Lag0011429 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011429
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUPF0481 protein At3g47200-like
Locationchr1: 24569900 .. 24571162 (-)
RNA-Seq ExpressionLag0011429
SyntenyLag0011429
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGCATCTTCCATAAAACAAATGCTTCAGCAACTACCTCCTATCGCCGAAGAATGTAGCATCTATAGAGTTCCTACACGGTTACGCAACATTAATCATATCGCCTATACTCCTCAAGTCATTTCCATTGGCCCTTTCCACCATTGGCCAAAGGATTTGATGGCCACCGAAGAGCTTAAACTTCGATGTTTGGATAGTTACCTACGTCGTGATATAAAATTGAAAGTTGAGGACGCCGTGAAGAATGCCAAGAGTTGGGAGAGTAAAGCCCGTCGTTTCTATGCAGAACCTATAACCATGAATAGCGACGACTTTGTCAAAATGATGCTTGTAGATGGATGTTTCATAGTGGAGGTCATGATAATATATTACGGTGGATGTTTCGAAACTCAAAACGGGTTAGATCCTTCATTCTTCAAAGCTCTAAGAATCGACTTATATCGTGACTTGACAATGCTCGAGAATCAACTCCCCTTCTTTGTTCTTGAACAACTATTTAACATGCTTCCTCAAGATGGAGAGAAAAATAATAATCGTGTCTCGTTTATACAACTTACCCACAAATTTTTCAGTATTGGGTTGATAAAAGATAACTATGAGCTTCCTCATGATGTCTCCACACAAAAAATAAACCACTTGCTCGATTTCTTAAGGTTCTACTACGTCCCATCGAAATCTGTGAAGGAAAGTGGAGAAAAGAGGGAGTTTCTGCTTCCTCCCACTATAACTGAGCTTTGTGAGGCTGGTGTCATCGTTAAAAAGGCAACAAAAGGTAAACGCTTGATGGACATAAGCTTCCAAGATGGGGTCCTAGAAATCCCACATTTTGAAGTTCATGATCATTTTGAAACCTATGTGCGAAACCTAATTGCATTTGAGCATTACCCTGTGGGGAATGATGAGAGGTATGTAATCCATTATGTTGAGTTCTTAGATGGCTTGATCAGCACGGAGAAAGATGCAAGTTTACTTGTGAAGGCGAAAATCATAACCAACCATATTGGTGGCAGTGACGAAGAAATTTCGAAACTTTTCAACAATCTTTGTAAAGATACCACCATTCCAAGTGATTTTTACTACTATGCTACGAGCAAAGCTTTACATGATCACTGCTTGACACGACGACACCGGTGGATGGCTTCATTAAGACGAGATTATTTCAATACACCATGGGCTTCTATCTCTTTCACCGCCGCTACCTTCCTCATTTTACTCACTTTCCTTCAAACCTTATCATCTTTTCTATCACTTTCAAAGTAA

mRNA sequence

ATGCTTGCATCTTCCATAAAACAAATGCTTCAGCAACTACCTCCTATCGCCGAAGAATGTAGCATCTATAGAGTTCCTACACGGTTACGCAACATTAATCATATCGCCTATACTCCTCAAGTCATTTCCATTGGCCCTTTCCACCATTGGCCAAAGGATTTGATGGCCACCGAAGAGCTTAAACTTCGATGTTTGGATAGTTACCTACGTCGTGATATAAAATTGAAAGTTGAGGACGCCGTGAAGAATGCCAAGAGTTGGGAGAGTAAAGCCCGTCGTTTCTATGCAGAACCTATAACCATGAATAGCGACGACTTTGTCAAAATGATGCTTGTAGATGGATGTTTCATAGTGGAGGTCATGATAATATATTACGGTGGATGTTTCGAAACTCAAAACGGGTTAGATCCTTCATTCTTCAAAGCTCTAAGAATCGACTTATATCGTGACTTGACAATGCTCGAGAATCAACTCCCCTTCTTTGTTCTTGAACAACTATTTAACATGCTTCCTCAAGATGGAGAGAAAAATAATAATCGTGTCTCGTTTATACAACTTACCCACAAATTTTTCAGTATTGGGTTGATAAAAGATAACTATGAGCTTCCTCATGATGTCTCCACACAAAAAATAAACCACTTGCTCGATTTCTTAAGGTTCTACTACGTCCCATCGAAATCTGTGAAGGAAAGTGGAGAAAAGAGGGAGTTTCTGCTTCCTCCCACTATAACTGAGCTTTGTGAGGCTGGTGTCATCGTTAAAAAGGCAACAAAAGGTAAACGCTTGATGGACATAAGCTTCCAAGATGGGGTCCTAGAAATCCCACATTTTGAAGTTCATGATCATTTTGAAACCTATGTGCGAAACCTAATTGCATTTGAGCATTACCCTGTGGGGAATGATGAGAGGTATGTAATCCATTATGTTGAGTTCTTAGATGGCTTGATCAGCACGGAGAAAGATGCAAGTTTACTTGTGAAGGCGAAAATCATAACCAACCATATTGGTGGCAGTGACGAAGAAATTTCGAAACTTTTCAACAATCTTTGTAAAGATACCACCATTCCAAGTGATTTTTACTACTATGCTACGAGCAAAGCTTTACATGATCACTGCTTGACACGACGACACCGGTGGATGGCTTCATTAAGACGAGATTATTTCAATACACCATGGGCTTCTATCTCTTTCACCGCCGCTACCTTCCTCATTTTACTCACTTTCCTTCAAACCTTATCATCTTTTCTATCACTTTCAAAGTAA

Coding sequence (CDS)

ATGCTTGCATCTTCCATAAAACAAATGCTTCAGCAACTACCTCCTATCGCCGAAGAATGTAGCATCTATAGAGTTCCTACACGGTTACGCAACATTAATCATATCGCCTATACTCCTCAAGTCATTTCCATTGGCCCTTTCCACCATTGGCCAAAGGATTTGATGGCCACCGAAGAGCTTAAACTTCGATGTTTGGATAGTTACCTACGTCGTGATATAAAATTGAAAGTTGAGGACGCCGTGAAGAATGCCAAGAGTTGGGAGAGTAAAGCCCGTCGTTTCTATGCAGAACCTATAACCATGAATAGCGACGACTTTGTCAAAATGATGCTTGTAGATGGATGTTTCATAGTGGAGGTCATGATAATATATTACGGTGGATGTTTCGAAACTCAAAACGGGTTAGATCCTTCATTCTTCAAAGCTCTAAGAATCGACTTATATCGTGACTTGACAATGCTCGAGAATCAACTCCCCTTCTTTGTTCTTGAACAACTATTTAACATGCTTCCTCAAGATGGAGAGAAAAATAATAATCGTGTCTCGTTTATACAACTTACCCACAAATTTTTCAGTATTGGGTTGATAAAAGATAACTATGAGCTTCCTCATGATGTCTCCACACAAAAAATAAACCACTTGCTCGATTTCTTAAGGTTCTACTACGTCCCATCGAAATCTGTGAAGGAAAGTGGAGAAAAGAGGGAGTTTCTGCTTCCTCCCACTATAACTGAGCTTTGTGAGGCTGGTGTCATCGTTAAAAAGGCAACAAAAGGTAAACGCTTGATGGACATAAGCTTCCAAGATGGGGTCCTAGAAATCCCACATTTTGAAGTTCATGATCATTTTGAAACCTATGTGCGAAACCTAATTGCATTTGAGCATTACCCTGTGGGGAATGATGAGAGGTATGTAATCCATTATGTTGAGTTCTTAGATGGCTTGATCAGCACGGAGAAAGATGCAAGTTTACTTGTGAAGGCGAAAATCATAACCAACCATATTGGTGGCAGTGACGAAGAAATTTCGAAACTTTTCAACAATCTTTGTAAAGATACCACCATTCCAAGTGATTTTTACTACTATGCTACGAGCAAAGCTTTACATGATCACTGCTTGACACGACGACACCGGTGGATGGCTTCATTAAGACGAGATTATTTCAATACACCATGGGCTTCTATCTCTTTCACCGCCGCTACCTTCCTCATTTTACTCACTTTCCTTCAAACCTTATCATCTTTTCTATCACTTTCAAAGTAA

Protein sequence

MLASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFIQLTHKFFSIGLIKDNYELPHDVSTQKINHLLDFLRFYYVPSKSVKESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTTIPSDFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTLSSFLSLSK
Homology
BLAST of Lag0011429 vs. NCBI nr
Match: XP_022132118.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 466.5 bits (1199), Expect = 2.5e-127
Identity = 252/420 (60.00%), Postives = 308/420 (73.33%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPI-AEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEEL 61
           L  SI ++L++ PPI A E  IYRVP RL ++   AYTP+VI+IGPFHH   DL+AT++ 
Sbjct: 50  LELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQS 109

Query: 62  KLRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEV 121
           KL C  +YL R I+  V+  V  A+ WE KARR YAEPI M SDDFV+MML+D CFIVE+
Sbjct: 110 KLHCFRNYLIR-IERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLIDACFIVEL 169

Query: 122 MIIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNR 181
           MII    CFET+ G D  F K +  DLY++LTMLENQLPFFVL+ LF++ P    KN   
Sbjct: 170 MIILNFECFETREGFDLLFAKVILHDLYQELTMLENQLPFFVLQALFDLFPP--HKNKAN 229

Query: 182 VSFIQLTHKFFSIGLIKDNYELPHDV-STQKINHLLDFLRFYYVPSKSVKESGEKRE--- 241
           +SFIQLT KF S GLI+  Y LP  V ST+++NHL+D L FYYVPS   +E  + +E   
Sbjct: 230 ISFIQLTGKFLSNGLIR-GYNLPCGVSSTEEVNHLVDLLGFYYVPSPDTEEYRQNKEIKN 289

Query: 242 --FLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFE 301
             FLLPPTIT+LCEAGV VKKA   + L+DISF+ GVL+IP FE+HD FE YVRNL+AFE
Sbjct: 290 RIFLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDDFEIYVRNLMAFE 349

Query: 302 HYPV--GNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKD 361
            Y V   +D+RYVIHY+EFLDGLIST +D +LLVK  II NHIGGS++E+S+LFNNLCK+
Sbjct: 350 QYCVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNKEVSELFNNLCKN 409

Query: 362 TTIPSDFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTL 413
           T IP  FY+YATSK LHDHC     R  A+LRRDYF++PWA IS  AATFLILL  LQT+
Sbjct: 410 TPIPFQFYFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFAATFLILLALLQTI 465

BLAST of Lag0011429 vs. NCBI nr
Match: XP_022132066.1 (UPF0481 protein At3g47200-like [Momordica charantia])

HSP 1 Score: 438.0 bits (1125), Expect = 9.5e-119
Identity = 244/427 (57.14%), Postives = 303/427 (70.96%), Query Frame = 0

Query: 5   SIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRC 64
           S+K ML++L PI+EECSIYRV  RL NIN +AYTPQ ISIGPFHH  K+ MA E+LKLR 
Sbjct: 28  SMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRF 87

Query: 65  LDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIY 124
           LD+YLRR + + +EDA + A+ WE++AR+ YAE I M SD+FVKMMLVDG F+VE + ++
Sbjct: 88  LDAYLRR-VGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMH 147

Query: 125 YGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFI 184
           Y     TQ  L+ + F+A+ +D+YRDL +LENQLPFF+LE L        +K ++   F+
Sbjct: 148 YQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLL-------DKCSSSTPFV 207

Query: 185 QLTHKFF-----SIGLIKDNYELPHDVSTQKINHLLDFLRFYY----VPSKSVKESGEKR 244
             T  F      +  LI D       + T+K NHL+DFL FYY    V  K+ K    KR
Sbjct: 208 LFTSTFCRWYTGARELISDK------LLTKKPNHLVDFLSFYYALPTVTGKNDKLKYNKR 267

Query: 245 EFLLPPTITELCEAGVIVKKATKGKRL-MDISFQDGVLEIPHFEVHDHFETYVRNLIAFE 304
           E   PPT TEL EAGV  +KAT+ KRL MDI F+DGVL IPH E+HD FETYVRNL+A+E
Sbjct: 268 E--SPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYE 327

Query: 305 HYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTT 364
           HY +G+DER +I YV FLD LISTE+D SLLVKA IITN+IGG++E++SKLFN+LCKD  
Sbjct: 328 HYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDIN 387

Query: 365 IPSDFYYYA-TSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTLS 421
           I  DFYYYA  S  LH +C T  HR MASLRRDYFNTPWA ISF AATFL+LLT +Q + 
Sbjct: 388 ISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIY 438

BLAST of Lag0011429 vs. NCBI nr
Match: XP_022158990.1 (UPF0481 protein At3g47200-like isoform X2 [Momordica charantia])

HSP 1 Score: 435.3 bits (1118), Expect = 6.1e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 31  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 90

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 91  LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 150

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 151 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 210

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 211 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 270

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 271 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 330

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 331 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 390

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 391 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 450

BLAST of Lag0011429 vs. NCBI nr
Match: XP_022158992.1 (UPF0481 protein At3g47200-like isoform X3 [Momordica charantia])

HSP 1 Score: 435.3 bits (1118), Expect = 6.1e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 9   VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 68

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 69  LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 128

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 129 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 188

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 189 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 248

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 249 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 308

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 309 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 368

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 369 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 428

BLAST of Lag0011429 vs. NCBI nr
Match: XP_022158989.1 (UPF0481 protein At3g47200-like isoform X1 [Momordica charantia])

HSP 1 Score: 435.3 bits (1118), Expect = 6.1e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 54  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 113

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 114 LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 173

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 174 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 233

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 234 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 293

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 294 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 353

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 354 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 413

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 414 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 473

BLAST of Lag0011429 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.1e-36
Identity = 127/426 (29.81%), Postives = 199/426 (46.71%), Query Frame = 0

Query: 18  EECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRRDIKLKV 77
           E C I+RVP     +N  AY P+V+SIGP+H+  K L   ++ K R L  +L    K  V
Sbjct: 44  ESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDV 103

Query: 78  ED--AVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFETQNGL 137
           E+   VK     E K R+ Y+E +     D + MM++DGCFI+ V +I  G    ++   
Sbjct: 104 EENVLVKAVVDLEDKIRKSYSEEL-KTGHDLMFMMVLDGCFILMVFLIMSGNIELSE--- 163

Query: 138 DPSF-FKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFIQLTHKFFSIG 197
           DP F    L   +  DL +LENQ+PFFVL+ L+         + NR++F      FF   
Sbjct: 164 DPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLYVGSKIGVSSDLNRIAF-----HFFKNP 223

Query: 198 LIKD-NYELPHDVSTQKINHLLDFLRFYYVP--SKSVKESGEKREFLL----PPTITELC 257
           + K+ +Y   H     K  HLLD +R  ++P  S+S K S    +  L       +  + 
Sbjct: 224 IDKEGSYWEKH--RNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVPSVD 283

Query: 258 EAGVIVKKATKGKRLMDISF------QDGV---------LEIPHFEVHDHFETYVRNLIA 317
              V +  + K  RL  I F      +D +         L+IP         ++  N +A
Sbjct: 284 SKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNKLQIPQLRFDGFISSFFLNCVA 343

Query: 318 FEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKD 377
           FE +   +    +  Y+ F+  L++ E+D + L   K+I  +  GS+ E+S+ F  + KD
Sbjct: 344 FEQFYTDSSNE-ITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKD 403

Query: 378 TTIPSD-FYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQT 418
                D  Y     K ++++     +   A  R  +F +PW  +S  A  F+ILLT LQ+
Sbjct: 404 VVFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSCAVLFVILLTMLQS 457

BLAST of Lag0011429 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 2.3e-14
Identity = 63/217 (29.03%), Postives = 98/217 (45.16%), Query Frame = 0

Query: 21  SIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRRDIKLKVEDA 80
           SI+ VP  L   +  +YTP  +SIGP+H    +L   E  KL        +    +  D 
Sbjct: 44  SIFNVPKALMCSHPDSYTPHRVSIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDL 103

Query: 81  VKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFETQNGLDPSFF 140
           V+  +S E K R  Y + I  N +  + +M VD  F++E + IY     ET         
Sbjct: 104 VEKLQSMEIKIRACYHKYIGFNGETLLWIMAVDSSFLIEFLKIYSFRKVET------LIN 163

Query: 141 KALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNN-RVSFIQLTHKFFSIGLIKDN 200
           +    ++ RD+ M+ENQ+P FVL +      +  E  ++  +S +    K  S  +IK +
Sbjct: 164 RVGHNEILRDIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKFD 223

Query: 201 YELPHDVSTQKINHLLDFLRFYYVPSKSVKESGEKRE 237
            +       Q+ NH+LDFL    VP    +E  E  E
Sbjct: 224 DDQILKAQFQECNHILDFLYQMIVPRIEEEELEEDDE 254

BLAST of Lag0011429 vs. ExPASy TrEMBL
Match: A0A6J1BVD4 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.2e-127
Identity = 252/420 (60.00%), Postives = 308/420 (73.33%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPI-AEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEEL 61
           L  SI ++L++ PPI A E  IYRVP RL ++   AYTP+VI+IGPFHH   DL+AT++ 
Sbjct: 50  LELSIIKLLEEQPPIAAAEWCIYRVPKRLFDMKSTAYTPKVIAIGPFHHGRNDLLATQQS 109

Query: 62  KLRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEV 121
           KL C  +YL R I+  V+  V  A+ WE KARR YAEPI M SDDFV+MML+D CFIVE+
Sbjct: 110 KLHCFRNYLIR-IERDVKYVVAIARQWEIKARRLYAEPINMTSDDFVRMMLIDACFIVEL 169

Query: 122 MIIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNR 181
           MII    CFET+ G D  F K +  DLY++LTMLENQLPFFVL+ LF++ P    KN   
Sbjct: 170 MIILNFECFETREGFDLLFAKVILHDLYQELTMLENQLPFFVLQALFDLFPP--HKNKAN 229

Query: 182 VSFIQLTHKFFSIGLIKDNYELPHDV-STQKINHLLDFLRFYYVPSKSVKESGEKRE--- 241
           +SFIQLT KF S GLI+  Y LP  V ST+++NHL+D L FYYVPS   +E  + +E   
Sbjct: 230 ISFIQLTGKFLSNGLIR-GYNLPCGVSSTEEVNHLVDLLGFYYVPSPDTEEYRQNKEIKN 289

Query: 242 --FLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFE 301
             FLLPPTIT+LCEAGV VKKA   + L+DISF+ GVL+IP FE+HD FE YVRNL+AFE
Sbjct: 290 RIFLLPPTITKLCEAGVKVKKAIGARSLLDISFKRGVLQIPPFEIHDDFEIYVRNLMAFE 349

Query: 302 HYPV--GNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKD 361
            Y V   +D+RYVIHY+EFLDGLIST +D +LLVK  II NHIGGS++E+S+LFNNLCK+
Sbjct: 350 QYCVREPDDDRYVIHYIEFLDGLISTAEDVALLVKEGIIINHIGGSNKEVSELFNNLCKN 409

Query: 362 TTIPSDFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTL 413
           T IP  FY+YATSK LHDHC     R  A+LRRDYF++PWA IS  AATFLILL  LQT+
Sbjct: 410 TPIPFQFYFYATSKNLHDHCRKWWPRSKATLRRDYFHSPWAYISVFAATFLILLALLQTI 465

BLAST of Lag0011429 vs. ExPASy TrEMBL
Match: A0A6J1BR71 (UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE=4 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 4.6e-119
Identity = 244/427 (57.14%), Postives = 303/427 (70.96%), Query Frame = 0

Query: 5   SIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRC 64
           S+K ML++L PI+EECSIYRV  RL NIN +AYTPQ ISIGPFHH  K+ MA E+LKLR 
Sbjct: 28  SMKNMLEKLHPISEECSIYRVSKRLHNINDMAYTPQAISIGPFHHGQKEFMAMEQLKLRF 87

Query: 65  LDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIY 124
           LD+YLRR + + +EDA + A+ WE++AR+ YAE I M SD+FVKMMLVDG F+VE + ++
Sbjct: 88  LDAYLRR-VGMGIEDAFEIAQGWETRARKCYAEHIDMKSDNFVKMMLVDGAFLVEFIRMH 147

Query: 125 YGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFI 184
           Y     TQ  L+ + F+A+ +D+YRDL +LENQLPFF+LE L        +K ++   F+
Sbjct: 148 YQWATMTQPNLNYTLFQAIHVDIYRDLILLENQLPFFILECLL-------DKCSSSTPFV 207

Query: 185 QLTHKFF-----SIGLIKDNYELPHDVSTQKINHLLDFLRFYY----VPSKSVKESGEKR 244
             T  F      +  LI D       + T+K NHL+DFL FYY    V  K+ K    KR
Sbjct: 208 LFTSTFCRWYTGARELISDK------LLTKKPNHLVDFLSFYYALPTVTGKNDKLKYNKR 267

Query: 245 EFLLPPTITELCEAGVIVKKATKGKRL-MDISFQDGVLEIPHFEVHDHFETYVRNLIAFE 304
           E   PPT TEL EAGV  +KAT+ KRL MDI F+DGVL IPH E+HD FETYVRNL+A+E
Sbjct: 268 E--SPPTATELWEAGVEFQKATEDKRLIMDIRFKDGVLSIPHLEIHDAFETYVRNLLAYE 327

Query: 305 HYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTT 364
           HY +G+DER +I YV FLD LISTE+D SLLVKA IITN+IGG++E++SKLFN+LCKD  
Sbjct: 328 HYHIGDDERCLIQYVYFLDELISTERDVSLLVKAGIITNNIGGNNEDVSKLFNDLCKDIN 387

Query: 365 IPSDFYYYA-TSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTLS 421
           I  DFYYYA  S  LH +C T  HR MASLRRDYFNTPWA ISF AATFL+LLT +Q + 
Sbjct: 388 ISCDFYYYADISMDLHKYCETWWHRSMASLRRDYFNTPWAFISFLAATFLVLLTSMQAIY 438

BLAST of Lag0011429 vs. ExPASy TrEMBL
Match: A0A6J1DXD6 (UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 3.0e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 31  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 90

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 91  LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 150

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 151 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 210

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 211 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 270

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 271 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 330

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 331 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 390

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 391 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 450

BLAST of Lag0011429 vs. ExPASy TrEMBL
Match: A0A6J1E120 (UPF0481 protein At3g47200-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 3.0e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 54  VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 113

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 114 LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 173

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 174 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 233

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 234 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 293

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 294 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 353

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 354 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 413

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 414 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 473

BLAST of Lag0011429 vs. ExPASy TrEMBL
Match: A0A6J1DYL4 (UPF0481 protein At3g47200-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025435 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 3.0e-118
Identity = 248/432 (57.41%), Postives = 303/432 (70.14%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           + SSIK+MLQ+LPP+AEEC+I+RVP RL   N  AY PQ+ISIGPFHH  +DLM  E+ K
Sbjct: 9   VCSSIKKMLQELPPLAEECNIHRVPRRLLKRNLQAYMPQIISIGPFHHGRQDLMPMEQHK 68

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
           LR LD YLRR     +E  V   +SWE+ AR  YAEPI M+SD+FVKMMLVDGCFIVE+M
Sbjct: 69  LRFLDRYLRR-TNFGIEVTVGIVRSWETTARNCYAEPINMDSDEFVKMMLVDGCFIVELM 128

Query: 122 IIYYGGCFETQNGLDPSFFKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRV 181
           ++      ET+   DP  F A+  DLY DL MLENQLPFFVL+ LF+    +       +
Sbjct: 129 MMVCRIGSETETRFDPLLFCAMMTDLYCDLIMLENQLPFFVLQGLFDQFSLEA-----GL 188

Query: 182 SFIQLTHKFFSIG-LIKD-NYELPHDV--STQKINHLLDFLRFYYVP--------SKSVK 241
           SF+QLTH F++ G LIK    ELPH V  ST K+NHL+DFL FYY P        S S+ 
Sbjct: 189 SFLQLTHMFYTRGPLIKPRTLELPHGVMISTHKVNHLVDFLSFYYAPAPASVSFTSHSLA 248

Query: 242 ESGEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRN 301
            S +K  F  PPT+TEL EAG++ KKA + K +MDISF+D VL+IP  E+ D FETYVRN
Sbjct: 249 ISRKKCTF--PPTVTELWEAGIVFKKAMRAKHIMDISFKDRVLQIPPLEIGDVFETYVRN 308

Query: 302 LIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNL 361
           L+AFE Y   ND +Y I Y  FL+GLIS E+D SLLVKA IITN IGG+++E+S LFN+L
Sbjct: 309 LMAFEQY--HNDGKYAIQYFLFLNGLISREQDVSLLVKANIITNCIGGNNQEVSTLFNDL 368

Query: 362 CKDTTIPSDF-YYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTF 421
           CKD  +  D   +   ++ALH+HC  R ++ MASLRRDYFNTPWA ISF AA FLILLTF
Sbjct: 369 CKDVIVRGDCNCFNHINEALHEHCGARWNKRMASLRRDYFNTPWAFISFVAAAFLILLTF 428

BLAST of Lag0011429 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 255.4 bits (651), Expect = 8.1e-68
Identity = 158/418 (37.80%), Postives = 239/418 (57.18%), Query Frame = 0

Query: 2   LASSIKQMLQQLPPIAEECSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELK 61
           L  SIK  L  L  ++ +C IY+VP +LR +N  AYTP+++S GP H   ++L A E+ K
Sbjct: 275 LVDSIKAKLAFLSSLSTKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQK 334

Query: 62  LRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVM 121
            R L S++ R     +ED V+ A++WE  AR  YAE + ++SD+FV+M++VDG F+VE++
Sbjct: 335 YRYLLSFIPR-TNSSLEDLVRLARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVELL 394

Query: 122 IIYYGGCFETQNGLDPSFFKALRI-DLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNR 181
           +  +      +N  D  F  ++ I D+ RD+ ++ENQLPFFV++++F +L    ++    
Sbjct: 395 LRSHYPRLRGEN--DRIFGNSMMITDVCRDMILIENQLPFFVVKEIFLLLLNYYQQGT-- 454

Query: 182 VSFIQLTHKFFSIGLIKDNYELPHDVSTQKINHLLDFLRFYYVPSKSVKESGEKREFLLP 241
            S IQL  + FS  L +    +  +    +  H +D LR  Y+P   +K      +    
Sbjct: 455 PSIIQLAQRHFSYFLSR----IDDEKFITEPEHFVDLLRSCYLPQFPIKLEYTTVKVDNA 514

Query: 242 PTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFEHYPVGN 301
           P  TEL  AGV  K A     L+DISF DGVL+IP   V D  E+  +N+I FE     N
Sbjct: 515 PEATELHTAGVRFKPAETSSCLLDISFADGVLKIPTIVVDDLTESLYKNIIGFEQCRCSN 574

Query: 302 DERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTTIPSDFY 361
             +  + Y+  L   I +  DA LL+ + II N++G S  ++S LFN++ K+      FY
Sbjct: 575 --KNFLDYIMLLGCFIKSPTDADLLIHSGIIVNYLGNS-VDVSNLFNSISKEVIYDRRFY 634

Query: 362 YYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTLSSFLSL 419
           +   S+ L  +C T  +RW A LRRDYF+ PWA  S  AA  L+LLTF+Q++ S L+L
Sbjct: 635 FSMLSENLQAYCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCSILAL 680

BLAST of Lag0011429 vs. TAIR 10
Match: AT5G11290.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-52
Identity = 129/364 (35.44%), Postives = 203/364 (55.77%), Query Frame = 0

Query: 58  EELKLRCLDSYLRRDIKLKVEDAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFI 117
           E+ KLR L S++ R   L +ED V+ A++WE +AR  Y E + ++SD++VKM++VD  F+
Sbjct: 2   EDHKLRYLQSFIPR-TALSLEDLVRVARTWEERARFCYTEDVRLSSDEYVKMLIVDASFL 61

Query: 118 VEVMIIYYGGCFETQNGLDPSFF--KALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGE 177
           VE+++      F+   G+    +  + + +D+  D+ +LENQLP+FV+E +F +L  D  
Sbjct: 62  VELLL---RSQFDVYRGMLDRIYGKQKMIVDVNHDVMLLENQLPYFVVEGMFGLLHVDYH 121

Query: 178 KNNNRVSFIQLTH-KFFSIGLIKDNYELPHDVSTQKINHLLDFLRFYYVPSKSVKESGEK 237
           +    ++ I   H K F + +          +S  KI H +D LR  ++P       G  
Sbjct: 122 RELPPLTRIIHNHFKKFWMSI----PSFSRSISDSKICHFVDLLRSIHLPLVLSFPGGSM 181

Query: 238 REFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFE 297
           R      +  E+  AGV ++ A      +DISF +GVL IP  +++D  E+  RN+I FE
Sbjct: 182 RMMDSVLSAKEIQNAGVKLQPADNNTCALDISFANGVLTIPKIKINDITESLYRNIILFE 241

Query: 298 HYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTT 357
                  + Y IHY+ FL   I +  DA L +   II N  G + E++S+LFN++ K+T+
Sbjct: 242 Q--CHRLDAYFIHYMRFLSCFIRSPMDAELFIDHGIIVNRFGNA-EDVSRLFNSILKETS 301

Query: 358 IPSDFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTLSS 417
             S FYY      L  HC    ++W A+LRRDYF+ PW++ S  AA  L+LLTF+Q + S
Sbjct: 302 Y-SGFYYKTVYGNLQAHCNAPWNKWKATLRRDYFHNPWSAASVVAACVLLLLTFVQAICS 353

Query: 418 FLSL 419
            L+L
Sbjct: 362 ILAL 353

BLAST of Lag0011429 vs. TAIR 10
Match: AT2G36430.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 193.4 bits (490), Expect = 3.8e-49
Identity = 130/407 (31.94%), Postives = 206/407 (50.61%), Query Frame = 0

Query: 20  CSIYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRRDIKLKVED 79
           CSI+RVP  + + N   Y P+V+SIGP+H     L   EE K R L+  L R   L +ED
Sbjct: 45  CSIFRVPQSMIDCNGRCYEPRVVSIGPYHRGQTQLKMIEEHKWRYLNVLLTRTQNLTLED 104

Query: 80  AVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFETQNGL---- 139
            +K+ K+ E  AR  Y+E I M+S++F +MM++DGCF++E+        F   N L    
Sbjct: 105 YMKSVKNVEEVARECYSETIHMDSEEFNEMMVLDGCFLLEL--------FRKVNNLVPFE 164

Query: 140 --DPSFFKALRID-LYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFIQLTHKFFS 199
             DP    A  +   YRD   LENQ+PFFVLE LFN+   D E N    S   L   FF+
Sbjct: 165 PNDPLVAMAWVLPFFYRDFLCLENQIPFFVLETLFNLTRGDNE-NETNASLQSLAFAFFN 224

Query: 200 IGLIKDNYELPHDVSTQKINHLLDFLRFYYVPSK------SVKESGEKREFLLPPTITEL 259
             + +   +L       +  HLLD LR  ++P        +     EK    +  +I++L
Sbjct: 225 NMMHRTEEDLAR-FKELRAKHLLDLLRSSFIPESELHTPPATNPGKEKMPSHIIHSISKL 284

Query: 260 CEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYVRNLIAFEHYPVGNDERYVI 319
             AG+ +++    +  + + F+ G +E+P   V D   +++ N +A+E   V     +  
Sbjct: 285 RRAGIKLRELKDAESFLVVRFRHGTIEMPAITVDDFMSSFLENCVAYEQCHVACSMHFTT 344

Query: 320 HYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFNNLCKDTTIP-SDFYYYATS 379
            Y   LD L +T KD   L    II N+  G+D E++K  N+L +D     +  Y     
Sbjct: 345 -YATLLDCLTNTYKDVEYLCDQNIIENYF-GTDTELAKFVNSLGRDVAFDITQCYLKDLF 404

Query: 380 KALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILLTFLQTL 413
           + ++++  +  H   A+ +  YFN+PW+ +S  AA  L++L+ +QT+
Sbjct: 405 EEVNEYYKSSWHVEWATFKFTYFNSPWSFVSALAALVLLVLSVIQTI 439

BLAST of Lag0011429 vs. TAIR 10
Match: AT3G50120.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 189.9 bits (481), Expect = 4.2e-48
Identity = 146/431 (33.87%), Postives = 219/431 (50.81%), Query Frame = 0

Query: 22  IYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRR---DIKLKVE 81
           IYRVP  L+  ++ +Y PQ +S+GP+HH  K L + +  K R ++  L+R    IK+ + 
Sbjct: 105 IYRVPYYLQENDNKSYFPQTVSLGPYHHGKKRLRSMDRHKWRAVNRVLKRTNQGIKMYI- 164

Query: 82  DAVKNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFE--TQNGL- 141
           DA++     E KAR  Y  P++++S++F++M+++DGCF++E+    + G  E  T+ G  
Sbjct: 165 DAMREL---EEKARACYEGPLSLSSNEFIEMLVLDGCFVLEL----FRGAVEGFTELGYA 224

Query: 142 --DPSF-FKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFIQLTHKFF- 201
             DP F  +     + RD+ MLENQLP FVL +L  +  Q G +N   +   QL  +FF 
Sbjct: 225 RNDPVFAMRGSMHSIQRDMVMLENQLPLFVLNRLLEL--QLGTRNQTGL-VAQLAIRFFD 284

Query: 202 ----------SIGLIKDNYELPHDVSTQKIN-----HLLDFLRFYYVPSKSVKES----- 261
                       G  K    L  D S          H LD  R   + S    E      
Sbjct: 285 PLMPTDEPLTKSGQSKLENSLARDKSFDPFADMGELHCLDVFRRSLLRSSPKPEPRLTRK 344

Query: 262 --------GEKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHF 321
                    +KR   L   +TEL EAG+  ++  K  R  D+ F++G LEIP   +HD  
Sbjct: 345 RWSRNTRVADKRRQQLIHCVTELKEAGIKFRR-RKTDRFWDMQFKNGYLEIPRLLIHDGT 404

Query: 322 ETYVRNLIAFE--HYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEE 381
           ++   NLIAFE  H    ND   +  Y+ F+D LI + +D S L    II  H  GSD E
Sbjct: 405 KSLFLNLIAFEQCHIDSSND---ITSYIIFMDNLIDSHEDVSYLHYCGII-EHWLGSDSE 464

Query: 382 ISKLFNNLCKDTTIPS-DFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAA 412
           ++ LFN LC++    + D Y    S  ++ +   + + W A+L+  YFN PWA +SF AA
Sbjct: 465 VADLFNRLCQEVVFDTEDSYLSRLSIEVNRYYDHKWNAWRATLKHKYFNNPWAIVSFCAA 519

BLAST of Lag0011429 vs. TAIR 10
Match: AT3G50170.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 189.5 bits (480), Expect = 5.5e-48
Identity = 142/425 (33.41%), Postives = 214/425 (50.35%), Query Frame = 0

Query: 22  IYRVPTRLRNINHIAYTPQVISIGPFHHWPKDLMATEELKLRCLDSYLRRDIKLKVEDAV 81
           IYRVP  L+  +  +Y PQ +S+GP+HH  K L   E  K R L+  L+R +K ++E   
Sbjct: 115 IYRVPHYLQENDKKSYFPQTVSLGPYHHGKKRLRPMERHKWRALNKVLKR-LKQRIEMYT 174

Query: 82  KNAKSWESKARRFYAEPITMNSDDFVKMMLVDGCFIVEVMIIYYGGCFE--TQNGL---D 141
              +  E KAR  Y  PI+++ ++F +M+++DGCF++E+    + G  E  T+ G    D
Sbjct: 175 NAMRELEEKARACYEGPISLSRNEFTEMLVLDGCFVLEL----FRGTVEGFTEIGYARND 234

Query: 142 PSF-FKALRIDLYRDLTMLENQLPFFVLEQLFNMLPQDGEKNNNRVSFIQLTHKFFSIGL 201
           P F  + L   + RD+ MLENQLP FVL++L  +  Q G +N   +    +  KFF   L
Sbjct: 235 PVFAMRGLMHSIQRDMIMLENQLPLFVLDRLLEL--QLGTQNQTGI-VAHVAVKFFD-PL 294

Query: 202 IKDNYELPHDVSTQKIN---------------HLLDFLRFYYVPSKSVKESG-------- 261
           +     L     ++ +N               H LD  R   + S     +         
Sbjct: 295 MPTGEALTKPDQSKLMNWLEKSLDTLGDKGELHCLDVFRRSLLQSSPTPNTRSLLKRLTR 354

Query: 262 -----EKREFLLPPTITELCEAGVIVKKATKGKRLMDISFQDGVLEIPHFEVHDHFETYV 321
                +KR+  L   +TEL EAGV  +K  K  R  DI F++G LEIP   +HD  ++  
Sbjct: 355 NTRVVDKRQQQLVHCVTELREAGVKFRK-RKTDRFWDIEFKNGYLEIPKLLIHDGTKSLF 414

Query: 322 RNLIAFEHYPVGNDERYVIHYVEFLDGLISTEKDASLLVKAKIITNHIGGSDEEISKLFN 381
            NLIAFE   +     ++  Y+ F+D LI++ +D S L    II  H  GSD E++ LFN
Sbjct: 415 SNLIAFEQCHI-ESSNHITSYIIFMDNLINSSEDVSYLHYCGII-EHWLGSDSEVADLFN 474

Query: 382 NLCKDTTI-PSDFYYYATSKALHDHCLTRRHRWMASLRRDYFNTPWASISFTAATFLILL 412
            LC++    P D +    S  ++ +   + +   A+L   YFN PWA  SF+AA  L+LL
Sbjct: 475 RLCQEVVFDPKDSHLSRLSGDVNRYYNRKWNVLKATLTHKYFNNPWAYFSFSAAVILLLL 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132118.12.5e-12760.00UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022132066.19.5e-11957.14UPF0481 protein At3g47200-like [Momordica charantia][more]
XP_022158990.16.1e-11857.41UPF0481 protein At3g47200-like isoform X2 [Momordica charantia][more]
XP_022158992.16.1e-11857.41UPF0481 protein At3g47200-like isoform X3 [Momordica charantia][more]
XP_022158989.16.1e-11857.41UPF0481 protein At3g47200-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SD536.1e-3629.81UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8972.3e-1429.03Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1BVD41.2e-12760.00UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005065 PE... [more]
A0A6J1BR714.6e-11957.14UPF0481 protein At3g47200-like OS=Momordica charantia OX=3673 GN=LOC111005028 PE... [more]
A0A6J1DXD63.0e-11857.41UPF0481 protein At3g47200-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1E1203.0e-11857.41UPF0481 protein At3g47200-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DYL43.0e-11857.41UPF0481 protein At3g47200-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G31980.18.1e-6837.80unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT5G11290.12.8e-5235.44Plant protein of unknown function (DUF247) [more]
AT2G36430.13.8e-4931.94Plant protein of unknown function (DUF247) [more]
AT3G50120.14.2e-4833.87Plant protein of unknown function (DUF247) [more]
AT3G50170.15.5e-4833.41Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 22..406
e-value: 1.8E-109
score: 366.7
NoneNo IPR availablePANTHERPTHR31170:SF13BNAC04G53230D PROTEINcoord: 2..417
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 2..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011429.1Lag0011429.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane