Lag0010278 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0010278
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsubtilisin-like protease SBT4.3
Locationchr9: 46030222 .. 46032477 (+)
RNA-Seq ExpressionLag0010278
SyntenyLag0010278
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGATTAATCATCCCATCGTGATTCAGCTCATGGCGGCTCTTCTCGCCGCCGTTTGCTCGTCAACGTTTTGCGATTCTGGAAACATTCGCGAAAGAAAATTAAACATTGTGTACATGGGATCTATCGGAAACAGGGGAGCGGCGCAATTATCATCTACCCATTTGAATCTGCTCGAATCGGTCGTCGGAACTGCATCTGAAACAGAGCATTTGATGGTTAGAAGTTACAAAAGGAGCTTCAATGGCTTCGCTGCTCAGCTCACTGACAGCGAAGGAGAAAAGCTCGCCGCCATGAATGGGGTGATCTCTGTTTTTCCAAGCAAGGAACTCAAACCCCAAACCACAAGATCATGGGATTTTGTGGGTCTCCGTCAAAAAGCCAAGAGAAATGTCGTCAGTGAAAGCGACATCATAATTGGATCCTTCGACACCGGAATTTGGCCGGAATCAGAGAGCTTTAACGATGTGGGTCTCGGCCCGCCGCCGCAGCGGTGGAAAGGCACCTGCGCCGGCGGAGCAAATTTCACTTGCAACAAAAAGCTCATCGGCGCACGTTATTACTCAGCTTCATCATCAGCCAGAGACAGTACCGGGCATGGAACTCACACCGCCTCCACCGCCGCCGGAAGTCCAGTTGCAGGCGCCGAATTTTTCGGATTAGCCGCCGGAGTTGCAAGAGGAGCCGTCCCTTCTGCGAGAATCGCCGCTTACAAAGTCTGCGAATCCCGTTGCCATGAAGCCGATGTGTTGGCGGCGTTCGACGACGCCATCGCCGACGGAGTTGATCTGATTACAATTTCAGTCGGTGGGTCTGGTGGAAGCGCGTTTAAATTCGACGCCATCGCAATTGGGTCATTTCACGCCATGACTAAAGGAATCCTGACCGTCCAATCCGCCGGCAACAGTGGTCCGGCGGCGAGTACACTTACTAGCTCAGCTCCATGGCTCTTCACCATCGGAGCGACAACTACAGACAGAAGCATCGTTGACAGAGTCGTCCTCGGCAATGGCAAAACTGTAACTGGCTATACTGTAAACTCCTTCCCTCCAAATCCAAACGTTCTTCCATTGATTTACGCAACAAATGCTTCAAGAACTTGCCCTCGATCTCAATCTGAAAGATGCCAGCCAGGATGTCTCGATTCAGAGCTCGTCAAGGGGAAAATCGTTCAATGTATGACTTATAACGGAGCTTCTGAAGCTCTCAACGCCGGAGCTGCCGGAGCAATCGTTCTGAATGACAAATTAGTAAATGTTTCATTGGTCGCTCCATTTCCGATGACCGCCGTCAACGTCATGGATTACGGCGACGTCGCGAATTACGCCACCTCTGTTCAAAACCCAACTGCCAGAATTCTCAAAAGTCAAGCCATTAAAGACGTCTCTGCTCCGCGTGCAGCTACATTCTCCAGCCGCGGTCCGAACGCGGTGATGCCGGAGATCATGAAGCCCGACGTCTCCGCTCCCGGCGTCGAAATCCTCGCCGCCTTTCCGCCTGTGCTATTGCCTGCCGATCTTCCCGGATTTAAACAATTTGTCAACTACTCCATAATGTCTGGAACTTCCATGTCTTGCCCTCACGCCGCCGGTGTCGCGGCGTATGTGAAGAGCTTCCATCGAGATTGGTCGCCGTCGGCGATCAAGTCCGCTATCATGACGACGGCGAAACCGATCACGAGCAACGACGGCACGATCATCGGAGAGTTTCTTCATGGGTCAGGCCAAATCAATCCAAAGGAGGCCCTCAAACCCGGCCTTGTTTATGAAACTTCTAAAGAAGATTACATAAACTTGCTGTGTGGAAATGGCTATGATTCAAAGGATATTCAGCTCATGTCCGGAGATTTTAACAGTAGCTGTTCATCAAATGTGACGAATTTTCCCGCTAGAGATTTGAATTACCCTGCAATGGCGGCTCAAGTTTTACCGACGCAGGCATTTGTGGTAAAATTTGTGAGAATAGTTACCAATGTTGGGGATGGAAGCTCGAGTTACAAGGCAGAATTTTTACCGTTTAGTAAAGTTTACTTGACCGAGTCAACTGAAAAGCTCAATGCGAGCGTTGAGCCGCAAGAGCTGTCGTTTGGAGCAGTAAATGAGAAGAAGAGTTTTACTGTGACGGTGGCCGGAGGGTTAATTCCTGATGGAACGGTGTTTTCTACCGCATTGGTTTGGAGTGATGGGATCCATAAGGTGAGAAGTCCCATTGTTGTGGAAGTCAAATCACAATCAACTATTCCTACAAATTAA

mRNA sequence

ATGGCGAAGATTAATCATCCCATCGTGATTCAGCTCATGGCGGCTCTTCTCGCCGCCGTTTGCTCGTCAACGTTTTGCGATTCTGGAAACATTCGCGAAAGAAAATTAAACATTGTGTACATGGGATCTATCGGAAACAGGGGAGCGGCGCAATTATCATCTACCCATTTGAATCTGCTCGAATCGGTCGTCGGAACTGCATCTGAAACAGAGCATTTGATGGTTAGAAGTTACAAAAGGAGCTTCAATGGCTTCGCTGCTCAGCTCACTGACAGCGAAGGAGAAAAGCTCGCCGCCATGAATGGGGTGATCTCTGTTTTTCCAAGCAAGGAACTCAAACCCCAAACCACAAGATCATGGGATTTTGTGGGTCTCCGTCAAAAAGCCAAGAGAAATGTCGTCAGTGAAAGCGACATCATAATTGGATCCTTCGACACCGGAATTTGGCCGGAATCAGAGAGCTTTAACGATGTGGGTCTCGGCCCGCCGCCGCAGCGGTGGAAAGGCACCTGCGCCGGCGGAGCAAATTTCACTTGCAACAAAAAGCTCATCGGCGCACGTTATTACTCAGCTTCATCATCAGCCAGAGACAGTACCGGGCATGGAACTCACACCGCCTCCACCGCCGCCGGAAGTCCAGTTGCAGGCGCCGAATTTTTCGGATTAGCCGCCGGAGTTGCAAGAGGAGCCGTCCCTTCTGCGAGAATCGCCGCTTACAAAGTCTGCGAATCCCGTTGCCATGAAGCCGATGTGTTGGCGGCGTTCGACGACGCCATCGCCGACGGAGTTGATCTGATTACAATTTCAGTCGGTGGGTCTGGTGGAAGCGCGTTTAAATTCGACGCCATCGCAATTGGGTCATTTCACGCCATGACTAAAGGAATCCTGACCGTCCAATCCGCCGGCAACAGTGGTCCGGCGGCGAGTACACTTACTAGCTCAGCTCCATGGCTCTTCACCATCGGAGCGACAACTACAGACAGAAGCATCGTTGACAGAGTCGTCCTCGGCAATGGCAAAACTGTAACTGGCTATACTGTAAACTCCTTCCCTCCAAATCCAAACGTTCTTCCATTGATTTACGCAACAAATGCTTCAAGAACTTGCCCTCGATCTCAATCTGAAAGATGCCAGCCAGGATGTCTCGATTCAGAGCTCGTCAAGGGGAAAATCGTTCAATGTATGACTTATAACGGAGCTTCTGAAGCTCTCAACGCCGGAGCTGCCGGAGCAATCGTTCTGAATGACAAATTAGTAAATGTTTCATTGGTCGCTCCATTTCCGATGACCGCCGTCAACGTCATGGATTACGGCGACGTCGCGAATTACGCCACCTCTGTTCAAAACCCAACTGCCAGAATTCTCAAAAGTCAAGCCATTAAAGACGTCTCTGCTCCGCGTGCAGCTACATTCTCCAGCCGCGGTCCGAACGCGGTGATGCCGGAGATCATGAAGCCCGACGTCTCCGCTCCCGGCGTCGAAATCCTCGCCGCCTTTCCGCCTGTGCTATTGCCTGCCGATCTTCCCGGATTTAAACAATTTGTCAACTACTCCATAATGTCTGGAACTTCCATGTCTTGCCCTCACGCCGCCGGTGTCGCGGCGTATGTGAAGAGCTTCCATCGAGATTGGTCGCCGTCGGCGATCAAGTCCGCTATCATGACGACGGCGAAACCGATCACGAGCAACGACGGCACGATCATCGGAGAGTTTCTTCATGGGTCAGGCCAAATCAATCCAAAGGAGGCCCTCAAACCCGGCCTTGTTTATGAAACTTCTAAAGAAGATTACATAAACTTGCTGTGTGGAAATGGCTATGATTCAAAGGATATTCAGCTCATGTCCGGAGATTTTAACAGTAGCTGTTCATCAAATGTGACGAATTTTCCCGCTAGAGATTTGAATTACCCTGCAATGGCGGCTCAAGTTTTACCGACGCAGGCATTTGTGGTAAAATTTGTGAGAATAGTTACCAATGTTGGGGATGGAAGCTCGAGTTACAAGGCAGAATTTTTACCGTTTAGTAAAGTTTACTTGACCGAGTCAACTGAAAAGCTCAATGCGAGCGTTGAGCCGCAAGAGCTGTCGTTTGGAGCAGTAAATGAGAAGAAGAGTTTTACTGTGACGGTGGCCGGAGGGTTAATTCCTGATGGAACGGTGTTTTCTACCGCATTGGTTTGGAGTGATGGGATCCATAAGGTGAGAAGTCCCATTGTTGTGGAAGTCAAATCACAATCAACTATTCCTACAAATTAA

Coding sequence (CDS)

ATGGCGAAGATTAATCATCCCATCGTGATTCAGCTCATGGCGGCTCTTCTCGCCGCCGTTTGCTCGTCAACGTTTTGCGATTCTGGAAACATTCGCGAAAGAAAATTAAACATTGTGTACATGGGATCTATCGGAAACAGGGGAGCGGCGCAATTATCATCTACCCATTTGAATCTGCTCGAATCGGTCGTCGGAACTGCATCTGAAACAGAGCATTTGATGGTTAGAAGTTACAAAAGGAGCTTCAATGGCTTCGCTGCTCAGCTCACTGACAGCGAAGGAGAAAAGCTCGCCGCCATGAATGGGGTGATCTCTGTTTTTCCAAGCAAGGAACTCAAACCCCAAACCACAAGATCATGGGATTTTGTGGGTCTCCGTCAAAAAGCCAAGAGAAATGTCGTCAGTGAAAGCGACATCATAATTGGATCCTTCGACACCGGAATTTGGCCGGAATCAGAGAGCTTTAACGATGTGGGTCTCGGCCCGCCGCCGCAGCGGTGGAAAGGCACCTGCGCCGGCGGAGCAAATTTCACTTGCAACAAAAAGCTCATCGGCGCACGTTATTACTCAGCTTCATCATCAGCCAGAGACAGTACCGGGCATGGAACTCACACCGCCTCCACCGCCGCCGGAAGTCCAGTTGCAGGCGCCGAATTTTTCGGATTAGCCGCCGGAGTTGCAAGAGGAGCCGTCCCTTCTGCGAGAATCGCCGCTTACAAAGTCTGCGAATCCCGTTGCCATGAAGCCGATGTGTTGGCGGCGTTCGACGACGCCATCGCCGACGGAGTTGATCTGATTACAATTTCAGTCGGTGGGTCTGGTGGAAGCGCGTTTAAATTCGACGCCATCGCAATTGGGTCATTTCACGCCATGACTAAAGGAATCCTGACCGTCCAATCCGCCGGCAACAGTGGTCCGGCGGCGAGTACACTTACTAGCTCAGCTCCATGGCTCTTCACCATCGGAGCGACAACTACAGACAGAAGCATCGTTGACAGAGTCGTCCTCGGCAATGGCAAAACTGTAACTGGCTATACTGTAAACTCCTTCCCTCCAAATCCAAACGTTCTTCCATTGATTTACGCAACAAATGCTTCAAGAACTTGCCCTCGATCTCAATCTGAAAGATGCCAGCCAGGATGTCTCGATTCAGAGCTCGTCAAGGGGAAAATCGTTCAATGTATGACTTATAACGGAGCTTCTGAAGCTCTCAACGCCGGAGCTGCCGGAGCAATCGTTCTGAATGACAAATTAGTAAATGTTTCATTGGTCGCTCCATTTCCGATGACCGCCGTCAACGTCATGGATTACGGCGACGTCGCGAATTACGCCACCTCTGTTCAAAACCCAACTGCCAGAATTCTCAAAAGTCAAGCCATTAAAGACGTCTCTGCTCCGCGTGCAGCTACATTCTCCAGCCGCGGTCCGAACGCGGTGATGCCGGAGATCATGAAGCCCGACGTCTCCGCTCCCGGCGTCGAAATCCTCGCCGCCTTTCCGCCTGTGCTATTGCCTGCCGATCTTCCCGGATTTAAACAATTTGTCAACTACTCCATAATGTCTGGAACTTCCATGTCTTGCCCTCACGCCGCCGGTGTCGCGGCGTATGTGAAGAGCTTCCATCGAGATTGGTCGCCGTCGGCGATCAAGTCCGCTATCATGACGACGGCGAAACCGATCACGAGCAACGACGGCACGATCATCGGAGAGTTTCTTCATGGGTCAGGCCAAATCAATCCAAAGGAGGCCCTCAAACCCGGCCTTGTTTATGAAACTTCTAAAGAAGATTACATAAACTTGCTGTGTGGAAATGGCTATGATTCAAAGGATATTCAGCTCATGTCCGGAGATTTTAACAGTAGCTGTTCATCAAATGTGACGAATTTTCCCGCTAGAGATTTGAATTACCCTGCAATGGCGGCTCAAGTTTTACCGACGCAGGCATTTGTGGTAAAATTTGTGAGAATAGTTACCAATGTTGGGGATGGAAGCTCGAGTTACAAGGCAGAATTTTTACCGTTTAGTAAAGTTTACTTGACCGAGTCAACTGAAAAGCTCAATGCGAGCGTTGAGCCGCAAGAGCTGTCGTTTGGAGCAGTAAATGAGAAGAAGAGTTTTACTGTGACGGTGGCCGGAGGGTTAATTCCTGATGGAACGGTGTTTTCTACCGCATTGGTTTGGAGTGATGGGATCCATAAGGTGAGAAGTCCCATTGTTGTGGAAGTCAAATCACAATCAACTATTCCTACAAATTAA

Protein sequence

MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNVGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTALVWSDGIHKVRSPIVVEVKSQSTIPTN
Homology
BLAST of Lag0010278 vs. NCBI nr
Match: XP_022958362.1 (subtilisin-like protease SBT4.3 [Cucurbita moschata])

HSP 1 Score: 900.6 bits (2326), Expect = 9.2e-258
Identity = 479/751 (63.78%), Postives = 574/751 (76.43%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA IN PI+   +AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAAINFPILFTFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT  E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS SSSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P TA+N   Y  VANYA S  NP  RILKS AIKD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           PEIMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE  ++DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + FVVKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F  +NEKKSF VTVAGG IP  T 
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS AL+WSDGIHKVRSPIVV VK    IPT+
Sbjct: 721 FSLALIWSDGIHKVRSPIVVNVK-LPPIPTS 738

BLAST of Lag0010278 vs. NCBI nr
Match: KAG6605933.1 (Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 899.4 bits (2323), Expect = 2.1e-257
Identity = 476/751 (63.38%), Postives = 571/751 (76.03%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI    +AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAANNFPIFFSFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT  E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS SSSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P  A+N   Y  V NYA S  NP  RILKS AIKD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVTNYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           PEIMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE  ++DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + FVVKF RIVTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRIVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F  +NEKKSF VTVAGG IP  T 
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPTELTFKDLNEKKSFVVTVAGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS+AL+WSDGIHKVRSPIVV VK   T  +N
Sbjct: 721 FSSALIWSDGIHKVRSPIVVNVKLPPTPTSN 739

BLAST of Lag0010278 vs. NCBI nr
Match: XP_023533940.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.9 bits (2288), Expect = 2.4e-253
Identity = 469/751 (62.45%), Postives = 572/751 (76.17%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI    +AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAANNFPISFSFIAALLAAIFT-----SANGSERKDHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
           +SV+GT+S TE   +RSY RSFNGF A+LT  E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61  QSVIGTSSVTEDSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGMGPPPQKWRGECAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS SSSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P  A+N+  Y  VANYA S  NP  RIL+S A+KD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNLTAYEQVANYAISEPNPHVRILQSVAVKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           PEIMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQI+PK+A++PGLVYE  ++DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFLSGEFLHGSGQIDPKQAIEPGLVYEIFEDDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + F VKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFAVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF P S VY+ +S+EKLN SVEP EL+F  +NEKKSF VTVAGG IP  T 
Sbjct: 661 GIANSTYKSEFSPLSVVYVLKSSEKLNVSVEPPELTFRDLNEKKSFVVTVAGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS+AL+WSDGIHKVRSP+V+ VK    IPT+
Sbjct: 721 FSSALIWSDGIHKVRSPMVLNVK-LPPIPTS 738

BLAST of Lag0010278 vs. NCBI nr
Match: XP_022995032.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])

HSP 1 Score: 882.5 bits (2279), Expect = 2.6e-252
Identity = 469/751 (62.45%), Postives = 567/751 (75.50%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI+  L+AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAPNNFPILFSLIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT +E E+LA M+GV+SVF SKELK QTTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS +SSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-TSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM +GILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSI+D  VLGNGK V+GY++N F PN NV  LI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNV-TLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P  A+N   Y  VANYA S  NP  RILKS AIKD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           P+IMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PDIMKPDIAAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQINPK+A++PGLVYE  + DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + FV+KF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF  FS VY+ +S+EK N SVEP EL+F  +NEKKSF VTV GG IP  T 
Sbjct: 661 GIANSTYKSEFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS+ALVWSDGIHKVRSPIVV VK    IPT+
Sbjct: 721 FSSALVWSDGIHKVRSPIVVNVK-LPPIPTS 738

BLAST of Lag0010278 vs. NCBI nr
Match: XP_022995031.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])

HSP 1 Score: 876.3 bits (2263), Expect = 1.9e-250
Identity = 464/743 (62.45%), Postives = 556/743 (74.83%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI+   +AALLAA+ S     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAPDNFPILFSFIAALLAAILS-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT +E E+LAAM+GV+SVF SK  K QTTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           D++G   K  RN+  E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTCAGG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGAR+Y+ S SARD+ GHG+HTASTAAG       F+GLA G ARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYK 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
           VC   C E ++LAAFDDAIADGVDLITIS+GG GG  F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 VCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   T+ S  PW+FT+ ATTTDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           YATNASR C    +E C  GCLD  LVKGKIVQC  ++GAS A NAGAAGAIVLND   N
Sbjct: 361 YATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS V PFP  A+ + DY  VANYA S  NP   I +S A KD  AP  A FSSRGPN  M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
            EI+KPD++APGVEILA+F P+  P+   G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSP+AIKSAIMTTAK I   DG +I EFL+GSG I+P  A++PGLVYE  ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNV 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LC  GYDSK ++  +G+ +S CS   T F ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMEAFAGN-DSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+Y+++ L FS V + +S EKLN SV+PQEL F  +NEKKSF VTV GG I    V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
           FS+AL+WSD  H+VRSPIVV +K
Sbjct: 721 FSSALIWSDLDHEVRSPIVVMIK 734

BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 2.0e-202
Identity = 385/742 (51.89%), Postives = 497/742 (66.98%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MAK++ P+ +  +A +     S+    +   +   + IVYMG++     +   S HL++L
Sbjct: 1   MAKLSTPLYLICLAFIFTRDVSA----NDYRQASSVYIVYMGTLPEIKYSP-PSHHLSIL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
           + +VGT +   HL+VRSYKRSFNGFAA L+ +E +KL  M  V+SVFPSK  +  TTRSW
Sbjct: 61  QKLVGTIA-ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DFVG  +KA+R  V ESD+I+G  D+GIWPESESF+D G GPPP++WKG+C GG  F CN
Sbjct: 121 DFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACN 180

Query: 181 KKLIGARYYSA-SSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAY 240
            KLIGAR+Y+  + SARD  GHGTHTASTAAG+ V  A F+GLA G ARG VPSARIAAY
Sbjct: 181 NKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAY 240

Query: 241 KVCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQ 300
           KVC +RC++ D+LAAFDDAIADGVD+I+IS+     S     ++AIGSFHAM +GI+T  
Sbjct: 241 KVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAG 300

Query: 301 SAGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPL 360
           SAGN+GP   ++ + +PW+ T+ A+ TDR  +DRVVLGNGK +TG +VN+F  N    P+
Sbjct: 301 SAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPI 360

Query: 361 IYATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLV 420
           +Y  N SR C ++Q+  C  GC+DSELVKGKIV C  + G  EA  AGA G IV N  L 
Sbjct: 361 VYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLP 420

Query: 421 NVSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAV 480
           + + V PFP +++   DY  + +Y  S + P A IL+++ I D  AP   +FSSRGP+ V
Sbjct: 421 DSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFV 480

Query: 481 MPEIMKPDVSAPGVEILAAFPPVLLPADL--PGFKQFVNYSIMSGTSMSCPHAAGVAAYV 540
           +  ++KPDVSAPG+EILAAF PV  P+    P  K+ V YS+MSGTSM+CPH AGVAAYV
Sbjct: 481 IQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYV 540

Query: 541 KSFHRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDY 600
           KSFH DWSPSAIKSAIMTTA P+         EF +GSGQINP +A  PGLVYE   EDY
Sbjct: 541 KSFHPDWSPSAIKSAIMTTATPMNLKKNP-EQEFAYGSGQINPTKASDPGLVYEVETEDY 600

Query: 601 INLLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIV 660
           + +LC  G+DS  +   SG  N +CS        +DLNYP M   V     F V F R V
Sbjct: 601 LKMLCAEGFDSTTLTTTSGQ-NVTCSERT---EVKDLNYPTMTTFVSSLDPFNVTFKRTV 660

Query: 661 TNVGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPD 720
           TNVG  +S+YKA  +P           +L  S+EP+ L FG + EKKSF VT++G  + D
Sbjct: 661 TNVGFPNSTYKASVVPLQ--------PELQISIEPEILRFGFLEEKKSFVVTISGKELKD 720

Query: 721 GTVFSTALVWSDGIHKVRSPIV 740
           G+  S+++VWSDG H VRSPIV
Sbjct: 721 GSFVSSSVVWSDGSHSVRSPIV 723

BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 2.9e-190
Identity = 365/744 (49.06%), Postives = 509/744 (68.41%), Query Frame = 0

Query: 10  IQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASE 69
           I L ++LL    SS   D  +  ++++ IVY+GS+ +R      S H+++L+ + G  S 
Sbjct: 8   IFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITG-ESL 67

Query: 70  TEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ-- 129
            E+ +VRSYK+SFNGFAA+LT+SE ++LA M  V+SVFPS++LK QTT SW+F+GL++  
Sbjct: 68  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 127

Query: 130 KAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGAR 189
           K KR    ESD IIG  D+GI+PES+SF+D G GPPP++WKGTCAGG NFTCN K+IGAR
Sbjct: 128 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 187

Query: 190 YYSASS----SARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE 249
            Y+A S    +ARD +GHGTHTAS AAG+ VA + F+GL  G ARG VP+ARIA YKVC+
Sbjct: 188 DYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCD 247

Query: 250 SR-CHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAG 309
           +  C    +++AFDDAIADGVD+I+IS+       F+ D IAIG+FHAM  G+LTV +AG
Sbjct: 248 NEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAG 307

Query: 310 NSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYA 369
           N+GP  ST+TS+APW+F++ A+ T+R+ + +VVLG+GK + G +VN++  N    PL+Y 
Sbjct: 308 NNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYG 367

Query: 370 TNAS-RTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 429
            +A+  TC   ++  C+P CLD +LVKGKIV C +  G  EA   GA G+IV N +  + 
Sbjct: 368 KSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE-PDR 427

Query: 430 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 489
           + +  FP++ ++  DY  + +Y  S +NP A +LKS+ I +  AP  A+FSSRGP++++ 
Sbjct: 428 AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVS 487

Query: 490 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 549
           +I+KPD++APGVEILAA+ P   P +     + V YS++SGTSM+CPH AGVAAYVK+FH
Sbjct: 488 DILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFH 547

Query: 550 RDWSPSAIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 609
             WSPS I+SAIMTTA P+  S  G +  EF +GSG ++P +A+ PGLVYE +K D+IN 
Sbjct: 548 PQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINF 607

Query: 610 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 669
           LCG  Y S  ++++SGD NS+C+  ++    R+LNYP M+A+V  T+ F + F R VTNV
Sbjct: 608 LCGLNYTSDHLRIISGD-NSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 667

Query: 670 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 729
           G   S+Y A+ + F          KL+  V P+ LS  ++NEK+SF VTV+   I     
Sbjct: 668 GMQKSTYNAKVVKF-------PGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 727

Query: 730 FSTALVWSDGIHKVRSPIVVEVKS 745
            S  L+WSDG H VRSPI+V   S
Sbjct: 728 VSANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 2.5e-189
Identity = 367/741 (49.53%), Postives = 494/741 (66.67%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ + A +  S      + +++++ IVYMG++ +R      S H ++L+ V G +S  +
Sbjct: 9   LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
            L VR+YKRSFNGFAA+LT+SE E LA+M+ V+SVFPSK L  QTT SW+F+GL++  + 
Sbjct: 69  RL-VRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 128

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN + ESD IIG  D+GI+PES+SF+  G GPPP++WKG C GG NFTCN KLIGARYY
Sbjct: 129 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY 188

Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCES 251
           +        SARD+TGHG+HTAS AAG+ V    F+GL  G  RG VP+ARIA YKVC+ 
Sbjct: 189 TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDP 248

Query: 252 ---RCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
              RC    +LAAFDDAIAD VD+IT+S+G      F+ D +AIG+FHAM KGILTV  A
Sbjct: 249 GVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGA 308

Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
           GN+GP   T+ S APWLFT+ A+  +R+ + +VVLGNGKT+ G +VNSF  N    PL+Y
Sbjct: 309 GNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY 368

Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
             +AS  C  S +  C PGCLDS+ VKGKIV C T     EA   GA  +IV N    + 
Sbjct: 369 GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDA 428

Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
           + V  FP++ ++  DY  V +Y  S +NP A +LKS+ I +  AP  A++SSRGPN ++ 
Sbjct: 429 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 488

Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
           +I+KPD++APG EILAA+ P + P++     + V Y+++SGTSMSCPH AGVAAY+K+FH
Sbjct: 489 DILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 548

Query: 552 RDWSPSAIKSAIMTTAKPI--TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
             WSPS I+SAIMTTA P+  +++    + EF +G+G ++P  A+ PGLVYE +K D+I 
Sbjct: 549 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 608

Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
            LCG  Y  K ++L+SGD +SSC+   T    R+LNYP+M+AQV  T+ F V F R VTN
Sbjct: 609 FLCGFNYTGKKLRLISGD-SSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTN 668

Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
           VG  +++YKA+ +            KL   V P  LS  ++ EKKSFTVTV+G       
Sbjct: 669 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 728

Query: 732 VFSTALVWSDGIHKVRSPIVV 741
           + S  L+WSDG+H VRSPIVV
Sbjct: 729 LVSAQLIWSDGVHFVRSPIVV 734

BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match: F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 2.8e-185
Identity = 369/741 (49.80%), Postives = 492/741 (66.40%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ + A +  S      + ++++  IVYMG++  R      S H ++L+ V G +S  E
Sbjct: 10  LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESS-IE 69

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
             +VR+YKRSFNGFAA+LT SE E LA+M+ V+SVFP+K+LK QTT SW+F+GL++  + 
Sbjct: 70  DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN + ESD IIG  D+GI+PES+SF+  G GPPP++WKG C GG NFT N KLIGARYY
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189

Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE- 251
           +        SARD  GHG+HTASTAAG+ V    F+GL  G ARG VP+ARIA YKVC+ 
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249

Query: 252 --SRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
               C    +LAAFDDAIAD VD+ITIS+GG   S F+ D IAIG+FHAM KGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309

Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
           GNSGP  ST+ S APW+FT+ A+ T+R+ V +VVLGNGKTV G +VNSF  N    PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369

Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
             +AS +C  + +  C PGCLDS+ VKGKIV C +     EA   GA  +IV + +  +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR-TDV 429

Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
           + +  FP++ +   DY  V +Y  S +NP A +LKS+ I +  AP  A++ SRGPN ++P
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489

Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
           +I+KPD++APG EI+AA+ P   P+      + V YS+ +GTSMSCPH AGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549

Query: 552 RDWSPSAIKSAIMTTAKPITSNDGTI--IGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
             WSPS I+SAIMTTA P+ ++      + EF +G+G ++P  A+ PGLVYE +K D+I 
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609

Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
            LCG  Y +K+++L+SGD +SSC+   T    R+LNYP+M AQV   + F V F R VTN
Sbjct: 610 FLCGLNYTAKNLRLISGD-SSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTN 669

Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
           VG  +++YKA+ +            KL   V P  LS  ++ EKKSFTVT +G       
Sbjct: 670 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAEN 729

Query: 732 VFSTALVWSDGIHKVRSPIVV 741
           + S  L+WSDG+H VRSPIVV
Sbjct: 730 LVSAQLIWSDGVHFVRSPIVV 734

BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 5.6e-181
Identity = 364/735 (49.52%), Postives = 482/735 (65.58%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ LL    SS    S    ++++ IVYMGS+ +R     +S H+N+L+ V G +S  E
Sbjct: 10  LLSCLLVLFLSSV---SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESS-IE 69

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
             +VRSYKRSFNGFAA+LT+SE E++A M GV+SVFP+K+L+ QTT SWDF+GL++  K 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKT 129

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN   ESD IIG  D+GI PES+SF+D G GPPPQ+WKG C+GG NFTCN KLIGAR Y
Sbjct: 130 KRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 192 SASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHE 251
           + S   RD  GHGTHTASTAAG+ V  A FFG+  G  RG VP++R+AAYKVC  + C  
Sbjct: 190 T-SEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSS 249

Query: 252 ADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAA 311
             +L+AFDDAIADGVDLITIS+G    S F+ D IAIG+FHAM KG+LTV SAGNSGP  
Sbjct: 250 EALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKP 309

Query: 312 STLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATN-ASR 371
            +++  APW+ T+ A+TT+R  V +VVLGNGKT+ G +VN++       PL+Y  + AS 
Sbjct: 310 ISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASS 369

Query: 372 TCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPF 431
            C    +  C+  C+D   VKGKI+ C    G     + GA G I    K  +V+ + P 
Sbjct: 370 ACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPK-PDVAFIHPL 429

Query: 432 PMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPD 491
           P   +   D+  + +Y  S  +P A +LK++AI + ++P  A+FSSRGPN +  +I+KPD
Sbjct: 430 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 489

Query: 492 VSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPS 551
           ++APGVEILAA+ P   P+      + V YS++SGTSMSCPH AGVAAYVK+F+  WSPS
Sbjct: 490 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 549

Query: 552 AIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGY 611
            I+SAIMTTA P+  +  G    EF +GSG ++P  A  PGLVYE  K D+I  LCG  Y
Sbjct: 550 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 609

Query: 612 DSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPT-QAFVVKFVRIVTNVGDGSS 671
            S+ ++++SG+   +CS      P R+LNYP+M+A++  +   F V F R +TNVG  +S
Sbjct: 610 TSQVLKVISGE-TVTCSEAKKILP-RNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNS 669

Query: 672 SYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTAL 731
           +Y       SKV     + KL+  + P  LSF  VNEK+SFTVTV G  +      S  L
Sbjct: 670 TYT------SKVVAGHGS-KLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 727

Query: 732 VWSDGIHKVRSPIVV 741
           +WSDG H VRSPIVV
Sbjct: 730 IWSDGTHNVRSPIVV 727

BLAST of Lag0010278 vs. ExPASy TrEMBL
Match: A0A6J1H1M3 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE=3 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 4.5e-258
Identity = 479/751 (63.78%), Postives = 574/751 (76.43%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA IN PI+   +AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAAINFPILFTFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT  E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS SSSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P TA+N   Y  VANYA S  NP  RILKS AIKD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           PEIMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE  ++DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + FVVKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F  +NEKKSF VTVAGG IP  T 
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS AL+WSDGIHKVRSPIVV VK    IPT+
Sbjct: 721 FSLALIWSDGIHKVRSPIVVNVK-LPPIPTS 738

BLAST of Lag0010278 vs. ExPASy TrEMBL
Match: A0A6J1JXM0 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 1.3e-252
Identity = 469/751 (62.45%), Postives = 567/751 (75.50%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI+  L+AALLAA+ +     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAPNNFPILFSLIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT +E E+LA M+GV+SVF SKELK QTTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           DF+G  Q+ KRN+  E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGARYYS +SSARD  GHGTHTASTA G  V    F+G+A GVARGAVPS+R+A Y 
Sbjct: 181 HKVIGARYYS-TSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C + ++LAAFDDAIADGVD+ITIS+GG   S  + D IAIG++HAM +GILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   ++ S APWLF++ ATTTDRSI+D  VLGNGK V+GY++N F PN NV  LI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNV-TLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           Y TNAS++C     E C   CLD +LVKGKIVQC ++ G  +A++AGAAGAIVLNDK  N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS + P P  A+N   Y  VANYA S  NP  RILKS AIKD  AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
           P+IMKPD++APGVEILA+  P    +D PG  + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PDIMKPDIAAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQINPK+A++PGLVYE  + DY+  
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKF 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LCGNG+DSK ++ +SG+  S CS   T F  +DLNYPAM  +V P + FV+KF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+YK+EF  FS VY+ +S+EK N SVEP EL+F  +NEKKSF VTV GG IP  T 
Sbjct: 661 GIANSTYKSEFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTG 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
           FS+ALVWSDGIHKVRSPIVV VK    IPT+
Sbjct: 721 FSSALVWSDGIHKVRSPIVVNVK-LPPIPTS 738

BLAST of Lag0010278 vs. ExPASy TrEMBL
Match: A0A6J1K0V8 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 9.0e-251
Identity = 464/743 (62.45%), Postives = 556/743 (74.83%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI+   +AALLAA+ S     S N  ERK +IVYMG+I NR  A+ S+ HLNLL
Sbjct: 1   MAPDNFPILFSFIAALLAAILS-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S TE   +RSY RSFNGF A+LT +E E+LAAM+GV+SVF SK  K QTTRSW
Sbjct: 61  RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           D++G   K  RN+  E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTCAGG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGAR+Y+ S SARD+ GHG+HTASTAAG       F+GLA G ARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYK 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
           VC   C E ++LAAFDDAIADGVDLITIS+GG GG  F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 VCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   T+ S  PW+FT+ ATTTDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           YATNASR C    +E C  GCLD  LVKGKIVQC  ++GAS A NAGAAGAIVLND   N
Sbjct: 361 YATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS V PFP  A+ + DY  VANYA S  NP   I +S A KD  AP  A FSSRGPN  M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
            EI+KPD++APGVEILA+F P+  P+   G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSP+AIKSAIMTTAK I   DG +I EFL+GSG I+P  A++PGLVYE  ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNV 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LC  GYDSK ++  +G+ +S CS   T F ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMEAFAGN-DSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+Y+++ L FS V + +S EKLN SV+PQEL F  +NEKKSF VTV GG I    V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
           FS+AL+WSD  H+VRSPIVV +K
Sbjct: 721 FSSALIWSDLDHEVRSPIVVMIK 734

BLAST of Lag0010278 vs. ExPASy TrEMBL
Match: A0A6J1H1W0 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 1.0e-246
Identity = 454/743 (61.10%), Postives = 552/743 (74.29%), Query Frame = 0

Query: 1   MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
           MA  N PI+   +AALLAA+ +     S N  ERK +IVYMG++ NR  A+ S+ HLNLL
Sbjct: 1   MAPENFPILFSFIAALLAAIFT-----SANGSERKAHIVYMGAVQNRAMAE-STHHLNLL 60

Query: 61  ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
            SV+GT+S  E   +RSY RSFNGF A+LT  E E+LAAM+GV+SVF SK  K QTTRSW
Sbjct: 61  RSVIGTSSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSW 120

Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
           D++G   K  RN+  E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTC GG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCTGGGNFTCN 180

Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
            K+IGAR+Y+ S SARD+ GHG+HTASTAAG       F+GLA GVARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAIYK 240

Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
            C   C E ++LAAFDDAIADGVDLITIS+ G+GG  F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 ACNPDCLEENILAAFDDAIADGVDLITISIVGAGGFNFEHDSMAIGSYHSMAKGILTVQS 300

Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
           AGN GP   T+ S  PW+FT+ AT TDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPEDGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360

Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
           YATNASR C    +E C+ GCLD  LVKGKIVQC  ++GAS A NAGAAGAIVLND   N
Sbjct: 361 YATNASRNCSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420

Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
           VS V PFP  A+ + DY  VANYA S  NP   I +S A KD  AP  A FSSRGPN  M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPHAPMIADFSSRGPNMWM 480

Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
            EI+KPD++APGVEILA+F P+  P+   G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540

Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
           H +WSP+AIKSAIMTTAK I   DG +I EFL+GSG ++P  A++PGLVYE  ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLVDPDRAIEPGLVYEIFEKDHLNM 600

Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
           LC  GYDSK ++  +G+ +S C    T + ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMKAFAGN-DSVCPKTWTKYLARDLNYPAMVAHVLPMKPFVVKFQRTVTNV 660

Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
           G  +S+Y+++ L FS V + +S EKL+ SV+PQ+L FG +NEKKSF VTV GG IP   V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSLEKLSVSVKPQKLWFGNLNEKKSFVVTVVGGTIPTKIV 720

Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
            S+ L+WSD  H+VRSPIVV VK
Sbjct: 721 LSSVLIWSDLDHEVRSPIVVMVK 734

BLAST of Lag0010278 vs. ExPASy TrEMBL
Match: A0A4P1R6C9 (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_29596 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 3.8e-209
Identity = 392/723 (54.22%), Postives = 506/723 (69.99%), Query Frame = 0

Query: 28  SGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETEHLMVRSYKRSFNGFAA 87
           +G   +RK+ I+YMGS+     +   S H+N+L+ V+G   ++ HL V SYKRSFNGFAA
Sbjct: 23  NGANEDRKVYIIYMGSLPKENYSP-QSEHINILQQVLGDDFDSNHL-VTSYKRSFNGFAA 82

Query: 88  QLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQKAKRNVVSESDIIIGSFDTG 147
           +LTD E + +  M GV+S+F SK L+  TTRSWDF+GL++K K + + ESD+IIG FDTG
Sbjct: 83  KLTDQEVKNIGRMRGVVSIFESKTLQLHTTRSWDFIGLQEKTKTSPI-ESDMIIGVFDTG 142

Query: 148 IWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYYSASSSARDSTGHGTHTAS 207
           IWPESESF+D G GPPP++WKGTCAGG NFTCN K+IGARYYS + SARDS GHGTHTAS
Sbjct: 143 IWPESESFDDEGFGPPPKKWKGTCAGGKNFTCNNKIIGARYYSENVSARDSVGHGTHTAS 202

Query: 208 TAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHEADVLAAFDDAIADGVDLI 267
           TAAG+ V G  F+G+A G ARG VPSARIA YKVC +S+C +A +LAAFDDAIADGVDL+
Sbjct: 203 TAAGNKVKGTSFYGIAEGTARGGVPSARIAVYKVCDDSQCSDAAILAAFDDAIADGVDLV 262

Query: 268 TISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAASTLTSSAPWLFTIGATTT 327
           TIS+GGS   +F  D  AIGSFHAM KGILTV S GN GP  ++  S APWLF++ A+ T
Sbjct: 263 TISIGGSTPRSFTEDPNAIGSFHAMEKGILTVNSGGNGGPIKTSTASLAPWLFSVAASNT 322

Query: 328 DRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATNASR-TCPRSQSERCQPGCLDSE 387
           DR I+D+V LGNG T+TG +VN+F PN   +PL+ A   S+  CP   S  C P CLD E
Sbjct: 323 DRRIIDKVSLGNGVTLTGQSVNTFTPNGTKIPLVLAEKVSKPNCPIPSS--CFPNCLDPE 382

Query: 388 LVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPFPMTAVNVMDYGDVANYAT 447
            V+GKIV C +  G    + AGA G+I+ +D+  NV+ V PFP T ++   Y     Y T
Sbjct: 383 KVEGKIVLCSSLAGLDTGVEAGAFGSIIQDDQ--NVTHVVPFPATILDTNTYLLAHKYVT 442

Query: 448 SVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPDVSAPGVEILAAFPPVLLP 507
           S  +P A IL+S+ I + ++P    FSSRGPNA++PEIMKPD++APG+EILAA+PP L P
Sbjct: 443 STTSPLAEILRSETINNTNSPSVTDFSSRGPNAIVPEIMKPDITAPGLEILAAYPPNLPP 502

Query: 508 ADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPSAIKSAIMTTAKPITSNDG 567
           +   G K+   Y+ +SGTSM+CPH A +AAYVKSFH DWSP+AIKS+IMTT  P+  ++ 
Sbjct: 503 SGKYGDKRSSKYTFLSGTSMACPHVAAIAAYVKSFHPDWSPAAIKSSIMTTTTPLKDSED 562

Query: 568 TIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGYDSKDIQLMSGDFNSSC-SS 627
               E+ +GSG +NP +A+ PGLV++ SK+DYINLLC  GYD+  I+ +SG+ NS+C SS
Sbjct: 563 K---EYAYGSGLVNPVKAINPGLVFDLSKDDYINLLCNIGYDTPKIRKISGE-NSTCPSS 622

Query: 628 NVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNVGDGSSSYKAEFLPFSKVYLTESTE 687
                  +D NYPA+A  V P Q FV  F R VTNVG  +S+YK             ++ 
Sbjct: 623 TPQKSVVKDFNYPALATHVQPDQPFVFNFTRTVTNVGFANSTYKVS---------VPTSS 682

Query: 688 KLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTALVWSDGIHKVRSPIVVEVKS 747
            LN +V PQ +SF ++NEK+SF V V GG  PDG+V S++L W+DG H VR PIVV+V  
Sbjct: 683 DLNITVVPQVISFKSLNEKQSFVVKVVGGKFPDGSVPSSSLQWTDGTHNVRCPIVVDVSR 725

BLAST of Lag0010278 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 704.1 bits (1816), Expect = 1.2e-202
Identity = 374/689 (54.28%), Postives = 473/689 (68.65%), Query Frame = 0

Query: 54  STHLNLLESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELK 113
           S HL++L+ +VGT +   HL+VRSYKRSFNGFAA L+ +E +KL  M  V+SVFPSK  +
Sbjct: 13  SHHLSILQKLVGTIA-ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHE 72

Query: 114 PQTTRSWDFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAG 173
             TTRSWDFVG  +KA+R  V ESD+I+G  D+GIWPESESF+D G GPPP++WKG+C G
Sbjct: 73  LTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKG 132

Query: 174 GANFTCNKKLIGARYYSA-SSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVP 233
           G  F CN KLIGAR+Y+  + SARD  GHGTHTASTAAG+ V  A F+GLA G ARG VP
Sbjct: 133 GLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVP 192

Query: 234 SARIAAYKVCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMT 293
           SARIAAYKVC +RC++ D+LAAFDDAIADGVD+I+IS+     S     ++AIGSFHAM 
Sbjct: 193 SARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMM 252

Query: 294 KGILTVQSAGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPP 353
           +GI+T  SAGN+GP   ++ + +PW+ T+ A+ TDR  +DRVVLGNGK +TG +VN+F  
Sbjct: 253 RGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNL 312

Query: 354 NPNVLPLIYATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAI 413
           N    P++Y  N SR C ++Q+  C  GC+DSELVKGKIV C  + G  EA  AGA G I
Sbjct: 313 NGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVI 372

Query: 414 VLNDKLVNVSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFS 473
           V N  L + + V PFP +++   DY  + +Y  S + P A IL+++ I D  AP   +FS
Sbjct: 373 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 432

Query: 474 SRGPNAVMPEIMKPDVSAPGVEILAAFPPVLLPADL--PGFKQFVNYSIMSGTSMSCPHA 533
           SRGP+ V+  ++KPDVSAPG+EILAAF PV  P+    P  K+ V YS+MSGTSM+CPH 
Sbjct: 433 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 492

Query: 534 AGVAAYVKSFHRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVY 593
           AGVAAYVKSFH DWSPSAIKSAIMTTA P+         EF +GSGQINP +A  PGLVY
Sbjct: 493 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNP-EQEFAYGSGQINPTKASDPGLVY 552

Query: 594 ETSKEDYINLLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFV 653
           E   EDY+ +LC  G+DS  +   SG  N +CS        +DLNYP M   V     F 
Sbjct: 553 EVETEDYLKMLCAEGFDSTTLTTTSGQ-NVTCSERT---EVKDLNYPTMTTFVSSLDPFN 612

Query: 654 VKFVRIVTNVGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTV 713
           V F R VTNVG  +S+YKA  +P           +L  S+EP+ L FG + EKKSF VT+
Sbjct: 613 VTFKRTVTNVGFPNSTYKASVVPLQ--------PELQISIEPEILRFGFLEEKKSFVVTI 672

Query: 714 AGGLIPDGTVFSTALVWSDGIHKVRSPIV 740
           +G  + DG+  S+++VWSDG H VRSPIV
Sbjct: 673 SGKELKDGSFVSSSVVWSDGSHSVRSPIV 687

BLAST of Lag0010278 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 666.8 bits (1719), Expect = 2.1e-191
Identity = 365/744 (49.06%), Postives = 509/744 (68.41%), Query Frame = 0

Query: 10  IQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASE 69
           I L ++LL    SS   D  +  ++++ IVY+GS+ +R      S H+++L+ + G  S 
Sbjct: 8   IFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITG-ESL 67

Query: 70  TEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ-- 129
            E+ +VRSYK+SFNGFAA+LT+SE ++LA M  V+SVFPS++LK QTT SW+F+GL++  
Sbjct: 68  IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 127

Query: 130 KAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGAR 189
           K KR    ESD IIG  D+GI+PES+SF+D G GPPP++WKGTCAGG NFTCN K+IGAR
Sbjct: 128 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 187

Query: 190 YYSASS----SARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE 249
            Y+A S    +ARD +GHGTHTAS AAG+ VA + F+GL  G ARG VP+ARIA YKVC+
Sbjct: 188 DYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCD 247

Query: 250 SR-CHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAG 309
           +  C    +++AFDDAIADGVD+I+IS+       F+ D IAIG+FHAM  G+LTV +AG
Sbjct: 248 NEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAG 307

Query: 310 NSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYA 369
           N+GP  ST+TS+APW+F++ A+ T+R+ + +VVLG+GK + G +VN++  N    PL+Y 
Sbjct: 308 NNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYG 367

Query: 370 TNAS-RTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 429
            +A+  TC   ++  C+P CLD +LVKGKIV C +  G  EA   GA G+IV N +  + 
Sbjct: 368 KSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE-PDR 427

Query: 430 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 489
           + +  FP++ ++  DY  + +Y  S +NP A +LKS+ I +  AP  A+FSSRGP++++ 
Sbjct: 428 AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVS 487

Query: 490 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 549
           +I+KPD++APGVEILAA+ P   P +     + V YS++SGTSM+CPH AGVAAYVK+FH
Sbjct: 488 DILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFH 547

Query: 550 RDWSPSAIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 609
             WSPS I+SAIMTTA P+  S  G +  EF +GSG ++P +A+ PGLVYE +K D+IN 
Sbjct: 548 PQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINF 607

Query: 610 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 669
           LCG  Y S  ++++SGD NS+C+  ++    R+LNYP M+A+V  T+ F + F R VTNV
Sbjct: 608 LCGLNYTSDHLRIISGD-NSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 667

Query: 670 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 729
           G   S+Y A+ + F          KL+  V P+ LS  ++NEK+SF VTV+   I     
Sbjct: 668 GMQKSTYNAKVVKF-------PGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 727

Query: 730 FSTALVWSDGIHKVRSPIVVEVKS 745
            S  L+WSDG H VRSPI+V   S
Sbjct: 728 VSANLIWSDGTHNVRSPIIVYAMS 741

BLAST of Lag0010278 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 663.7 bits (1711), Expect = 1.8e-190
Identity = 367/741 (49.53%), Postives = 494/741 (66.67%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ + A +  S      + +++++ IVYMG++ +R      S H ++L+ V G +S  +
Sbjct: 9   LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
            L VR+YKRSFNGFAA+LT+SE E LA+M+ V+SVFPSK L  QTT SW+F+GL++  + 
Sbjct: 69  RL-VRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 128

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN + ESD IIG  D+GI+PES+SF+  G GPPP++WKG C GG NFTCN KLIGARYY
Sbjct: 129 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY 188

Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCES 251
           +        SARD+TGHG+HTAS AAG+ V    F+GL  G  RG VP+ARIA YKVC+ 
Sbjct: 189 TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDP 248

Query: 252 ---RCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
              RC    +LAAFDDAIAD VD+IT+S+G      F+ D +AIG+FHAM KGILTV  A
Sbjct: 249 GVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGA 308

Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
           GN+GP   T+ S APWLFT+ A+  +R+ + +VVLGNGKT+ G +VNSF  N    PL+Y
Sbjct: 309 GNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY 368

Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
             +AS  C  S +  C PGCLDS+ VKGKIV C T     EA   GA  +IV N    + 
Sbjct: 369 GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDA 428

Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
           + V  FP++ ++  DY  V +Y  S +NP A +LKS+ I +  AP  A++SSRGPN ++ 
Sbjct: 429 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 488

Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
           +I+KPD++APG EILAA+ P + P++     + V Y+++SGTSMSCPH AGVAAY+K+FH
Sbjct: 489 DILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 548

Query: 552 RDWSPSAIKSAIMTTAKPI--TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
             WSPS I+SAIMTTA P+  +++    + EF +G+G ++P  A+ PGLVYE +K D+I 
Sbjct: 549 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 608

Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
            LCG  Y  K ++L+SGD +SSC+   T    R+LNYP+M+AQV  T+ F V F R VTN
Sbjct: 609 FLCGFNYTGKKLRLISGD-SSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTN 668

Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
           VG  +++YKA+ +            KL   V P  LS  ++ EKKSFTVTV+G       
Sbjct: 669 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 728

Query: 732 VFSTALVWSDGIHKVRSPIVV 741
           + S  L+WSDG+H VRSPIVV
Sbjct: 729 LVSAQLIWSDGVHFVRSPIVV 734

BLAST of Lag0010278 vs. TAIR 10
Match: AT3G46840.1 (Subtilase family protein )

HSP 1 Score: 650.2 bits (1676), Expect = 2.0e-186
Identity = 369/741 (49.80%), Postives = 492/741 (66.40%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ + A +  S      + ++++  IVYMG++  R      S H ++L+ V G +S  E
Sbjct: 10  LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESS-IE 69

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
             +VR+YKRSFNGFAA+LT SE E LA+M+ V+SVFP+K+LK QTT SW+F+GL++  + 
Sbjct: 70  DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN + ESD IIG  D+GI+PES+SF+  G GPPP++WKG C GG NFT N KLIGARYY
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189

Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE- 251
           +        SARD  GHG+HTASTAAG+ V    F+GL  G ARG VP+ARIA YKVC+ 
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249

Query: 252 --SRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
               C    +LAAFDDAIAD VD+ITIS+GG   S F+ D IAIG+FHAM KGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309

Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
           GNSGP  ST+ S APW+FT+ A+ T+R+ V +VVLGNGKTV G +VNSF  N    PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369

Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
             +AS +C  + +  C PGCLDS+ VKGKIV C +     EA   GA  +IV + +  +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR-TDV 429

Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
           + +  FP++ +   DY  V +Y  S +NP A +LKS+ I +  AP  A++ SRGPN ++P
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489

Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
           +I+KPD++APG EI+AA+ P   P+      + V YS+ +GTSMSCPH AGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549

Query: 552 RDWSPSAIKSAIMTTAKPITSNDGTI--IGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
             WSPS I+SAIMTTA P+ ++      + EF +G+G ++P  A+ PGLVYE +K D+I 
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609

Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
            LCG  Y +K+++L+SGD +SSC+   T    R+LNYP+M AQV   + F V F R VTN
Sbjct: 610 FLCGLNYTAKNLRLISGD-SSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTN 669

Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
           VG  +++YKA+ +            KL   V P  LS  ++ EKKSFTVT +G       
Sbjct: 670 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAEN 729

Query: 732 VFSTALVWSDGIHKVRSPIVV 741
           + S  L+WSDG+H VRSPIVV
Sbjct: 730 LVSAQLIWSDGVHFVRSPIVV 734

BLAST of Lag0010278 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 636.0 bits (1639), Expect = 3.9e-182
Identity = 364/735 (49.52%), Postives = 482/735 (65.58%), Query Frame = 0

Query: 12  LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
           L++ LL    SS    S    ++++ IVYMGS+ +R     +S H+N+L+ V G +S  E
Sbjct: 10  LLSCLLVLFLSSV---SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESS-IE 69

Query: 72  HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
             +VRSYKRSFNGFAA+LT+SE E++A M GV+SVFP+K+L+ QTT SWDF+GL++  K 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKT 129

Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
           KRN   ESD IIG  D+GI PES+SF+D G GPPPQ+WKG C+GG NFTCN KLIGAR Y
Sbjct: 130 KRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 192 SASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHE 251
           + S   RD  GHGTHTASTAAG+ V  A FFG+  G  RG VP++R+AAYKVC  + C  
Sbjct: 190 T-SEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSS 249

Query: 252 ADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAA 311
             +L+AFDDAIADGVDLITIS+G    S F+ D IAIG+FHAM KG+LTV SAGNSGP  
Sbjct: 250 EALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKP 309

Query: 312 STLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATN-ASR 371
            +++  APW+ T+ A+TT+R  V +VVLGNGKT+ G +VN++       PL+Y  + AS 
Sbjct: 310 ISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASS 369

Query: 372 TCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPF 431
            C    +  C+  C+D   VKGKI+ C    G     + GA G I    K  +V+ + P 
Sbjct: 370 ACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPK-PDVAFIHPL 429

Query: 432 PMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPD 491
           P   +   D+  + +Y  S  +P A +LK++AI + ++P  A+FSSRGPN +  +I+KPD
Sbjct: 430 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 489

Query: 492 VSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPS 551
           ++APGVEILAA+ P   P+      + V YS++SGTSMSCPH AGVAAYVK+F+  WSPS
Sbjct: 490 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 549

Query: 552 AIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGY 611
            I+SAIMTTA P+  +  G    EF +GSG ++P  A  PGLVYE  K D+I  LCG  Y
Sbjct: 550 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 609

Query: 612 DSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPT-QAFVVKFVRIVTNVGDGSS 671
            S+ ++++SG+   +CS      P R+LNYP+M+A++  +   F V F R +TNVG  +S
Sbjct: 610 TSQVLKVISGE-TVTCSEAKKILP-RNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNS 669

Query: 672 SYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTAL 731
           +Y       SKV     + KL+  + P  LSF  VNEK+SFTVTV G  +      S  L
Sbjct: 670 TYT------SKVVAGHGS-KLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 727

Query: 732 VWSDGIHKVRSPIVV 741
           +WSDG H VRSPIVV
Sbjct: 730 IWSDGTHNVRSPIVV 727

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022958362.19.2e-25863.78subtilisin-like protease SBT4.3 [Cucurbita moschata][more]
KAG6605933.12.1e-25763.38Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023533940.12.4e-25362.45subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo][more]
XP_022995032.12.6e-25262.45subtilisin-like protease SBT4.3 [Cucurbita maxima][more]
XP_022995031.11.9e-25062.45subtilisin-like protease SBT4.3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FIF82.0e-20251.89Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FGU32.9e-19049.06Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... [more]
Q9STF72.5e-18949.53Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
F4JA912.8e-18549.80Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... [more]
Q9FIG25.6e-18149.52Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1H1M34.5e-25863.78subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE... [more]
A0A6J1JXM01.3e-25262.45subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3... [more]
A0A6J1K0V89.0e-25162.45subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3... [more]
A0A6J1H1W01.0e-24661.10subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE... [more]
A0A4P1R6C93.8e-20954.22Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_29596 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT5G59190.11.2e-20254.28subtilase family protein [more]
AT5G59100.12.1e-19149.06Subtilisin-like serine endopeptidase family protein [more]
AT3G46850.11.8e-19049.53Subtilase family protein [more]
AT3G46840.12.0e-18649.80Subtilase family protein [more]
AT5G59120.13.9e-18249.52subtilase 4.13 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 521..537
score: 57.4
coord: 136..155
score: 23.47
coord: 197..210
score: 49.97
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 633..740
e-value: 2.4E-22
score: 79.0
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 38..112
e-value: 2.1E-10
score: 41.1
NoneNo IPR availableGENE3D3.50.30.30coord: 327..461
e-value: 4.7E-164
score: 548.6
NoneNo IPR availableGENE3D2.60.40.2310coord: 601..743
e-value: 1.8E-33
score: 117.1
NoneNo IPR availablePANTHERPTHR10795:SF685SUBTILISIN-LIKE PROTEASE SBT4.3coord: 37..743
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 119..584
score: 28.130219
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 334..454
e-value: 6.65918E-19
score: 81.3059
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 20..114
e-value: 3.8E-19
score: 70.9
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 138..597
e-value: 4.7E-164
score: 548.6
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 115..588
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 137..561
e-value: 3.8E-41
score: 141.2
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 37..743
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 522..532
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 112..557
e-value: 1.39343E-118
score: 356.908

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0010278.1Lag0010278.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity