Lag0010097 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0010097
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	SNF1-related protein kinase regulatory subunit beta-3
Location	chr9: 44620535 .. 44620962 (+)
RNA-Seq Expression	Lag0010097
Synteny	Lag0010097

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAACCCTTACTCTGAAGATAGTGTAAGCTGCTAAACCTAGGTGTTTCATCCACTAATTACATGAAATGGGGCTTTAAAGATGTATCCTTTCTACAGGATCAAACCACGGTCGTGGGTTTTGAAGTTCCAAAGTCACCAGATTCAACCTATAACAATATATACCTTGGGAGTGAAGACGAGGTACGTGATCCACCACTTGTCCCGCCTCATTTGCAGCTCACCTTACTCAGTCACCCAGCAAGCAGAGATGCTGGTGGAACTCTTCCATTGCCACAGAATGCGATTCTCAACCATCTTTACATCGAAAATCGGGAGACTCCACGTTCCGTTGTGGCTCTTGGATTCACACATCGTTTTCATTCAAAATATGTTACGGTTGTGCTTTACAAACCGGTTCATAGAAGAGGAAGTAGCAGGGCATAG

mRNA sequence

ATGAACAACCCTTACTCTGAAGATAGTGATCAAACCACGGTCGTGGGTTTTGAAGTTCCAAAGTCACCAGATTCAACCTATAACAATATATACCTTGGGAGTGAAGACGAGGTACGTGATCCACCACTTGTCCCGCCTCATTTGCAGCTCACCTTACTCAGTCACCCAGCAAGCAGAGATGCTGGTGGAACTCTTCCATTGCCACAGAATGCGATTCTCAACCATCTTTACATCGAAAATCGGGAGACTCCACGTTCCGTTGTGGCTCTTGGATTCACACATCGTTTTCATTCAAAATATGTTACGGTTGTGCTTTACAAACCGGTTCATAGAAGAGGAAGTAGCAGGGCATAG

Coding sequence (CDS)

ATGAACAACCCTTACTCTGAAGATAGTGATCAAACCACGGTCGTGGGTTTTGAAGTTCCAAAGTCACCAGATTCAACCTATAACAATATATACCTTGGGAGTGAAGACGAGGTACGTGATCCACCACTTGTCCCGCCTCATTTGCAGCTCACCTTACTCAGTCACCCAGCAAGCAGAGATGCTGGTGGAACTCTTCCATTGCCACAGAATGCGATTCTCAACCATCTTTACATCGAAAATCGGGAGACTCCACGTTCCGTTGTGGCTCTTGGATTCACACATCGTTTTCATTCAAAATATGTTACGGTTGTGCTTTACAAACCGGTTCATAGAAGAGGAAGTAGCAGGGCATAG

Protein sequence

MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRDAGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA

Homology

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BLAST of Lag0010097 vs. NCBI nr
Match: XP_022140750.1 (SNF1-related protein kinase regulatory subunit beta-3 [Momordica charantia] >XP_022140751.1 SNF1-related protein kinase regulatory subunit beta-3 [Momordica charantia])

HSP 1 Score: 224.9 bits (572), Expect = 3.5e-55
Identity = 107/117 (91.45%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MNNPYSEDSDQTTVVGFEVP SPDSTYNN+YLGSEDE RDPP  PPHLQ TLLSHPASRD
Sbjct: 1   MNNPYSEDSDQTTVVGFEVPNSPDSTYNNLYLGSEDEARDPPFAPPHLQHTLLSHPASRD 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA 118
           AGGTLPLP N ILNHLYIENRETPRSVVALGFTHRF SK+VTVVLYKPVHRRGSSRA
Sbjct: 61  AGGTLPLPHNVILNHLYIENRETPRSVVALGFTHRFRSKFVTVVLYKPVHRRGSSRA 117

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BLAST of Lag0010097 vs. NCBI nr
Match: XP_004145755.1 (SNF1-related protein kinase regulatory subunit beta-3 [Cucumis sativus] >XP_008465360.1 PREDICTED: SNF1-related protein kinase regulatory subunit beta-3 [Cucumis melo] >XP_008465361.1 PREDICTED: SNF1-related protein kinase regulatory subunit beta-3 [Cucumis melo] >XP_011657018.1 SNF1-related protein kinase regulatory subunit beta-3 [Cucumis sativus] >XP_016903370.1 PREDICTED: SNF1-related protein kinase regulatory subunit beta-3 [Cucumis melo] >XP_031742408.1 SNF1-related protein kinase regulatory subunit beta-3 [Cucumis sativus] >KAA0035235.1 SNF1-related protein kinase regulatory subunit beta-3 isoform X2 [Cucumis melo var. makuwa] >KGN46863.1 hypothetical protein Csa_020718 [Cucumis sativus] >TYK22390.1 SNF1-related protein kinase regulatory subunit beta-3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 224.6 bits (571), Expect = 4.6e-55
Identity = 109/117 (93.16%), Postives = 111/117 (94.87%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MNN YSEDSDQTTVVGFEVPKSPDSTYNNIY+GSEDE RDPPLVPPHLQ TLLS PASRD
Sbjct: 1   MNNLYSEDSDQTTVVGFEVPKSPDSTYNNIYVGSEDEARDPPLVPPHLQHTLLSQPASRD 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA 118
           AG TLPLPQN ILNHL+IENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA
Sbjct: 61  AGETLPLPQNVILNHLFIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA 117

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BLAST of Lag0010097 vs. NCBI nr
Match: XP_022958406.1 (SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita moschata] >XP_022958407.1 SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita moschata] >XP_022958408.1 SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita moschata] >KAG6606042.1 SNF1-related protein kinase regulatory subunit beta-3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035994.1 SNF1-related protein kinase regulatory subunit beta-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 224.2 bits (570), Expect = 6.0e-55
Identity = 105/115 (91.30%), Postives = 110/115 (95.65%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MNNP+ EDSDQTTVVGFEVPKSPDSTYNN+YLGSED+ RDPP VPPHLQ TLLSHPASRD
Sbjct: 1   MNNPFPEDSDQTTVVGFEVPKSPDSTYNNVYLGSEDDARDPPPVPPHLQHTLLSHPASRD 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS 116
           AGGTLPLPQN +LNHL+IENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS
Sbjct: 61  AGGTLPLPQNVVLNHLFIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS 115

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BLAST of Lag0010097 vs. NCBI nr
Match: XP_022996167.1 (SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita maxima] >XP_022996168.1 SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita maxima] >XP_022996169.1 SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita maxima])

HSP 1 Score: 222.6 bits (566), Expect = 1.7e-54
Identity = 104/115 (90.43%), Postives = 109/115 (94.78%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MNNP+ EDSDQTTVVGFEVPKSPDSTYNN+YLGSED+ RDPP VPPHLQ TLLSHPA RD
Sbjct: 1   MNNPFPEDSDQTTVVGFEVPKSPDSTYNNVYLGSEDDARDPPPVPPHLQHTLLSHPAGRD 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS 116
           AGGTLPLPQN +LNHL+IENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS
Sbjct: 61  AGGTLPLPQNVVLNHLFIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS 115

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BLAST of Lag0010097 vs. NCBI nr
Match: XP_038902942.1 (SNF1-related protein kinase regulatory subunit beta-3 [Benincasa hispida] >XP_038902943.1 SNF1-related protein kinase regulatory subunit beta-3 [Benincasa hispida])

HSP 1 Score: 218.8 bits (556), Expect = 2.5e-53
Identity = 106/117 (90.60%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MNN  SEDSDQ TVVGFEVPKSPDSTYNN+Y+GSEDE RDPPLVPPHLQ TLLS PASRD
Sbjct: 1   MNNLCSEDSDQNTVVGFEVPKSPDSTYNNVYVGSEDEARDPPLVPPHLQHTLLSQPASRD 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA 118
           AG TLPLPQN ILNHL+IENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA
Sbjct: 61  AGETLPLPQNVILNHLFIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSSRA 117

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BLAST of Lag0010097 vs. ExPASy Swiss-Prot
Match: Q9ZUU8 (SNF1-related protein kinase regulatory subunit beta-3 OS=Arabidopsis thaliana OX=3702 GN=KINB3 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-35
Identity = 72/115 (62.61%), Postives = 88/115 (76.52%), Query Frame = 0


Query: 1   MNNPYSEDSDQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRD 60
           MN+   +D + TTVVGFEVP SP S+YNN+Y  +EDE RDPP VPPHLQ +LL +  S +
Sbjct: 1   MNSQNPDDHEDTTVVGFEVPVSPVSSYNNVYSSTEDETRDPPAVPPHLQHSLLGNQGSME 60

Query: 61  AGGTLPLPQNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHRRGSS 116
                  PQN +LNHLYIENR+ PRSVVALGF+HRF +K+VTVV+YKPV RRGS+
Sbjct: 61  LAYA---PQNVVLNHLYIENRDAPRSVVALGFSHRFRTKFVTVVIYKPVQRRGSA 112

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BLAST of Lag0010097 vs. ExPASy Swiss-Prot
Match: Q9SCY5 (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX=3702 GN=KINB2 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.1e-26
Identity = 57/103 (55.34%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 10  DQTTVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASR-DAGGTLPLP 69
           D  ++ GFE P+SP+++Y+N+ LG+ED  ++PP+VPPHLQ+TLL+ PA+  D    LP P
Sbjct: 187 DIQSISGFEPPQSPENSYSNLLLGAEDYSKEPPVVPPHLQMTLLNLPAANPDIPSPLPRP 246

Query: 70  QNAILNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHR 112
           Q+ ILNHLY++  ++  SVVALG THRF +KYVTVVLYK + R
Sbjct: 247 QHVILNHLYMQKGKSGPSVVALGSTHRFLAKYVTVVLYKSLQR 289

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BLAST of Lag0010097 vs. ExPASy Swiss-Prot
Match: Q84VQ1 (SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX=3702 GN=KINB1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 6.5e-20
Identity = 48/99 (48.48%), Postives = 64/99 (64.65%), Query Frame = 0


Query: 13  TVVGFEVPKSPDSTYNNIYLGSEDEVRDPPLVPPHLQLTLLSHPASRDAGGTLPLPQNAI 72
           ++V FE P SPD +Y      +ED  ++P  VPP L LTLL    + +       PQ+ +
Sbjct: 188 SIVEFEAPPSPDHSYGQTLPAAEDYAKEPLAVPPQLHLTLL---GTTEETAIATKPQHVV 247

Query: 73  LNHLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPVHR 112
           LNH++IE   TP+S+VALG THRF SKY+TVVLYKP+ R
Sbjct: 248 LNHVFIEQGWTPQSIVALGLTHRFESKYITVVLYKPLTR 283

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BLAST of Lag0010097 vs. ExPASy Swiss-Prot
Match: Q9QZH4 (5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-09
Identity = 35/95 (36.84%), Postives = 50/95 (52.63%), Query Frame = 0


Query: 18  EVPKSPDSTYNN--IYLGSEDEVRDPPLVPPHLQLTLLSHPASRDAG-GTLPLPQNAILN 77
           ++  SP   Y        SE+  + PP++PPHL   +L+   +       LP P + +LN
Sbjct: 179 DLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLN 238

Query: 78  HLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPV 110
           HLY     T  SV+ L  THR+  KYVT +LYKP+
Sbjct: 239 HLYA--LSTKDSVMVLSATHRYKKKYVTTLLYKPI 271

[Close]

BLAST of Lag0010097 vs. ExPASy Swiss-Prot
Match: Q6PAM0 (5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab2 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 6.7e-09
Identity = 34/95 (35.79%), Postives = 51/95 (53.68%), Query Frame = 0


Query: 18  EVPKSPDSTYNN--IYLGSEDEVRDPPLVPPHLQLTLLSHPASRDAG-GTLPLPQNAILN 77
           ++  SP   Y        SE+  + PP++PPHL   +L+   +       LP P + +LN
Sbjct: 179 DLSSSPPGPYGQEMYVFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLN 238

Query: 78  HLYIENRETPRSVVALGFTHRFHSKYVTVVLYKPV 110
           HLY  + +   SV+ L  THR+  KYVT +LYKP+
Sbjct: 239 HLYALSIKD--SVMVLSATHRYKKKYVTTLLYKPI 271

The following BLAST results are available for this feature:

BLAST of Lag0010097 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140750.1	3.5e-55	91.45	SNF1-related protein kinase regulatory subunit beta-3 [Momordica charantia] >XP_...	[more]
XP_004145755.1	4.6e-55	93.16	SNF1-related protein kinase regulatory subunit beta-3 [Cucumis sativus] >XP_0084...	[more]
XP_022958406.1	6.0e-55	91.30	SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita moschata] ...	[more]
XP_022996167.1	1.7e-54	90.43	SNF1-related protein kinase regulatory subunit beta-3-like [Cucurbita maxima] >X...	[more]
XP_038902942.1	2.5e-53	90.60	SNF1-related protein kinase regulatory subunit beta-3 [Benincasa hispida] >XP_03...	[more]

BLAST of Lag0010097 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZUU8	1.4e-35	62.61	SNF1-related protein kinase regulatory subunit beta-3 OS=Arabidopsis thaliana OX...	[more]
Q9SCY5	6.1e-26	55.34	SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana OX...	[more]
Q84VQ1	6.5e-20	48.48	SNF1-related protein kinase regulatory subunit beta-1 OS=Arabidopsis thaliana OX...	[more]
Q9QZH4	1.8e-09	36.84	5'-AMP-activated protein kinase subunit beta-2 OS=Rattus norvegicus OX=10116 GN=...	[more]
Q6PAM0	6.7e-09	35.79	5'-AMP-activated protein kinase subunit beta-2 OS=Mus musculus OX=10090 GN=Prkab...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006828	Association with the SNF1 complex (ASC) domain	SMART	SM01010	AMPKBI_2	coord: 20..109 e-value: 2.2E-27 score: 107.0
IPR006828	Association with the SNF1 complex (ASC) domain	PFAM	PF04739	AMPKBI	coord: 39..108 e-value: 1.7E-20 score: 73.1
None	No IPR available	GENE3D	6.20.250.60		coord: 13..109 e-value: 1.8E-23 score: 84.5
IPR043554	SNF1-related protein kinase regulatory subunit beta	PANTHER	PTHR46316	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-1	coord: 1..115
IPR030067	SNF1-related protein kinase regulatory subunit beta-3	PANTHER	PTHR46316:SF5	SNF1-RELATED PROTEIN KINASE REGULATORY SUBUNIT BETA-3	coord: 1..115
IPR037256	ASC domain superfamily	SUPERFAMILY	160219	AMPKBI-like	coord: 12..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0010097.1	Lag0010097.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043562	cellular response to nitrogen levels
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009744	response to sucrose
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0005515	protein binding