Homology
BLAST of Lag0010278 vs. NCBI nr
Match:
XP_022958362.1 (subtilisin-like protease SBT4.3 [Cucurbita moschata])
HSP 1 Score: 900.6 bits (2326), Expect = 9.2e-258
Identity = 479/751 (63.78%), Postives = 574/751 (76.43%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA IN PI+ +AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAAINFPILFTFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS SSSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P TA+N Y VANYA S NP RILKS AIKD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
PEIMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE ++DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + FVVKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F +NEKKSF VTVAGG IP T
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS AL+WSDGIHKVRSPIVV VK IPT+
Sbjct: 721 FSLALIWSDGIHKVRSPIVVNVK-LPPIPTS 738
BLAST of Lag0010278 vs. NCBI nr
Match:
KAG6605933.1 (Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 899.4 bits (2323), Expect = 2.1e-257
Identity = 476/751 (63.38%), Postives = 571/751 (76.03%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI +AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAANNFPIFFSFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS SSSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P A+N Y V NYA S NP RILKS AIKD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVTNYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
PEIMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE ++DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + FVVKF RIVTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRIVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F +NEKKSF VTVAGG IP T
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPTELTFKDLNEKKSFVVTVAGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS+AL+WSDGIHKVRSPIVV VK T +N
Sbjct: 721 FSSALIWSDGIHKVRSPIVVNVKLPPTPTSN 739
BLAST of Lag0010278 vs. NCBI nr
Match:
XP_023533940.1 (subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 885.9 bits (2288), Expect = 2.4e-253
Identity = 469/751 (62.45%), Postives = 572/751 (76.17%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI +AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAANNFPISFSFIAALLAAIFT-----SANGSERKDHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
+SV+GT+S TE +RSY RSFNGF A+LT E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61 QSVIGTSSVTEDSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGMGPPPQKWRGECAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS SSSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P A+N+ Y VANYA S NP RIL+S A+KD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNLTAYEQVANYAISEPNPHVRILQSVAVKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
PEIMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQI+PK+A++PGLVYE ++DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFLSGEFLHGSGQIDPKQAIEPGLVYEIFEDDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + F VKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVPPMKPFAVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF P S VY+ +S+EKLN SVEP EL+F +NEKKSF VTVAGG IP T
Sbjct: 661 GIANSTYKSEFSPLSVVYVLKSSEKLNVSVEPPELTFRDLNEKKSFVVTVAGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS+AL+WSDGIHKVRSP+V+ VK IPT+
Sbjct: 721 FSSALIWSDGIHKVRSPMVLNVK-LPPIPTS 738
BLAST of Lag0010278 vs. NCBI nr
Match:
XP_022995032.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])
HSP 1 Score: 882.5 bits (2279), Expect = 2.6e-252
Identity = 469/751 (62.45%), Postives = 567/751 (75.50%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI+ L+AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAPNNFPILFSLIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT +E E+LA M+GV+SVF SKELK QTTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS +SSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-TSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM +GILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSI+D VLGNGK V+GY++N F PN NV LI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNV-TLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P A+N Y VANYA S NP RILKS AIKD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
P+IMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PDIMKPDIAAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQINPK+A++PGLVYE + DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + FV+KF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF FS VY+ +S+EK N SVEP EL+F +NEKKSF VTV GG IP T
Sbjct: 661 GIANSTYKSEFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS+ALVWSDGIHKVRSPIVV VK IPT+
Sbjct: 721 FSSALVWSDGIHKVRSPIVVNVK-LPPIPTS 738
BLAST of Lag0010278 vs. NCBI nr
Match:
XP_022995031.1 (subtilisin-like protease SBT4.3 [Cucurbita maxima])
HSP 1 Score: 876.3 bits (2263), Expect = 1.9e-250
Identity = 464/743 (62.45%), Postives = 556/743 (74.83%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI+ +AALLAA+ S S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAPDNFPILFSFIAALLAAILS-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT +E E+LAAM+GV+SVF SK K QTTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
D++G K RN+ E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTCAGG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGAR+Y+ S SARD+ GHG+HTASTAAG F+GLA G ARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYK 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
VC C E ++LAAFDDAIADGVDLITIS+GG GG F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 VCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP T+ S PW+FT+ ATTTDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
YATNASR C +E C GCLD LVKGKIVQC ++GAS A NAGAAGAIVLND N
Sbjct: 361 YATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS V PFP A+ + DY VANYA S NP I +S A KD AP A FSSRGPN M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
EI+KPD++APGVEILA+F P+ P+ G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSP+AIKSAIMTTAK I DG +I EFL+GSG I+P A++PGLVYE ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNV 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LC GYDSK ++ +G+ +S CS T F ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMEAFAGN-DSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+Y+++ L FS V + +S EKLN SV+PQEL F +NEKKSF VTV GG I V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
FS+AL+WSD H+VRSPIVV +K
Sbjct: 721 FSSALIWSDLDHEVRSPIVVMIK 734
BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 707.2 bits (1824), Expect = 2.0e-202
Identity = 385/742 (51.89%), Postives = 497/742 (66.98%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MAK++ P+ + +A + S+ + + + IVYMG++ + S HL++L
Sbjct: 1 MAKLSTPLYLICLAFIFTRDVSA----NDYRQASSVYIVYMGTLPEIKYSP-PSHHLSIL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
+ +VGT + HL+VRSYKRSFNGFAA L+ +E +KL M V+SVFPSK + TTRSW
Sbjct: 61 QKLVGTIA-ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DFVG +KA+R V ESD+I+G D+GIWPESESF+D G GPPP++WKG+C GG F CN
Sbjct: 121 DFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACN 180
Query: 181 KKLIGARYYSA-SSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAY 240
KLIGAR+Y+ + SARD GHGTHTASTAAG+ V A F+GLA G ARG VPSARIAAY
Sbjct: 181 NKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAY 240
Query: 241 KVCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQ 300
KVC +RC++ D+LAAFDDAIADGVD+I+IS+ S ++AIGSFHAM +GI+T
Sbjct: 241 KVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAG 300
Query: 301 SAGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPL 360
SAGN+GP ++ + +PW+ T+ A+ TDR +DRVVLGNGK +TG +VN+F N P+
Sbjct: 301 SAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPI 360
Query: 361 IYATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLV 420
+Y N SR C ++Q+ C GC+DSELVKGKIV C + G EA AGA G IV N L
Sbjct: 361 VYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLP 420
Query: 421 NVSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAV 480
+ + V PFP +++ DY + +Y S + P A IL+++ I D AP +FSSRGP+ V
Sbjct: 421 DSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFV 480
Query: 481 MPEIMKPDVSAPGVEILAAFPPVLLPADL--PGFKQFVNYSIMSGTSMSCPHAAGVAAYV 540
+ ++KPDVSAPG+EILAAF PV P+ P K+ V YS+MSGTSM+CPH AGVAAYV
Sbjct: 481 IQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYV 540
Query: 541 KSFHRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDY 600
KSFH DWSPSAIKSAIMTTA P+ EF +GSGQINP +A PGLVYE EDY
Sbjct: 541 KSFHPDWSPSAIKSAIMTTATPMNLKKNP-EQEFAYGSGQINPTKASDPGLVYEVETEDY 600
Query: 601 INLLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIV 660
+ +LC G+DS + SG N +CS +DLNYP M V F V F R V
Sbjct: 601 LKMLCAEGFDSTTLTTTSGQ-NVTCSERT---EVKDLNYPTMTTFVSSLDPFNVTFKRTV 660
Query: 661 TNVGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPD 720
TNVG +S+YKA +P +L S+EP+ L FG + EKKSF VT++G + D
Sbjct: 661 TNVGFPNSTYKASVVPLQ--------PELQISIEPEILRFGFLEEKKSFVVTISGKELKD 720
Query: 721 GTVFSTALVWSDGIHKVRSPIV 740
G+ S+++VWSDG H VRSPIV
Sbjct: 721 GSFVSSSVVWSDGSHSVRSPIV 723
BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 666.8 bits (1719), Expect = 2.9e-190
Identity = 365/744 (49.06%), Postives = 509/744 (68.41%), Query Frame = 0
Query: 10 IQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASE 69
I L ++LL SS D + ++++ IVY+GS+ +R S H+++L+ + G S
Sbjct: 8 IFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITG-ESL 67
Query: 70 TEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ-- 129
E+ +VRSYK+SFNGFAA+LT+SE ++LA M V+SVFPS++LK QTT SW+F+GL++
Sbjct: 68 IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 127
Query: 130 KAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGAR 189
K KR ESD IIG D+GI+PES+SF+D G GPPP++WKGTCAGG NFTCN K+IGAR
Sbjct: 128 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 187
Query: 190 YYSASS----SARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE 249
Y+A S +ARD +GHGTHTAS AAG+ VA + F+GL G ARG VP+ARIA YKVC+
Sbjct: 188 DYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCD 247
Query: 250 SR-CHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAG 309
+ C +++AFDDAIADGVD+I+IS+ F+ D IAIG+FHAM G+LTV +AG
Sbjct: 248 NEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAG 307
Query: 310 NSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYA 369
N+GP ST+TS+APW+F++ A+ T+R+ + +VVLG+GK + G +VN++ N PL+Y
Sbjct: 308 NNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYG 367
Query: 370 TNAS-RTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 429
+A+ TC ++ C+P CLD +LVKGKIV C + G EA GA G+IV N + +
Sbjct: 368 KSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE-PDR 427
Query: 430 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 489
+ + FP++ ++ DY + +Y S +NP A +LKS+ I + AP A+FSSRGP++++
Sbjct: 428 AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVS 487
Query: 490 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 549
+I+KPD++APGVEILAA+ P P + + V YS++SGTSM+CPH AGVAAYVK+FH
Sbjct: 488 DILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFH 547
Query: 550 RDWSPSAIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 609
WSPS I+SAIMTTA P+ S G + EF +GSG ++P +A+ PGLVYE +K D+IN
Sbjct: 548 PQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINF 607
Query: 610 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 669
LCG Y S ++++SGD NS+C+ ++ R+LNYP M+A+V T+ F + F R VTNV
Sbjct: 608 LCGLNYTSDHLRIISGD-NSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 667
Query: 670 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 729
G S+Y A+ + F KL+ V P+ LS ++NEK+SF VTV+ I
Sbjct: 668 GMQKSTYNAKVVKF-------PGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 727
Query: 730 FSTALVWSDGIHKVRSPIVVEVKS 745
S L+WSDG H VRSPI+V S
Sbjct: 728 VSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 663.7 bits (1711), Expect = 2.5e-189
Identity = 367/741 (49.53%), Postives = 494/741 (66.67%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ + A + S + +++++ IVYMG++ +R S H ++L+ V G +S +
Sbjct: 9 LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
L VR+YKRSFNGFAA+LT+SE E LA+M+ V+SVFPSK L QTT SW+F+GL++ +
Sbjct: 69 RL-VRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 128
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN + ESD IIG D+GI+PES+SF+ G GPPP++WKG C GG NFTCN KLIGARYY
Sbjct: 129 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY 188
Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCES 251
+ SARD+TGHG+HTAS AAG+ V F+GL G RG VP+ARIA YKVC+
Sbjct: 189 TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDP 248
Query: 252 ---RCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
RC +LAAFDDAIAD VD+IT+S+G F+ D +AIG+FHAM KGILTV A
Sbjct: 249 GVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGA 308
Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
GN+GP T+ S APWLFT+ A+ +R+ + +VVLGNGKT+ G +VNSF N PL+Y
Sbjct: 309 GNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY 368
Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
+AS C S + C PGCLDS+ VKGKIV C T EA GA +IV N +
Sbjct: 369 GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDA 428
Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
+ V FP++ ++ DY V +Y S +NP A +LKS+ I + AP A++SSRGPN ++
Sbjct: 429 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 488
Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
+I+KPD++APG EILAA+ P + P++ + V Y+++SGTSMSCPH AGVAAY+K+FH
Sbjct: 489 DILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 548
Query: 552 RDWSPSAIKSAIMTTAKPI--TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
WSPS I+SAIMTTA P+ +++ + EF +G+G ++P A+ PGLVYE +K D+I
Sbjct: 549 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 608
Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
LCG Y K ++L+SGD +SSC+ T R+LNYP+M+AQV T+ F V F R VTN
Sbjct: 609 FLCGFNYTGKKLRLISGD-SSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTN 668
Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
VG +++YKA+ + KL V P LS ++ EKKSFTVTV+G
Sbjct: 669 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 728
Query: 732 VFSTALVWSDGIHKVRSPIVV 741
+ S L+WSDG+H VRSPIVV
Sbjct: 729 LVSAQLIWSDGVHFVRSPIVV 734
BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match:
F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)
HSP 1 Score: 650.2 bits (1676), Expect = 2.8e-185
Identity = 369/741 (49.80%), Postives = 492/741 (66.40%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ + A + S + ++++ IVYMG++ R S H ++L+ V G +S E
Sbjct: 10 LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESS-IE 69
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
+VR+YKRSFNGFAA+LT SE E LA+M+ V+SVFP+K+LK QTT SW+F+GL++ +
Sbjct: 70 DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN + ESD IIG D+GI+PES+SF+ G GPPP++WKG C GG NFT N KLIGARYY
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189
Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE- 251
+ SARD GHG+HTASTAAG+ V F+GL G ARG VP+ARIA YKVC+
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249
Query: 252 --SRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
C +LAAFDDAIAD VD+ITIS+GG S F+ D IAIG+FHAM KGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309
Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
GNSGP ST+ S APW+FT+ A+ T+R+ V +VVLGNGKTV G +VNSF N PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369
Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
+AS +C + + C PGCLDS+ VKGKIV C + EA GA +IV + + +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR-TDV 429
Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
+ + FP++ + DY V +Y S +NP A +LKS+ I + AP A++ SRGPN ++P
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489
Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
+I+KPD++APG EI+AA+ P P+ + V YS+ +GTSMSCPH AGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549
Query: 552 RDWSPSAIKSAIMTTAKPITSNDGTI--IGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
WSPS I+SAIMTTA P+ ++ + EF +G+G ++P A+ PGLVYE +K D+I
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609
Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
LCG Y +K+++L+SGD +SSC+ T R+LNYP+M AQV + F V F R VTN
Sbjct: 610 FLCGLNYTAKNLRLISGD-SSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTN 669
Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
VG +++YKA+ + KL V P LS ++ EKKSFTVT +G
Sbjct: 670 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAEN 729
Query: 732 VFSTALVWSDGIHKVRSPIVV 741
+ S L+WSDG+H VRSPIVV
Sbjct: 730 LVSAQLIWSDGVHFVRSPIVV 734
BLAST of Lag0010278 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 636.0 bits (1639), Expect = 5.6e-181
Identity = 364/735 (49.52%), Postives = 482/735 (65.58%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ LL SS S ++++ IVYMGS+ +R +S H+N+L+ V G +S E
Sbjct: 10 LLSCLLVLFLSSV---SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESS-IE 69
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
+VRSYKRSFNGFAA+LT+SE E++A M GV+SVFP+K+L+ QTT SWDF+GL++ K
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKT 129
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN ESD IIG D+GI PES+SF+D G GPPPQ+WKG C+GG NFTCN KLIGAR Y
Sbjct: 130 KRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 192 SASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHE 251
+ S RD GHGTHTASTAAG+ V A FFG+ G RG VP++R+AAYKVC + C
Sbjct: 190 T-SEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSS 249
Query: 252 ADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAA 311
+L+AFDDAIADGVDLITIS+G S F+ D IAIG+FHAM KG+LTV SAGNSGP
Sbjct: 250 EALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKP 309
Query: 312 STLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATN-ASR 371
+++ APW+ T+ A+TT+R V +VVLGNGKT+ G +VN++ PL+Y + AS
Sbjct: 310 ISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASS 369
Query: 372 TCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPF 431
C + C+ C+D VKGKI+ C G + GA G I K +V+ + P
Sbjct: 370 ACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPK-PDVAFIHPL 429
Query: 432 PMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPD 491
P + D+ + +Y S +P A +LK++AI + ++P A+FSSRGPN + +I+KPD
Sbjct: 430 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 489
Query: 492 VSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPS 551
++APGVEILAA+ P P+ + V YS++SGTSMSCPH AGVAAYVK+F+ WSPS
Sbjct: 490 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 549
Query: 552 AIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGY 611
I+SAIMTTA P+ + G EF +GSG ++P A PGLVYE K D+I LCG Y
Sbjct: 550 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 609
Query: 612 DSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPT-QAFVVKFVRIVTNVGDGSS 671
S+ ++++SG+ +CS P R+LNYP+M+A++ + F V F R +TNVG +S
Sbjct: 610 TSQVLKVISGE-TVTCSEAKKILP-RNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNS 669
Query: 672 SYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTAL 731
+Y SKV + KL+ + P LSF VNEK+SFTVTV G + S L
Sbjct: 670 TYT------SKVVAGHGS-KLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 727
Query: 732 VWSDGIHKVRSPIVV 741
+WSDG H VRSPIVV
Sbjct: 730 IWSDGTHNVRSPIVV 727
BLAST of Lag0010278 vs. ExPASy TrEMBL
Match:
A0A6J1H1M3 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE=3 SV=1)
HSP 1 Score: 900.6 bits (2326), Expect = 4.5e-258
Identity = 479/751 (63.78%), Postives = 574/751 (76.43%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA IN PI+ +AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAAINFPILFTFIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT E E+LAAM+GV+SVF SKELK +TTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAELTGGEAEQLAAMDGVVSVFESKELKTKTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLSGELDVIIGSIDSGIWPESESFNDDGIGPPPQKWRGVCAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS SSSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-SSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM KGILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSIVD +VLGNGKTV+GY++N F PN +V PLI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIVDEIVLGNGKTVSGYSINPFTPNKDV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFAGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P TA+N Y VANYA S NP RILKS AIKD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPATALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
PEIMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PEIMKPDIAAPGVEILASVNPQPPNSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ IT+ DG + GEFLHGSGQINPK+A++PGLVYE ++DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEITNTDGFLSGEFLHGSGQINPKQAIEPGLVYEIFEDDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + FVVKF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSRFSTKFSPQDLNYPAMVFEVPPMKPFVVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF PFS VY+ +S+EKLN SVEP EL+F +NEKKSF VTVAGG IP T
Sbjct: 661 GTANSTYKSEFSPFSIVYVLKSSEKLNVSVEPPELTFKDLNEKKSFVVTVAGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS AL+WSDGIHKVRSPIVV VK IPT+
Sbjct: 721 FSLALIWSDGIHKVRSPIVVNVK-LPPIPTS 738
BLAST of Lag0010278 vs. ExPASy TrEMBL
Match:
A0A6J1JXM0 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3 SV=1)
HSP 1 Score: 882.5 bits (2279), Expect = 1.3e-252
Identity = 469/751 (62.45%), Postives = 567/751 (75.50%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI+ L+AALLAA+ + S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAPNNFPILFSLIAALLAAIFT-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT +E E+LA M+GV+SVF SKELK QTTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLATMDGVVSVFESKELKTQTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
DF+G Q+ KRN+ E D+IIGS D+GIWPESESFND G+GPPPQ+W+G CAGG NFTCN
Sbjct: 121 DFLGF-QQPKRNLAGELDVIIGSIDSGIWPESESFNDEGIGPPPQKWRGVCAGGENFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGARYYS +SSARD GHGTHTASTA G V F+G+A GVARGAVPS+R+A Y
Sbjct: 181 HKVIGARYYS-TSSARDDKGHGTHTASTAIGKSVTTDGFYGIAGGVARGAVPSSRLAVYS 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C + ++LAAFDDAIADGVD+ITIS+GG S + D IAIG++HAM +GILTVQS
Sbjct: 241 ACSPSCTDVEILAAFDDAIADGVDIITISLGGFDLSDLRVDCIAIGAYHAMVRGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP ++ S APWLF++ ATTTDRSI+D VLGNGK V+GY++N F PN NV LI
Sbjct: 301 AGNDGPVVGSVESVAPWLFSVAATTTDRSIIDETVLGNGKIVSGYSINPFTPNKNV-TLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
Y TNAS++C E C CLD +LVKGKIVQC ++ G +A++AGAAGAIVLNDK N
Sbjct: 361 YGTNASKSCSFQNPEVC--SCLDPKLVKGKIVQCKSFTGIFKAVDAGAAGAIVLNDKADN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS + P P A+N Y VANYA S NP RILKS AIKD AP AA FSSRGPN ++
Sbjct: 421 VSFIVPLPAIALNFTAYEQVANYAISEPNPHVRILKSVAIKDGYAPMAAQFSSRGPNLLL 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
P+IMKPD++APGVEILA+ P +D PG + VN++IMSGTSM+CPH AG+AAYVKSF
Sbjct: 481 PDIMKPDIAAPGVEILASVNPQPPQSDTPGDNRTVNFTIMSGTSMACPHVAGLAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSPSAIKSAIMTTA+ +T+ DG + GEFLHGSGQINPK+A++PGLVYE + DY+
Sbjct: 541 HPNWSPSAIKSAIMTTAEEMTNTDGFVSGEFLHGSGQINPKQAIEPGLVYEIFEVDYLKF 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LCGNG+DSK ++ +SG+ S CS T F +DLNYPAM +V P + FV+KF R VTNV
Sbjct: 601 LCGNGFDSKSVRGISGN-KSDCSKFSTKFSPQDLNYPAMVFEVSPMKPFVIKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+YK+EF FS VY+ +S+EK N SVEP EL+F +NEKKSF VTV GG IP T
Sbjct: 661 GIANSTYKSEFSAFSVVYVLKSSEKPNVSVEPPELTFRDLNEKKSFIVTVTGGSIPARTG 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVKSQSTIPTN 752
FS+ALVWSDGIHKVRSPIVV VK IPT+
Sbjct: 721 FSSALVWSDGIHKVRSPIVVNVK-LPPIPTS 738
BLAST of Lag0010278 vs. ExPASy TrEMBL
Match:
A0A6J1K0V8 (subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3 SV=1)
HSP 1 Score: 876.3 bits (2263), Expect = 9.0e-251
Identity = 464/743 (62.45%), Postives = 556/743 (74.83%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI+ +AALLAA+ S S N ERK +IVYMG+I NR A+ S+ HLNLL
Sbjct: 1 MAPDNFPILFSFIAALLAAILS-----SANGSERKAHIVYMGAIQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S TE +RSY RSFNGF A+LT +E E+LAAM+GV+SVF SK K QTTRSW
Sbjct: 61 RSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
D++G K RN+ E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTCAGG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGAR+Y+ S SARD+ GHG+HTASTAAG F+GLA G ARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYK 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
VC C E ++LAAFDDAIADGVDLITIS+GG GG F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 VCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP T+ S PW+FT+ ATTTDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
YATNASR C +E C GCLD LVKGKIVQC ++GAS A NAGAAGAIVLND N
Sbjct: 361 YATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS V PFP A+ + DY VANYA S NP I +S A KD AP A FSSRGPN M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
EI+KPD++APGVEILA+F P+ P+ G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSP+AIKSAIMTTAK I DG +I EFL+GSG I+P A++PGLVYE ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNV 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LC GYDSK ++ +G+ +S CS T F ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMEAFAGN-DSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+Y+++ L FS V + +S EKLN SV+PQEL F +NEKKSF VTV GG I V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
FS+AL+WSD H+VRSPIVV +K
Sbjct: 721 FSSALIWSDLDHEVRSPIVVMIK 734
BLAST of Lag0010278 vs. ExPASy TrEMBL
Match:
A0A6J1H1W0 (subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE=3 SV=1)
HSP 1 Score: 862.8 bits (2228), Expect = 1.0e-246
Identity = 454/743 (61.10%), Postives = 552/743 (74.29%), Query Frame = 0
Query: 1 MAKINHPIVIQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLL 60
MA N PI+ +AALLAA+ + S N ERK +IVYMG++ NR A+ S+ HLNLL
Sbjct: 1 MAPENFPILFSFIAALLAAIFT-----SANGSERKAHIVYMGAVQNRAMAE-STHHLNLL 60
Query: 61 ESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSW 120
SV+GT+S E +RSY RSFNGF A+LT E E+LAAM+GV+SVF SK K QTTRSW
Sbjct: 61 RSVIGTSSVMEGSYIRSYGRSFNGFVAKLTGGEAEQLAAMDGVVSVFESKVFKTQTTRSW 120
Query: 121 DFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCN 180
D++G K RN+ E+D+IIGS DTGIWPE ESFND G+GPPP RW+GTC GG NFTCN
Sbjct: 121 DYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCTGGGNFTCN 180
Query: 181 KKLIGARYYSASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYK 240
K+IGAR+Y+ S SARD+ GHG+HTASTAAG F+GLA GVARGAVPS+R+A YK
Sbjct: 181 NKVIGARFYT-SMSARDNVGHGSHTASTAAGKSGKTTGFYGLAGGVARGAVPSSRLAIYK 240
Query: 241 VCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQS 300
C C E ++LAAFDDAIADGVDLITIS+ G+GG F+ D++AIGS+H+M KGILTVQS
Sbjct: 241 ACNPDCLEENILAAFDDAIADGVDLITISIVGAGGFNFEHDSMAIGSYHSMAKGILTVQS 300
Query: 301 AGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLI 360
AGN GP T+ S PW+FT+ AT TDR+IVD+VVLG+G TV GY+VN+F PN NV PLI
Sbjct: 301 AGNYGPEDGTVGSVVPWVFTVAATNTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLI 360
Query: 361 YATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVN 420
YATNASR C +E C+ GCLD LVKGKIVQC ++GAS A NAGAAGAIVLND N
Sbjct: 361 YATNASRNCSSKNAEICRDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 420
Query: 421 VSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVM 480
VS V PFP A+ + DY VANYA S NP I +S A KD AP A FSSRGPN M
Sbjct: 421 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPHAPMIADFSSRGPNMWM 480
Query: 481 PEIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSF 540
EI+KPD++APGVEILA+F P+ P+ G K+ V +SI+SGTSMSCPH AGVAAYVKSF
Sbjct: 481 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 540
Query: 541 HRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 600
H +WSP+AIKSAIMTTAK I DG +I EFL+GSG ++P A++PGLVYE ++D++N+
Sbjct: 541 HPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLVDPDRAIEPGLVYEIFEKDHLNM 600
Query: 601 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 660
LC GYDSK ++ +G+ +S C T + ARDLNYPAM A VLP + FVVKF R VTNV
Sbjct: 601 LCAKGYDSKTMKAFAGN-DSVCPKTWTKYLARDLNYPAMVAHVLPMKPFVVKFQRTVTNV 660
Query: 661 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 720
G +S+Y+++ L FS V + +S EKL+ SV+PQ+L FG +NEKKSF VTV GG IP V
Sbjct: 661 GVANSTYRSKILSFSGVNVLKSLEKLSVSVKPQKLWFGNLNEKKSFVVTVVGGTIPTKIV 720
Query: 721 FSTALVWSDGIHKVRSPIVVEVK 744
S+ L+WSD H+VRSPIVV VK
Sbjct: 721 LSSVLIWSDLDHEVRSPIVVMVK 734
BLAST of Lag0010278 vs. ExPASy TrEMBL
Match:
A0A4P1R6C9 (Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_29596 PE=3 SV=1)
HSP 1 Score: 738.0 bits (1904), Expect = 3.8e-209
Identity = 392/723 (54.22%), Postives = 506/723 (69.99%), Query Frame = 0
Query: 28 SGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETEHLMVRSYKRSFNGFAA 87
+G +RK+ I+YMGS+ + S H+N+L+ V+G ++ HL V SYKRSFNGFAA
Sbjct: 23 NGANEDRKVYIIYMGSLPKENYSP-QSEHINILQQVLGDDFDSNHL-VTSYKRSFNGFAA 82
Query: 88 QLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQKAKRNVVSESDIIIGSFDTG 147
+LTD E + + M GV+S+F SK L+ TTRSWDF+GL++K K + + ESD+IIG FDTG
Sbjct: 83 KLTDQEVKNIGRMRGVVSIFESKTLQLHTTRSWDFIGLQEKTKTSPI-ESDMIIGVFDTG 142
Query: 148 IWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYYSASSSARDSTGHGTHTAS 207
IWPESESF+D G GPPP++WKGTCAGG NFTCN K+IGARYYS + SARDS GHGTHTAS
Sbjct: 143 IWPESESFDDEGFGPPPKKWKGTCAGGKNFTCNNKIIGARYYSENVSARDSVGHGTHTAS 202
Query: 208 TAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHEADVLAAFDDAIADGVDLI 267
TAAG+ V G F+G+A G ARG VPSARIA YKVC +S+C +A +LAAFDDAIADGVDL+
Sbjct: 203 TAAGNKVKGTSFYGIAEGTARGGVPSARIAVYKVCDDSQCSDAAILAAFDDAIADGVDLV 262
Query: 268 TISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAASTLTSSAPWLFTIGATTT 327
TIS+GGS +F D AIGSFHAM KGILTV S GN GP ++ S APWLF++ A+ T
Sbjct: 263 TISIGGSTPRSFTEDPNAIGSFHAMEKGILTVNSGGNGGPIKTSTASLAPWLFSVAASNT 322
Query: 328 DRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATNASR-TCPRSQSERCQPGCLDSE 387
DR I+D+V LGNG T+TG +VN+F PN +PL+ A S+ CP S C P CLD E
Sbjct: 323 DRRIIDKVSLGNGVTLTGQSVNTFTPNGTKIPLVLAEKVSKPNCPIPSS--CFPNCLDPE 382
Query: 388 LVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPFPMTAVNVMDYGDVANYAT 447
V+GKIV C + G + AGA G+I+ +D+ NV+ V PFP T ++ Y Y T
Sbjct: 383 KVEGKIVLCSSLAGLDTGVEAGAFGSIIQDDQ--NVTHVVPFPATILDTNTYLLAHKYVT 442
Query: 448 SVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPDVSAPGVEILAAFPPVLLP 507
S +P A IL+S+ I + ++P FSSRGPNA++PEIMKPD++APG+EILAA+PP L P
Sbjct: 443 STTSPLAEILRSETINNTNSPSVTDFSSRGPNAIVPEIMKPDITAPGLEILAAYPPNLPP 502
Query: 508 ADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPSAIKSAIMTTAKPITSNDG 567
+ G K+ Y+ +SGTSM+CPH A +AAYVKSFH DWSP+AIKS+IMTT P+ ++
Sbjct: 503 SGKYGDKRSSKYTFLSGTSMACPHVAAIAAYVKSFHPDWSPAAIKSSIMTTTTPLKDSED 562
Query: 568 TIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGYDSKDIQLMSGDFNSSC-SS 627
E+ +GSG +NP +A+ PGLV++ SK+DYINLLC GYD+ I+ +SG+ NS+C SS
Sbjct: 563 K---EYAYGSGLVNPVKAINPGLVFDLSKDDYINLLCNIGYDTPKIRKISGE-NSTCPSS 622
Query: 628 NVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNVGDGSSSYKAEFLPFSKVYLTESTE 687
+D NYPA+A V P Q FV F R VTNVG +S+YK ++
Sbjct: 623 TPQKSVVKDFNYPALATHVQPDQPFVFNFTRTVTNVGFANSTYKVS---------VPTSS 682
Query: 688 KLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTALVWSDGIHKVRSPIVVEVKS 747
LN +V PQ +SF ++NEK+SF V V GG PDG+V S++L W+DG H VR PIVV+V
Sbjct: 683 DLNITVVPQVISFKSLNEKQSFVVKVVGGKFPDGSVPSSSLQWTDGTHNVRCPIVVDVSR 725
BLAST of Lag0010278 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 704.1 bits (1816), Expect = 1.2e-202
Identity = 374/689 (54.28%), Postives = 473/689 (68.65%), Query Frame = 0
Query: 54 STHLNLLESVVGTASETEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELK 113
S HL++L+ +VGT + HL+VRSYKRSFNGFAA L+ +E +KL M V+SVFPSK +
Sbjct: 13 SHHLSILQKLVGTIA-ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHE 72
Query: 114 PQTTRSWDFVGLRQKAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAG 173
TTRSWDFVG +KA+R V ESD+I+G D+GIWPESESF+D G GPPP++WKG+C G
Sbjct: 73 LTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKG 132
Query: 174 GANFTCNKKLIGARYYSA-SSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVP 233
G F CN KLIGAR+Y+ + SARD GHGTHTASTAAG+ V A F+GLA G ARG VP
Sbjct: 133 GLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVP 192
Query: 234 SARIAAYKVCESRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMT 293
SARIAAYKVC +RC++ D+LAAFDDAIADGVD+I+IS+ S ++AIGSFHAM
Sbjct: 193 SARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMM 252
Query: 294 KGILTVQSAGNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPP 353
+GI+T SAGN+GP ++ + +PW+ T+ A+ TDR +DRVVLGNGK +TG +VN+F
Sbjct: 253 RGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNL 312
Query: 354 NPNVLPLIYATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAI 413
N P++Y N SR C ++Q+ C GC+DSELVKGKIV C + G EA AGA G I
Sbjct: 313 NGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVI 372
Query: 414 VLNDKLVNVSLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFS 473
V N L + + V PFP +++ DY + +Y S + P A IL+++ I D AP +FS
Sbjct: 373 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 432
Query: 474 SRGPNAVMPEIMKPDVSAPGVEILAAFPPVLLPADL--PGFKQFVNYSIMSGTSMSCPHA 533
SRGP+ V+ ++KPDVSAPG+EILAAF PV P+ P K+ V YS+MSGTSM+CPH
Sbjct: 433 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 492
Query: 534 AGVAAYVKSFHRDWSPSAIKSAIMTTAKPITSNDGTIIGEFLHGSGQINPKEALKPGLVY 593
AGVAAYVKSFH DWSPSAIKSAIMTTA P+ EF +GSGQINP +A PGLVY
Sbjct: 493 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNP-EQEFAYGSGQINPTKASDPGLVY 552
Query: 594 ETSKEDYINLLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFV 653
E EDY+ +LC G+DS + SG N +CS +DLNYP M V F
Sbjct: 553 EVETEDYLKMLCAEGFDSTTLTTTSGQ-NVTCSERT---EVKDLNYPTMTTFVSSLDPFN 612
Query: 654 VKFVRIVTNVGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTV 713
V F R VTNVG +S+YKA +P +L S+EP+ L FG + EKKSF VT+
Sbjct: 613 VTFKRTVTNVGFPNSTYKASVVPLQ--------PELQISIEPEILRFGFLEEKKSFVVTI 672
Query: 714 AGGLIPDGTVFSTALVWSDGIHKVRSPIV 740
+G + DG+ S+++VWSDG H VRSPIV
Sbjct: 673 SGKELKDGSFVSSSVVWSDGSHSVRSPIV 687
BLAST of Lag0010278 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 666.8 bits (1719), Expect = 2.1e-191
Identity = 365/744 (49.06%), Postives = 509/744 (68.41%), Query Frame = 0
Query: 10 IQLMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASE 69
I L ++LL SS D + ++++ IVY+GS+ +R S H+++L+ + G S
Sbjct: 8 IFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITG-ESL 67
Query: 70 TEHLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ-- 129
E+ +VRSYK+SFNGFAA+LT+SE ++LA M V+SVFPS++LK QTT SW+F+GL++
Sbjct: 68 IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 127
Query: 130 KAKRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGAR 189
K KR ESD IIG D+GI+PES+SF+D G GPPP++WKGTCAGG NFTCN K+IGAR
Sbjct: 128 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 187
Query: 190 YYSASS----SARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE 249
Y+A S +ARD +GHGTHTAS AAG+ VA + F+GL G ARG VP+ARIA YKVC+
Sbjct: 188 DYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCD 247
Query: 250 SR-CHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAG 309
+ C +++AFDDAIADGVD+I+IS+ F+ D IAIG+FHAM G+LTV +AG
Sbjct: 248 NEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAG 307
Query: 310 NSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYA 369
N+GP ST+TS+APW+F++ A+ T+R+ + +VVLG+GK + G +VN++ N PL+Y
Sbjct: 308 NNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYG 367
Query: 370 TNAS-RTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 429
+A+ TC ++ C+P CLD +LVKGKIV C + G EA GA G+IV N + +
Sbjct: 368 KSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPE-PDR 427
Query: 430 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 489
+ + FP++ ++ DY + +Y S +NP A +LKS+ I + AP A+FSSRGP++++
Sbjct: 428 AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVS 487
Query: 490 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 549
+I+KPD++APGVEILAA+ P P + + V YS++SGTSM+CPH AGVAAYVK+FH
Sbjct: 488 DILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFH 547
Query: 550 RDWSPSAIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINL 609
WSPS I+SAIMTTA P+ S G + EF +GSG ++P +A+ PGLVYE +K D+IN
Sbjct: 548 PQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINF 607
Query: 610 LCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTNV 669
LCG Y S ++++SGD NS+C+ ++ R+LNYP M+A+V T+ F + F R VTNV
Sbjct: 608 LCGLNYTSDHLRIISGD-NSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 667
Query: 670 GDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTV 729
G S+Y A+ + F KL+ V P+ LS ++NEK+SF VTV+ I
Sbjct: 668 GMQKSTYNAKVVKF-------PGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 727
Query: 730 FSTALVWSDGIHKVRSPIVVEVKS 745
S L+WSDG H VRSPI+V S
Sbjct: 728 VSANLIWSDGTHNVRSPIIVYAMS 741
BLAST of Lag0010278 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 663.7 bits (1711), Expect = 1.8e-190
Identity = 367/741 (49.53%), Postives = 494/741 (66.67%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ + A + S + +++++ IVYMG++ +R S H ++L+ V G +S +
Sbjct: 9 LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
L VR+YKRSFNGFAA+LT+SE E LA+M+ V+SVFPSK L QTT SW+F+GL++ +
Sbjct: 69 RL-VRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 128
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN + ESD IIG D+GI+PES+SF+ G GPPP++WKG C GG NFTCN KLIGARYY
Sbjct: 129 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY 188
Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCES 251
+ SARD+TGHG+HTAS AAG+ V F+GL G RG VP+ARIA YKVC+
Sbjct: 189 TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDP 248
Query: 252 ---RCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
RC +LAAFDDAIAD VD+IT+S+G F+ D +AIG+FHAM KGILTV A
Sbjct: 249 GVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGA 308
Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
GN+GP T+ S APWLFT+ A+ +R+ + +VVLGNGKT+ G +VNSF N PL+Y
Sbjct: 309 GNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVY 368
Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
+AS C S + C PGCLDS+ VKGKIV C T EA GA +IV N +
Sbjct: 369 GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRN-PYEDA 428
Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
+ V FP++ ++ DY V +Y S +NP A +LKS+ I + AP A++SSRGPN ++
Sbjct: 429 ASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIH 488
Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
+I+KPD++APG EILAA+ P + P++ + V Y+++SGTSMSCPH AGVAAY+K+FH
Sbjct: 489 DILKPDITAPGSEILAAYSPYVPPSE--SDTRHVKYTVISGTSMSCPHVAGVAAYIKTFH 548
Query: 552 RDWSPSAIKSAIMTTAKPI--TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
WSPS I+SAIMTTA P+ +++ + EF +G+G ++P A+ PGLVYE +K D+I
Sbjct: 549 PLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHIT 608
Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
LCG Y K ++L+SGD +SSC+ T R+LNYP+M+AQV T+ F V F R VTN
Sbjct: 609 FLCGFNYTGKKLRLISGD-SSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTN 668
Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
VG +++YKA+ + KL V P LS ++ EKKSFTVTV+G
Sbjct: 669 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAEN 728
Query: 732 VFSTALVWSDGIHKVRSPIVV 741
+ S L+WSDG+H VRSPIVV
Sbjct: 729 LVSAQLIWSDGVHFVRSPIVV 734
BLAST of Lag0010278 vs. TAIR 10
Match:
AT3G46840.1 (Subtilase family protein )
HSP 1 Score: 650.2 bits (1676), Expect = 2.0e-186
Identity = 369/741 (49.80%), Postives = 492/741 (66.40%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ + A + S + ++++ IVYMG++ R S H ++L+ V G +S E
Sbjct: 10 LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESS-IE 69
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
+VR+YKRSFNGFAA+LT SE E LA+M+ V+SVFP+K+LK QTT SW+F+GL++ +
Sbjct: 70 DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN + ESD IIG D+GI+PES+SF+ G GPPP++WKG C GG NFT N KLIGARYY
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189
Query: 192 SAS-----SSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVCE- 251
+ SARD GHG+HTASTAAG+ V F+GL G ARG VP+ARIA YKVC+
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249
Query: 252 --SRCHEADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSA 311
C +LAAFDDAIAD VD+ITIS+GG S F+ D IAIG+FHAM KGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309
Query: 312 GNSGPAASTLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIY 371
GNSGP ST+ S APW+FT+ A+ T+R+ V +VVLGNGKTV G +VNSF N PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369
Query: 372 ATNASRTCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNV 431
+AS +C + + C PGCLDS+ VKGKIV C + EA GA +IV + + +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIVRSHR-TDV 429
Query: 432 SLVAPFPMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMP 491
+ + FP++ + DY V +Y S +NP A +LKS+ I + AP A++ SRGPN ++P
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489
Query: 492 EIMKPDVSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFH 551
+I+KPD++APG EI+AA+ P P+ + V YS+ +GTSMSCPH AGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549
Query: 552 RDWSPSAIKSAIMTTAKPITSNDGTI--IGEFLHGSGQINPKEALKPGLVYETSKEDYIN 611
WSPS I+SAIMTTA P+ ++ + EF +G+G ++P A+ PGLVYE +K D+I
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609
Query: 612 LLCGNGYDSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPTQAFVVKFVRIVTN 671
LCG Y +K+++L+SGD +SSC+ T R+LNYP+M AQV + F V F R VTN
Sbjct: 610 FLCGLNYTAKNLRLISGD-SSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTN 669
Query: 672 VGDGSSSYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGT 731
VG +++YKA+ + KL V P LS ++ EKKSFTVT +G
Sbjct: 670 VGRPNATYKAKVV----------GSKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAEN 729
Query: 732 VFSTALVWSDGIHKVRSPIVV 741
+ S L+WSDG+H VRSPIVV
Sbjct: 730 LVSAQLIWSDGVHFVRSPIVV 734
BLAST of Lag0010278 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 636.0 bits (1639), Expect = 3.9e-182
Identity = 364/735 (49.52%), Postives = 482/735 (65.58%), Query Frame = 0
Query: 12 LMAALLAAVCSSTFCDSGNIRERKLNIVYMGSIGNRGAAQLSSTHLNLLESVVGTASETE 71
L++ LL SS S ++++ IVYMGS+ +R +S H+N+L+ V G +S E
Sbjct: 10 LLSCLLVLFLSSV---SAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESS-IE 69
Query: 72 HLMVRSYKRSFNGFAAQLTDSEGEKLAAMNGVISVFPSKELKPQTTRSWDFVGLRQ--KA 131
+VRSYKRSFNGFAA+LT+SE E++A M GV+SVFP+K+L+ QTT SWDF+GL++ K
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKT 129
Query: 132 KRNVVSESDIIIGSFDTGIWPESESFNDVGLGPPPQRWKGTCAGGANFTCNKKLIGARYY 191
KRN ESD IIG D+GI PES+SF+D G GPPPQ+WKG C+GG NFTCN KLIGAR Y
Sbjct: 130 KRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 192 SASSSARDSTGHGTHTASTAAGSPVAGAEFFGLAAGVARGAVPSARIAAYKVC-ESRCHE 251
+ S RD GHGTHTASTAAG+ V A FFG+ G RG VP++R+AAYKVC + C
Sbjct: 190 T-SEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSS 249
Query: 252 ADVLAAFDDAIADGVDLITISVGGSGGSAFKFDAIAIGSFHAMTKGILTVQSAGNSGPAA 311
+L+AFDDAIADGVDLITIS+G S F+ D IAIG+FHAM KG+LTV SAGNSGP
Sbjct: 250 EALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKP 309
Query: 312 STLTSSAPWLFTIGATTTDRSIVDRVVLGNGKTVTGYTVNSFPPNPNVLPLIYATN-ASR 371
+++ APW+ T+ A+TT+R V +VVLGNGKT+ G +VN++ PL+Y + AS
Sbjct: 310 ISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASS 369
Query: 372 TCPRSQSERCQPGCLDSELVKGKIVQCMTYNGASEALNAGAAGAIVLNDKLVNVSLVAPF 431
C + C+ C+D VKGKI+ C G + GA G I K +V+ + P
Sbjct: 370 ACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLIYRTPK-PDVAFIHPL 429
Query: 432 PMTAVNVMDYGDVANYATSVQNPTARILKSQAIKDVSAPRAATFSSRGPNAVMPEIMKPD 491
P + D+ + +Y S +P A +LK++AI + ++P A+FSSRGPN + +I+KPD
Sbjct: 430 PAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPD 489
Query: 492 VSAPGVEILAAFPPVLLPADLPGFKQFVNYSIMSGTSMSCPHAAGVAAYVKSFHRDWSPS 551
++APGVEILAA+ P P+ + V YS++SGTSMSCPH AGVAAYVK+F+ WSPS
Sbjct: 490 ITAPGVEILAAYSPAGEPSQ--DDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPS 549
Query: 552 AIKSAIMTTAKPI-TSNDGTIIGEFLHGSGQINPKEALKPGLVYETSKEDYINLLCGNGY 611
I+SAIMTTA P+ + G EF +GSG ++P A PGLVYE K D+I LCG Y
Sbjct: 550 MIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNY 609
Query: 612 DSKDIQLMSGDFNSSCSSNVTNFPARDLNYPAMAAQVLPT-QAFVVKFVRIVTNVGDGSS 671
S+ ++++SG+ +CS P R+LNYP+M+A++ + F V F R +TNVG +S
Sbjct: 610 TSQVLKVISGE-TVTCSEAKKILP-RNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNS 669
Query: 672 SYKAEFLPFSKVYLTESTEKLNASVEPQELSFGAVNEKKSFTVTVAGGLIPDGTVFSTAL 731
+Y SKV + KL+ + P LSF VNEK+SFTVTV G + S L
Sbjct: 670 TYT------SKVVAGHGS-KLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANL 727
Query: 732 VWSDGIHKVRSPIVV 741
+WSDG H VRSPIVV
Sbjct: 730 IWSDGTHNVRSPIVV 727
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022958362.1 | 9.2e-258 | 63.78 | subtilisin-like protease SBT4.3 [Cucurbita moschata] | [more] |
KAG6605933.1 | 2.1e-257 | 63.38 | Subtilisin-like protease 4.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
XP_023533940.1 | 2.4e-253 | 62.45 | subtilisin-like protease SBT4.3 [Cucurbita pepo subsp. pepo] | [more] |
XP_022995032.1 | 2.6e-252 | 62.45 | subtilisin-like protease SBT4.3 [Cucurbita maxima] | [more] |
XP_022995031.1 | 1.9e-250 | 62.45 | subtilisin-like protease SBT4.3 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
Q9FIF8 | 2.0e-202 | 51.89 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
Q9FGU3 | 2.9e-190 | 49.06 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
Q9STF7 | 2.5e-189 | 49.53 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
F4JA91 | 2.8e-185 | 49.80 | Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... | [more] |
Q9FIG2 | 5.6e-181 | 49.52 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1H1M3 | 4.5e-258 | 63.78 | subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459607 PE... | [more] |
A0A6J1JXM0 | 1.3e-252 | 62.45 | subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490709 PE=3... | [more] |
A0A6J1K0V8 | 9.0e-251 | 62.45 | subtilisin-like protease SBT4.3 OS=Cucurbita maxima OX=3661 GN=LOC111490708 PE=3... | [more] |
A0A6J1H1W0 | 1.0e-246 | 61.10 | subtilisin-like protease SBT4.3 OS=Cucurbita moschata OX=3662 GN=LOC111459610 PE... | [more] |
A0A4P1R6C9 | 3.8e-209 | 54.22 | Uncharacterized protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_29596 PE=3 S... | [more] |