Lag0011456 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0011456 vs. NCBI nr
Match: XP_022928703.1 (aspartic proteinase nepenthesin-1 [Cucurbita moschata])

HSP 1 Score: 769.2 bits (1985), Expect = 2.0e-218
Identity = 384/451 (85.14%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 7   SIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFER 66
           S+RY I+ VVL  T+    +SA SS+LSRRAL +P+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDT 126
           LQRGV RG+ RL RLNAMVLAAN  VG +V+APVVAGNGEFLMKLAIGSPPR FSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPC+QCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLL+GSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPVDDS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IFGNLQQQN MVVHDLQEET+SFLPT+C  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

BLAST of Lag0011456 vs. NCBI nr
Match: KAG7033562.1 (Aspartic proteinase nepenthesin-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.7 bits (1981), Expect = 5.7e-218
Identity = 384/451 (85.14%), Postives = 412/451 (91.35%), Query Frame = 0

Query: 7   SIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFER 66
           S+RY I+ VVL  T+    +SA SS+LSRRAL +P+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +V+APVVAGNGEFLMKLAIGSPPR FSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPC+QCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S+ E+KTTPLI NPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASEGEIKTTPLISNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPVDDS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDSRA LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSRAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IFGNLQQQN MVVHDLQEET+SFLPT+C  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Lag0011456 vs. NCBI nr
Match: XP_023543561.1 (aspartic proteinase nepenthesin-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.3 bits (1980), Expect = 7.4e-218
Identity = 384/451 (85.14%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 7   SIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFER 66
           S+RY IV VVL  T+    +SA SS+ SRRAL++P+ PSDGFRV LNHVDHVKNLTRFE+
Sbjct: 4   SLRYLIVSVVLSITMLFIHTSASSSSHSRRALRQPKLPSDGFRVSLNHVDHVKNLTRFEQ 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +V+APVVAGNGEFLMKLAIGSPPR FSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPC+QCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPVDDS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDSRA LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSRAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IFGNLQQQN MVVHDLQEET+SFLPT+C  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Lag0011456 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 766.9 bits (1979), Expect = 9.7e-218
Identity = 390/459 (84.97%), Postives = 420/459 (91.50%), Query Frame = 0

Query: 1   MTNSLRSIRYPIVMV-VLVTTLFTGTSSALSSTLSRRALQRP-ESPSDGFRVRLNHVDHV 60
           MT SL S R  I++V VL+TTLF  T +     LSRRALQ+  E PS+GF+V+LNHVDHV
Sbjct: 1   MTVSLHSYRCLILLVIVLITTLFIDTLA-----LSRRALQKSNELPSNGFKVKLNHVDHV 60

Query: 61  KNLTRFERLQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPR 120
           KNLTRFERL+RGV RG+NRL RLNAMVLAANAAVG+QV+APVVAGNGEFLMKLAIG+PP+
Sbjct: 61  KNLTRFERLRRGVARGKNRLHRLNAMVLAANAAVGEQVQAPVVAGNGEFLMKLAIGTPPK 120

Query: 121 RFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGD 180
            FSAIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP+ SSSFSK+SCSSELCG LPTSTC  D
Sbjct: 121 SFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPTSTCSSD 180

Query: 181 SCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGL 240
            CEY YTYGD SST GVLA ETFTFGDSSDDQVSI+G+GFGCGDDNEGDGFSQGAGLVGL
Sbjct: 181 GCEYLYTYGDSSSTQGVLALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFSQGAGLVGL 240

Query: 241 GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQP 300
           GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+KPKT+++EMKTTPLIRNPSQP
Sbjct: 241 GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQP 300

Query: 301 SFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQ 360
           SFYYLSLQGISVG TQLS+PK TFEL DDGSGG+IIDSGTTITYIE +AF LLK EFIAQ
Sbjct: 301 SFYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQ 360

Query: 361 MKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLA 420
           M LPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHF+GADLELPGENYMIGDSR GLICLA
Sbjct: 361 MNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRTGLICLA 420

Query: 421 IGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IGSS GMSIFGNLQQQNFMVVHDLQEET+SFLPT+C SI
Sbjct: 421 IGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

