Lag0011458 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011458
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H
Locationchr1: 25279305 .. 25279901 (-)
RNA-Seq ExpressionLag0011458
SyntenyLag0011458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAGGGCGAGGTTGACCAAAGATGGCCGAGACAAACAAGAACCCCACGAGAGATCAGAACCATCTTTGGAGGACCAGCTGGAGGAGGTTCAAGTAGGAAAAGGAAAGCTATTGCCAGAGAAGCAAGGTTCGAACCAGAATATCGAGGTATATACTCTGTCCATCTAGCAAAGACACACCCCCCTTTGGAGTTCACTGAGGCCGAGGCAACAAGCATTCATCAGCCACATAATGATGCTCTGGTGGTCACTCTAATGGTAGCCAATGTGAAAATCCATCGGATCCTAATTGATGGAGAAAGCTCGGCTGATGTCCTTTCTCTAACAGCCTTTAAAGCAATGCAGTTAGGGAGAGAACTCTTAAAGCCAAGTCTTACCCCACTTGTAGGATTTGGAGGAGAAAGAGTGACCCCAGAGGGTAGCATAGAATTTTCAGTGATCTTTGGGGATGGACAGAATACAGTAACCAAGATAATAAACTTCCTAGTCGTGGATTGTATGCCAGCCTACAATGCAATCCTAGGAAGGCCAACCTTGCATGAAATGAAGGCCATAGCTTCAACTCACCATCAGTTGCTGAAGTTTCCAACTTAG

mRNA sequence

ATGGCCGAGGGCGAGGTTGACCAAAGATGGCCGAGACAAACAAGAACCCCACGAGAGATCAGAACCATCTTTGGAGGACCAGCTGGAGGAGGTTCAAGTAGGAAAAGGAAAGCTATTGCCAGAGAAGCAAGGTTCGAACCAGAATATCGAGGTATATACTCTGTCCATCTAGCAAAGACACACCCCCCTTTGGAGTTCACTGAGGCCGAGGCAACAAGCATTCATCAGCCACATAATGATGCTCTGGTGGTCACTCTAATGGTAGCCAATGTGAAAATCCATCGGATCCTAATTGATGGAGAAAGCTCGGCTGATGTCCTTTCTCTAACAGCCTTTAAAGCAATGCAGTTAGGGAGAGAACTCTTAAAGCCAAGTCTTACCCCACTTGTAGGATTTGGAGGAGAAAGAGTGACCCCAGAGGGTAGCATAGAATTTTCAGTGATCTTTGGGGATGGACAGAATACAGTAACCAAGATAATAAACTTCCTAGTCGTGGATTGTATGCCAGCCTACAATGCAATCCTAGGAAGGCCAACCTTGCATGAAATGAAGGCCATAGCTTCAACTCACCATCAGTTGCTGAAGTTTCCAACTTAG

Coding sequence (CDS)

ATGGCCGAGGGCGAGGTTGACCAAAGATGGCCGAGACAAACAAGAACCCCACGAGAGATCAGAACCATCTTTGGAGGACCAGCTGGAGGAGGTTCAAGTAGGAAAAGGAAAGCTATTGCCAGAGAAGCAAGGTTCGAACCAGAATATCGAGGTATATACTCTGTCCATCTAGCAAAGACACACCCCCCTTTGGAGTTCACTGAGGCCGAGGCAACAAGCATTCATCAGCCACATAATGATGCTCTGGTGGTCACTCTAATGGTAGCCAATGTGAAAATCCATCGGATCCTAATTGATGGAGAAAGCTCGGCTGATGTCCTTTCTCTAACAGCCTTTAAAGCAATGCAGTTAGGGAGAGAACTCTTAAAGCCAAGTCTTACCCCACTTGTAGGATTTGGAGGAGAAAGAGTGACCCCAGAGGGTAGCATAGAATTTTCAGTGATCTTTGGGGATGGACAGAATACAGTAACCAAGATAATAAACTTCCTAGTCGTGGATTGTATGCCAGCCTACAATGCAATCCTAGGAAGGCCAACCTTGCATGAAATGAAGGCCATAGCTTCAACTCACCATCAGTTGCTGAAGTTTCCAACTTAG

Protein sequence

MAEGEVDQRWPRQTRTPREIRTIFGGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVVTLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIEFSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT
Homology
BLAST of Lag0011458 vs. NCBI nr
Match: XP_022155186.1 (uncharacterized protein LOC111022321 [Momordica charantia])

HSP 1 Score: 196.4 bits (498), Expect = 2.3e-46
Identity = 99/173 (57.23%), Postives = 124/173 (71.68%), Query Frame = 0

Query: 26  GPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVVT 85
           GP    S RKRKA  REAR   +   +Y  ++      +EF+E EAT +  PHNDALV+T
Sbjct: 3   GPIERESERKRKAYVREARTSRKQNEVYHAYITNQQVTIEFSENEATHLLHPHNDALVIT 62

Query: 86  LMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIEF 145
           L +ANVK+HRIL+DG SSAD++S TA+KAM LG ++LK S  PLVGFGGERV  EG IE 
Sbjct: 63  LKIANVKVHRILVDGGSSADIISWTAYKAMDLGEKVLKSSPAPLVGFGGERVILEGRIEL 122

Query: 146 SVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
            V FG G  +VTK+++FLVV+   +YNAILGRPT+H +KAI ST+HQ  KFPT
Sbjct: 123 PVTFGSGPKSVTKMVDFLVVNYTSSYNAILGRPTMHMLKAIPSTYHQSXKFPT 175

BLAST of Lag0011458 vs. NCBI nr
Match: XP_022158093.1 (uncharacterized protein LOC111024662 [Momordica charantia])

HSP 1 Score: 196.1 bits (497), Expect = 3.0e-46
Identity = 97/181 (53.59%), Postives = 125/181 (69.06%), Query Frame = 0

Query: 18  REIRTIFGGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQP 77
           +EI+TIFGGP      +KRKA  +EAR  P Y  +Y          ++F+E E T +  P
Sbjct: 65  KEIQTIFGGPTERELGKKRKASMQEARASPGYCKVYHASFTGQPSKIKFSEDETTRLFHP 124

Query: 78  HNDALVVTLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERV 137
           HNDALV+TL + N K+HRIL+DG SS  ++SLTA+KAM LG + LK +L PLVGFGGERV
Sbjct: 125 HNDALVITLKITNTKVHRILVDGGSSTGIISLTAYKAMDLGEKALKSNLAPLVGFGGERV 184

Query: 138 TPEGSIEFSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFP 197
             +  I+  V FG+G   +TKI+ FLVVD   +YNAILGRPT+H +KAI ST+H+ LKFP
Sbjct: 185 IQKCRIKLPVTFGNGPRNITKIVEFLVVDYSSSYNAILGRPTIHTLKAIPSTYHKSLKFP 244

Query: 198 T 199
           T
Sbjct: 245 T 245

BLAST of Lag0011458 vs. NCBI nr
Match: XP_022158687.1 (uncharacterized protein LOC111025147 [Momordica charantia])

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-45
Identity = 98/174 (56.32%), Postives = 123/174 (70.69%), Query Frame = 0

Query: 25  GGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVV 84
           GGP    S RKRKA  REAR       +Y  ++A     +EF+E EAT +  PHNDALV+
Sbjct: 2   GGPIKRESGRKRKADVREARASLRQNKVYHAYIADRSVTIEFSEDEATHLLHPHNDALVI 61

Query: 85  TLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIE 144
           TL +ANVK+HRIL+DG SSAD++S T +K M LG ++LK S  PL+GFG ERV PEG IE
Sbjct: 62  TLKIANVKVHRILVDGGSSADIISWTTYKVMDLGEKVLKSSPAPLIGFGRERVIPEGRIE 121

Query: 145 FSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
             V FG G  +VTK+++FLVVD   +YNAILGR T+H +KAI ST+HQ +KFPT
Sbjct: 122 LHVTFGSGPKSVTKMMDFLVVDYTSSYNAILGRSTMHMLKAIPSTYHQSMKFPT 175

BLAST of Lag0011458 vs. NCBI nr
Match: XP_022150028.1 (uncharacterized protein LOC111018300 [Momordica charantia])

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-45
Identity = 104/195 (53.33%), Postives = 127/195 (65.13%), Query Frame = 0

Query: 4   GEVDQRWPRQTRTPREIRTIFGGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPP 63
           GE D + P      REIRTI GGP      RKRKA  RE R       IY   +AK    
Sbjct: 78  GENDSKSP-----AREIRTIMGGPMEREFGRKRKASIRETRTSQVQHEIYHTFVAKQPAK 137

Query: 64  LEFTEAEATSIHQPHNDALVVTLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLK 123
           +EF+E EAT +  PHND LV+TL +AN K+HRIL+DG SSAD++SLTA+KAM LG    K
Sbjct: 138 IEFSEDEATHLLHPHNDVLVITLKIANAKVHRILVDGGSSADIISLTAYKAMDLGERGRK 197

Query: 124 PSLTPLVGFGGERVTPEGSIEFSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEM 183
            S   LV F GERV PEG  E +V FG G  ++T I++FLV+D   +YNAILGRPT+H +
Sbjct: 198 SSPASLVRFRGERVIPEGRTELTVTFGSGPKSITTIMDFLVIDYASSYNAILGRPTIHML 257

Query: 184 KAIASTHHQLLKFPT 199
           KAI ST+HQ + FPT
Sbjct: 258 KAIPSTYHQSINFPT 267

BLAST of Lag0011458 vs. NCBI nr
Match: XP_022146774.1 (uncharacterized protein LOC111015901 [Momordica charantia])

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-44
Identity = 93/173 (53.76%), Postives = 122/173 (70.52%), Query Frame = 0

Query: 26  GPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVVT 85
           GP    S RKRKA  REAR       +Y  ++A     +EF+E EAT +  PHNDALV+T
Sbjct: 3   GPIERESGRKRKANVREARASLGQSEVYHAYVADRSMKIEFSEGEATHVLHPHNDALVIT 62

Query: 86  LMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIEF 145
           L +AN K+HRIL+DG SS D+ SLTA+KAM LG E+LK SLTPL+GFGGER+ P+G IE 
Sbjct: 63  LKIANAKVHRILVDGGSSTDISSLTAYKAMDLGEEVLKSSLTPLIGFGGERIIPKGRIEL 122

Query: 146 SVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
            + F     ++T++++FLVVD   +YN IL RPT+H ++AI ST+HQ +KFPT
Sbjct: 123 PITFRSEPKSITRMVDFLVVDYTSSYNTILERPTMHMLRAIPSTYHQSMKFPT 175

BLAST of Lag0011458 vs. ExPASy TrEMBL
Match: A0A6J1DQY2 (uncharacterized protein LOC111022321 OS=Momordica charantia OX=3673 GN=LOC111022321 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.1e-46
Identity = 99/173 (57.23%), Postives = 124/173 (71.68%), Query Frame = 0

Query: 26  GPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVVT 85
           GP    S RKRKA  REAR   +   +Y  ++      +EF+E EAT +  PHNDALV+T
Sbjct: 3   GPIERESERKRKAYVREARTSRKQNEVYHAYITNQQVTIEFSENEATHLLHPHNDALVIT 62

Query: 86  LMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIEF 145
           L +ANVK+HRIL+DG SSAD++S TA+KAM LG ++LK S  PLVGFGGERV  EG IE 
Sbjct: 63  LKIANVKVHRILVDGGSSADIISWTAYKAMDLGEKVLKSSPAPLVGFGGERVILEGRIEL 122

Query: 146 SVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
            V FG G  +VTK+++FLVV+   +YNAILGRPT+H +KAI ST+HQ  KFPT
Sbjct: 123 PVTFGSGPKSVTKMVDFLVVNYTSSYNAILGRPTMHMLKAIPSTYHQSXKFPT 175

BLAST of Lag0011458 vs. ExPASy TrEMBL
Match: A0A6J1DV51 (uncharacterized protein LOC111024662 OS=Momordica charantia OX=3673 GN=LOC111024662 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.4e-46
Identity = 97/181 (53.59%), Postives = 125/181 (69.06%), Query Frame = 0

Query: 18  REIRTIFGGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQP 77
           +EI+TIFGGP      +KRKA  +EAR  P Y  +Y          ++F+E E T +  P
Sbjct: 65  KEIQTIFGGPTERELGKKRKASMQEARASPGYCKVYHASFTGQPSKIKFSEDETTRLFHP 124

Query: 78  HNDALVVTLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERV 137
           HNDALV+TL + N K+HRIL+DG SS  ++SLTA+KAM LG + LK +L PLVGFGGERV
Sbjct: 125 HNDALVITLKITNTKVHRILVDGGSSTGIISLTAYKAMDLGEKALKSNLAPLVGFGGERV 184

Query: 138 TPEGSIEFSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFP 197
             +  I+  V FG+G   +TKI+ FLVVD   +YNAILGRPT+H +KAI ST+H+ LKFP
Sbjct: 185 IQKCRIKLPVTFGNGPRNITKIVEFLVVDYSSSYNAILGRPTIHTLKAIPSTYHKSLKFP 244

Query: 198 T 199
           T
Sbjct: 245 T 245

BLAST of Lag0011458 vs. ExPASy TrEMBL
Match: A0A6J1D8C9 (uncharacterized protein LOC111018300 OS=Momordica charantia OX=3673 GN=LOC111018300 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.4e-46
Identity = 104/195 (53.33%), Postives = 127/195 (65.13%), Query Frame = 0

Query: 4   GEVDQRWPRQTRTPREIRTIFGGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPP 63
           GE D + P      REIRTI GGP      RKRKA  RE R       IY   +AK    
Sbjct: 78  GENDSKSP-----AREIRTIMGGPMEREFGRKRKASIRETRTSQVQHEIYHTFVAKQPAK 137

Query: 64  LEFTEAEATSIHQPHNDALVVTLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLK 123
           +EF+E EAT +  PHND LV+TL +AN K+HRIL+DG SSAD++SLTA+KAM LG    K
Sbjct: 138 IEFSEDEATHLLHPHNDVLVITLKIANAKVHRILVDGGSSADIISLTAYKAMDLGERGRK 197

Query: 124 PSLTPLVGFGGERVTPEGSIEFSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEM 183
            S   LV F GERV PEG  E +V FG G  ++T I++FLV+D   +YNAILGRPT+H +
Sbjct: 198 SSPASLVRFRGERVIPEGRTELTVTFGSGPKSITTIMDFLVIDYASSYNAILGRPTIHML 257

Query: 184 KAIASTHHQLLKFPT 199
           KAI ST+HQ + FPT
Sbjct: 258 KAIPSTYHQSINFPT 267

BLAST of Lag0011458 vs. ExPASy TrEMBL
Match: A0A6J1DXV4 (uncharacterized protein LOC111025147 OS=Momordica charantia OX=3673 GN=LOC111025147 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 5.4e-46
Identity = 98/174 (56.32%), Postives = 123/174 (70.69%), Query Frame = 0

Query: 25  GGPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVV 84
           GGP    S RKRKA  REAR       +Y  ++A     +EF+E EAT +  PHNDALV+
Sbjct: 2   GGPIKRESGRKRKADVREARASLRQNKVYHAYIADRSVTIEFSEDEATHLLHPHNDALVI 61

Query: 85  TLMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIE 144
           TL +ANVK+HRIL+DG SSAD++S T +K M LG ++LK S  PL+GFG ERV PEG IE
Sbjct: 62  TLKIANVKVHRILVDGGSSADIISWTTYKVMDLGEKVLKSSPAPLIGFGRERVIPEGRIE 121

Query: 145 FSVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
             V FG G  +VTK+++FLVVD   +YNAILGR T+H +KAI ST+HQ +KFPT
Sbjct: 122 LHVTFGSGPKSVTKMMDFLVVDYTSSYNAILGRSTMHMLKAIPSTYHQSMKFPT 175

BLAST of Lag0011458 vs. ExPASy TrEMBL
Match: A0A6J1CZ14 (uncharacterized protein LOC111015901 OS=Momordica charantia OX=3673 GN=LOC111015901 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 1.0e-44
Identity = 93/173 (53.76%), Postives = 122/173 (70.52%), Query Frame = 0

Query: 26  GPAGGGSSRKRKAIAREARFEPEYRGIYSVHLAKTHPPLEFTEAEATSIHQPHNDALVVT 85
           GP    S RKRKA  REAR       +Y  ++A     +EF+E EAT +  PHNDALV+T
Sbjct: 3   GPIERESGRKRKANVREARASLGQSEVYHAYVADRSMKIEFSEGEATHVLHPHNDALVIT 62

Query: 86  LMVANVKIHRILIDGESSADVLSLTAFKAMQLGRELLKPSLTPLVGFGGERVTPEGSIEF 145
           L +AN K+HRIL+DG SS D+ SLTA+KAM LG E+LK SLTPL+GFGGER+ P+G IE 
Sbjct: 63  LKIANAKVHRILVDGGSSTDISSLTAYKAMDLGEEVLKSSLTPLIGFGGERIIPKGRIEL 122

Query: 146 SVIFGDGQNTVTKIINFLVVDCMPAYNAILGRPTLHEMKAIASTHHQLLKFPT 199
            + F     ++T++++FLVVD   +YN IL RPT+H ++AI ST+HQ +KFPT
Sbjct: 123 PITFRSEPKSITRMVDFLVVDYTSSYNTILERPTMHMLRAIPSTYHQSMKFPT 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155186.12.3e-4657.23uncharacterized protein LOC111022321 [Momordica charantia][more]
XP_022158093.13.0e-4653.59uncharacterized protein LOC111024662 [Momordica charantia][more]
XP_022158687.11.1e-4556.32uncharacterized protein LOC111025147 [Momordica charantia][more]
XP_022150028.11.1e-4553.33uncharacterized protein LOC111018300 [Momordica charantia][more]
XP_022146774.12.1e-4453.76uncharacterized protein LOC111015901 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DQY21.1e-4657.23uncharacterized protein LOC111022321 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1DV511.4e-4653.59uncharacterized protein LOC111024662 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1D8C95.4e-4653.33uncharacterized protein LOC111018300 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1DXV45.4e-4656.32uncharacterized protein LOC111025147 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1CZ141.0e-4453.76uncharacterized protein LOC111015901 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 66..198
e-value: 4.2E-8
score: 34.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 84..181
e-value: 5.51313E-14
score: 62.7392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011458.1Lag0011458.1mRNA