Lag0010212 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0010212
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionGolgin family A protein
Locationchr9: 45451451 .. 45455210 (+)
RNA-Seq ExpressionLag0010212
SyntenyLag0010212
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCATGGCCAGAGCAGAAGAAAACAGAGAAGAGATGTAAGATTAGAAAACGAGCGTGTTCATCGTCGTCTTCTTCTTCTTCGACTTTGGTCTTTAACTACAGATTCAACAAGTCCCCCACTTGGAAAATGAGCACCAAATCTCATTCGTCCAACCGCTCGCCGTCGTGCTCTCTGGACGGCGGCGGAAAAGGGAAAGAAGCGGCGGCGGCGGCGGCCTCTGTTTCCGTTTCAGTTTCGGCCCGGAAATCGACGGCCAACAACAATTCCCAGAAGCTGAAGAACAGTTCGGACGTGGTTGAAGATAAGAAAGAGCTGATCAAGACCCGGGAATTGGTTTCCCAGATCTCGCATTCGTGCTTATCAGATCCAGATCCGAGTTTGAAGAAGAACACAAATTCAGAGGTGAGTTCATGGTATGCATTGGGAATGGTGTTAATGAACTGGAAATTTGGCATTGATTTTGTAAATGGAATACGTGTTTGGATTTTGGTATCGAAATCTGAGAAAGTGGGGGTGAATTAAAAATGCAGAAAGTTGAAGGTGGCAGAGTACACAGAAGAAGAAGGTCGGCATCTTCTTTGAGGATTGGGATAGGGGAGGTGGGTGGTTCGAATTTTCATGGCAATCACTGTTTAATGGAGGTACGATTCATCTATTATTGCTTCCTTTCCACGAAATCATAATTCTTCGTTTTTTTTTTTCTTCCACTGTTTTCAATCCCATCAACCATCACTTGTTTTGGTTGCTGTCATTTTCCACTTGTACTTCTGCAGCAACCTTTTTTTCTTTCCTTTTCGTGATTCCCATAATCCCCTTTCAATTTTTAATTCGAATCTTCAATTTTTTTAAAAAAAATTTAGAAAGAATCCAAATGATTGATCATATATTTTTTTTTTAATATCAACAGTAAATACAAATTTTCGCTGTAATTATATGCTTTCCATACATAAGAAATAAACCATAACTTCTTTTTTAATGAAATGATTCGTCCTTACCATTTAAGCTTGGTCTTAATTTAGTCATATACGTTTTTATTTAGTGTTTCTATTGAATCTTTTGGTAAATTTACATTTCATCTCGAAATGGTAACTAACTCAAACTCATAACGACAGAGAGATAATTGGTGTGATTTGATTAAATCATAAACCAATTCGGACAATAAAAAAAATGAATTTTCAAGATGACAAACATATAGGGACCACATTGAAAGTTTAAAACCAAATTCAAACCTTTTTTCTTGTCTGTAATTCCAGTTTGAAAACTCGCTGTAATAAATATGAACAGAGGCAACGAACCATAAAAGCTGTTGCAGTATTCCAACTCCATTATTTATTTGTAATTTTAAAAGTATTGAGTAGTCAATCAGTCAATGAATATCCAATAATATTGAGTTGGAATGGAGGCAGATTGAAAATCCAAGTAGTCATGAGGGAAAAACGACACGTCGTAGTAAGACGAAATTGATGCTGAAAACGCGTTTGAAGGAAGTGAGCAATTGCCTGACGACATCGAAGGAGCTTCTAAGAGTTGTAAACCATGTGTGGGCTCATGAAGATCACGATGAACAGCGCCCATCTTCGACTTTGTCTCTGATTACGGCTCTGAAATCAGAGCTGGATCGAGCGAAAAGTCGAGTGGATCATTTGATCAAGGATCAGAGCTTTCATGGGGATGAAATCGAGCTGCTGATGAAGCGATTTGCAGAGGAGAAGGCGGCGTGGAAGTATAGGGAGAGAGCTAGAGTTCGAAGCGCCATTACTTCAATGGCGGATGAAGTTGAGGTGGAGAAGAAGCTTAGAAGACAGGCCGAGAGACTCAACAAGACCATTGCTAAAGAACTTGCTGATGCTAAAGTTTCACTTTCCAAAGCAATGAAAGATCTTCAAAGGTATTTTAACCACACCTAACCTTATAAAACGGATAAATTTCTTTCTTTTGTTATTTACGTTTTACAAATACTTTCAAAATTCAAGTTTAAGAAATAGTTAGGATTTGGTTTTTGTTTTTAAATTTTTACTATGAATTCCAATATTTCCTGAAATTGTGAGACAATTGTCCAAATTTAAAAATGAAAAACAAAAAGTCAAATGATTAATCAAACAGCTATTTCTCCAAATTTTATAATGATTTCTGATGTTGTTATCTATTTCTTAAAAGAAAAAACTTGGCTTCGGTAGGTAGGGATCTTCGTGCATACTACTATTAAAATGTTGACAACTAGGAGAGATAATTTTTTTTCTCATTTTTTCAAATCCCCTTCCTTAGTGGTCAAAATTTTAATGGATATGTAGGAAGATACCTACATAACGCCAGTCAGATTTTGTTCTTTCGTAAATTTCAAACATACCCTATTTTACTAATAAGTTTATAAGAATATAATATTAATAATAAAAAAATGTCCTTGTTTTCACTCTTGATAATTTTTTTAATTCTTAAAAACATATCATTCTTTTTAGAAATCGAGTATTCAATTTTTTTTTTTTAATTTTCAAAATTTGACTTAAAATTTGAGAACGTTCTTAAAAAGTAGATAAAGAAAAAAACTATATTGGTATAATTAGTGTAAGCTTAATTTTCAAAGTTAATAGATTATGATATATTTGATTAGTATTACGTCCCACCAGAATGGTTTTGTGAATTTTTTTTTTTTTTTTTTTGAAATATGGTTTTGTGAATTATATCATAAGATCGCATGAGTACTAATGTTTACTTGAGATAAAATGACATTGTCAACCATGTTCAAGTGAGGGTTTGGTTGTATCAAAAAGAAAAAAAAAAAAAAAAAAATTGAGGGTTTGGATTTTAGTAGATTTATAAACTTTATGTTGAATGCAAACTCGAAATTTAGAACTTGTTGTTATGAAACAAACTATAATAGACAACCAGCACAGTAAAAATTTTATTTATTTATTGCATTATTGTTGAGAAAAGGGAAAAGAGAGCAAAGGAGATATTCGAGCAGATCTGCGACGAATTAGCGAAAGGGATTGGAGAAGACAGAGCCCAGTTCGAGGAGCTGAAGAAGGAATCCGCCAAAGTGAGAGAAGAAGTTGAAAAAGAGAGAGAGATGCTTCAATTAGCCGACGTTTTGCGCGAGGAAAGAGTTCAGATGAAGCTCTCGGAGGCCAAATACCAGTTCGAAGAGAAAAACGCAGCCGTCGAGCGACTCAAGGACGAGCTCGAAGCCTATTTAATCACCCAGATCGGCAACGAACACGGCGAGGATTACTCCTGCAACAAATTCGAAAAAATCAAGGAGCTCGAAGCATACTTGAAGAAGATCAACTTCGGGTCGTGTCGAGAAGTCGAAACCAAAGAAGAAGACGATTGTTGTTCGGGGTCCGACGAGGACGAGAGCGATCTCCACTCCATAGAGCTCAACATGGACAACAACAGCAAGAGTTACAGATGGAGTTTCGTCCACAGCTCCTCCCAAAATCAAAACCCGTCCGAGAAAATCCAATGGGGAAGCATTTGCTTGAATCGGAAGTCCTCATCCAACGCTACTAAAAAGAGCCAGGAGAATTCAGAGAGATTTGACTGGGAGAGATTCACAGAGCTTTTTACGCAGAATCAGAAGGAGGAAAACGATAATACTAAATCTGTGAAGAAGAAGTGCCTTGGAGATATTCTGTTTCCAGGATCATCTGCGGAAGAACAGAACCAAGTTGTTGGTAAAACAGAGGATGATGGGACTGCCACGAATAATAATGTGGAAATGGACCAAGCTTGTTGTCCGGCGGCGACGAAGGGATGA

mRNA sequence

ATGTCATGGCCAGAGCAGAAGAAAACAGAGAAGAGATGTAAGATTAGAAAACGAGCGTGTTCATCGTCGTCTTCTTCTTCTTCGACTTTGGTCTTTAACTACAGATTCAACAAGTCCCCCACTTGGAAAATGAGCACCAAATCTCATTCGTCCAACCGCTCGCCGTCGTGCTCTCTGGACGGCGGCGGAAAAGGGAAAGAAGCGGCGGCGGCGGCGGCCTCTGTTTCCGTTTCAGTTTCGGCCCGGAAATCGACGGCCAACAACAATTCCCAGAAGCTGAAGAACAGTTCGGACGTGGTTGAAGATAAGAAAGAGCTGATCAAGACCCGGGAATTGGTTTCCCAGATCTCGCATTCGTGCTTATCAGATCCAGATCCGAGTTTGAAGAAGAACACAAATTCAGAGAAAGTTGAAGGTGGCAGAGTACACAGAAGAAGAAGGTCGGCATCTTCTTTGAGGATTGGGATAGGGGAGGTGGGTGGTTCGAATTTTCATGGCAATCACTGTTTAATGGAGATTGAAAATCCAAGTAGTCATGAGGGAAAAACGACACGTCGTAGTAAGACGAAATTGATGCTGAAAACGCGTTTGAAGGAAGTGAGCAATTGCCTGACGACATCGAAGGAGCTTCTAAGAGTTGTAAACCATGTGTGGGCTCATGAAGATCACGATGAACAGCGCCCATCTTCGACTTTGTCTCTGATTACGGCTCTGAAATCAGAGCTGGATCGAGCGAAAAGTCGAGTGGATCATTTGATCAAGGATCAGAGCTTTCATGGGGATGAAATCGAGCTGCTGATGAAGCGATTTGCAGAGGAGAAGGCGGCGTGGAAGTATAGGGAGAGAGCTAGAGTTCGAAGCGCCATTACTTCAATGGCGGATGAAGTTGAGGTGGAGAAGAAGCTTAGAAGACAGGCCGAGAGACTCAACAAGACCATTGCTAAAGAACTTGCTGATGCTAAAGTTTCACTTTCCAAAGCAATGAAAGATCTTCAAAGGGAAAAGAGAGCAAAGGAGATATTCGAGCAGATCTGCGACGAATTAGCGAAAGGGATTGGAGAAGACAGAGCCCAGTTCGAGGAGCTGAAGAAGGAATCCGCCAAAGTGAGAGAAGAAGTTGAAAAAGAGAGAGAGATGCTTCAATTAGCCGACGTTTTGCGCGAGGAAAGAGTTCAGATGAAGCTCTCGGAGGCCAAATACCAGTTCGAAGAGAAAAACGCAGCCGTCGAGCGACTCAAGGACGAGCTCGAAGCCTATTTAATCACCCAGATCGGCAACGAACACGGCGAGGATTACTCCTGCAACAAATTCGAAAAAATCAAGGAGCTCGAAGCATACTTGAAGAAGATCAACTTCGGGTCGTGTCGAGAAGTCGAAACCAAAGAAGAAGACGATTGTTGTTCGGGGTCCGACGAGGACGAGAGCGATCTCCACTCCATAGAGCTCAACATGGACAACAACAGCAAGAGTTACAGATGGAGTTTCGTCCACAGCTCCTCCCAAAATCAAAACCCGTCCGAGAAAATCCAATGGGGAAGCATTTGCTTGAATCGGAAGTCCTCATCCAACGCTACTAAAAAGAGCCAGGAGAATTCAGAGAGATTTGACTGGGAGAGATTCACAGAGCTTTTTACGCAGAATCAGAAGGAGGAAAACGATAATACTAAATCTGTGAAGAAGAAGTGCCTTGGAGATATTCTGTTTCCAGGATCATCTGCGGAAGAACAGAACCAAGTTGTTGGTAAAACAGAGGATGATGGGACTGCCACGAATAATAATGTGGAAATGGACCAAGCTTGTTGTCCGGCGGCGACGAAGGGATGA

Coding sequence (CDS)

ATGTCATGGCCAGAGCAGAAGAAAACAGAGAAGAGATGTAAGATTAGAAAACGAGCGTGTTCATCGTCGTCTTCTTCTTCTTCGACTTTGGTCTTTAACTACAGATTCAACAAGTCCCCCACTTGGAAAATGAGCACCAAATCTCATTCGTCCAACCGCTCGCCGTCGTGCTCTCTGGACGGCGGCGGAAAAGGGAAAGAAGCGGCGGCGGCGGCGGCCTCTGTTTCCGTTTCAGTTTCGGCCCGGAAATCGACGGCCAACAACAATTCCCAGAAGCTGAAGAACAGTTCGGACGTGGTTGAAGATAAGAAAGAGCTGATCAAGACCCGGGAATTGGTTTCCCAGATCTCGCATTCGTGCTTATCAGATCCAGATCCGAGTTTGAAGAAGAACACAAATTCAGAGAAAGTTGAAGGTGGCAGAGTACACAGAAGAAGAAGGTCGGCATCTTCTTTGAGGATTGGGATAGGGGAGGTGGGTGGTTCGAATTTTCATGGCAATCACTGTTTAATGGAGATTGAAAATCCAAGTAGTCATGAGGGAAAAACGACACGTCGTAGTAAGACGAAATTGATGCTGAAAACGCGTTTGAAGGAAGTGAGCAATTGCCTGACGACATCGAAGGAGCTTCTAAGAGTTGTAAACCATGTGTGGGCTCATGAAGATCACGATGAACAGCGCCCATCTTCGACTTTGTCTCTGATTACGGCTCTGAAATCAGAGCTGGATCGAGCGAAAAGTCGAGTGGATCATTTGATCAAGGATCAGAGCTTTCATGGGGATGAAATCGAGCTGCTGATGAAGCGATTTGCAGAGGAGAAGGCGGCGTGGAAGTATAGGGAGAGAGCTAGAGTTCGAAGCGCCATTACTTCAATGGCGGATGAAGTTGAGGTGGAGAAGAAGCTTAGAAGACAGGCCGAGAGACTCAACAAGACCATTGCTAAAGAACTTGCTGATGCTAAAGTTTCACTTTCCAAAGCAATGAAAGATCTTCAAAGGGAAAAGAGAGCAAAGGAGATATTCGAGCAGATCTGCGACGAATTAGCGAAAGGGATTGGAGAAGACAGAGCCCAGTTCGAGGAGCTGAAGAAGGAATCCGCCAAAGTGAGAGAAGAAGTTGAAAAAGAGAGAGAGATGCTTCAATTAGCCGACGTTTTGCGCGAGGAAAGAGTTCAGATGAAGCTCTCGGAGGCCAAATACCAGTTCGAAGAGAAAAACGCAGCCGTCGAGCGACTCAAGGACGAGCTCGAAGCCTATTTAATCACCCAGATCGGCAACGAACACGGCGAGGATTACTCCTGCAACAAATTCGAAAAAATCAAGGAGCTCGAAGCATACTTGAAGAAGATCAACTTCGGGTCGTGTCGAGAAGTCGAAACCAAAGAAGAAGACGATTGTTGTTCGGGGTCCGACGAGGACGAGAGCGATCTCCACTCCATAGAGCTCAACATGGACAACAACAGCAAGAGTTACAGATGGAGTTTCGTCCACAGCTCCTCCCAAAATCAAAACCCGTCCGAGAAAATCCAATGGGGAAGCATTTGCTTGAATCGGAAGTCCTCATCCAACGCTACTAAAAAGAGCCAGGAGAATTCAGAGAGATTTGACTGGGAGAGATTCACAGAGCTTTTTACGCAGAATCAGAAGGAGGAAAACGATAATACTAAATCTGTGAAGAAGAAGTGCCTTGGAGATATTCTGTTTCCAGGATCATCTGCGGAAGAACAGAACCAAGTTGTTGGTAAAACAGAGGATGATGGGACTGCCACGAATAATAATGTGGAAATGGACCAAGCTTGTTGTCCGGCGGCGACGAAGGGATGA

Protein sequence

MSWPEQKKTEKRCKIRKRACSSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSLDGGGKGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEAYLITQIGNEHGEDYSCNKFEKIKELEAYLKKINFGSCREVETKEEDDCCSGSDEDESDLHSIELNMDNNSKSYRWSFVHSSSQNQNPSEKIQWGSICLNRKSSSNATKKSQENSERFDWERFTELFTQNQKEENDNTKSVKKKCLGDILFPGSSAEEQNQVVGKTEDDGTATNNNVEMDQACCPAATKG
Homology
BLAST of Lag0010212 vs. NCBI nr
Match: KAG7035923.1 (hypothetical protein SDJN02_02723 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 738.4 bits (1905), Expect = 4.9e-209
Identity = 445/627 (70.97%), Postives = 504/627 (80.38%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRAC-SSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSL 60
           MSWPEQ KTE+ CKIRKR C SSSSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSCS+
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSSSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSCSI 60

Query: 61  DGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISH 120
            GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQISH
Sbjct: 61  AGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQISH 120

Query: 121 SCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSS 180
           SCLSDPDP    ++NS+KVEG RVHRRR SASS+R+G GE   +NFHGNHCL+EIENP S
Sbjct: 121 SCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSMRLGTGE---ANFHGNHCLIEIENP-S 180

Query: 181 HEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITAL 240
           ++G+T RR KTK MLKTRLKEV NCLTTSKEL+RV+NHV AHED+D+ RPSS   LITAL
Sbjct: 181 NQGRTVRR-KTKFMLKTRLKEVGNCLTTSKELIRVLNHVLAHEDNDQHRPSSISPLITAL 240

Query: 241 KSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEV 300
           KSE++RAK+RVDHLIKDQS HGDEIE++MKRF EEK AWK RERARVRS+I SMADE+E+
Sbjct: 241 KSEMERAKARVDHLIKDQSLHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEIEI 300

Query: 301 EKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQ 360
           EKKLRRQAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDRAQ
Sbjct: 301 EKKLRRQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDRAQ 360

Query: 361 FEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420
           FEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA
Sbjct: 361 FEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420

Query: 421 YLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCRE----VETKEEDDCCSGSDE 480
           +LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E     E  EE +C   S+E
Sbjct: 421 FLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHLEGDEKIEEQEC---SEE 480

Query: 481 DESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRKSS 540
           D+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK+S
Sbjct: 481 DDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDQINGRKSVSEKIQWGSICLNRKAS 540

Query: 541 SNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTKSVKKKCLGDILFPGSS 600
           + +       +KS E+SER +WERFTE+F +   N   E  NTKS   KCL DILFPG  
Sbjct: 541 NGSKNGEFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTKS--GKCLRDILFPG-- 593

BLAST of Lag0010212 vs. NCBI nr
Match: XP_022995046.1 (uncharacterized protein LOC111490718 isoform X1 [Cucurbita maxima] >XP_022995048.1 uncharacterized protein LOC111490718 isoform X1 [Cucurbita maxima])

HSP 1 Score: 738.0 bits (1904), Expect = 6.4e-209
Identity = 446/629 (70.91%), Postives = 505/629 (80.29%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRAC---SSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSC 60
           MSWPEQ KTE+ CKIRKR C   SSSSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSC
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSSSSSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSC 60

Query: 61  SLDGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQI 120
           S+ GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQI
Sbjct: 61  SVAGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQI 120

Query: 121 SHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENP 180
           SHSCLSDPDP    ++NS+KVEG RVHRRR SASSLRIG GE   +NFHGNHCL+EIENP
Sbjct: 121 SHSCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSLRIGTGE---ANFHGNHCLIEIENP 180

Query: 181 SSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLIT 240
            S++G+T RR KTK MLKTRLKEVSNCLTTSKEL+RV+NHV AHED+D+ RPSS   LIT
Sbjct: 181 -SNQGRTARR-KTKFMLKTRLKEVSNCLTTSKELVRVLNHVLAHEDNDQHRPSSISPLIT 240

Query: 241 ALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEV 300
           ALKSE++RAK+RVDHLIKDQSFHGDEIE++MKRF EEK AWK RERARVRS+I SMADE+
Sbjct: 241 ALKSEMERAKARVDHLIKDQSFHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEI 300

Query: 301 EVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDR 360
           E+EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDR
Sbjct: 301 EIEKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDR 360

Query: 361 AQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL 420
           AQFEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL
Sbjct: 361 AQFEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL 420

Query: 421 EAYLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCREVETK----EEDDCCSGS 480
           EA+LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E        EE +C   S
Sbjct: 421 EAFLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHPDGDGKIEEQEC---S 480

Query: 481 DEDESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRK 540
           +ED+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK
Sbjct: 481 EEDDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDQINGRKSVSEKIQWGSICLNRK 540

Query: 541 SSSNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTKSVKKKCLGDILFPG 600
           +S+ +       +KS E+SER +WERFTE+F +   N   E  NTKS   KCL DILFPG
Sbjct: 541 ASNGSKNGDFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTKS--GKCLRDILFPG 595

BLAST of Lag0010212 vs. NCBI nr
Match: XP_038900292.1 (uncharacterized protein At5g41620 isoform X1 [Benincasa hispida])

HSP 1 Score: 736.9 bits (1901), Expect = 1.4e-208
Identity = 442/622 (71.06%), Postives = 497/622 (79.90%), Query Frame = 0

Query: 2   SWPEQKKTEKRCKIRKRACSSSSSSSSTLVFNYRFNKSPTWKMSTKSHSS--------NR 61
           SW EQK  +KRCKIRKR C  SS SSSTLV  YRF K PTWKMSTKSHSS        NR
Sbjct: 3   SWAEQKTEKKRCKIRKRVC-LSSPSSSTLVRKYRFKKPPTWKMSTKSHSSKLSTGDLPNR 62

Query: 62  SPSCSLDGGGKGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELV 121
           SPSCSLDGGGKGKE        SVSVSARKS A NNSQKLKN+SDVVE+KKEL+KTRE+V
Sbjct: 63  SPSCSLDGGGKGKEG-------SVSVSARKS-AGNNSQKLKNNSDVVEEKKELMKTREMV 122

Query: 122 SQISHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGE-VGGSNFHGNHCLME 181
           SQISHSCLSDPD S+ KNT +EK E GRVHRRR SASSLRIGIGE VGGSNFHGN CLME
Sbjct: 123 SQISHSCLSDPDRSM-KNTKTEKDEVGRVHRRRGSASSLRIGIGEMVGGSNFHGNDCLME 182

Query: 182 IENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTL 241
           IEN   + GKTTRR KTK  +KTRLKEVSNCLTTSKELLRV++HV  HE+H    PSST 
Sbjct: 183 IEN--GNVGKTTRR-KTKSTIKTRLKEVSNCLTTSKELLRVLHHVLGHEEHP---PSSTS 242

Query: 242 SLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSM 301
           SLITALKSELDRAK+RVDHLIKDQ+FHGDEIE+L KRFAEEKAAWKYRERAR  SAI+SM
Sbjct: 243 SLITALKSELDRAKTRVDHLIKDQTFHGDEIEVLRKRFAEEKAAWKYRERARFGSAISSM 302

Query: 302 ADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGI 361
           A+EVEVEKKLRRQAERLNKTIAKELA+AKVS+SKAMK+++REKRAKEI EQIC+ELAKGI
Sbjct: 303 AEEVEVEKKLRRQAERLNKTIAKELAEAKVSVSKAMKEVEREKRAKEILEQICEELAKGI 362

Query: 362 GEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERL 421
           GEDRA+FEELKKESAKVREEVEKEREML LADVLREERVQMKLSEAKYQFEEKNAAVERL
Sbjct: 363 GEDRAEFEELKKESAKVREEVEKEREMLHLADVLREERVQMKLSEAKYQFEEKNAAVERL 422

Query: 422 KDELEAYLITQIGNE-----HGEDYSCNKFEKIKELEAYLKKINFGSCRE----VETKEE 481
           K +L+AYL+TQ GNE       ++YSCN+FEKIKELEAYLKKINFGSC++    V  +EE
Sbjct: 423 KHQLQAYLVTQFGNEEQNGGENQEYSCNEFEKIKELEAYLKKINFGSCQDSEKLVRKEEE 482

Query: 482 DDCCSGSDEDESDLHSIELNMDNNSKSYRWSFVHSSSQNQNP----------SEKIQWGS 541
           ++ CS  +E+ESDLHSIELNMDNN+KSYRWSFVH    + N           SEKIQWGS
Sbjct: 483 NEDCS-DEEEESDLHSIELNMDNNNKSYRWSFVHGEKADNNQIQSNNGRKSISEKIQWGS 542

Query: 542 ICLNRKSSSNATKKSQENSERFDWERFTELFTQNQKE-----ENDNTKSVKK-KCLGDIL 590
           ICLN  ++++     Q NS  FDW+ F+ELFTQ Q E     E D+   +K  KCL DIL
Sbjct: 543 ICLNTTNNTH-----QGNSNTFDWDTFSELFTQKQLEDLQELEADDDHQIKSVKCLRDIL 600

BLAST of Lag0010212 vs. NCBI nr
Match: XP_022957750.1 (uncharacterized protein LOC111459204 isoform X1 [Cucurbita moschata] >XP_022957751.1 uncharacterized protein LOC111459204 isoform X1 [Cucurbita moschata])

HSP 1 Score: 735.3 bits (1897), Expect = 4.1e-208
Identity = 443/627 (70.65%), Postives = 504/627 (80.38%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRACSS-SSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSL 60
           MSWPEQ KTE+ CKIRKR CSS SSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSCS+
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSLSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSCSI 60

Query: 61  DGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISH 120
            GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQISH
Sbjct: 61  AGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQISH 120

Query: 121 SCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSS 180
           SCLSDPDP    ++NS+KVEG RVHRRR SASS+R+G GE   +NFHG+HCL+EIENP S
Sbjct: 121 SCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSMRLGTGE---ANFHGDHCLIEIENP-S 180

Query: 181 HEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITAL 240
           ++GKT RR KTK MLKTRLKEV NCLTTSKEL+RV+NHV AHED+D+ RPSS   LITAL
Sbjct: 181 NQGKTARR-KTKFMLKTRLKEVGNCLTTSKELIRVLNHVLAHEDNDQHRPSSISPLITAL 240

Query: 241 KSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEV 300
           KSE++RAK+RVDHLIKDQS HGDEIE++MKRF EEK AWK RERARVRS+I SMADE+E+
Sbjct: 241 KSEMERAKARVDHLIKDQSLHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEIEI 300

Query: 301 EKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQ 360
           EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDRAQ
Sbjct: 301 EKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDRAQ 360

Query: 361 FEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420
           FEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA
Sbjct: 361 FEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420

Query: 421 YLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCRE----VETKEEDDCCSGSDE 480
           +LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E     E  EE +C   S+E
Sbjct: 421 FLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHLEGDEKIEEQEC---SEE 480

Query: 481 DESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRKSS 540
           D+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK+S
Sbjct: 481 DDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDEINGRKSVSEKIQWGSICLNRKAS 540

Query: 541 SNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTKSVKKKCLGDILFPGSS 600
           + +       +KS E+SER +WERFTE+F +   N   E  NTKS   KCL DILFPG  
Sbjct: 541 NGSKNGEFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTKS--GKCLRDILFPG-- 593

BLAST of Lag0010212 vs. NCBI nr
Match: XP_023534122.1 (uncharacterized protein LOC111795778 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023534127.1 uncharacterized protein LOC111795779 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 733.0 bits (1891), Expect = 2.1e-207
Identity = 442/626 (70.61%), Postives = 503/626 (80.35%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRAC-SSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSL 60
           MSWPEQ KTE+ CKIRKR C SSSSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSCS+
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSSSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSCSV 60

Query: 61  DGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISH 120
            GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQISH
Sbjct: 61  AGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQISH 120

Query: 121 SCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSS 180
           SCLSDPDP    ++NS+KVEG RVHRRR SASS+R+G GE   +NFHGNHCL+EIENP S
Sbjct: 121 SCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSMRLGTGE---ANFHGNHCLIEIENP-S 180

Query: 181 HEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITAL 240
           ++G+T RR KTK MLKTRLKEV NCLTTSKEL+RV+NHV AHED+D+ RPSS   LITAL
Sbjct: 181 NQGRTARR-KTKFMLKTRLKEVGNCLTTSKELIRVLNHVLAHEDNDQHRPSSISPLITAL 240

Query: 241 KSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEV 300
           K E++RAK+RVDHLIKDQSFHGDEIE++MKRF EEK AWK RERARVRS+I SMADE+E+
Sbjct: 241 KLEMERAKARVDHLIKDQSFHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEIEI 300

Query: 301 EKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQ 360
           EKKLRRQAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDRAQ
Sbjct: 301 EKKLRRQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDRAQ 360

Query: 361 FEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420
           FEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA
Sbjct: 361 FEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420

Query: 421 YLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCR---EVETKEEDDCCSGSDED 480
           +LITQ  +E+   EDYS     KIKELEAYLKKINFGS +   E + K E+  C  S+ED
Sbjct: 421 FLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHLEGDGKIEEQEC--SEED 480

Query: 481 ESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRKSSS 540
           +SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK+S+
Sbjct: 481 DSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDQINGRKSVSEKIQWGSICLNRKASN 540

Query: 541 NAT------KKSQENSERFDWERFTELFT---QNQKEENDNTKSVKKKCLGDILFPGSSA 600
            +       +KS E++ER +WERFTE+F     N   E  NTKS   KCL DILFPG   
Sbjct: 541 GSKNGEFVGRKSHESTERLEWERFTEVFENEGDNGSAEKKNTKS--GKCLRDILFPG--- 593

BLAST of Lag0010212 vs. ExPASy Swiss-Prot
Match: Q66GQ2 (Uncharacterized protein At5g41620 OS=Arabidopsis thaliana OX=3702 GN=At5g41620 PE=2 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.9e-18
Identity = 119/443 (26.86%), Postives = 218/443 (49.21%), Query Frame = 0

Query: 148 SASSLRIGIGEV----GGSNFHGNHCLMEIENP---SSHEGKTTRRSKT---KLMLKTR- 207
           SA SLR  IG++      S    NH L  +      SS E  T  ++ T    L  + R 
Sbjct: 126 SAGSLRRQIGQMLIKHHQSIDRNNHALQPVSPASYGSSLEVTTYNKAVTPSSSLEFRGRP 185

Query: 208 LKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQ 267
            +E    L TS ELL+V+N +W+     E++  S +SLI ALK+E+  ++ R+  L++ Q
Sbjct: 186 SREPHYNLKTSTELLKVLNRIWSL----EEQHVSNISLIKALKTEVAHSRVRIKELLRYQ 245

Query: 268 SFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKE 327
                E++ ++K+ AEEK   K +E  R+ SA+ S+   +E E+KLR+++E L++ +A+E
Sbjct: 246 QADRHELDSVVKQLAEEKLLSKNKEVERMSSAVQSVRKALEDERKLRKRSESLHRKMARE 305

Query: 328 LADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQFEELKKESAKV--REEVE 387
           L++ K SLS  +K+L+R  ++ ++ E +CDE AKGI     +   LKK++          
Sbjct: 306 LSEVKSSLSNCVKELERGSKSNKMMELLCDEFAKGIKSYEEEIHGLKKKNLDKDWAGRGG 365

Query: 388 KEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEAYLITQIGNEHGEDYSC 447
            ++ +L +A+   +ER+QM+L        +  + +++L+ E+E +L  +  NE   +   
Sbjct: 366 GDQLVLHIAESWLDERMQMRLEGGDTLNGKNRSVLDKLEVEIETFL-QEKRNEIPRNRR- 425

Query: 448 NKFEKI--KELEAYLKKINFGSCREVETKEEDDCCS--------GSDEDESDLHSIELNM 507
           N  E +    L A  + ++   C E     + +C          G +  + + H+ + ++
Sbjct: 426 NSLESVPFNTLSAPPRDVD---CEEDSGGSDSNCFELKKPAESYGDETKKPNQHNKDGSI 485

Query: 508 DNNSKSYRWSFVHSSSQNQNPSEKIQWGSICLNRKSSSNATKKSQENSERFDWERFTELF 567
           D   KS        SS   N  +++ W      +K ++ A +  +E       +   E  
Sbjct: 486 DEKPKS-------PSSFQVNFEDQMAWALSSNGKKKTTRAIEDEEEEE-----DVKPENS 545

BLAST of Lag0010212 vs. ExPASy Swiss-Prot
Match: F4I878 (Protein BRANCHLESS TRICHOME OS=Arabidopsis thaliana OX=3702 GN=BLT PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.1e-05
Identity = 43/130 (33.08%), Postives = 71/130 (54.62%), Query Frame = 0

Query: 281 ERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEI 340
           E  + +  I  +  E++ E+K RR+AE + K +A              KD++ E+ A+E 
Sbjct: 76  ELGKAQDEIKELKAELDYERKARRRAELMIKKLA--------------KDVEEERMAREA 135

Query: 341 FEQICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKY 400
            E     L K +  +       K E  +++ ++E+ER+M +LA+VLREERVQMKL +A+ 
Sbjct: 136 EEMQNKRLFKELSSE-------KSEMVRMKRDLEEERQMHRLAEVLREERVQMKLMDARL 184

Query: 401 QFEEKNAAVE 411
             EEK + +E
Sbjct: 196 FLEEKLSELE 184

BLAST of Lag0010212 vs. ExPASy TrEMBL
Match: A0A6J1K0X3 (uncharacterized protein LOC111490718 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490718 PE=4 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 3.1e-209
Identity = 446/629 (70.91%), Postives = 505/629 (80.29%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRAC---SSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSC 60
           MSWPEQ KTE+ CKIRKR C   SSSSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSC
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSSSSSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSC 60

Query: 61  SLDGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQI 120
           S+ GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQI
Sbjct: 61  SVAGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQI 120

Query: 121 SHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENP 180
           SHSCLSDPDP    ++NS+KVEG RVHRRR SASSLRIG GE   +NFHGNHCL+EIENP
Sbjct: 121 SHSCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSLRIGTGE---ANFHGNHCLIEIENP 180

Query: 181 SSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLIT 240
            S++G+T RR KTK MLKTRLKEVSNCLTTSKEL+RV+NHV AHED+D+ RPSS   LIT
Sbjct: 181 -SNQGRTARR-KTKFMLKTRLKEVSNCLTTSKELVRVLNHVLAHEDNDQHRPSSISPLIT 240

Query: 241 ALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEV 300
           ALKSE++RAK+RVDHLIKDQSFHGDEIE++MKRF EEK AWK RERARVRS+I SMADE+
Sbjct: 241 ALKSEMERAKARVDHLIKDQSFHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEI 300

Query: 301 EVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDR 360
           E+EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDR
Sbjct: 301 EIEKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDR 360

Query: 361 AQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL 420
           AQFEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL
Sbjct: 361 AQFEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDEL 420

Query: 421 EAYLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCREVETK----EEDDCCSGS 480
           EA+LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E        EE +C   S
Sbjct: 421 EAFLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHPDGDGKIEEQEC---S 480

Query: 481 DEDESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRK 540
           +ED+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK
Sbjct: 481 EEDDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDQINGRKSVSEKIQWGSICLNRK 540

Query: 541 SSSNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTKSVKKKCLGDILFPG 600
           +S+ +       +KS E+SER +WERFTE+F +   N   E  NTKS   KCL DILFPG
Sbjct: 541 ASNGSKNGDFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTKS--GKCLRDILFPG 595

BLAST of Lag0010212 vs. ExPASy TrEMBL
Match: A0A6J1H037 (uncharacterized protein LOC111459204 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459204 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 2.0e-208
Identity = 443/627 (70.65%), Postives = 504/627 (80.38%), Query Frame = 0

Query: 1   MSWPEQKKTEKRCKIRKRACSS-SSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSL 60
           MSWPEQ KTE+ CKIRKR CSS SSSSSSTLVF YRF  +PTWKMSTKSHSSNRSPSCS+
Sbjct: 1   MSWPEQ-KTEEICKIRKRRCSSLSSSSSSTLVFKYRFKNTPTWKMSTKSHSSNRSPSCSI 60

Query: 61  DGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISH 120
            GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++EDK+EL+KT++ VSQISH
Sbjct: 61  AGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIEDKQELMKTQDFVSQISH 120

Query: 121 SCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSS 180
           SCLSDPDP    ++NS+KVEG RVHRRR SASS+R+G GE   +NFHG+HCL+EIENP S
Sbjct: 121 SCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSMRLGTGE---ANFHGDHCLIEIENP-S 180

Query: 181 HEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITAL 240
           ++GKT RR KTK MLKTRLKEV NCLTTSKEL+RV+NHV AHED+D+ RPSS   LITAL
Sbjct: 181 NQGKTARR-KTKFMLKTRLKEVGNCLTTSKELIRVLNHVLAHEDNDQHRPSSISPLITAL 240

Query: 241 KSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEV 300
           KSE++RAK+RVDHLIKDQS HGDEIE++MKRF EEK AWK RERARVRS+I SMADE+E+
Sbjct: 241 KSEMERAKARVDHLIKDQSLHGDEIEIVMKRFTEEKTAWKNRERARVRSSIASMADEIEI 300

Query: 301 EKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQ 360
           EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFEQICDELAKGIGEDRAQ
Sbjct: 301 EKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFEQICDELAKGIGEDRAQ 360

Query: 361 FEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420
           FEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA
Sbjct: 361 FEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEA 420

Query: 421 YLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCRE----VETKEEDDCCSGSDE 480
           +LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E     E  EE +C   S+E
Sbjct: 421 FLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHLEGDEKIEEQEC---SEE 480

Query: 481 DESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNPSEKIQWGSICLNRKSS 540
           D+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ SEKIQWGSICLNRK+S
Sbjct: 481 DDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDEINGRKSVSEKIQWGSICLNRKAS 540

Query: 541 SNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTKSVKKKCLGDILFPGSS 600
           + +       +KS E+SER +WERFTE+F +   N   E  NTKS   KCL DILFPG  
Sbjct: 541 NGSKNGEFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTKS--GKCLRDILFPG-- 593

BLAST of Lag0010212 vs. ExPASy TrEMBL
Match: A0A6J1K315 (uncharacterized protein LOC111490718 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490718 PE=4 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 5.9e-192
Identity = 411/583 (70.50%), Postives = 468/583 (80.27%), Query Frame = 0

Query: 44  MSTKSHSSNRSPSCSLDGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVED 103
           MSTKSHSSNRSPSCS+ GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++ED
Sbjct: 1   MSTKSHSSNRSPSCSVAGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIED 60

Query: 104 KKELIKTRELVSQISHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGS 163
           K+EL+KT++ VSQISHSCLSDPDP    ++NS+KVEG RVHRRR SASSLRIG GE   +
Sbjct: 61  KQELMKTQDFVSQISHSCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSLRIGTGE---A 120

Query: 164 NFHGNHCLMEIENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHED 223
           NFHGNHCL+EIENP S++G+T RR KTK MLKTRLKEVSNCLTTSKEL+RV+NHV AHED
Sbjct: 121 NFHGNHCLIEIENP-SNQGRTARR-KTKFMLKTRLKEVSNCLTTSKELVRVLNHVLAHED 180

Query: 224 HDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRER 283
           +D+ RPSS   LITALKSE++RAK+RVDHLIKDQSFHGDEIE++MKRF EEK AWK RER
Sbjct: 181 NDQHRPSSISPLITALKSEMERAKARVDHLIKDQSFHGDEIEIVMKRFTEEKTAWKNRER 240

Query: 284 ARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFE 343
           ARVRS+I SMADE+E+EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFE
Sbjct: 241 ARVRSSIASMADEIEIEKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFE 300

Query: 344 QICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQF 403
           QICDELAKGIGEDRAQFEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQF
Sbjct: 301 QICDELAKGIGEDRAQFEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQF 360

Query: 404 EEKNAAVERLKDELEAYLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCREVET 463
           EEKNAAVERLKDELEA+LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E   
Sbjct: 361 EEKNAAVERLKDELEAFLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHPD 420

Query: 464 K----EEDDCCSGSDEDESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNP 523
                EE +C   S+ED+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ 
Sbjct: 421 GDGKIEEQEC---SEEDDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDQINGRKSV 480

Query: 524 SEKIQWGSICLNRKSSSNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTK 583
           SEKIQWGSICLNRK+S+ +       +KS E+SER +WERFTE+F +   N   E  NTK
Sbjct: 481 SEKIQWGSICLNRKASNGSKNGDFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTK 540

Query: 584 SVKKKCLGDILFPGSSAEEQNQVVGKTEDDGTATNNNVEMDQA 600
           S   KCL DILFPG         V   +D G A   NVEMD+A
Sbjct: 541 S--GKCLRDILFPG--------FVEPNDDVGIA--GNVEMDEA 550

BLAST of Lag0010212 vs. ExPASy TrEMBL
Match: A0A6J1H001 (uncharacterized protein LOC111459204 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459204 PE=4 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 6.5e-191
Identity = 408/583 (69.98%), Postives = 467/583 (80.10%), Query Frame = 0

Query: 44  MSTKSHSSNRSPSCSLDGGG-KGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVED 103
           MSTKSHSSNRSPSCS+ GGG KGKE   A+ SVSVSVSAR     N+SQKLKN+ D++ED
Sbjct: 1   MSTKSHSSNRSPSCSIAGGGSKGKE---ASVSVSVSVSAR-----NHSQKLKNNMDIIED 60

Query: 104 KKELIKTRELVSQISHSCLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGGS 163
           K+EL+KT++ VSQISHSCLSDPDP    ++NS+KVEG RVHRRR SASS+R+G GE   +
Sbjct: 61  KQELMKTQDFVSQISHSCLSDPDPCF-NDSNSKKVEGDRVHRRRTSASSMRLGTGE---A 120

Query: 164 NFHGNHCLMEIENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHED 223
           NFHG+HCL+EIENP S++GKT RR KTK MLKTRLKEV NCLTTSKEL+RV+NHV AHED
Sbjct: 121 NFHGDHCLIEIENP-SNQGKTARR-KTKFMLKTRLKEVGNCLTTSKELIRVLNHVLAHED 180

Query: 224 HDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRER 283
           +D+ RPSS   LITALKSE++RAK+RVDHLIKDQS HGDEIE++MKRF EEK AWK RER
Sbjct: 181 NDQHRPSSISPLITALKSEMERAKARVDHLIKDQSLHGDEIEIVMKRFTEEKTAWKNRER 240

Query: 284 ARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFE 343
           ARVRS+I SMADE+E+EKKLR+QAERLNKTIAKELA+AK+SLSKAMKDLQRE+RAKEIFE
Sbjct: 241 ARVRSSIASMADEIEIEKKLRKQAERLNKTIAKELAEAKISLSKAMKDLQRERRAKEIFE 300

Query: 344 QICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQF 403
           QICDELAKGIGEDRAQFEE KKESAKVREE+E+EREMLQLADVLREERVQMKLSEAKYQF
Sbjct: 301 QICDELAKGIGEDRAQFEEFKKESAKVREEIEQEREMLQLADVLREERVQMKLSEAKYQF 360

Query: 404 EEKNAAVERLKDELEAYLITQIGNEH--GEDYSCNKFEKIKELEAYLKKINFGSCRE--- 463
           EEKNAAVERLKDELEA+LITQ  +E+   EDYS     KIKELEAYLKKINFGS +E   
Sbjct: 361 EEKNAAVERLKDELEAFLITQFRHENREEEDYS----GKIKELEAYLKKINFGSVQEHLE 420

Query: 464 -VETKEEDDCCSGSDEDESDLHSIELNMDNNSKSYRWSFVHSSSQ-----------NQNP 523
             E  EE +C   S+ED+SDLHSIELNMDNN+KSYRWSFVH  S+            ++ 
Sbjct: 421 GDEKIEEQEC---SEEDDSDLHSIELNMDNNNKSYRWSFVHGGSKRNSFEKDEINGRKSV 480

Query: 524 SEKIQWGSICLNRKSSSNAT------KKSQENSERFDWERFTELFTQ---NQKEENDNTK 583
           SEKIQWGSICLNRK+S+ +       +KS E+SER +WERFTE+F +   N   E  NTK
Sbjct: 481 SEKIQWGSICLNRKASNGSKNGEFVGRKSHESSERLEWERFTEVFEKEGDNGSAEKKNTK 540

Query: 584 SVKKKCLGDILFPGSSAEEQNQVVGKTEDDGTATNNNVEMDQA 600
           S   KCL DILFPG         V   +D G A   NVEMD+A
Sbjct: 541 S--GKCLRDILFPG--------FVEPNDDVGIA--GNVEMDEA 550

BLAST of Lag0010212 vs. ExPASy TrEMBL
Match: A0A0A0KEV9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G425750 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 2.7e-173
Identity = 399/639 (62.44%), Postives = 465/639 (72.77%), Query Frame = 0

Query: 2   SWPEQKKTEKRCKIRKRACSSSSSSSSTLVFNYRFNKSPTWKMST--KSHSS-------- 61
           SW EQK  +K+CKIRKR C  SS SSST V  YRF K PTWKMST  KSHSS        
Sbjct: 3   SWAEQKTEKKKCKIRKRVC-LSSPSSSTFVRKYRFKKPPTWKMSTKSKSHSSKLSTTDDI 62

Query: 62  -NRSPSCSLDGGGKGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTR 121
            NRSPSCS++ G + +E      SV              S+ LK +S+VVED     K+R
Sbjct: 63  VNRSPSCSVNKGKEEEEGGGGGGSV--------------SRILKKNSEVVED-----KSR 122

Query: 122 ELVSQISHSCLSDPDPSLKKNTNSEKVEGG---RVHRRRRSASS---LRIGIGE-VGGSN 181
           ELVS+IS + LSDPD S+K    +EK E G   RVHRRRRSA++   LRIG GE VGGSN
Sbjct: 123 ELVSEISETNLSDPDRSVKNTKTTEKDEIGTMKRVHRRRRSAATEPCLRIGNGEMVGGSN 182

Query: 182 FHGNHCL-MEIENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAHED 241
           FHGN CL MEIEN   +  KTTRR KTK  +KTRLKEVSNCLTTSKELLRV++H+  HED
Sbjct: 183 FHGNDCLTMEIEN--GNVEKTTRR-KTKTTVKTRLKEVSNCLTTSKELLRVLHHILLHED 242

Query: 242 HDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRER 301
           H    PSST SLI+ALKSELDRAK+RVDHLIKDQ+F+ DEIE+L +R AEEKAAWKYRER
Sbjct: 243 H---LPSSTSSLISALKSELDRAKTRVDHLIKDQTFNVDEIEVLKRRLAEEKAAWKYRER 302

Query: 302 ARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFE 361
           AR  SAI+SMA+E+E+EKKLRRQAERLNK+IAKELA+AKVS+SKAMK+++REKRAKEI E
Sbjct: 303 ARFGSAISSMAEEMEIEKKLRRQAERLNKSIAKELAEAKVSVSKAMKEVEREKRAKEILE 362

Query: 362 QICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQF 421
           QIC+ELAKGIGEDRA+FEELKKESAKVREEVEKEREML LADVLREERVQMKLSEAKYQF
Sbjct: 363 QICEELAKGIGEDRAEFEELKKESAKVREEVEKEREMLHLADVLREERVQMKLSEAKYQF 422

Query: 422 EEKNAAVERLKDELEAYLITQIGNE-----HGEDYSCNKFEKIKELEAYLKKINFGSCRE 481
           EEKNAAVERLK +L+ Y +  IGNE        +YSCN+FEKIKELEAYLKKINFGSC++
Sbjct: 423 EEKNAAVERLKHQLQGYFV--IGNEEQNAGENREYSCNEFEKIKELEAYLKKINFGSCQD 482

Query: 482 VE---TKEEDDCCSG------SDEDESDLHSIELNMDNNSKSYRWSFVHSSSQNQ----- 541
            E    KEE+  CS        +E+ESD+HSIELNMDNN+KSYRWSFV  +  NQ     
Sbjct: 483 TEKMGKKEENGDCSDEEEEEEEEEEESDMHSIELNMDNNNKSYRWSFVEKADNNQIQINN 542

Query: 542 ---NPSEKIQWGSICLNRKSSSNATKKSQENSERFDWERFTELFTQNQKEE--------- 590
              + SEKIQWGSICLN  +S+N T   Q+NS  FDW+ F+ELFT+   EE         
Sbjct: 543 GRKSVSEKIQWGSICLN--TSNNNT--HQQNSNSFDWDTFSELFTRKNLEELHDQLDDDD 602

BLAST of Lag0010212 vs. TAIR 10
Match: AT3G11590.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G22310.1); Has 22320 Blast hits to 15179 proteins in 1213 species: Archae - 372; Bacteria - 2307; Metazoa - 10906; Fungi - 1700; Plants - 1146; Viruses - 65; Other Eukaryotes - 5824 (source: NCBI BLink). )

HSP 1 Score: 282.0 bits (720), Expect = 1.2e-75
Identity = 235/597 (39.36%), Postives = 328/597 (54.94%), Query Frame = 0

Query: 14  KIRKRACSS-SSSSSSTLVFNYRFNKS-------------PTWKMSTKSHSSNRS----- 73
           KIRKR CSS +SS+SS L   YRF ++             PTW++  +S S   S     
Sbjct: 16  KIRKRGCSSPTSSTSSILREGYRFKRAIVVGKRGGSTTPVPTWRLMGRSPSPRASGALHA 75

Query: 74  -PSCSLDGGGKGKEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIK--TRE 133
             S S   G K  + +A A      VSARK  A         S  VVE+   +I+   +E
Sbjct: 76  AASPSSHCGSKTGKVSAPA-----PVSARKLAATLWEMNEMPSPRVVEEAAPMIRKSRKE 135

Query: 134 LVSQIS------HS-----CLSDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVG 193
            ++ +       HS      LSDP  S           G R  R   +   LR+G   VG
Sbjct: 136 RIAPLPPPRSSVHSGSLPPHLSDPSHSPVSERMERSGTGSRQRRASSTVQKLRLGDCNVG 195

Query: 194 GSNFHGNHCLMEIENPSSHEGKTTRRSKTKLMLKTRLKEVSNCLTTSKELLRVVNHVWAH 253
             +   +   M+IE  S  E  T     + + +KTRLK+ SN LTTSKELL+++N +W  
Sbjct: 196 ARDPINSGSFMDIETRSRVETPT----GSTVGVKTRLKDCSNALTTSKELLKIINRMWGQ 255

Query: 254 EDHDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYR 313
           +D    RPSS++SL++AL SEL+RA+ +V+ LI +     ++I  LMKRFAEEKA WK  
Sbjct: 256 DD----RPSSSMSLVSALHSELERARLQVNQLIHEHKPENNDISYLMKRFAEEKAVWKSN 315

Query: 314 ERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEI 373
           E+  V +AI S+A E+EVE+KLRR+ E LNK + KELA+ K +L KA+K+++ EKRA+ +
Sbjct: 316 EQEVVEAAIESVAGELEVERKLRRRFESLNKKLGKELAETKSALMKAVKEIENEKRARVM 375

Query: 374 FEQICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKY 433
            E++CDELA+ I ED+A+ EELK+ES KV+EEVEKEREMLQLAD LREERVQMKLSEAK+
Sbjct: 376 VEKVCDELARDISEDKAEVEELKRESFKVKEEVEKEREMLQLADALREERVQMKLSEAKH 435

Query: 434 QFEEKNAAVERLKDELEAYLITQIGNEHGEDYSCNKFEKIKELEAYLKKINFGSCREVET 493
           Q EEKNAAV++L+++L+ YL  +   E   +    +    +  +     I+FGS    + 
Sbjct: 436 QLEEKNAAVDKLRNQLQTYLKAKRCKEKTREPPQTQLHNEEAGDYLNHHISFGSYNIEDG 495

Query: 494 KEEDDCCSGSDEDESDLHSIELNMDNNSKSYRWSFVHSSSQNQNPSEKIQWGSICLNRKS 553
           + E+    GS   ESDLHSIELN+DN  KSY+W +   +   ++   K    S+ L R  
Sbjct: 496 EVENGNEEGS--GESDLHSIELNIDN--KSYKWPYGEENRGRKSTPRK----SLSLQRSI 555

Query: 554 SS------NATKKSQENSERFDWERFTELFTQNQKEENDNTKSVKKKCLGDILFPGS 572
           S        + K  +      DW R  ++  +   +E    K  K       +  GS
Sbjct: 556 SDCVDWVVQSEKLQKSGDGGLDWGRSIDVEPKGYIDETQAYKPNKASSKDHHILSGS 591

BLAST of Lag0010212 vs. TAIR 10
Match: AT1G50660.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20350.1); Has 21445 Blast hits to 15134 proteins in 1325 species: Archae - 461; Bacteria - 2309; Metazoa - 11052; Fungi - 1737; Plants - 1035; Viruses - 42; Other Eukaryotes - 4809 (source: NCBI BLink). )

HSP 1 Score: 153.3 bits (386), Expect = 6.3e-37
Identity = 117/402 (29.10%), Postives = 220/402 (54.73%), Query Frame = 0

Query: 133 NSEKVEGGRVHRRRRSASSLRIGIGEVGGSNFHGNHCLMEIENPSSHEGKTTRRSKTKLM 192
           +S+K  GG+ ++ R++ S++         +  +G  C +E   P  H             
Sbjct: 161 HSDKPSGGQSNKIRQNPSTI--------ATTKNGFLCKLEPSMPFPHSA----------- 220

Query: 193 LKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITALKSELDRAKSRVDHL 252
           ++   K    CL T +E    V+ ++++    +Q+ ++ +SL+++L++EL+ A +R++ L
Sbjct: 221 MEGATKWDPVCLDTMEE----VHQIYSNMKRIDQQVNA-VSLVSSLEAELEEAHARIEDL 280

Query: 253 IKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEVEKKLRRQAERLNKT 312
             ++  H  ++E  +++ +EE+AAW+ RE  +VR+ I  M  ++  EKK R++ E +N  
Sbjct: 281 ESEKRSHKKKLEQFLRKVSEERAAWRSREHEKVRAIIDDMKTDMNREKKTRQRLEIVNHK 340

Query: 313 IAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQFEELKKESAKVREE 372
           +  ELAD+K+++ + M+D ++E++A+E+ E++CDELAK IGED+A+ E LK+ES  +REE
Sbjct: 341 LVNELADSKLAVKRYMQDYEKERKARELIEEVCDELAKEIGEDKAEIEALKRESMSLREE 400

Query: 373 VEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEAYL-----ITQIGNE 432
           V+ ER MLQ+A+V REERVQMKL +AK   EE+ + + +L  +LE++L     +T +   
Sbjct: 401 VDDERRMLQMAEVWREERVQMKLIDAKVALEERYSQMNKLVGDLESFLRSRDIVTDVKEV 460

Query: 433 H-----GEDYSCNKFEKIKE----------LEAYLKKINFGSCREVETKEEDDCCSGSDE 492
                  E  +    ++IKE          + A  +++N G   + E   E         
Sbjct: 461 REAELLRETAASVNIQEIKEFTYVPANPDDIYAVFEEMNLGEAHDREM--EKSVAYSPIS 520

Query: 493 DESDLHSIELNMDNNSKSYRWSFVHSSSQNQNPSEKIQWGSI 515
            +S +H++ L+ +  +K  R S  ++        +   W ++
Sbjct: 521 HDSKVHTVSLDANMMNKKGRHSDAYTHQNGDIEEDDSGWETV 536

BLAST of Lag0010212 vs. TAIR 10
Match: AT5G22310.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G11590.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 150.6 bits (379), Expect = 4.1e-36
Identity = 174/507 (34.32%), Postives = 255/507 (50.30%), Query Frame = 0

Query: 5   EQKKTEKRCKIRKRACSSSSSSSSTLVFNYRFNKSPTWKMSTKSHSSNRSPSCSLDGGGK 64
           EQ+K  K CKIRKR    SSSSSS+L    RF ++          +  R+   + D GG 
Sbjct: 2   EQRK--KGCKIRKR--GGSSSSSSSLARRNRFKRA--------IFAGKRA---AQDDGGS 61

Query: 65  G---KEAAAAAASVSVSVSARKSTANNNSQKLKNSSDVVEDKKELIKTRELVSQISHSCL 124
           G   K   AA   V +S S      +++           + +K  +  R+L + +     
Sbjct: 62  GTPVKSITAAKTPVLLSFSPENLPIDHH-----------QLQKSCVSARKLAATL-WEIN 121

Query: 125 SDPDPSLKKNTNSEKVEGGRVHRRRRSASSLRIGIGEVGG------SNFHGNHCLMEIEN 184
            D DP +  + +  + +    +R ++S     I             S+   + C   I  
Sbjct: 122 DDADPPVNSDKDCLRSKKPSRYRAKKSTEFSSIDFPPRSSDPISRLSSERIDLCDDMIRR 181

Query: 185 PSSHEGKTTRRSKTKL---MLKTRLKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTL 244
            S++  K        +    +KTR K VS+ LTTSKEL++V+  +   E  D+ + +S  
Sbjct: 182 RSTNPQKLNPIEYKIIGANSVKTRFKNVSDGLTTSKELVKVLKRI--GELGDDHKTASN- 241

Query: 245 SLITALKSELDRAKSRVDHLIKDQSFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSM 304
            LI+AL  ELDRA+S + HL+ +     DE E       EEK           R  I S+
Sbjct: 242 RLISALLCELDRARSSLKHLMSEL----DEEE-------EEK-----------RRLIESL 301

Query: 305 ADEVEVEKKLRRQAERLNKTIAKELADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGI 364
            +E  VE+KLRR+ E++N+ + +EL +AK +  K  ++++REKRAK++ E++CDEL KGI
Sbjct: 302 QEEAMVERKLRRRTEKMNRRLGRELTEAKETERKMKEEMKREKRAKDVLEEVCDELTKGI 361

Query: 365 GEDRAQFEELKKESAKVREEVEKEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERL 424
           G+D              ++E+EKEREM+ +ADVLREERVQMKL+EAK++FE+K AAVERL
Sbjct: 362 GDD--------------KKEMEKEREMMHIADVLREERVQMKLTEAKFEFEDKYAAVERL 412

Query: 425 KDELEAYLITQIGNEHGEDYSCNKFEKIKELEAYLKKINFGSCREVETKEEDDCCSGSDE 484
           K EL   L      E G+  S        E+   L+ I+                SGSD+
Sbjct: 422 KKELRRVL----DGEEGKGSS--------EIRRILEVID---------------GSGSDD 412

Query: 485 D-ESDLHSIELNMDNNSKSYRWSFVHS 499
           D ESDL SIELNM++ SK   W +V S
Sbjct: 482 DEESDLKSIELNMESGSK---WGYVDS 412

BLAST of Lag0010212 vs. TAIR 10
Match: AT3G20350.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: cotyledon; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G50660.1); Has 15095 Blast hits to 11224 proteins in 1051 species: Archae - 223; Bacteria - 1586; Metazoa - 7000; Fungi - 1255; Plants - 746; Viruses - 40; Other Eukaryotes - 4245 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 1.3e-34
Identity = 85/219 (38.81%), Postives = 145/219 (66.21%), Query Frame = 0

Query: 203 CLTTSKELLRVVNHV-WAHEDHDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQSFHGD 262
           CL T  ++ ++  +V W ++  ++      +SL ++++ +L  A++ +  L  ++     
Sbjct: 189 CLDTRDDVHQIYTNVKWNNQQVND------VSLASSIELKLQEARACIKDLESEKRSQKK 248

Query: 263 EIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKELADAK 322
           ++E  +K+ +EE+AAW+ RE  +VR+ I  M  ++  EKK R++ E +N  +  ELAD+K
Sbjct: 249 KLEQFLKKVSEERAAWRSREHEKVRAIIDDMKADMNQEKKTRQRLEIVNSKLVNELADSK 308

Query: 323 VSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQFEELKKESAKVREEVEKEREMLQ 382
           +++ + M D Q+E++A+E+ E++CDELAK I ED+A+ E LK ES  +REEV+ ER MLQ
Sbjct: 309 LAVKRYMHDYQQERKARELIEEVCDELAKEIEEDKAEIEALKSESMNLREEVDDERRMLQ 368

Query: 383 LADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEAYL 421
           +A+V REERVQMKL +AK   EEK + + +L  ++EA+L
Sbjct: 369 MAEVWREERVQMKLIDAKVTLEEKYSQMNKLVGDMEAFL 401

BLAST of Lag0010212 vs. TAIR 10
Match: AT5G41620.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast, plasma membrane; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: 6 growth stages; BEST Arabidopsis thaliana protein match is: intracellular protein transport protein USO1-related (TAIR:AT1G64180.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 95.1 bits (235), Expect = 2.0e-19
Identity = 119/443 (26.86%), Postives = 218/443 (49.21%), Query Frame = 0

Query: 148 SASSLRIGIGEV----GGSNFHGNHCLMEIENP---SSHEGKTTRRSKT---KLMLKTR- 207
           SA SLR  IG++      S    NH L  +      SS E  T  ++ T    L  + R 
Sbjct: 126 SAGSLRRQIGQMLIKHHQSIDRNNHALQPVSPASYGSSLEVTTYNKAVTPSSSLEFRGRP 185

Query: 208 LKEVSNCLTTSKELLRVVNHVWAHEDHDEQRPSSTLSLITALKSELDRAKSRVDHLIKDQ 267
            +E    L TS ELL+V+N +W+     E++  S +SLI ALK+E+  ++ R+  L++ Q
Sbjct: 186 SREPHYNLKTSTELLKVLNRIWSL----EEQHVSNISLIKALKTEVAHSRVRIKELLRYQ 245

Query: 268 SFHGDEIELLMKRFAEEKAAWKYRERARVRSAITSMADEVEVEKKLRRQAERLNKTIAKE 327
                E++ ++K+ AEEK   K +E  R+ SA+ S+   +E E+KLR+++E L++ +A+E
Sbjct: 246 QADRHELDSVVKQLAEEKLLSKNKEVERMSSAVQSVRKALEDERKLRKRSESLHRKMARE 305

Query: 328 LADAKVSLSKAMKDLQREKRAKEIFEQICDELAKGIGEDRAQFEELKKESAKV--REEVE 387
           L++ K SLS  +K+L+R  ++ ++ E +CDE AKGI     +   LKK++          
Sbjct: 306 LSEVKSSLSNCVKELERGSKSNKMMELLCDEFAKGIKSYEEEIHGLKKKNLDKDWAGRGG 365

Query: 388 KEREMLQLADVLREERVQMKLSEAKYQFEEKNAAVERLKDELEAYLITQIGNEHGEDYSC 447
            ++ +L +A+   +ER+QM+L        +  + +++L+ E+E +L  +  NE   +   
Sbjct: 366 GDQLVLHIAESWLDERMQMRLEGGDTLNGKNRSVLDKLEVEIETFL-QEKRNEIPRNRR- 425

Query: 448 NKFEKI--KELEAYLKKINFGSCREVETKEEDDCCS--------GSDEDESDLHSIELNM 507
           N  E +    L A  + ++   C E     + +C          G +  + + H+ + ++
Sbjct: 426 NSLESVPFNTLSAPPRDVD---CEEDSGGSDSNCFELKKPAESYGDETKKPNQHNKDGSI 485

Query: 508 DNNSKSYRWSFVHSSSQNQNPSEKIQWGSICLNRKSSSNATKKSQENSERFDWERFTELF 567
           D   KS        SS   N  +++ W      +K ++ A +  +E       +   E  
Sbjct: 486 DEKPKS-------PSSFQVNFEDQMAWALSSNGKKKTTRAIEDEEEEE-----DVKPENS 545

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035923.14.9e-20970.97hypothetical protein SDJN02_02723 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022995046.16.4e-20970.91uncharacterized protein LOC111490718 isoform X1 [Cucurbita maxima] >XP_022995048... [more]
XP_038900292.11.4e-20871.06uncharacterized protein At5g41620 isoform X1 [Benincasa hispida][more]
XP_022957750.14.1e-20870.65uncharacterized protein LOC111459204 isoform X1 [Cucurbita moschata] >XP_0229577... [more]
XP_023534122.12.1e-20770.61uncharacterized protein LOC111795778 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
Match NameE-valueIdentityDescription
Q66GQ22.9e-1826.86Uncharacterized protein At5g41620 OS=Arabidopsis thaliana OX=3702 GN=At5g41620 P... [more]
F4I8782.1e-0533.08Protein BRANCHLESS TRICHOME OS=Arabidopsis thaliana OX=3702 GN=BLT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K0X33.1e-20970.91uncharacterized protein LOC111490718 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1H0372.0e-20870.65uncharacterized protein LOC111459204 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K3155.9e-19270.50uncharacterized protein LOC111490718 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1H0016.5e-19169.98uncharacterized protein LOC111459204 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KEV92.7e-17362.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G425750 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G11590.11.2e-7539.36unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 22 plant structures;... [more]
AT1G50660.16.3e-3729.10unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT5G22310.14.1e-3634.32unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G20350.11.3e-3438.81unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: plasma mem... [more]
AT5G41620.12.0e-1926.86FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 235..255
NoneNo IPR availableCOILSCoilCoilcoord: 395..422
NoneNo IPR availableCOILSCoilCoilcoord: 299..334
NoneNo IPR availableCOILSCoilCoilcoord: 352..379
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 573..594
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..58
NoneNo IPR availablePANTHERPTHR31071:SF16OS04G0382800 PROTEINcoord: 11..565
IPR043424Protein BRANCHLESS TRICHOME-likePANTHERPTHR31071GB|AAF24581.1coord: 11..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0010212.1Lag0010212.1mRNA