Lag0011762 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011762
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPeptidase A1 domain-containing protein
Locationchr1: 32442859 .. 32443311 (-)
RNA-Seq ExpressionLag0011762
SyntenyLag0011762
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGTTCCCTCATCACAATCCCCCTCACCACCTTCCCTTCAATTCCAGTTACAGATCTATGGCAGAACATTAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAATCCAAACAGTTCCATCGAGAAAGTCTCTATGTTCTCTCGTAGCTATGTAGCTTATTCAATCTCCCTCAGCTTTGGAACTCCATCGCAGAATTTGTTGTTCGTGTTCGATACTGGAAGTAGCCTCGTCTGGTTCCCCTACACTGTCGGTTGTCTCTGTTCCAATTCTTCGTTTCCGAACTCAGACGATTGCGAAGTTTATCCCCAAATTATCTTCCTCTGTGAAGATCGTCGGTTGCTGAAATCGACGGAAAGGTGA

mRNA sequence

ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGTTCCCTCATCACAATCCCCCTCACCACCTTCCCTTCAATTCCAGTTACAGATCTATGGCAGAACATTAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAATCCAAACAGTTCCATCGAGAAAGTCTCTATGTTCTCTCGTAGCTATGTAGCTTATTCAATCTCCCTCAGCTTTGGAACTCCATCGCAGAATTTGTTGTTCGTGTTCGATACTGGAAGTAGCCTCGTCTGGTTCCCCTACACTGTCGGTTGTCTCTGTTCCAATTCTTCGTTTCCGAACTCAGACGATTGCGAAGTTTATCCCCAAATTATCTTCCTCTGTGAAGATCGTCGGTTGCTGAAATCGACGGAAAGGTGA

Coding sequence (CDS)

ATGGAGTTTCTCCCCATTCCCTTTCTCTTTTCCATCTTTCTCCTTCTTTCCACTTCATCTTCTTCCTCCAGTTCCCTCATCACAATCCCCCTCACCACCTTCCCTTCAATTCCAGTTACAGATCTATGGCAGAACATTAATTACCTCGTCTCTGCTTCACTCACCAGAGCTCATCACCTCAAGAGGCCGCGAACGAATCCAAACAGTTCCATCGAGAAAGTCTCTATGTTCTCTCGTAGCTATGTAGCTTATTCAATCTCCCTCAGCTTTGGAACTCCATCGCAGAATTTGTTGTTCGTGTTCGATACTGGAAGTAGCCTCGTCTGGTTCCCCTACACTGTCGGTTGTCTCTGTTCCAATTCTTCGTTTCCGAACTCAGACGATTGCGAAGTTTATCCCCAAATTATCTTCCTCTGTGAAGATCGTCGGTTGCTGAAATCGACGGAAAGGTGA

Protein sequence

MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVTDLWQNINYLVSASLTRAHHLKRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCSNSSFPNSDDCEVYPQIIFLCEDRRLLKSTER
Homology
BLAST of Lag0011762 vs. NCBI nr
Match: XP_023543736.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 169.9 bits (429), Expect = 1.7e-38
Identity = 88/127 (69.29%), Postives = 100/127 (78.74%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVTDLWQNINYLVSASLTRAHHL 60
           MEF PIPFL SI LLLS SSSSSS+ +T+PLT FPS+P T  W+NI +LVSASLTRA HL
Sbjct: 1   MEFFPIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFTHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCSN 120
           K PR   N+SI+ V++F RSY AYSISL+FGTP Q+L  VFDTGSSLVWFP T G  CSN
Sbjct: 61  KTPRIKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 SSFPNSD 128
            SFPN D
Sbjct: 121 CSFPNVD 127

BLAST of Lag0011762 vs. NCBI nr
Match: XP_022979057.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 168.3 bits (425), Expect = 5.0e-38
Identity = 90/150 (60.00%), Postives = 106/150 (70.67%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVTDLWQNINYLVSASLTRAHHL 60
           MEF PI FL SI LLLS SSSSSS  +T+PLT FPS+P+T  W+NI +LVSASL RA HL
Sbjct: 1   MEFFPIQFLLSIVLLLSASSSSSSITVTLPLTAFPSLPLTHPWKNIKHLVSASLARAQHL 60

Query: 61  KRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCSN 120
           K P+T  N+SI+ V++F RSY AYSISL+FGTP Q+L  VFDTGSSLVWFP T G  CSN
Sbjct: 61  KTPKTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 SSFPNSDDCEVYPQIIFLCEDRRLLKSTER 151
            SFPN D   +   I  L    R++    R
Sbjct: 121 CSFPNVDAATIPKFIPKLSSSARIIGCRNR 150

BLAST of Lag0011762 vs. NCBI nr
Match: XP_022925946.1 (probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic proteinase nepenthesin-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 166.4 bits (420), Expect = 1.9e-37
Identity = 87/127 (68.50%), Postives = 99/127 (77.95%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVTDLWQNINYLVSASLTRAHHL 60
           MEF  IPFL SI LLLS SSSSSS+ +T+PLT FPS+P    W+NI +LVSASLTRA HL
Sbjct: 1   MEFFLIPFLLSIVLLLSASSSSSSTTVTLPLTVFPSLPFAHPWKNIKHLVSASLTRAQHL 60

Query: 61  KRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCSN 120
           K PRT  N+SI+ V++F RSY AYSISL+FGTP Q+L  VFDTGSSLVWFP T G  CSN
Sbjct: 61  KTPRTKSNTSIQNVALFPRSYGAYSISLAFGTPPQSLSLVFDTGSSLVWFPCTAGYRCSN 120

Query: 121 SSFPNSD 128
            SFPN D
Sbjct: 121 CSFPNVD 127

BLAST of Lag0011762 vs. NCBI nr
Match: XP_038881211.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 166.0 bits (419), Expect = 2.5e-37
Identity = 93/128 (72.66%), Postives = 102/128 (79.69%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVT-DLWQNINYLVSASLTRAHH 60
           MEF PIPFLFSIFLLL TSSSSS S IT+PLT FPSIP+T D  + INYL+SASL RA H
Sbjct: 1   MEFFPIPFLFSIFLLLPTSSSSSISTITLPLTAFPSIPLTDDPLKIINYLLSASLNRAQH 60

Query: 61  LKRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCS 120
           LK P+T    SI+ VS+FSRSY AYSI+L+FGTP QNL FVFDTGSSLVWFP T G  CS
Sbjct: 61  LKNPQT---KSIQNVSLFSRSYGAYSITLAFGTPPQNLSFVFDTGSSLVWFPCTAGYRCS 120

Query: 121 NSSFPNSD 128
           N SFPN D
Sbjct: 121 NCSFPNVD 125

BLAST of Lag0011762 vs. NCBI nr
Match: TYK07446.1 (aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 165.6 bits (418), Expect = 3.2e-37
Identity = 87/131 (66.41%), Postives = 104/131 (79.39%), Query Frame = 0

Query: 1   MEFLPIPFLFSIFLLLSTSSSSSSSLITIPLTTFPSIPVTDLWQNINYLVSASLTRAHHL 60
           MEFLPIPFLFSIFLLL TSSSSS   IT+PLTTFPSIP TD  + IN+L+SASL+RA HL
Sbjct: 1   MEFLPIPFLFSIFLLLPTSSSSS---ITLPLTTFPSIPFTDPLKTINHLLSASLSRAQHL 60

Query: 61  KRPRTNPNSSIEKVSMFSRSYVAYSISLSFGTPSQNLLFVFDTGSSLVWFPYTVGCLCSN 120
           K P++  N+S E VS+F RSY AY++SL+FGTP QNL F+FDTGSSLVWFP T G  C++
Sbjct: 61  KSPQSKSNTSTENVSLFPRSYGAYAVSLAFGTPPQNLSFIFDTGSSLVWFPCTAGYRCAH 120

Query: 121 SSFPNSDDCEV 132
            SFP+ D   +
Sbjct: 121 CSFPHVDPATI 128

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023543736.11.7e-3869.29probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo][more]
XP_022979057.15.0e-3860.00probable aspartyl protease At4g16563 [Cucurbita maxima][more]
XP_022925946.11.9e-3768.50probable aspartyl protease At4g16563 [Cucurbita moschata] >KAG6604319.1 Aspartic... [more]
XP_038881211.12.5e-3772.66probable aspartyl protease At4g16563 [Benincasa hispida][more]
TYK07446.13.2e-3766.41aspartic proteinase nepenthesin-2-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 41..141
e-value: 2.6E-10
score: 42.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 9..119
IPR033121Peptidase family A1 domainPFAMPF00026Aspcoord: 84..118
e-value: 1.3E-5
score: 24.8
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 84..150
score: 12.326554
NoneNo IPR availablePANTHERPTHR13683:SF712ASPARTIC PROTEINASE NEPENTHESIN-2-LIKEcoord: 10..129
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 10..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011762.1Lag0011762.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity