Lag0010395 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0010395 vs. NCBI nr
Match: XP_038900291.1 (non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida])

HSP 1 Score: 274.6 bits (701), Expect = 6.2e-70
Identity = 137/185 (74.05%), Postives = 155/185 (83.78%), Query Frame = 0

Query: 5   SSSSSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDCCSGLK 64
           +S+S+ L   MAL+ VGFVSSNIDQDRAECADQVVGLATCLPYVGG+AKAPTPDCCSGL 
Sbjct: 2   ASTSTHLLIVMALLFVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLN 61

Query: 65  LVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPPNSQEA 124
           LVLQKS+KCLC+LIKDRDDPSLGLKVNLTLALGLP AC +PAD+KDCI LLHL PNS+EA
Sbjct: 62  LVLQKSRKCLCILIKDRDDPSLGLKVNLTLALGLPTACHTPADIKDCIGLLHLSPNSKEA 121

Query: 125 KDFLGFNANANAKGSNASAVPAVS-GGSSPQSPQVKNDGGMRRNQWFGVEIFIWFFISSL 184
           KDFL      N KGSN ++ PA +  G+S Q+ + KNDGG +RNQW GVEIF+WF  SS 
Sbjct: 122 KDFL----ETNPKGSNTTSAPATAVSGNSTQNSEAKNDGGTKRNQWLGVEIFVWFSTSSF 181

Query: 185 LLTCI 189
           LLTCI
Sbjct: 182 LLTCI 182

BLAST of Lag0010395 vs. NCBI nr
Match: XP_023512874.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 137/192 (71.35%), Postives = 159/192 (82.81%), Query Frame = 0

Query: 1   MGSKSSS---SSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTP 60
           M SKSSS   ++ +SCAMAL+LVGFVSSN+DQDRAECADQVVGLATCLPYVGG+A+APTP
Sbjct: 1   MASKSSSTNYTALISCAMALLLVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHL 120
           DCCSGLK+VL+KSKKCLCVLIKDR+DPSLGLKVNLTLALGLPNAC +PA+++DCI LLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 PPNSQEAKDFLGFNANANAKGSNASAVPAVSG-GSSPQSPQVKNDGGMRRNQWFGVEIFI 180
            PNSQ+AKDF+G       KG+N +A PA +  G+S Q+P  K  G   RNQW GVE+FI
Sbjct: 121 SPNSQDAKDFMG------TKGANTTAAPATAAPGNSTQTPVEKGSG--MRNQWLGVEMFI 180

Query: 181 WFFISSLLLTCI 189
            F  SSLL TCI
Sbjct: 181 GFLTSSLLFTCI 184

BLAST of Lag0010395 vs. NCBI nr
Match: XP_022153758.1 (protein YLS3-like [Momordica charantia])

HSP 1 Score: 261.2 bits (666), Expect = 7.1e-66
Identity = 132/188 (70.21%), Postives = 154/188 (81.91%), Query Frame = 0

Query: 1   MGSKSSSSSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDCC 60
           MGSK     AL+ A+ ++LVG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC
Sbjct: 1   MGSK---WCALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCC 60

Query: 61  SGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPPN 120
            GLKLVLQKSKKCLCVLIKDRDDP+LGLKVNLTLALGLP AC +PAD+ +C++LLHLPPN
Sbjct: 61  GGLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPN 120

Query: 121 SQEAKDFLGFNANANAKGSNASAVPAVSGGSSPQSPQVKNDGGMRRNQWFGVEIFIWFFI 180
           SQEAKDFLG   N  A  + ++A  + +   S ++PQ K+DGGMR+ QWFGVEIF+WFF 
Sbjct: 121 SQEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRK-QWFGVEIFLWFFT 180

Query: 181 SSLLLTCI 189
           S LLL CI
Sbjct: 181 SLLLLICI 184

BLAST of Lag0010395 vs. NCBI nr
Match: KAG7010231.1 (Protein YLS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.0 bits (663), Expect = 1.6e-65
Identity = 136/192 (70.83%), Postives = 158/192 (82.29%), Query Frame = 0

Query: 1   MGSKSSS---SSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTP 60
           M SKSSS   ++ +SCAMAL+ VGFVSSN+DQDRAECADQVVGLATCLPYVGG+A+APTP
Sbjct: 1   MASKSSSTNYTALMSCAMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHL 120
           DCCSGLK+VL+KSKKCLCVLIKDR+DPSLGLKVNLTLALGLPNAC +PA+++DCI LLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 PPNSQEAKDFLGFNANANAKGSNASAVPAVSG-GSSPQSPQVKNDGGMRRNQWFGVEIFI 180
            PNSQ+AKDF+G      +KG+N +A PA +  G+S QSP  K  G   RNQW  VE+FI
Sbjct: 121 SPNSQDAKDFMG------SKGANTTAAPATAAPGNSTQSPIEKGSG--MRNQWSSVEMFI 180

Query: 181 WFFISSLLLTCI 189
            F  SSLL TCI
Sbjct: 181 GFLTSSLLFTCI 184

BLAST of Lag0010395 vs. NCBI nr
Match: XP_022986686.1 (protein YLS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 259.2 bits (661), Expect = 2.7e-65
Identity = 133/192 (69.27%), Postives = 158/192 (82.29%), Query Frame = 0

Query: 1   MGSKSSS---SSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTP 60
           M SKSSS   ++ +SCA+AL+ VGFVSSN+DQDRAECADQVVGLATCLPYVGG+A+APTP
Sbjct: 1   MASKSSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTP 60

Query: 61  DCCSGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHL 120
           DCCSGLK+VL+KSKKCLCVLIKDR+DPSLGLKVNLTLALGLPNAC +PA+++DCI LLHL
Sbjct: 61  DCCSGLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHL 120

Query: 121 PPNSQEAKDFLGFNANANAKGSNASAVPAVSG-GSSPQSPQVKNDGGMRRNQWFGVEIFI 180
            PNSQ+AKDF+G      +KG+N +A PA +  G+S Q+P  K  G   RNQW G E+FI
Sbjct: 121 SPNSQDAKDFMG------SKGANTTAAPATAAPGNSTQTPMEKGSG--MRNQWLGAEMFI 180

Query: 181 WFFISSLLLTCI 189
            F  SSLL TC+
Sbjct: 181 AFLTSSLLFTCV 184

BLAST of Lag0010395 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.2e-36
Identity = 90/200 (45.00%), Postives = 121/200 (60.50%), Query Frame = 0

Query: 4   KSSSSSALSCAMALILVGFVSSNID---QDRAECADQVVGLATCLPYVGGDAKAPTPDCC 63
           +S   + ++ A+ALI+V  V +  D   +D+ EC +Q+VG+ATCLPYV G AK+PTPDCC
Sbjct: 2   ESRKINLMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCC 61

Query: 64  SGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPPN 123
           SGLK VL  +KKCLCV+I+DR+DP LGL++N++LAL LP+ C + ADV  C  LLHL PN
Sbjct: 62  SGLKQVLNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPN 121

Query: 124 SQEAKDFLGFNANANAKGSNASAVPAVSGGSSPQ----SPQVKNDGGMR---------RN 181
           S +A+ F        AKG N +   +   GSSP     SP   +D G           RN
Sbjct: 122 SPDAQVFYQL-----AKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRN 181

BLAST of Lag0010395 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-36
Identity = 76/179 (42.46%), Postives = 117/179 (65.36%), Query Frame = 0

Query: 4   KSSSSSALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDCCSGL 63
           KS+ +  ++  +  +L+GF +S++ QDR EC +Q++ L+TC+PYVGGDAKAPT DCC+G 
Sbjct: 3   KSTRTLFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGF 62

Query: 64  KLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPA-DVKDCIELLHLPPNSQ 123
             V++KS+KC+C+L++D+DDP LG+K+N TLA  LP+AC   A ++ DCI +LHLP NS 
Sbjct: 63  GQVIRKSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNST 122

Query: 124 EAKDFLGFNANANAKGSNASAVPAVSGGSSPQSPQVKNDGGMRRNQWFGVEIFIWFFIS 182
            AK+F       +   S +       G    ++  VK++ G +   W GVE+ I+  +S
Sbjct: 123 LAKEFENLGRIEDNYNSTSPTQIHKDGTGGGKAEPVKSN-GWKEKSWLGVELLIYLLVS 180

BLAST of Lag0010395 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-34
Identity = 82/196 (41.84%), Postives = 113/196 (57.65%), Query Frame = 0

Query: 4   KSSSSSALSCAMALILVGFV----SSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDC 63
           +S     ++ A+ALI+V  V     ++  +D+ EC  Q+VG+ATCLPYV G AK+PTPDC
Sbjct: 2   ESRKIKVMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDC 61

Query: 64  CSGLKLVLQKSKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPP 123
           CSGLK V+    KCLC++I++R+DP LGL+VN++LAL LP+ C + AD+  C  LLHL P
Sbjct: 62  CSGLKQVINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDP 121

Query: 124 NSQEAKDFL----GFNANANAKGSNASAVPAVSGGSSPQSPQVKNDGGMR---------- 181
           NS +A+ F     G N   +A     SA    S  S+P S    N G             
Sbjct: 122 NSPDAQVFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQS 181

BLAST of Lag0010395 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.0e-22
Identity = 65/157 (41.40%), Postives = 90/157 (57.32%), Query Frame = 0

Query: 10  ALSCAMALILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDCCSGLKLVLQK 69
           AL   +A++ +G V S + QD   C D +  L +CLP+V   AKAP   CCS LK+ + K
Sbjct: 15  ALCITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKIDK 74

Query: 70  --SKKCLCVLIKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPPNSQEAKDF 129
             ++KCLC L+KDRDDP LG KV+   A+ LP+AC  PA++  C +LLHL P+S  ++ F
Sbjct: 75  GQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDSPASQIF 134

Query: 130 LGFNANANAKGSNASAVPAVSGGSSPQSPQVKNDGGM 165
             F  ++    S      AVS  SS +    K  G M
Sbjct: 135 KQFTESS----SQTVGHKAVSTSSSIKGRDNKQFGLM 166

BLAST of Lag0010395 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 7.5e-10
Identity = 40/147 (27.21%), Postives = 68/147 (46.26%), Query Frame = 0

Query: 18  ILVGFVSSNIDQDRAECADQVVGLATCLPYVGGDAKAPTPDCCSGLKLVLQKSKKCLCVL 77
           + +  +SS +   ++ C + ++ ++ CL Y+ G++ +P   CC+ L  V+Q S  CLC +
Sbjct: 12  VFMAVMSSTMVSAQSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQV 71

Query: 78  IKDRDDPSLGLKVNLTLALGLPNACQSPADVKDCIELLHLPPNSQ---------EAKDFL 137
           + +     LG+ VN T ALGLP AC            +  PP S+            D  
Sbjct: 72  L-NGGGSQLGINVNQTQALGLPRACN-----------VQTPPVSRCNTGGGGGGSTSDSP 131

Query: 138 GFNANANAKGSNASAVPAVSGGSSPQS 156
             + N++  G+ +  VP   G   P S
Sbjct: 132 AESPNSSGPGNGSKTVPVGEGDGPPSS 146