Lag0012204 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0012204
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5
Locationchr1: 38621306 .. 38624289 (+)
RNA-Seq ExpressionLag0012204
SyntenyLag0012204
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTAGCACCAAAGCTTCTCCATCCGCCCGCCGGCGTTCCCTACGCGCGTCCGTGCACTAGCCGCCGCTTCGTTCCTCATTGCCTCCATCGGCTCACCGAAAGCCGCCGCAGCAGAGGAAGAGGATTCCGACTTCCCGGAGTGGCTTCGGTCCCGTATCAACCGATCAATGTCGATTACTTGGAGGAGGAATTCAACGGCCACGGCCATGGAGTGACATTCGAAGGAATCGGCGATGAATGCGTCGCGAAATTGCGATTGGAAAATGGAGGTTCCGCGATTGTTATGTTCCCGAGCGGATTGATCACCTCCTACAAATCGCCGATGTGGCATGGAGGATCGCTGGAGCTGCTTCACTCTTCGGTTTCTGAGGACAAAAATGGCGATGCTATTGTTCGAGGAGGCGTATCCCTAGCCCTCGACTGTGGAAGCAATCGATTCGATGATCTTTCTTGGGCTCTTCATGATGTTAAAGGAAATCCTCGCGAGTCGATTCAGGTTTGGTTTAAATCTCATCTTAATGCGTTTTTAGCTTTTGAGTTTGTTTTATCTTGAGGTTCAGAATATCTATTTTAATTTTCTCACATTGAAGTGAAATAATGGTTAAATTTCAAGTTTGTTTTCTGAGGAACAAGTCTATCTAACAAGTTTTTATACTTTAAAAGGTTTCTACGTGTCCTAATTTGTGTTTTTTTTCAGTTCAATAACATGTGGGCGGGATGTTTGAACCCTAGACCTATTGTCGAGCATGTTCGATCGTGTTTATCTAATGATGTGAATTTTCAATTTTATATCTAGTAGGTCAATACCATTAACCCATTTTTTTAGTTTAACAAAATTTGAGAGTGGGAAGATTCGGATCTCTAAGATATGCTCATTTTGACAATAGCATTAACTCTATTAGCTTAATGCTTACATCACAAGCTTTCTAACATTGCTTGAAATTAAGTGTAATCTTAAATTCTTTCAAAAAAAAAAATGTAATCTTAAATAATAACATTTTTTTAACCATGATTAGTTACAAAATTAAAAGTTCAAGTTCAAGTATCTACTAAAAACTTTTTAAATTACAAGAACCAAATAAATACAAGATTGAAAGTTGAAAAACCTATCATAAACCTTTTTAAGTACACGGACTTGTTAGCTACAACCTAAAAAGTCCAGGACCAAACTTTGTAATTTAATTAGAAACAATTATTTGTCTTTTAACTTCATTTTGACTTCAACAAATGAGCAATGTGACTTCAAACATGAGTATTAATAAGTCATTTTCCAAATTCTAGAAACTAAAACAGCATAGAACAAATGGAGACTTAAACCATCTTTGATTGATTTGATATGAGCAGGTTGAGCTGATAAGCAGAGGTTCAGAGGGCATGGTCGAGCTGAAATACACAGTGACTCTTGGAGAAGACATGTTGACATCAGAGCTAAGAGTGTCCAACAATAAAGAAACGTCCCTTGAATTGAAGGGCAGTGTCTTAAGTCATTTGACTGTGAGCACACCAGAGGCAACCTATGCCATTGGATTGGAAGGGTCAGACTTCTACAGCAGCCCACCATTTTCTTCATCAAATGCTGCCATTGTCCCTCCAGATTATGGGCAGGGAAGCAGCAGCTCTGGGTTGAGTCAAACACTCAAAGAGATTCTTTCTGGTTGGGGTAAGAGCAGCCAAAGAGACAGTGAAGAGGAGATTGAAGGTGAAGAGACTGACAACTACAAGCAGTTGATGGAGCAAATGAGTAGGATCTACACCAGTGCTCCCAGGAGCTTCACAATCATAGACAGGGTAAGAAATGTTCTTCCAAAATTATGGTCTCATTTCGAGGAATTAGTTGTTCGTAGTGTTGTGTCGAAATTGTGATAGGAAAAAATGAAGAACCAAAACGATCACTTAAAAAAAACCAAAATAGACTTTTTGAAAGTATATAGAGTAAAATAAACTGAAGTTGAAAGTTTAGAGACTAAAATAAAGAAAATGTATGAATCAAAATGAATAGAAAGTAGAAGTATAGAGACTAAAGTATTATTTAAATATAAAAACTCAAACTTAAGGTCTCGTTGAAAGATCATTGGGTTTTTGGTTTCTGAAAATTAAGCTTATAAACACAATTTCCACTTATAAGTTTCTTTATTTTGTTACTTTCTTTCTTCTCATGTTTTCTAAAACCAAGTAAAGATTTGAAAACTAAAAAATAATAATTTTCAAAAATTTGTTTTTCTTTTTGAAATTTGGTTAAGAGTTAAGTAAATTGAAAGATAACAAGCATAATTTTCAAAAATCAGATAATTATCAAACGATTCTTTCCATTTTGGTTTTTGAATTTTTAAAATGTTCATTTTAATTCTTGTACTTACTAGTTTATTCCATTTATTCCAAAATGTCAATTTTCATCCCTTAATTTTTAAAAACTTATCCATTTTGATACTTATCTATATTTTGATTTTAACAAATAAATAACCAAATTCAAATACATTATTAACACGTTGGTGTAGTTGTATTGAAAATCTTGAGGGAACAAATATTTGATGACAAAATAATAGCATGGTCAACATGGCTATTTTTAAATACTTTTAAAAGTTCAAAGACTAAAATAGATATTTTTAAATTCAACTAAGTTGAATAAACACCAAAATGAAAATTCTTGAAAATTAAGAGACAAAAAGGAGATTTAAACCAACTTACAAACATTCTTCTAATTTGGTCCCAACCAGTTTCTCAGTTGGGTAGGAGCTTCAGTACCATGACTGATTTGAACCTTTTTTTTTTTTTGGCATGGAACAGGGAAGGAGAAACTCAGTGGTAGTGGGAAGAGATGGATTTGATGAACTTTACATATTCAGTCCTGGATCAAATCATGTATACTATGGAGAATATTCTTATATATGTGTTGGTCAGTCAGCCATGCTCAAACCAATAGTACTGGAGCCTCAACAAGTATGGACAGGCTCTCAGTATTTATACAACCCAAATCTCTAG

mRNA sequence

ATGAGCGTAGCACCAAAGCTTCTCCATCCGCCCGCCGGCGTTCCCTACGCGCGTCCGTGCACTAGCCGCCGCTTCGTTCCTCATTGCCTCCATCGGCTCACCGAAAGCCGCCGCAGCAGAGGAAGAGGATTCCGACTTCCCGGAGTGGCTTCGGTCCCGTATCAACCGATCAATGTCGATTACTTGGAGGAGGAATTCAACGGCCACGGCCATGGAGTGACATTCGAAGGAATCGGCGATGAATGCGTCGCGAAATTGCGATTGGAAAATGGAGGTTCCGCGATTGTTATGTTCCCGAGCGGATTGATCACCTCCTACAAATCGCCGATGTGGCATGGAGGATCGCTGGAGCTGCTTCACTCTTCGGTTTCTGAGGACAAAAATGGCGATGCTATTGTTCGAGGAGGCGTATCCCTAGCCCTCGACTGTGGAAGCAATCGATTCGATGATCTTTCTTGGGCTCTTCATGATGTTAAAGGAAATCCTCGCGAGTCGATTCAGGTTGAGCTGATAAGCAGAGGTTCAGAGGGCATGGTCGAGCTGAAATACACAGTGACTCTTGGAGAAGACATGTTGACATCAGAGCTAAGAGTGTCCAACAATAAAGAAACGTCCCTTGAATTGAAGGGCAGTGTCTTAAGTCATTTGACTGTGAGCACACCAGAGGCAACCTATGCCATTGGATTGGAAGGGTCAGACTTCTACAGCAGCCCACCATTTTCTTCATCAAATGCTGCCATTGTCCCTCCAGATTATGGGCAGGGAAGCAGCAGCTCTGGGTTGAGTCAAACACTCAAAGAGATTCTTTCTGGTTGGGGTAAGAGCAGCCAAAGAGACAGTGAAGAGGAGATTGAAGGTGAAGAGACTGACAACTACAAGCAGTTGATGGAGCAAATGAGTAGGATCTACACCAGTGCTCCCAGGAGCTTCACAATCATAGACAGGGGAAGGAGAAACTCAGTGGTAGTGGGAAGAGATGGATTTGATGAACTTTACATATTCAGTCCTGGATCAAATCATGTATACTATGGAGAATATTCTTATATATGTGTTGGTCAGTCAGCCATGCTCAAACCAATAGTACTGGAGCCTCAACAAGTATGGACAGGCTCTCAGTATTTATACAACCCAAATCTCTAG

Coding sequence (CDS)

ATGAGCGTAGCACCAAAGCTTCTCCATCCGCCCGCCGGCGTTCCCTACGCGCGTCCGTGCACTAGCCGCCGCTTCGTTCCTCATTGCCTCCATCGGCTCACCGAAAGCCGCCGCAGCAGAGGAAGAGGATTCCGACTTCCCGGAGTGGCTTCGGTCCCGTATCAACCGATCAATGTCGATTACTTGGAGGAGGAATTCAACGGCCACGGCCATGGAGTGACATTCGAAGGAATCGGCGATGAATGCGTCGCGAAATTGCGATTGGAAAATGGAGGTTCCGCGATTGTTATGTTCCCGAGCGGATTGATCACCTCCTACAAATCGCCGATGTGGCATGGAGGATCGCTGGAGCTGCTTCACTCTTCGGTTTCTGAGGACAAAAATGGCGATGCTATTGTTCGAGGAGGCGTATCCCTAGCCCTCGACTGTGGAAGCAATCGATTCGATGATCTTTCTTGGGCTCTTCATGATGTTAAAGGAAATCCTCGCGAGTCGATTCAGGTTGAGCTGATAAGCAGAGGTTCAGAGGGCATGGTCGAGCTGAAATACACAGTGACTCTTGGAGAAGACATGTTGACATCAGAGCTAAGAGTGTCCAACAATAAAGAAACGTCCCTTGAATTGAAGGGCAGTGTCTTAAGTCATTTGACTGTGAGCACACCAGAGGCAACCTATGCCATTGGATTGGAAGGGTCAGACTTCTACAGCAGCCCACCATTTTCTTCATCAAATGCTGCCATTGTCCCTCCAGATTATGGGCAGGGAAGCAGCAGCTCTGGGTTGAGTCAAACACTCAAAGAGATTCTTTCTGGTTGGGGTAAGAGCAGCCAAAGAGACAGTGAAGAGGAGATTGAAGGTGAAGAGACTGACAACTACAAGCAGTTGATGGAGCAAATGAGTAGGATCTACACCAGTGCTCCCAGGAGCTTCACAATCATAGACAGGGGAAGGAGAAACTCAGTGGTAGTGGGAAGAGATGGATTTGATGAACTTTACATATTCAGTCCTGGATCAAATCATGTATACTATGGAGAATATTCTTATATATGTGTTGGTCAGTCAGCCATGCTCAAACCAATAGTACTGGAGCCTCAACAAGTATGGACAGGCTCTCAGTATTTATACAACCCAAATCTCTAG

Protein sequence

MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLHSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKPIVLEPQQVWTGSQYLYNPNL
Homology
BLAST of Lag0012204 vs. NCBI nr
Match: XP_038883421.1 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Benincasa hispida])

HSP 1 Score: 664.8 bits (1714), Expect = 4.3e-187
Identity = 332/380 (87.37%), Postives = 354/380 (93.16%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCT-SRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINV 60
           MSVAPKLL+PP G+PYARP T  RRFV HCLH+ T SRRS GRGFRLPGVASVPYQPINV
Sbjct: 1   MSVAPKLLYPPTGIPYARPWTLRRRFVCHCLHQSTGSRRSGGRGFRLPGVASVPYQPINV 60

Query: 61  DYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELL 120
           DYLEEEFNG GHGVTFEGIGDECVAKLRLENGGSAI+MFPSGLITSYKSPMWHGGSLELL
Sbjct: 61  DYLEEEFNGQGHGVTFEGIGDECVAKLRLENGGSAILMFPSGLITSYKSPMWHGGSLELL 120

Query: 121 HSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMV 180
           HSSVSEDKNGD +V+GGV+LALDCGSNR +D+SWALH+V GNPRESIQ+ELISR +EG V
Sbjct: 121 HSSVSEDKNGDVVVQGGVALALDCGSNRLEDVSWALHNVTGNPRESIQMELISRVAEGKV 180

Query: 181 ELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPP 240
           ELKYTVTLGEDMLTSEL VSNNKETSLELKG VLSH+TVSTPEAT+AIGLEGSDF+S PP
Sbjct: 181 ELKYTVTLGEDMLTSELTVSNNKETSLELKGCVLSHMTVSTPEATFAIGLEGSDFHSMPP 240

Query: 241 FSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQM 300
           FSSS+A I P  YGQG SSSGL+QT + ILSGWGKS+QRDSEEEIEGEETDNYKQLMEQM
Sbjct: 241 FSSSSAIIPPDCYGQG-SSSGLTQTFEGILSGWGKSNQRDSEEEIEGEETDNYKQLMEQM 300

Query: 301 SRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKP 360
           SRIYT+APR FTIIDRGRRNSVVVGRDGFDE+YIFSPGSNHVYYGEYSYIC+GQSAMLKP
Sbjct: 301 SRIYTNAPRYFTIIDRGRRNSVVVGRDGFDEVYIFSPGSNHVYYGEYSYICLGQSAMLKP 360

Query: 361 IVLEPQQVWTGSQYLYNPNL 380
           IVLEPQQVW GSQYLYNPNL
Sbjct: 361 IVLEPQQVWRGSQYLYNPNL 379

BLAST of Lag0012204 vs. NCBI nr
Match: KAG6595507.1 (Protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.6e-173
Identity = 314/382 (82.20%), Postives = 339/382 (88.74%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLL+P AGV Y RP T RRFV  CL+R T SRRSRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLYPAAGVLYTRPSTRRRFVYQCLNRSTGSRRSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGLGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD +VRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELISR +EG VE
Sbjct: 121 SSVCEDENGDVVVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISRAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDML SEL VSNN++T+++LKGSVLSHLTVSTPEATYAIGLEGSDFYS  P 
Sbjct: 181 LKYIVTLGEDMLRSELIVSNNEQTAIQLKGSVLSHLTVSTPEATYAIGLEGSDFYSRAPC 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEI---EGEETDNYKQLME 300
           SSS  +IVPPDYGQG S SGL +TL  ILSGWGKS +++SEEE+   EGEETDNYKQL E
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLGRTLNGILSGWGKSKEKESEEEVEGEEGEETDNYKQLTE 300

Query: 301 QMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAML 360
           +MSRIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAML
Sbjct: 301 EMSRIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAML 360

Query: 361 KPIVLEPQQVWTGSQYLYNPNL 380
           KPI+L+P QVW GSQ L+NPNL
Sbjct: 361 KPILLQPHQVWRGSQSLFNPNL 380

BLAST of Lag0012204 vs. NCBI nr
Match: XP_023517875.1 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.7 bits (1589), Expect = 1.3e-172
Identity = 313/382 (81.94%), Postives = 338/382 (88.48%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLL+P AGV Y RP T RRFV  CL+R T SRRSRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLYPAAGVLYKRPSTRRRFVYQCLNRSTGSRRSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGLGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD +VRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELIS  +EG VE
Sbjct: 121 SSVCEDENGDVVVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISGAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDML SEL VSNN++T+++LKGSVLSHLTVSTPEATYAIGLEGSDFYS  P 
Sbjct: 181 LKYIVTLGEDMLRSELIVSNNEQTAIQLKGSVLSHLTVSTPEATYAIGLEGSDFYSRAPC 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEI---EGEETDNYKQLME 300
           SSS  +IVPPDYGQG S SGL +TL  ILSGWGKS +++SEEE+   EGEETDNYKQL E
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLGRTLNGILSGWGKSKEKESEEEVEGEEGEETDNYKQLTE 300

Query: 301 QMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAML 360
           +MSRIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAML
Sbjct: 301 EMSRIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAML 360

Query: 361 KPIVLEPQQVWTGSQYLYNPNL 380
           KPI+L+P QVW GSQ L+NPNL
Sbjct: 361 KPILLQPHQVWRGSQSLFNPNL 380

BLAST of Lag0012204 vs. NCBI nr
Match: XP_022966365.1 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita maxima])

HSP 1 Score: 614.8 bits (1584), Expect = 5.1e-172
Identity = 314/382 (82.20%), Postives = 336/382 (87.96%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLLHP AGV Y RP T RRFV  CL+  T SR SRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLHPAAGVLYKRPFTRRRFVYQCLNPSTGSRGSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGIGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD IVRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELISR +EG VE
Sbjct: 121 SSVCEDENGDVIVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISRAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDMLTSEL VSNN+E++++LKGSVLSHLTVSTPEATYAIGLEGSDFYS  PF
Sbjct: 181 LKYIVTLGEDMLTSELVVSNNEESAIQLKGSVLSHLTVSTPEATYAIGLEGSDFYSRAPF 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEI---EGEETDNYKQLME 300
           SSS  +IVPPDYGQG S SGL +T   IL GWGKS +++SEEE+   EGEETDNYKQL E
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLRRTWNGILPGWGKSKEKESEEEVEGEEGEETDNYKQLTE 300

Query: 301 QMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAML 360
           +MSRIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAML
Sbjct: 301 EMSRIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAML 360

Query: 361 KPIVLEPQQVWTGSQYLYNPNL 380
           K I+LEP QVW GSQ L+NPNL
Sbjct: 361 KSILLEPHQVWRGSQSLFNPNL 380

BLAST of Lag0012204 vs. NCBI nr
Match: XP_022925215.1 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 5.1e-172
Identity = 312/379 (82.32%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLLHP AGV Y RP T RRFV  CL+R T SR SRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLHPAAGVLYKRPSTRRRFVYQCLNRSTGSRSSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGLGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD +VRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELISR +EG VE
Sbjct: 121 SSVCEDENGDVVVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISRAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDMLTSEL VSNN++T+++LKGSVLSHLT STPEATYAIGLEGSDFYS+ PF
Sbjct: 181 LKYIVTLGEDMLTSELIVSNNEQTAIQLKGSVLSHLTASTPEATYAIGLEGSDFYSTAPF 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQMS 300
           SSS  +IVPPDYGQG S SGL +TL  ILSGWGKS  ++  E  EGEETDNYKQL E+MS
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLGRTLNGILSGWGKS--KEEVEGEEGEETDNYKQLTEEMS 300

Query: 301 RIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKPI 360
           RIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAMLKPI
Sbjct: 301 RIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAMLKPI 360

Query: 361 VLEPQQVWTGSQYLYNPNL 380
           +L+P QVW GSQ L+NPNL
Sbjct: 361 LLQPHQVWRGSQSLFNPNL 375

BLAST of Lag0012204 vs. ExPASy Swiss-Prot
Match: Q9C503 (Protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Arabidopsis thaliana OX=3702 GN=NDF5 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.5e-70
Identity = 149/329 (45.29%), Postives = 215/329 (65.35%), Query Frame = 0

Query: 53  PYQPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWH 112
           P  PI+V+YL  EF+  GHG TFE IG+ C+A+L+L+NG SA VM   G+ITSYK  +WH
Sbjct: 56  PAFPIDVEYLRREFS--GHGATFEDIGETCIARLKLDNGSSANVMLTRGMITSYKVRVWH 115

Query: 113 GGSLELLHSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLS-WALHDVKGNPRESIQVELI 172
           GG +ELLH+ V +++  + ++RGGVS A    S+  D++S W L  + G+ ++ +Q+EL 
Sbjct: 116 GGKVELLHTWVEQEEE-EVVIRGGVSSAF--RSSDSDEISDWRLQGISGDSKDCVQMELR 175

Query: 173 SRGSE-GMVELKYTVTLGEDMLTSELRVSNNKETSLELKG-SVLSHLTVSTPEATYAIGL 232
               +   +ELK  ++L E+ L+ EL ++N   + ++L+G S++S+LTVSTPEATYA+GL
Sbjct: 176 RSDKKIKEIELKQIISLRENTLSIELSMTNKGISPIKLEGCSLVSYLTVSTPEATYAVGL 235

Query: 233 EGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEET 292
           EGSDF  + PF         P +G              ++ G     + + +    GEE 
Sbjct: 236 EGSDFVETTPFL--------PRFG--------------VVQG----EKEEEKPGFGGEEE 295

Query: 293 DNYKQLMEQMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYI 352
            NYKQL  +MSRIYT AP+SFT+IDRGRRNSVVVGR+GF+E+Y++SPGS    Y + +Y+
Sbjct: 296 SNYKQLNREMSRIYTCAPKSFTVIDRGRRNSVVVGREGFEEVYMYSPGSRLESYTKSAYV 353

Query: 353 CVGQSAMLKPIVLEPQQVWTGSQYLYNPN 379
           C+G S++L PI LE   VW G  +L+NPN
Sbjct: 356 CIGPSSLLSPISLESGCVWRGVLHLHNPN 353

BLAST of Lag0012204 vs. ExPASy Swiss-Prot
Match: Q94AQ8 (Photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PNSB2 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 2.0e-22
Identity = 91/345 (26.38%), Postives = 155/345 (44.93%), Query Frame = 0

Query: 55  QPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPM-WHG 114
           Q IN + LE++F     G+ F    +  + +L++ NG S  +      + SYK  + W  
Sbjct: 26  QTINTEKLEDKFG--RKGIKFSESNNIPMVELKVRNGSSLKLSLSDAHVLSYKPKVYWKD 85

Query: 115 GSLELLHSSVSEDKNGDAIVRGGVSLAL------DCGSNRFDDLSWALHDVKGNPRESIQ 174
              E +  +V  D++     RGGV + +        GS+      W++ D   +  +++Q
Sbjct: 86  EGFEEVLYTVDGDES-----RGGVGVVIVNGEEPKGGSSVISGCDWSVKDTDSDAIDALQ 145

Query: 175 VELISRGSEGMVELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAI 234
           +EL    + G++++ Y V+L    + + L V NN    + LK  ++S+L           
Sbjct: 146 IELSC--TAGVLDITYIVSLYPVSMATALVVKNNGRKPVTLKPGIMSYLRFKKRSGAGIQ 205

Query: 235 GLEGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGE 294
           GL+G  +  +PP SS    + P            S+ +K   SGW   S+   +  I   
Sbjct: 206 GLKGCSYCPNPPLSSPFELLSP------------SEAMKAESSGW-FGSEEGEKPGIWAV 265

Query: 295 ETDNYKQLMEQMSRI------------YTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFS 354
           E      L ++MSRI            Y + P  F  ID+GR     + R GF+E+Y+ S
Sbjct: 266 EDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQGRGLFFRMIRIGFEEMYVGS 325

Query: 355 PGSNHVYYGEYSY-ICVGQSAMLKPIVLEPQQVWTGSQYLYNPNL 380
           PGS    YG+  Y +C G ++ML P+ +   + W G+  + + NL
Sbjct: 326 PGSMWDKYGKQHYFVCTGPTSMLVPVDVASGETWRGAMVIEHDNL 348

BLAST of Lag0012204 vs. ExPASy TrEMBL
Match: A0A6J1HRF2 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucurbita maxima OX=3661 GN=LOC111466037 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.5e-172
Identity = 314/382 (82.20%), Postives = 336/382 (87.96%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLLHP AGV Y RP T RRFV  CL+  T SR SRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLHPAAGVLYKRPFTRRRFVYQCLNPSTGSRGSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGIGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD IVRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELISR +EG VE
Sbjct: 121 SSVCEDENGDVIVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISRAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDMLTSEL VSNN+E++++LKGSVLSHLTVSTPEATYAIGLEGSDFYS  PF
Sbjct: 181 LKYIVTLGEDMLTSELVVSNNEESAIQLKGSVLSHLTVSTPEATYAIGLEGSDFYSRAPF 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEI---EGEETDNYKQLME 300
           SSS  +IVPPDYGQG S SGL +T   IL GWGKS +++SEEE+   EGEETDNYKQL E
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLRRTWNGILPGWGKSKEKESEEEVEGEEGEETDNYKQLTE 300

Query: 301 QMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAML 360
           +MSRIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAML
Sbjct: 301 EMSRIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAML 360

Query: 361 KPIVLEPQQVWTGSQYLYNPNL 380
           K I+LEP QVW GSQ L+NPNL
Sbjct: 361 KSILLEPHQVWRGSQSLFNPNL 380

BLAST of Lag0012204 vs. ExPASy TrEMBL
Match: A0A6J1EHB0 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucurbita moschata OX=3662 GN=LOC111432524 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.5e-172
Identity = 312/379 (82.32%), Postives = 335/379 (88.39%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MSVAPKLLHP AGV Y RP T RRFV  CL+R T SR SRGRGF+LP VASVPYQPINVD
Sbjct: 1   MSVAPKLLHPAAGVLYKRPSTRRRFVYQCLNRSTGSRSSRGRGFQLPRVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGV FEGIGDEC AKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH
Sbjct: 61  YLEEEFNGLGHGVRFEGIGDECAAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSV ED+NGD +VRGGVSLALDCGS R DD+ W LHDVKGNPRESIQVELISR +EG VE
Sbjct: 121 SSVCEDENGDVVVRGGVSLALDCGSIRLDDVCWTLHDVKGNPRESIQVELISRAAEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKY VTLGEDMLTSEL VSNN++T+++LKGSVLSHLT STPEATYAIGLEGSDFYS+ PF
Sbjct: 181 LKYIVTLGEDMLTSELIVSNNEQTAIQLKGSVLSHLTASTPEATYAIGLEGSDFYSTAPF 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQMS 300
           SSS  +IVPPDYGQG S SGL +TL  ILSGWGKS  ++  E  EGEETDNYKQL E+MS
Sbjct: 241 SSS-YSIVPPDYGQG-SGSGLGRTLNGILSGWGKS--KEEVEGEEGEETDNYKQLTEEMS 300

Query: 301 RIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKPI 360
           RIYTSAPR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEYS+ICVG SAMLKPI
Sbjct: 301 RIYTSAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEYSFICVGHSAMLKPI 360

Query: 361 VLEPQQVWTGSQYLYNPNL 380
           +L+P QVW GSQ L+NPNL
Sbjct: 361 LLQPHQVWRGSQSLFNPNL 375

BLAST of Lag0012204 vs. ExPASy TrEMBL
Match: A0A1S3B450 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucumis melo OX=3656 GN=LOC103485977 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.8e-168
Identity = 298/379 (78.63%), Postives = 339/379 (89.45%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MS+A KLLHPP  +PY  P   R F+ H LHR T +R   GR F LPGVASVPYQPINVD
Sbjct: 1   MSIASKLLHPPPTIPYTPP---RPFLSHSLHRPTATR--LGRAFPLPGVASVPYQPINVD 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLE+EFNG GHGVTFEGIGDEC+AKLRLENGGSAI+MFPSGLITSYKSPMWHGGS+ELLH
Sbjct: 61  YLEDEFNGQGHGVTFEGIGDECLAKLRLENGGSAILMFPSGLITSYKSPMWHGGSVELLH 120

Query: 121 SSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMVE 180
           SSVSEDKNGD +V+GGVS+ALDCGS+R +++SW LH+VKGNPRESIQVELISRG EG VE
Sbjct: 121 SSVSEDKNGDVVVQGGVSVALDCGSHRLENVSWDLHNVKGNPRESIQVELISRGLEGKVE 180

Query: 181 LKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPPF 240
           LKYTVTLGED+LTSEL V NNK+TSLELKG +LSHLT+STPEAT+AIGLEGS+F+S PPF
Sbjct: 181 LKYTVTLGEDVLTSELTVCNNKQTSLELKGYILSHLTLSTPEATFAIGLEGSNFHSIPPF 240

Query: 241 SSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQMS 300
            SSN AI+PPD      SSGL+Q L+ I+SGWGKSSQR+S EEIEGEE DNYKQL++++S
Sbjct: 241 -SSNHAIIPPD-----CSSGLTQRLQGIVSGWGKSSQRESGEEIEGEEMDNYKQLVDKIS 300

Query: 301 RIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKPI 360
           RIYTSAPR+FTIIDRGRRNSVV+GRDGFDE+Y+FSPGSNHVYYGEYSY+C+GQSA+LKPI
Sbjct: 301 RIYTSAPRNFTIIDRGRRNSVVIGRDGFDEVYMFSPGSNHVYYGEYSYVCIGQSAILKPI 360

Query: 361 VLEPQQVWTGSQYLYNPNL 380
           +LEPQQ+WTGSQYLYNPNL
Sbjct: 361 LLEPQQLWTGSQYLYNPNL 368

BLAST of Lag0012204 vs. ExPASy TrEMBL
Match: A0A0A0KWN8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192150 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.8e-165
Identity = 293/380 (77.11%), Postives = 332/380 (87.37%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTS-RRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINV 60
           MS+  KLL PP  +PY R  TS R F  HCLH  T S R  GR F LPGVAS+PYQPINV
Sbjct: 1   MSLPSKLLQPPPTIPYTRLSTSPRPFFCHCLHPSTPSSRGGGRAFPLPGVASLPYQPINV 60

Query: 61  DYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELL 120
           DYLEEEFNG GHGVTFEGIGDEC+AKLRLENGGSAI+MFPSGLITSYKSPMWHGGSLELL
Sbjct: 61  DYLEEEFNGQGHGVTFEGIGDECLAKLRLENGGSAILMFPSGLITSYKSPMWHGGSLELL 120

Query: 121 HSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSEGMV 180
           HSSVSEDKNGD +V+GGVS+ALD G++R +D+SW LH+V+GNPRESIQ+ELISR  EGMV
Sbjct: 121 HSSVSEDKNGDVVVQGGVSVALDYGTHRLEDVSWDLHNVEGNPRESIQIELISRALEGMV 180

Query: 181 ELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYSSPP 240
           ELKYTVTLGED+LTSEL V NN +TSLELKG +LSH+T+STPEAT+AIGLEGS+F+S PP
Sbjct: 181 ELKYTVTLGEDVLTSELTVCNNNQTSLELKGYILSHMTLSTPEATFAIGLEGSNFHSVPP 240

Query: 241 FSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEETDNYKQLMEQM 300
           F SSN AI+PPD      SSGL+Q LK I+SGWGKS +R+S EEIEGEE DNYKQLM+QM
Sbjct: 241 F-SSNHAIIPPD-----CSSGLTQRLKGIVSGWGKSRERESGEEIEGEEMDNYKQLMDQM 300

Query: 301 SRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYICVGQSAMLKP 360
           SRIYT+APR+FTIIDRGRRNSVVVGR+GFDE+Y+ SPGSNHVYYGEYSY+C+G SAMLKP
Sbjct: 301 SRIYTNAPRNFTIIDRGRRNSVVVGREGFDEVYMLSPGSNHVYYGEYSYVCIGHSAMLKP 360

Query: 361 IVLEPQQVWTGSQYLYNPNL 380
           I+L+PQQ+W GSQYLYNPNL
Sbjct: 361 ILLQPQQLWRGSQYLYNPNL 374

BLAST of Lag0012204 vs. ExPASy TrEMBL
Match: A0A6J1DST2 (protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Momordica charantia OX=3673 GN=LOC111023162 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.5e-158
Identity = 301/393 (76.59%), Postives = 329/393 (83.72%), Query Frame = 0

Query: 1   MSVAPKLLHPPAGVPYARPCTSRRFVPHCLHRLTESRRSRGRGFRLPGVASVPYQPINVD 60
           MS   + LHPPA VPYARP T RR       R TE  RSR R FRLP VASVPY+P+NV+
Sbjct: 1   MSTGARFLHPPAVVPYARPSTRRRIAHPRFLRSTEI-RSRVRRFRLPEVASVPYEPMNVE 60

Query: 61  YLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWHGGSLELLH 120
           YLEEEFNG GHGVTFEGIGDE VAKLRLENGGSAI+M PSGLITSYKSPMWHGGS+E+LH
Sbjct: 61  YLEEEFNGQGHGVTFEGIGDELVAKLRLENGGSAILMLPSGLITSYKSPMWHGGSIEILH 120

Query: 121 SSV--SEDKNGDAIVRGGVS--LALDCGSNRFDDLSWALHDVKGNPRESIQVELISRGSE 180
           SSV  SE+  G  +VRGGVS  LALDCG NRFD +SWALHDV+GNP +SIQVEL SR SE
Sbjct: 121 SSVSESEENGGGGVVRGGVSVALALDCGDNRFDAVSWALHDVRGNPSDSIQVELRSRVSE 180

Query: 181 GMVELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAIGLEGSDFYS 240
           GMVELKY VTL ED+LTSEL VSNN+ETSL+LKG +LSHLTVSTPEATYAIGLEGS+F  
Sbjct: 181 GMVELKYLVTLEEDLLTSELTVSNNRETSLDLKGCILSHLTVSTPEATYAIGLEGSEFQR 240

Query: 241 SP-PFSSSNAAIVPPDYGQGSSSSG------LSQTLKEILSGWG--KSSQRDSE-EEIEG 300
            P P  SSN+AIVPPDYGQGS S        L QTLK IL GWG  +SSQR+SE EE  G
Sbjct: 241 RPWPPLSSNSAIVPPDYGQGSGSGSGNANGLLGQTLKGILPGWGRSRSSQRESEDEESLG 300

Query: 301 EETDNYKQLMEQMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEY 360
           EETDNYKQLMEQMSRIYT+APR+FTIIDRGRRNSVVVGRDGFDELY+FSPGSNHVYYGEY
Sbjct: 301 EETDNYKQLMEQMSRIYTNAPRNFTIIDRGRRNSVVVGRDGFDELYMFSPGSNHVYYGEY 360

Query: 361 SYICVGQSAMLKPIVLEPQQVWTGSQYLYNPNL 380
           SYICV QSA+LKPI+L+PQQVWTGSQ+L+NPNL
Sbjct: 361 SYICVAQSAILKPILLQPQQVWTGSQHLHNPNL 392

BLAST of Lag0012204 vs. TAIR 10
Match: AT1G55370.2 (NDH-dependent cyclic electron flow 5 )

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-71
Identity = 149/329 (45.29%), Postives = 215/329 (65.35%), Query Frame = 0

Query: 53  PYQPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWH 112
           P  PI+V+YL  EF+  GHG TFE IG+ C+A+L+L+NG SA VM   G+ITSYK  +WH
Sbjct: 56  PAFPIDVEYLRREFS--GHGATFEDIGETCIARLKLDNGSSANVMLTRGMITSYKVRVWH 115

Query: 113 GGSLELLHSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLS-WALHDVKGNPRESIQVELI 172
           GG +ELLH+ V +++  + ++RGGVS A    S+  D++S W L  + G+ ++ +Q+EL 
Sbjct: 116 GGKVELLHTWVEQEEE-EVVIRGGVSSAF--RSSDSDEISDWRLQGISGDSKDCVQMELR 175

Query: 173 SRGSE-GMVELKYTVTLGEDMLTSELRVSNNKETSLELKG-SVLSHLTVSTPEATYAIGL 232
               +   +ELK  ++L E+ L+ EL ++N   + ++L+G S++S+LTVSTPEATYA+GL
Sbjct: 176 RSDKKIKEIELKQIISLRENTLSIELSMTNKGISPIKLEGCSLVSYLTVSTPEATYAVGL 235

Query: 233 EGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEET 292
           EGSDF  + PF         P +G              ++ G     + + +    GEE 
Sbjct: 236 EGSDFVETTPFL--------PRFG--------------VVQG----EKEEEKPGFGGEEE 295

Query: 293 DNYKQLMEQMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVYYGEYSYI 352
            NYKQL  +MSRIYT AP+SFT+IDRGRRNSVVVGR+GF+E+Y++SPGS    Y + +Y+
Sbjct: 296 SNYKQLNREMSRIYTCAPKSFTVIDRGRRNSVVVGREGFEEVYMYSPGSRLESYTKSAYV 353

Query: 353 CVGQSAMLKPIVLEPQQVWTGSQYLYNPN 379
           C+G S++L PI LE   VW G  +L+NPN
Sbjct: 356 CIGPSSLLSPISLESGCVWRGVLHLHNPN 353

BLAST of Lag0012204 vs. TAIR 10
Match: AT1G55370.1 (NDH-dependent cyclic electron flow 5 )

HSP 1 Score: 192.2 bits (487), Expect = 7.6e-49
Identity = 114/266 (42.86%), Postives = 167/266 (62.78%), Query Frame = 0

Query: 53  PYQPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPMWH 112
           P  PI+V+YL  EF+  GHG TFE IG+ C+A+L+L+NG SA VM   G+ITSYK  +WH
Sbjct: 56  PAFPIDVEYLRREFS--GHGATFEDIGETCIARLKLDNGSSANVMLTRGMITSYKVRVWH 115

Query: 113 GGSLELLHSSVSEDKNGDAIVRGGVSLALDCGSNRFDDLS-WALHDVKGNPRESIQVELI 172
           GG +ELLH+ V +++  + ++RGGVS A    S+  D++S W L  + G+ ++ +Q+EL 
Sbjct: 116 GGKVELLHTWVEQEEE-EVVIRGGVSSAF--RSSDSDEISDWRLQGISGDSKDCVQMELR 175

Query: 173 SRGSE-GMVELKYTVTLGEDMLTSELRVSNNKETSLELKG-SVLSHLTVSTPEATYAIGL 232
               +   +ELK  ++L E+ L+ EL ++N   + ++L+G S++S+LTVSTPEATYA+GL
Sbjct: 176 RSDKKIKEIELKQIISLRENTLSIELSMTNKGISPIKLEGCSLVSYLTVSTPEATYAVGL 235

Query: 233 EGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGEET 292
           EGSDF  + PF         P +G              ++ G     + + +    GEE 
Sbjct: 236 EGSDFVETTPFL--------PRFG--------------VVQG----EKEEEKPGFGGEEE 290

Query: 293 DNYKQLMEQMSRIYTSAPRSFTIIDR 316
            NYKQL  +MSRIYT AP+SFT+IDR
Sbjct: 296 SNYKQLNREMSRIYTCAPKSFTVIDR 290

BLAST of Lag0012204 vs. TAIR 10
Match: AT1G64770.1 (NDH-dependent cyclic electron flow 1 )

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-23
Identity = 91/345 (26.38%), Postives = 155/345 (44.93%), Query Frame = 0

Query: 55  QPINVDYLEEEFNGHGHGVTFEGIGDECVAKLRLENGGSAIVMFPSGLITSYKSPM-WHG 114
           Q IN + LE++F     G+ F    +  + +L++ NG S  +      + SYK  + W  
Sbjct: 26  QTINTEKLEDKFG--RKGIKFSESNNIPMVELKVRNGSSLKLSLSDAHVLSYKPKVYWKD 85

Query: 115 GSLELLHSSVSEDKNGDAIVRGGVSLAL------DCGSNRFDDLSWALHDVKGNPRESIQ 174
              E +  +V  D++     RGGV + +        GS+      W++ D   +  +++Q
Sbjct: 86  EGFEEVLYTVDGDES-----RGGVGVVIVNGEEPKGGSSVISGCDWSVKDTDSDAIDALQ 145

Query: 175 VELISRGSEGMVELKYTVTLGEDMLTSELRVSNNKETSLELKGSVLSHLTVSTPEATYAI 234
           +EL    + G++++ Y V+L    + + L V NN    + LK  ++S+L           
Sbjct: 146 IELSC--TAGVLDITYIVSLYPVSMATALVVKNNGRKPVTLKPGIMSYLRFKKRSGAGIQ 205

Query: 235 GLEGSDFYSSPPFSSSNAAIVPPDYGQGSSSSGLSQTLKEILSGWGKSSQRDSEEEIEGE 294
           GL+G  +  +PP SS    + P            S+ +K   SGW   S+   +  I   
Sbjct: 206 GLKGCSYCPNPPLSSPFELLSP------------SEAMKAESSGW-FGSEEGEKPGIWAV 265

Query: 295 ETDNYKQLMEQMSRI------------YTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFS 354
           E      L ++MSRI            Y + P  F  ID+GR     + R GF+E+Y+ S
Sbjct: 266 EDSVITLLEKKMSRIYGAPPAERLKAVYNTPPSKFETIDQGRGLFFRMIRIGFEEMYVGS 325

Query: 355 PGSNHVYYGEYSY-ICVGQSAMLKPIVLEPQQVWTGSQYLYNPNL 380
           PGS    YG+  Y +C G ++ML P+ +   + W G+  + + NL
Sbjct: 326 PGSMWDKYGKQHYFVCTGPTSMLVPVDVASGETWRGAMVIEHDNL 348

BLAST of Lag0012204 vs. TAIR 10
Match: AT4G25900.1 (Galactose mutarotase-like superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 7.5e-04
Identity = 21/77 (27.27%), Postives = 41/77 (53.25%), Query Frame = 0

Query: 298 QMSRIYTSAPRSFTIIDRGRRNSVVVGRDGFDELYIFSPGSNHVY---YGEYS-YICVGQ 357
           Q+ R+Y S P    I+D  ++ ++VV ++G  +  +++P    V      +Y  ++ V  
Sbjct: 229 QLDRLYLSTPDQLRIVDHKKKKTIVVHKEGQVDAVVWNPWDKKVSDLGVEDYKRFVTVES 288

Query: 358 SAMLKPIVLEPQQVWTG 371
           +A+ KPI + P + W G
Sbjct: 289 AAVAKPITVNPGKEWKG 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883421.14.3e-18787.37protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Benincasa hispida][more]
KAG6595507.11.6e-17382.20Protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5, partial [Cucurbita argyrosperma su... [more]
XP_023517875.11.3e-17281.94protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita pepo subsp. pepo][more]
XP_022966365.15.1e-17282.20protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita maxima][more]
XP_022925215.15.1e-17282.32protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C5031.5e-7045.29Protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q94AQ82.0e-2226.38Photosynthetic NDH subunit of subcomplex B 2, chloroplastic OS=Arabidopsis thali... [more]
Match NameE-valueIdentityDescription
A0A6J1HRF22.5e-17282.20protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1EHB02.5e-17282.32protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3B4504.8e-16878.63protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KWN83.8e-16577.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192150 PE=4 SV=1[more]
A0A6J1DST23.5e-15876.59protein NDH-DEPENDENT CYCLIC ELECTRON FLOW 5 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G55370.21.1e-7145.29NDH-dependent cyclic electron flow 5 [more]
AT1G55370.17.6e-4942.86NDH-dependent cyclic electron flow 5 [more]
AT1G64770.11.4e-2326.38NDH-dependent cyclic electron flow 1 [more]
AT4G25900.17.5e-0427.27Galactose mutarotase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 79..377
e-value: 8.4E-23
score: 82.9
NoneNo IPR availablePANTHERPTHR11122APOSPORY-ASSOCIATED PROTEIN C-RELATEDcoord: 38..379
NoneNo IPR availablePANTHERPTHR11122:SF15PROTEIN NDH-DEPENDENT CYCLIC ELECTRON FLOW 5coord: 38..379
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 81..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0012204.1Lag0012204.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0047938 glucose-6-phosphate 1-epimerase activity
molecular_function GO:0003824 catalytic activity