Lag0011377 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011377
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ATP-dependent DNA helicase At3g02060, chloroplastic
Location	chr1: 23196798 .. 23197507 (-)
RNA-Seq Expression	Lag0011377
Synteny	Lag0011377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGTCTCTCAGATCTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAAATATGGCAGAGTATTGCCCTCTCTCTATCTCTCTTTCTTATTCCCAATCTCTTTCTCCTATCTCTCTTTTTCCCCTTTTATGATTTTGGGTTTTGTTCTTTGTTGCAGTACAATGAAATTTTGGGAAAAGGTGCTTCAAAAACAGTGTAGGGATTTGATTTTCTTGCATCTTATGGGCTTCCCATTTTGCCATTTGCTAGAAATTTGCATATTCTTCTGAAGCATTTTAATGGGTTTTTGTTTGTTAATTTATTACAATTTATGCGCATTTTGTTACTTGCGTTGCTTTCAATTGAGGTAATTGGATTTGTTTGTCTCTTAGGTTAGTGATTTTCGAGGTTCCCAAAATTTTCCTGGAGTCTTTTTTATTTTCTTGGAAGAGTCTTTTTGAGATCTGAAATGGAATTTTGAAAAATGTTATTTCGATGCTTCTATATTATTTTTTCTGGACTTTTGTGGACTAATTGTTTTAGAGCAATATGGCATTAACCAAGCAATGGTTTTAATTATAAGGTTGATCCTTATACGCTTCGTTCTGGGGATTATGTTGTGCATAAGAAGGTCGGTATCGGTCGATTCGTTGGAATTAAATTTGATGTTCAGAAGGGTTTTACGGAGCCTATTGAGTATGTGTTCATTGAATAG

mRNA sequence

ATGGATGGTCTCTCAGATCTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAAATATGGCAGAGTTGATCCTTATACGCTTCGTTCTGGGGATTATGTTGTGCATAAGAAGGTCGGTATCGGTCGATTCGTTGGAATTAAATTTGATGTTCAGAAGGGTTTTACGGAGCCTATTGAGTATGTGTTCATTGAATAG

Coding sequence (CDS)

ATGGATGGTCTCTCAGATCTTGAGCCTGATTGCTCTGAATTTGTTGAAGTTGATCCCACTGGCAAATATGGCAGAGTTGATCCTTATACGCTTCGTTCTGGGGATTATGTTGTGCATAAGAAGGTCGGTATCGGTCGATTCGTTGGAATTAAATTTGATGTTCAGAAGGGTTTTACGGAGCCTATTGAGTATGTGTTCATTGAATAG

Protein sequence

MDGLSDLEPDCSEFVEVDPTGKYGRVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE

Homology

[Close]

BLAST of Lag0011377 vs. NCBI nr
Match: XP_038907245.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 91.7 bits (226), Expect = 2.7e-15
Identity = 44/51 (86.27%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 18  DPTGKYGRVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           D  G   +VDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKG TEPIEYVFIE
Sbjct: 131 DSDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181

[Close]

BLAST of Lag0011377 vs. NCBI nr
Match: XP_038907244.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 91.7 bits (226), Expect = 2.7e-15
Identity = 44/51 (86.27%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 18  DPTGKYGRVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           D  G   +VDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKG TEPIEYVFIE
Sbjct: 131 DSDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 181

[Close]

BLAST of Lag0011377 vs. NCBI nr
Match: XP_022141425.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 90.9 bits (224), Expect = 4.6e-15
Identity = 42/44 (95.45%), Postives = 43/44 (97.73%), Query Frame = 0


Query: 25  RVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           +VDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKG TEPIEYVFIE
Sbjct: 137 KVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 180

[Close]

BLAST of Lag0011377 vs. NCBI nr
Match: XP_022141422.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 90.9 bits (224), Expect = 4.6e-15
Identity = 42/44 (95.45%), Postives = 43/44 (97.73%), Query Frame = 0


Query: 25  RVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           +VDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKG TEPIEYVFIE
Sbjct: 137 KVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 180

[Close]

BLAST of Lag0011377 vs. NCBI nr
Match: XP_022141424.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 90.9 bits (224), Expect = 4.6e-15
Identity = 42/44 (95.45%), Postives = 43/44 (97.73%), Query Frame = 0


Query: 25  RVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           +VDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKG TEPIEYVFIE
Sbjct: 137 KVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIE 180

[Close]

BLAST of Lag0011377 vs. ExPASy Swiss-Prot
Match: F4JFJ3 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g02060 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.8e-14
Identity = 36/44 (81.82%), Postives = 40/44 (90.91%), Query Frame = 0


Query: 25  RVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGFTEPIEYVFIE 69
           +VDPY+L SGDYVVHKKVGIGRFVGIKFDV K  +EP+EYVFIE
Sbjct: 136 KVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEYVFIE 179

[Close]

BLAST of Lag0011377 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 0


Query: 1  MDGLSDLEPDCSEFVEVDPTGKYGRVDPYTLRSGDYVVHK 41
          M+ LS LEPD SEFVEVDPTG+YGR +    +     V++
Sbjct: 1  MNNLSYLEPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYR 40

[Close]

BLAST of Lag0011377 vs. ExPASy Swiss-Prot
Match: Q2V338 (Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN=WNK9 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.0e-04
Identity = 22/51 (43.14%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 1  MDGLSDLEPDCSEFVEVDPTGKYGRVDPYTLRSGDYVVHKKVGIGRFVGIK 52
          M+ LS LE D SE+VEVDPTG+YGR +    +     V++  G   + GI+
Sbjct: 2  MNNLSHLESDYSEYVEVDPTGRYGRYNEVLGKGSSKTVYR--GFDEYQGIE 50

The following BLAST results are available for this feature:

BLAST of Lag0011377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907245.1	2.7e-15	86.27	ATP-dependent DNA helicase At3g02060, chloroplastic isoform X2 [Benincasa hispid...	[more]
XP_038907244.1	2.7e-15	86.27	ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Benincasa hispid...	[more]
XP_022141425.1	4.6e-15	95.45	ATP-dependent DNA helicase At3g02060, chloroplastic isoform X3 [Momordica charan...	[more]
XP_022141422.1	4.6e-15	95.45	ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Momordica charan...	[more]
XP_022141424.1	4.6e-15	95.45	ATP-dependent DNA helicase At3g02060, chloroplastic isoform X2 [Momordica charan...	[more]

BLAST of Lag0011377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFJ3	1.8e-14	81.82	ATP-dependent DNA helicase At3g02060, chloroplastic OS=Arabidopsis thaliana OX=3...	[more]
Q9CAV6	1.3e-04	52.50	Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=...	[more]
Q2V338	5.0e-04	43.14	Probable serine/threonine-protein kinase WNK9 OS=Arabidopsis thaliana OX=3702 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003711	CarD-like/TRCF domain	PFAM	PF02559	CarD_CdnL_TRCF	coord: 31..67 e-value: 6.1E-6 score: 26.8
None	No IPR available	GENE3D	2.40.10.170		coord: 30..68 e-value: 4.4E-7 score: 31.6
IPR036101	CarD-like/TRCF domain superfamily	SUPERFAMILY	141259	CarD-like	coord: 25..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0011377.1	Lag0011377.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0016787	hydrolase activity