BLAST of Lag0011456 vs. NCBI nr
Match: XP_022154910.1 (aspartic proteinase nepenthesin-1 [Momordica charantia])

HSP 1 Score: 766.1 bits (1977), Expect = 1.7e-217
Identity = 387/460 (84.13%), Postives = 421/460 (91.52%), Query Frame = 0

Query: 1   MTNSLRSIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVD-HVK 60
           M +SL S+RYPIV+V ++ TLF    +  SSTLSRRALQ+ +  ++GFR+RL+HVD HVK
Sbjct: 1   MLDSLCSVRYPIVLVAVLATLFFIDLTVSSSTLSRRALQQQKLLNNGFRMRLHHVDHHVK 60

Query: 61  NLTRFERLQRGVMRGRNRLQRLNAMVLAA--NAAVGDQVRAPVVAGNGEFLMKLAIGSPP 120
           NLTRFERLQRG  RGRNRLQRLNAMVLAA   AAVGDQV+APVVAGNGEFLMKLAIGSPP
Sbjct: 61  NLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120

Query: 121 RRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDG 180
           + FSAIMDTGSDLIWTQCKPC+QCFDQSTPIFDP+ESSSFSKLSCSSELCGALPTS C  
Sbjct: 121 KSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSACSN 180

Query: 181 DSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVG 240
           D CEY YTYGD+SSTHG+L AETFTFGD  +DQVSIS +GFGCGDDNEGDGFSQGAGLVG
Sbjct: 181 DGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAGLVG 240

Query: 241 LGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQ 300
           LGRGPLSLVSQLKEQKFAYCLT IDDSKPSSLL+GSLANVKPK SQ+E+KTTPLIRNPSQ
Sbjct: 241 LGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRNPSQ 300

Query: 301 PSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIA 360
           PSFYYLSL+GISVGG+ L++P+ TFELRDDGSGG+IIDSGTTITYI+KSAF LLKKEFIA
Sbjct: 301 PSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKEFIA 360

Query: 361 QMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICL 420
           QMKLPVDDSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL+CL
Sbjct: 361 QMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGLLCL 420

Query: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           AIGSSSGMSIFGNLQQQNFMVVHDLQEETISF+PT+C  I
Sbjct: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

BLAST of Lag0011456 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.0e-116
Identity = 230/453 (50.77%), Postives = 303/453 (66.89%), Query Frame = 0

Query: 5   LRSIRYPIVMVVLVTTLFTGTSSALSSTLSRRAL-QRPESPSDGFRVRLNHVDHVKNLTR 64
           + S  Y  ++ + +  +F     A + + SR AL  R E+   GF++ L HVD  KNLT+
Sbjct: 1   MASSLYSFLLALSIVYIFV----APTHSTSRTALNHRHEAKVTGFQIMLEHVDSGKNLTK 60

Query: 65  FERLQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAI 124
           F+ L+R + RG  RLQRL AM+   N   G  V   V AG+GE+LM L+IG+P + FSAI
Sbjct: 61  FQLLERAIERGSRRLQRLEAML---NGPSG--VETSVYAGDGEYLMNLSIGTPAQPFSAI 120

Query: 125 MDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYF 184
           MDTGSDLIWTQC+PC QCF+QSTPIF+P+ SSSFS L CSS+LC AL + TC  + C+Y 
Sbjct: 121 MDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYT 180

Query: 185 YTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPL 244
           Y YGD S T G +  ET TFG      VSI  + FGCG++N+G G   GAGLVG+GRGPL
Sbjct: 181 YGYGDGSETQGSMGTETLTFG-----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPL 240

Query: 245 SLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYL 304
           SL SQL   KF+YC+T I  S PS+LLLGSLAN     S      T LI++   P+FYY+
Sbjct: 241 SLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS----PNTTLIQSSQIPTFYYI 300

Query: 305 SLQGISVGGTQLSMPKATFELR-DDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLP 364
           +L G+SVG T+L +  + F L  ++G+GG+IIDSGTT+TY   +A+  +++EFI+Q+ LP
Sbjct: 301 TLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLP 360

Query: 365 VDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSS 424
           V +  + G DLCF  P++ + +++P    HF G DLELP ENY I  S  GLICLA+GSS
Sbjct: 361 VVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSN-GLICLAMGSS 420

Query: 425 S-GMSIFGNLQQQNFMVVHDLQEETISFLPTEC 455
           S GMSIFGN+QQQN +VV+D     +SF   +C
Sbjct: 421 SQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Lag0011456 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 4.7e-114
Identity = 213/448 (47.54%), Postives = 302/448 (67.41%), Query Frame = 0

Query: 10  YPIVM-VVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFERLQ 69
           Y +V+ + +V+ +   TSS    TL     +RP+    G RV L  VD  KNLT++E ++
Sbjct: 6   YSVVLGLAIVSAIVAPTSSTSRGTLLHHGQKRPQ---PGLRVDLEQVDSGKNLTKYELIK 65

Query: 70  RGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDTGS 129
           R + RG  R++ +NAM+ +++      +  PV AG+GE+LM +AIG+P   FSAIMDTGS
Sbjct: 66  RAIKRGERRMRSINAMLQSSSG-----IETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGS 125

Query: 130 DLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGD 189
           DLIWTQC+PC QCF Q TPIF+P++SSSFS L C S+ C  LP+ TC+ + C+Y Y YGD
Sbjct: 126 DLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGD 185

Query: 190 FSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQ 249
            S+T G +A ETFTF  S     S+  + FGCG+DN+G G   GAGL+G+G GPLSL SQ
Sbjct: 186 GSTTQGYMATETFTFETS-----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQ 245

Query: 250 LKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGI 309
           L   +F+YC+T+   S PS+L LGS A+  P+ S     +T LI +   P++YY++LQGI
Sbjct: 246 LGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGS----PSTTLIHSSLNPTYYYITLQGI 305

Query: 310 SVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDSGT 369
           +VGG  L +P +TF+L+DDG+GG+IIDSGTT+TY+ + A+N + + F  Q+ LP  D  +
Sbjct: 306 TVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESS 365

Query: 370 GGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSS--GMS 429
            GL  CF  P++ + V+VP+++  F G  L L  +N +I  +  G+ICLA+GSSS  G+S
Sbjct: 366 SGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGIS 425

Query: 430 IFGNLQQQNFMVVHDLQEETISFLPTEC 455
           IFGN+QQQ   V++DLQ   +SF+PT+C
Sbjct: 426 IFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Lag0011456 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 264.2 bits (674), Expect = 2.7e-69
Identity = 165/465 (35.48%), Postives = 242/465 (52.04%), Query Frame = 0

Query: 26  SSALSSTLSRRALQRPESPSDGFRVRLNHVD----HVKNLTRFERLQRGVMRGRNRLQRL 85
           ++ L   L+  AL    +P   FR+ L  VD       NLT  E L+R + R R RL  +
Sbjct: 3   AAVLLLLLALAALPASCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGI 62

Query: 86  N-AMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQ 145
             A   AA+A        P++   GE+L+KL IG+PP +F+A +DT SDLIWTQC+PC  
Sbjct: 63  GMARGEAASARKAVVAETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTG 122

Query: 146 CFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTC---DGDSCEYFYTYGDFSSTHGVLA 205
           C+ Q  P+F+P  SS+++ L CSS+ C  L    C   D +SC+Y YTY   ++T G LA
Sbjct: 123 CYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLA 182

Query: 206 AETFTFGDSSDDQVSISGVGFGCGDDNEGDG-FSQGAGLVGLGRGPLSLVSQLKEQKFAY 265
            +    G+      +  GV FGC   + G     Q +G+VGLGRGPLSLVSQL  ++FAY
Sbjct: 183 VDKLVIGED-----AFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAY 242

Query: 266 CLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLS 325
           CL       P  L+LG+ A+     +       P+ R+P  PS+YYL+L G+ +G   +S
Sbjct: 243 CLPPPASRIPGKLVLGADADAARNATNR--IAVPMRRDPRYPSYYYLNLDGLLIGDRAMS 302

Query: 326 MPKAT-----------------------FELRDDGSGGLIIDSGTTITYIEKSAFNLLKK 385
           +P  T                         + D    G+IID  +TIT++E S ++ L  
Sbjct: 303 LPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVN 362

Query: 386 EFIAQMKLPVDDSGTGGLDLCFNLP--AEATQVEVPKLTFHFKGADLELPGENYMIGDSR 445
           +   +++LP     + GLDLCF LP      +V VP +   F G  L L        D  
Sbjct: 363 DLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRE 422

Query: 446 AGLICLAIG--SSSGMSIFGNLQQQNFMVVHDLQEETISFLPTEC 455
           +G++CL +G   +  +SI GN QQQN  V+++L+   ++F+ + C
Sbjct: 423 SGMMCLMVGRAEAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPC 460

BLAST of Lag0011456 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.0e-68
Identity = 144/368 (39.13%), Postives = 211/368 (57.34%), Query Frame = 0

Query: 94  DQVRAPVVA----GNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIF 153
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ QS P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 154 DPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDD 213
           +P  SS++  L+CS+  C  L TS C  + C Y  +YGD S T G LA +T TFG+S   
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSG-- 264

Query: 214 QVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 273
              I+ V  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 --KINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 274 LLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGS 333
              S+             T PL+RN    +FYY+ L G SVGG ++ +P A F++   GS
Sbjct: 325 DFNSVQLGGGDA------TAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 334 GGLIIDSGTTITYIEKSAFNLLKKEFI-AQMKLPVDDSGTGGLDLCFNLPAEATQVEVPK 393
           GG+I+D GT +T ++  A+N L+  F+   + L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 394 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSSGMSIFGNLQQQNFMVVHDLQEET 453
           + FHF G   L+LP +NY+I    +G  C A   +SS +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 454 ISFLPTEC 455
           I     +C
Sbjct: 505 IGLSGNKC 500

BLAST of Lag0011456 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.1e-66
Identity = 152/423 (35.93%), Postives = 230/423 (54.37%), Query Frame = 0

Query: 44  PSDGFRVRLNHVDHVKNLTRFERLQRGVMRGRNRLQR-LNAMVLAANAAVGDQVRAPVVA 103
           P  GF   L H D  K+   +  ++    R RN + R +N +          Q +  + +
Sbjct: 27  PKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTS 86

Query: 104 GNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSC 163
            +GE+LM ++IG+PP    AI DTGSDL+WTQC PC+ C+ Q  P+FDP+ SS++  +SC
Sbjct: 87  NSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSC 146

Query: 164 SSELCGAL---PTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFG 223
           SS  C AL    + + + ++C Y  +YGD S T G +A +T T G S    + +  +  G
Sbjct: 147 SSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIG 206

Query: 224 CGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ---KFAYCLTAIDDSK--PSSLLLGSL 283
           CG +N G    +G+G+VGLG GP+SL+ QL +    KF+YCL  +   K   S +  G+ 
Sbjct: 207 CGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTN 266

Query: 284 ANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLII 343
           A V    S   + +TPLI   SQ +FYYL+L+ ISVG  Q+    +  E      G +II
Sbjct: 267 AIV----SGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSE---SSEGNIII 326

Query: 344 DSGTTITYIEKSAFNLLKKEFIAQMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFK 403
           DSGTT+T +    ++ L+    + +          GL LC++   +   ++VP +T HF 
Sbjct: 327 DSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSATGD---LKVPVITMHFD 386

Query: 404 GADLELPGENYMIGDSRAGLICLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTEC 458
           GAD++L   N  +  S   L+C A   S   SI+GN+ Q NF+V +D   +T+SF PT+C
Sbjct: 387 GADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 437

BLAST of Lag0011456 vs. ExPASy TrEMBL
Match: A0A6J1EPU6 (aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 9.4e-219
Identity = 384/451 (85.14%), Postives = 413/451 (91.57%), Query Frame = 0

Query: 7   SIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFER 66
           S+RY I+ VVL  T+    +SA SS+LSRRAL +P+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDT 126
           LQRGV RG+ RL RLNAMVLAAN  VG +V+APVVAGNGEFLMKLAIGSPPR FSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPC+QCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLL+GSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPVDDS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IFGNLQQQN MVVHDLQEET+SFLPT+C  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

BLAST of Lag0011456 vs. ExPASy TrEMBL
Match: A0A6J1DKZ2 (aspartic proteinase nepenthesin-1 OS=Momordica charantia OX=3673 GN=LOC111022058 PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 8.0e-218
Identity = 387/460 (84.13%), Postives = 421/460 (91.52%), Query Frame = 0

Query: 1   MTNSLRSIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVD-HVK 60
           M +SL S+RYPIV+V ++ TLF    +  SSTLSRRALQ+ +  ++GFR+RL+HVD HVK
Sbjct: 1   MLDSLCSVRYPIVLVAVLATLFFIDLTVSSSTLSRRALQQQKLLNNGFRMRLHHVDHHVK 60

Query: 61  NLTRFERLQRGVMRGRNRLQRLNAMVLAA--NAAVGDQVRAPVVAGNGEFLMKLAIGSPP 120
           NLTRFERLQRG  RGRNRLQRLNAMVLAA   AAVGDQV+APVVAGNGEFLMKLAIGSPP
Sbjct: 61  NLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120

Query: 121 RRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDG 180
           + FSAIMDTGSDLIWTQCKPC+QCFDQSTPIFDP+ESSSFSKLSCSSELCGALPTS C  
Sbjct: 121 KSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSACSN 180

Query: 181 DSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVG 240
           D CEY YTYGD+SSTHG+L AETFTFGD  +DQVSIS +GFGCGDDNEGDGFSQGAGLVG
Sbjct: 181 DGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAGLVG 240

Query: 241 LGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQ 300
           LGRGPLSLVSQLKEQKFAYCLT IDDSKPSSLL+GSLANVKPK SQ+E+KTTPLIRNPSQ
Sbjct: 241 LGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRNPSQ 300

Query: 301 PSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIA 360
           PSFYYLSL+GISVGG+ L++P+ TFELRDDGSGG+IIDSGTTITYI+KSAF LLKKEFIA
Sbjct: 301 PSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKEFIA 360

Query: 361 QMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICL 420
           QMKLPVDDSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL+CL
Sbjct: 361 QMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGLLCL 420

Query: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           AIGSSSGMSIFGNLQQQNFMVVHDLQEETISF+PT+C  I
Sbjct: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

BLAST of Lag0011456 vs. ExPASy TrEMBL
Match: A0A6J1HXS0 (aspartic proteinase nepenthesin-1 OS=Cucurbita maxima OX=3661 GN=LOC111467205 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.4e-216
Identity = 380/451 (84.26%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 7   SIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNLTRFER 66
           S+RY IV VVL  T+    +SA SS+LSRRAL++P+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIVSVVLSITMLFIHTSASSSSLSRRALRQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +V+APVVAGNGEFLMKLAIGSPPR FSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANIGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPC+QCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S  E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASDGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDS 366
           GISVGGTQL +PK TFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPVDDS
Sbjct: 304 GISVGGTQLPIPKNTFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP +  QVEVPKLTFHFKGADLELPGENYMIGDS+A LICL IGSS+GMS
Sbjct: 364 GTSGLDLCFNLPPKTNQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLTIGSSNGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           IFGNLQQQN MVVHDLQEET+SFLPT+C  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Lag0011456 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.8e-216
Identity = 384/458 (83.84%), Postives = 416/458 (90.83%), Query Frame = 0

Query: 4   SLRSIRY--PIVMVVLVTTLFTGTSSALSSTLSRRALQRPES-PSDGFRVRLNHVDHVKN 63
           SLRS  Y   +++++L+TTLF  T  A SS+LSRRALQ+P   PS GFRVRL HVDHVKN
Sbjct: 5   SLRSFGYLHRLLLIILITTLFINT-LAFSSSLSRRALQKPNKLPSHGFRVRLKHVDHVKN 64

Query: 64  LTRFERLQRGVMRGRNRLQRLNAMVL-AANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRR 123
           LTRFERL+RGV RG+NRL RLNAMVL AANA VGDQV+APVVAGNGEFLMKLAIGSPPR 
Sbjct: 65  LTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRS 124

Query: 124 FSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDS 183
           FSAIMDTGSDLIWTQCKPC+QCFDQSTPIFDP++SSSF K+SCSSELCGALPTSTC  D 
Sbjct: 125 FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCSSDG 184

Query: 184 CEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLG 243
           CEY YTYGD SST GVLA ETFTFGDS++DQ+SI G+GFGCG+DN GDGFSQGAGLVGLG
Sbjct: 185 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 244

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+ PKTS++EMKTTPLI+NPSQPS
Sbjct: 245 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPS 304

Query: 304 FYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQM 363
           FYYLSLQGISVGGTQLS+PK+TFEL DDGSGG+IIDSGTTITY+E SAF  LK EFIAQM
Sbjct: 305 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFIAQM 364

Query: 364 KLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAI 423
            LPVDDSGTGGLDLCFNLPA   QVEVPKLTFHFKGADLELPGENYMIGDS+AGL+CLAI
Sbjct: 365 NLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAI 424

Query: 424 GSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           GSS GMSIFGNLQQQNFMVVHDLQEET+SFLPT+C SI
Sbjct: 425 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

BLAST of Lag0011456 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 7.0e-214
Identity = 381/458 (83.19%), Postives = 415/458 (90.61%), Query Frame = 0

Query: 4   SLRSIRY--PIVMVVLVTTLFTGTSSALSSTLSRRALQRPES-PSDGFRVRLNHVDHVKN 63
           SLRS  Y   ++++V +TTLF  T  A SS+LS RALQ+P   PS GFRVRL HVDHVKN
Sbjct: 5   SLRSFGYLQLLLLIVFITTLFINT-LAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKN 64

Query: 64  LTRFERLQRGVMRGRNRLQRLNAMVL-AANAAVGDQVRAPVVAGNGEFLMKLAIGSPPRR 123
           LTRFERL+RGV RG+NRL RLNAMVL AANA+VGDQV+APVVAGNGEFLMKLAIGSPPR 
Sbjct: 65  LTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRS 124

Query: 124 FSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDS 183
           FSAIMDTGSDLIWTQCKPC+QCFDQ+TPIFDP++SSSFSK+SC SELCGALPTSTC  D 
Sbjct: 125 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDG 184

Query: 184 CEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLG 243
           CEY YTYGD SST GVLA ETFTFGDS++DQ+SI G+GFGCG+DN GDGFSQGAGLVGLG
Sbjct: 185 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 244

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+ PKTS++EMK TPLI+NPSQPS
Sbjct: 245 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPS 304

Query: 304 FYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQM 363
           FYYLSLQGISVGGTQLS+PK+TFEL DDGSGG+IIDSGTTITYIE +AF+ LK EFIAQM
Sbjct: 305 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQM 364

Query: 364 KLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAI 423
            LPVDDSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAI
Sbjct: 365 NLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAI 424

Query: 424 GSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTECVSI 458
           GSS GMSIFGNLQQQNFMVVHDLQEET+SFLPT+C SI
Sbjct: 425 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

BLAST of Lag0011456 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.5e-155
Identity = 283/464 (60.99%), Postives = 361/464 (77.80%), Query Frame = 0

Query: 2   TNSLRSIRYPIVMVVLVTTLFTGTSSALSSTLSRRALQRPESPSDGFRVRLNHVDHVKNL 61
           ++S  S+ +P   ++L + L + +SS  S  L  R L +   P  GFR+ L HVD  KNL
Sbjct: 3   SSSSSSLLFPF-FLILFSCLISVSSSRRS--LIDRTLPK-NLPRSGFRLSLRHVDSGKNL 62

Query: 62  TRFERLQRGVMRGRNRLQRLNAMVLAANAAVGD---QVRAPVVAGNGEFLMKLAIGSPPR 121
           T+ +++QRG+ RG +RL RL A+ + A A+  D    ++AP   G+GEFLM+L+IG+P  
Sbjct: 63  TKIQKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAV 122

Query: 122 RFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTC--D 181
           ++SAI+DTGSDLIWTQCKPC +CFDQ TPIFDPE+SSS+SK+ CSS LC ALP S C  D
Sbjct: 123 KYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNED 182

Query: 182 GDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLV 241
            D+CEY YTYGD+SST G+LA ETFTF    +D+ SISG+GFGCG +NEGDGFSQG+GLV
Sbjct: 183 KDACEYLYTYGDYSSTRGLLATETFTF----EDENSISGIGFGCGVENEGDGFSQGSGLV 242

Query: 242 GLGRGPLSLVSQLKEQKFAYCLTAIDDSK-PSSLLLGSLAN-VKPKTSQ----EEMKTTP 301
           GLGRGPLSL+SQLKE KF+YCLT+I+DS+  SSL +GSLA+ +  KT      E  KT  
Sbjct: 243 GLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMS 302

Query: 302 LIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNL 361
           L+RNP QPSFYYL LQGI+VG  +LS+ K+TFEL +DG+GG+IIDSGTTITY+E++AF +
Sbjct: 303 LLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKV 362

Query: 362 LKKEFIAQMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDS 421
           LK+EF ++M LPVDDSG+ GLDLCF LP  A  + VPK+ FHFKGADLELPGENYM+ DS
Sbjct: 363 LKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADS 422

Query: 422 RAGLICLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTEC 455
             G++CLA+GSS+GMSIFGN+QQQNF V+HDL++ET+SF+PTEC
Sbjct: 423 STGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTEC 458

BLAST of Lag0011456 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 262.3 bits (669), Expect = 7.2e-70
Identity = 144/368 (39.13%), Postives = 211/368 (57.34%), Query Frame = 0

Query: 94  DQVRAPVVA----GNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIF 153
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ QS P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 154 DPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDD 213
           +P  SS++  L+CS+  C  L TS C  + C Y  +YGD S T G LA +T TFG+S   
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSG-- 264

Query: 214 QVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 273
              I+ V  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 --KINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 274 LLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGS 333
              S+             T PL+RN    +FYY+ L G SVGG ++ +P A F++   GS
Sbjct: 325 DFNSVQLGGGDA------TAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 334 GGLIIDSGTTITYIEKSAFNLLKKEFI-AQMKLPVDDSGTGGLDLCFNLPAEATQVEVPK 393
           GG+I+D GT +T ++  A+N L+  F+   + L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 394 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSSGMSIFGNLQQQNFMVVHDLQEET 453
           + FHF G   L+LP +NY+I    +G  C A   +SS +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 454 ISFLPTEC 455
           I     +C
Sbjct: 505 IGLSGNKC 500

BLAST of Lag0011456 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-69
Identity = 161/425 (37.88%), Postives = 224/425 (52.71%), Query Frame = 0

Query: 49  RVRLNHVDH--VKNLTRFERLQRGVMRGRNRLQRLNAMVLAANAA-----------VGDQ 108
           RV +   +H   K+LT   RL R   R ++ + RL+  +   + A               
Sbjct: 74  RVSVRGTEHSDYKSLT-LARLNRDTARVKSLITRLDLAINNISKADLKPISTMYTTEEQD 133

Query: 109 VRAPVVA----GNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDP 168
           + AP+++    G+GE+  ++ IG P R    ++DTGSD+ W QC PC  C+ Q+ PIF+P
Sbjct: 134 IEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEP 193

Query: 169 EESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQV 228
             SSS+  LSC +  C AL  S C   +C Y  +YGD S T G  A ET T G +     
Sbjct: 194 SSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGST----- 253

Query: 229 SISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLL 288
            +  V  GCG  NEG  F   AGL+GLG G L+L SQL    F+YCL   D    S++  
Sbjct: 254 LVQNVAVGCGHSNEG-LFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSASTVDF 313

Query: 289 GSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGSGG 348
           G+       +   +    PL+RN    +FYYL L GISVGG  L +P+++FE+ + GSGG
Sbjct: 314 GT-------SLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGG 373

Query: 349 LIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTF 408
           +IIDSGT +T ++   +N L+  F+         +G    D C+NL A+ T VEVP + F
Sbjct: 374 IIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTT-VEVPTVAF 433

Query: 409 HFKGAD-LELPGENYMIGDSRAGLICLAIG-SSSGMSIFGNLQQQNFMVVHDLQEETISF 455
           HF G   L LP +NYMI     G  CLA   ++S ++I GN+QQQ   V  DL    I F
Sbjct: 434 HFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSLIGF 483

BLAST of Lag0011456 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 252.7 bits (644), Expect = 5.7e-67
Identity = 152/423 (35.93%), Postives = 230/423 (54.37%), Query Frame = 0

Query: 44  PSDGFRVRLNHVDHVKNLTRFERLQRGVMRGRNRLQR-LNAMVLAANAAVGDQVRAPVVA 103
           P  GF   L H D  K+   +  ++    R RN + R +N +          Q +  + +
Sbjct: 27  PKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNAIHRSVNRVFHFTEKDNTPQPQIDLTS 86

Query: 104 GNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPEESSSFSKLSC 163
            +GE+LM ++IG+PP    AI DTGSDL+WTQC PC+ C+ Q  P+FDP+ SS++  +SC
Sbjct: 87  NSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSC 146

Query: 164 SSELCGAL---PTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFG 223
           SS  C AL    + + + ++C Y  +YGD S T G +A +T T G S    + +  +  G
Sbjct: 147 SSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIG 206

Query: 224 CGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ---KFAYCLTAIDDSK--PSSLLLGSL 283
           CG +N G    +G+G+VGLG GP+SL+ QL +    KF+YCL  +   K   S +  G+ 
Sbjct: 207 CGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVPLTSKKDQTSKINFGTN 266

Query: 284 ANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLII 343
           A V    S   + +TPLI   SQ +FYYL+L+ ISVG  Q+    +  E      G +II
Sbjct: 267 AIV----SGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSE---SSEGNIII 326

Query: 344 DSGTTITYIEKSAFNLLKKEFIAQMKLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFK 403
           DSGTT+T +    ++ L+    + +          GL LC++   +   ++VP +T HF 
Sbjct: 327 DSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSATGD---LKVPVITMHFD 386

Query: 404 GADLELPGENYMIGDSRAGLICLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTEC 458
           GAD++L   N  +  S   L+C A   S   SI+GN+ Q NF+V +D   +T+SF PT+C
Sbjct: 387 GADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 437

BLAST of Lag0011456 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 252.3 bits (643), Expect = 7.5e-67
Identity = 161/430 (37.44%), Postives = 232/430 (53.95%), Query Frame = 0

Query: 45  SDGFRVRLNHVDHV-KNLTRFE----RLQRGVMRGRNRLQRLNAMVLAAN-------AAV 104
           S    + L+H+D +  N T  E    RLQR   R ++ +  L A +   N          
Sbjct: 69  SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS-IATLAAQIPGRNVTHAPRPGGF 128

Query: 105 GDQVRAPVVAGNGEFLMKLAIGSPPRRFSAIMDTGSDLIWTQCKPCEQCFDQSTPIFDPE 164
              V + +  G+GE+  +L +G+P R    ++DTGSD++W QC PC +C+ QS PIFDP 
Sbjct: 129 SSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPR 188

Query: 165 ESSSFSKLSCSSELCGALPTSTCD--GDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQ 224
           +S +++ + CSS  C  L ++ C+    +C Y  +YGD S T G  + ET TF  +    
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRN---- 248

Query: 225 VSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLK---EQKFAYCLT-AIDDSKP 284
             + GV  GCG DNEG  F   AGL+GLG+G LS   Q      QKF+YCL      SKP
Sbjct: 249 -RVKGVALGCGHDNEG-LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 308

Query: 285 SSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQL-SMPKATFELR 344
           SS++ G+ A           + TPL+ NP   +FYY+ L GISVGGT++  +  + F+L 
Sbjct: 309 SSVVFGNAA------VSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLD 368

Query: 345 DDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVDDSGTGGLDLCFNLPAEATQVE 404
             G+GG+IIDSGT++T + + A+  ++  F    K           D CF+L +   +V+
Sbjct: 369 QIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVK 428

Query: 405 VPKLTFHFKGADLELPGENYMIGDSRAGLICLAI-GSSSGMSIFGNLQQQNFMVVHDLQE 455
           VP +  HF+GAD+ LP  NY+I     G  C A  G+  G+SI GN+QQQ F VV+DL  
Sbjct: 429 VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLAS 484