Lag0011375 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011375
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCCHC-type domain-containing protein
Locationchr1: 23126981 .. 23127827 (+)
RNA-Seq ExpressionLag0011375
SyntenyLag0011375
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAAAAGTCAGAGGAAGATGCAATTTTACATCAGTTTGCGGAGATGAAGGTTACGGCTGAGGAAAAGGCTAGTGTCTTTCACCAGCAAGAAGAAGCAATTGATGTCACGGAAAAGAAGTTGGTGAATGTCGTCTTATGCAAGATTTTTACAAAGAAAAAAATTACTCCCGAGGTATTCAAATCAATGATGCCAAAAATTTGAAATCAGGAGCATACGATTATTGACTACAGAGGATTCAATTTATTCCTTTGTAAGTTTAAAAACGCTCGAATCAAAAGTCACATTGTGGAGTCAGGGCCTTGGTTTTATGATAGAACAATGTTGTTGCTTGCTGATCCTAAAGGCGACTGCAGTGGGGACGAAATGGAGTTCAGGTATGTTTCTTTCTGGGTTCATTTTCATAAACTAACTGCCTTTAGCTTGCTTTTCCAGGTCTGCAGCAATGGAGATCAGAAGTTTAGTGGGTAAGGTGGAACAAGTGGACCTTGAAGATGTGTCTGATGAGAACTGGGGAAGCTCATTACGGATTAAGATCCAAGTAGACATAACAAGTCCTTTGAAGAGGGGAGTGTTCTTGAAGTCAGTACGAGCTGGTAATGAGAAATGGATAACAATGACTTATGAAAAACTCCCTGATTTTTGCTATGGCTGTGGTGGGTTGGGTCACATAATTAAAGAATGTGAGGAAGACACAGGTTCAGATACTGAGGATATCCCGTATGGACGGTGGATGAGGGAACCAACTAAGCTTGTTAGCAAGGGACCATTCGGTGCTCAGGGGGAGGAGAACAAGTTTGGGGGAGGGGGAAGAGGTCGAGGGAGGGAGAGGTTGGAGGTTTGA

mRNA sequence

ATGACGAAAAAGTCAGAGGAAGATGCAATTTTACATCAGTTTGCGGAGATGAAGGTTACGGCTGAGGAAAAGGCTAGTGTCTTTCACCAGCAAGAAGAAGCAATTGATGTCACGGAAAAGAAGTTGGAGCATACGATTATTGACTACAGAGGATTCAATTTATTCCTTTGTAAGTTTAAAAACGCTCGAATCAAAAGTCACATTGTGGAGTCAGGGCCTTGGTTTTATGATAGAACAATGTTGTTGCTTGCTGATCCTAAAGGCGACTGCAGTGGGGACGAAATGGAGTTCAGGTCTGCAGCAATGGAGATCAGAAGTTTAGTGGGTAAGGTGGAACAAGTGGACCTTGAAGATGTGTCTGATGAGAACTGGGGAAGCTCATTACGGATTAAGATCCAAGTAGACATAACAAGTCCTTTGAAGAGGGGAGTGTTCTTGAAGTCAGTACGAGCTGGTAATGAGAAATGGATAACAATGACTTATGAAAAACTCCCTGATTTTTGCTATGGCTGTGGTGGGTTGGGTCACATAATTAAAGAATGTGAGGAAGACACAGGTTCAGATACTGAGGATATCCCGTATGGACGGTGGATGAGGGAACCAACTAAGCTTGTTAGCAAGGGACCATTCGGTGCTCAGGGGGAGGAGAACAAGTTTGGGGGAGGGGGAAGAGGTCGAGGGAGGGAGAGGTTGGAGGTTTGA

Coding sequence (CDS)

ATGACGAAAAAGTCAGAGGAAGATGCAATTTTACATCAGTTTGCGGAGATGAAGGTTACGGCTGAGGAAAAGGCTAGTGTCTTTCACCAGCAAGAAGAAGCAATTGATGTCACGGAAAAGAAGTTGGAGCATACGATTATTGACTACAGAGGATTCAATTTATTCCTTTGTAAGTTTAAAAACGCTCGAATCAAAAGTCACATTGTGGAGTCAGGGCCTTGGTTTTATGATAGAACAATGTTGTTGCTTGCTGATCCTAAAGGCGACTGCAGTGGGGACGAAATGGAGTTCAGGTCTGCAGCAATGGAGATCAGAAGTTTAGTGGGTAAGGTGGAACAAGTGGACCTTGAAGATGTGTCTGATGAGAACTGGGGAAGCTCATTACGGATTAAGATCCAAGTAGACATAACAAGTCCTTTGAAGAGGGGAGTGTTCTTGAAGTCAGTACGAGCTGGTAATGAGAAATGGATAACAATGACTTATGAAAAACTCCCTGATTTTTGCTATGGCTGTGGTGGGTTGGGTCACATAATTAAAGAATGTGAGGAAGACACAGGTTCAGATACTGAGGATATCCCGTATGGACGGTGGATGAGGGAACCAACTAAGCTTGTTAGCAAGGGACCATTCGGTGCTCAGGGGGAGGAGAACAAGTTTGGGGGAGGGGGAAGAGGTCGAGGGAGGGAGAGGTTGGAGGTTTGA

Protein sequence

MTKKSEEDAILHQFAEMKVTAEEKASVFHQQEEAIDVTEKKLEHTIIDYRGFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSAAMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTEDIPYGRWMREPTKLVSKGPFGAQGEENKFGGGGRGRGRERLEV
Homology
BLAST of Lag0011375 vs. NCBI nr
Match: XP_024642377.1 (uncharacterized protein LOC112422874 [Medicago truncatula])

HSP 1 Score: 123.2 bits (308), Expect = 2.9e-24
Identity = 66/173 (38.15%), Postives = 92/173 (53.18%), Query Frame = 0

Query: 53  NLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSA------------ 112
           NLFL KF + R   +I+ SGPW +DR +L+L     +    E++  +             
Sbjct: 62  NLFLFKFVSRRDAENILHSGPWSFDRNLLILDTISSEEQPSELDLHTVQFWVRIYDLPLK 121

Query: 113 ------AMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNE 172
                 A ++  LVGK  +VD  D +    G S+RIK+ +D+ +PLKRG+ LK  +    
Sbjct: 122 LRSDLMARKLGDLVGKFLEVDQRDTN--IMGKSIRIKVDIDLRNPLKRGIVLKYQK--RS 181

Query: 173 KWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTE--------DIPYGRWMR 200
            WI   YE+LP+FCY CG +GH +KECEE+TG D E        D  YG WMR
Sbjct: 182 LWIFFKYERLPNFCYKCGRIGHQMKECEEETGQDGEGYTDVEEQDQAYGSWMR 230

BLAST of Lag0011375 vs. NCBI nr
Match: XP_035547259.1 (uncharacterized protein LOC118348856 [Juglans regia])

HSP 1 Score: 118.2 bits (295), Expect = 9.2e-23
Identity = 61/157 (38.85%), Postives = 92/157 (58.60%), Query Frame = 0

Query: 51  GFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSAAMEIRSLVGK 110
           G NLFL +F++ R K  I+  GPW +D  ++LL   +G+  G  +        I + +G 
Sbjct: 75  GSNLFLVEFEDDRDKQRILWEGPWSFDCYLVLLKQLEGNYKGGRL--------IGNKIGI 134

Query: 111 VEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNEKWITMTYEKLPDFCYG 170
           VE+VD E  S+  WG  LRI+++V+I+ PL RG+ +K  + G+  WI   YE+LP+FCY 
Sbjct: 135 VEEVDGEQ-SEVAWGEYLRIRVRVNISQPLMRGITVKLGQFGS-VWIRFAYERLPNFCYM 194

Query: 171 CGGLGHIIKECE---EDTGSDTEDIPYGRWMREPTKL 205
           CG LGH  K+CE        DT+ +P+G W+   T +
Sbjct: 195 CGKLGHQQKDCEINGTQGARDTDQLPFGPWLHVGTTM 221

BLAST of Lag0011375 vs. NCBI nr
Match: XP_024036844.1 (uncharacterized protein LOC112096879 [Citrus clementina])

HSP 1 Score: 116.7 bits (291), Expect = 2.7e-22
Identity = 57/170 (33.53%), Postives = 90/170 (52.94%), Query Frame = 0

Query: 47  IDYRGFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEF--------- 106
           ID  G N+F+ KF     K  ++  GPW +DR +++L +PKG     +  F         
Sbjct: 72  IDSLGNNIFMFKFAKEADKKRVLSGGPWHFDRALIVLTEPKGIGEVSKQAFTDVPFWVQI 131

Query: 107 ---------RSAAMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKS 166
                    R    E+  ++GKVE+++ ++  D  +G   RI+I ++IT PLK+ +FLK 
Sbjct: 132 RNIPMACMERDFLQELEGMIGKVEEIETDENGD-CFGEFARIRIFINITQPLKKILFLKQ 191

Query: 167 VRAGNEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTEDIPYGRWM 199
                +  + + YE+LPDFC+ CG +GH  KEC +  G   ED+ +G WM
Sbjct: 192 -EGETDIPMPLVYERLPDFCFCCGIIGHQFKECAQYQGQPKEDLSFGMWM 239

BLAST of Lag0011375 vs. NCBI nr
Match: XP_024033132.1 (uncharacterized protein LOC112095437 [Citrus clementina])

HSP 1 Score: 114.8 bits (286), Expect = 1.0e-21
Identity = 59/184 (32.07%), Postives = 96/184 (52.17%), Query Frame = 0

Query: 34  AIDVTEKKLEHTIIDYRGFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGD 93
           A+D   + +    ++  G N+F+ KF +   K  +   GPW +DR +++L +PKG  S  
Sbjct: 59  ALDQAWRTVHDFKVESLGSNIFVFKFLSEADKKRVFNGGPWHFDRALMVLQEPKGIGSIK 118

Query: 94  EMEF------------------RSAAMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVD 153
           +  F                   S   E+ S VGKVE + + D   E +G  +RI++ V+
Sbjct: 119 KQSFSHVSFWIRIHNVPLMCMDTSIIRELGSRVGKVEDIGM-DAQGECFGEFVRIQVSVN 178

Query: 154 ITSPLKRGVFLKSVRAGNEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTEDIPYG 200
           IT PLK+ + LK      +  + + YE+LPDFC+ CG +GH  +EC E  G   E++P+G
Sbjct: 179 ITKPLKKLIVLKQ-EDDEDIPLPVVYERLPDFCFCCGCIGHQFRECMEYKGQQKENLPFG 238

BLAST of Lag0011375 vs. NCBI nr
Match: XP_006485824.1 (uncharacterized protein LOC102613298 [Citrus sinensis])

HSP 1 Score: 114.8 bits (286), Expect = 1.0e-21
Identity = 71/234 (30.34%), Postives = 118/234 (50.43%), Query Frame = 0

Query: 1   MTKKSEEDAILHQFAEMKVTAE------------EKASVFHQ-QEEAIDVTEKKLEHTII 60
           +T +SEED ++     MKV  E            +  SV  +    A+    +  + T +
Sbjct: 13  ITLQSEEDNMISFGRNMKVRGERLVAHCLVGKILQTRSVPREGLRAAMQQAWRTTKETKV 72

Query: 61  DYRGFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSA------- 120
           +  G N+FL KF +   K  I+  GPW +DR+++++ +P G     + +F  A       
Sbjct: 73  ESLGDNIFLFKFASKSEKKRILFGGPWHFDRSLMVVIEPMGIGDVKKQDFTHALFWVQIH 132

Query: 121 -----------AMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSV 180
                        EI   +GKV++V+ +D + E  GS +R++I V++T PL + + LK +
Sbjct: 133 NIPIMCMDKEIIQEIGGRIGKVQEVETDD-AGECMGSFVRVRIMVNVTRPLPKRLLLK-L 192

Query: 181 RAGNEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTEDIPYGRWMREPTK 204
             G +  + + YEKLPDFC+ CG +GH  +EC +  G   +D+ YG WMR  T+
Sbjct: 193 EDGGQISLRIAYEKLPDFCFCCGLIGHQFRECLDYKGQSKDDLTYGAWMRAQTR 244

BLAST of Lag0011375 vs. ExPASy TrEMBL
Match: A0A6P9EID8 (uncharacterized protein LOC118348856 OS=Juglans regia OX=51240 GN=LOC118348856 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 4.5e-23
Identity = 61/157 (38.85%), Postives = 92/157 (58.60%), Query Frame = 0

Query: 51  GFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSAAMEIRSLVGK 110
           G NLFL +F++ R K  I+  GPW +D  ++LL   +G+  G  +        I + +G 
Sbjct: 75  GSNLFLVEFEDDRDKQRILWEGPWSFDCYLVLLKQLEGNYKGGRL--------IGNKIGI 134

Query: 111 VEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNEKWITMTYEKLPDFCYG 170
           VE+VD E  S+  WG  LRI+++V+I+ PL RG+ +K  + G+  WI   YE+LP+FCY 
Sbjct: 135 VEEVDGEQ-SEVAWGEYLRIRVRVNISQPLMRGITVKLGQFGS-VWIRFAYERLPNFCYM 194

Query: 171 CGGLGHIIKECE---EDTGSDTEDIPYGRWMREPTKL 205
           CG LGH  K+CE        DT+ +P+G W+   T +
Sbjct: 195 CGKLGHQQKDCEINGTQGARDTDQLPFGPWLHVGTTM 221

BLAST of Lag0011375 vs. ExPASy TrEMBL
Match: A0A7N2MS75 (CCHC-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 6.4e-22
Identity = 62/193 (32.12%), Postives = 101/193 (52.33%), Query Frame = 0

Query: 54  LFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEF---------------- 113
           L+L +F + R K  I+E  PW Y+++++LL + +G+    ++                  
Sbjct: 78  LYLVEFGDGRDKKRILEMCPWTYEKSLILLKEFEGEQVPKDISLWQSPFWVQIHNLPLKS 137

Query: 114 --RSAAMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNEK 173
             R     I + +G+V  VD+ + S  NWG  LR+++ +D+T  L RG  +  +  G ++
Sbjct: 138 RTRETGRVIGTSLGEVMDVDVSE-SGVNWGKYLRVRVMMDVTKKLVRGKRI-VIEGGEQR 197

Query: 174 WITMTYEKLPDFCYGCGGLGHIIKECEEDTGS----DTEDIPYGRWMREPTKLVSKGPFG 224
           WIT  YE+LP+FCY CG L H +K+C+E+ GS    +  ++ YG WMR        G F 
Sbjct: 198 WITFKYERLPNFCYKCGLLNHGVKDCKEENGSGANMEHNNLQYGAWMRGEVPRKGGGDFT 257

BLAST of Lag0011375 vs. ExPASy TrEMBL
Match: A0A7N2QWW0 (CCHC-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 8.4e-22
Identity = 65/193 (33.68%), Postives = 102/193 (52.85%), Query Frame = 0

Query: 54  LFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKG-----DCSGDEMEF----------- 113
           L+L +F + R K  I+E  PW Y+++++LL + +G     D S  +  F           
Sbjct: 90  LYLVEFGDGRDKKRILEMRPWTYEKSLILLKEFEGEQVPKDISLWQSSFWVQIHNLPLKS 149

Query: 114 --RSAAMEIRSLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAGNEK 173
             R     I + +G+V  VD+ + S  NWG  LR+++ +D+T  L RG  +  +  G ++
Sbjct: 150 RTRETGRVIGTSLGEVMDVDVSE-SGVNWGKYLRVRVLMDVTKKLVRGKRI-VIEGGEQR 209

Query: 174 WITMTYEKLPDFCYGCGGLGHIIKECEEDTGS----DTEDIPYGRWMREPTKLVSKGPFG 224
           WIT  YE+LP+FCY CG L H +K+C+E+ GS    +  ++ YG WMR        G F 
Sbjct: 210 WITFKYERLPNFCYKCGLLNHGVKDCKEENGSGANLEHNNLQYGAWMRGEVPRKGGGDFT 269

BLAST of Lag0011375 vs. ExPASy TrEMBL
Match: A0A5C7HLK8 (CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_018469 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 4.2e-21
Identity = 52/175 (29.71%), Postives = 95/175 (54.29%), Query Frame = 0

Query: 47  IDYRGFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSAAMEIRS 106
           I+  G N+F+ KF N   ++ I + GPW++D+++++L   +G  +  ++ F S  M I++
Sbjct: 72  IESVGVNVFMFKFNNKEERNRIWQRGPWYFDKSLMVLVRLEGMGNISQLRFNSVEMWIQN 131

Query: 107 L------------------VGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKS 166
                              +G+V  +D+   S + WG  L++K+Q+DIT PLK+ ++L+ 
Sbjct: 132 YDVPIICMNRRLAKWMAEQIGRV--IDIPTESKDCWGKFLKVKVQIDITKPLKQWLWLRM 191

Query: 167 VRAGNEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTE----DIPYGRWMR 200
            ++     +++ YE+LP+FCY CG +GH  KECE+              +G W+R
Sbjct: 192 EQSSEIVMVSLKYERLPEFCYVCGRIGHASKECEDKEAKQEALQGVSTKFGSWIR 244

BLAST of Lag0011375 vs. ExPASy TrEMBL
Match: A0A6J1D765 (uncharacterized protein LOC111017902 OS=Momordica charantia OX=3673 GN=LOC111017902 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 4.2e-21
Identity = 70/213 (32.86%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 51  GFNLFLCKFKNARIKSHIVESGPWFYDRTMLLLADPKGDCSGDEMEFRSAAMEIR----- 110
           G N+++  FK+   KS ++ SGPW +++++L+L  P       +M F   A  I+     
Sbjct: 74  GMNIYVILFKSLSEKSRVLSSGPWTFNKSLLVLTSPTATNQPLDMNFNFCAFWIQIHNIP 133

Query: 111 -------------SLVGKVEQVDLEDVSDENWGSSLRIKIQVDITSPLKRGVFLKSVRAG 170
                        + +G VE+++  D +D   G  +R+++++D++ PL+RG+ LK+   G
Sbjct: 134 FECISTEMANILGAKLGDVEEIE-GDGADGWAGPFIRVRVKIDVSKPLRRGIKLKN-SDG 193

Query: 171 NEKWITMTYEKLPDFCYGCGGLGHIIKECEEDTGSDTEDIP--YGRWMREP--TKLVSK- 230
            + W  + YEKLPDFCY CG +GH  +ECE+ +   T + P  YG W+R     K VS  
Sbjct: 194 KDIWCPLRYEKLPDFCYECGKIGHSGRECEQRSKVVTTNSPEQYGDWLRATLLKKSVSHP 253

Query: 231 --------GPFGAQGEENKFGGGGRGRGRERLE 233
                   G FG +G +   G GGRG  R R E
Sbjct: 254 EEEVFWRGGRFG-RGHQVNGGRGGRGDWRRRDE 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_024642377.12.9e-2438.15uncharacterized protein LOC112422874 [Medicago truncatula][more]
XP_035547259.19.2e-2338.85uncharacterized protein LOC118348856 [Juglans regia][more]
XP_024036844.12.7e-2233.53uncharacterized protein LOC112096879 [Citrus clementina][more]
XP_024033132.11.0e-2132.07uncharacterized protein LOC112095437 [Citrus clementina][more]
XP_006485824.11.0e-2130.34uncharacterized protein LOC102613298 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6P9EID84.5e-2338.85uncharacterized protein LOC118348856 OS=Juglans regia OX=51240 GN=LOC118348856 P... [more]
A0A7N2MS756.4e-2232.12CCHC-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2QWW08.4e-2233.68CCHC-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A5C7HLK84.2e-2129.71CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_01846... [more]
A0A6J1D7654.2e-2132.86uncharacterized protein LOC111017902 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 134..182
e-value: 3.4E-11
score: 42.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..233
NoneNo IPR availablePANTHERPTHR31286:SF62SUBFAMILY NOT NAMEDcoord: 48..214
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 48..214
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 168..183
score: 9.438442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011375.1Lag0011375.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding