAAATGTCACGTGTCATCAGGCCATTATCGATAATTTGGAAGATGCACAATGGAAGATAGATACAGCAATATCAAAGTGTTTGGAAGAGAGCAAACCAGTTTACATTAGTATTTCTTCCAATTTGGTCGCCATTCCTCATCCTTCTTTCTCCATCCAACCACTCGTTCCACTCTCCCTTTCTCCCAAGTAATTTCTCATTTCAAACCCCAACACAATAAATTAAAAAAGATATATTTAAGATCTTTCCTTTTTAAAATCCTTATTCTCTCTGCCGAATGATTTCACAGGCAAACTAACCAAATGGGTCTGGAGATGGCAGTGGAGAAGACAGCTCAGCTTCTAAACAACGCCGTAAAACCAGTCATGGTCGGCGGGAAGAAGCTGCGACCCGCCAAAGCACAGGCGGCGTTTGTCGAGCTAGCCGACGCTTGTGGCTACGCCGTCGCCGTTACCCCGTCGGCGAAGGGTATGTTCCCGGAGAACCACCCTCATTTCATCGGAACTTACTGGGGAACAGTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATAGCTGACGGTTCCATCTTCGTCGGAGCCACGTTGGACGAATTGGAGACTGTCGCCTTCTCTCTAACCTACAAAAAGAACAAGGCCATTATCGTAGAACTAGATCGCGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTCGGGCCCTAGCTAAGCGGGTAAAGCCCAATACAACGGCTTACGAGAATTATGGTCGGATTTATATTCCGGAGTCGGGTCCAGTCCAATCGGAGCCGGGCGAGGCATTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTAAGCAATAACATGGCTGTGATATCTGAGACGGGTGACTCTTGGTTCCAATGTCAAAAACTCAAATTACCAGACTCATGCGGGTAAAATTTTGTTATATGATATCCATGATCATAAGTTCAGAGAACAAAAAATACGTCCGGTTTTTAATTCTTTAAAAAAAAATAATAATTTAGGGTGTGTTTGATTTAACTTTTCAAGTGTTTAATTTTGAAAAAAAAAAATTTGAAAGAAATTGTAATGTTTGACAACCACTAAAAATGACTTATTAAAAAATGAGATTGAGAAATAAACCATTTTGAGAGAAACACTCGAAATCAAATTTTTTAAAAAGAGTTTGTAAGTATATAGTAACTTGTGAATAAAAAAACTTACGAGTAAATCTAACCAAACATATGAGTGTTTAAATTCTAAACAGCTTTTATACAAAATATTTAAAAGAAAATGCGAGTGGTATATAACTTCGTATATTAGGTGTGGTTTACAGTACTTTAATTCAAGTTAATATTTTATTTAGAACATCGAAACAAACCAAAAATTCCAAATTTAAAAAAACCAAATACAAAATAATTATTTTGAATATTTTATTTTAAAATTTTTATTTTCTTAATTTTTAATTAAAAATTTTAGAGAACAAAATATTAGAAAATTTATTAAGCTAGGCTTGTGTTAACACCACAATCCTTTGATCAAAAAAAAAAAAAAAAAAAAACCACAATCCTAACTTGATTTCATTATTATGTGACATAATATCTGCATTACGAGGGGACATTTTGACAAATTTTACCTTAAGAACTTAAATTAAATTGTTTATAATATGGAAATGACTTTGAAAATTTGTAGGTATGAGATACAATTGCTATATTCATCAATTGGATGGTCTCTAGGTGCAGCCCTGGGATATGCACAAGGTGCATCAGAGGAGCGAGTGGTACTATGCATAGGTGATGGGAGTTTTCAGGTCAATTACCATATATATTTCTCTTTGGCCTTTAATATCATCGGAAATGTTCTATCTTGATCTCTAACTTTCAATGGTGATCAATCTCACTTGTATACCAAGTGCATAAACTACCATCAAAATTTTAGAATAACCATTAAATACAAAATTTTAGAGATCAAGTAGAATTATCAAACTCATTTATATATATTATCAAACTTTATTTTCTTTTTTGAAAGATATGAAATGATTATGTTATGCAGATGGCTCCACAAGATGTGTCAACAATGTTAAGATTGGGGCAAAATAATATAATATTTTTGATAAATAACGGTGGATATACCATTGAAGTAGAGATTCACGATGGTCCTTATAATATCATCAAGAATTGGGACTACACTGCCCTGGTCAATGCCTTGCACAATGGTGATGGAAAATGTTGGACAGCTAAGGTGAAATCTCAATATCCATTTTCCTTTTTATGTATATATAAGCTATCTAAAAATAATTTTTAAAAATTTTAATACAACCAAACATGCTCCACTGAGTTTTCATCTTAAAAAAAAATAGACCATTAATTTGAAAAGAGTTTCAAACAAGCATAAGAACGTGTTTGGAATGATTTTTTAAATGTTTAAAAACATTTCTTTTATACTTAAAAGTACTTGAAAGTCATTTCAAACATGTTTTAAATTTTTTTGGCTGGTTAACCATCAATCTAAAATATTCTACTTTTTTTTCAATGAAAATGTTTAGGAAAAAAAAAAGAATAAGTAAAAATGTATTAGTCAAAAGAGATAGGGTATTTAAAAAACATTTGCATACTTTTGAACGTTTTTATAGATTTTCGAGTTTTGACATATAGGTTCAAACAGAAGAAGAGTTGATCAATGCAATTAAAACAGCAATGGAAGATCAAAATGATTGTTTGTGCTTCATAGAGGCCATTGTTCATAGAGATGATACAAGTAAAGAATTACTTGAATTTGGGAATAAGATTGCTGCTATTAGTAGCCGTCCTCCCAAACCTTTATGA

mRNA sequence

ATGGCCACCACCCCCTCAACTTTGGGCCACTACCTTGCTCGTAGGCTCCTTCAAATCGGTGTCTCGGATATCTTTTCGGTTCCGGGGGACTCCAATCTTGTGCTGCTGGATTACTTTGTGGCAGAGCAAGGGCTGAAGCTTGTGGGGTGTTGTAGCGAGCTCAATGCCGGCTATGCGGCCGATGGCTACGCCAGGCGTCGAGGCGTTGGAGCCTGCGCTGTGACATTCACTGTTGGTGGGCTCAGCCTCATCAACGCCATTGCTGGCGCTTACTCTGAGGATCTTCCAGTGATTTGCATCGTGGGTGGACCAAACTCGAATTATTATGGGAGTAAGAAGATTATCCACCATACCATTGGCTTGCCGGATTTCACTCAAGAACTTCGCTGCTTTCAAAATGTCACGTGTCATCAGGCCATTATCGATAATTTGGAAGATGCACAATGGAAGATAGATACAGCAATATCAAAGTGTTTGGAAGAGAGCAAACCAGTTTACATTAGTATTTCTTCCAATTTGGTCGCCATTCCTCATCCTTCTTTCTCCATCCAACCACTCGTTCCACTCTCCCTTTCTCCCAAGCAAACTAACCAAATGGGTCTGGAGATGGCAGTGGAGAAGACAGCTCAGCTTCTAAACAACGCCGTAAAACCAGTCATGGTCGGCGGGAAGAAGCTGCGACCCGCCAAAGCACAGGCGGCGTTTGTCGAGCTAGCCGACGCTTGTGGCTACGCCGTCGCCGTTACCCCGTCGGCGAAGGGTATGTTCCCGGAGAACCACCCTCATTTCATCGGAACTTACTGGGGAACAGTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATAGCTGACGGTTCCATCTTCGTCGGAGCCACGTTGGACGAATTGGAGACTGTCGCCTTCTCTCTAACCTACAAAAAGAACAAGGCCATTATCGTAGAACTAGATCGCGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTCGGGCCCTAGCTAAGCGGGTAAAGCCCAATACAACGGCTTACGAGAATTATGGTCGGATTTATATTCCGGAGTCGGGTCCAGTCCAATCGGAGCCGGGCGAGGCATTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTAAGCAATAACATGGCTGTGATATCTGAGACGGGTGACTCTTGGTTCCAATGTCAAAAACTCAAATTACCAGACTCATGCGGGTATGAGATACAATTGCTATATTCATCAATTGGATGGTCTCTAGGTGCAGCCCTGGGATATGCACAAGGTGCATCAGAGGAGCGAGTGGTACTATGCATAGGTGATGGGAGTTTTCAGATGGCTCCACAAGATGTGTCAACAATGTTAAGATTGGGGCAAAATAATATAATATTTTTGATAAATAACGGTGGATATACCATTGAAGTAGAGATTCACGATGGTCCTTATAATATCATCAAGAATTGGGACTACACTGCCCTGGTCAATGCCTTGCACAATGGTGATGGAAAATGTTGGACAGCTAAGGTTCAAACAGAAGAAGAGTTGATCAATGCAATTAAAACAGCAATGGAAGATCAAAATGATTGTTTGTGCTTCATAGAGGCCATTGTTCATAGAGATGATACAAGTAAAGAATTACTTGAATTTGGGAATAAGATTGCTGCTATTAGTAGCCGTCCTCCCAAACCTTTATGA

Coding sequence (CDS)

ATGGCCACCACCCCCTCAACTTTGGGCCACTACCTTGCTCGTAGGCTCCTTCAAATCGGTGTCTCGGATATCTTTTCGGTTCCGGGGGACTCCAATCTTGTGCTGCTGGATTACTTTGTGGCAGAGCAAGGGCTGAAGCTTGTGGGGTGTTGTAGCGAGCTCAATGCCGGCTATGCGGCCGATGGCTACGCCAGGCGTCGAGGCGTTGGAGCCTGCGCTGTGACATTCACTGTTGGTGGGCTCAGCCTCATCAACGCCATTGCTGGCGCTTACTCTGAGGATCTTCCAGTGATTTGCATCGTGGGTGGACCAAACTCGAATTATTATGGGAGTAAGAAGATTATCCACCATACCATTGGCTTGCCGGATTTCACTCAAGAACTTCGCTGCTTTCAAAATGTCACGTGTCATCAGGCCATTATCGATAATTTGGAAGATGCACAATGGAAGATAGATACAGCAATATCAAAGTGTTTGGAAGAGAGCAAACCAGTTTACATTAGTATTTCTTCCAATTTGGTCGCCATTCCTCATCCTTCTTTCTCCATCCAACCACTCGTTCCACTCTCCCTTTCTCCCAAGCAAACTAACCAAATGGGTCTGGAGATGGCAGTGGAGAAGACAGCTCAGCTTCTAAACAACGCCGTAAAACCAGTCATGGTCGGCGGGAAGAAGCTGCGACCCGCCAAAGCACAGGCGGCGTTTGTCGAGCTAGCCGACGCTTGTGGCTACGCCGTCGCCGTTACCCCGTCGGCGAAGGGTATGTTCCCGGAGAACCACCCTCATTTCATCGGAACTTACTGGGGAACAGTCAGCACCGCCTTCTGCGGCGAGACGGTGGAAATAGCTGACGGTTCCATCTTCGTCGGAGCCACGTTGGACGAATTGGAGACTGTCGCCTTCTCTCTAACCTACAAAAAGAACAAGGCCATTATCGTAGAACTAGATCGCGTTGTGTTTCCAAATGGGCCGAGTTTTGGGCCGATTTTAATGAAGGACTTTCTTCGGGCCCTAGCTAAGCGGGTAAAGCCCAATACAACGGCTTACGAGAATTATGGTCGGATTTATATTCCGGAGTCGGGTCCAGTCCAATCGGAGCCGGGCGAGGCATTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTAAGCAATAACATGGCTGTGATATCTGAGACGGGTGACTCTTGGTTCCAATGTCAAAAACTCAAATTACCAGACTCATGCGGGTATGAGATACAATTGCTATATTCATCAATTGGATGGTCTCTAGGTGCAGCCCTGGGATATGCACAAGGTGCATCAGAGGAGCGAGTGGTACTATGCATAGGTGATGGGAGTTTTCAGATGGCTCCACAAGATGTGTCAACAATGTTAAGATTGGGGCAAAATAATATAATATTTTTGATAAATAACGGTGGATATACCATTGAAGTAGAGATTCACGATGGTCCTTATAATATCATCAAGAATTGGGACTACACTGCCCTGGTCAATGCCTTGCACAATGGTGATGGAAAATGTTGGACAGCTAAGGTTCAAACAGAAGAAGAGTTGATCAATGCAATTAAAACAGCAATGGAAGATCAAAATGATTGTTTGTGCTTCATAGAGGCCATTGTTCATAGAGATGATACAAGTAAAGAATTACTTGAATTTGGGAATAAGATTGCTGCTATTAGTAGCCGTCCTCCCAAACCTTTATGA

Protein sequence

MATTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRDDTSKELLEFGNKIAAISSRPPKPL
Homology
BLAST of Lag0012339 vs. NCBI nr
Match: XP_022144001.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 1016.9 bits (2628), Expect = 6.7e-293
Identity = 490/565 (86.73%), Postives = 528/565 (93.45%), Query Frame = 0

Query: 5   PSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYA 64
           P TLGHYLARRL++IGV DIFSVPGD NL+LLDYFVAE GL LVGCCSELNAGYAADGYA
Sbjct: 42  PWTLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYA 101

Query: 65  RRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDF 124
           RRRGVGACAVTFTVGGLSLINA+AGAYSEDLPV+CIVGGPNSN YGSKKIIHHTIGLPDF
Sbjct: 102 RRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDF 161

Query: 125 TQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQ 184
           +QELRCFQNVTC+QAIIDN+E+ QW+ID AI KCL E KPVYISI  NLVAIPHPSFS++
Sbjct: 162 SQELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSME 221

Query: 185 PLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGY 244
            L+PLSL PKQ+NQMGLE+A EK AQLLN+AVKPVMV GKKLRPAKAQAAFVELADACGY
Sbjct: 222 RLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGY 281

Query: 245 AVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLT 304
           AVAVTPSAKG+FPENHPHFIGTYWG VSTAFCGETVEIAD SIF GATLDELETVAFSLT
Sbjct: 282 AVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLT 341

Query: 305 YKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQ 364
           YKKNKAIIVE +RVVFPNGPSFGPILMKDFLRALA+R+KPN TAYENY RIY+PESGP+Q
Sbjct: 342 YKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQ 401

Query: 365 SEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWS 424
           SEPGEALRVNVLFKHIQKMLS+NM VISE GDSWF CQKLKLP SCGYE+QLLY+SIGWS
Sbjct: 402 SEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWS 461

Query: 425 LGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHD 484
           LGA LGYAQ A ++RVVLCIGDGSFQM+PQDVSTMLRLGQ NIIFLINNGGYTIEVEIHD
Sbjct: 462 LGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHD 521

Query: 485 GPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHR 544
           GPYN+IKNWDYTA+VNA+HN DGKCWTAKV TEEEL+ AI+TA +D+NDCLCFIEAI+HR
Sbjct: 522 GPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHR 581

Query: 545 DDTSKELLEFGNKIAAISSRPPKPL 570
           DDTSKELLEFG+KIAAIS RPP+PL
Sbjct: 582 DDTSKELLEFGSKIAAISCRPPRPL 606

BLAST of Lag0012339 vs. NCBI nr
Match: XP_038881352.1 (pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1012.7 bits (2617), Expect = 1.3e-291
Identity = 489/565 (86.55%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLVL DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 58  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAERGLNLVGCCNELNAGYAADG 117

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 118 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 177

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF+QELRCFQNVTC+QAIIDNLEDAQW+ID AISKCLEE KPVYISI  NLVAIPHPSFS
Sbjct: 178 DFSQELRCFQNVTCYQAIIDNLEDAQWQIDRAISKCLEERKPVYISICCNLVAIPHPSFS 237

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            Q L+PLSLSPK++NQM LE+AVEK AQLLN+A+KPVM+GGKKLRPAKAQ AF+ELADAC
Sbjct: 238 AQQLIPLSLSPKKSNQMALEVAVEKAAQLLNSAIKPVMIGGKKLRPAKAQEAFMELADAC 297

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
            YAVAVTPSAKGMFPE HPHFIGTYWGTVSTA+CGETVEIAD SIF GATLDELETVAFS
Sbjct: 298 RYAVAVTPSAKGMFPEYHPHFIGTYWGTVSTAYCGETVEIADASIFAGATLDELETVAFS 357

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAIIVE DRVVFPNGPSFGPILMKDFL A+AKR+ PN TAYENY RIYI ESGP
Sbjct: 358 LTYKKNKAIIVEADRVVFPNGPSFGPILMKDFLSAIAKRLNPNPTAYENYRRIYIAESGP 417

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           +QS PGEALRVNVLFKHIQKMLS+N+ VISETGDSWFQCQKLKLP SCGYEIQLLY+SIG
Sbjct: 418 LQSNPGEALRVNVLFKHIQKMLSSNIVVISETGDSWFQCQKLKLPKSCGYEIQLLYASIG 477

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A E+R+VLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 478 WSLGATLGYAQAAPEKRMVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 537

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+VNA+HN DG CWT K+ TEEEL+NAI+ AM DQ DCLCFIE I+
Sbjct: 538 HDGPYNVIKNWDYTAIVNAMHNRDGNCWTTKIHTEEELVNAIEKAMGDQKDCLCFIEVII 597

Query: 543 HRDDTSKELLEFGNKIAAISSRPPK 568
           HRDDTSKELLEFG+K AAISSRPP+
Sbjct: 598 HRDDTSKELLEFGSKFAAISSRPPR 622

BLAST of Lag0012339 vs. NCBI nr
Match: TYK29657.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.7e-289
Identity = 481/567 (84.83%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLV+ DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 75  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGYAADG 134

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 135 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 194

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF QELRCFQNVTC+QAIIDNLEDAQWKIDTAISKCLEE KP+YISI  NLVAIPHPSFS
Sbjct: 195 DFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPHPSFS 254

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            QPL+PLSLSPKQ+NQM LE AVEK AQLLN A+KPVM+GGKKLRP K+Q  F+ELA+AC
Sbjct: 255 AQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIELANAC 314

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY VAVTPSAKGMFPENHPHFIG YWGTVS+A+CGETVEIAD SIFVGATLDELETVAF+
Sbjct: 315 GYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELETVAFT 374

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAI+VE DRVVFPNGPSFGPILMKDFL ALAKR+KPN+TAYENY RIYI ESGP
Sbjct: 375 LTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIAESGP 434

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++S PGEALRVN+LFKHIQKMLS+NM VISE GDSWF CQ+LKLP SCGYE+QLLY+SIG
Sbjct: 435 LESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLYASIG 494

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A EERVVLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 495 WSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 554

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+V A+HN DG CWT KV TEEEL+NAI+  +E++ D LCFIE IV
Sbjct: 555 HDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFIEVIV 614

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKPL 570
           HRDDTS+ELLEFG+K AA+SSRPP+PL
Sbjct: 615 HRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Lag0012339 vs. NCBI nr
Match: XP_008464213.1 (PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037741.1 pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.9e-288
Identity = 480/567 (84.66%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLV+ DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 75  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGYAADG 134

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 135 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 194

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF QELRCFQNVTC+QAIIDNLEDAQWKIDTAISKCLEE KP+YISI  NLVAIPHPSFS
Sbjct: 195 DFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPHPSFS 254

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            QPL+PLSLSPKQ+NQM LE AVEK AQLLN A+KPVM+GGKKLRP K+Q  F+ELA+AC
Sbjct: 255 AQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIELANAC 314

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY VAVTPSAKGMFPE+HPHFIG YWGTVS+A+CGETVEIAD SIFVGATLDELETVAF+
Sbjct: 315 GYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELETVAFT 374

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAI+VE DRVVFPNGPSFGPILMKDFL ALAKR+KPN+TAYENY RIYI ESGP
Sbjct: 375 LTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIAESGP 434

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++S PGEALRVN+LFKHIQKMLS+NM VISE GDSWF CQ+LKLP SCGYE+QLLY+SIG
Sbjct: 435 LESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLYASIG 494

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A EERVVLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 495 WSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 554

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+V A+HN DG CWT KV TEEEL+NAI+  +E++ D LCFIE IV
Sbjct: 555 HDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFIEVIV 614

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKPL 570
           HRDDTS+ELLEFG+K AA+SSRPP+PL
Sbjct: 615 HRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Lag0012339 vs. NCBI nr
Match: XP_023544035.1 (pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992.6 bits (2565), Expect = 1.4e-285
Identity = 479/564 (84.93%), Postives = 519/564 (92.02%), Query Frame = 0

Query: 4   TPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGY 63
           TPSTLGHYLARRL+QIGVSDIFSVPGDSNLVLLDYFVAE+GL LVGCCSELNAGYAADGY
Sbjct: 61  TPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 64  ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPD 123
           ARRRGVGAC VTFTVGGLSLINAIAGAYSEDLP++CIVG PNSN YGS KI+HHTIGLPD
Sbjct: 121 ARRRGVGACVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPD 180

Query: 124 FTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSI 183
           F+QELRCFQNVTC+QA+I+NLEDAQW+IDTAISKCLE+SKPVYISIS NL AIPHPSFS+
Sbjct: 181 FSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSM 240

Query: 184 QPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACG 243
           +  +PLS+S KQTNQMGLEMAVE  AQLLN AVKPVMVGGKKLR AKAQAAFVELAD C 
Sbjct: 241 KQFLPLSISSKQTNQMGLEMAVEMAAQLLNTAVKPVMVGGKKLRVAKAQAAFVELADVCD 300

Query: 244 YAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSL 303
           YAVAVTPSAKGMFPENH HFIGTYWG++S+AFCGETVEIAD SIF GA  DELETV +SL
Sbjct: 301 YAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSL 360

Query: 304 TYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPV 363
           +YKKNKAIIVE DRVVFPNGPSFGPILMKDFLRALAKR+KPN TAYENY RIYIP+SGP 
Sbjct: 361 SYKKNKAIIVESDRVVFPNGPSFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPT 420

Query: 364 QSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGW 423
           QSEPGEALRVNVLFKHIQKMLS NMAVISE GDSWF CQKL+LP SCG+E+QL+Y+SIGW
Sbjct: 421 QSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGW 480

Query: 424 SLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIH 483
           SLGA LGYAQ + E+RVVLCIGDGSFQMAPQD+STMLRLGQ NIIFLINNGGYTIEVEIH
Sbjct: 481 SLGATLGYAQASPEDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIH 540

Query: 484 DGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVH 543
           DGPYN+IKNWDYT LV A+HN  G CWT KV+TEEEL+ AI+ A  D+N+CLCFIEAIVH
Sbjct: 541 DGPYNVIKNWDYTGLVEAMHNQTGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVH 600

Query: 544 RDDTSKELLEFGNKIAAISSRPPK 568
           RDDTSKELL+FGNK+AA +SRPPK
Sbjct: 601 RDDTSKELLDFGNKLAATNSRPPK 624

BLAST of Lag0012339 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 826.2 bits (2133), Expect = 2.2e-238
Identity = 388/563 (68.92%), Postives = 469/563 (83.30%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           ++LG +LARRL+Q+GVSD+F+VPGD NL LLD+ +AE GL+LVGCC+ELNAGYAADGYAR
Sbjct: 44  ASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYAR 103

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGACAVTFTVGGLS++NAIAGAYSE+LPVICI GGPNSN YG+ +I+HHTIGLPDF+
Sbjct: 104 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFS 163

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTCHQA++ NLEDA  +IDTAI+  L ESKPVY+SIS NL  +PHP+FS  P
Sbjct: 164 QELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDP 223

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP  L+P+ +N+MGLE AVE T + LN AVKPV+VGG KLR AKA  AFV+L DA GYA
Sbjct: 224 -VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYA 283

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
            AV PSAKG+ PE HPHFIGTYWG VSTAFC E VE AD  +F G   ++  +V +S   
Sbjct: 284 YAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLL 343

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK+KAIIV+ +RV+  NGP+FG ++MK+FL  LAKRV  NTTAYENY RI++PE  P++S
Sbjct: 344 KKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLES 403

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
           EP E LRVNVLFKH+QKML+++ AVI+ETGDSWF CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 404 EPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 463

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQGA ++RV+ CIGDGSFQ+  QDVSTM+R  QN+IIFLINNGGYTIEVEIHDG
Sbjct: 464 GALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDG 523

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YT LV+A+HNG+GKCWT+KV+ EEEL  AI  A+ ++ DCLCFIE I H+D
Sbjct: 524 PYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIEVIAHKD 583

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 584 DTSKELLEWGSRVSAANSRPPNP 605

BLAST of Lag0012339 vs. ExPASy Swiss-Prot
Match: Q10MW3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 5.4e-237
Identity = 388/563 (68.92%), Postives = 468/563 (83.13%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           ++LG +LARRL+Q+GVSD+F+VPGD NL LLD+ +AE GL+LVGCC+ELNAGYAADGYAR
Sbjct: 44  ASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYAR 103

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGACAVTFTVGGLS++NAIAGAYSE+LPVICI GGPNSN YG+ +I+HHTIGLPDF+
Sbjct: 104 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFS 163

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTCHQA++ NLEDA  +IDTAI+  L ESKPVY+SIS NL  +PHP+FS  P
Sbjct: 164 QELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDP 223

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP  L+P+ +N+MGLE AVE T + LN AVKPV+VGG KLR AKA  AFV+L DA GYA
Sbjct: 224 -VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYA 283

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
            AV PSAKG+ PE HPHFIGTYWG VSTAFC E VE AD  +F G   ++  +V +S   
Sbjct: 284 YAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLL 343

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK+KAIIV+ +RV+  NGP+FG ++MK+FL  LAKRV  NTTAYENY RI++PE  P++S
Sbjct: 344 KKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLES 403

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
           EP E LRVNVLFKH+QKML+++ AVI+ETGDSWF CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 404 EPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 463

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQGA ++RV+ CIGDGSFQ+  QDVSTM+R  QN+IIFLINNGGYTIEVEIHDG
Sbjct: 464 GALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDG 523

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YT LV+A+HNG+GKCWT+KV+ EEEL  AI  A+  + DCLCFIE I H+D
Sbjct: 524 PYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMAL-GEKDCLCFIEVIAHKD 583

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 584 DTSKELLEWGSRVSAANSRPPNP 604

BLAST of Lag0012339 vs. ExPASy Swiss-Prot
Match: Q9FFT4 (Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 8.1e-233
Identity = 387/563 (68.74%), Postives = 456/563 (80.99%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           +TLG YLARRL++IGV+D+FSVPGD NL LLD+ +AE  LKL+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGAC VTFTVGGLS++NAIAGAYSE+LP+ICIVGGPNSN YG+ +I+HHTIGLPDFT
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFT 164

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTC QA+I+NLE+A   IDTAIS  L+ESKPVYISIS NL AIP P+FS  P
Sbjct: 165 QELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRHP 224

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP  L  K +NQ+GL+ AVE  A+ LN AVKPV+VGG K+R AKA  AFVELADA GY 
Sbjct: 225 -VPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYG 284

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
           +AV PSAKG  PE+H HFIGTYWG VSTAFC E VE AD  +F G   ++  +V +SL  
Sbjct: 285 LAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK KAIIV+ DRV   NGP+FG +LMKDFL  LAKR+K N T+YENY RIY+PE  P++ 
Sbjct: 345 KKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD 404

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
            P E+LRVNVLF+HIQ MLS+  AV++ETGDSWF CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 405 NPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 464

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQ     RV+ CIGDGSFQ+  QDVSTM+R GQ  IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YTA V A+HNG+GKCWTAKV+ EEEL+ AI TA  ++ +  CFIE IVH+D
Sbjct: 525 PYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKD 584

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of Lag0012339 vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 8.1e-233
Identity = 386/566 (68.20%), Postives = 458/566 (80.92%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           T+ STLG +L+RRL+Q GV+D+FSVPGD NL LLD+ +AE  L  +GCC+ELNAGYAADG
Sbjct: 38  TSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADG 97

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YAR RGVGAC VTFTVGGLS++NAIAGAYSE+LPVICIVGGPNSN +G+ +I+HHTIGLP
Sbjct: 98  YARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLP 157

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF+QELRCFQ VTC+QA+++NLEDA  +ID AI+  L+ESKPVYISIS NL A PHP+F+
Sbjct: 158 DFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFA 217

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
             P VP  L+P+ +N MGLE AVE T + LN AVKPVMVGG KLR AKA  AF+ELADA 
Sbjct: 218 RDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADAS 277

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY +AV PS KG+ PENHPHFIGTYWG VST FC E VE AD  IF G   ++  +V +S
Sbjct: 278 GYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYS 337

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           L  KK KAIIV  DRVV  NGP+FG +LM DF R LAKRVK N TAYENY RI++PE  P
Sbjct: 338 LLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKP 397

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++ +PGE LRVN +F+HIQKMLS+  AVI+ETGDSWF CQKLKLP  CGYE Q+ Y SIG
Sbjct: 398 LKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIG 457

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WS+GA LGYAQ   E+RV+  IGDGSFQ+  QD+STM+R GQ  IIFLINNGGYTIEVEI
Sbjct: 458 WSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEI 517

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNW+YT LV+A+HNG+GKCWT KV+ EEEL+ AIKTA  ++ D LCFIE IV
Sbjct: 518 HDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIV 577

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKP 569
           H+DDTSKELLE+G++++A + RPP P
Sbjct: 578 HKDDTSKELLEWGSRVSAANGRPPNP 602

BLAST of Lag0012339 vs. ExPASy Swiss-Prot
Match: O82647 (Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 5.2e-232
Identity = 385/563 (68.38%), Postives = 460/563 (81.71%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           +TLG +LARRL+Q GV+D+FSVPGD NL LLD+ +AE  L L+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGAC VTFTVGGLS++NAIAGAYSE+LP+ICIVGGPNSN YG+ +I+HHTIGLPDF+
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTC+QA+++NL+DA  +ID AIS  L+ESKPVYIS+S NL AIPH +FS  P
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP 224

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP SL+P+ +N+MGLE AVE T + LN AVKPVMVGG KLR AKA  AFVELADA GYA
Sbjct: 225 -VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
           +A+ PSAKG  PE+HPHFIGTYWG VST FC E VE AD  IF G   ++  +V +SL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK KAI+V+ DR+   NGP+FG ILM DF R L+KRVK N TAYENY RI++PE  P++ 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
           E  E LRVN +F+HIQKMLS+  AVI+ETGDSWF CQKLKLP  CGYE Q+ Y SIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQ + E+RV+  IGDGSFQ+  QD+STMLR GQ  IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YT LV+A+HNG+G CWTAKV+ EEEL+ AI TA  ++ DCLCFIE I+H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of Lag0012339 vs. ExPASy TrEMBL
Match: A0A6J1CS04 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.2e-293
Identity = 490/565 (86.73%), Postives = 528/565 (93.45%), Query Frame = 0

Query: 5   PSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYA 64
           P TLGHYLARRL++IGV DIFSVPGD NL+LLDYFVAE GL LVGCCSELNAGYAADGYA
Sbjct: 42  PWTLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYA 101

Query: 65  RRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDF 124
           RRRGVGACAVTFTVGGLSLINA+AGAYSEDLPV+CIVGGPNSN YGSKKIIHHTIGLPDF
Sbjct: 102 RRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDF 161

Query: 125 TQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQ 184
           +QELRCFQNVTC+QAIIDN+E+ QW+ID AI KCL E KPVYISI  NLVAIPHPSFS++
Sbjct: 162 SQELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSME 221

Query: 185 PLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGY 244
            L+PLSL PKQ+NQMGLE+A EK AQLLN+AVKPVMV GKKLRPAKAQAAFVELADACGY
Sbjct: 222 RLIPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGY 281

Query: 245 AVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLT 304
           AVAVTPSAKG+FPENHPHFIGTYWG VSTAFCGETVEIAD SIF GATLDELETVAFSLT
Sbjct: 282 AVAVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLT 341

Query: 305 YKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQ 364
           YKKNKAIIVE +RVVFPNGPSFGPILMKDFLRALA+R+KPN TAYENY RIY+PESGP+Q
Sbjct: 342 YKKNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQ 401

Query: 365 SEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWS 424
           SEPGEALRVNVLFKHIQKMLS+NM VISE GDSWF CQKLKLP SCGYE+QLLY+SIGWS
Sbjct: 402 SEPGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWS 461

Query: 425 LGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHD 484
           LGA LGYAQ A ++RVVLCIGDGSFQM+PQDVSTMLRLGQ NIIFLINNGGYTIEVEIHD
Sbjct: 462 LGATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHD 521

Query: 485 GPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHR 544
           GPYN+IKNWDYTA+VNA+HN DGKCWTAKV TEEEL+ AI+TA +D+NDCLCFIEAI+HR
Sbjct: 522 GPYNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHR 581

Query: 545 DDTSKELLEFGNKIAAISSRPPKPL 570
           DDTSKELLEFG+KIAAIS RPP+PL
Sbjct: 582 DDTSKELLEFGSKIAAISCRPPRPL 606

BLAST of Lag0012339 vs. ExPASy TrEMBL
Match: A0A5D3E0N5 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold669G00060 PE=3 SV=1)

HSP 1 Score: 1003.4 bits (2593), Expect = 3.7e-289
Identity = 481/567 (84.83%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLV+ DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 75  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGYAADG 134

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 135 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 194

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF QELRCFQNVTC+QAIIDNLEDAQWKIDTAISKCLEE KP+YISI  NLVAIPHPSFS
Sbjct: 195 DFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPHPSFS 254

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            QPL+PLSLSPKQ+NQM LE AVEK AQLLN A+KPVM+GGKKLRP K+Q  F+ELA+AC
Sbjct: 255 AQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIELANAC 314

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY VAVTPSAKGMFPENHPHFIG YWGTVS+A+CGETVEIAD SIFVGATLDELETVAF+
Sbjct: 315 GYPVAVTPSAKGMFPENHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELETVAFT 374

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAI+VE DRVVFPNGPSFGPILMKDFL ALAKR+KPN+TAYENY RIYI ESGP
Sbjct: 375 LTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIAESGP 434

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++S PGEALRVN+LFKHIQKMLS+NM VISE GDSWF CQ+LKLP SCGYE+QLLY+SIG
Sbjct: 435 LESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLYASIG 494

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A EERVVLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 495 WSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 554

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+V A+HN DG CWT KV TEEEL+NAI+  +E++ D LCFIE IV
Sbjct: 555 HDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFIEVIV 614

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKPL 570
           HRDDTS+ELLEFG+K AA+SSRPP+PL
Sbjct: 615 HRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Lag0012339 vs. ExPASy TrEMBL
Match: A0A5A7T827 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00370 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 480/567 (84.66%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLV+ DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 75  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGYAADG 134

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 135 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 194

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF QELRCFQNVTC+QAIIDNLEDAQWKIDTAISKCLEE KP+YISI  NLVAIPHPSFS
Sbjct: 195 DFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPHPSFS 254

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            QPL+PLSLSPKQ+NQM LE AVEK AQLLN A+KPVM+GGKKLRP K+Q  F+ELA+AC
Sbjct: 255 AQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIELANAC 314

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY VAVTPSAKGMFPE+HPHFIG YWGTVS+A+CGETVEIAD SIFVGATLDELETVAF+
Sbjct: 315 GYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELETVAFT 374

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAI+VE DRVVFPNGPSFGPILMKDFL ALAKR+KPN+TAYENY RIYI ESGP
Sbjct: 375 LTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIAESGP 434

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++S PGEALRVN+LFKHIQKMLS+NM VISE GDSWF CQ+LKLP SCGYE+QLLY+SIG
Sbjct: 435 LESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLYASIG 494

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A EERVVLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 495 WSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 554

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+V A+HN DG CWT KV TEEEL+NAI+  +E++ D LCFIE IV
Sbjct: 555 HDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFIEVIV 614

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKPL 570
           HRDDTS+ELLEFG+K AA+SSRPP+PL
Sbjct: 615 HRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Lag0012339 vs. ExPASy TrEMBL
Match: A0A1S3CKZ0 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502148 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 480/567 (84.66%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           TTPSTLGHYLARRL+QIG+SDIFSVPGDSNLV+ DYFVAE+GL LVGCC+ELNAGYAADG
Sbjct: 75  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVMFDYFVAEKGLNLVGCCNELNAGYAADG 134

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YARRRGVGACAVTFTVG LSLINAIAGAYSEDLPVICIVGGPNSN YGSKKI+HHTIGLP
Sbjct: 135 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 194

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF QELRCFQNVTC+QAIIDNLEDAQWKIDTAISKCLEE KP+YISI  NLVAIPHPSFS
Sbjct: 195 DFGQELRCFQNVTCYQAIIDNLEDAQWKIDTAISKCLEEGKPIYISICCNLVAIPHPSFS 254

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
            QPL+PLSLSPKQ+NQM LE AVEK AQLLN A+KPVM+GGKKLRP K+Q  F+ELA+AC
Sbjct: 255 AQPLIPLSLSPKQSNQMALEAAVEKAAQLLNTAIKPVMIGGKKLRPTKSQEPFIELANAC 314

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY VAVTPSAKGMFPE+HPHFIG YWGTVS+A+CGETVEIAD SIFVGATLDELETVAF+
Sbjct: 315 GYPVAVTPSAKGMFPESHPHFIGIYWGTVSSAYCGETVEIADASIFVGATLDELETVAFT 374

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           LTYKKNKAI+VE DRVVFPNGPSFGPILMKDFL ALAKR+KPN+TAYENY RIYI ESGP
Sbjct: 375 LTYKKNKAIMVEADRVVFPNGPSFGPILMKDFLWALAKRLKPNSTAYENYRRIYIAESGP 434

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++S PGEALRVN+LFKHIQKMLS+NM VISE GDSWF CQ+LKLP SCGYE+QLLY+SIG
Sbjct: 435 LESGPGEALRVNILFKHIQKMLSSNMVVISEAGDSWFHCQRLKLPKSCGYEVQLLYASIG 494

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WSLGA LGYAQ A EERVVLCIGDGSFQM PQDVSTM+ L QNNIIFLINNGGYTIEVEI
Sbjct: 495 WSLGATLGYAQAAPEERVVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 554

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNWDYTA+V A+HN DG CWT KV TEEEL+NAI+  +E++ D LCFIE IV
Sbjct: 555 HDGPYNVIKNWDYTAIVKAMHNHDGYCWTTKVHTEEELMNAIEIVLEERKDYLCFIEVIV 614

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKPL 570
           HRDDTS+ELLEFG+K AA+SSRPP+PL
Sbjct: 615 HRDDTSRELLEFGSKFAAVSSRPPRPL 641

BLAST of Lag0012339 vs. ExPASy TrEMBL
Match: A0A6J1IP68 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1)

HSP 1 Score: 989.6 bits (2557), Expect = 5.5e-285
Identity = 475/564 (84.22%), Postives = 520/564 (92.20%), Query Frame = 0

Query: 4   TPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGY 63
           TPSTLGHYLARRL+QIGVSDIFSVPGDSNLVLLDYFVAE+GL LVGCCSELNAGYAADGY
Sbjct: 42  TPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 101

Query: 64  ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPD 123
           ARRRGVG+C VTFTVGGLSLINAIAGAYSEDLP++CIVG PNSN YGS KI+HHTIGLPD
Sbjct: 102 ARRRGVGSCVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPD 161

Query: 124 FTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSI 183
           F+QELRCFQNVTC+QA+I+NLEDAQW+IDTAISKCLE+SKPVYISIS NL AIPHPSFS+
Sbjct: 162 FSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSM 221

Query: 184 QPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACG 243
           +  +PLS+S KQTNQMGLEMAVE  AQ+LN AVKP+MVGGKKLR AKAQAAFVELAD C 
Sbjct: 222 KQFLPLSISSKQTNQMGLEMAVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCD 281

Query: 244 YAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSL 303
           YAVAVTPSAKGMFPENH HFIGTYWG++S+AFCGETVEIAD SIF GA  DELETV +SL
Sbjct: 282 YAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSL 341

Query: 304 TYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPV 363
           +YKKNKAIIVE DRVVFPNGP FGPILMKDFLRALAKR+KPN TAYENY RIYIP+SGP 
Sbjct: 342 SYKKNKAIIVESDRVVFPNGPIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPT 401

Query: 364 QSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGW 423
           QSEPGEALRVNVLFKHIQKMLS NMAVISE GDSWF CQKL+LP SCG+E+QL+Y+SIGW
Sbjct: 402 QSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGW 461

Query: 424 SLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIH 483
           SLGA LGYAQ +S++RVVLCIGDGSFQMAPQD+STMLRLGQ NIIFLINNGGYTIEVEIH
Sbjct: 462 SLGATLGYAQASSDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIH 521

Query: 484 DGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVH 543
           DGPYN+IKNWDYT LV A+HN +G CWT KV+TEEEL+ AI+ A  D+N+CLCFIEAIVH
Sbjct: 522 DGPYNVIKNWDYTGLVEAMHNQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVH 581

Query: 544 RDDTSKELLEFGNKIAAISSRPPK 568
           RDDTSKELL+FGNK+AA +SRPPK
Sbjct: 582 RDDTSKELLDFGNKLAATNSRPPK 605

BLAST of Lag0012339 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 807.7 bits (2085), Expect = 5.7e-234
Identity = 386/566 (68.20%), Postives = 458/566 (80.92%), Query Frame = 0

Query: 3   TTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADG 62
           T+ STLG +L+RRL+Q GV+D+FSVPGD NL LLD+ +AE  L  +GCC+ELNAGYAADG
Sbjct: 38  TSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADG 97

Query: 63  YARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLP 122
           YAR RGVGAC VTFTVGGLS++NAIAGAYSE+LPVICIVGGPNSN +G+ +I+HHTIGLP
Sbjct: 98  YARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLP 157

Query: 123 DFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFS 182
           DF+QELRCFQ VTC+QA+++NLEDA  +ID AI+  L+ESKPVYISIS NL A PHP+F+
Sbjct: 158 DFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFA 217

Query: 183 IQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADAC 242
             P VP  L+P+ +N MGLE AVE T + LN AVKPVMVGG KLR AKA  AF+ELADA 
Sbjct: 218 RDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADAS 277

Query: 243 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFS 302
           GY +AV PS KG+ PENHPHFIGTYWG VST FC E VE AD  IF G   ++  +V +S
Sbjct: 278 GYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYS 337

Query: 303 LTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGP 362
           L  KK KAIIV  DRVV  NGP+FG +LM DF R LAKRVK N TAYENY RI++PE  P
Sbjct: 338 LLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKP 397

Query: 363 VQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIG 422
           ++ +PGE LRVN +F+HIQKMLS+  AVI+ETGDSWF CQKLKLP  CGYE Q+ Y SIG
Sbjct: 398 LKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIG 457

Query: 423 WSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEI 482
           WS+GA LGYAQ   E+RV+  IGDGSFQ+  QD+STM+R GQ  IIFLINNGGYTIEVEI
Sbjct: 458 WSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEI 517

Query: 483 HDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIV 542
           HDGPYN+IKNW+YT LV+A+HNG+GKCWT KV+ EEEL+ AIKTA  ++ D LCFIE IV
Sbjct: 518 HDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIV 577

Query: 543 HRDDTSKELLEFGNKIAAISSRPPKP 569
           H+DDTSKELLE+G++++A + RPP P
Sbjct: 578 HKDDTSKELLEWGSRVSAANGRPPNP 602

BLAST of Lag0012339 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 807.7 bits (2085), Expect = 5.7e-234
Identity = 387/563 (68.74%), Postives = 456/563 (80.99%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           +TLG YLARRL++IGV+D+FSVPGD NL LLD+ +AE  LKL+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGAC VTFTVGGLS++NAIAGAYSE+LP+ICIVGGPNSN YG+ +I+HHTIGLPDFT
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFT 164

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTC QA+I+NLE+A   IDTAIS  L+ESKPVYISIS NL AIP P+FS  P
Sbjct: 165 QELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRHP 224

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP  L  K +NQ+GL+ AVE  A+ LN AVKPV+VGG K+R AKA  AFVELADA GY 
Sbjct: 225 -VPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYG 284

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
           +AV PSAKG  PE+H HFIGTYWG VSTAFC E VE AD  +F G   ++  +V +SL  
Sbjct: 285 LAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK KAIIV+ DRV   NGP+FG +LMKDFL  LAKR+K N T+YENY RIY+PE  P++ 
Sbjct: 345 KKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD 404

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
            P E+LRVNVLF+HIQ MLS+  AV++ETGDSWF CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 405 NPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 464

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQ     RV+ CIGDGSFQ+  QDVSTM+R GQ  IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YTA V A+HNG+GKCWTAKV+ EEEL+ AI TA  ++ +  CFIE IVH+D
Sbjct: 525 PYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKD 584

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of Lag0012339 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 805.1 bits (2078), Expect = 3.7e-233
Identity = 385/563 (68.38%), Postives = 460/563 (81.71%), Query Frame = 0

Query: 6   STLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAADGYAR 65
           +TLG +LARRL+Q GV+D+FSVPGD NL LLD+ +AE  L L+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIGLPDFT 125
            RGVGAC VTFTVGGLS++NAIAGAYSE+LP+ICIVGGPNSN YG+ +I+HHTIGLPDF+
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 126 QELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPSFSIQP 185
           QELRCFQ VTC+QA+++NL+DA  +ID AIS  L+ESKPVYIS+S NL AIPH +FS  P
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP 224

Query: 186 LVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELADACGYA 245
            VP SL+P+ +N+MGLE AVE T + LN AVKPVMVGG KLR AKA  AFVELADA GYA
Sbjct: 225 -VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 246 VAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVAFSLTY 305
           +A+ PSAKG  PE+HPHFIGTYWG VST FC E VE AD  IF G   ++  +V +SL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPESGPVQS 365
           KK KAI+V+ DR+   NGP+FG ILM DF R L+KRVK N TAYENY RI++PE  P++ 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 366 EPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSSIGWSL 425
           E  E LRVN +F+HIQKMLS+  AVI+ETGDSWF CQKLKLP  CGYE Q+ Y SIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 426 GAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEVEIHDG 485
           GA LGYAQ + E+RV+  IGDGSFQ+  QD+STMLR GQ  IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEAIVHRD 545
           PYN+IKNW+YT LV+A+HNG+G CWTAKV+ EEEL+ AI TA  ++ DCLCFIE I+H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 546 DTSKELLEFGNKIAAISSRPPKP 569
           DTSKELLE+G++++A +SRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of Lag0012339 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 791.6 bits (2043), Expect = 4.3e-229
Identity = 381/568 (67.08%), Postives = 454/568 (79.93%), Query Frame = 0

Query: 1   MATTPSTLGHYLARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAA 60
           + ++ +TLG +L+RRL+Q GV+DIF+VPGD NL LLD  +A   L  +GCC+ELNAGYAA
Sbjct: 25  LGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAA 84

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVICIVGGPNSNYYGSKKIIHHTIG 120
           DGYAR RGVGAC VTFTVGGLS++NAIAGAYSE+LPVICIVGGPNSN +G+ +I+HHTIG
Sbjct: 85  DGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIG 144

Query: 121 LPDFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEESKPVYISISSNLVAIPHPS 180
           LPDF+QELRCFQ VTC+QA++++LEDA  +ID AI+  L ESKPVYISIS NL AIPHP+
Sbjct: 145 LPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCNLAAIPHPT 204

Query: 181 FSIQPLVPLSLSPKQTNQMGLEMAVEKTAQLLNNAVKPVMVGGKKLRPAKAQAAFVELAD 240
           F+  P VP  L+P+ +N+  LE AVE T + LN AVKPVMVGG KLR AKA+ AFVELAD
Sbjct: 205 FASYP-VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELAD 264

Query: 241 ACGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGATLDELETVA 300
           A GY VAV PSAKG  PENHPHFIGTYWG VST FC E VE AD  IF G   ++  +V 
Sbjct: 265 ASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVG 324

Query: 301 FSLTYKKNKAIIVELDRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAYENYGRIYIPES 360
           +SL  KK KAIIV  D VV  NGP+FG + M +F R LAKRVKPN TAYENY RI++PE 
Sbjct: 325 YSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAKRVKPNKTAYENYHRIFVPEG 384

Query: 361 GPVQSEPGEALRVNVLFKHIQKMLSNNMAVISETGDSWFQCQKLKLPDSCGYEIQLLYSS 420
            P++ +P E LR+N +F+HIQKMLSN  AVI+ETGDSWF CQKLKLP  CGYE Q+ Y S
Sbjct: 385 KPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGS 444

Query: 421 IGWSLGAALGYAQGASEERVVLCIGDGSFQMAPQDVSTMLRLGQNNIIFLINNGGYTIEV 480
           IGWS+GA LGYAQ   E+RV+  IGDGSFQ+  QDVSTM+R GQ  IIFLINNGGYTIEV
Sbjct: 445 IGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEV 504

Query: 481 EIHDGPYNIIKNWDYTALVNALHNGDGKCWTAKVQTEEELINAIKTAMEDQNDCLCFIEA 540
           EIHDGPYN+IKNW+YT LV+A+HNG+GKCWT KV+ EEEL+ AI TA  ++ D LCFIE 
Sbjct: 505 EIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTATLEKKDSLCFIEV 564

Query: 541 IVHRDDTSKELLEFGNKIAAISSRPPKP 569
           IVH+DDTSKELLE+G++++A + RPP P
Sbjct: 565 IVHKDDTSKELLEWGSRVSAANGRPPNP 591

BLAST of Lag0012339 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 79.7 bits (195), Expect = 8.3e-15
Identity = 118/502 (23.51%), Postives = 207/502 (41.24%), Query Frame = 0

Query: 3   TTPSTLGHYL-ARRLLQIGVSDIFSVPGDSNLVLLDYFVAEQGLKLVGCCSELNAGYAAD 62
           T PS  G+ L A+ L  +GV+ +F V G     L    +A  G++ +   +E +AGYAA 
Sbjct: 8   TPPSIDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMA-LGIRFIAFHNEQSAGYAAS 67

Query: 63  GYARRRGVGACAVTFTVGGLSLINAIAG---AYSEDLPVICIVGGPNSNYYGSKKIIHHT 122
            Y      G   +  TV G   ++ +AG   A+    P++ I G  +             
Sbjct: 68  AYGYL--TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCD----------QRD 127

Query: 123 IGLPDFTQELRCFQNVTCHQAIIDNLEDAQWKIDTAISKCLEES---KP--VYISISSNL 182
           +G  DF QEL   + V     + +  +D + +I   +S+ L+ +   +P   Y+ I +++
Sbjct: 128 VGRGDF-QELDQIEAVKAFSKLSEKAKDVR-EIPDCVSRVLDRAVSGRPGGCYLDIPTDV 187

Query: 183 VAIPHPSFSIQPLV-PLSLSPKQTNQMG-LEMAVEKTAQLLNNAVKPVMVGGKKLRPAKA 242
           +           LV  +  S K+    G L   +E    LL  A +P++V GK    ++A
Sbjct: 188 LRQKISESEADKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRA 247

Query: 243 QAAFVELADACGYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADGSIFVGA 302
           +    +L +  G     TP  KG+ P+ H  F  T   +++   C       D ++ VGA
Sbjct: 248 EDELKKLVEITGIPFLPTPMGKGLLPDTH-EFSATAARSLAIGKC-------DVALVVGA 307

Query: 303 TLDELETVAFSLTYKKN-KAIIVEL-DRVVFPNGPSFGPILMKDFLRALAKRVKPNTTAY 362
            L+ L     S  + K+ K I+V++ +  +    P  G +     +  L  R   +    
Sbjct: 308 RLNWLLHFGESPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPFC 367

Query: 363 ENYGRIYIPESGPVQSEPGEALRVN-----VLFKHIQKMLSNNMAVISETGDSWFQCQKL 422
                 ++        E GE + +      V F  +  M     A+++  G S     + 
Sbjct: 368 LGKSNSWVESISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEG 427

Query: 423 KLPDSCGYEIQL-------LYSSIGWSLGAALGY---AQGASEERVVLCI-GDGSFQMAP 476
                 G  + +       L +    ++G  LGY   A  AS +R+V+ + GD  F  + 
Sbjct: 428 ANTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSA 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144001.16.7e-29386.73pyruvate decarboxylase 1-like [Momordica charantia][more]
XP_038881352.11.3e-29186.55pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida][more]
TYK29657.17.7e-28984.83pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_008464213.12.9e-28884.66PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037741.1 pyruvate d... [more]
XP_023544035.11.4e-28584.93pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A2XFI32.2e-23868.92Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
Q10MW35.4e-23768.92Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 S... [more]
Q9FFT48.1e-23368.74Pyruvate decarboxylase 2 OS=Arabidopsis thaliana OX=3702 GN=PDC2 PE=2 SV=1[more]
Q9M0408.1e-23368.20Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1[more]
O826475.2e-23268.38Pyruvate decarboxylase 1 OS=Arabidopsis thaliana OX=3702 GN=PDC1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CS043.2e-29386.73Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1[more]
A0A5D3E0N53.7e-28984.83Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6... [more]
A0A5A7T8271.4e-28884.66Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3CKZ01.4e-28884.66Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502148 PE=3 SV=1[more]
A0A6J1IP685.5e-28584.22Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G01320.15.7e-23468.20Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G54960.15.7e-23468.74pyruvate decarboxylase-2 [more]
AT4G33070.13.7e-23368.38Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G01330.14.3e-22967.08pyruvate decarboxylase-3 [more]
AT5G17380.18.3e-1523.51Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 416..536
e-value: 1.4E-15
score: 57.4
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 1..563
e-value: 1.5E-191
score: 635.4
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 4..568
NoneNo IPR availableGENE3D3.40.50.970coord: 365..551
e-value: 5.4E-52
score: 178.0
NoneNo IPR availableGENE3D3.40.50.1220coord: 195..357
e-value: 3.4E-38
score: 132.8
NoneNo IPR availableGENE3D3.40.50.970coord: 3..180
e-value: 2.4E-57
score: 195.4
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 7..551
e-value: 1.8E-165
score: 549.1
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 4..568
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 11..171
e-value: 1.1076E-86
score: 263.971
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 370..552
e-value: 3.14403E-79
score: 245.522
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 205..316
e-value: 6.8E-21
score: 74.4
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 7..177
e-value: 3.8E-33
score: 114.5
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 372..560
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 7..182
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 200..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012339.1Lag0012339.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity