Lag0010420 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0010420
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein kinase domain-containing protein
Locationchr9: 47120597 .. 47123739 (+)
RNA-Seq ExpressionLag0010420
SyntenyLag0010420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAATCTGCTGCGCAAAACCTGCCAAGCTTGCTCATGCTTCTTCCAGCAATATTTCTGGTAATCTATCTATCTCTCATCTCTGCTTCTTCGTTTAATTAGTTTTATTTTTCAGAACCTGGCCGCGATCAGTAGATCACTGAGTGTTGGTTGTGGTTTTTTTACGAACTATTAGCTCCAGAGTGAATCGGTTATTATTAATTGGAAAAGCAATGTGAAAGGAAGCAATTCTCTATAGCAACTACGTTTGTCAATTTTCAGATCTCCATGCCGTTGTAATTAATTCGTAAACCTTGCTGCTGTTTGGTTACTTGCTGGCTGCGCATACATTTGCCATTTATAACGTTGAGGTTCTGGCATATGTTGGGTATTGTGTTATTTTGATGTGAAAGCCAAGTCGTAAATATTATGATCAGGACATTTTCGATAAAAGAAAGTCCGGGGATTGCAGATTTTAGCCAAATTTATTGTTTTTTGTTTTTGGATTTCCTCAATTCTTTTGTTTTCCTGGCAGGAAGGGATAACCCAAATAGCAAGTCGAACTTGGAATCAACTGATTCTAGTCTGAAAACTGTTAGCCTTACCTCTAATACGCCTGTCATCAAACCGAAATTCGATGTATCTGGCGCCTGTGCTGTCAAGTCTTTTAGCTTCATTGATCTCAAGAATGCCACGAAGAACTTCCGCAGTGAAAGTCTACTTGGTGAGGGAGGATTTGGATGCGTCTTTAAAGGATGGATCGATGAGCACTCTTATCTGCCCACTAAACCCGGAACTGGAATTGTAGTGGCTGTCAAGAAACTCAAGCGAGAGAGCCTTCAAGGCTACAAGGAATGGCTTGTACGTATACTATGAACTCTGCCATCTCTCATAATCTGTTTTCTGACTAATTTACCTTTAACTTCGTTTGAATCTCTACGTTTCAGGCAGAAGTAAACTATCTGGGTCAGCTTCGCCATGAAAATCTTGTCAGGCTCATTGGGTATTGCTCAGAATCTGATAACAGACTTCTAGTTTATGAGTTTATGCCGAAGGGAAGTCTGGAAAATCATTTGTTTAGAAGTTAGTATGCCACCCCATCATTACCTTTGTTCTTTATTCAACAACGTTCTTCTCTTGCTCAAGTTTAAAGCTATTTGCTTGCTTCTATTCGTATCTAGATTAGTGCTTTTCTCACCTCCATTTTTTAGTGCTTTAACTCTAGCATTTTAAAAAGTAAAAATGGTATGAGAAATTCAATAAAAGATACAAGCGACATGGAAGCATTGTTTATCCAGAGAGTATTGTTTAATGAAGTAACTTTTTATTGATCATCAGTGATCAAGGAGCTGGGCTTTTAAAGTGGCAACATCTAGGTGGCCTTTAGGCTGTTAATAACTAGCAAACAGTTGGTATCTCTTTAACCAGTTACCAGGGGAAGTTATATGGTAAATAACTGTCACCAACTTAATTTGTTTAATTTGCTTTTGTTATAAAACGGCGAAACTATGCTGTTCGCAATAAAATTGAGAGAGCCAAAAGCAGATGGGAGTAGATGGAAACTTTTTTCTATTGTCATTGGATAATAAATTGTTACTCAAGTAGCCCAAGTTTTTAGGAACTGATGCTTTCGAAATGGACGCATTTAAAGGAAGCTCCTTATACAATATATATGTTTTTTCTTTTGTAAGTACTTAAATTATTTCTTATAGAAAGAGCATACGTGCTTGGATGTTCTATTTACATTGCATGTTCAGATTGGTAACGAAAGAAAACGGTTATTTAGTCTGATGTTGGATTTTTTTTTTTTCTTCTTCTTTTCGTTTTCATTAAAGCATTTACAAATATGAAACTCACATCGGAGACTGGATACAAGTCTTCCTGGTACTTTTAAGGGAGTCAGAATGATGGTAGCAAATATGAAACTCACATCGGAAACTCACACTATGAGTTTTTGCTTTGGAGAAGGACAAGCATTTGCATGATAATCTTTAAATTTTTAAAAAGTTTCATGTGCTGCAGTATTTTTCCCTGGGAATTCGTAATGATTCTTATCGTTCTGTGTCTTTACAGAAGGTGTTACACCTATTTCTTGGCGTGTTCGGATGAATATTGCAGTTGATGTGGCACGGGGATTATCATTCTTACACAGTTTTGAACCTAATGTTATATATCGTGATTTGAAGGCTTCCAACATCCTGCTGGATTCGGTGAGTAATTTTTTTGTCCAATCAAGGGAAAGAAAATCCCCTACGAAAATACTCATCTCTCTCTGTTTCTTTCTTTTAAGGAATTCAATGCAAAACTCTCAGATTTTGGGTTAGCGAGGGAGGGTCCTACGGGGGACAAGACTCATGTTTCAACTCGGGTAATGGGAACTCGTGGCTATGCTGCCCCAGAATATGTAGCAACAGGTTGGTTAGATTTCATTTATTTAATTCTTTAACGTTTCTTGATTGACTAGGCATGTCAAGTAAAAGCAAATTTTGAATCTCAAAAAGTAACAATCTATTTCGTTCTTAGCTCATGTACGGGATGTTTGAATTCATTGTCTCTTTCTTTTTAAGATTTGGTGTATTGAACTAAAGCTCAAGAGGAAGATTGTTCTATACTACATTATGACCATCTGAAGTTTAAGACGTTTGAAATGCAGCCACATCAGCGAATATACTTTTCTTTTTTTTTTCAATATAGAACAAAGATGATTCTTGTATAGGAAAGGATACATTATAGGGATACCGCACTCTTCTCTTACTCTGCTTTGGTGATTGGTTTGATTGACTAAACAGGTCACTTGACTCCAAAGAGTGATGTATACAGCTTTGGCGTCGTTTTGCTAGAATTGCTCTCAGGCAAACGTGCATTGGACCAGGAGAAAGTTGGGAGAGTGGAAGAGACCCTAGTGGATTGGGGGAAACCCCTTCTAAGTGACAGCAAGCGAATGTTGAGGATAATGGATACGAGGCTAGGAGGTCAATATTCAAGAAAGGAAGCTCAAGCTGCAGCTTCGATTGCACTGAATTGTCTCCACACGGATCCCAAAAACAGGCCATCCATGGTTGATGTTCTGGACGAATTAGAACGACTACACACCGCAAAGGATGTTCTAAGGACCCCAGACGGCCATGCCATCAGACGCACGCCACCTGCTAGACCTCAATAG

mRNA sequence

ATGGGAATCTGCTGCGCAAAACCTGCCAAGCTTGCTCATGCTTCTTCCAGCAATATTTCTGGAAGGGATAACCCAAATAGCAAGTCGAACTTGGAATCAACTGATTCTAGTCTGAAAACTGTTAGCCTTACCTCTAATACGCCTGTCATCAAACCGAAATTCGATGTATCTGGCGCCTGTGCTGTCAAGTCTTTTAGCTTCATTGATCTCAAGAATGCCACGAAGAACTTCCGCAGTGAAAGTCTACTTGGTGAGGGAGGATTTGGATGCGTCTTTAAAGGATGGATCGATGAGCACTCTTATCTGCCCACTAAACCCGGAACTGGAATTGTAGTGGCTGTCAAGAAACTCAAGCGAGAGAGCCTTCAAGGCTACAAGGAATGGCTTGCAGAAGTAAACTATCTGGGTCAGCTTCGCCATGAAAATCTTGTCAGGCTCATTGGGTATTGCTCAGAATCTGATAACAGACTTCTAGTTTATGAGTTTATGCCGAAGGGAAGTCTGGAAAATCATTTGTTTAGAAAAGGTGTTACACCTATTTCTTGGCGTGTTCGGATGAATATTGCAGTTGATGTGGCACGGGGATTATCATTCTTACACAGTTTTGAACCTAATGTTATATATCGTGATTTGAAGGCTTCCAACATCCTGCTGGATTCGGAATTCAATGCAAAACTCTCAGATTTTGGGTTAGCGAGGGAGGGTCCTACGGGGGACAAGACTCATGTTTCAACTCGGGTAATGGGAACTCGTGGCTATGCTGCCCCAGAATATGTAGCAACAGGTCACTTGACTCCAAAGAGTGATGTATACAGCTTTGGCGTCGTTTTGCTAGAATTGCTCTCAGGCAAACGTGCATTGGACCAGGAGAAAGTTGGGAGAGTGGAAGAGACCCTAGTGGATTGGGGGAAACCCCTTCTAAGTGACAGCAAGCGAATGTTGAGGATAATGGATACGAGGCTAGGAGGTCAATATTCAAGAAAGGAAGCTCAAGCTGCAGCTTCGATTGCACTGAATTGTCTCCACACGGATCCCAAAAACAGGCCATCCATGGTTGATGTTCTGGACGAATTAGAACGACTACACACCGCAAAGGATGTTCTAAGGACCCCAGACGGCCATGCCATCAGACGCACGCCACCTGCTAGACCTCAATAG

Coding sequence (CDS)

ATGGGAATCTGCTGCGCAAAACCTGCCAAGCTTGCTCATGCTTCTTCCAGCAATATTTCTGGAAGGGATAACCCAAATAGCAAGTCGAACTTGGAATCAACTGATTCTAGTCTGAAAACTGTTAGCCTTACCTCTAATACGCCTGTCATCAAACCGAAATTCGATGTATCTGGCGCCTGTGCTGTCAAGTCTTTTAGCTTCATTGATCTCAAGAATGCCACGAAGAACTTCCGCAGTGAAAGTCTACTTGGTGAGGGAGGATTTGGATGCGTCTTTAAAGGATGGATCGATGAGCACTCTTATCTGCCCACTAAACCCGGAACTGGAATTGTAGTGGCTGTCAAGAAACTCAAGCGAGAGAGCCTTCAAGGCTACAAGGAATGGCTTGCAGAAGTAAACTATCTGGGTCAGCTTCGCCATGAAAATCTTGTCAGGCTCATTGGGTATTGCTCAGAATCTGATAACAGACTTCTAGTTTATGAGTTTATGCCGAAGGGAAGTCTGGAAAATCATTTGTTTAGAAAAGGTGTTACACCTATTTCTTGGCGTGTTCGGATGAATATTGCAGTTGATGTGGCACGGGGATTATCATTCTTACACAGTTTTGAACCTAATGTTATATATCGTGATTTGAAGGCTTCCAACATCCTGCTGGATTCGGAATTCAATGCAAAACTCTCAGATTTTGGGTTAGCGAGGGAGGGTCCTACGGGGGACAAGACTCATGTTTCAACTCGGGTAATGGGAACTCGTGGCTATGCTGCCCCAGAATATGTAGCAACAGGTCACTTGACTCCAAAGAGTGATGTATACAGCTTTGGCGTCGTTTTGCTAGAATTGCTCTCAGGCAAACGTGCATTGGACCAGGAGAAAGTTGGGAGAGTGGAAGAGACCCTAGTGGATTGGGGGAAACCCCTTCTAAGTGACAGCAAGCGAATGTTGAGGATAATGGATACGAGGCTAGGAGGTCAATATTCAAGAAAGGAAGCTCAAGCTGCAGCTTCGATTGCACTGAATTGTCTCCACACGGATCCCAAAAACAGGCCATCCATGGTTGATGTTCTGGACGAATTAGAACGACTACACACCGCAAAGGATGTTCTAAGGACCCCAGACGGCCATGCCATCAGACGCACGCCACCTGCTAGACCTCAATAG

Protein sequence

MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELERLHTAKDVLRTPDGHAIRRTPPARPQ
Homology
BLAST of Lag0010420 vs. NCBI nr
Match: KAG6605855.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035804.1 putative serine/threonine-protein kinase PBL3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 718.8 bits (1854), Expect = 2.5e-203
Identity = 358/383 (93.47%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+ STDSS KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGSTDSSQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. NCBI nr
Match: XP_023533194.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 717.6 bits (1851), Expect = 5.7e-203
Identity = 357/383 (93.21%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+ STDS+ KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGSTDSNQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. NCBI nr
Match: XP_022957667.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 716.8 bits (1849), Expect = 9.7e-203
Identity = 357/383 (93.21%), Postives = 371/383 (96.87%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+  TDSS KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGPTDSSQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. NCBI nr
Match: XP_022995417.1 (probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita maxima] >XP_022995418.1 probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 716.5 bits (1848), Expect = 1.3e-202
Identity = 356/383 (92.95%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+ STDSS KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGSTDSSQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEH+YLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHTYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS +PNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCQPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. NCBI nr
Match: XP_011653538.1 (probable serine/threonine-protein kinase PBL3 [Cucumis sativus] >KGN54046.1 hypothetical protein Csa_021645 [Cucumis sativus])

HSP 1 Score: 713.0 bits (1839), Expect = 1.4e-201
Identity = 354/385 (91.95%), Postives = 369/385 (95.84%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASSS ISG DN NSKSN+ES + + KTV+ TS+ PVIKPKFDVSGA 
Sbjct: 1   MGICCAKPAKLAHASSSIISGTDNKNSKSNVESINCNSKTVNFTSSAPVIKPKFDVSGAS 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE
Sbjct: 61  ALKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYE+MPKGSLENHLFRKGVTPI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEYMPKGSLENHLFRKGVTPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRM+IAVDVARGL+FLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALD EKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDHEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSD KRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSM +VLDELER
Sbjct: 301 DWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMAEVLDELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPARPQ 386
           LHTAKD+L TP+ HAIRRTPP+R Q
Sbjct: 361 LHTAKDILGTPNAHAIRRTPPSRFQ 385

BLAST of Lag0010420 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 3.2e-124
Identity = 221/371 (59.57%), Postives = 282/371 (76.01%), Query Frame = 0

Query: 1   MGICCAKPAKL------AHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKF 60
           MG C    AK+       HA+S+++S R +  +  +   +  S+ + S   + P  + + 
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEG 60

Query: 61  DVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAV 120
           ++  +  +K+F+F +LKNAT+NFR +SLLGEGGFG VFKGWID  +   +KPG+GIVVAV
Sbjct: 61  EILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAV 120

Query: 121 KKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFR 180
           KKLK E  QG+KEWL EVNYLGQL H NLV+L+GYC E +NRLLVYEFMPKGSLENHLFR
Sbjct: 121 KKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFR 180

Query: 181 KGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLARE 240
           +G  P++W +RM +A+  A+GL+FLH  +  VIYRD KA+NILLD+EFN+KLSDFGLA+ 
Sbjct: 181 RGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKA 240

Query: 241 GPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGR 300
           GPTGDKTHVST+VMGT GYAAPEYVATG LT KSDVYSFGVVLLELLSG+RA+D+ KVG 
Sbjct: 241 GPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVG- 300

Query: 301 VEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDV 360
           +E++LVDW  P L D +++ RIMDTRLGGQY +K A  AAS+AL CL+ D K RP M +V
Sbjct: 301 MEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLALQCLNPDAKLRPKMSEV 360

Query: 361 LDELERLHTAK 366
           L +L++L + K
Sbjct: 361 LAKLDQLESTK 370

BLAST of Lag0010420 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.7e-120
Identity = 216/372 (58.06%), Postives = 279/372 (75.00%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSS----NISGRDNPNSKSNLESTDSSLKTVSLTSNT-----PVIK 60
           MG C    AK+ +++ S    + S     +SK++  +  S L T S ++++     P ++
Sbjct: 1   MGNCLDSSAKVDNSNHSPHANSASSGSKVSSKTSRSTGPSGLSTTSYSTDSSFGPLPTLR 60

Query: 61  PKFDVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIV 120
            + ++  +  +K+F+F +LKNATKNFR ++LLGEGGFGCVFKGWID+ S   ++PG+GIV
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGIV 120

Query: 121 VAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENH 180
           VAVK+LK E  QG+KEWL EVNYLGQL H NLV L+GYC+E +NRLLVYEFMPKGSLENH
Sbjct: 121 VAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLLVGYCAEGENRLLVYEFMPKGSLENH 180

Query: 181 LFRKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGL 240
           LFR+G  P++W +RM +AV  A+GL+FLH  +  VIYRD KA+NILLD++FNAKLSDFGL
Sbjct: 181 LFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDADFNAKLSDFGL 240

Query: 241 AREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEK 300
           A+ GPTGD THVST+V+GT GYAAPEYVATG LT KSDVYSFGVVLLEL+SG+RA+D   
Sbjct: 241 AKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNSN 300

Query: 301 VGRVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSM 360
            G  E +LVDW  P L D +++ RIMDT+LGGQY +K A  AA++AL CL+ D K RP M
Sbjct: 301 GGN-EYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKM 360

Query: 361 VDVLDELERLHT 364
            +VL  LE+L +
Sbjct: 361 SEVLVTLEQLES 371

BLAST of Lag0010420 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.8e-119
Identity = 225/392 (57.40%), Postives = 285/392 (72.70%), Query Frame = 0

Query: 1   MGICCAKPAKLAH-----ASSSNISGRDNPNSKSNLES------TDSSLKTVSLTSNTPV 60
           MG C    A++ +       SS IS +  PN  S L S      +++S  T S ++ TP 
Sbjct: 1   MGNCLDSSARVGNRESTFGGSSRISRK--PNQSSRLSSLTIPSYSNNSFTTSSWSNLTP- 60

Query: 61  IKPKFDVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTG 120
            + + ++  +  +K+F+F +LK AT+NF+  S++GEGGFGCV+KGWI E S  P+KPG+G
Sbjct: 61  -RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSG 120

Query: 121 IVVAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLE 180
           +VVAVKKLK E  QG+KEWL EV+YLG+L H NLV+LIGYC E + RLLVYE+MPKGSLE
Sbjct: 121 MVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLE 180

Query: 181 NHLFRKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDF 240
           NHLFR+G  PI W+ RM +A   ARGLSFLH  E  VIYRD KASNILLD +FNAKLSDF
Sbjct: 181 NHLFRRGAEPIPWKTRMKVAFSAARGLSFLH--EAKVIYRDFKASNILLDVDFNAKLSDF 240

Query: 241 GLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQ 300
           GLA+ GPTGD+THV+T+V+GT+GYAAPEY+ATG LT KSDVYSFGVVLLELLSG+  LD+
Sbjct: 241 GLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSKSDVYSFGVVLLELLSGRPTLDK 300

Query: 301 EKVGRVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRP 360
            KVG VE  LVDW  P L D +++ RIMDT+LGGQY  K A AAA+IAL CL+T+PK RP
Sbjct: 301 SKVG-VERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHKGACAAANIALRCLNTEPKLRP 360

Query: 361 SMVDVLDELERLHTA-------KDVLRTPDGH 375
            M DVL  L++L T+       ++++ +P  H
Sbjct: 361 DMADVLSTLQQLETSSKKMGSTQNIVMSPSSH 385

BLAST of Lag0010420 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.6e-115
Identity = 216/368 (58.70%), Postives = 273/368 (74.18%), Query Frame = 0

Query: 1   MGICCAKPAKLA-----HASSSNISGRDNPNSK-SNLESTDSSLKTVSLTSNTPVIKPKF 60
           MG C    AK+      +  SS IS + + +S+ S+L    SS    +  ++    + + 
Sbjct: 1   MGNCFGFSAKVGNRESPYRGSSRISAKRSQSSRLSSLTIQSSSYNDDTSVASLQTPRSEG 60

Query: 61  DVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAV 120
           ++  +  +K+F+F +LK AT+NFR +S++GEGGFG V+KGWIDE +  P+KPG+G+VVAV
Sbjct: 61  ELLASPTLKAFTFNELKTATRNFRPDSVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAV 120

Query: 121 KKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDN-RLLVYEFMPKGSLENHLF 180
           KKLK E  QG+++WLAEV+ LG+L H NLV+LIGYCS+ D+ RLLVYE+MPKGSLENHLF
Sbjct: 121 KKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIGYCSKGDHIRLLVYEYMPKGSLENHLF 180

Query: 181 RKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAR 240
           R+G  PI WR R+ +A+  ARGL+FLH  E  VIYRD KASNILLDSEFNAKLSDFGLA+
Sbjct: 181 RRGAEPIPWRTRIKVAIGAARGLAFLH--EAQVIYRDFKASNILLDSEFNAKLSDFGLAK 240

Query: 241 EGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVG 300
            GPTGD+THVST+VMGT+GYAAPEYVATG +T KSDVYSFGVVLLELLSG+  +D+ KVG
Sbjct: 241 VGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVG 300

Query: 301 RVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVD 360
            VE  LVDW  P L D +++ RIMDT+LGGQY  K A   A+ AL CL+ +PK RP M D
Sbjct: 301 -VERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHKGACLTANTALQCLNQEPKLRPKMSD 360

Query: 361 VLDELERL 362
           VL  LE L
Sbjct: 361 VLSTLEEL 365

BLAST of Lag0010420 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 4.6e-115
Identity = 210/360 (58.33%), Postives = 269/360 (74.72%), Query Frame = 0

Query: 14  ASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGACAVKSFSFIDLKNA 73
           A  S+ S   + +S S   S  +S  +VS  S  P  + + ++  A  VK+F+F +L+ A
Sbjct: 7   AKISSESPCRSASSPSGGTSKYASNSSVSAASVPPTPRSEDEILEAANVKAFAFNELRTA 66

Query: 74  TKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRESLQGYKEWLAEVN 133
           T+NFR +S+LGEGGFG VFKGWIDE +  PTKPGTG+V+AVKKL +E  QG++EWLAEVN
Sbjct: 67  TRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVN 126

Query: 134 YLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGV--TPISWRVRMNIAVD 193
           YLGQL H  LVRL+GYC E + RLLVYEFMP+GSLENHLFR+     P+SW +RM IA+ 
Sbjct: 127 YLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHFQPLSWNLRMKIALG 186

Query: 194 VARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDKTHVSTRVMGTR 253
            A+GL+FLHS +  VIYRD K SN+LLD+ ++AKLSDFGLA++GPTGDK+HVSTRVMGT 
Sbjct: 187 AAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRVMGTY 246

Query: 254 GYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLVDWGKPLLSDSK 313
           GYAAPEY+ATGHLT KSDVYSFGVVLLE+LSG+RALD+ +    E  LV+W +P L   +
Sbjct: 247 GYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNR-PTGEHNLVEWARPYLMSKR 306

Query: 314 RMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELERLHTAKDVLRTP 372
           R+ RI+D RLGGQYS  +AQ AA++AL C+  + KNRP+M  V+  LE+L  +K+    P
Sbjct: 307 RIFRILDARLGGQYSLAKAQKAATLALQCISVEAKNRPNMEQVVAVLEQLQDSKETGANP 365

BLAST of Lag0010420 vs. ExPASy TrEMBL
Match: A0A6J1GZR7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459133 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.7e-203
Identity = 357/383 (93.21%), Postives = 371/383 (96.87%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+  TDSS KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGPTDSSQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. ExPASy TrEMBL
Match: A0A6J1K7V7 (probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490965 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.1e-203
Identity = 356/383 (92.95%), Postives = 372/383 (97.13%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASS+NIS RDN NSKSN+ STDSS KT+SLTS+T VIKPKFDVSGAC
Sbjct: 1   MGICCAKPAKLAHASSTNISARDNLNSKSNVGSTDSSQKTLSLTSSTSVIKPKFDVSGAC 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDL+NATKNFRSESLLGEGGFGCVFKGWIDEH+YLPTKPGTGIVVAVKKLKR 
Sbjct: 61  ALKSFSFIDLRNATKNFRSESLLGEGGFGCVFKGWIDEHTYLPTKPGTGIVVAVKKLKRG 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRH+NLVRLIGYCSESD+RLLVYE+MPKGSLENHLFRKGV PI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHDNLVRLIGYCSESDDRLLVYEYMPKGSLENHLFRKGVAPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRMNIAVDVARGLSFLHS +PNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMNIAVDVARGLSFLHSCQPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSDSKRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSMVDVL+ELER
Sbjct: 301 DWGKPLLSDSKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMVDVLEELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPAR 384
           L ++KDVLRTPD  AIRRTPPAR
Sbjct: 361 LLSSKDVLRTPDSRAIRRTPPAR 383

BLAST of Lag0010420 vs. ExPASy TrEMBL
Match: A0A0A0L1P7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G269770 PE=3 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 6.8e-202
Identity = 354/385 (91.95%), Postives = 369/385 (95.84%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASSS ISG DN NSKSN+ES + + KTV+ TS+ PVIKPKFDVSGA 
Sbjct: 1   MGICCAKPAKLAHASSSIISGTDNKNSKSNVESINCNSKTVNFTSSAPVIKPKFDVSGAS 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE
Sbjct: 61  ALKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYE+MPKGSLENHLFRKGVTPI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEYMPKGSLENHLFRKGVTPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRM+IAVDVARGL+FLHS EPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALD EKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDHEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSD KRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSM +VLDELER
Sbjct: 301 DWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMAEVLDELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPPARPQ 386
           LHTAKD+L TP+ HAIRRTPP+R Q
Sbjct: 361 LHTAKDILGTPNAHAIRRTPPSRFQ 385

BLAST of Lag0010420 vs. ExPASy TrEMBL
Match: A0A5A7TEX2 (Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00820 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 9.8e-201
Identity = 353/381 (92.65%), Postives = 365/381 (95.80%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASSS ISG DN NSKS +ES +SS KTV+L S+ PVIKPKFDVSGA 
Sbjct: 1   MGICCAKPAKLAHASSSIISGTDNQNSKSKVESINSSSKTVNLISSVPVIKPKFDVSGAS 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE
Sbjct: 61  ALKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYE+MPKGSLENHLFRKGVTPI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEYMPKGSLENHLFRKGVTPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRM+IAVDVARGL+FLHS  PNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMDIAVDVARGLAFLHSSRPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSD KRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSM +VLDELER
Sbjct: 301 DWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMAEVLDELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPP 382
           LH AKDVL TP+ HAIRRTPP
Sbjct: 361 LHAAKDVLGTPNAHAIRRTPP 381

BLAST of Lag0010420 vs. ExPASy TrEMBL
Match: A0A1S3BNM8 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491609 PE=3 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 9.8e-201
Identity = 353/381 (92.65%), Postives = 365/381 (95.80%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKFDVSGAC 60
           MGICCAKPAKLAHASSS ISG DN NSKS +ES +SS KTV+L S+ PVIKPKFDVSGA 
Sbjct: 1   MGICCAKPAKLAHASSSIISGTDNQNSKSKVESINSSSKTVNLISSVPVIKPKFDVSGAS 60

Query: 61  AVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120
           A+KSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE
Sbjct: 61  ALKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAVKKLKRE 120

Query: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFRKGVTPI 180
           SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYE+MPKGSLENHLFRKGVTPI
Sbjct: 121 SLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEYMPKGSLENHLFRKGVTPI 180

Query: 181 SWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240
           SWRVRM+IAVDVARGL+FLHS  PNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK
Sbjct: 181 SWRVRMDIAVDVARGLAFLHSSRPNVIYRDLKASNILLDSEFNAKLSDFGLAREGPTGDK 240

Query: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300
           THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV
Sbjct: 241 THVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGRVEETLV 300

Query: 301 DWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDVLDELER 360
           DWGKPLLSD KRMLRIMDTR+GGQYSRKEAQAAAS+ALNCLHTDPKNRPSM +VLDELER
Sbjct: 301 DWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLHTDPKNRPSMAEVLDELER 360

Query: 361 LHTAKDVLRTPDGHAIRRTPP 382
           LH AKDVL TP+ HAIRRTPP
Sbjct: 361 LHAAKDVLGTPNAHAIRRTPP 381

BLAST of Lag0010420 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 446.4 bits (1147), Expect = 2.3e-125
Identity = 221/371 (59.57%), Postives = 282/371 (76.01%), Query Frame = 0

Query: 1   MGICCAKPAKL------AHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKF 60
           MG C    AK+       HA+S+++S R +  +  +   +  S+ + S   + P  + + 
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEG 60

Query: 61  DVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAV 120
           ++  +  +K+F+F +LKNAT+NFR +SLLGEGGFG VFKGWID  +   +KPG+GIVVAV
Sbjct: 61  EILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAV 120

Query: 121 KKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFR 180
           KKLK E  QG+KEWL EVNYLGQL H NLV+L+GYC E +NRLLVYEFMPKGSLENHLFR
Sbjct: 121 KKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFR 180

Query: 181 KGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLARE 240
           +G  P++W +RM +A+  A+GL+FLH  +  VIYRD KA+NILLD+EFN+KLSDFGLA+ 
Sbjct: 181 RGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKA 240

Query: 241 GPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGR 300
           GPTGDKTHVST+VMGT GYAAPEYVATG LT KSDVYSFGVVLLELLSG+RA+D+ KVG 
Sbjct: 241 GPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVG- 300

Query: 301 VEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDV 360
           +E++LVDW  P L D +++ RIMDTRLGGQY +K A  AAS+AL CL+ D K RP M +V
Sbjct: 301 MEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLALQCLNPDAKLRPKMSEV 360

Query: 361 LDELERLHTAK 366
           L +L++L + K
Sbjct: 361 LAKLDQLESTK 370

BLAST of Lag0010420 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 446.4 bits (1147), Expect = 2.3e-125
Identity = 221/371 (59.57%), Postives = 282/371 (76.01%), Query Frame = 0

Query: 1   MGICCAKPAKL------AHASSSNISGRDNPNSKSNLESTDSSLKTVSLTSNTPVIKPKF 60
           MG C    AK+       HA+S+++S R +  +  +   +  S+ + S   + P  + + 
Sbjct: 1   MGNCLDSSAKVDSSSHSPHANSASLSSRVSSKTSRSTVPSSLSINSYSSVESLPTPRTEG 60

Query: 61  DVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAV 120
           ++  +  +K+F+F +LKNAT+NFR +SLLGEGGFG VFKGWID  +   +KPG+GIVVAV
Sbjct: 61  EILSSPNLKAFTFNELKNATRNFRPDSLLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAV 120

Query: 121 KKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENHLFR 180
           KKLK E  QG+KEWL EVNYLGQL H NLV+L+GYC E +NRLLVYEFMPKGSLENHLFR
Sbjct: 121 KKLKTEGYQGHKEWLTEVNYLGQLSHPNLVKLVGYCVEGENRLLVYEFMPKGSLENHLFR 180

Query: 181 KGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLARE 240
           +G  P++W +RM +A+  A+GL+FLH  +  VIYRD KA+NILLD+EFN+KLSDFGLA+ 
Sbjct: 181 RGAQPLTWAIRMKVAIGAAKGLTFLHDAKSQVIYRDFKAANILLDAEFNSKLSDFGLAKA 240

Query: 241 GPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVGR 300
           GPTGDKTHVST+VMGT GYAAPEYVATG LT KSDVYSFGVVLLELLSG+RA+D+ KVG 
Sbjct: 241 GPTGDKTHVSTQVMGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELLSGRRAVDKSKVG- 300

Query: 301 VEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVDV 360
           +E++LVDW  P L D +++ RIMDTRLGGQY +K A  AAS+AL CL+ D K RP M +V
Sbjct: 301 MEQSLVDWATPYLGDKRKLFRIMDTRLGGQYPQKGAYTAASLALQCLNPDAKLRPKMSEV 360

Query: 361 LDELERLHTAK 366
           L +L++L + K
Sbjct: 361 LAKLDQLESTK 370

BLAST of Lag0010420 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 433.0 bits (1112), Expect = 2.6e-121
Identity = 216/372 (58.06%), Postives = 279/372 (75.00%), Query Frame = 0

Query: 1   MGICCAKPAKLAHASSS----NISGRDNPNSKSNLESTDSSLKTVSLTSNT-----PVIK 60
           MG C    AK+ +++ S    + S     +SK++  +  S L T S ++++     P ++
Sbjct: 1   MGNCLDSSAKVDNSNHSPHANSASSGSKVSSKTSRSTGPSGLSTTSYSTDSSFGPLPTLR 60

Query: 61  PKFDVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIV 120
            + ++  +  +K+F+F +LKNATKNFR ++LLGEGGFGCVFKGWID+ S   ++PG+GIV
Sbjct: 61  TEGEILSSPNLKAFTFNELKNATKNFRQDNLLGEGGFGCVFKGWIDQTSLTASRPGSGIV 120

Query: 121 VAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLENH 180
           VAVK+LK E  QG+KEWL EVNYLGQL H NLV L+GYC+E +NRLLVYEFMPKGSLENH
Sbjct: 121 VAVKQLKPEGFQGHKEWLTEVNYLGQLSHPNLVLLVGYCAEGENRLLVYEFMPKGSLENH 180

Query: 181 LFRKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGL 240
           LFR+G  P++W +RM +AV  A+GL+FLH  +  VIYRD KA+NILLD++FNAKLSDFGL
Sbjct: 181 LFRRGAQPLTWAIRMKVAVGAAKGLTFLHEAKSQVIYRDFKAANILLDADFNAKLSDFGL 240

Query: 241 AREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEK 300
           A+ GPTGD THVST+V+GT GYAAPEYVATG LT KSDVYSFGVVLLEL+SG+RA+D   
Sbjct: 241 AKAGPTGDNTHVSTKVIGTHGYAAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNSN 300

Query: 301 VGRVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSM 360
            G  E +LVDW  P L D +++ RIMDT+LGGQY +K A  AA++AL CL+ D K RP M
Sbjct: 301 GGN-EYSLVDWATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKM 360

Query: 361 VDVLDELERLHT 364
            +VL  LE+L +
Sbjct: 361 SEVLVTLEQLES 371

BLAST of Lag0010420 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.3e-120
Identity = 225/392 (57.40%), Postives = 285/392 (72.70%), Query Frame = 0

Query: 1   MGICCAKPAKLAH-----ASSSNISGRDNPNSKSNLES------TDSSLKTVSLTSNTPV 60
           MG C    A++ +       SS IS +  PN  S L S      +++S  T S ++ TP 
Sbjct: 1   MGNCLDSSARVGNRESTFGGSSRISRK--PNQSSRLSSLTIPSYSNNSFTTSSWSNLTP- 60

Query: 61  IKPKFDVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTG 120
            + + ++  +  +K+F+F +LK AT+NF+  S++GEGGFGCV+KGWI E S  P+KPG+G
Sbjct: 61  -RSEGELLPSPTLKAFTFNELKTATRNFKPNSMIGEGGFGCVYKGWIGERSLSPSKPGSG 120

Query: 121 IVVAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEFMPKGSLE 180
           +VVAVKKLK E  QG+KEWL EV+YLG+L H NLV+LIGYC E + RLLVYE+MPKGSLE
Sbjct: 121 MVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHMNLVKLIGYCLEGEKRLLVYEYMPKGSLE 180

Query: 181 NHLFRKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDF 240
           NHLFR+G  PI W+ RM +A   ARGLSFLH  E  VIYRD KASNILLD +FNAKLSDF
Sbjct: 181 NHLFRRGAEPIPWKTRMKVAFSAARGLSFLH--EAKVIYRDFKASNILLDVDFNAKLSDF 240

Query: 241 GLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQ 300
           GLA+ GPTGD+THV+T+V+GT+GYAAPEY+ATG LT KSDVYSFGVVLLELLSG+  LD+
Sbjct: 241 GLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGRLTSKSDVYSFGVVLLELLSGRPTLDK 300

Query: 301 EKVGRVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRP 360
            KVG VE  LVDW  P L D +++ RIMDT+LGGQY  K A AAA+IAL CL+T+PK RP
Sbjct: 301 SKVG-VERNLVDWAIPYLVDRRKVFRIMDTKLGGQYPHKGACAAANIALRCLNTEPKLRP 360

Query: 361 SMVDVLDELERLHTA-------KDVLRTPDGH 375
            M DVL  L++L T+       ++++ +P  H
Sbjct: 361 DMADVLSTLQQLETSSKKMGSTQNIVMSPSSH 385

BLAST of Lag0010420 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 416.0 bits (1068), Expect = 3.3e-116
Identity = 216/368 (58.70%), Postives = 273/368 (74.18%), Query Frame = 0

Query: 1   MGICCAKPAKLA-----HASSSNISGRDNPNSK-SNLESTDSSLKTVSLTSNTPVIKPKF 60
           MG C    AK+      +  SS IS + + +S+ S+L    SS    +  ++    + + 
Sbjct: 1   MGNCFGFSAKVGNRESPYRGSSRISAKRSQSSRLSSLTIQSSSYNDDTSVASLQTPRSEG 60

Query: 61  DVSGACAVKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLPTKPGTGIVVAV 120
           ++  +  +K+F+F +LK AT+NFR +S++GEGGFG V+KGWIDE +  P+KPG+G+VVAV
Sbjct: 61  ELLASPTLKAFTFNELKTATRNFRPDSVIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAV 120

Query: 121 KKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDN-RLLVYEFMPKGSLENHLF 180
           KKLK E  QG+++WLAEV+ LG+L H NLV+LIGYCS+ D+ RLLVYE+MPKGSLENHLF
Sbjct: 121 KKLKEEGFQGHRQWLAEVDCLGRLHHMNLVKLIGYCSKGDHIRLLVYEYMPKGSLENHLF 180

Query: 181 RKGVTPISWRVRMNIAVDVARGLSFLHSFEPNVIYRDLKASNILLDSEFNAKLSDFGLAR 240
           R+G  PI WR R+ +A+  ARGL+FLH  E  VIYRD KASNILLDSEFNAKLSDFGLA+
Sbjct: 181 RRGAEPIPWRTRIKVAIGAARGLAFLH--EAQVIYRDFKASNILLDSEFNAKLSDFGLAK 240

Query: 241 EGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGKRALDQEKVG 300
            GPTGD+THVST+VMGT+GYAAPEYVATG +T KSDVYSFGVVLLELLSG+  +D+ KVG
Sbjct: 241 VGPTGDRTHVSTQVMGTQGYAAPEYVATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVG 300

Query: 301 RVEETLVDWGKPLLSDSKRMLRIMDTRLGGQYSRKEAQAAASIALNCLHTDPKNRPSMVD 360
            VE  LVDW  P L D +++ RIMDT+LGGQY  K A   A+ AL CL+ +PK RP M D
Sbjct: 301 -VERNLVDWAIPYLGDKRKVFRIMDTKLGGQYPHKGACLTANTALQCLNQEPKLRPKMSD 360

Query: 361 VLDELERL 362
           VL  LE L
Sbjct: 361 VLSTLEEL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605855.12.5e-20393.47putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s... [more]
XP_023533194.15.7e-20393.21probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita pepo subsp. ... [more]
XP_022957667.19.7e-20393.21probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita moschata][more]
XP_022995417.11.3e-20292.95probable serine/threonine-protein kinase PBL3 isoform X1 [Cucurbita maxima] >XP_... [more]
XP_011653538.11.4e-20191.95probable serine/threonine-protein kinase PBL3 [Cucumis sativus] >KGN54046.1 hypo... [more]
Match NameE-valueIdentityDescription
O498403.2e-12459.57Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN... [more]
O498393.7e-12058.06Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5XF791.8e-11957.40Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G... [more]
Q5PP294.6e-11558.70Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q65XV84.6e-11558.33Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1GZR74.7e-20393.21probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1K7V76.1e-20392.95probable serine/threonine-protein kinase PBL3 isoform X1 OS=Cucurbita maxima OX=... [more]
A0A0A0L1P76.8e-20291.95Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G269... [more]
A0A5A7TEX29.8e-20192.65Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00... [more]
A0A1S3BNM89.8e-20192.65protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491609 PE... [more]
Match NameE-valueIdentityDescription
AT2G02800.12.3e-12559.57protein kinase 2B [more]
AT2G02800.22.3e-12559.57protein kinase 2B [more]
AT1G14370.12.6e-12158.06protein kinase 2A [more]
AT1G69790.11.3e-12057.40Protein kinase superfamily protein [more]
AT1G26970.13.3e-11658.70Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 77..365
e-value: 8.3E-52
score: 188.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 78..356
e-value: 2.8E-50
score: 171.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 77..361
score: 41.445847
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 61..162
e-value: 2.4E-32
score: 113.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 163..373
e-value: 6.1E-64
score: 217.2
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 72..284
e-value: 3.6E-25
score: 86.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..37
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 37..364
NoneNo IPR availablePANTHERPTHR45621:SF111OS07G0686800 PROTEINcoord: 37..364
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 83..361
e-value: 1.04903E-100
score: 297.647
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 83..116
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 206..218
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 55..358

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0010420.1Lag0010420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity