Lag0010515 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0010515
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA-binding storekeeper protein-related transcriptional regulator
Locationchr1: 331783 .. 332331 (-)
RNA-Seq ExpressionLag0010515
SyntenyLag0010515
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAGAACATATTCATCCCCATGGAACAAAGAGGATGAGTTAATTGTCCTTGAAAAGCTATTTGAATTTTCAACTAATTATTACACGTCCGAATTCTACAACTATGTCAATAAAATGCTAAGTGTTCAACTCTCTGAGAATCAATTGTCTTACATGATATATTGTTTAAGAAAACAATATGTAGAACGTAAAGATGAAAATGGTTTGGATTCTCATGGCCTCAGAGTTTTGGAATTATCGAGGAAAATATGGGAAATTGATAGCAATGAGGGGTGGCCAAAGACAAAAGTACTAAGAGAACATTATGAGAAATATTTACGAATTTATGTTGTTGAAGAATTAGGGTTTTCGACGGATTGCTTGACTGCTTCTGTTTTCGAGGAAATTAAGAAAGATTGGCAAGAGTTTCGATTTGTTCAAGATGAGTTTCGGATGATGCAACGCAGATTTTCGTTGCAGCTTTGTGAATTGAAGACCAACATGCATAATACACAACTTCAACTTGGACACACGTTTGGAAGAAAAAAAGTTCCACAACATCTTTAA

mRNA sequence

ATGCTGAGAACATATTCATCCCCATGGAACAAAGAGGATGAGTTAATTGTCCTTGAAAAGCTATTTGAATTTTCAACTAATTATTACACGTCCGAATTCTACAACTATGTCAATAAAATGCTAAGTGTTCAACTCTCTGAGAATCAATTGTCTTACATGATATATTGTTTAAGAAAACAATATGTAGAACGTAAAGATGAAAATGGTTTGGATTCTCATGGCCTCAGAGTTTTGGAATTATCGAGGAAAATATGGGAAATTGATAGCAATGAGGGGTGGCCAAAGACAAAAGTACTAAGAGAACATTATGAGAAATATTTACGAATTTATGTTGTTGAAGAATTAGGGTTTTCGACGGATTGCTTGACTGCTTCTGTTTTCGAGGAAATTAAGAAAGATTGGCAAGAGTTTCGATTTGTTCAAGATGAGTTTCGGATGATGCAACGCAGATTTTCGTTGCAGCTTTGTGAATTGAAGACCAACATGCATAATACACAACTTCAACTTGGACACACGTTTGGAAGAAAAAAAGTTCCACAACATCTTTAA

Coding sequence (CDS)

ATGCTGAGAACATATTCATCCCCATGGAACAAAGAGGATGAGTTAATTGTCCTTGAAAAGCTATTTGAATTTTCAACTAATTATTACACGTCCGAATTCTACAACTATGTCAATAAAATGCTAAGTGTTCAACTCTCTGAGAATCAATTGTCTTACATGATATATTGTTTAAGAAAACAATATGTAGAACGTAAAGATGAAAATGGTTTGGATTCTCATGGCCTCAGAGTTTTGGAATTATCGAGGAAAATATGGGAAATTGATAGCAATGAGGGGTGGCCAAAGACAAAAGTACTAAGAGAACATTATGAGAAATATTTACGAATTTATGTTGTTGAAGAATTAGGGTTTTCGACGGATTGCTTGACTGCTTCTGTTTTCGAGGAAATTAAGAAAGATTGGCAAGAGTTTCGATTTGTTCAAGATGAGTTTCGGATGATGCAACGCAGATTTTCGTTGCAGCTTTGTGAATTGAAGACCAACATGCATAATACACAACTTCAACTTGGACACACGTTTGGAAGAAAAAAAGTTCCACAACATCTTTAA

Protein sequence

MLRTYSSPWNKEDELIVLEKLFEFSTNYYTSEFYNYVNKMLSVQLSENQLSYMIYCLRKQYVERKDENGLDSHGLRVLELSRKIWEIDSNEGWPKTKVLREHYEKYLRIYVVEELGFSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCELKTNMHNTQLQLGHTFGRKKVPQHL
Homology
BLAST of Lag0010515 vs. NCBI nr
Match: KAG6607616.1 (hypothetical protein SDJN03_00958, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 186.4 bits (472), Expect = 2.2e-43
Identity = 97/172 (56.40%), Postives = 128/172 (74.42%), Query Frame = 0

Query: 3   RTYSSPWNKEDELIVLEKLFEF-----STNYYTSEFYNYVNKMLSVQLSENQLSYMIYCL 62
           + YS  W+K DE+IVLEKL EF     S NYYTS+FYN V+K LSV + +NQL YMIYCL
Sbjct: 20  KQYSLGWSKRDEVIVLEKLCEFEGQHKSANYYTSQFYNSVHKKLSVDIPQNQLCYMIYCL 79

Query: 63  RKQYVERKDENGLDSHGLRVLELSRKIWEIDSNEGWPKTKVLR-EHYEKYLRIYVVEELG 122
           RKQY+E  +E   D   L+V+ELS K+WE+D+     KTKV+  ++Y ++L++YVV+ LG
Sbjct: 80  RKQYLE--NEKNSDRDDLKVVELSAKLWEVDT-----KTKVVSDDYYLEHLKVYVVQVLG 139

Query: 123 FSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCELKTNMHNTQLQ 169
              DCLT+ V E+IK+DW+EFR V+DEFR +Q RF+LQLCEL  +MHNTQL+
Sbjct: 140 IGADCLTSCVVEKIKEDWKEFRLVEDEFRSIQARFNLQLCELAGDMHNTQLE 184

BLAST of Lag0010515 vs. NCBI nr
Match: XP_022158994.1 (probable transcription factor At4g00610 [Momordica charantia])

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-11
Identity = 58/173 (33.53%), Postives = 86/173 (49.71%), Query Frame = 0

Query: 6   SSPWNKEDELIVLEKLFEFS--TNYYTSEFYNYVNKMLSVQLSENQLSYMIYCLRKQYVE 65
           S  W +EDE+ VL++L+EFS     Y+ EFY +V K L  Q+S +Q+   I  LR++Y+E
Sbjct: 55  SFKWREEDEVFVLKQLYEFSGKNATYSEEFYEFVKKKLGGQVSMHQMRNKIRGLRRKYLE 114

Query: 66  RKDENGL-------DSHGLRVLELSRKIWEIDSNEGWPKTK--VLREHYEKYLRIYV--V 125
             + NGL         H  +VL  S++IW    NE   K K        E+Y R +V  +
Sbjct: 115 NDECNGLKFCGSGSQQHQRKVLAWSKRIWGAAENENENKNKNGGGTRSVEQYERGFVESL 174

Query: 126 EELGFSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCELKTNMHNT 166
           EE+    DCL  S  E + KDW+    +  +    + +   +L EL     NT
Sbjct: 175 EEMEIEMDCLKPSCLEFLYKDWEIQTLMHVQLLSNKCKLDAKLTELCIQFLNT 227

BLAST of Lag0010515 vs. NCBI nr
Match: XP_022158998.1 (probable transcription factor At4g01260 [Momordica charantia])

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-10
Identity = 51/178 (28.65%), Postives = 88/178 (49.44%), Query Frame = 0

Query: 9   WNKEDELIVLEKLFEFS--TNYYTSEFYNYVNKMLSVQLSENQLSYMIYCLRKQYVERKD 68
           W+  DE ++L+  +EF+     Y+ +FY++V   LSV +S +QLS  +Y LRK+++  KD
Sbjct: 64  WSWSDECVILKNFYEFAGKNGSYSKDFYHFVKNKLSVDVSNSQLSDKVYRLRKRFL--KD 123

Query: 69  E------------NGLDSHGLRVLELSRKIWEIDSN--------EGWPKTKVLREHYEKY 128
           E            N L+    ++ E+S  IW  +SN          W    V R   E +
Sbjct: 124 ECKGKIRMKFSKGNQLE----KLYEMSNNIWGSNSNTQNTQSGGNQWKAEAVNRLCEEMF 183

Query: 129 LRIYVVEELGFSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCELKTNMHN 165
            R  V+E +     C+  +V  +I+KDW++ +  QDE+ +      LQ+  ++   +N
Sbjct: 184 ER--VIENVKVDISCIPPNVLXDIRKDWEQMKMAQDEYELKNANLLLQISHIRCTQNN 233

BLAST of Lag0010515 vs. NCBI nr
Match: KAG6585873.1 (putative transcription factor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 77.0 bits (188), Expect = 1.8e-10
Identity = 62/171 (36.26%), Postives = 89/171 (52.05%), Query Frame = 0

Query: 9   WNKEDELIVLEKLFEF--STNYYTSEFYNYVNKML-SVQLSENQLSYMIYCLRKQYVE-- 68
           W +EDE+IVL++L++F  S   Y+  FY +V K L + Q+S +QLS  I   +K+Y++  
Sbjct: 55  WEEEDEIIVLQQLYQFGGSNASYSKAFYEFVKKRLNNNQVSMHQLSNKIDRFKKEYLKNE 114

Query: 69  -RKDENGL---DSHGLRVLELSRKIW---EIDSNEGWPKTKVLREHYEKYLRIYVVEELG 128
            RK EN L   + H   V  LSR+IW      SN G       R  Y K    Y +EE+ 
Sbjct: 115 CRKKENDLKFSEYHEFEVFMLSRRIWGGKRSKSNGG-------RMKYAKGFLEY-LEEMD 174

Query: 129 FSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCEL---KTNMHN 165
              DCLT S  E  KK+W+    +  E    + +F  +L EL   K++ HN
Sbjct: 175 IDIDCLTPSCLEHFKKEWEVQLLMYIELLSKKSKFDAKLMELLFTKSSHHN 217

BLAST of Lag0010515 vs. NCBI nr
Match: XP_022951915.1 (probable transcription factor At1g66420 [Cucurbita moschata])

HSP 1 Score: 75.5 bits (184), Expect = 5.4e-10
Identity = 59/162 (36.42%), Postives = 84/162 (51.85%), Query Frame = 0

Query: 9   WNKEDELIVLEKLFEF--STNYYTSEFYNYVNKML-SVQLSENQLSYMIYCLRKQYVE-- 68
           W +EDE+IVL++L++F  S   Y+  FY +V K L + Q+S +QLS  I   +K+Y++  
Sbjct: 55  WEEEDEIIVLQQLYQFGGSNASYSKAFYEFVKKRLNNNQVSMHQLSNKIDRFKKEYLKNE 114

Query: 69  -RKDENGL---DSHGLRVLELSRKIW---EIDSNEGWPKTKVLREHYEKYLRIYVVEELG 128
            RK EN L   + H   V  LSR+IW      SN G       R  Y K    Y +EE+ 
Sbjct: 115 CRKKENDLKFSEYHEFEVFMLSRRIWGGKRSKSNGG-------RMKYAKGFLEY-LEEMD 174

Query: 129 FSTDCLTASVFEEIKKDWQEFRFVQDEFRMMQRRFSLQLCEL 159
              DCLT S  E  KK+W+    +  E    + +F  +L EL
Sbjct: 175 IDIDCLTPSCLEHFKKEWEVQLLMYIELLSKKSKFDAKLMEL 208

BLAST of Lag0010515 vs. ExPASy Swiss-Prot
Match: Q9SYA9 (Probable transcription factor At1g61730 OS=Arabidopsis thaliana OX=3702 GN=At1g61730 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 6.4e-06
Identity = 31/96 (32.29%), Postives = 55/96 (57.29%), Query Frame = 0

Query: 9   WNKEDELIVLEKLFEF------STNYYTSEFYNYVNKMLSVQLSENQLSYMIYCLRKQYV 68
           W++EDE++VL+ + +F      S    T+ FY+++ K +S ++S+NQ    I  LRK+Y+
Sbjct: 159 WSEEDEILVLQGMIDFKADTGKSPYVDTNAFYDFLKKSISFEVSKNQFMDKIRSLRKKYI 218

Query: 69  ERKDENG---LDSHGLRVLELSRKIW-----EIDSN 91
            ++  N    + +H  +  ELS+ IW      +DSN
Sbjct: 219 GKEGRNEPSFVKAHDKKAFELSKFIWGPKGIALDSN 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6607616.12.2e-4356.40hypothetical protein SDJN03_00958, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022158994.13.7e-1133.53probable transcription factor At4g00610 [Momordica charantia][more]
XP_022158998.11.4e-1028.65probable transcription factor At4g01260 [Momordica charantia][more]
KAG6585873.11.8e-1036.26putative transcription factor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022951915.15.4e-1036.42probable transcription factor At1g66420 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SYA96.4e-0632.29Probable transcription factor At1g61730 OS=Arabidopsis thaliana OX=3702 GN=At1g6... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007592GLABROUS1 enhancer-binding protein familyPFAMPF04504DUF573coord: 9..86
e-value: 3.7E-10
score: 40.3
IPR007592GLABROUS1 enhancer-binding protein familyPANTHERPTHR31662BNAANNG10740D PROTEIN-RELATEDcoord: 7..148
NoneNo IPR availablePANTHERPTHR31662:SF33STOREKEEPER PROTEINcoord: 7..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0010515.1Lag0010515.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0005488 binding