Lag0011667 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011667
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Polygalacturonase-inhibiting protein
Location	chr1: 30217521 .. 30217913 (-)
RNA-Seq Expression	Lag0011667
Synteny	Lag0011667

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCACAAGCTCCCGAATCTCACCGGCCCCATCCAACCCTCCATTGCAAAGCTTCTCAACCTCAAATTCTTTTGGATTAACTGGACCAACATTTCTGGCTCGATCCATGATTTCCTCAGCACCCTCACCAATCTCAACCACCTCAGCCTCTCGTTTAACTTGTCGGAGGTTGGGTTTCCTCGGAGCTTGACTTCGTTGGATGTTAATCATAATAAGGTATTTGGGGGTTTGCCGCAGGAGATAGCGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTTTGTGGTGAGATTCCGGTGGGTGGAGAGTTGCTGAGGTTTGATGTGTACTCGTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTAGTAGCTGTATATGA

mRNA sequence

ATGCTTCACAAGCTCCCGAATCTCACCGGCCCCATCCAACCCTCCATTGCAAAGCTTCTCAACCTCAAATTCTTTTGGATTAACTGGACCAACATTTCTGGCTCGATCCATGATTTCCTCAGCACCCTCACCAATCTCAACCACCTCAGCCTCTCGTTTAACTTGTCGGAGGTTGGGTTTCCTCGGAGCTTGACTTCGTTGGATGTTAATCATAATAAGGTATTTGGGGGTTTGCCGCAGGAGATAGCGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTTTGTGGTGAGATTCCGGTGGGTGGAGAGTTGCTGAGGTTTGATGTGTACTCGTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTAGTAGCTGTATATGA

Coding sequence (CDS)

ATGCTTCACAAGCTCCCGAATCTCACCGGCCCCATCCAACCCTCCATTGCAAAGCTTCTCAACCTCAAATTCTTTTGGATTAACTGGACCAACATTTCTGGCTCGATCCATGATTTCCTCAGCACCCTCACCAATCTCAACCACCTCAGCCTCTCGTTTAACTTGTCGGAGGTTGGGTTTCCTCGGAGCTTGACTTCGTTGGATGTTAATCATAATAAGGTATTTGGGGGTTTGCCGCAGGAGATAGCGAAGCTGAATTTTCAGTATTTGAATGTGAGTTATAATAGGCTTTGTGGTGAGATTCCGGTGGGTGGAGAGTTGCTGAGGTTTGATGTGTACTCGTATTTTCACAATAAGTGTTTGTGCGGCGCACCGCTTAGTAGCTGTATATGA

Protein sequence

MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLSFNLSEVGFPRSLTSLDVNHNKVFGGLPQEIAKLNFQYLNVSYNRLCGEIPVGGELLRFDVYSYFHNKCLCGAPLSSCI

Homology

[Close]

BLAST of Lag0011667 vs. NCBI nr
Match: XP_023517351.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-40
Identity = 104/231 (45.02%), Postives = 113/231 (48.92%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS-------- 60
           MLHKLPNLTGPI P+IAKL NLKF  ++W ++SG I DFLSTL+NL  LSLS        
Sbjct: 99  MLHKLPNLTGPIHPNIAKLHNLKFLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTI 158

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 159 PISLSKLPNLGGLELDRNNLTGEIPDSFGYFTGEQAPSLYLSHNQLSGKIPSSLSRLNFT 218

Query: 121 ----------------------------------FNLSEVGFPRSLTSLDVNHNKVFGGL 130
                                             FNLSE  FP+SLTSLDVNHNKVFGGL
Sbjct: 219 SIDLSRNKLKGDAFMIFGSNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGL 278

[Close]

BLAST of Lag0011667 vs. NCBI nr
Match: KAG6595658.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 174.1 bits (440), Expect = 7.9e-40
Identity = 102/231 (44.16%), Postives = 113/231 (48.92%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS-------- 60
           +LHKLPNLTGPI PSIAKL NLK+  ++W ++SG I DFLSTL+NL  LSLS        
Sbjct: 101 ILHKLPNLTGPIHPSIAKLHNLKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTI 160

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 161 PNSLSKLPNLGGLELDRNNLTGEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFT 220

Query: 121 ----------------------------------FNLSEVGFPRSLTSLDVNHNKVFGGL 130
                                             FNLSE  FP+SLTSLD+NHNKVFGGL
Sbjct: 221 SIDLSRNKLKGDAFMIFGLNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDLNHNKVFGGL 280

[Close]

BLAST of Lag0011667 vs. NCBI nr
Match: XP_022966389.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 171.8 bits (434), Expect = 3.9e-39
Identity = 102/231 (44.16%), Postives = 112/231 (48.48%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS-------- 60
           +LHKLPNLTGPI P+IAKL NLKF  ++W ++SG I DFLSTL+NL  LSLS        
Sbjct: 98  ILHKLPNLTGPIHPNIAKLHNLKFLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTI 157

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 158 PNSLSKLPNLGGLQLDRNNLTGEIPDSFGYFTGEQAPSLYLSHNQLSGKIPSSLSRLNFT 217

Query: 121 ----------------------------------FNLSEVGFPRSLTSLDVNHNKVFGGL 130
                                             FNLSE  FP+SLTSLDVNHNKVFGGL
Sbjct: 218 SIDLSRNKLKGDAFMIFGSNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGL 277

[Close]

BLAST of Lag0011667 vs. NCBI nr
Match: XP_022925134.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 170.6 bits (431), Expect = 8.7e-39
Identity = 101/231 (43.72%), Postives = 112/231 (48.48%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS-------- 60
           +LHKLPNLTGPI P+IAKL NLK+  ++W ++SG I DFLSTL+NL  LSLS        
Sbjct: 101 ILHKLPNLTGPIHPNIAKLHNLKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTI 160

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 161 PNSLSKLPNLGGLELDRNNLTGEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFT 220

Query: 121 ----------------------------------FNLSEVGFPRSLTSLDVNHNKVFGGL 130
                                             FNLSE  FP+SLTSLDVNHNKVFGGL
Sbjct: 221 SIDLSRNKLKGDAFMIFGSNKTVQIVDLSRNQLEFNLSEAVFPQSLTSLDVNHNKVFGGL 280

[Close]

BLAST of Lag0011667 vs. NCBI nr
Match: KAB2608146.1 (Polygalacturonase-inhibiting protein [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 90/213 (42.25%), Postives = 105/213 (49.30%), Query Frame = 0


Query: 3   HKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS---------- 62
           HK PNLTGPIQP+IAKL  LK   ++WTN+SGS+ DFLS L NL  L LS          
Sbjct: 130 HKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPS 189

Query: 63  ------------------------------------------------------------ 122
                                                                       
Sbjct: 190 SLSELPNLGALRLDRNKLTDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFG 249

Query: 123 ----------------FNLSEVGFPRSLTSLDVNHNKVFGGLPQEIAKLNFQYLNVSYNR 130
                           FNLS+V FP SLTSLD+NHNK++G +P E  +LNFQ+LNVSYNR
Sbjct: 250 LNKTTQIVDLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNR 309

[Close]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 2.2e-37
Identity = 90/228 (39.47%), Postives = 105/228 (46.05%), Query Frame = 0


Query: 3   HKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLS---------- 62
           HK PNLTGPIQP+IAKL  LK   ++WTN+SGS+ DFLS L NL  L LS          
Sbjct: 102 HKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPS 161

Query: 63  ------------------------------------------------------------ 122
                                                                       
Sbjct: 162 SLSELPNLGALRLDRNKLTGHIPISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSID 221

Query: 123 -------------------------------FNLSEVGFPRSLTSLDVNHNKVFGGLPQE 130
                                          FNLS+V FP SLTSLD+NHNK++G +P E
Sbjct: 222 LSRNKLEGDASVIFGLNKTTQIVDLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVE 281

[Close]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.3e-29
Identity = 80/230 (34.78%), Postives = 99/230 (43.04%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLSFN------ 60
           +  KL NLTG I P+IAKL +LK   ++WTN+SG +  F S L NL +L LSFN      
Sbjct: 103 IFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFSELKNLTYLDLSFNNLSGPI 162

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 163 PGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNV 222

Query: 121 -----------------------------------LSEVGFPRSLTSLDVNHNKVFGGLP 130
                                              LS V FP+SLTSLD++HNK+ G LP
Sbjct: 223 MDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLP 282

[Close]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 3.6e-27
Identity = 76/230 (33.04%), Postives = 96/230 (41.74%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLSFN------ 60
           +  KL NLTG IQP+IAKL NL F  ++WTN++G + +FLS L NL ++ LSFN      
Sbjct: 100 IFRKLTNLTGHIQPTIAKLKNLTFLRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSI 159

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 160 PSSLSSLRKLEYLELSRNKLTGPIPESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYR 219

Query: 121 -----------------------------------LSEVGFPRSLTSLDVNHNKVFGGLP 130
                                              LS+V   ++L +LD+NHN + G +P
Sbjct: 220 IDLSRNKLQGDASILFGAKKTTWIVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIP 279

[Close]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.0e-26
Identity = 73/230 (31.74%), Postives = 93/230 (40.43%), Query Frame = 0


Query: 1   MLHKLPNLTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLNHLSLSFN------ 60
           +  KL NLTG IQP+IAKL NL+   ++WTN++G I DF+S L NL  L LSFN      
Sbjct: 100 VFRKLSNLTGTIQPTIAKLKNLRMLRLSWTNLTGPIPDFISQLKNLEFLELSFNDLSGSI 159

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 160 PSSLSTLPKILALELSRNKLTGSIPESFGSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNR 219

Query: 121 -----------------------------------LSEVGFPRSLTSLDVNHNKVFGGLP 130
                                              +S+V  P++L  LD+NHN + G +P
Sbjct: 220 IDLSRNKLQGDASMLFGSNKTTWSIDLSRNMFQFDISKVDIPKTLGILDLNHNGITGNIP 279

[Close]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot
Match: P35334 (Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.2e-24
Identity = 61/128 (47.66%), Postives = 81/128 (63.28%), Query Frame = 0


Query: 8   LTGPIQPSIAKLLNLKFFWINWTNISGSIHDFLSTLTNLN--HL---SLSFNLSEVGFPR 67
           LTG I P+ A  LNL F  ++   + G       +  N    HL   SL+F+L +VG  +
Sbjct: 215 LTGKIPPTFAN-LNLAFVDLSRNMLEGDASVLFGSDKNTKKIHLAKNSLAFDLGKVGLSK 274

Query: 68  SLTSLDVNHNKVFGGLPQEIAKLNF-QYLNVSYNRLCGEIPVGGELLRFDVYSYFHNKCL 127
           +L  LD+ +N+++G LPQ + +L F Q LNVS+N LCGEIP GG L RFDV SY +NKCL
Sbjct: 275 NLNGLDLRNNRIYGTLPQGLTQLKFLQSLNVSFNNLCGEIPQGGNLKRFDVSSYANNKCL 334

Query: 128 CGAPLSSC 130
           CG+PL SC
Sbjct: 335 CGSPLPSC 341

The following BLAST results are available for this feature:

BLAST of Lag0011667 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517351.1	1.2e-40	45.02	polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
KAG6595658.1	7.9e-40	44.16	Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022966389.1	3.9e-39	44.16	polygalacturonase inhibitor-like [Cucurbita maxima]	[more]
XP_022925134.1	8.7e-39	43.72	polygalacturonase inhibitor-like [Cucurbita moschata]	[more]
KAB2608146.1	3.1e-36	42.25	Polygalacturonase-inhibiting protein [Pyrus ussuriensis x Pyrus communis]	[more]

BLAST of Lag0011667 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05091	2.2e-37	39.47	Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1	[more]
A7PW81	1.3e-29	34.78	Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1	[more]
Q9M5J8	3.6e-27	33.04	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2	[more]
Q9M5J9	3.0e-26	31.74	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1	[more]
P35334	8.2e-24	47.66	Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 4..59 e-value: 1.6E-8 score: 36.5
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 60..130 e-value: 2.6E-17 score: 64.2
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 53..129
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 2..67
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 2..67
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 53..129
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 6..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0011667.1	Lag0011667.1	mRNA